LOCUS       NZ_CP073871            13570 bp    DNA     circular CON 17-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1282731 plasmid p1B1282731,
            complete sequence.
ACCESSION   NZ_CP073871
VERSION     NZ_CP073871.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699890
            Assembly: GCF_019329785.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 407x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019329785.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 07:05:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,685
            CDSs (total)                      :: 2,604
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 41 of 78
            Pseudo Genes (incomplete)         :: 43 of 78
            Pseudo Genes (internal stop)      :: 27 of 78
            Pseudo Genes (multiple problems)  :: 24 of 78
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073871.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1282731"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p1B1282731"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(13402..13570,1..71))
                     /locus_tag="KFV34_RS00005"
                     /old_locus_tag="KFV34_00005"
     CDS             complement(join(13402..13570,1..71))
                     /locus_tag="KFV34_RS00005"
                     /old_locus_tag="KFV34_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(88..357)
                     /locus_tag="KFV34_RS00010"
                     /old_locus_tag="KFV34_00010"
     CDS             complement(88..357)
                     /locus_tag="KFV34_RS00010"
                     /old_locus_tag="KFV34_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            566..2488
                     /locus_tag="KFV34_RS00015"
                     /old_locus_tag="KFV34_00015"
     CDS             566..2488
                     /locus_tag="KFV34_RS00015"
                     /old_locus_tag="KFV34_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            2762..3175
                     /locus_tag="KFV34_RS13500"
                     /old_locus_tag="KFV34_00020"
     CDS             2762..3175
                     /locus_tag="KFV34_RS13500"
                     /old_locus_tag="KFV34_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            3252..3485
                     /locus_tag="KFV34_RS00025"
                     /old_locus_tag="KFV34_00025"
     CDS             3252..3485
                     /locus_tag="KFV34_RS00025"
                     /old_locus_tag="KFV34_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(3534..4238)
                     /locus_tag="KFV34_RS00030"
                     /old_locus_tag="KFV34_00030"
     CDS             complement(3534..4238)
                     /locus_tag="KFV34_RS00030"
                     /old_locus_tag="KFV34_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(4389..5087)
                     /locus_tag="KFV34_RS00035"
                     /old_locus_tag="KFV34_00035"
     CDS             complement(4389..5087)
                     /locus_tag="KFV34_RS00035"
                     /old_locus_tag="KFV34_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            5251..5349
                     /locus_tag="KFV34_RS00040"
                     /old_locus_tag="KFV34_00040"
     CDS             5251..5349
                     /locus_tag="KFV34_RS00040"
                     /old_locus_tag="KFV34_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(5526..6437)
                     /locus_tag="KFV34_RS00045"
                     /old_locus_tag="KFV34_00045"
     CDS             complement(5526..6437)
                     /locus_tag="KFV34_RS00045"
                     /old_locus_tag="KFV34_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(6856..7416)
                     /locus_tag="KFV34_RS00050"
                     /old_locus_tag="KFV34_00050"
     CDS             complement(6856..7416)
                     /locus_tag="KFV34_RS00050"
                     /old_locus_tag="KFV34_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            complement(7667..8233)
                     /gene="qacR"
                     /locus_tag="KFV34_RS00055"
                     /old_locus_tag="KFV34_00055"
     ARG             complement(7671..8233)
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            8412..9956
                     /gene="qac"
                     /locus_tag="KFV34_RS00060"
                     /old_locus_tag="KFV34_00060"
     CDS             8412..9956
                     /gene="qac"
                     /locus_tag="KFV34_RS00060"
                     /old_locus_tag="KFV34_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            10161..10721
                     /locus_tag="KFV34_RS00065"
                     /old_locus_tag="KFV34_00065"
     CDS             10161..10721
                     /locus_tag="KFV34_RS00065"
                     /old_locus_tag="KFV34_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(11040..11801)
                     /locus_tag="KFV34_RS00075"
                     /old_locus_tag="KFV34_00075"
     CDS             complement(11040..11801)
                     /locus_tag="KFV34_RS00075"
                     /old_locus_tag="KFV34_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            12242..13177
                     /locus_tag="KFV34_RS00080"
                     /old_locus_tag="KFV34_00080"
     CDS             12242..13177
                     /locus_tag="KFV34_RS00080"
                     /old_locus_tag="KFV34_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     ARG             8413..9953
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          12243..13177
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          567..2419
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 aatctatgct ttcttaattc tgctttttca atttcagcat ttattttttt gagatgttta
       61 aaatcattca tcgtatcact tactattcta gttaattttg tcttctgtta aattttcctt
      121 gttctcgttg ctgctatttt gttcgatatc tttattaaat tctccaatga ctttatccat
      181 aattttatac aattcatcac tagagttgat catatttaaa tcaagtttat ttaaaaaata
      241 accaccaatt ttattagata gttgttcttc ttgttttttc ttttgctctt tcagttgtgc
      301 aatttgtttt tctaattgac ttaattttgt aatatctgtt tgtttaattt tagacattaa
      361 aaaactccct tagtttatct atataaagca taccataatt cattgggtaa gtcgcacatc
      421 catggtataa tataaccagt tcttaagaca ctgcactgcc catttacatg caaattgaaa
      481 attggcataa aaatgggcaa ccatactggt tgatcattat agttcccaca ggggtaaaaa
      541 aacagaaaag agaggaggta aaatcatgaa taaaggttac tttatgttga gagtgactaa
      601 cattaatcga gcaacaaaaa atatcgttgc gtcagcgtct tatcgttctg atgaagcatt
      661 atatagcgaa cgtacagatg aaaaaattaa gtttagaaat catactatta agcctgaaag
      721 catgatttta actcctcaac atgcacccga atggactaaa gatagacagc gtttatggaa
      781 tgaagttgat aaagttgaaa agaataatgc gaaaacaaaa aatcctagat tagcaaaaga
      841 agtcttatta agtttgccta atgattttga tagagaggta caaactgaat taactaaaga
      901 ttttataaaa agtgagtttg tagacaaagg aatggttgct gatatttcaa ttcatagaga
      961 tgatataaac aacccacacg cccatgttct acttactcaa agaccgttta atgcagacgg
     1021 tacttggggg aaaaagacaa aaacgagaac gcaatatgat gaaaatggca atgcgatttt
     1081 aaataaaaat ggtaataaag ttagaaaaca agaacgattt gccgactttg atttcaaaga
     1141 agttcgtaag cattgggaat taaaattaaa tcaatactca gaacgtgaac aaagtttaag
     1201 acgatatgat agtcgttctt ttgaagcgca aggtttagaa aaaattgccg aaattccact
     1261 tacaagagaa gaatatagat tagaacaaaa tgaaagaaaa cgctgtgaaa aagagggtat
     1321 cgagtaccaa cccgttactt attacggaca gaaaaatgaa caaattagaa gatataatcg
     1381 tggcgaagta tcacaattat ttagtgataa agagaaagaa caagcacaaa atgaaattag
     1441 attatttatt caaaatgcta acgaacaagt aagtaaaaat gaacaaagtt attctatttt
     1501 atttgataga tatcaacgtg atgttggcta ttcagaggcg aaagaaacaa ttaaaaatac
     1561 gtttgacagt gcgtctacct ttggtcgtaa aattcaaaat gatttactta aaaatgaaat
     1621 taagagacgt caattaaaat tctatgctaa aaattatgat gaaaattatc agtttagaaa
     1681 agaaattgaa agtaaaggat atagtcaaca agagtttgtt aatactgttg aaactaacta
     1741 taaagcgtta ctcaatgaga agaataaatt agaaagtaga gaggaaaaac gtaaagagat
     1801 ttatcaatca gctaaagatg tttatctaac tgaaagtgaa aaaaatcatc tcattgttca
     1861 gcaaatgtac cctaaatctc atcatcattt tagccatgat gaaaaagcat ttattgttga
     1921 tgaggcacaa aaaggtaata ttataaaagc aactcatgtt caatacctgt tcaaacaaaa
     1981 taacaatatt cctaatgatg tattgctaga agatacttat aaaaaagcga gtaaagatat
     2041 tttctttagt caaaaacatc ttaaaaattt agaaaaagat agctttgaat ataacgttga
     2101 gttgcaattt attaaggaaa aagaaaaaga aattgatagt tataaagaac atattgatga
     2161 tgatttaaga atagatatag gcgataaaaa ctttgaaaaa gtcaaagacc aaacacctaa
     2221 tgaaaaagtt caattactca ttaaacttga gaaatataat catgtcgatc aagaagaagt
     2281 tatcgaaaaa catattaatc aagtagatag aaatgatcat gacaatcgta ttgaacatga
     2341 aaatgaacaa actaatcaca ttaatttacc aacgataaat gctagtgact ttcttatgta
     2401 ctatgcgaac gaaactgaaa agcaaaacaa tattaatcat cagaaaaaga agaagaaaca
     2461 agagagaaat aaccaaatga gaagatagta agcagttgaa atcatacaaa gttcattctt
     2521 taataataag aaacttaacc caatgtggta aattagcttt ggtttaaaga aaacatttat
     2581 atacttagat aaggtacatt ctatgtatta tcacaatgta ttttaaaacg tgtataatta
     2641 tagaaaaacc ttgatattat gcgattgttc acatatttca gcataaaaaa tactaatgtt
     2701 cataaatatc gatagaaaaa attgagttta aatatatctt aaagttaagg agaaaaataa
     2761 tttggaaaca aaaattggac atcaaataca aaagttaaga aaaagtaaaa acatgtctca
     2821 agaaaaatta gctgaaaagt taggggtatc gagacattct atttctaatt gggaaagaga
     2881 agtaagtaat cccgacttaa aaacaatttt agaaatcact aaattattta atgtttctct
     2941 aaatcaatta attaaaggag tggaaatcat gcaagttaat aaatatgttt actctgcatt
     3001 agcatttttt ctaggaggtt tcggggctca taagttttat gttaagaaaa ataaaaatgc
     3061 tcttctttat ttactatttt gttggacggg aataccgggt gtgataggta tggtagaagg
     3121 tgttttaaca cttatgcgtc cgagcgatag tgaaaacaat atagaaataa attaaaccaa
     3181 aaataaaaag ccgatgtatc ggattttttt tagtgtacat gtgaatatta aaaatgatgg
     3241 agtgatttca catgaacaca ttcgttttcg gcgtgattac aaagcgatta gaagaaatag
     3301 gttttaatgt acacaaggtg tttaaaatgc ccgaaaaaca ggcactattt gaagatttgt
     3361 accattatgt gagatacgtt aaaccatgca aggctgaact aacaatggtg gcattagata
     3421 gtgatgaatt gattcattac tttatatgct gttacatgga acgttatgct tatcttttga
     3481 actaatgata aaaaagacgc ctaattttag gcgccttttt aataatctac taactactta
     3541 ttatatctaa ttttacttgc taacttagct actgttttat tgtcgtaggg tttaatatag
     3601 actgtttcaa tattctcctc tattgctttt tttgttatct tattcatttg actataatca
     3661 ctattttgtg aattttccca tttataaggg atattattat atacattaat tgttccatca
     3721 ccattactgc tatatgttac agacccacca gctctcatag gagcttctaa tttcacaact
     3781 ttttctttat aaattgcact atttttggct tgtggattta cttttgagcc ttttggtatt
     3841 tctgtaacat aaacattttt aatacctttt aaatcatttc ttgtagatat taactgatac
     3901 caaactcgtg catattcaat ttctttcgag ttaaaattat ttaagtaact tttattttta
     3961 tcagtagatt gttgcttctt attactatca ttattttttt cttgtttttt attatcattt
     4021 ttttctttat tttgtttatt actactttct gaattatccg cactatcaga tgtgttagtt
     4081 ttttcgtcaa tttcatttga tgcttcatta tctttgtttt ctgaggtgtt agtatcacta
     4141 gaattattat cattgtgatg ttctgaatcg ttattattac aactcactaa aattaccgat
     4201 aaactaacaa ttaatatagg taaaatgatt tttttcatat tacaactcct tttcagtatt
     4261 tcaaattaga ttatgttaat attttttcag caagaaaacg taaattacat gagttgaaaa
     4321 atatgtatta aatatataat aaaataacca catgttttac ataaacacgg tggttatttt
     4381 atcaattttt atattgcctt ttttctgaat attaaaagac ttatacctaa tattagtagt
     4441 ccagaagcgc caattattga caatatatca ctttggttgc tttctcctgt ttctggtaat
     4501 acttgagagt ttgaatcttg atttttcatt ccttcagaac tcttgttttt attttcagtt
     4561 acaggtgtca acacaccttg aggtgtatta atagttttat tttcttcatt agctatcttt
     4621 tgactttcat tttttacaat ttgattatat ttttctgtgg atacactatt agggttagga
     4681 acatgacttt ctaattcttg gtattcttct tgagtcatac aattaggtat cacccctccg
     4741 aaaggtcttt taccttgttg agttctctca ttattttgta tatcagtact ttgtgattga
     4801 tattcattaa atacacttga aactttctca ttatactcga tttgagttaa ttcccctgaa
     4861 tctttctgtt tagaaagttc acttatttga ttttgtgcag attgtgggag ggatgaagta
     4921 ttgaagtcta cattagccca accatctcca tttgtatctg cgctatttgg taagttctct
     4981 ccgtatacgg ttttggcacc atcctcagca ttggctgtcg ctgaaaaacc tgtgaatgtt
     5041 aatgagctag ccaataataa agttggaagt aatgatcgtt ttttcataaa attatctcct
     5101 ttaatagaat ttgtattttc attatcacat agatacatta aacataaaat gacttatcgg
     5161 aagtgcatat gcttaacaat tcacaaatga tataatataa aagtcgtcgt tctacttctg
     5221 tgctggttta gcatggaagg aggtgacacc atgttcagca tcatatttgt atctatagta
     5281 gctcctatta tagtaggggt tataatcacg ttgttttcac attggttgaa taatcgtgat
     5341 aaacaataaa gcgacgacac agtaataaca ccaaaaagag cccgataact actggaatag
     5401 ttatcgggct cggtgtttac catgttcaac acatatttgt tatctaaatt ataacatcat
     5461 agtgtggagg aatgcaaaat tgaataatta gtatgttaag tctatactat actgattagt
     5521 aagtactaat tatcatcttt gttattgtta cccctttcat tggcttttga atctaattct
     5581 ttttgaaatt ttttgtgtga ttttgaagaa gtgtttaaaa agaaataggt catattatcc
     5641 atacgatgtc cttttataaa ttcattgata aattgttttt ttgtcatttt tttggttgta
     5701 ttacgttgtt tttgcaatct aatgatttct tcttcaatgt atactcttat catcaaatct
     5761 aaactagatg tctgattata agtagtgaat aattttggaa ctaataaagt tttattacca
     5821 tcaaaaagaa atggagtgaa atcataagtt tcccatatac cgttagtaga gaaataataa
     5881 tatgtcacag tttctttaga aagaaacgat ttcatttctg gaaatgactg aatcatggat
     5941 ttattaaaat caattataac attcatgatt atattacttg ttaaatctga taatttatca
     6001 tcaccaaaat ttctgattaa tggtgctttt tgaggtattg ttaatccgtc atcatttaag
     6061 ataatggaaa gaatatcttc attcataaat agattaatca attcagatgc tgtagtacct
     6121 tttcctgtat tacctttctg agaaaaacct aagcgtaaat ggtgtggttc tgaaaaatga
     6181 gagaataatt ttctaaattc attacgcttt tgggtatcag aaaaatcact aagatgtata
     6241 tcgataattt tttcaaaaaa aacatcaatt tgctcttgtg ctctttgagc caaagtcgca
     6301 acttttccaa cttctctatt agatattact ttaataaggt ttgggtctat atattgaaga
     6361 gtgtcttctt caatagaaat atttaaataa tcagttgatt tagcatcatg attgaatttg
     6421 gtacatacta gtgtcactct atatcatcct tctgtaagtt ttttgtttac ttccaatata
     6481 gaatgatgta ttattgtaat tgtcgattta caataagttg ttattctagg tggaagttta
     6541 ttgcttttga tgtatagata gacttaacct tttcattata gcatactata gaagaaaatg
     6601 caagcaaaag catataaaca acaaatgttg tttatatgct tttgcttttt tgttaattgg
     6661 agaggacaag gtgaatatta acagtcaaaa tccagttatg tcggttaatg aatcgatgaa
     6721 aattttcaaa gctcaagtaa tgcttagaaa tttaaagcta ttaagcgagt ggattgttaa
     6781 aggggactga agttaaagac actttttaag aagatttttc aattaaaggg tatattaaaa
     6841 ctttaattta gtactttata tttttcttga ctctttaata atcctatata cagtagaacg
     6901 actcactcct gtttgttcag caatttcttc accagacagc ttttgttcat catataagaa
     6961 tttgatctct cttttcttat gatctggtag tgaaggtctt ccaccttttc tccctcttgc
     7021 tcttgcagct tccaaacctt ttttagttcg ttcacttaat aaattagctt caagttcagc
     7081 gaatgcactc atcatggtaa agaacatctt gcccatcgca tctttcgttg acacattcat
     7141 atctataata tgtaaatcaa tgctattttc atccaaccat tgagatagct caattaattg
     7201 ttttgttgta cgtccaagac gatctaattt atagacaact agaatatctc cttcacgtaa
     7261 ataatctaaa cacttatcaa gttccgttct tttagtttta cgaccactcg ctttctcgct
     7321 aaagatacgt tcacaaccat attcctcaag tgtatcaatc tgtccatcaa gactttgatc
     7381 ttgtgttgac acacgtgcat aaccaatttt agccatttga acaccacctt ttttaatcgt
     7441 tttatgggta cttactaatg aaatatagtt ctcgatgtaa ataaacacgt ctgtcagcat
     7501 ctaaaaactc atgattttcg atatttatga tacattgatt ataacacaag tttatagact
     7561 taaatatcca agaaaaacct cttaatatta agttaagagg ttttgtgtca aaaacgaacg
     7621 taatttagaa ataatattta ttggtatttc aagaatatat atataattat ttactaagtc
     7681 catttaaaaa aatttgagag aacttgttca taagtttaat tctttcatta atattttgtt
     7741 catgtgtaaa ggtaacaatt ccattaacag cgtttgctgc tattttacta acagcattaa
     7801 catcattaat acaccattcg ccatttaaat taccctcctt aaaaattaca tgataagcat
     7861 ctatatattt gttttctaat ttattcattt tttcattaat gctattagtt ttataatatt
     7921 cagtgtaaaa ttcaattatt gcgttttgaa gtggataata gtattcggtt gttaaagata
     7981 gttcattata taaataaaat ttttctctat tagttttaca tttgatttgt tcctttttcc
     8041 actgttcttg ccatttagat tcttctatat ttaaaatttc taaaaataga ttttcttttg
     8101 ttttaaagtg ataataaaga ttccctttac tactttctga taatttaaca atttctccag
     8161 tagtagtggc attatatcca ttttttataa ataattcctt tgcgacacct agtattttat
     8221 ctttcaagtt catcacgcct ttaatgagta ttaagtacta tcaatgatag cacaaaaaat
     8281 tcaaagaata attgtataac acaatacagc catttaaatt tcgcaagatt ttttgttgta
     8341 atatgtaaaa aaaatagatt ataatcctta tagaccgatc gcacggtcta taaggattgg
     8401 agggaactta aatgatttca ttttttacaa aaactactga tatgatgaca tcaaaaaaaa
     8461 gatggactgc actagtagta ttagctgtta gtttgtttgt tgttacaatg gatatgacaa
     8521 tattaattat ggctttaccg gaattagtaa gagagttaga gccttctggt acccaacagt
     8581 tatggatagt tgatatatac tctcttgttt tagctggctt tataattcca ttgagtgcct
     8641 ttgctgataa atggggaaga aaaaaagcat tattaactgg atttgcttta tttggcctcg
     8701 tttcattagc tatatttttc gcagaaagtg cagagttcgt aatagctatt cgatttttac
     8761 ttggtattgc aggtgcttta ataatgccaa ctactctttc aatgataaga gtaatttttg
     8821 aaaaccctaa agaaagggcc actgcattag ctgtatggtc aatcgcttca tcgataggtg
     8881 ctgtttttgg accaattatc ggaggagctt tacttgagca attttcatgg cactcggcat
     8941 ttttaattaa tgtaccgttt gcgataatag cagttgtagc aggtttattt ttattaccag
     9001 agtctaagtt atcaaaagaa aagtctcact cgtgggatat tccttctaca attttatcaa
     9061 ttgcaggcat gattggactg gtatggagta tcaaagaatt ttcaaaagaa ggactagcag
     9121 atattattcc atgggttgta atagtattag caattaccat gatagtgata tttgttaaac
     9181 gtaatttatc aagttctgat ccaatgttag acgtaagact ttttaaaaag agatcatttt
     9241 cagctggtac aattgctgca tttatgacaa tttttgcaat ggcatctgtt ttgttattag
     9301 cttcacaatg gttacaggtt gtggaagaac tttctccttt taaagctggc ttatacctat
     9361 tacctatggc aataggagat atggtgtttg caccaattgc acccggatta gcggcgcgat
     9421 ttggaccgaa aatagtgtta ccttccggaa ttggaattgc agccattggc atgtttatta
     9481 tgtatttctt tggtcatcca ttatcatatt ctacaatggc tttagcatta attttagttg
     9541 gagctggtat ggcttcacta gcagttgcat ctgctctaat aatgttagaa acacctacat
     9601 caaaagcagg taatgcagct gctgttgaag agtctatgta tgaccttgga aatgtttttg
     9661 gtgtagcagt acttggtagc ctatcttcta tgctttatcg tgtattttta gatatttcat
     9721 ctttttcatc aaaaggtata gttggagatt tagctcatgt agctgaagaa tctgtagtgg
     9781 gcgctgtcga agtagctaaa gctacgggga taaaacagct tgcaaacgag gctgtaacat
     9841 catttaatga tgcttttgta gcaactgctt tagtaggtgg gattatcatg attatcattt
     9901 caatagttgt ctatttgtta attcccaaat cacttgatat aactaaacaa aaatagtact
     9961 aaacatatat ttttagatat tgtagattag tttattttta aaattagtaa tagttaaaaa
    10021 gtagaaccat taattttaaa tggttctact tttttattat taagtaaaat tataaaagac
    10081 aatttcttaa attaataata tgatatacct atatctaata atttattagt attaataaac
    10141 cttgaaagga tgattaactg atgaatagag cagatatttt taaaatattt gatgtagaac
    10201 ttaatataac tatattttta ggagcactag tagcaacttt catctatttt ataagttaca
    10261 tgaacaatgt aaatgaatta attacattat gcttaacatt agcttttcct gcatatttat
    10321 taaaaattag cagatcgttc gaggttattt tattagagga aaaaataaaa aatagtaaaa
    10381 tcaaaatctg tgacaaagat agaattgaac gagagatact ggtgaaaaag aacctaatag
    10441 atgaactatt gtttcctaat agagttatta aaaaatatac actgacaatt ttaatttaca
    10501 gtataggtat tattattctt ttactacttt gtaatatgtt aagttatgga attggtgaaa
    10561 atgtcaattt tatctggaga ttactagctt gttatgtaac gagtgtcaca gtgattatag
    10621 ttgccttgat tataatgata atgatatttg atcttataat tggattacta ttaaattttc
    10681 aaatattttt aaaaaataaa aagtatgatg attttttata attattcgat ttatataatg
    10741 aaaaattaaa tgattaaaca tagaaacctc aaaacaaacc atatttaata gagtttatgg
    10801 cgccttatac aaataaaggc tatgctgtta aagaattatg tagctatctt cagattaata
    10861 tggatgatgt ttatactatt ggcgatagaa aattgattta cctatgttta ataccctaga
    10921 taattcttct gataatttag tttttgttag agtttaacat aactgatgat tatcagaagt
    10981 ttgattcaca ttaaaaaggc acaacaagtt catgaacttg tgtgcctttt taattgcaat
    11041 tagttaccaa ataaacgact ccaaaatctc tttttaggtg gttctacctc cctattatca
    11101 ctggaatcgt tcttcgtttt ttcaacaaac ttatcctctt ttgttaattc atctgtactg
    11161 tcatgcttat tctcttcagt tatattttta tgatttactt cggttggttg acattctttt
    11221 tgttgttggt tgatattttg agattcttca gtaaaagttg cttcttgtgg attaatattc
    11281 tgtctattat tcatcgttgt atcaaaagaa tatttaagct gtctttcttc ttctaattgg
    11341 ctttctagtt tttgaatttt tttattgttt tctaaagata atatttgttg attctccaac
    11401 agtttattta atgtattaac attgctattt tgttgatcta attgtttagt tagattctca
    11461 atatattttt catcattttt agctctagtt tcaaagattt ctatttgctt ttctaattcg
    11521 tttacttttg tttttaatgt ctcagaacta tttgaattat tatgtatata cttttcaact
    11581 tttgcagtat tttcattaaa taattcattt tttactgatt ctttattatg agttgttttc
    11641 tctttcttag tgattttttc tataattttt aaataatcat tatctttaat ataattaatt
    11701 ccattttctt tttcaaaaga tatatttaat cttttagcat tattaacaat agtttgttta
    11761 gttacaccta attcatcagc aatcatttta atagttttca taacatcccc ccttaggtac
    11821 ctaatttagt acctaaattt taccacctaa caaaatttat ttcatcattt agtactaaat
    11881 ttactatcta ttaactacct aaatgcctaa ttatctatcc ttaaaatatt ttcgccactt
    11941 aattaggtgc taaaatagcg aattatacgt ttggtagttt taggtgtact tttaattaca
    12001 tttaaaactc tttatatacg ccattaaaag tgttaatatt actttataaa tattaaaaga
    12061 gtcgatgcta ttggcgtagc atcgactctc ggtaataaaa cgattcgcac tcgtttgttt
    12121 atatattttt ttgatacttg tattatatat atctaatcat ctaagtgcaa gcacaaaaca
    12181 tataacttac gtaaaaattg ttttattacc tcaatcccaa aatggaaatg aggtttttat
    12241 tatgcccaat tttgaaaaat ataatttatc acaagtaaaa actgaaagat tttatcaact
    12301 gcctaaatat ttatttgaag atgcatattt taagaaaatg tctgcagaag ccaaaattat
    12361 gtatgcgtta ttaaaagatc gttttgaatt atccctccaa aatgaatggg tagataaaaa
    12421 taataatatt tactttattt tcagtaataa acatttgtgt gaatacttag gttatgcaga
    12481 acaaaaaatt ataaaattaa aaaaagagtt aataaaattt aatttactaa ctcaagaacg
    12541 tgttggcctt aataaaccaa atagattata cctattaaaa cctaattatg acattgaagc
    12601 cagtcatatc aaggaacttc caaattcaca gttccagaac aatgaatttg gaagttctag
    12661 aactgtgaat ttaagtggtc aagaacttcc aaattcacag tctaatgata ctgattataa
    12721 tgacactgat tatattaaga ctaattataa tgatatgtat gatttgaatg ataggaaatt
    12781 aacatattct agtaatcata caaatcattc aaatcactac tattcaaaat tcaatgatga
    12841 agctttaaaa tttcaattac ttgaagaact accacaaagt attcaaagtt atttaagtaa
    12901 cttttctgta tctgaaatta aacttattaa atctgtgtta ttaaaagcca aaacatcctt
    12961 caacaattct attgatgcat attatttatt agaagatatg gaatttgaaa ttgttaatgt
    13021 tcttaagcgt tttaaagcta cattaattca aaaaaatgaa accgttgaag caatgcaagg
    13081 gtacttaatg aaatctctta aatctgaatt tgcagaagta catacgctta ataaacgacg
    13141 tgatcattta ccaatcactt ctttatttaa tcaataatac aacttaataa gccaacaatg
    13201 atccaattag agaatatctt taattagatg ttctcttaac tgtaagcgaa ctttattttc
    13261 aatgaaatgt aattaattaa gtaagtggga ctaaaacttt tcttttttag tcccattttt
    13321 ataaacaatt attattcatt gttactacta tcagcaattt tttcttttta gtgaaaccca
    13381 agtatttgta agcatgataa attacttctt tttatattta ttgggtttat ttgtattaat
    13441 gtagttggaa tacatcttta acaattcttc tgtttcatca ggacttaaat gctcacattc
    13501 aaaatacttt tcaagcattg caccttttcg aatcagtcta tgtgctcttt cttttctatc
    13561 taaatttttt
//
