LOCUS       NZ_CP073879            13570 bp    DNA     circular CON 17-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1285228 plasmid pB1285228,
            complete sequence.
ACCESSION   NZ_CP073879
VERSION     NZ_CP073879.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699892
            Assembly: GCF_019787665.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 578x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019787665.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 07:01:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,544
            CDSs (with protein)               :: 2,544
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 41 of 79
            Pseudo Genes (incomplete)         :: 44 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 24 of 79
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073879.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1285228"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="pB1285228"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(13549..13570,1..248))
                     /locus_tag="KFV29_RS13340"
     CDS             complement(join(13549..13570,1..248))
                     /locus_tag="KFV29_RS13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            457..2379
                     /locus_tag="KFV29_RS13345"
                     /old_locus_tag="KFV29_13360"
     CDS             457..2379
                     /locus_tag="KFV29_RS13345"
                     /old_locus_tag="KFV29_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            2653..3066
                     /locus_tag="KFV29_RS13650"
                     /old_locus_tag="KFV29_13365"
     CDS             2653..3066
                     /locus_tag="KFV29_RS13650"
                     /old_locus_tag="KFV29_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            3143..3376
                     /locus_tag="KFV29_RS13355"
                     /old_locus_tag="KFV29_13370"
     CDS             3143..3376
                     /locus_tag="KFV29_RS13355"
                     /old_locus_tag="KFV29_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(3425..4129)
                     /locus_tag="KFV29_RS13360"
                     /old_locus_tag="KFV29_13375"
     CDS             complement(3425..4129)
                     /locus_tag="KFV29_RS13360"
                     /old_locus_tag="KFV29_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(4280..4978)
                     /locus_tag="KFV29_RS13365"
                     /old_locus_tag="KFV29_13380"
     CDS             complement(4280..4978)
                     /locus_tag="KFV29_RS13365"
                     /old_locus_tag="KFV29_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            5142..5240
                     /locus_tag="KFV29_RS13370"
                     /old_locus_tag="KFV29_13385"
     CDS             5142..5240
                     /locus_tag="KFV29_RS13370"
                     /old_locus_tag="KFV29_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(5417..6328)
                     /locus_tag="KFV29_RS13375"
                     /old_locus_tag="KFV29_13390"
     CDS             complement(5417..6328)
                     /locus_tag="KFV29_RS13375"
                     /old_locus_tag="KFV29_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(6747..7307)
                     /locus_tag="KFV29_RS13380"
                     /old_locus_tag="KFV29_13395"
     CDS             complement(6747..7307)
                     /locus_tag="KFV29_RS13380"
                     /old_locus_tag="KFV29_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            complement(7558..8124)
                     /gene="qacR"
                     /locus_tag="KFV29_RS13385"
                     /old_locus_tag="KFV29_13400"
     ARG             complement(7562..8124)
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            8303..9847
                     /gene="qac"
                     /locus_tag="KFV29_RS13390"
                     /old_locus_tag="KFV29_13405"
     CDS             8303..9847
                     /gene="qac"
                     /locus_tag="KFV29_RS13390"
                     /old_locus_tag="KFV29_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            10052..10612
                     /locus_tag="KFV29_RS13395"
                     /old_locus_tag="KFV29_13410"
     CDS             10052..10612
                     /locus_tag="KFV29_RS13395"
                     /old_locus_tag="KFV29_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(10931..11692)
                     /locus_tag="KFV29_RS13405"
                     /old_locus_tag="KFV29_13420"
     CDS             complement(10931..11692)
                     /locus_tag="KFV29_RS13405"
                     /old_locus_tag="KFV29_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            12133..13068
                     /locus_tag="KFV29_RS13410"
                     /old_locus_tag="KFV29_13425"
     CDS             12133..13068
                     /locus_tag="KFV29_RS13410"
                     /old_locus_tag="KFV29_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            complement(13293..13532)
                     /locus_tag="KFV29_RS13415"
                     /old_locus_tag="KFV29_13430"
     CDS             complement(13293..13532)
                     /locus_tag="KFV29_RS13415"
                     /old_locus_tag="KFV29_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     ARG             8304..9844
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          12134..13068
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          458..2310
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 aaattttcct tgttctcgtt gctgctattt tgttcgatat ctttattaaa ttctccaatg
       61 actttatcca taattttata caattcatca ctagagttga tcatatttaa atcaagttta
      121 tttaaaaaat aaccaccaat tttattagat agttgttctt cttgtttttt cttttgctct
      181 ttcagttgtg caatttgttt ttctaattga cttaattttg taatatctgt ttgtttaatt
      241 ttagacatta aaaaactccc ttagtttatc tatataaagc ataccataat tcattgggta
      301 agtcgcacat ccatggtata atataaccag ttcttaagac actgcactgc ccatttacat
      361 gcaaattgaa aattggcata aaaatgggca accatactgg ttgatcatta tagttcccac
      421 aggggtaaaa aaacagaaaa gagaggaggt aaaatcatga ataaaggtta ctttatgttg
      481 agagtgacta acattaatcg agcaacaaaa aatatcgttg cgtcagcgtc ttatcgttct
      541 gatgaagcat tatatagcga acgtacagat gaaaaaatta agtttagaaa tcatactatt
      601 aagcctgaaa gcatgatttt aactcctcaa catgcacccg aatggactaa agatagacag
      661 cgtttatgga atgaagttga taaagttgaa aagaataatg cgaaaacaaa aaatcctaga
      721 ttagcaaaag aagtcttatt aagtttgcct aatgattttg atagagaggt acaaactgaa
      781 ttaactaaag attttataaa aagtgagttt gtagacaaag gaatggttgc tgatatttca
      841 attcatagag atgatataaa caacccacac gcccatgttc tacttactca aagaccgttt
      901 aatgcagacg gtacttgggg gaaaaagaca aaaacgagaa cgcaatatga tgaaaatggc
      961 aatgcgattt taaataaaaa tggtaataaa gttagaaaac aagaacgatt tgccgacttt
     1021 gatttcaaag aagttcgtaa gcattgggaa ttaaaattaa atcaatactc agaacgtgaa
     1081 caaagtttaa gacgatatga tagtcgttct tttgaagcgc aaggtttaga aaaaattgcc
     1141 gaaattccac ttacaagaga agaatataga ttagaacaaa atgaaagaaa acgctgtgaa
     1201 aaagagggta tcgagtacca acccgttact tattacggac agaaaaatga acaaattaga
     1261 agatataatc gtggcgaagt atcacaatta tttagtgata aagagaaaga acaagcacaa
     1321 aatgaaatta gattatttat tcaaaatgct aacgaacaag taagtaaaaa tgaacaaagt
     1381 tattctattt tatttgatag atatcaacgt gatgttggct attcagaggc gaaagaaaca
     1441 attaaaaata cgtttgacag tgcgtctacc tttggtcgta aaattcaaaa tgatttactt
     1501 aaaaatgaaa ttaagagacg tcaattaaaa ttctatgcta aaaattatga tgaaaattat
     1561 cagtttagaa aagaaattga aagtaaagga tatagtcaac aagagtttgt taatactgtt
     1621 gaaactaact ataaagcgtt actcaatgag aagaataaat tagaaagtag agaggaaaaa
     1681 cgtaaagaga tttatcaatc agctaaagat gtttatctaa ctgaaagtga aaaaaatcat
     1741 ctcattgttc agcaaatgta ccctaaatct catcatcatt ttagccatga tgaaaaagca
     1801 tttattgttg atgaggcaca aaaaggtaat attataaaag caactcatgt tcaatacctg
     1861 ttcaaacaaa ataacaatat tcctaatgat gtattgctag aagatactta taaaaaagcg
     1921 agtaaagata ttttctttag tcaaaaacat cttaaaaatt tagaaaaaga tagctttgaa
     1981 tataacgttg agttgcaatt tattaaggaa aaagaaaaag aaattgatag ttataaagaa
     2041 catattgatg atgatttaag aatagatata ggcgataaaa actttgaaaa agtcaaagac
     2101 caaacaccta atgaaaaagt tcaattactc attaaacttg agaaatataa tcatgtcgat
     2161 caagaagaag ttatcgaaaa acatattaat caagtagata gaaatgatca tgacaatcgt
     2221 attgaacatg aaaatgaaca aactaatcac attaatttac caacgataaa tgctagtgac
     2281 tttcttatgt actatgcgaa cgaaactgaa aagcaaaaca atattaatca tcagaaaaag
     2341 aagaagaaac aagagagaaa taaccaaatg agaagatagt aagcagttga aatcatacaa
     2401 agttcattct ttaataataa gaaacttaac ccaatgtggt aaattagctt tggtttaaag
     2461 aaaacattta tatacttaga taaggtacat tctatgtatt atcacaatgt attttaaaac
     2521 gtgtataatt atagaaaaac cttgatatta tgcgattgtt cacatatttc agcataaaaa
     2581 atactaatgt tcataaatat cgatagaaaa aattgagttt aaatatatct taaagttaag
     2641 gagaaaaata atttggaaac aaaaattgga catcaaatac aaaagttaag aaaaagtaaa
     2701 aacatgtctc aagaaaaatt agctgaaaag ttaggggtat cgagacattc tatttctaat
     2761 tgggaaagag aagtaagtaa tcccgactta aaaacaattt tagaaatcac taaattattt
     2821 aatgtttctc taaatcaatt aattaaagga gtggaaatca tgcaagttaa taaatatgtt
     2881 tactctgcat tagcattttt tctaggaggt ttcggggctc ataagtttta tgttaagaaa
     2941 aataaaaatg ctcttcttta tttactattt tgttggacgg gaataccggg tgtgataggt
     3001 atggtagaag gtgttttaac acttatgcgt ccgagcgata gtgaaaacaa tatagaaata
     3061 aattaaacca aaaataaaaa gccgatgtat cggatttttt ttagtgtaca tgtgaatatt
     3121 aaaaatgatg gagtgatttc acatgaacac attcgttttc ggcgtgatta caaagcgatt
     3181 agaagaaata ggttttaatg tacacaaggt gtttaaaatg cccgaaaaac aggcactatt
     3241 tgaagatttg taccattatg tgagatacgt taaaccatgc aaggctgaac taacaatggt
     3301 ggcattagat agtgatgaat tgattcatta ctttatatgc tgttacatgg aacgttatgc
     3361 ttatcttttg aactaatgat aaaaaagacg cctaatttta ggcgcctttt taataatcta
     3421 ctaactactt attatatcta attttacttg ctaacttagc tactgtttta ttgtcgtagg
     3481 gtttaatata gactgtttca atattctcct ctattgcttt ttttgttatc ttattcattt
     3541 gactataatc actattttgt gaattttccc atttataagg gatattatta tatacattaa
     3601 ttgttccatc accattactg ctatatgtta cagacccacc agctctcata ggagcttcta
     3661 atttcacaac tttttcttta taaattgcac tatttttggc ttgtggattt acttttgagc
     3721 cttttggtat ttctgtaaca taaacatttt taataccttt taaatcattt cttgtagata
     3781 ttaactgata ccaaactcgt gcatattcaa tttctttcga gttaaaatta tttaagtaac
     3841 ttttattttt atcagtagat tgttgcttct tattactatc attatttttt tcttgttttt
     3901 tattatcatt tttttcttta ttttgtttat tactactttc tgaattatcc gcactatcag
     3961 atgtgttagt tttttcgtca atttcatttg atgcttcatt atctttgttt tctgaggtgt
     4021 tagtatcact agaattatta tcattgtgat gttctgaatc gttattatta caactcacta
     4081 aaattaccga taaactaaca attaatatag gtaaaatgat ttttttcata ttacaactcc
     4141 ttttcagtat ttcaaattag attatgttaa tattttttca gcaagaaaac gtaaattaca
     4201 tgagttgaaa aatatgtatt aaatatataa taaaataacc acatgtttta cataaacacg
     4261 gtggttattt tatcaatttt tatattgcct tttttctgaa tattaaaaga cttataccta
     4321 atattagtag tccagaagcg ccaattattg acaatatatc actttggttg ctttctcctg
     4381 tttctggtaa tacttgagag tttgaatctt gatttttcat tccttcagaa ctcttgtttt
     4441 tattttcagt tacaggtgtc aacacacctt gaggtgtatt aatagtttta ttttcttcat
     4501 tagctatctt ttgactttca ttttttacaa tttgattata tttttctgtg gatacactat
     4561 tagggttagg aacatgactt tctaattctt ggtattcttc ttgagtcata caattaggta
     4621 tcacccctcc gaaaggtctt ttaccttgtt gagttctctc attattttgt atatcagtac
     4681 tttgtgattg atattcatta aatacacttg aaactttctc attatactcg atttgagtta
     4741 attcccctga atctttctgt ttagaaagtt cacttatttg attttgtgca gattgtggga
     4801 gggatgaagt attgaagtct acattagccc aaccatctcc atttgtatct gcgctatttg
     4861 gtaagttctc tccgtatacg gttttggcac catcctcagc attggctgtc gctgaaaaac
     4921 ctgtgaatgt taatgagcta gccaataata aagttggaag taatgatcgt tttttcataa
     4981 aattatctcc tttaatagaa tttgtatttt cattatcaca tagatacatt aaacataaaa
     5041 tgacttatcg gaagtgcata tgcttaacaa ttcacaaatg atataatata aaagtcgtcg
     5101 ttctacttct gtgctggttt agcatggaag gaggtgacac catgttcagc atcatatttg
     5161 tatctatagt agctcctatt atagtagggg ttataatcac gttgttttca cattggttga
     5221 ataatcgtga taaacaataa agcgacgaca cagtaataac accaaaaaga gcccgataac
     5281 tactggaata gttatcgggc tcggtgttta ccatgttcaa cacatatttg ttatctaaat
     5341 tataacatca tagtgtggag gaatgcaaaa ttgaataatt agtatgttaa gtctatacta
     5401 tactgattag taagtactaa ttatcatctt tgttattgtt acccctttca ttggcttttg
     5461 aatctaattc tttttgaaat tttttgtgtg attttgaaga agtgtttaaa aagaaatagg
     5521 tcatattatc catacgatgt ccttttataa attcattgat aaattgtttt tttgtcattt
     5581 ttttggttgt attacgttgt ttttgcaatc taatgatttc ttcttcaatg tatactctta
     5641 tcatcaaatc taaactagat gtctgattat aagtagtgaa taattttgga actaataaag
     5701 ttttattacc atcaaaaaga aatggagtga aatcataagt ttcccatata ccgttagtag
     5761 agaaataata atatgtcaca gtttctttag aaagaaacga tttcatttct ggaaatgact
     5821 gaatcatgga tttattaaaa tcaattataa cattcatgat tatattactt gttaaatctg
     5881 ataatttatc atcaccaaaa tttctgatta atggtgcttt ttgaggtatt gttaatccgt
     5941 catcatttaa gataatggaa agaatatctt cattcataaa tagattaatc aattcagatg
     6001 ctgtagtacc ttttcctgta ttacctttct gagaaaaacc taagcgtaaa tggtgtggtt
     6061 ctgaaaaatg agagaataat tttctaaatt cattacgctt ttgggtatca gaaaaatcac
     6121 taagatgtat atcgataatt ttttcaaaaa aaacatcaat ttgctcttgt gctctttgag
     6181 ccaaagtcgc aacttttcca acttctctat tagatattac tttaataagg tttgggtcta
     6241 tatattgaag agtgtcttct tcaatagaaa tatttaaata atcagttgat ttagcatcat
     6301 gattgaattt ggtacatact agtgtcactc tatatcatcc ttctgtaagt tttttgttta
     6361 cttccaatat agaatgatgt attattgtaa ttgtcgattt acaataagtt gttattctag
     6421 gtggaagttt attgcttttg atgtatagat agacttaacc ttttcattat agcatactat
     6481 agaagaaaat gcaagcaaaa gcatataaac aacaaatgtt gtttatatgc ttttgctttt
     6541 ttgttaattg gagaggacaa ggtgaatatt aacagtcaaa atccagttat gtcggttaat
     6601 gaatcgatga aaattttcaa agctcaagta atgcttagaa atttaaagct attaagcgag
     6661 tggattgtta aaggggactg aagttaaaga cactttttaa gaagattttt caattaaagg
     6721 gtatattaaa actttaattt agtactttat atttttcttg actctttaat aatcctatat
     6781 acagtagaac gactcactcc tgtttgttca gcaatttctt caccagacag cttttgttca
     6841 tcatataaga atttgatctc tcttttctta tgatctggta gtgaaggtct tccacctttt
     6901 ctccctcttg ctcttgcagc ttccaaacct tttttagttc gttcacttaa taaattagct
     6961 tcaagttcag cgaatgcact catcatggta aagaacatct tgcccatcgc atctttcgtt
     7021 gacacattca tatctataat atgtaaatca atgctatttt catccaacca ttgagatagc
     7081 tcaattaatt gttttgttgt acgtccaaga cgatctaatt tatagacaac tagaatatct
     7141 ccttcacgta aataatctaa acacttatca agttccgttc ttttagtttt acgaccactc
     7201 gctttctcgc taaagatacg ttcacaacca tattcctcaa gtgtatcaat ctgtccatca
     7261 agactttgat cttgtgttga cacacgtgca taaccaattt tagccatttg aacaccacct
     7321 tttttaatcg ttttatgggt acttactaat gaaatatagt tctcgatgta aataaacacg
     7381 tctgtcagca tctaaaaact catgattttc gatatttatg atacattgat tataacacaa
     7441 gtttatagac ttaaatatcc aagaaaaacc tcttaatatt aagttaagag gttttgtgtc
     7501 aaaaacgaac gtaatttaga aataatattt attggtattt caagaatata tatataatta
     7561 tttactaagt ccatttaaaa aaatttgaga gaacttgttc ataagtttaa ttctttcatt
     7621 aatattttgt tcatgtgtaa aggtaacaat tccattaaca gcgtttgctg ctattttact
     7681 aacagcatta acatcattaa tacaccattc gccatttaaa ttaccctcct taaaaattac
     7741 atgataagca tctatatatt tgttttctaa tttattcatt ttttcattaa tgctattagt
     7801 tttataatat tcagtgtaaa attcaattat tgcgttttga agtggataat agtattcggt
     7861 tgttaaagat agttcattat ataaataaaa tttttctcta ttagttttac atttgatttg
     7921 ttcctttttc cactgttctt gccatttaga ttcttctata tttaaaattt ctaaaaatag
     7981 attttctttt gttttaaagt gataataaag attcccttta ctactttctg ataatttaac
     8041 aatttctcca gtagtagtgg cattatatcc attttttata aataattcct ttgcgacacc
     8101 tagtatttta tctttcaagt tcatcacgcc tttaatgagt attaagtact atcaatgata
     8161 gcacaaaaaa ttcaaagaat aattgtataa cacaatacag ccatttaaat ttcgcaagat
     8221 tttttgttgt aatatgtaaa aaaaatagat tataatcctt atagaccgat cgcacggtct
     8281 ataaggattg gagggaactt aaatgatttc attttttaca aaaactactg atatgatgac
     8341 atcaaaaaaa agatggactg cactagtagt attagctgtt agtttgtttg ttgttacaat
     8401 ggatatgaca atattaatta tggctttacc ggaattagta agagagttag agccttctgg
     8461 tacccaacag ttatggatag ttgatatata ctctcttgtt ttagctggct ttataattcc
     8521 attgagtgcc tttgctgata aatggggaag aaaaaaagca ttattaactg gatttgcttt
     8581 atttggcctc gtttcattag ctatattttt cgcagaaagt gcagagttcg taatagctat
     8641 tcgattttta cttggtattg caggtgcttt aataatgcca actactcttt caatgataag
     8701 agtaattttt gaaaacccta aagaaagggc cactgcatta gctgtatggt caatcgcttc
     8761 atcgataggt gctgtttttg gaccaattat cggaggagct ttacttgagc aattttcatg
     8821 gcactcggca tttttaatta atgtaccgtt tgcgataata gcagttgtag caggtttatt
     8881 tttattacca gagtctaagt tatcaaaaga aaagtctcac tcgtgggata ttccttctac
     8941 aattttatca attgcaggca tgattggact ggtatggagt atcaaagaat tttcaaaaga
     9001 aggactagca gatattattc catgggttgt aatagtatta gcaattacca tgatagtgat
     9061 atttgttaaa cgtaatttat caagttctga tccaatgtta gacgtaagac tttttaaaaa
     9121 gagatcattt tcagctggta caattgctgc atttatgaca atttttgcaa tggcatctgt
     9181 tttgttatta gcttcacaat ggttacaggt tgtggaagaa ctttctcctt ttaaagctgg
     9241 cttataccta ttacctatgg caataggaga tatggtgttt gcaccaattg cacccggatt
     9301 agcggcgcga tttggaccga aaatagtgtt accttccgga attggaattg cagccattgg
     9361 catgtttatt atgtatttct ttggtcatcc attatcatat tctacaatgg ctttagcatt
     9421 aattttagtt ggagctggta tggcttcact agcagttgca tctgctctaa taatgttaga
     9481 aacacctaca tcaaaagcag gtaatgcagc tgctgttgaa gagtctatgt atgaccttgg
     9541 aaatgttttt ggtgtagcag tacttggtag cctatcttct atgctttatc gtgtattttt
     9601 agatatttca tctttttcat caaaaggtat agttggagat ttagctcatg tagctgaaga
     9661 atctgtagtg ggcgctgtcg aagtagctaa agctacgggg ataaaacagc ttgcaaacga
     9721 ggctgtaaca tcatttaatg atgcttttgt agcaactgct ttagtaggtg ggattatcat
     9781 gattatcatt tcaatagttg tctatttgtt aattcccaaa tcacttgata taactaaaca
     9841 aaaatagtac taaacatata tttttagata ttgtagatta gtttattttt aaaattagta
     9901 atagttaaaa agtagaacca ttaattttaa atggttctac ttttttatta ttaagtaaaa
     9961 ttataaaaga caatttctta aattaataat atgatatacc tatatctaat aatttattag
    10021 tattaataaa ccttgaaagg atgattaact gatgaataga gcagatattt ttaaaatatt
    10081 tgatgtagaa cttaatataa ctatattttt aggagcacta gtagcaactt tcatctattt
    10141 tataagttac atgaacaatg taaatgaatt aattacatta tgcttaacat tagcttttcc
    10201 tgcatattta ttaaaaatta gcagatcgtt cgaggttatt ttattagagg aaaaaataaa
    10261 aaatagtaaa atcaaaatct gtgacaaaga tagaattgaa cgagagatac tggtgaaaaa
    10321 gaacctaata gatgaactat tgtttcctaa tagagttatt aaaaaatata cactgacaat
    10381 tttaatttac agtataggta ttattattct tttactactt tgtaatatgt taagttatgg
    10441 aattggtgaa aatgtcaatt ttatctggag attactagct tgttatgtaa cgagtgtcac
    10501 agtgattata gttgccttga ttataatgat aatgatattt gatcttataa ttggattact
    10561 attaaatttt caaatatttt taaaaaataa aaagtatgat gattttttat aattattcga
    10621 tttatataat gaaaaattaa atgattaaac atagaaacct caaaacaaac catatttaat
    10681 agagtttatg gcgccttata caaataaagg ctatgctgtt aaagaattat gtagctatct
    10741 tcagattaat atggatgatg tttatactat tggcgataga aaattgattt acctatgttt
    10801 aataccctag ataattcttc tgataattta gtttttgtta gagtttaaca taactgatga
    10861 ttatcagaag tttgattcac attaaaaagg cacaacaagt tcatgaactt gtgtgccttt
    10921 ttaattgcaa ttagttacca aataaacgac tccaaaatct ctttttaggt ggttctacct
    10981 ccctattatc actggaatcg ttcttcgttt tttcaacaaa cttatcctct tttgttaatt
    11041 catctgtact gtcatgctta ttctcttcag ttatattttt atgatttact tcggttggtt
    11101 gacattcttt ttgttgttgg ttgatatttt gagattcttc agtaaaagtt gcttcttgtg
    11161 gattaatatt ctgtctatta ttcatcgttg tatcaaaaga atatttaagc tgtctttctt
    11221 cttctaattg gctttctagt ttttgaattt ttttattgtt ttctaaagat aatatttgtt
    11281 gattctccaa cagtttattt aatgtattaa cattgctatt ttgttgatct aattgtttag
    11341 ttagattctc aatatatttt tcatcatttt tagctctagt ttcaaagatt tctatttgct
    11401 tttctaattc gtttactttt gtttttaatg tctcagaact atttgaatta ttatgtatat
    11461 acttttcaac ttttgcagta ttttcattaa ataattcatt ttttactgat tctttattat
    11521 gagttgtttt ctctttctta gtgatttttt ctataatttt taaataatca ttatctttaa
    11581 tataattaat tccattttct ttttcaaaag atatatttaa tcttttagca ttattaacaa
    11641 tagtttgttt agttacacct aattcatcag caatcatttt aatagttttc ataacatccc
    11701 cccttaggta cctaatttag tacctaaatt ttaccaccta acaaaattta tttcatcatt
    11761 tagtactaaa tttactatct attaactacc taaatgccta attatctatc cttaaaatat
    11821 tttcgccact taattaggtg ctaaaatagc gaattatacg tttggtagtt ttaggtgtac
    11881 ttttaattac atttaaaact ctttatatac gccattaaaa gtgttaatat tactttataa
    11941 atattaaaag agtcgatgct attggcgtag catcgactct cggtaataaa acgattcgca
    12001 ctcgtttgtt tatatatttt tttgatactt gtattatata tatctaatca tctaagtgca
    12061 agcacaaaac atataactta cgtaaaaatt gttttattac ctcaatccca aaatggaaat
    12121 gaggttttta ttatgcccaa ttttgaaaaa tataatttat cacaagtaaa aactgaaaga
    12181 ttttatcaac tgcctaaata tttatttgaa gatgcatatt ttaagaaaat gtctgcagaa
    12241 gccaaaatta tgtatgcgtt attaaaagat cgttttgaat tatccctcca aaatgaatgg
    12301 gtagataaaa ataataatat ttactttatt ttcagtaata aacatttgtg tgaatactta
    12361 ggttatgcag aacaaaaaat tataaaatta aaaaaagagt taataaaatt taatttacta
    12421 actcaagaac gtgttggcct taataaacca aatagattat acctattaaa acctaattat
    12481 gacattgaag ccagtcatat caaggaactt ccaaattcac agttccagaa caatgaattt
    12541 ggaagttcta gaactgtgaa tttaagtggt caagaacttc caaattcaca gtctaatgat
    12601 actgattata atgacactga ttatattaag actaattata atgatatgta tgatttgaat
    12661 gataggaaat taacatattc tagtaatcat acaaatcatt caaatcacta ctattcaaaa
    12721 ttcaatgatg aagctttaaa atttcaatta cttgaagaac taccacaaag tattcaaagt
    12781 tatttaagta acttttctgt atctgaaatt aaacttatta aatctgtgtt attaaaagcc
    12841 aaaacatcct tcaacaattc tattgatgca tattatttat tagaagatat ggaatttgaa
    12901 attgttaatg ttcttaagcg ttttaaagct acattaattc aaaaaaatga aaccgttgaa
    12961 gcaatgcaag ggtacttaat gaaatctctt aaatctgaat ttgcagaagt acatacgctt
    13021 aataaacgac gtgatcattt accaatcact tctttattta atcaataata caacttaata
    13081 agccaacaat gatccaatta gagaatatct ttaattagat gttctcttaa ctgtaagcga
    13141 actttatttt caatgaaatg taattaatta agtaagtggg actaaaactt ttctttttta
    13201 gtcccatttt tataaacaat tattattcat tgttactact atcagcaatt ttttcttttt
    13261 agtgaaaccc aagtatttgt aagcatgata aattacttct ttttatattt attgggttta
    13321 tttgtattaa tgtagttgga atacatcttt aacaattctt ctgtttcatc aggacttaaa
    13381 tgctcacatt caaaatactt ttcaagcatt gcaccttttc gaatcagtct atgtgctctt
    13441 tcttttctat ctaaattttt taatctatgc tttcttaatt ctgctttttc aatttcagca
    13501 tttatttttt tgagatgttt aaaatcattc atcgtatcac ttactattct agttaatttt
    13561 gtcttctgtt
//
