LOCUS       NZ_CP073886            13570 bp    DNA     circular CON 16-APR-2024
DEFINITION  Staphylococcus epidermidis strain V1933625 plasmid p1V1933625,
            complete sequence.
ACCESSION   NZ_CP073886
VERSION     NZ_CP073886.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699895
            Assembly: GCF_019330125.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 314x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019330125.1-RS_2024_04_16
            Annotation Date                   :: 04/16/2024 08:04:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,688
            CDSs (total)                      :: 2,607
            Genes (coding)                    :: 2,528
            CDSs (with protein)               :: 2,528
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 42 of 79
            Pseudo Genes (incomplete)         :: 43 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 24 of 79
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073886.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="V1933625"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p1V1933625"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(12252..13570,1..226))
                     /gene="qac"
                     /locus_tag="KFV30_RS00005"
                     /old_locus_tag="KFV30_00005"
     CDS             complement(join(12252..13570,1..226))
                     /gene="qac"
                     /locus_tag="KFV30_RS00005"
                     /old_locus_tag="KFV30_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            405..971
                     /gene="qacR"
                     /locus_tag="KFV30_RS00010"
                     /old_locus_tag="KFV30_00010"
     ARG             406..968
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1222..1782
                     /locus_tag="KFV30_RS00015"
                     /old_locus_tag="KFV30_00015"
     CDS             1222..1782
                     /locus_tag="KFV30_RS00015"
                     /old_locus_tag="KFV30_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            2201..3112
                     /locus_tag="KFV30_RS00020"
                     /old_locus_tag="KFV30_00020"
     CDS             2201..3112
                     /locus_tag="KFV30_RS00020"
                     /old_locus_tag="KFV30_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(3289..3387)
                     /locus_tag="KFV30_RS00025"
                     /old_locus_tag="KFV30_00025"
     CDS             complement(3289..3387)
                     /locus_tag="KFV30_RS00025"
                     /old_locus_tag="KFV30_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            3551..4249
                     /locus_tag="KFV30_RS00030"
                     /old_locus_tag="KFV30_00030"
     CDS             3551..4249
                     /locus_tag="KFV30_RS00030"
                     /old_locus_tag="KFV30_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            4400..5104
                     /locus_tag="KFV30_RS00035"
                     /old_locus_tag="KFV30_00035"
     CDS             4400..5104
                     /locus_tag="KFV30_RS00035"
                     /old_locus_tag="KFV30_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(5153..5386)
                     /locus_tag="KFV30_RS00040"
                     /old_locus_tag="KFV30_00040"
     CDS             complement(5153..5386)
                     /locus_tag="KFV30_RS00040"
                     /old_locus_tag="KFV30_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(5463..5876)
                     /locus_tag="KFV30_RS13500"
                     /old_locus_tag="KFV30_00045"
     CDS             complement(5463..5876)
                     /locus_tag="KFV30_RS13500"
                     /old_locus_tag="KFV30_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            complement(6150..8072)
                     /locus_tag="KFV30_RS00050"
                     /old_locus_tag="KFV30_00050"
     CDS             complement(6150..8072)
                     /locus_tag="KFV30_RS00050"
                     /old_locus_tag="KFV30_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            8281..8550
                     /locus_tag="KFV30_RS00055"
                     /old_locus_tag="KFV30_00055"
     CDS             8281..8550
                     /locus_tag="KFV30_RS00055"
                     /old_locus_tag="KFV30_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            8567..8806
                     /locus_tag="KFV30_RS00060"
                     /old_locus_tag="KFV30_00060"
     CDS             8567..8806
                     /locus_tag="KFV30_RS00060"
                     /old_locus_tag="KFV30_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(9031..9966)
                     /locus_tag="KFV30_RS00065"
                     /old_locus_tag="KFV30_00065"
     CDS             complement(9031..9966)
                     /locus_tag="KFV30_RS00065"
                     /old_locus_tag="KFV30_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            10407..11168
                     /locus_tag="KFV30_RS00070"
                     /old_locus_tag="KFV30_00070"
     CDS             10407..11168
                     /locus_tag="KFV30_RS00070"
                     /old_locus_tag="KFV30_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            complement(11487..12047)
                     /locus_tag="KFV30_RS00080"
                     /old_locus_tag="KFV30_00080"
     CDS             complement(11487..12047)
                     /locus_tag="KFV30_RS00080"
                     /old_locus_tag="KFV30_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     ARG             complement(12253..13568)
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic"
                     /identity="99.77"
                     /coverage="85.21"
     ARG             complement(3..226)
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic"
                     /identity="98.67"
                     /coverage="14.59"
     Typing          complement(9032..9966)
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6220..8072)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 cactcaatgg aattataaag ccagctaaaa caagagagta tatatcaact atccataact
       61 gttgggtacc agaaggctct aactctctta ctaattccgg taaagccata attaatattg
      121 tcatatccat tgtaacaaca aacaaactaa cagctaatac tactagtgca gtccatcttt
      181 tttttgatgt catcatatca gtagtttttg taaaaaatga aatcatttaa gttccctcca
      241 atccttatag accgtgcgat cggtctataa ggattataat ctattttttt tacatattac
      301 aacaaaaaat cttgcgaaat ttaaatggct gtattgtgtt atacaattat tctttgaatt
      361 ttttgtgcta tcattgatag tacttaatac tcattaaagg cgtgatgaac ttgaaagata
      421 aaatactagg tgtcgcaaag gaattattta taaaaaatgg atataatgcc actactactg
      481 gagaaattgt taaattatca gaaagtagta aagggaatct ttattatcac tttaaaacaa
      541 aagaaaatct atttttagaa attttaaata tagaagaatc taaatggcaa gaacagtgga
      601 aaaaggaaca aatcaaatgt aaaactaata gagaaaaatt ttatttatat aatgaactat
      661 ctttaacaac cgaatactat tatccacttc aaaacgcaat aattgaattt tacactgaat
      721 attataaaac taatagcatt aatgaaaaaa tgaataaatt agaaaacaaa tatatagatg
      781 cttatcatgt aatttttaag gagggtaatt taaatggcga atggtgtatt aatgatgtta
      841 atgctgttag taaaatagca gcaaacgctg ttaatggaat tgttaccttt acacatgaac
      901 aaaatattaa tgaaagaatt aaacttatga acaagttctc tcaaattttt ttaaatggac
      961 ttagtaaata attatatata tattcttgaa ataccaataa atattatttc taaattacgt
     1021 tcgtttttga cacaaaacct cttaacttaa tattaagagg tttttcttgg atatttaagt
     1081 ctataaactt gtgttataat caatgtatca taaatatcga aaatcatgag tttttagatg
     1141 ctgacagacg tgtttattta catcgagaac tatatttcat tagtaagtac ccataaaacg
     1201 attaaaaaag gtggtgttca aatggctaaa attggttatg cacgtgtgtc aacacaagat
     1261 caaagtcttg atggacagat tgatacactt gaggaatatg gttgtgaacg tatctttagc
     1321 gagaaagcga gtggtcgtaa aactaaaaga acggaacttg ataagtgttt agattattta
     1381 cgtgaaggag atattctagt tgtctataaa ttagatcgtc ttggacgtac aacaaaacaa
     1441 ttaattgagc tatctcaatg gttggatgaa aatagcattg atttacatat tatagatatg
     1501 aatgtgtcaa cgaaagatgc gatgggcaag atgttcttta ccatgatgag tgcattcgct
     1561 gaacttgaag ctaatttatt aagtgaacga actaaaaaag gtttggaagc tgcaagagca
     1621 agagggagaa aaggtggaag accttcacta ccagatcata agaaaagaga gatcaaattc
     1681 ttatatgatg aacaaaagct gtctggtgaa gaaattgctg aacaaacagg agtgagtcgt
     1741 tctactgtat ataggattat taaagagtca agaaaaatat aaagtactaa attaaagttt
     1801 taatataccc tttaattgaa aaatcttctt aaaaagtgtc tttaacttca gtccccttta
     1861 acaatccact cgcttaatag ctttaaattt ctaagcatta cttgagcttt gaaaattttc
     1921 atcgattcat taaccgacat aactggattt tgactgttaa tattcacctt gtcctctcca
     1981 attaacaaaa aagcaaaagc atataaacaa catttgttgt ttatatgctt ttgcttgcat
     2041 tttcttctat agtatgctat aatgaaaagg ttaagtctat ctatacatca aaagcaataa
     2101 acttccacct agaataacaa cttattgtaa atcgacaatt acaataatac atcattctat
     2161 attggaagta aacaaaaaac ttacagaagg atgatataga gtgacactag tatgtaccaa
     2221 attcaatcat gatgctaaat caactgatta tttaaatatt tctattgaag aagacactct
     2281 tcaatatata gacccaaacc ttattaaagt aatatctaat agagaagttg gaaaagttgc
     2341 gactttggct caaagagcac aagagcaaat tgatgttttt tttgaaaaaa ttatcgatat
     2401 acatcttagt gatttttctg atacccaaaa gcgtaatgaa tttagaaaat tattctctca
     2461 tttttcagaa ccacaccatt tacgcttagg tttttctcag aaaggtaata caggaaaagg
     2521 tactacagca tctgaattga ttaatctatt tatgaatgaa gatattcttt ccattatctt
     2581 aaatgatgac ggattaacaa tacctcaaaa agcaccatta atcagaaatt ttggtgatga
     2641 taaattatca gatttaacaa gtaatataat catgaatgtt ataattgatt ttaataaatc
     2701 catgattcag tcatttccag aaatgaaatc gtttctttct aaagaaactg tgacatatta
     2761 ttatttctct actaacggta tatgggaaac ttatgatttc actccatttc tttttgatgg
     2821 taataaaact ttattagttc caaaattatt cactacttat aatcagacat ctagtttaga
     2881 tttgatgata agagtataca ttgaagaaga aatcattaga ttgcaaaaac aacgtaatac
     2941 aaccaaaaaa atgacaaaaa aacaatttat caatgaattt ataaaaggac atcgtatgga
     3001 taatatgacc tatttctttt taaacacttc ttcaaaatca cacaaaaaat ttcaaaaaga
     3061 attagattca aaagccaatg aaaggggtaa caataacaaa gatgataatt agtacttact
     3121 aatcagtata gtatagactt aacatactaa ttattcaatt ttgcattcct ccacactatg
     3181 atgttataat ttagataaca aatatgtgtt gaacatggta aacaccgagc ccgataacta
     3241 ttccagtagt tatcgggctc tttttggtgt tattactgtg tcgtcgcttt attgtttatc
     3301 acgattattc aaccaatgtg aaaacaacgt gattataacc cctactataa taggagctac
     3361 tatagataca aatatgatgc tgaacatggt gtcacctcct tccatgctaa accagcacag
     3421 aagtagaacg acgactttta tattatatca tttgtgaatt gttaagcata tgcacttccg
     3481 ataagtcatt ttatgtttaa tgtatctatg tgataatgaa aatacaaatt ctattaaagg
     3541 agataatttt atgaaaaaac gatcattact tccaacttta ttattggcta gctcattaac
     3601 attcacaggt ttttcagcga cagccaatgc tgaggatggt gccaaaaccg tatacggaga
     3661 gaacttacca aatagcgcag atacaaatgg agatggttgg gctaatgtag acttcaatac
     3721 ttcatccctc ccacaatctg cacaaaatca aataagtgaa ctttctaaac agaaagattc
     3781 aggggaatta actcaaatcg agtataatga gaaagtttca agtgtattta atgaatatca
     3841 atcacaaagt actgatatac aaaataatga gagaactcaa caaggtaaaa gacctttcgg
     3901 aggggtgata cctaattgta tgactcaaga agaataccaa gaattagaaa gtcatgttcc
     3961 taaccctaat agtgtatcca cagaaaaata taatcaaatt gtaaaaaatg aaagtcaaaa
     4021 gatagctaat gaagaaaata aaactattaa tacacctcaa ggtgtgttga cacctgtaac
     4081 tgaaaataaa aacaagagtt ctgaaggaat gaaaaatcaa gattcaaact ctcaagtatt
     4141 accagaaaca ggagaaagca accaaagtga tatattgtca ataattggcg cttctggact
     4201 actaatatta ggtataagtc ttttaatatt cagaaaaaag gcaatataaa aattgataaa
     4261 ataaccaccg tgtttatgta aaacatgtgg ttattttatt atatatttaa tacatatttt
     4321 tcaactcatg taatttacgt tttcttgctg aaaaaatatt aacataatct aatttgaaat
     4381 actgaaaagg agttgtaata tgaaaaaaat cattttacct atattaattg ttagtttatc
     4441 ggtaatttta gtgagttgta ataataacga ttcagaacat cacaatgata ataattctag
     4501 tgatactaac acctcagaaa acaaagataa tgaagcatca aatgaaattg acgaaaaaac
     4561 taacacatct gatagtgcgg ataattcaga aagtagtaat aaacaaaata aagaaaaaaa
     4621 tgataataaa aaacaagaaa aaaataatga tagtaataag aagcaacaat ctactgataa
     4681 aaataaaagt tacttaaata attttaactc gaaagaaatt gaatatgcac gagtttggta
     4741 tcagttaata tctacaagaa atgatttaaa aggtattaaa aatgtttatg ttacagaaat
     4801 accaaaaggc tcaaaagtaa atccacaagc caaaaatagt gcaatttata aagaaaaagt
     4861 tgtgaaatta gaagctccta tgagagctgg tgggtctgta acatatagca gtaatggtga
     4921 tggaacaatt aatgtatata ataatatccc ttataaatgg gaaaattcac aaaatagtga
     4981 ttatagtcaa atgaataaga taacaaaaaa agcaatagag gagaatattg aaacagtcta
     5041 tattaaaccc tacgacaata aaacagtagc taagttagca agtaaaatta gatataataa
     5101 gtagttagta gattattaaa aaggcgccta aaattaggcg tcttttttat cattagttca
     5161 aaagataagc ataacgttcc atgtaacagc atataaagta atgaatcaat tcatcactat
     5221 ctaatgccac cattgttagt tcagccttgc atggtttaac gtatctcaca taatggtaca
     5281 aatcttcaaa tagtgcctgt ttttcgggca ttttaaacac cttgtgtaca ttaaaaccta
     5341 tttcttctaa tcgctttgta atcacgccga aaacgaatgt gttcatgtga aatcactcca
     5401 tcatttttaa tattcacatg tacactaaaa aaaatccgat acatcggctt tttatttttg
     5461 gtttaattta tttctatatt gttttcacta tcgctcggac gcataagtgt taaaacacct
     5521 tctaccatac ctatcacacc cggtattccc gtccaacaaa atagtaaata aagaagagca
     5581 tttttatttt tcttaacata aaacttatga gccccgaaac ctcctagaaa aaatgctaat
     5641 gcagagtaaa catatttatt aacttgcatg atttccactc ctttaattaa ttgatttaga
     5701 gaaacattaa ataatttagt gatttctaaa attgttttta agtcgggatt acttacttct
     5761 ctttcccaat tagaaataga atgtctcgat acccctaact tttcagctaa tttttcttga
     5821 gacatgtttt tactttttct taacttttgt atttgatgtc caatttttgt ttccaaatta
     5881 tttttctcct taactttaag atatatttaa actcaatttt ttctatcgat atttatgaac
     5941 attagtattt tttatgctga aatatgtgaa caatcgcata atatcaaggt ttttctataa
     6001 ttatacacgt tttaaaatac attgtgataa tacatagaat gtaccttatc taagtatata
     6061 aatgttttct ttaaaccaaa gctaatttac cacattgggt taagtttctt attattaaag
     6121 aatgaacttt gtatgatttc aactgcttac tatcttctca tttggttatt tctctcttgt
     6181 ttcttcttct ttttctgatg attaatattg ttttgctttt cagtttcgtt cgcatagtac
     6241 ataagaaagt cactagcatt tatcgttggt aaattaatgt gattagtttg ttcattttca
     6301 tgttcaatac gattgtcatg atcatttcta tctacttgat taatatgttt ttcgataact
     6361 tcttcttgat cgacatgatt atatttctca agtttaatga gtaattgaac tttttcatta
     6421 ggtgtttggt ctttgacttt ttcaaagttt ttatcgccta tatctattct taaatcatca
     6481 tcaatatgtt ctttataact atcaatttct ttttcttttt ccttaataaa ttgcaactca
     6541 acgttatatt caaagctatc tttttctaaa tttttaagat gtttttgact aaagaaaata
     6601 tctttactcg cttttttata agtatcttct agcaatacat cattaggaat attgttattt
     6661 tgtttgaaca ggtattgaac atgagttgct tttataatat tacctttttg tgcctcatca
     6721 acaataaatg ctttttcatc atggctaaaa tgatgatgag atttagggta catttgctga
     6781 acaatgagat gatttttttc actttcagtt agataaacat ctttagctga ttgataaatc
     6841 tctttacgtt tttcctctct actttctaat ttattcttct cattgagtaa cgctttatag
     6901 ttagtttcaa cagtattaac aaactcttgt tgactatatc ctttactttc aatttctttt
     6961 ctaaactgat aattttcatc ataattttta gcatagaatt ttaattgacg tctcttaatt
     7021 tcatttttaa gtaaatcatt ttgaatttta cgaccaaagg tagacgcact gtcaaacgta
     7081 tttttaattg tttctttcgc ctctgaatag ccaacatcac gttgatatct atcaaataaa
     7141 atagaataac tttgttcatt tttacttact tgttcgttag cattttgaat aaataatcta
     7201 atttcatttt gtgcttgttc tttctcttta tcactaaata attgtgatac ttcgccacga
     7261 ttatatcttc taatttgttc atttttctgt ccgtaataag taacgggttg gtactcgata
     7321 ccctcttttt cacagcgttt tctttcattt tgttctaatc tatattcttc tcttgtaagt
     7381 ggaatttcgg caattttttc taaaccttgc gcttcaaaag aacgactatc atatcgtctt
     7441 aaactttgtt cacgttctga gtattgattt aattttaatt cccaatgctt acgaacttct
     7501 ttgaaatcaa agtcggcaaa tcgttcttgt tttctaactt tattaccatt tttatttaaa
     7561 atcgcattgc cattttcatc atattgcgtt ctcgtttttg tctttttccc ccaagtaccg
     7621 tctgcattaa acggtctttg agtaagtaga acatgggcgt gtgggttgtt tatatcatct
     7681 ctatgaattg aaatatcagc aaccattcct ttgtctacaa actcactttt tataaaatct
     7741 ttagttaatt cagtttgtac ctctctatca aaatcattag gcaaacttaa taagacttct
     7801 tttgctaatc taggattttt tgttttcgca ttattctttt caactttatc aacttcattc
     7861 cataaacgct gtctatcttt agtccattcg ggtgcatgtt gaggagttaa aatcatgctt
     7921 tcaggcttaa tagtatgatt tctaaactta attttttcat ctgtacgttc gctatataat
     7981 gcttcatcag aacgataaga cgctgacgca acgatatttt ttgttgctcg attaatgtta
     8041 gtcactctca acataaagta acctttattc atgattttac ctcctctctt ttctgttttt
     8101 ttacccctgt gggaactata atgatcaacc agtatggttg cccattttta tgccaatttt
     8161 caatttgcat gtaaatgggc agtgcagtgt cttaagaact ggttatatta taccatggat
     8221 gtgcgactta cccaatgaat tatggtatgc tttatataga taaactaagg gagtttttta
     8281 atgtctaaaa ttaaacaaac agatattaca aaattaagtc aattagaaaa acaaattgca
     8341 caactgaaag agcaaaagaa aaaacaagaa gaacaactat ctaataaaat tggtggttat
     8401 tttttaaata aacttgattt aaatatgatc aactctagtg atgaattgta taaaattatg
     8461 gataaagtca ttggagaatt taataaagat atcgaacaaa atagcagcaa cgagaacaag
     8521 gaaaatttaa cagaagacaa aattaactag aatagtaagt gatacgatga atgattttaa
     8581 acatctcaaa aaaataaatg ctgaaattga aaaagcagaa ttaagaaagc atagattaaa
     8641 aaatttagat agaaaagaaa gagcacatag actgattcga aaaggtgcaa tgcttgaaaa
     8701 gtattttgaa tgtgagcatt taagtcctga tgaaacagaa gaattgttaa agatgtattc
     8761 caactacatt aatacaaata aacccaataa atataaaaag aagtaattta tcatgcttac
     8821 aaatacttgg gtttcactaa aaagaaaaaa ttgctgatag tagtaacaat gaataataat
     8881 tgtttataaa aatgggacta aaaaagaaaa gttttagtcc cacttactta attaattaca
     8941 tttcattgaa aataaagttc gcttacagtt aagagaacat ctaattaaag atattctcta
     9001 attggatcat tgttggctta ttaagttgta ttattgatta aataaagaag tgattggtaa
     9061 atgatcacgt cgtttattaa gcgtatgtac ttctgcaaat tcagatttaa gagatttcat
     9121 taagtaccct tgcattgctt caacggtttc atttttttga attaatgtag ctttaaaacg
     9181 cttaagaaca ttaacaattt caaattccat atcttctaat aaataatatg catcaataga
     9241 attgttgaag gatgttttgg cttttaataa cacagattta ataagtttaa tttcagatac
     9301 agaaaagtta cttaaataac tttgaatact ttgtggtagt tcttcaagta attgaaattt
     9361 taaagcttca tcattgaatt ttgaatagta gtgatttgaa tgatttgtat gattactaga
     9421 atatgttaat ttcctatcat tcaaatcata catatcatta taattagtct taatataatc
     9481 agtgtcatta taatcagtat cattagactg tgaatttgga agttcttgac cacttaaatt
     9541 cacagttcta gaacttccaa attcattgtt ctggaactgt gaatttggaa gttccttgat
     9601 atgactggct tcaatgtcat aattaggttt taataggtat aatctatttg gtttattaag
     9661 gccaacacgt tcttgagtta gtaaattaaa ttttattaac tcttttttta attttataat
     9721 tttttgttct gcataaccta agtattcaca caaatgttta ttactgaaaa taaagtaaat
     9781 attattattt ttatctaccc attcattttg gagggataat tcaaaacgat cttttaataa
     9841 cgcatacata attttggctt ctgcagacat tttcttaaaa tatgcatctt caaataaata
     9901 tttaggcagt tgataaaatc tttcagtttt tacttgtgat aaattatatt tttcaaaatt
     9961 gggcataata aaaacctcat ttccattttg ggattgaggt aataaaacaa tttttacgta
    10021 agttatatgt tttgtgcttg cacttagatg attagatata tataatacaa gtatcaaaaa
    10081 aatatataaa caaacgagtg cgaatcgttt tattaccgag agtcgatgct acgccaatag
    10141 catcgactct tttaatattt ataaagtaat attaacactt ttaatggcgt atataaagag
    10201 ttttaaatgt aattaaaagt acacctaaaa ctaccaaacg tataattcgc tattttagca
    10261 cctaattaag tggcgaaaat attttaagga tagataatta ggcatttagg tagttaatag
    10321 atagtaaatt tagtactaaa tgatgaaata aattttgtta ggtggtaaaa tttaggtact
    10381 aaattaggta cctaaggggg gatgttatga aaactattaa aatgattgct gatgaattag
    10441 gtgtaactaa acaaactatt gttaataatg ctaaaagatt aaatatatct tttgaaaaag
    10501 aaaatggaat taattatatt aaagataatg attatttaaa aattatagaa aaaatcacta
    10561 agaaagagaa aacaactcat aataaagaat cagtaaaaaa tgaattattt aatgaaaata
    10621 ctgcaaaagt tgaaaagtat atacataata attcaaatag ttctgagaca ttaaaaacaa
    10681 aagtaaacga attagaaaag caaatagaaa tctttgaaac tagagctaaa aatgatgaaa
    10741 aatatattga gaatctaact aaacaattag atcaacaaaa tagcaatgtt aatacattaa
    10801 ataaactgtt ggagaatcaa caaatattat ctttagaaaa caataaaaaa attcaaaaac
    10861 tagaaagcca attagaagaa gaaagacagc ttaaatattc ttttgataca acgatgaata
    10921 atagacagaa tattaatcca caagaagcaa cttttactga agaatctcaa aatatcaacc
    10981 aacaacaaaa agaatgtcaa ccaaccgaag taaatcataa aaatataact gaagagaata
    11041 agcatgacag tacagatgaa ttaacaaaag aggataagtt tgttgaaaaa acgaagaacg
    11101 attccagtga taatagggag gtagaaccac ctaaaaagag attttggagt cgtttatttg
    11161 gtaactaatt gcaattaaaa aggcacacaa gttcatgaac ttgttgtgcc tttttaatgt
    11221 gaatcaaact tctgataatc atcagttatg ttaaactcta acaaaaacta aattatcaga
    11281 agaattatct agggtattaa acataggtaa atcaattttc tatcgccaat agtataaaca
    11341 tcatccatat taatctgaag atagctacat aattctttaa cagcatagcc tttatttgta
    11401 taaggcgcca taaactctat taaatatggt ttgttttgag gtttctatgt ttaatcattt
    11461 aatttttcat tatataaatc gaataattat aaaaaatcat catacttttt attttttaaa
    11521 aatatttgaa aatttaatag taatccaatt ataagatcaa atatcattat cattataatc
    11581 aaggcaacta taatcactgt gacactcgtt acataacaag ctagtaatct ccagataaaa
    11641 ttgacatttt caccaattcc ataacttaac atattacaaa gtagtaaaag aataataata
    11701 cctatactgt aaattaaaat tgtcagtgta tattttttaa taactctatt aggaaacaat
    11761 agttcatcta ttaggttctt tttcaccagt atctctcgtt caattctatc tttgtcacag
    11821 attttgattt tactattttt tattttttcc tctaataaaa taacctcgaa cgatctgcta
    11881 atttttaata aatatgcagg aaaagctaat gttaagcata atgtaattaa ttcatttaca
    11941 ttgttcatgt aacttataaa atagatgaaa gttgctacta gtgctcctaa aaatatagtt
    12001 atattaagtt ctacatcaaa tattttaaaa atatctgctc tattcatcag ttaatcatcc
    12061 tttcaaggtt tattaatact aataaattat tagatatagg tatatcatat tattaattta
    12121 agaaattgtc ttttataatt ttacttaata ataaaaaagt agaaccattt aaaattaatg
    12181 gttctacttt ttaactatta ctaattttaa aaataaacta atctacaata tctaaaaata
    12241 tatgtttagt actatttttg tttagttata tcaagtgatt tgggaattaa caaatagaca
    12301 actattgaaa tgataatcat gataatccca cctactaaag cagttgctac aaaagcatca
    12361 ttaaatgatg ttacagcctc gtttgcaagc tgttttatcc ccgtagcttt agctacttcg
    12421 acagcgccca ctacagattc ttcagctaca tgagctaaat ctccaactat accttttgat
    12481 gaaaaagatg aaatatctaa aaatacacga taaagcatag aagataggct accaagtact
    12541 gctacaccaa aaacatttcc aaggtcatac atagactctt caacagcagc tgcattacct
    12601 gcttttgatg taggtgtttc taacattatt agagcagatg caactgctag tgaagccata
    12661 ccagctccaa ctaaaattaa tgctaaagcc attgtagaat atgataatgg atgaccaaag
    12721 aaatacataa taaacatgcc aatggctgca attccaattc cggaaggtaa cactattttc
    12781 ggtccaaatc gcgccgctaa tccgggtgca attggtgcaa acaccatatc tcctattgcc
    12841 ataggtaata ggtataagcc agctttaaaa ggagaaagtt cttccacaac ctgtaaccat
    12901 tgtgaagcta ataacaaaac agatgccatt gcaaaaattg tcataaatgc agcaattgta
    12961 ccagctgaaa atgatctctt tttaaaaagt cttacgtcta acattggatc agaacttgat
    13021 aaattacgtt taacaaatat cactatcatg gtaattgcta atactattac aacccatgga
    13081 ataatatctg ctagtccttc ttttgaaaat tctttgatac tccataccag tccaatcatg
    13141 cctgcaattg ataaaattgt agaaggaata tcccacgagt gagacttttc ttttgataac
    13201 ttagactctg gtaataaaaa taaacctgct acaactgcta ttatcgcaaa cggtacatta
    13261 attaaaaatg ccgagtgcca tgaaaattgc tcaagtaaag ctcctccgat aattggtcca
    13321 aaaacagcac ctatcgatga agcgattgac catacagcta atgcagtggc cctttcttta
    13381 gggttttcaa aaattactct tatcattgaa agagtagttg gcattattaa agcacctgca
    13441 ataccaagta aaaatcgaat agctattacg aactctgcac tttctgcgaa aaatatagct
    13501 aatgaaacga ggccaaataa agcaaatcca gttaataatg ctttttttct tccccattta
    13561 tcagcaaagg
//
