LOCUS       NZ_CP073875            13570 bp    DNA     circular CON 16-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1285135 plasmid p1B1285135,
            complete sequence.
ACCESSION   NZ_CP073875
VERSION     NZ_CP073875.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699891
            Assembly: GCF_019329805.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 471x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019329805.1-RS_2024_04_16
            Annotation Date                   :: 04/16/2024 07:57:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,670
            CDSs (total)                      :: 2,589
            Genes (coding)                    :: 2,510
            CDSs (with protein)               :: 2,510
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 41 of 79
            Pseudo Genes (incomplete)         :: 44 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 24 of 79
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073875.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1285135"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p1B1285135"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(12064..13570,1..416))
                     /locus_tag="KFV37_RS00005"
                     /old_locus_tag="KFV37_00005"
     CDS             complement(join(12064..13570,1..416))
                     /locus_tag="KFV37_RS00005"
                     /old_locus_tag="KFV37_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            625..894
                     /locus_tag="KFV37_RS00010"
                     /old_locus_tag="KFV37_00010"
     CDS             625..894
                     /locus_tag="KFV37_RS00010"
                     /old_locus_tag="KFV37_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            911..1150
                     /locus_tag="KFV37_RS00015"
                     /old_locus_tag="KFV37_00015"
     CDS             911..1150
                     /locus_tag="KFV37_RS00015"
                     /old_locus_tag="KFV37_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(1375..2310)
                     /locus_tag="KFV37_RS00020"
                     /old_locus_tag="KFV37_00020"
     CDS             complement(1375..2310)
                     /locus_tag="KFV37_RS00020"
                     /old_locus_tag="KFV37_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            2751..3512
                     /locus_tag="KFV37_RS00025"
                     /old_locus_tag="KFV37_00025"
     CDS             2751..3512
                     /locus_tag="KFV37_RS00025"
                     /old_locus_tag="KFV37_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            complement(3831..4391)
                     /locus_tag="KFV37_RS00035"
                     /old_locus_tag="KFV37_00035"
     CDS             complement(3831..4391)
                     /locus_tag="KFV37_RS00035"
                     /old_locus_tag="KFV37_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(4596..6140)
                     /gene="qac"
                     /locus_tag="KFV37_RS00040"
                     /old_locus_tag="KFV37_00040"
     CDS             complement(4596..6140)
                     /gene="qac"
                     /locus_tag="KFV37_RS00040"
                     /old_locus_tag="KFV37_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            6319..6885
                     /gene="qacR"
                     /locus_tag="KFV37_RS00045"
                     /old_locus_tag="KFV37_00045"
     ARG             6320..6882
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            7136..7696
                     /locus_tag="KFV37_RS00050"
                     /old_locus_tag="KFV37_00050"
     CDS             7136..7696
                     /locus_tag="KFV37_RS00050"
                     /old_locus_tag="KFV37_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            8115..9026
                     /locus_tag="KFV37_RS00055"
                     /old_locus_tag="KFV37_00055"
     CDS             8115..9026
                     /locus_tag="KFV37_RS00055"
                     /old_locus_tag="KFV37_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(9203..9301)
                     /locus_tag="KFV37_RS00060"
                     /old_locus_tag="KFV37_00060"
     CDS             complement(9203..9301)
                     /locus_tag="KFV37_RS00060"
                     /old_locus_tag="KFV37_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            9465..10163
                     /locus_tag="KFV37_RS00065"
                     /old_locus_tag="KFV37_00065"
     CDS             9465..10163
                     /locus_tag="KFV37_RS00065"
                     /old_locus_tag="KFV37_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            10314..11018
                     /locus_tag="KFV37_RS00070"
                     /old_locus_tag="KFV37_00070"
     CDS             10314..11018
                     /locus_tag="KFV37_RS00070"
                     /old_locus_tag="KFV37_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(11067..11300)
                     /locus_tag="KFV37_RS00075"
                     /old_locus_tag="KFV37_00075"
     CDS             complement(11067..11300)
                     /locus_tag="KFV37_RS00075"
                     /old_locus_tag="KFV37_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(11377..11790)
                     /locus_tag="KFV37_RS13425"
                     /old_locus_tag="KFV37_00080"
     CDS             complement(11377..11790)
                     /locus_tag="KFV37_RS13425"
                     /old_locus_tag="KFV37_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     ARG             complement(4600..6140)
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          complement(1376..2310)
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4..416)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.5900000000000002e-85"
     Typing          complement(12134..13569)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 gcgtgtgggt tgtttatatc atctctatga attgaaatat cagcaaccat tcctttgtct
       61 acaaactcac tttttataaa atctttagtt aattcagttt gtacctctct atcaaaatca
      121 ttaggcaaac ttaataagac ttcttttgct aatctaggat tttttgtttt cgcattattc
      181 ttttcaactt tatcaacttc attccataaa cgctgtctat ctttagtcca ttcgggtgca
      241 tgttgaggag ttaaaatcat gctttcaggc ttaatagtat gatttctaaa cttaattttt
      301 tcatctgtac gttcgctata taatgcttca tcagaacgat aagacgctga cgcaacgata
      361 ttttttgttg ctcgattaat gttagtcact ctcaacataa agtaaccttt attcatgatt
      421 ttacctcctc tcttttctgt ttttttaccc ctgtgggaac tataatgatc aaccagtatg
      481 gttgcccatt tttatgccaa ttttcaattt gcatgtaaat gggcagtgca gtgtcttaag
      541 aactggttat attataccat ggatgtgcga cttacccaat gaattatggt atgctttata
      601 tagataaact aagggagttt tttaatgtct aaaattaaac aaacagatat tacaaaatta
      661 agtcaattag aaaaacaaat tgcacaactg aaagagcaaa agaaaaaaca agaagaacaa
      721 ctatctaata aaattggtgg ttatttttta aataaacttg atttaaatat gatcaactct
      781 agtgatgaat tgtataaaat tatggataaa gtcattggag aatttaataa agatatcgaa
      841 caaaatagca gcaacgagaa caaggaaaat ttaacagaag acaaaattaa ctagaatagt
      901 aagtgatacg atgaatgatt ttaaacatct caaaaaaata aatgctgaaa ttgaaaaagc
      961 agaattaaga aagcatagat taaaaaattt agatagaaaa gaaagagcac atagactgat
     1021 tcgaaaaggt gcaatgcttg aaaagtattt tgaatgtgag catttaagtc ctgatgaaac
     1081 agaagaattg ttaaagatgt attccaacta cattaataca aataaaccca ataaatataa
     1141 aaagaagtaa tttatcatgc ttacaaatac ttgggtttca ctaaaaagaa aaaattgctg
     1201 atagtagtaa caatgaataa taattgttta taaaaatggg actaaaaaag aaaagtttta
     1261 gtcccactta cttaattaat tacatttcat tgaaaataaa gttcgcttac agttaagaga
     1321 acatctaatt aaagatattc tctaattgga tcattgttgg cttattaagt tgtattattg
     1381 attaaataaa gaagtgattg gtaaatgatc acgtcgttta ttaagcgtat gtacttctgc
     1441 aaattcagat ttaagagatt tcattaagta cccttgcatt gcttcaacgg tttcattttt
     1501 ttgaattaat gtagctttaa aacgcttaag aacattaaca atttcaaatt ccatatcttc
     1561 taataaataa tatgcatcaa tagaattgtt gaaggatgtt ttggctttta ataacacaga
     1621 tttaataagt ttaatttcag atacagaaaa gttacttaaa taactttgaa tactttgtgg
     1681 tagttcttca agtaattgaa attttaaagc ttcatcattg aattttgaat agtagtgatt
     1741 tgaatgattt gtatgattac tagaatatgt taatttccta tcattcaaat catacatatc
     1801 attataatta gtcttaatat aatcagtgtc attataatca gtatcattag actgtgaatt
     1861 tggaagttct tgaccactta aattcacagt tctagaactt ccaaattcat tgttctggaa
     1921 ctgtgaattt ggaagttcct tgatatgact ggcttcaatg tcataattag gttttaatag
     1981 gtataatcta tttggtttat taaggccaac acgttcttga gttagtaaat taaattttat
     2041 taactctttt tttaatttta taattttttg ttctgcataa cctaagtatt cacacaaatg
     2101 tttattactg aaaataaagt aaatattatt atttttatct acccattcat tttggaggga
     2161 taattcaaaa cgatctttta ataacgcata cataattttg gcttctgcag acattttctt
     2221 aaaatatgca tcttcaaata aatatttagg cagttgataa aatctttcag tttttacttg
     2281 tgataaatta tatttttcaa aattgggcat aataaaaacc tcatttccat tttgggattg
     2341 aggtaataaa acaattttta cgtaagttat atgttttgtg cttgcactta gatgattaga
     2401 tatatataat acaagtatca aaaaaatata taaacaaacg agtgcgaatc gttttattac
     2461 cgagagtcga tgctacgcca atagcatcga ctcttttaat atttataaag taatattaac
     2521 acttttaatg gcgtatataa agagttttaa atgtaattaa aagtacacct aaaactacca
     2581 aacgtataat tcgctatttt agcacctaat taagtggcga aaatatttta aggatagata
     2641 attaggcatt taggtagtta atagatagta aatttagtac taaatgatga aataaatttt
     2701 gttaggtggt aaaatttagg tactaaatta ggtacctaag gggggatgtt atgaaaacta
     2761 ttaaaatgat tgctgatgaa ttaggtgtaa ctaaacaaac tattgttaat aatgctaaaa
     2821 gattaaatat atcttttgaa aaagaaaatg gaattaatta tattaaagat aatgattatt
     2881 taaaaattat agaaaaaatc actaagaaag agaaaacaac tcataataaa gaatcagtaa
     2941 aaaatgaatt atttaatgaa aatactgcaa aagttgaaaa gtatatacat aataattcaa
     3001 atagttctga gacattaaaa acaaaagtaa acgaattaga aaagcaaata gaaatctttg
     3061 aaactagagc taaaaatgat gaaaaatata ttgagaatct aactaaacaa ttagatcaac
     3121 aaaatagcaa tgttaataca ttaaataaac tgttggagaa tcaacaaata ttatctttag
     3181 aaaacaataa aaaaattcaa aaactagaaa gccaattaga agaagaaaga cagcttaaat
     3241 attcttttga tacaacgatg aataatagac agaatattaa tccacaagaa gcaactttta
     3301 ctgaagaatc tcaaaatatc aaccaacaac aaaaagaatg tcaaccaacc gaagtaaatc
     3361 ataaaaatat aactgaagag aataagcatg acagtacaga tgaattaaca aaagaggata
     3421 agtttgttga aaaaacgaag aacgattcca gtgataatag ggaggtagaa ccacctaaaa
     3481 agagattttg gagtcgttta tttggtaact aattgcaatt aaaaaggcac acaagttcat
     3541 gaacttgttg tgccttttta atgtgaatca aacttctgat aatcatcagt tatgttaaac
     3601 tctaacaaaa actaaattat cagaagaatt atctagggta ttaaacatag gtaaatcaat
     3661 tttctatcgc caatagtata aacatcatcc atattaatct gaagatagct acataattct
     3721 ttaacagcat agcctttatt tgtataaggc gccataaact ctattaaata tggtttgttt
     3781 tgaggtttct atgtttaatc atttaatttt tcattatata aatcgaataa ttataaaaaa
     3841 tcatcatact ttttattttt taaaaatatt tgaaaattta atagtaatcc aattataaga
     3901 tcaaatatca ttatcattat aatcaaggca actataatca ctgtgacact cgttacataa
     3961 caagctagta atctccagat aaaattgaca ttttcaccaa ttccataact taacatatta
     4021 caaagtagta aaagaataat aatacctata ctgtaaatta aaattgtcag tgtatatttt
     4081 ttaataactc tattaggaaa caatagttca tctattaggt tctttttcac cagtatctct
     4141 cgttcaattc tatctttgtc acagattttg attttactat tttttatttt ttcctctaat
     4201 aaaataacct cgaacgatct gctaattttt aataaatatg caggaaaagc taatgttaag
     4261 cataatgtaa ttaattcatt tacattgttc atgtaactta taaaatagat gaaagttgct
     4321 actagtgctc ctaaaaatat agttatatta agttctacat caaatatttt aaaaatatct
     4381 gctctattca tcagttaatc atcctttcaa ggtttattaa tactaataaa ttattagata
     4441 taggtatatc atattattaa tttaagaaat tgtcttttat aattttactt aataataaaa
     4501 aagtagaacc atttaaaatt aatggttcta ctttttaact attactaatt ttaaaaataa
     4561 actaatctac aatatctaaa aatatatgtt tagtactatt tttgtttagt tatatcaagt
     4621 gatttgggaa ttaacaaata gacaactatt gaaatgataa tcatgataat cccacctact
     4681 aaagcagttg ctacaaaagc atcattaaat gatgttacag cctcgtttgc aagctgtttt
     4741 atccccgtag ctttagctac ttcgacagcg cccactacag attcttcagc tacatgagct
     4801 aaatctccaa ctataccttt tgatgaaaaa gatgaaatat ctaaaaatac acgataaagc
     4861 atagaagata ggctaccaag tactgctaca ccaaaaacat ttccaaggtc atacatagac
     4921 tcttcaacag cagctgcatt acctgctttt gatgtaggtg tttctaacat tattagagca
     4981 gatgcaactg ctagtgaagc cataccagct ccaactaaaa ttaatgctaa agccattgta
     5041 gaatatgata atggatgacc aaagaaatac ataataaaca tgccaatggc tgcaattcca
     5101 attccggaag gtaacactat tttcggtcca aatcgcgccg ctaatccggg tgcaattggt
     5161 gcaaacacca tatctcctat tgccataggt aataggtata agccagcttt aaaaggagaa
     5221 agttcttcca caacctgtaa ccattgtgaa gctaataaca aaacagatgc cattgcaaaa
     5281 attgtcataa atgcagcaat tgtaccagct gaaaatgatc tctttttaaa aagtcttacg
     5341 tctaacattg gatcagaact tgataaatta cgtttaacaa atatcactat catggtaatt
     5401 gctaatacta ttacaaccca tggaataata tctgctagtc cttcttttga aaattctttg
     5461 atactccata ccagtccaat catgcctgca attgataaaa ttgtagaagg aatatcccac
     5521 gagtgagact tttcttttga taacttagac tctggtaata aaaataaacc tgctacaact
     5581 gctattatcg caaacggtac attaattaaa aatgccgagt gccatgaaaa ttgctcaagt
     5641 aaagctcctc cgataattgg tccaaaaaca gcacctatcg atgaagcgat tgaccataca
     5701 gctaatgcag tggccctttc tttagggttt tcaaaaatta ctcttatcat tgaaagagta
     5761 gttggcatta ttaaagcacc tgcaatacca agtaaaaatc gaatagctat tacgaactct
     5821 gcactttctg cgaaaaatat agctaatgaa acgaggccaa ataaagcaaa tccagttaat
     5881 aatgcttttt ttcttcccca tttatcagca aaggcactca atggaattat aaagccagct
     5941 aaaacaagag agtatatatc aactatccat aactgttggg taccagaagg ctctaactct
     6001 cttactaatt ccggtaaagc cataattaat attgtcatat ccattgtaac aacaaacaaa
     6061 ctaacagcta atactactag tgcagtccat cttttttttg atgtcatcat atcagtagtt
     6121 tttgtaaaaa atgaaatcat ttaagttccc tccaatcctt atagaccgtg cgatcggtct
     6181 ataaggatta taatctattt tttttacata ttacaacaaa aaatcttgcg aaatttaaat
     6241 ggctgtattg tgttatacaa ttattctttg aattttttgt gctatcattg atagtactta
     6301 atactcatta aaggcgtgat gaacttgaaa gataaaatac taggtgtcgc aaaggaatta
     6361 tttataaaaa atggatataa tgccactact actggagaaa ttgttaaatt atcagaaagt
     6421 agtaaaggga atctttatta tcactttaaa acaaaagaaa atctattttt agaaatttta
     6481 aatatagaag aatctaaatg gcaagaacag tggaaaaagg aacaaatcaa atgtaaaact
     6541 aatagagaaa aattttattt atataatgaa ctatctttaa caaccgaata ctattatcca
     6601 cttcaaaacg caataattga attttacact gaatattata aaactaatag cattaatgaa
     6661 aaaatgaata aattagaaaa caaatatata gatgcttatc atgtaatttt taaggagggt
     6721 aatttaaatg gcgaatggtg tattaatgat gttaatgctg ttagtaaaat agcagcaaac
     6781 gctgttaatg gaattgttac ctttacacat gaacaaaata ttaatgaaag aattaaactt
     6841 atgaacaagt tctctcaaat ttttttaaat ggacttagta aataattata tatatattct
     6901 tgaaatacca ataaatatta tttctaaatt acgttcgttt ttgacacaaa acctcttaac
     6961 ttaatattaa gaggtttttc ttggatattt aagtctataa acttgtgtta taatcaatgt
     7021 atcataaata tcgaaaatca tgagttttta gatgctgaca gacgtgttta tttacatcga
     7081 gaactatatt tcattagtaa gtacccataa aacgattaaa aaaggtggtg ttcaaatggc
     7141 taaaattggt tatgcacgtg tgtcaacaca agatcaaagt cttgatggac agattgatac
     7201 acttgaggaa tatggttgtg aacgtatctt tagcgagaaa gcgagtggtc gtaaaactaa
     7261 aagaacggaa cttgataagt gtttagatta tttacgtgaa ggagatattc tagttgtcta
     7321 taaattagat cgtcttggac gtacaacaaa acaattaatt gagctatctc aatggttgga
     7381 tgaaaatagc attgatttac atattataga tatgaatgtg tcaacgaaag atgcgatggg
     7441 caagatgttc tttaccatga tgagtgcatt cgctgaactt gaagctaatt tattaagtga
     7501 acgaactaaa aaaggtttgg aagctgcaag agcaagaggg agaaaaggtg gaagaccttc
     7561 actaccagat cataagaaaa gagagatcaa attcttatat gatgaacaaa agctgtctgg
     7621 tgaagaaatt gctgaacaaa caggagtgag tcgttctact gtatatagga ttattaaaga
     7681 gtcaagaaaa atataaagta ctaaattaaa gttttaatat accctttaat tgaaaaatct
     7741 tcttaaaaag tgtctttaac ttcagtcccc tttaacaatc cactcgctta atagctttaa
     7801 atttctaagc attacttgag ctttgaaaat tttcatcgat tcattaaccg acataactgg
     7861 attttgactg ttaatattca ccttgtcctc tccaattaac aaaaaagcaa aagcatataa
     7921 acaacatttg ttgtttatat gcttttgctt gcattttctt ctatagtatg ctataatgaa
     7981 aaggttaagt ctatctatac atcaaaagca ataaacttcc acctagaata acaacttatt
     8041 gtaaatcgac aattacaata atacatcatt ctatattgga agtaaacaaa aaacttacag
     8101 aaggatgata tagagtgaca ctagtatgta ccaaattcaa tcatgatgct aaatcaactg
     8161 attatttaaa tatttctatt gaagaagaca ctcttcaata tatagaccca aaccttatta
     8221 aagtaatatc taatagagaa gttggaaaag ttgcgacttt ggctcaaaga gcacaagagc
     8281 aaattgatgt tttttttgaa aaaattatcg atatacatct tagtgatttt tctgataccc
     8341 aaaagcgtaa tgaatttaga aaattattct ctcatttttc agaaccacac catttacgct
     8401 taggtttttc tcagaaaggt aatacaggaa aaggtactac agcatctgaa ttgattaatc
     8461 tatttatgaa tgaagatatt ctttccatta tcttaaatga tgacggatta acaatacctc
     8521 aaaaagcacc attaatcaga aattttggtg atgataaatt atcagattta acaagtaata
     8581 taatcatgaa tgttataatt gattttaata aatccatgat tcagtcattt ccagaaatga
     8641 aatcgtttct ttctaaagaa actgtgacat attattattt ctctactaac ggtatatggg
     8701 aaacttatga tttcactcca tttctttttg atggtaataa aactttatta gttccaaaat
     8761 tattcactac ttataatcag acatctagtt tagatttgat gataagagta tacattgaag
     8821 aagaaatcat tagattgcaa aaacaacgta atacaaccaa aaaaatgaca aaaaaacaat
     8881 ttatcaatga atttataaaa ggacatcgta tggataatat gacctatttc tttttaaaca
     8941 cttcttcaaa atcacacaaa aaatttcaaa aagaattaga ttcaaaagcc aatgaaaggg
     9001 gtaacaataa caaagatgat aattagtact tactaatcag tatagtatag acttaacata
     9061 ctaattattc aattttgcat tcctccacac tatgatgtta taatttagat aacaaatatg
     9121 tgttgaacat ggtaaacacc gagcccgata actattccag tagttatcgg gctctttttg
     9181 gtgttattac tgtgtcgtcg ctttattgtt tatcacgatt attcaaccaa tgtgaaaaca
     9241 acgtgattat aacccctact ataataggag ctactataga tacaaatatg atgctgaaca
     9301 tggtgtcacc tccttccatg ctaaaccagc acagaagtag aacgacgact tttatattat
     9361 atcatttgtg aattgttaag catatgcact tccgataagt cattttatgt ttaatgtatc
     9421 tatgtgataa tgaaaataca aattctatta aaggagataa ttttatgaaa aaacgatcat
     9481 tacttccaac tttattattg gctagctcat taacattcac aggtttttca gcgacagcca
     9541 atgctgagga tggtgccaaa accgtatacg gagagaactt accaaatagc gcagatacaa
     9601 atggagatgg ttgggctaat gtagacttca atacttcatc cctcccacaa tctgcacaaa
     9661 atcaaataag tgaactttct aaacagaaag attcagggga attaactcaa atcgagtata
     9721 atgagaaagt ttcaagtgta tttaatgaat atcaatcaca aagtactgat atacaaaata
     9781 atgagagaac tcaacaaggt aaaagacctt tcggaggggt gatacctaat tgtatgactc
     9841 aagaagaata ccaagaatta gaaagtcatg ttcctaaccc taatagtgta tccacagaaa
     9901 aatataatca aattgtaaaa aatgaaagtc aaaagatagc taatgaagaa aataaaacta
     9961 ttaatacacc tcaaggtgtg ttgacacctg taactgaaaa taaaaacaag agttctgaag
    10021 gaatgaaaaa tcaagattca aactctcaag tattaccaga aacaggagaa agcaaccaaa
    10081 gtgatatatt gtcaataatt ggcgcttctg gactactaat attaggtata agtcttttaa
    10141 tattcagaaa aaaggcaata taaaaattga taaaataacc accgtgttta tgtaaaacat
    10201 gtggttattt tattatatat ttaatacata tttttcaact catgtaattt acgttttctt
    10261 gctgaaaaaa tattaacata atctaatttg aaatactgaa aaggagttgt aatatgaaaa
    10321 aaatcatttt acctatatta attgttagtt tatcggtaat tttagtgagt tgtaataata
    10381 acgattcaga acatcacaat gataataatt ctagtgatac taacacctca gaaaacaaag
    10441 ataatgaagc atcaaatgaa attgacgaaa aaactaacac atctgatagt gcggataatt
    10501 cagaaagtag taataaacaa aataaagaaa aaaatgataa taaaaaacaa gaaaaaaata
    10561 atgatagtaa taagaagcaa caatctactg ataaaaataa aagttactta aataatttta
    10621 actcgaaaga aattgaatat gcacgagttt ggtatcagtt aatatctaca agaaatgatt
    10681 taaaaggtat taaaaatgtt tatgttacag aaataccaaa aggctcaaaa gtaaatccac
    10741 aagccaaaaa tagtgcaatt tataaagaaa aagttgtgaa attagaagct cctatgagag
    10801 ctggtgggtc tgtaacatat agcagtaatg gtgatggaac aattaatgta tataataata
    10861 tcccttataa atgggaaaat tcacaaaata gtgattatag tcaaatgaat aagataacaa
    10921 aaaaagcaat agaggagaat attgaaacag tctatattaa accctacgac aataaaacag
    10981 tagctaagtt agcaagtaaa attagatata ataagtagtt agtagattat taaaaaggcg
    11041 cctaaaatta ggcgtctttt ttatcattag ttcaaaagat aagcataacg ttccatgtaa
    11101 cagcatataa agtaatgaat caattcatca ctatctaatg ccaccattgt tagttcagcc
    11161 ttgcatggtt taacgtatct cacataatgg tacaaatctt caaatagtgc ctgtttttcg
    11221 ggcattttaa acaccttgtg tacattaaaa cctatttctt ctaatcgctt tgtaatcacg
    11281 ccgaaaacga atgtgttcat gtgaaatcac tccatcattt ttaatattca catgtacact
    11341 aaaaaaaatc cgatacatcg gctttttatt tttggtttaa tttatttcta tattgttttc
    11401 actatcgctc ggacgcataa gtgttaaaac accttctacc atacctatca cacccggtat
    11461 tcccgtccaa caaaatagta aataaagaag agcattttta tttttcttaa cataaaactt
    11521 atgagccccg aaacctccta gaaaaaatgc taatgcagag taaacatatt tattaacttg
    11581 catgatttcc actcctttaa ttaattgatt tagagaaaca ttaaataatt tagtgatttc
    11641 taaaattgtt tttaagtcgg gattacttac ttctctttcc caattagaaa tagaatgtct
    11701 cgatacccct aacttttcag ctaatttttc ttgagacatg tttttacttt ttcttaactt
    11761 ttgtatttga tgtccaattt ttgtttccaa attatttttc tccttaactt taagatatat
    11821 ttaaactcaa ttttttctat cgatatttat gaacattagt attttttatg ctgaaatatg
    11881 tgaacaatcg cataatatca aggtttttct ataattatac acgttttaaa atacattgtg
    11941 ataatacata gaatgtacct tatctaagta tataaatgtt ttctttaaac caaagctaat
    12001 ttaccacatt gggttaagtt tcttattatt aaagaatgaa ctttgtatga tttcaactgc
    12061 ttactatctt ctcatttggt tatttctctc ttgtttcttc ttctttttct gatgattaat
    12121 attgttttgc ttttcagttt cgttcgcata gtacataaga aagtcactag catttatcgt
    12181 tggtaaatta atgtgattag tttgttcatt ttcatgttca atacgattgt catgatcatt
    12241 tctatctact tgattaatat gtttttcgat aacttcttct tgatcgacat gattatattt
    12301 ctcaagttta atgagtaatt gaactttttc attaggtgtt tggtctttga ctttttcaaa
    12361 gtttttatcg cctatatcta ttcttaaatc atcatcaata tgttctttat aactatcaat
    12421 ttctttttct ttttccttaa taaattgcaa ctcaacgtta tattcaaagc tatctttttc
    12481 taaattttta agatgttttt gactaaagaa aatatcttta ctcgcttttt tataagtatc
    12541 ttctagcaat acatcattag gaatattgtt attttgtttg aacaggtatt gaacatgagt
    12601 tgcttttata atattacctt tttgtgcctc atcaacaata aatgcttttt catcatggct
    12661 aaaatgatga tgagatttag ggtacatttg ctgaacaatg agatgatttt tttcactttc
    12721 agttagataa acatctttag ctgattgata aatctcttta cgtttttcct ctctactttc
    12781 taatttattc ttctcattga gtaacgcttt atagttagtt tcaacagtat taacaaactc
    12841 ttgttgacta tatcctttac tttcaatttc ttttctaaac tgataatttt catcataatt
    12901 tttagcatag aattttaatt gacgtctctt aatttcattt ttaagtaaat cattttgaat
    12961 tttacgacca aaggtagacg cactgtcaaa cgtattttta attgtttctt tcgcctctga
    13021 atagccaaca tcacgttgat atctatcaaa taaaatagaa taactttgtt catttttact
    13081 tacttgttcg ttagcatttt gaataaataa tctaatttca ttttgtgctt gttctttctc
    13141 tttatcacta aataattgtg atacttcgcc acgattatat cttctaattt gttcattttt
    13201 ctgtccgtaa taagtaacgg gttggtactc gataccctct ttttcacagc gttttctttc
    13261 attttgttct aatctatatt cttctcttgt aagtggaatt tcggcaattt tttctaaacc
    13321 ttgcgcttca aaagaacgac tatcatatcg tcttaaactt tgttcacgtt ctgagtattg
    13381 atttaatttt aattcccaat gcttacgaac ttctttgaaa tcaaagtcgg caaatcgttc
    13441 ttgttttcta actttattac catttttatt taaaatcgca ttgccatttt catcatattg
    13501 cgttctcgtt tttgtctttt tcccccaagt accgtctgca ttaaacggtc tttgagtaag
    13561 tagaacatgg
//
