LOCUS       NZ_CP073884            13570 bp    DNA     circular CON 17-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1287175 plasmid p2B1287175,
            complete sequence.
ACCESSION   NZ_CP073884
VERSION     NZ_CP073884.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699894
            Assembly: GCF_019330105.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 117x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019330105.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 06:52:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,708
            CDSs (total)                      :: 2,627
            Genes (coding)                    :: 2,545
            CDSs (with protein)               :: 2,545
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 42 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 28 of 82
            Pseudo Genes (multiple problems)  :: 24 of 82
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073884.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1287175"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p2B1287175"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(12284..13570,1..258))
                     /gene="qac"
                     /locus_tag="KFV54_RS13215"
                     /old_locus_tag="KFV54_13225"
     CDS             complement(join(12284..13570,1..258))
                     /gene="qac"
                     /locus_tag="KFV54_RS13215"
                     /old_locus_tag="KFV54_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            437..1003
                     /gene="qacR"
                     /locus_tag="KFV54_RS13220"
                     /old_locus_tag="KFV54_13230"
     ARG             438..1000
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1254..1814
                     /locus_tag="KFV54_RS13225"
                     /old_locus_tag="KFV54_13235"
     CDS             1254..1814
                     /locus_tag="KFV54_RS13225"
                     /old_locus_tag="KFV54_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            2233..3144
                     /locus_tag="KFV54_RS13230"
                     /old_locus_tag="KFV54_13240"
     CDS             2233..3144
                     /locus_tag="KFV54_RS13230"
                     /old_locus_tag="KFV54_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(3321..3419)
                     /locus_tag="KFV54_RS13235"
                     /old_locus_tag="KFV54_13245"
     CDS             complement(3321..3419)
                     /locus_tag="KFV54_RS13235"
                     /old_locus_tag="KFV54_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            3583..4281
                     /locus_tag="KFV54_RS13240"
                     /old_locus_tag="KFV54_13250"
     CDS             3583..4281
                     /locus_tag="KFV54_RS13240"
                     /old_locus_tag="KFV54_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            4432..5136
                     /locus_tag="KFV54_RS13245"
                     /old_locus_tag="KFV54_13255"
     CDS             4432..5136
                     /locus_tag="KFV54_RS13245"
                     /old_locus_tag="KFV54_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(5185..5418)
                     /locus_tag="KFV54_RS13250"
                     /old_locus_tag="KFV54_13260"
     CDS             complement(5185..5418)
                     /locus_tag="KFV54_RS13250"
                     /old_locus_tag="KFV54_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(5495..5908)
                     /locus_tag="KFV54_RS13655"
                     /old_locus_tag="KFV54_13265"
     CDS             complement(5495..5908)
                     /locus_tag="KFV54_RS13655"
                     /old_locus_tag="KFV54_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            complement(6182..8104)
                     /locus_tag="KFV54_RS13260"
                     /old_locus_tag="KFV54_13270"
     CDS             complement(6182..8104)
                     /locus_tag="KFV54_RS13260"
                     /old_locus_tag="KFV54_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            8313..8582
                     /locus_tag="KFV54_RS13265"
                     /old_locus_tag="KFV54_13275"
     CDS             8313..8582
                     /locus_tag="KFV54_RS13265"
                     /old_locus_tag="KFV54_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            8599..8838
                     /locus_tag="KFV54_RS13270"
                     /old_locus_tag="KFV54_13280"
     CDS             8599..8838
                     /locus_tag="KFV54_RS13270"
                     /old_locus_tag="KFV54_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(9063..9998)
                     /locus_tag="KFV54_RS13275"
                     /old_locus_tag="KFV54_13285"
     CDS             complement(9063..9998)
                     /locus_tag="KFV54_RS13275"
                     /old_locus_tag="KFV54_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            10439..11200
                     /locus_tag="KFV54_RS13280"
                     /old_locus_tag="KFV54_13290"
     CDS             10439..11200
                     /locus_tag="KFV54_RS13280"
                     /old_locus_tag="KFV54_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            complement(11519..12079)
                     /locus_tag="KFV54_RS13290"
                     /old_locus_tag="KFV54_13300"
     CDS             complement(11519..12079)
                     /locus_tag="KFV54_RS13290"
                     /old_locus_tag="KFV54_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     ARG             complement(12285..13570)
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic"
                     /identity="99.77"
                     /coverage="83.27"
     ARG             complement(2..258)
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic"
                     /identity="98.84"
                     /coverage="16.73"
     Typing          complement(9064..9998)
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6252..8104)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 tgcttttttt cttccccatt tatcagcaaa ggcactcaat ggaattataa agccagctaa
       61 aacaagagag tatatatcaa ctatccataa ctgttgggta ccagaaggct ctaactctct
      121 tactaattcc ggtaaagcca taattaatat tgtcatatcc attgtaacaa caaacaaact
      181 aacagctaat actactagtg cagtccatct tttttttgat gtcatcatat cagtagtttt
      241 tgtaaaaaat gaaatcattt aagttccctc caatccttat agaccgtgcg atcggtctat
      301 aaggattata atctattttt tttacatatt acaacaaaaa atcttgcgaa atttaaatgg
      361 ctgtattgtg ttatacaatt attctttgaa ttttttgtgc tatcattgat agtacttaat
      421 actcattaaa ggcgtgatga acttgaaaga taaaatacta ggtgtcgcaa aggaattatt
      481 tataaaaaat ggatataatg ccactactac tggagaaatt gttaaattat cagaaagtag
      541 taaagggaat ctttattatc actttaaaac aaaagaaaat ctatttttag aaattttaaa
      601 tatagaagaa tctaaatggc aagaacagtg gaaaaaggaa caaatcaaat gtaaaactaa
      661 tagagaaaaa ttttatttat ataatgaact atctttaaca accgaatact attatccact
      721 tcaaaacgca ataattgaat tttacactga atattataaa actaatagca ttaatgaaaa
      781 aatgaataaa ttagaaaaca aatatataga tgcttatcat gtaattttta aggagggtaa
      841 tttaaatggc gaatggtgta ttaatgatgt taatgctgtt agtaaaatag cagcaaacgc
      901 tgttaatgga attgttacct ttacacatga acaaaatatt aatgaaagaa ttaaacttat
      961 gaacaagttc tctcaaattt ttttaaatgg acttagtaaa taattatata tatattcttg
     1021 aaataccaat aaatattatt tctaaattac gttcgttttt gacacaaaac ctcttaactt
     1081 aatattaaga ggtttttctt ggatatttaa gtctataaac ttgtgttata atcaatgtat
     1141 cataaatatc gaaaatcatg agtttttaga tgctgacaga cgtgtttatt tacatcgaga
     1201 actatatttc attagtaagt acccataaaa cgattaaaaa aggtggtgtt caaatggcta
     1261 aaattggtta tgcacgtgtg tcaacacaag atcaaagtct tgatggacag attgatacac
     1321 ttgaggaata tggttgtgaa cgtatcttta gcgagaaagc gagtggtcgt aaaactaaaa
     1381 gaacggaact tgataagtgt ttagattatt tacgtgaagg agatattcta gttgtctata
     1441 aattagatcg tcttggacgt acaacaaaac aattaattga gctatctcaa tggttggatg
     1501 aaaatagcat tgatttacat attatagata tgaatgtgtc aacgaaagat gcgatgggca
     1561 agatgttctt taccatgatg agtgcattcg ctgaacttga agctaattta ttaagtgaac
     1621 gaactaaaaa aggtttggaa gctgcaagag caagagggag aaaaggtgga agaccttcac
     1681 taccagatca taagaaaaga gagatcaaat tcttatatga tgaacaaaag ctgtctggtg
     1741 aagaaattgc tgaacaaaca ggagtgagtc gttctactgt atataggatt attaaagagt
     1801 caagaaaaat ataaagtact aaattaaagt tttaatatac cctttaattg aaaaatcttc
     1861 ttaaaaagtg tctttaactt cagtcccctt taacaatcca ctcgcttaat agctttaaat
     1921 ttctaagcat tacttgagct ttgaaaattt tcatcgattc attaaccgac ataactggat
     1981 tttgactgtt aatattcacc ttgtcctctc caattaacaa aaaagcaaaa gcatataaac
     2041 aacatttgtt gtttatatgc ttttgcttgc attttcttct atagtatgct ataatgaaaa
     2101 ggttaagtct atctatacat caaaagcaat aaacttccac ctagaataac aacttattgt
     2161 aaatcgacaa ttacaataat acatcattct atattggaag taaacaaaaa acttacagaa
     2221 ggatgatata gagtgacact agtatgtacc aaattcaatc atgatgctaa atcaactgat
     2281 tatttaaata tttctattga agaagacact cttcaatata tagacccaaa ccttattaaa
     2341 gtaatatcta atagagaagt tggaaaagtt gcgactttgg ctcaaagagc acaagagcaa
     2401 attgatgttt tttttgaaaa aattatcgat atacatctta gtgatttttc tgatacccaa
     2461 aagcgtaatg aatttagaaa attattctct catttttcag aaccacacca tttacgctta
     2521 ggtttttctc agaaaggtaa tacaggaaaa ggtactacag catctgaatt gattaatcta
     2581 tttatgaatg aagatattct ttccattatc ttaaatgatg acggattaac aatacctcaa
     2641 aaagcaccat taatcagaaa ttttggtgat gataaattat cagatttaac aagtaatata
     2701 atcatgaatg ttataattga ttttaataaa tccatgattc agtcatttcc agaaatgaaa
     2761 tcgtttcttt ctaaagaaac tgtgacatat tattatttct ctactaacgg tatatgggaa
     2821 acttatgatt tcactccatt tctttttgat ggtaataaaa ctttattagt tccaaaatta
     2881 ttcactactt ataatcagac atctagttta gatttgatga taagagtata cattgaagaa
     2941 gaaatcatta gattgcaaaa acaacgtaat acaaccaaaa aaatgacaaa aaaacaattt
     3001 atcaatgaat ttataaaagg acatcgtatg gataatatga cctatttctt tttaaacact
     3061 tcttcaaaat cacacaaaaa atttcaaaaa gaattagatt caaaagccaa tgaaaggggt
     3121 aacaataaca aagatgataa ttagtactta ctaatcagta tagtatagac ttaacatact
     3181 aattattcaa ttttgcattc ctccacacta tgatgttata atttagataa caaatatgtg
     3241 ttgaacatgg taaacaccga gcccgataac tattccagta gttatcgggc tctttttggt
     3301 gttattactg tgtcgtcgct ttattgttta tcacgattat tcaaccaatg tgaaaacaac
     3361 gtgattataa cccctactat aataggagct actatagata caaatatgat gctgaacatg
     3421 gtgtcacctc cttccatgct aaaccagcac agaagtagaa cgacgacttt tatattatat
     3481 catttgtgaa ttgttaagca tatgcacttc cgataagtca ttttatgttt aatgtatcta
     3541 tgtgataatg aaaatacaaa ttctattaaa ggagataatt ttatgaaaaa acgatcatta
     3601 cttccaactt tattattggc tagctcatta acattcacag gtttttcagc gacagccaat
     3661 gctgaggatg gtgccaaaac cgtatacgga gagaacttac caaatagcgc agatacaaat
     3721 ggagatggtt gggctaatgt agacttcaat acttcatccc tcccacaatc tgcacaaaat
     3781 caaataagtg aactttctaa acagaaagat tcaggggaat taactcaaat cgagtataat
     3841 gagaaagttt caagtgtatt taatgaatat caatcacaaa gtactgatat acaaaataat
     3901 gagagaactc aacaaggtaa aagacctttc ggaggggtga tacctaattg tatgactcaa
     3961 gaagaatacc aagaattaga aagtcatgtt cctaacccta atagtgtatc cacagaaaaa
     4021 tataatcaaa ttgtaaaaaa tgaaagtcaa aagatagcta atgaagaaaa taaaactatt
     4081 aatacacctc aaggtgtgtt gacacctgta actgaaaata aaaacaagag ttctgaagga
     4141 atgaaaaatc aagattcaaa ctctcaagta ttaccagaaa caggagaaag caaccaaagt
     4201 gatatattgt caataattgg cgcttctgga ctactaatat taggtataag tcttttaata
     4261 ttcagaaaaa aggcaatata aaaattgata aaataaccac cgtgtttatg taaaacatgt
     4321 ggttatttta ttatatattt aatacatatt tttcaactca tgtaatttac gttttcttgc
     4381 tgaaaaaata ttaacataat ctaatttgaa atactgaaaa ggagttgtaa tatgaaaaaa
     4441 atcattttac ctatattaat tgttagttta tcggtaattt tagtgagttg taataataac
     4501 gattcagaac atcacaatga taataattct agtgatacta acacctcaga aaacaaagat
     4561 aatgaagcat caaatgaaat tgacgaaaaa actaacacat ctgatagtgc ggataattca
     4621 gaaagtagta ataaacaaaa taaagaaaaa aatgataata aaaaacaaga aaaaaataat
     4681 gatagtaata agaagcaaca atctactgat aaaaataaaa gttacttaaa taattttaac
     4741 tcgaaagaaa ttgaatatgc acgagtttgg tatcagttaa tatctacaag aaatgattta
     4801 aaaggtatta aaaatgttta tgttacagaa ataccaaaag gctcaaaagt aaatccacaa
     4861 gccaaaaata gtgcaattta taaagaaaaa gttgtgaaat tagaagctcc tatgagagct
     4921 ggtgggtctg taacatatag cagtaatggt gatggaacaa ttaatgtata taataatatc
     4981 ccttataaat gggaaaattc acaaaatagt gattatagtc aaatgaataa gataacaaaa
     5041 aaagcaatag aggagaatat tgaaacagtc tatattaaac cctacgacaa taaaacagta
     5101 gctaagttag caagtaaaat tagatataat aagtagttag tagattatta aaaaggcgcc
     5161 taaaattagg cgtctttttt atcattagtt caaaagataa gcataacgtt ccatgtaaca
     5221 gcatataaag taatgaatca attcatcact atctaatgcc accattgtta gttcagcctt
     5281 gcatggttta acgtatctca cataatggta caaatcttca aatagtgcct gtttttcggg
     5341 cattttaaac accttgtgta cattaaaacc tatttcttct aatcgctttg taatcacgcc
     5401 gaaaacgaat gtgttcatgt gaaatcactc catcattttt aatattcaca tgtacactaa
     5461 aaaaaatccg atacatcggc tttttatttt tggtttaatt tatttctata ttgttttcac
     5521 tatcgctcgg acgcataagt gttaaaacac cttctaccat acctatcaca cccggtattc
     5581 ccgtccaaca aaatagtaaa taaagaagag catttttatt tttcttaaca taaaacttat
     5641 gagccccgaa acctcctaga aaaaatgcta atgcagagta aacatattta ttaacttgca
     5701 tgatttccac tcctttaatt aattgattta gagaaacatt aaataattta gtgatttcta
     5761 aaattgtttt taagtcggga ttacttactt ctctttccca attagaaata gaatgtctcg
     5821 atacccctaa cttttcagct aatttttctt gagacatgtt tttacttttt cttaactttt
     5881 gtatttgatg tccaattttt gtttccaaat tatttttctc cttaacttta agatatattt
     5941 aaactcaatt ttttctatcg atatttatga acattagtat tttttatgct gaaatatgtg
     6001 aacaatcgca taatatcaag gtttttctat aattatacac gttttaaaat acattgtgat
     6061 aatacataga atgtacctta tctaagtata taaatgtttt ctttaaacca aagctaattt
     6121 accacattgg gttaagtttc ttattattaa agaatgaact ttgtatgatt tcaactgctt
     6181 actatcttct catttggtta tttctctctt gtttcttctt ctttttctga tgattaatat
     6241 tgttttgctt ttcagtttcg ttcgcatagt acataagaaa gtcactagca tttatcgttg
     6301 gtaaattaat gtgattagtt tgttcatttt catgttcaat acgattgtca tgatcatttc
     6361 tatctacttg attaatatgt ttttcgataa cttcttcttg atcgacatga ttatatttct
     6421 caagtttaat gagtaattga actttttcat taggtgtttg gtctttgact ttttcaaagt
     6481 ttttatcgcc tatatctatt cttaaatcat catcaatatg ttctttataa ctatcaattt
     6541 ctttttcttt ttccttaata aattgcaact caacgttata ttcaaagcta tctttttcta
     6601 aatttttaag atgtttttga ctaaagaaaa tatctttact cgctttttta taagtatctt
     6661 ctagcaatac atcattagga atattgttat tttgtttgaa caggtattga acatgagttg
     6721 cttttataat attacctttt tgtgcctcat caacaataaa tgctttttca tcatggctaa
     6781 aatgatgatg agatttaggg tacatttgct gaacaatgag atgatttttt tcactttcag
     6841 ttagataaac atctttagct gattgataaa tctctttacg tttttcctct ctactttcta
     6901 atttattctt ctcattgagt aacgctttat agttagtttc aacagtatta acaaactctt
     6961 gttgactata tcctttactt tcaatttctt ttctaaactg ataattttca tcataatttt
     7021 tagcatagaa ttttaattga cgtctcttaa tttcattttt aagtaaatca ttttgaattt
     7081 tacgaccaaa ggtagacgca ctgtcaaacg tatttttaat tgtttctttc gcctctgaat
     7141 agccaacatc acgttgatat ctatcaaata aaatagaata actttgttca tttttactta
     7201 cttgttcgtt agcattttga ataaataatc taatttcatt ttgtgcttgt tctttctctt
     7261 tatcactaaa taattgtgat acttcgccac gattatatct tctaatttgt tcatttttct
     7321 gtccgtaata agtaacgggt tggtactcga taccctcttt ttcacagcgt tttctttcat
     7381 tttgttctaa tctatattct tctcttgtaa gtggaatttc ggcaattttt tctaaacctt
     7441 gcgcttcaaa agaacgacta tcatatcgtc ttaaactttg ttcacgttct gagtattgat
     7501 ttaattttaa ttcccaatgc ttacgaactt ctttgaaatc aaagtcggca aatcgttctt
     7561 gttttctaac tttattacca tttttattta aaatcgcatt gccattttca tcatattgcg
     7621 ttctcgtttt tgtctttttc ccccaagtac cgtctgcatt aaacggtctt tgagtaagta
     7681 gaacatgggc gtgtgggttg tttatatcat ctctatgaat tgaaatatca gcaaccattc
     7741 ctttgtctac aaactcactt tttataaaat ctttagttaa ttcagtttgt acctctctat
     7801 caaaatcatt aggcaaactt aataagactt cttttgctaa tctaggattt tttgttttcg
     7861 cattattctt ttcaacttta tcaacttcat tccataaacg ctgtctatct ttagtccatt
     7921 cgggtgcatg ttgaggagtt aaaatcatgc tttcaggctt aatagtatga tttctaaact
     7981 taattttttc atctgtacgt tcgctatata atgcttcatc agaacgataa gacgctgacg
     8041 caacgatatt ttttgttgct cgattaatgt tagtcactct caacataaag taacctttat
     8101 tcatgatttt acctcctctc ttttctgttt ttttacccct gtgggaacta taatgatcaa
     8161 ccagtatggt tgcccatttt tatgccaatt ttcaatttgc atgtaaatgg gcagtgcagt
     8221 gtcttaagaa ctggttatat tataccatgg atgtgcgact tacccaatga attatggtat
     8281 gctttatata gataaactaa gggagttttt taatgtctaa aattaaacaa acagatatta
     8341 caaaattaag tcaattagaa aaacaaattg cacaactgaa agagcaaaag aaaaaacaag
     8401 aagaacaact atctaataaa attggtggtt attttttaaa taaacttgat ttaaatatga
     8461 tcaactctag tgatgaattg tataaaatta tggataaagt cattggagaa tttaataaag
     8521 atatcgaaca aaatagcagc aacgagaaca aggaaaattt aacagaagac aaaattaact
     8581 agaatagtaa gtgatacgat gaatgatttt aaacatctca aaaaaataaa tgctgaaatt
     8641 gaaaaagcag aattaagaaa gcatagatta aaaaatttag atagaaaaga aagagcacat
     8701 agactgattc gaaaaggtgc aatgcttgaa aagtattttg aatgtgagca tttaagtcct
     8761 gatgaaacag aagaattgtt aaagatgtat tccaactaca ttaatacaaa taaacccaat
     8821 aaatataaaa agaagtaatt tatcatgctt acaaatactt gggtttcact aaaaagaaaa
     8881 aattgctgat agtagtaaca atgaataata attgtttata aaaatgggac taaaaaagaa
     8941 aagttttagt cccacttact taattaatta catttcattg aaaataaagt tcgcttacag
     9001 ttaagagaac atctaattaa agatattctc taattggatc attgttggct tattaagttg
     9061 tattattgat taaataaaga agtgattggt aaatgatcac gtcgtttatt aagcgtatgt
     9121 acttctgcaa attcagattt aagagatttc attaagtacc cttgcattgc ttcaacggtt
     9181 tcattttttt gaattaatgt agctttaaaa cgcttaagaa cattaacaat ttcaaattcc
     9241 atatcttcta ataaataata tgcatcaata gaattgttga aggatgtttt ggcttttaat
     9301 aacacagatt taataagttt aatttcagat acagaaaagt tacttaaata actttgaata
     9361 ctttgtggta gttcttcaag taattgaaat tttaaagctt catcattgaa ttttgaatag
     9421 tagtgatttg aatgatttgt atgattacta gaatatgtta atttcctatc attcaaatca
     9481 tacatatcat tataattagt cttaatataa tcagtgtcat tataatcagt atcattagac
     9541 tgtgaatttg gaagttcttg accacttaaa ttcacagttc tagaacttcc aaattcattg
     9601 ttctggaact gtgaatttgg aagttccttg atatgactgg cttcaatgtc ataattaggt
     9661 tttaataggt ataatctatt tggtttatta aggccaacac gttcttgagt tagtaaatta
     9721 aattttatta actctttttt taattttata attttttgtt ctgcataacc taagtattca
     9781 cacaaatgtt tattactgaa aataaagtaa atattattat ttttatctac ccattcattt
     9841 tggagggata attcaaaacg atcttttaat aacgcataca taattttggc ttctgcagac
     9901 attttcttaa aatatgcatc ttcaaataaa tatttaggca gttgataaaa tctttcagtt
     9961 tttacttgtg ataaattata tttttcaaaa ttgggcataa taaaaacctc atttccattt
    10021 tgggattgag gtaataaaac aatttttacg taagttatat gttttgtgct tgcacttaga
    10081 tgattagata tatataatac aagtatcaaa aaaatatata aacaaacgag tgcgaatcgt
    10141 tttattaccg agagtcgatg ctacgccaat agcatcgact cttttaatat ttataaagta
    10201 atattaacac ttttaatggc gtatataaag agttttaaat gtaattaaaa gtacacctaa
    10261 aactaccaaa cgtataattc gctattttag cacctaatta agtggcgaaa atattttaag
    10321 gatagataat taggcattta ggtagttaat agatagtaaa tttagtacta aatgatgaaa
    10381 taaattttgt taggtggtaa aatttaggta ctaaattagg tacctaaggg gggatgttat
    10441 gaaaactatt aaaatgattg ctgatgaatt aggtgtaact aaacaaacta ttgttaataa
    10501 tgctaaaaga ttaaatatat cttttgaaaa agaaaatgga attaattata ttaaagataa
    10561 tgattattta aaaattatag aaaaaatcac taagaaagag aaaacaactc ataataaaga
    10621 atcagtaaaa aatgaattat ttaatgaaaa tactgcaaaa gttgaaaagt atatacataa
    10681 taattcaaat agttctgaga cattaaaaac aaaagtaaac gaattagaaa agcaaataga
    10741 aatctttgaa actagagcta aaaatgatga aaaatatatt gagaatctaa ctaaacaatt
    10801 agatcaacaa aatagcaatg ttaatacatt aaataaactg ttggagaatc aacaaatatt
    10861 atctttagaa aacaataaaa aaattcaaaa actagaaagc caattagaag aagaaagaca
    10921 gcttaaatat tcttttgata caacgatgaa taatagacag aatattaatc cacaagaagc
    10981 aacttttact gaagaatctc aaaatatcaa ccaacaacaa aaagaatgtc aaccaaccga
    11041 agtaaatcat aaaaatataa ctgaagagaa taagcatgac agtacagatg aattaacaaa
    11101 agaggataag tttgttgaaa aaacgaagaa cgattccagt gataataggg aggtagaacc
    11161 acctaaaaag agattttgga gtcgtttatt tggtaactaa ttgcaattaa aaaggcacac
    11221 aagttcatga acttgttgtg cctttttaat gtgaatcaaa cttctgataa tcatcagtta
    11281 tgttaaactc taacaaaaac taaattatca gaagaattat ctagggtatt aaacataggt
    11341 aaatcaattt tctatcgcca atagtataaa catcatccat attaatctga agatagctac
    11401 ataattcttt aacagcatag cctttatttg tataaggcgc cataaactct attaaatatg
    11461 gtttgttttg aggtttctat gtttaatcat ttaatttttc attatataaa tcgaataatt
    11521 ataaaaaatc atcatacttt ttatttttta aaaatatttg aaaatttaat agtaatccaa
    11581 ttataagatc aaatatcatt atcattataa tcaaggcaac tataatcact gtgacactcg
    11641 ttacataaca agctagtaat ctccagataa aattgacatt ttcaccaatt ccataactta
    11701 acatattaca aagtagtaaa agaataataa tacctatact gtaaattaaa attgtcagtg
    11761 tatatttttt aataactcta ttaggaaaca atagttcatc tattaggttc tttttcacca
    11821 gtatctctcg ttcaattcta tctttgtcac agattttgat tttactattt tttatttttt
    11881 cctctaataa aataacctcg aacgatctgc taatttttaa taaatatgca ggaaaagcta
    11941 atgttaagca taatgtaatt aattcattta cattgttcat gtaacttata aaatagatga
    12001 aagttgctac tagtgctcct aaaaatatag ttatattaag ttctacatca aatattttaa
    12061 aaatatctgc tctattcatc agttaatcat cctttcaagg tttattaata ctaataaatt
    12121 attagatata ggtatatcat attattaatt taagaaattg tcttttataa ttttacttaa
    12181 taataaaaaa gtagaaccat ttaaaattaa tggttctact ttttaactat tactaatttt
    12241 aaaaataaac taatctacaa tatctaaaaa tatatgttta gtactatttt tgtttagtta
    12301 tatcaagtga tttgggaatt aacaaataga caactattga aatgataatc atgataatcc
    12361 cacctactaa agcagttgct acaaaagcat cattaaatga tgttacagcc tcgtttgcaa
    12421 gctgttttat ccccgtagct ttagctactt cgacagcgcc cactacagat tcttcagcta
    12481 catgagctaa atctccaact ataccttttg atgaaaaaga tgaaatatct aaaaatacac
    12541 gataaagcat agaagatagg ctaccaagta ctgctacacc aaaaacattt ccaaggtcat
    12601 acatagactc ttcaacagca gctgcattac ctgcttttga tgtaggtgtt tctaacatta
    12661 ttagagcaga tgcaactgct agtgaagcca taccagctcc aactaaaatt aatgctaaag
    12721 ccattgtaga atatgataat ggatgaccaa agaaatacat aataaacatg ccaatggctg
    12781 caattccaat tccggaaggt aacactattt tcggtccaaa tcgcgccgct aatccgggtg
    12841 caattggtgc aaacaccata tctcctattg ccataggtaa taggtataag ccagctttaa
    12901 aaggagaaag ttcttccaca acctgtaacc attgtgaagc taataacaaa acagatgcca
    12961 ttgcaaaaat tgtcataaat gcagcaattg taccagctga aaatgatctc tttttaaaaa
    13021 gtcttacgtc taacattgga tcagaacttg ataaattacg tttaacaaat atcactatca
    13081 tggtaattgc taatactatt acaacccatg gaataatatc tgctagtcct tcttttgaaa
    13141 attctttgat actccatacc agtccaatca tgcctgcaat tgataaaatt gtagaaggaa
    13201 tatcccacga gtgagacttt tcttttgata acttagactc tggtaataaa aataaacctg
    13261 ctacaactgc tattatcgca aacggtacat taattaaaaa tgccgagtgc catgaaaatt
    13321 gctcaagtaa agctcctccg ataattggtc caaaaacagc acctatcgat gaagcgattg
    13381 accatacagc taatgcagtg gccctttctt tagggttttc aaaaattact cttatcattg
    13441 aaagagtagt tggcattatt aaagcacctg caataccaag taaaaatcga atagctatta
    13501 cgaactctgc actttctgcg aaaaatatag ctaatgaaac gaggccaaat aaagcaaatc
    13561 cagttaataa
//
