LOCUS       NZ_CP073882            13570 bp    DNA     circular CON 17-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1286247 plasmid p1B1286247,
            complete sequence.
ACCESSION   NZ_CP073882
VERSION     NZ_CP073882.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699893
            Assembly: GCF_019330045.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 191x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019330045.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 06:56:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,687
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,525
            CDSs (with protein)               :: 2,525
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 42 of 81
            Pseudo Genes (incomplete)         :: 44 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 24 of 81
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073882.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1286247"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p1B1286247"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(11900..13570,1..252))
                     /locus_tag="KFV53_RS13250"
                     /old_locus_tag="KFV53_13260"
     CDS             complement(join(11900..13570,1..252))
                     /locus_tag="KFV53_RS13250"
                     /old_locus_tag="KFV53_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            461..730
                     /locus_tag="KFV53_RS13255"
                     /old_locus_tag="KFV53_13265"
     CDS             461..730
                     /locus_tag="KFV53_RS13255"
                     /old_locus_tag="KFV53_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            747..986
                     /locus_tag="KFV53_RS13260"
                     /old_locus_tag="KFV53_13270"
     CDS             747..986
                     /locus_tag="KFV53_RS13260"
                     /old_locus_tag="KFV53_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(1211..2146)
                     /locus_tag="KFV53_RS13265"
                     /old_locus_tag="KFV53_13275"
     CDS             complement(1211..2146)
                     /locus_tag="KFV53_RS13265"
                     /old_locus_tag="KFV53_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            2587..3348
                     /locus_tag="KFV53_RS13270"
                     /old_locus_tag="KFV53_13280"
     CDS             2587..3348
                     /locus_tag="KFV53_RS13270"
                     /old_locus_tag="KFV53_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            complement(3667..4227)
                     /locus_tag="KFV53_RS13280"
                     /old_locus_tag="KFV53_13290"
     CDS             complement(3667..4227)
                     /locus_tag="KFV53_RS13280"
                     /old_locus_tag="KFV53_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(4432..5976)
                     /gene="qac"
                     /locus_tag="KFV53_RS13285"
                     /old_locus_tag="KFV53_13295"
     CDS             complement(4432..5976)
                     /gene="qac"
                     /locus_tag="KFV53_RS13285"
                     /old_locus_tag="KFV53_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            6155..6721
                     /gene="qacR"
                     /locus_tag="KFV53_RS13290"
                     /old_locus_tag="KFV53_13300"
     ARG             6156..6718
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6972..7532
                     /locus_tag="KFV53_RS13295"
                     /old_locus_tag="KFV53_13305"
     CDS             6972..7532
                     /locus_tag="KFV53_RS13295"
                     /old_locus_tag="KFV53_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            7951..8862
                     /locus_tag="KFV53_RS13300"
                     /old_locus_tag="KFV53_13310"
     CDS             7951..8862
                     /locus_tag="KFV53_RS13300"
                     /old_locus_tag="KFV53_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(9039..9137)
                     /locus_tag="KFV53_RS13305"
                     /old_locus_tag="KFV53_13315"
     CDS             complement(9039..9137)
                     /locus_tag="KFV53_RS13305"
                     /old_locus_tag="KFV53_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            9301..9999
                     /locus_tag="KFV53_RS13310"
                     /old_locus_tag="KFV53_13320"
     CDS             9301..9999
                     /locus_tag="KFV53_RS13310"
                     /old_locus_tag="KFV53_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            10150..10854
                     /locus_tag="KFV53_RS13315"
                     /old_locus_tag="KFV53_13325"
     CDS             10150..10854
                     /locus_tag="KFV53_RS13315"
                     /old_locus_tag="KFV53_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(10903..11136)
                     /locus_tag="KFV53_RS13320"
                     /old_locus_tag="KFV53_13330"
     CDS             complement(10903..11136)
                     /locus_tag="KFV53_RS13320"
                     /old_locus_tag="KFV53_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(11213..11626)
                     /locus_tag="KFV53_RS13550"
                     /old_locus_tag="KFV53_13335"
     CDS             complement(11213..11626)
                     /locus_tag="KFV53_RS13550"
                     /old_locus_tag="KFV53_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     ARG             complement(4436..5976)
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          complement(1212..2146)
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11970..13570)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 tgttttcgca ttattctttt caactttatc aacttcattc cataaacgct gtctatcttt
       61 agtccattcg ggtgcatgtt gaggagttaa aatcatgctt tcaggcttaa tagtatgatt
      121 tctaaactta attttttcat ctgtacgttc gctatataat gcttcatcag aacgataaga
      181 cgctgacgca acgatatttt ttgttgctcg attaatgtta gtcactctca acataaagta
      241 acctttattc atgattttac ctcctctctt ttctgttttt ttacccctgt gggaactata
      301 atgatcaacc agtatggttg cccattttta tgccaatttt caatttgcat gtaaatgggc
      361 agtgcagtgt cttaagaact ggttatatta taccatggat gtgcgactta cccaatgaat
      421 tatggtatgc tttatataga taaactaagg gagtttttta atgtctaaaa ttaaacaaac
      481 agatattaca aaattaagtc aattagaaaa acaaattgca caactgaaag agcaaaagaa
      541 aaaacaagaa gaacaactat ctaataaaat tggtggttat tttttaaata aacttgattt
      601 aaatatgatc aactctagtg atgaattgta taaaattatg gataaagtca ttggagaatt
      661 taataaagat atcgaacaaa atagcagcaa cgagaacaag gaaaatttaa cagaagacaa
      721 aattaactag aatagtaagt gatacgatga atgattttaa acatctcaaa aaaataaatg
      781 ctgaaattga aaaagcagaa ttaagaaagc atagattaaa aaatttagat agaaaagaaa
      841 gagcacatag actgattcga aaaggtgcaa tgcttgaaaa gtattttgaa tgtgagcatt
      901 taagtcctga tgaaacagaa gaattgttaa agatgtattc caactacatt aatacaaata
      961 aacccaataa atataaaaag aagtaattta tcatgcttac aaatacttgg gtttcactaa
     1021 aaagaaaaaa ttgctgatag tagtaacaat gaataataat tgtttataaa aatgggacta
     1081 aaaaagaaaa gttttagtcc cacttactta attaattaca tttcattgaa aataaagttc
     1141 gcttacagtt aagagaacat ctaattaaag atattctcta attggatcat tgttggctta
     1201 ttaagttgta ttattgatta aataaagaag tgattggtaa atgatcacgt cgtttattaa
     1261 gcgtatgtac ttctgcaaat tcagatttaa gagatttcat taagtaccct tgcattgctt
     1321 caacggtttc atttttttga attaatgtag ctttaaaacg cttaagaaca ttaacaattt
     1381 caaattccat atcttctaat aaataatatg catcaataga attgttgaag gatgttttgg
     1441 cttttaataa cacagattta ataagtttaa tttcagatac agaaaagtta cttaaataac
     1501 tttgaatact ttgtggtagt tcttcaagta attgaaattt taaagcttca tcattgaatt
     1561 ttgaatagta gtgatttgaa tgatttgtat gattactaga atatgttaat ttcctatcat
     1621 tcaaatcata catatcatta taattagtct taatataatc agtgtcatta taatcagtat
     1681 cattagactg tgaatttgga agttcttgac cacttaaatt cacagttcta gaacttccaa
     1741 attcattgtt ctggaactgt gaatttggaa gttccttgat atgactggct tcaatgtcat
     1801 aattaggttt taataggtat aatctatttg gtttattaag gccaacacgt tcttgagtta
     1861 gtaaattaaa ttttattaac tcttttttta attttataat tttttgttct gcataaccta
     1921 agtattcaca caaatgttta ttactgaaaa taaagtaaat attattattt ttatctaccc
     1981 attcattttg gagggataat tcaaaacgat cttttaataa cgcatacata attttggctt
     2041 ctgcagacat tttcttaaaa tatgcatctt caaataaata tttaggcagt tgataaaatc
     2101 tttcagtttt tacttgtgat aaattatatt tttcaaaatt gggcataata aaaacctcat
     2161 ttccattttg ggattgaggt aataaaacaa tttttacgta agttatatgt tttgtgcttg
     2221 cacttagatg attagatata tataatacaa gtatcaaaaa aatatataaa caaacgagtg
     2281 cgaatcgttt tattaccgag agtcgatgct acgccaatag catcgactct tttaatattt
     2341 ataaagtaat attaacactt ttaatggcgt atataaagag ttttaaatgt aattaaaagt
     2401 acacctaaaa ctaccaaacg tataattcgc tattttagca cctaattaag tggcgaaaat
     2461 attttaagga tagataatta ggcatttagg tagttaatag atagtaaatt tagtactaaa
     2521 tgatgaaata aattttgtta ggtggtaaaa tttaggtact aaattaggta cctaaggggg
     2581 gatgttatga aaactattaa aatgattgct gatgaattag gtgtaactaa acaaactatt
     2641 gttaataatg ctaaaagatt aaatatatct tttgaaaaag aaaatggaat taattatatt
     2701 aaagataatg attatttaaa aattatagaa aaaatcacta agaaagagaa aacaactcat
     2761 aataaagaat cagtaaaaaa tgaattattt aatgaaaata ctgcaaaagt tgaaaagtat
     2821 atacataata attcaaatag ttctgagaca ttaaaaacaa aagtaaacga attagaaaag
     2881 caaatagaaa tctttgaaac tagagctaaa aatgatgaaa aatatattga gaatctaact
     2941 aaacaattag atcaacaaaa tagcaatgtt aatacattaa ataaactgtt ggagaatcaa
     3001 caaatattat ctttagaaaa caataaaaaa attcaaaaac tagaaagcca attagaagaa
     3061 gaaagacagc ttaaatattc ttttgataca acgatgaata atagacagaa tattaatcca
     3121 caagaagcaa cttttactga agaatctcaa aatatcaacc aacaacaaaa agaatgtcaa
     3181 ccaaccgaag taaatcataa aaatataact gaagagaata agcatgacag tacagatgaa
     3241 ttaacaaaag aggataagtt tgttgaaaaa acgaagaacg attccagtga taatagggag
     3301 gtagaaccac ctaaaaagag attttggagt cgtttatttg gtaactaatt gcaattaaaa
     3361 aggcacacaa gttcatgaac ttgttgtgcc tttttaatgt gaatcaaact tctgataatc
     3421 atcagttatg ttaaactcta acaaaaacta aattatcaga agaattatct agggtattaa
     3481 acataggtaa atcaattttc tatcgccaat agtataaaca tcatccatat taatctgaag
     3541 atagctacat aattctttaa cagcatagcc tttatttgta taaggcgcca taaactctat
     3601 taaatatggt ttgttttgag gtttctatgt ttaatcattt aatttttcat tatataaatc
     3661 gaataattat aaaaaatcat catacttttt attttttaaa aatatttgaa aatttaatag
     3721 taatccaatt ataagatcaa atatcattat cattataatc aaggcaacta taatcactgt
     3781 gacactcgtt acataacaag ctagtaatct ccagataaaa ttgacatttt caccaattcc
     3841 ataacttaac atattacaaa gtagtaaaag aataataata cctatactgt aaattaaaat
     3901 tgtcagtgta tattttttaa taactctatt aggaaacaat agttcatcta ttaggttctt
     3961 tttcaccagt atctctcgtt caattctatc tttgtcacag attttgattt tactattttt
     4021 tattttttcc tctaataaaa taacctcgaa cgatctgcta atttttaata aatatgcagg
     4081 aaaagctaat gttaagcata atgtaattaa ttcatttaca ttgttcatgt aacttataaa
     4141 atagatgaaa gttgctacta gtgctcctaa aaatatagtt atattaagtt ctacatcaaa
     4201 tattttaaaa atatctgctc tattcatcag ttaatcatcc tttcaaggtt tattaatact
     4261 aataaattat tagatatagg tatatcatat tattaattta agaaattgtc ttttataatt
     4321 ttacttaata ataaaaaagt agaaccattt aaaattaatg gttctacttt ttaactatta
     4381 ctaattttaa aaataaacta atctacaata tctaaaaata tatgtttagt actatttttg
     4441 tttagttata tcaagtgatt tgggaattaa caaatagaca actattgaaa tgataatcat
     4501 gataatccca cctactaaag cagttgctac aaaagcatca ttaaatgatg ttacagcctc
     4561 gtttgcaagc tgttttatcc ccgtagcttt agctacttcg acagcgccca ctacagattc
     4621 ttcagctaca tgagctaaat ctccaactat accttttgat gaaaaagatg aaatatctaa
     4681 aaatacacga taaagcatag aagataggct accaagtact gctacaccaa aaacatttcc
     4741 aaggtcatac atagactctt caacagcagc tgcattacct gcttttgatg taggtgtttc
     4801 taacattatt agagcagatg caactgctag tgaagccata ccagctccaa ctaaaattaa
     4861 tgctaaagcc attgtagaat atgataatgg atgaccaaag aaatacataa taaacatgcc
     4921 aatggctgca attccaattc cggaaggtaa cactattttc ggtccaaatc gcgccgctaa
     4981 tccgggtgca attggtgcaa acaccatatc tcctattgcc ataggtaata ggtataagcc
     5041 agctttaaaa ggagaaagtt cttccacaac ctgtaaccat tgtgaagcta ataacaaaac
     5101 agatgccatt gcaaaaattg tcataaatgc agcaattgta ccagctgaaa atgatctctt
     5161 tttaaaaagt cttacgtcta acattggatc agaacttgat aaattacgtt taacaaatat
     5221 cactatcatg gtaattgcta atactattac aacccatgga ataatatctg ctagtccttc
     5281 ttttgaaaat tctttgatac tccataccag tccaatcatg cctgcaattg ataaaattgt
     5341 agaaggaata tcccacgagt gagacttttc ttttgataac ttagactctg gtaataaaaa
     5401 taaacctgct acaactgcta ttatcgcaaa cggtacatta attaaaaatg ccgagtgcca
     5461 tgaaaattgc tcaagtaaag ctcctccgat aattggtcca aaaacagcac ctatcgatga
     5521 agcgattgac catacagcta atgcagtggc cctttcttta gggttttcaa aaattactct
     5581 tatcattgaa agagtagttg gcattattaa agcacctgca ataccaagta aaaatcgaat
     5641 agctattacg aactctgcac tttctgcgaa aaatatagct aatgaaacga ggccaaataa
     5701 agcaaatcca gttaataatg ctttttttct tccccattta tcagcaaagg cactcaatgg
     5761 aattataaag ccagctaaaa caagagagta tatatcaact atccataact gttgggtacc
     5821 agaaggctct aactctctta ctaattccgg taaagccata attaatattg tcatatccat
     5881 tgtaacaaca aacaaactaa cagctaatac tactagtgca gtccatcttt tttttgatgt
     5941 catcatatca gtagtttttg taaaaaatga aatcatttaa gttccctcca atccttatag
     6001 accgtgcgat cggtctataa ggattataat ctattttttt tacatattac aacaaaaaat
     6061 cttgcgaaat ttaaatggct gtattgtgtt atacaattat tctttgaatt ttttgtgcta
     6121 tcattgatag tacttaatac tcattaaagg cgtgatgaac ttgaaagata aaatactagg
     6181 tgtcgcaaag gaattattta taaaaaatgg atataatgcc actactactg gagaaattgt
     6241 taaattatca gaaagtagta aagggaatct ttattatcac tttaaaacaa aagaaaatct
     6301 atttttagaa attttaaata tagaagaatc taaatggcaa gaacagtgga aaaaggaaca
     6361 aatcaaatgt aaaactaata gagaaaaatt ttatttatat aatgaactat ctttaacaac
     6421 cgaatactat tatccacttc aaaacgcaat aattgaattt tacactgaat attataaaac
     6481 taatagcatt aatgaaaaaa tgaataaatt agaaaacaaa tatatagatg cttatcatgt
     6541 aatttttaag gagggtaatt taaatggcga atggtgtatt aatgatgtta atgctgttag
     6601 taaaatagca gcaaacgctg ttaatggaat tgttaccttt acacatgaac aaaatattaa
     6661 tgaaagaatt aaacttatga acaagttctc tcaaattttt ttaaatggac ttagtaaata
     6721 attatatata tattcttgaa ataccaataa atattatttc taaattacgt tcgtttttga
     6781 cacaaaacct cttaacttaa tattaagagg tttttcttgg atatttaagt ctataaactt
     6841 gtgttataat caatgtatca taaatatcga aaatcatgag tttttagatg ctgacagacg
     6901 tgtttattta catcgagaac tatatttcat tagtaagtac ccataaaacg attaaaaaag
     6961 gtggtgttca aatggctaaa attggttatg cacgtgtgtc aacacaagat caaagtcttg
     7021 atggacagat tgatacactt gaggaatatg gttgtgaacg tatctttagc gagaaagcga
     7081 gtggtcgtaa aactaaaaga acggaacttg ataagtgttt agattattta cgtgaaggag
     7141 atattctagt tgtctataaa ttagatcgtc ttggacgtac aacaaaacaa ttaattgagc
     7201 tatctcaatg gttggatgaa aatagcattg atttacatat tatagatatg aatgtgtcaa
     7261 cgaaagatgc gatgggcaag atgttcttta ccatgatgag tgcattcgct gaacttgaag
     7321 ctaatttatt aagtgaacga actaaaaaag gtttggaagc tgcaagagca agagggagaa
     7381 aaggtggaag accttcacta ccagatcata agaaaagaga gatcaaattc ttatatgatg
     7441 aacaaaagct gtctggtgaa gaaattgctg aacaaacagg agtgagtcgt tctactgtat
     7501 ataggattat taaagagtca agaaaaatat aaagtactaa attaaagttt taatataccc
     7561 tttaattgaa aaatcttctt aaaaagtgtc tttaacttca gtccccttta acaatccact
     7621 cgcttaatag ctttaaattt ctaagcatta cttgagcttt gaaaattttc atcgattcat
     7681 taaccgacat aactggattt tgactgttaa tattcacctt gtcctctcca attaacaaaa
     7741 aagcaaaagc atataaacaa catttgttgt ttatatgctt ttgcttgcat tttcttctat
     7801 agtatgctat aatgaaaagg ttaagtctat ctatacatca aaagcaataa acttccacct
     7861 agaataacaa cttattgtaa atcgacaatt acaataatac atcattctat attggaagta
     7921 aacaaaaaac ttacagaagg atgatataga gtgacactag tatgtaccaa attcaatcat
     7981 gatgctaaat caactgatta tttaaatatt tctattgaag aagacactct tcaatatata
     8041 gacccaaacc ttattaaagt aatatctaat agagaagttg gaaaagttgc gactttggct
     8101 caaagagcac aagagcaaat tgatgttttt tttgaaaaaa ttatcgatat acatcttagt
     8161 gatttttctg atacccaaaa gcgtaatgaa tttagaaaat tattctctca tttttcagaa
     8221 ccacaccatt tacgcttagg tttttctcag aaaggtaata caggaaaagg tactacagca
     8281 tctgaattga ttaatctatt tatgaatgaa gatattcttt ccattatctt aaatgatgac
     8341 ggattaacaa tacctcaaaa agcaccatta atcagaaatt ttggtgatga taaattatca
     8401 gatttaacaa gtaatataat catgaatgtt ataattgatt ttaataaatc catgattcag
     8461 tcatttccag aaatgaaatc gtttctttct aaagaaactg tgacatatta ttatttctct
     8521 actaacggta tatgggaaac ttatgatttc actccatttc tttttgatgg taataaaact
     8581 ttattagttc caaaattatt cactacttat aatcagacat ctagtttaga tttgatgata
     8641 agagtataca ttgaagaaga aatcattaga ttgcaaaaac aacgtaatac aaccaaaaaa
     8701 atgacaaaaa aacaatttat caatgaattt ataaaaggac atcgtatgga taatatgacc
     8761 tatttctttt taaacacttc ttcaaaatca cacaaaaaat ttcaaaaaga attagattca
     8821 aaagccaatg aaaggggtaa caataacaaa gatgataatt agtacttact aatcagtata
     8881 gtatagactt aacatactaa ttattcaatt ttgcattcct ccacactatg atgttataat
     8941 ttagataaca aatatgtgtt gaacatggta aacaccgagc ccgataacta ttccagtagt
     9001 tatcgggctc tttttggtgt tattactgtg tcgtcgcttt attgtttatc acgattattc
     9061 aaccaatgtg aaaacaacgt gattataacc cctactataa taggagctac tatagataca
     9121 aatatgatgc tgaacatggt gtcacctcct tccatgctaa accagcacag aagtagaacg
     9181 acgactttta tattatatca tttgtgaatt gttaagcata tgcacttccg ataagtcatt
     9241 ttatgtttaa tgtatctatg tgataatgaa aatacaaatt ctattaaagg agataatttt
     9301 atgaaaaaac gatcattact tccaacttta ttattggcta gctcattaac attcacaggt
     9361 ttttcagcga cagccaatgc tgaggatggt gccaaaaccg tatacggaga gaacttacca
     9421 aatagcgcag atacaaatgg agatggttgg gctaatgtag acttcaatac ttcatccctc
     9481 ccacaatctg cacaaaatca aataagtgaa ctttctaaac agaaagattc aggggaatta
     9541 actcaaatcg agtataatga gaaagtttca agtgtattta atgaatatca atcacaaagt
     9601 actgatatac aaaataatga gagaactcaa caaggtaaaa gacctttcgg aggggtgata
     9661 cctaattgta tgactcaaga agaataccaa gaattagaaa gtcatgttcc taaccctaat
     9721 agtgtatcca cagaaaaata taatcaaatt gtaaaaaatg aaagtcaaaa gatagctaat
     9781 gaagaaaata aaactattaa tacacctcaa ggtgtgttga cacctgtaac tgaaaataaa
     9841 aacaagagtt ctgaaggaat gaaaaatcaa gattcaaact ctcaagtatt accagaaaca
     9901 ggagaaagca accaaagtga tatattgtca ataattggcg cttctggact actaatatta
     9961 ggtataagtc ttttaatatt cagaaaaaag gcaatataaa aattgataaa ataaccaccg
    10021 tgtttatgta aaacatgtgg ttattttatt atatatttaa tacatatttt tcaactcatg
    10081 taatttacgt tttcttgctg aaaaaatatt aacataatct aatttgaaat actgaaaagg
    10141 agttgtaata tgaaaaaaat cattttacct atattaattg ttagtttatc ggtaatttta
    10201 gtgagttgta ataataacga ttcagaacat cacaatgata ataattctag tgatactaac
    10261 acctcagaaa acaaagataa tgaagcatca aatgaaattg acgaaaaaac taacacatct
    10321 gatagtgcgg ataattcaga aagtagtaat aaacaaaata aagaaaaaaa tgataataaa
    10381 aaacaagaaa aaaataatga tagtaataag aagcaacaat ctactgataa aaataaaagt
    10441 tacttaaata attttaactc gaaagaaatt gaatatgcac gagtttggta tcagttaata
    10501 tctacaagaa atgatttaaa aggtattaaa aatgtttatg ttacagaaat accaaaaggc
    10561 tcaaaagtaa atccacaagc caaaaatagt gcaatttata aagaaaaagt tgtgaaatta
    10621 gaagctccta tgagagctgg tgggtctgta acatatagca gtaatggtga tggaacaatt
    10681 aatgtatata ataatatccc ttataaatgg gaaaattcac aaaatagtga ttatagtcaa
    10741 atgaataaga taacaaaaaa agcaatagag gagaatattg aaacagtcta tattaaaccc
    10801 tacgacaata aaacagtagc taagttagca agtaaaatta gatataataa gtagttagta
    10861 gattattaaa aaggcgccta aaattaggcg tcttttttat cattagttca aaagataagc
    10921 ataacgttcc atgtaacagc atataaagta atgaatcaat tcatcactat ctaatgccac
    10981 cattgttagt tcagccttgc atggtttaac gtatctcaca taatggtaca aatcttcaaa
    11041 tagtgcctgt ttttcgggca ttttaaacac cttgtgtaca ttaaaaccta tttcttctaa
    11101 tcgctttgta atcacgccga aaacgaatgt gttcatgtga aatcactcca tcatttttaa
    11161 tattcacatg tacactaaaa aaaatccgat acatcggctt tttatttttg gtttaattta
    11221 tttctatatt gttttcacta tcgctcggac gcataagtgt taaaacacct tctaccatac
    11281 ctatcacacc cggtattccc gtccaacaaa atagtaaata aagaagagca tttttatttt
    11341 tcttaacata aaacttatga gccccgaaac ctcctagaaa aaatgctaat gcagagtaaa
    11401 catatttatt aacttgcatg atttccactc ctttaattaa ttgatttaga gaaacattaa
    11461 ataatttagt gatttctaaa attgttttta agtcgggatt acttacttct ctttcccaat
    11521 tagaaataga atgtctcgat acccctaact tttcagctaa tttttcttga gacatgtttt
    11581 tactttttct taacttttgt atttgatgtc caatttttgt ttccaaatta tttttctcct
    11641 taactttaag atatatttaa actcaatttt ttctatcgat atttatgaac attagtattt
    11701 tttatgctga aatatgtgaa caatcgcata atatcaaggt ttttctataa ttatacacgt
    11761 tttaaaatac attgtgataa tacatagaat gtaccttatc taagtatata aatgttttct
    11821 ttaaaccaaa gctaatttac cacattgggt taagtttctt attattaaag aatgaacttt
    11881 gtatgatttc aactgcttac tatcttctca tttggttatt tctctcttgt ttcttcttct
    11941 ttttctgatg attaatattg ttttgctttt cagtttcgtt cgcatagtac ataagaaagt
    12001 cactagcatt tatcgttggt aaattaatgt gattagtttg ttcattttca tgttcaatac
    12061 gattgtcatg atcatttcta tctacttgat taatatgttt ttcgataact tcttcttgat
    12121 cgacatgatt atatttctca agtttaatga gtaattgaac tttttcatta ggtgtttggt
    12181 ctttgacttt ttcaaagttt ttatcgccta tatctattct taaatcatca tcaatatgtt
    12241 ctttataact atcaatttct ttttcttttt ccttaataaa ttgcaactca acgttatatt
    12301 caaagctatc tttttctaaa tttttaagat gtttttgact aaagaaaata tctttactcg
    12361 cttttttata agtatcttct agcaatacat cattaggaat attgttattt tgtttgaaca
    12421 ggtattgaac atgagttgct tttataatat tacctttttg tgcctcatca acaataaatg
    12481 ctttttcatc atggctaaaa tgatgatgag atttagggta catttgctga acaatgagat
    12541 gatttttttc actttcagtt agataaacat ctttagctga ttgataaatc tctttacgtt
    12601 tttcctctct actttctaat ttattcttct cattgagtaa cgctttatag ttagtttcaa
    12661 cagtattaac aaactcttgt tgactatatc ctttactttc aatttctttt ctaaactgat
    12721 aattttcatc ataattttta gcatagaatt ttaattgacg tctcttaatt tcatttttaa
    12781 gtaaatcatt ttgaatttta cgaccaaagg tagacgcact gtcaaacgta tttttaattg
    12841 tttctttcgc ctctgaatag ccaacatcac gttgatatct atcaaataaa atagaataac
    12901 tttgttcatt tttacttact tgttcgttag cattttgaat aaataatcta atttcatttt
    12961 gtgcttgttc tttctcttta tcactaaata attgtgatac ttcgccacga ttatatcttc
    13021 taatttgttc atttttctgt ccgtaataag taacgggttg gtactcgata ccctcttttt
    13081 cacagcgttt tctttcattt tgttctaatc tatattcttc tcttgtaagt ggaatttcgg
    13141 caattttttc taaaccttgc gcttcaaaag aacgactatc atatcgtctt aaactttgtt
    13201 cacgttctga gtattgattt aattttaatt cccaatgctt acgaacttct ttgaaatcaa
    13261 agtcggcaaa tcgttcttgt tttctaactt tattaccatt tttatttaaa atcgcattgc
    13321 cattttcatc atattgcgtt ctcgtttttg tctttttccc ccaagtaccg tctgcattaa
    13381 acggtctttg agtaagtaga acatgggcgt gtgggttgtt tatatcatct ctatgaattg
    13441 aaatatcagc aaccattcct ttgtctacaa actcactttt tataaaatct ttagttaatt
    13501 cagtttgtac ctctctatca aaatcattag gcaaacttaa taagacttct tttgctaatc
    13561 taggattttt
//
