LOCUS       NZ_CP073868            13570 bp    DNA     circular CON 17-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1279591 plasmid p1B1279591,
            complete sequence.
ACCESSION   NZ_CP073868
VERSION     NZ_CP073868.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699889
            Assembly: GCF_019329765.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 205x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019329765.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 06:59:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,677
            CDSs (total)                      :: 2,596
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 44 of 81
            Pseudo Genes (incomplete)         :: 43 of 81
            Pseudo Genes (internal stop)      :: 27 of 81
            Pseudo Genes (multiple problems)  :: 24 of 81
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073868.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1279591"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p1B1279591"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(13313..13570,1..12))
                     /locus_tag="KFV51_RS00005"
                     /old_locus_tag="KFV51_00005"
     CDS             complement(join(13313..13570,1..12))
                     /locus_tag="KFV51_RS00005"
                     /old_locus_tag="KFV51_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            221..2143
                     /locus_tag="KFV51_RS00010"
                     /old_locus_tag="KFV51_00010"
     CDS             221..2143
                     /locus_tag="KFV51_RS00010"
                     /old_locus_tag="KFV51_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            2417..2830
                     /locus_tag="KFV51_RS13460"
                     /old_locus_tag="KFV51_00015"
     CDS             2417..2830
                     /locus_tag="KFV51_RS13460"
                     /old_locus_tag="KFV51_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            2907..3140
                     /locus_tag="KFV51_RS00020"
                     /old_locus_tag="KFV51_00020"
     CDS             2907..3140
                     /locus_tag="KFV51_RS00020"
                     /old_locus_tag="KFV51_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(3189..3893)
                     /locus_tag="KFV51_RS00025"
                     /old_locus_tag="KFV51_00025"
     CDS             complement(3189..3893)
                     /locus_tag="KFV51_RS00025"
                     /old_locus_tag="KFV51_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(4044..4742)
                     /locus_tag="KFV51_RS00030"
                     /old_locus_tag="KFV51_00030"
     CDS             complement(4044..4742)
                     /locus_tag="KFV51_RS00030"
                     /old_locus_tag="KFV51_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            4906..5004
                     /locus_tag="KFV51_RS00035"
                     /old_locus_tag="KFV51_00035"
     CDS             4906..5004
                     /locus_tag="KFV51_RS00035"
                     /old_locus_tag="KFV51_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(5181..6092)
                     /locus_tag="KFV51_RS00040"
                     /old_locus_tag="KFV51_00040"
     CDS             complement(5181..6092)
                     /locus_tag="KFV51_RS00040"
                     /old_locus_tag="KFV51_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(6511..7071)
                     /locus_tag="KFV51_RS00045"
                     /old_locus_tag="KFV51_00045"
     CDS             complement(6511..7071)
                     /locus_tag="KFV51_RS00045"
                     /old_locus_tag="KFV51_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            complement(7322..7888)
                     /gene="qacR"
                     /locus_tag="KFV51_RS00050"
                     /old_locus_tag="KFV51_00050"
     ARG             complement(7326..7888)
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            8067..9611
                     /gene="qac"
                     /locus_tag="KFV51_RS00055"
                     /old_locus_tag="KFV51_00055"
     CDS             8067..9611
                     /gene="qac"
                     /locus_tag="KFV51_RS00055"
                     /old_locus_tag="KFV51_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            9816..10376
                     /locus_tag="KFV51_RS00060"
                     /old_locus_tag="KFV51_00060"
     CDS             9816..10376
                     /locus_tag="KFV51_RS00060"
                     /old_locus_tag="KFV51_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(10695..11456)
                     /locus_tag="KFV51_RS00070"
                     /old_locus_tag="KFV51_00070"
     CDS             complement(10695..11456)
                     /locus_tag="KFV51_RS00070"
                     /old_locus_tag="KFV51_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            11897..12832
                     /locus_tag="KFV51_RS00075"
                     /old_locus_tag="KFV51_00075"
     CDS             11897..12832
                     /locus_tag="KFV51_RS00075"
                     /old_locus_tag="KFV51_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            complement(13057..13296)
                     /locus_tag="KFV51_RS00080"
                     /old_locus_tag="KFV51_00080"
     CDS             complement(13057..13296)
                     /locus_tag="KFV51_RS00080"
                     /old_locus_tag="KFV51_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     ARG             8068..9608
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          11898..12832
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          222..2074
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 aattttagac attaaaaaac tcccttagtt tatctatata aagcatacca taattcattg
       61 ggtaagtcgc acatccatgg tataatataa ccagttctta agacactgca ctgcccattt
      121 acatgcaaat tgaaaattgg cataaaaatg ggcaaccata ctggttgatc attatagttc
      181 ccacaggggt aaaaaaacag aaaagagagg aggtaaaatc atgaataaag gttactttat
      241 gttgagagtg actaacatta atcgagcaac aaaaaatatc gttgcgtcag cgtcttatcg
      301 ttctgatgaa gcattatata gcgaacgtac agatgaaaaa attaagttta gaaatcatac
      361 tattaagcct gaaagcatga ttttaactcc tcaacatgca cccgaatgga ctaaagatag
      421 acagcgttta tggaatgaag ttgataaagt tgaaaagaat aatgcgaaaa caaaaaatcc
      481 tagattagca aaagaagtct tattaagttt gcctaatgat tttgatagag aggtacaaac
      541 tgaattaact aaagatttta taaaaagtga gtttgtagac aaaggaatgg ttgctgatat
      601 ttcaattcat agagatgata taaacaaccc acacgcccat gttctactta ctcaaagacc
      661 gtttaatgca gacggtactt gggggaaaaa gacaaaaacg agaacgcaat atgatgaaaa
      721 tggcaatgcg attttaaata aaaatggtaa taaagttaga aaacaagaac gatttgccga
      781 ctttgatttc aaagaagttc gtaagcattg ggaattaaaa ttaaatcaat actcagaacg
      841 tgaacaaagt ttaagacgat atgatagtcg ttcttttgaa gcgcaaggtt tagaaaaaat
      901 tgccgaaatt ccacttacaa gagaagaata tagattagaa caaaatgaaa gaaaacgctg
      961 tgaaaaagag ggtatcgagt accaacccgt tacttattac ggacagaaaa atgaacaaat
     1021 tagaagatat aatcgtggcg aagtatcaca attatttagt gataaagaga aagaacaagc
     1081 acaaaatgaa attagattat ttattcaaaa tgctaacgaa caagtaagta aaaatgaaca
     1141 aagttattct attttatttg atagatatca acgtgatgtt ggctattcag aggcgaaaga
     1201 aacaattaaa aatacgtttg acagtgcgtc tacctttggt cgtaaaattc aaaatgattt
     1261 acttaaaaat gaaattaaga gacgtcaatt aaaattctat gctaaaaatt atgatgaaaa
     1321 ttatcagttt agaaaagaaa ttgaaagtaa aggatatagt caacaagagt ttgttaatac
     1381 tgttgaaact aactataaag cgttactcaa tgagaagaat aaattagaaa gtagagagga
     1441 aaaacgtaaa gagatttatc aatcagctaa agatgtttat ctaactgaaa gtgaaaaaaa
     1501 tcatctcatt gttcagcaaa tgtaccctaa atctcatcat cattttagcc atgatgaaaa
     1561 agcatttatt gttgatgagg cacaaaaagg taatattata aaagcaactc atgttcaata
     1621 cctgttcaaa caaaataaca atattcctaa tgatgtattg ctagaagata cttataaaaa
     1681 agcgagtaaa gatattttct ttagtcaaaa acatcttaaa aatttagaaa aagatagctt
     1741 tgaatataac gttgagttgc aatttattaa ggaaaaagaa aaagaaattg atagttataa
     1801 agaacatatt gatgatgatt taagaataga tataggcgat aaaaactttg aaaaagtcaa
     1861 agaccaaaca cctaatgaaa aagttcaatt actcattaaa cttgagaaat ataatcatgt
     1921 cgatcaagaa gaagttatcg aaaaacatat taatcaagta gatagaaatg atcatgacaa
     1981 tcgtattgaa catgaaaatg aacaaactaa tcacattaat ttaccaacga taaatgctag
     2041 tgactttctt atgtactatg cgaacgaaac tgaaaagcaa aacaatatta atcatcagaa
     2101 aaagaagaag aaacaagaga gaaataacca aatgagaaga tagtaagcag ttgaaatcat
     2161 acaaagttca ttctttaata ataagaaact taacccaatg tggtaaatta gctttggttt
     2221 aaagaaaaca tttatatact tagataaggt acattctatg tattatcaca atgtatttta
     2281 aaacgtgtat aattatagaa aaaccttgat attatgcgat tgttcacata tttcagcata
     2341 aaaaatacta atgttcataa atatcgatag aaaaaattga gtttaaatat atcttaaagt
     2401 taaggagaaa aataatttgg aaacaaaaat tggacatcaa atacaaaagt taagaaaaag
     2461 taaaaacatg tctcaagaaa aattagctga aaagttaggg gtatcgagac attctatttc
     2521 taattgggaa agagaagtaa gtaatcccga cttaaaaaca attttagaaa tcactaaatt
     2581 atttaatgtt tctctaaatc aattaattaa aggagtggaa atcatgcaag ttaataaata
     2641 tgtttactct gcattagcat tttttctagg aggtttcggg gctcataagt tttatgttaa
     2701 gaaaaataaa aatgctcttc tttatttact attttgttgg acgggaatac cgggtgtgat
     2761 aggtatggta gaaggtgttt taacacttat gcgtccgagc gatagtgaaa acaatataga
     2821 aataaattaa accaaaaata aaaagccgat gtatcggatt ttttttagtg tacatgtgaa
     2881 tattaaaaat gatggagtga tttcacatga acacattcgt tttcggcgtg attacaaagc
     2941 gattagaaga aataggtttt aatgtacaca aggtgtttaa aatgcccgaa aaacaggcac
     3001 tatttgaaga tttgtaccat tatgtgagat acgttaaacc atgcaaggct gaactaacaa
     3061 tggtggcatt agatagtgat gaattgattc attactttat atgctgttac atggaacgtt
     3121 atgcttatct tttgaactaa tgataaaaaa gacgcctaat tttaggcgcc tttttaataa
     3181 tctactaact acttattata tctaatttta cttgctaact tagctactgt tttattgtcg
     3241 tagggtttaa tatagactgt ttcaatattc tcctctattg ctttttttgt tatcttattc
     3301 atttgactat aatcactatt ttgtgaattt tcccatttat aagggatatt attatataca
     3361 ttaattgttc catcaccatt actgctatat gttacagacc caccagctct cataggagct
     3421 tctaatttca caactttttc tttataaatt gcactatttt tggcttgtgg atttactttt
     3481 gagccttttg gtatttctgt aacataaaca tttttaatac cttttaaatc atttcttgta
     3541 gatattaact gataccaaac tcgtgcatat tcaatttctt tcgagttaaa attatttaag
     3601 taacttttat ttttatcagt agattgttgc ttcttattac tatcattatt tttttcttgt
     3661 tttttattat catttttttc tttattttgt ttattactac tttctgaatt atccgcacta
     3721 tcagatgtgt tagttttttc gtcaatttca tttgatgctt cattatcttt gttttctgag
     3781 gtgttagtat cactagaatt attatcattg tgatgttctg aatcgttatt attacaactc
     3841 actaaaatta ccgataaact aacaattaat ataggtaaaa tgattttttt catattacaa
     3901 ctccttttca gtatttcaaa ttagattatg ttaatatttt ttcagcaaga aaacgtaaat
     3961 tacatgagtt gaaaaatatg tattaaatat ataataaaat aaccacatgt tttacataaa
     4021 cacggtggtt attttatcaa tttttatatt gccttttttc tgaatattaa aagacttata
     4081 cctaatatta gtagtccaga agcgccaatt attgacaata tatcactttg gttgctttct
     4141 cctgtttctg gtaatacttg agagtttgaa tcttgatttt tcattccttc agaactcttg
     4201 tttttatttt cagttacagg tgtcaacaca ccttgaggtg tattaatagt tttattttct
     4261 tcattagcta tcttttgact ttcatttttt acaatttgat tatatttttc tgtggataca
     4321 ctattagggt taggaacatg actttctaat tcttggtatt cttcttgagt catacaatta
     4381 ggtatcaccc ctccgaaagg tcttttacct tgttgagttc tctcattatt ttgtatatca
     4441 gtactttgtg attgatattc attaaataca cttgaaactt tctcattata ctcgatttga
     4501 gttaattccc ctgaatcttt ctgtttagaa agttcactta tttgattttg tgcagattgt
     4561 gggagggatg aagtattgaa gtctacatta gcccaaccat ctccatttgt atctgcgcta
     4621 tttggtaagt tctctccgta tacggttttg gcaccatcct cagcattggc tgtcgctgaa
     4681 aaacctgtga atgttaatga gctagccaat aataaagttg gaagtaatga tcgttttttc
     4741 ataaaattat ctcctttaat agaatttgta ttttcattat cacatagata cattaaacat
     4801 aaaatgactt atcggaagtg catatgctta acaattcaca aatgatataa tataaaagtc
     4861 gtcgttctac ttctgtgctg gtttagcatg gaaggaggtg acaccatgtt cagcatcata
     4921 tttgtatcta tagtagctcc tattatagta ggggttataa tcacgttgtt ttcacattgg
     4981 ttgaataatc gtgataaaca ataaagcgac gacacagtaa taacaccaaa aagagcccga
     5041 taactactgg aatagttatc gggctcggtg tttaccatgt tcaacacata tttgttatct
     5101 aaattataac atcatagtgt ggaggaatgc aaaattgaat aattagtatg ttaagtctat
     5161 actatactga ttagtaagta ctaattatca tctttgttat tgttacccct ttcattggct
     5221 tttgaatcta attctttttg aaattttttg tgtgattttg aagaagtgtt taaaaagaaa
     5281 taggtcatat tatccatacg atgtcctttt ataaattcat tgataaattg tttttttgtc
     5341 atttttttgg ttgtattacg ttgtttttgc aatctaatga tttcttcttc aatgtatact
     5401 cttatcatca aatctaaact agatgtctga ttataagtag tgaataattt tggaactaat
     5461 aaagttttat taccatcaaa aagaaatgga gtgaaatcat aagtttccca tataccgtta
     5521 gtagagaaat aataatatgt cacagtttct ttagaaagaa acgatttcat ttctggaaat
     5581 gactgaatca tggatttatt aaaatcaatt ataacattca tgattatatt acttgttaaa
     5641 tctgataatt tatcatcacc aaaatttctg attaatggtg ctttttgagg tattgttaat
     5701 ccgtcatcat ttaagataat ggaaagaata tcttcattca taaatagatt aatcaattca
     5761 gatgctgtag taccttttcc tgtattacct ttctgagaaa aacctaagcg taaatggtgt
     5821 ggttctgaaa aatgagagaa taattttcta aattcattac gcttttgggt atcagaaaaa
     5881 tcactaagat gtatatcgat aattttttca aaaaaaacat caatttgctc ttgtgctctt
     5941 tgagccaaag tcgcaacttt tccaacttct ctattagata ttactttaat aaggtttggg
     6001 tctatatatt gaagagtgtc ttcttcaata gaaatattta aataatcagt tgatttagca
     6061 tcatgattga atttggtaca tactagtgtc actctatatc atccttctgt aagttttttg
     6121 tttacttcca atatagaatg atgtattatt gtaattgtcg atttacaata agttgttatt
     6181 ctaggtggaa gtttattgct tttgatgtat agatagactt aaccttttca ttatagcata
     6241 ctatagaaga aaatgcaagc aaaagcatat aaacaacaaa tgttgtttat atgcttttgc
     6301 ttttttgtta attggagagg acaaggtgaa tattaacagt caaaatccag ttatgtcggt
     6361 taatgaatcg atgaaaattt tcaaagctca agtaatgctt agaaatttaa agctattaag
     6421 cgagtggatt gttaaagggg actgaagtta aagacacttt ttaagaagat ttttcaatta
     6481 aagggtatat taaaacttta atttagtact ttatattttt cttgactctt taataatcct
     6541 atatacagta gaacgactca ctcctgtttg ttcagcaatt tcttcaccag acagcttttg
     6601 ttcatcatat aagaatttga tctctctttt cttatgatct ggtagtgaag gtcttccacc
     6661 ttttctccct cttgctcttg cagcttccaa acctttttta gttcgttcac ttaataaatt
     6721 agcttcaagt tcagcgaatg cactcatcat ggtaaagaac atcttgccca tcgcatcttt
     6781 cgttgacaca ttcatatcta taatatgtaa atcaatgcta ttttcatcca accattgaga
     6841 tagctcaatt aattgttttg ttgtacgtcc aagacgatct aatttataga caactagaat
     6901 atctccttca cgtaaataat ctaaacactt atcaagttcc gttcttttag ttttacgacc
     6961 actcgctttc tcgctaaaga tacgttcaca accatattcc tcaagtgtat caatctgtcc
     7021 atcaagactt tgatcttgtg ttgacacacg tgcataacca attttagcca tttgaacacc
     7081 acctttttta atcgttttat gggtacttac taatgaaata tagttctcga tgtaaataaa
     7141 cacgtctgtc agcatctaaa aactcatgat tttcgatatt tatgatacat tgattataac
     7201 acaagtttat agacttaaat atccaagaaa aacctcttaa tattaagtta agaggttttg
     7261 tgtcaaaaac gaacgtaatt tagaaataat atttattggt atttcaagaa tatatatata
     7321 attatttact aagtccattt aaaaaaattt gagagaactt gttcataagt ttaattcttt
     7381 cattaatatt ttgttcatgt gtaaaggtaa caattccatt aacagcgttt gctgctattt
     7441 tactaacagc attaacatca ttaatacacc attcgccatt taaattaccc tccttaaaaa
     7501 ttacatgata agcatctata tatttgtttt ctaatttatt cattttttca ttaatgctat
     7561 tagttttata atattcagtg taaaattcaa ttattgcgtt ttgaagtgga taatagtatt
     7621 cggttgttaa agatagttca ttatataaat aaaatttttc tctattagtt ttacatttga
     7681 tttgttcctt tttccactgt tcttgccatt tagattcttc tatatttaaa atttctaaaa
     7741 atagattttc ttttgtttta aagtgataat aaagattccc tttactactt tctgataatt
     7801 taacaatttc tccagtagta gtggcattat atccattttt tataaataat tcctttgcga
     7861 cacctagtat tttatctttc aagttcatca cgcctttaat gagtattaag tactatcaat
     7921 gatagcacaa aaaattcaaa gaataattgt ataacacaat acagccattt aaatttcgca
     7981 agattttttg ttgtaatatg taaaaaaaat agattataat ccttatagac cgatcgcacg
     8041 gtctataagg attggaggga acttaaatga tttcattttt tacaaaaact actgatatga
     8101 tgacatcaaa aaaaagatgg actgcactag tagtattagc tgttagtttg tttgttgtta
     8161 caatggatat gacaatatta attatggctt taccggaatt agtaagagag ttagagcctt
     8221 ctggtaccca acagttatgg atagttgata tatactctct tgttttagct ggctttataa
     8281 ttccattgag tgcctttgct gataaatggg gaagaaaaaa agcattatta actggatttg
     8341 ctttatttgg cctcgtttca ttagctatat ttttcgcaga aagtgcagag ttcgtaatag
     8401 ctattcgatt tttacttggt attgcaggtg ctttaataat gccaactact ctttcaatga
     8461 taagagtaat ttttgaaaac cctaaagaaa gggccactgc attagctgta tggtcaatcg
     8521 cttcatcgat aggtgctgtt tttggaccaa ttatcggagg agctttactt gagcaatttt
     8581 catggcactc ggcattttta attaatgtac cgtttgcgat aatagcagtt gtagcaggtt
     8641 tatttttatt accagagtct aagttatcaa aagaaaagtc tcactcgtgg gatattcctt
     8701 ctacaatttt atcaattgca ggcatgattg gactggtatg gagtatcaaa gaattttcaa
     8761 aagaaggact agcagatatt attccatggg ttgtaatagt attagcaatt accatgatag
     8821 tgatatttgt taaacgtaat ttatcaagtt ctgatccaat gttagacgta agacttttta
     8881 aaaagagatc attttcagct ggtacaattg ctgcatttat gacaattttt gcaatggcat
     8941 ctgttttgtt attagcttca caatggttac aggttgtgga agaactttct ccttttaaag
     9001 ctggcttata cctattacct atggcaatag gagatatggt gtttgcacca attgcacccg
     9061 gattagcggc gcgatttgga ccgaaaatag tgttaccttc cggaattgga attgcagcca
     9121 ttggcatgtt tattatgtat ttctttggtc atccattatc atattctaca atggctttag
     9181 cattaatttt agttggagct ggtatggctt cactagcagt tgcatctgct ctaataatgt
     9241 tagaaacacc tacatcaaaa gcaggtaatg cagctgctgt tgaagagtct atgtatgacc
     9301 ttggaaatgt ttttggtgta gcagtacttg gtagcctatc ttctatgctt tatcgtgtat
     9361 ttttagatat ttcatctttt tcatcaaaag gtatagttgg agatttagct catgtagctg
     9421 aagaatctgt agtgggcgct gtcgaagtag ctaaagctac ggggataaaa cagcttgcaa
     9481 acgaggctgt aacatcattt aatgatgctt ttgtagcaac tgctttagta ggtgggatta
     9541 tcatgattat catttcaata gttgtctatt tgttaattcc caaatcactt gatataacta
     9601 aacaaaaata gtactaaaca tatattttta gatattgtag attagtttat ttttaaaatt
     9661 agtaatagtt aaaaagtaga accattaatt ttaaatggtt ctactttttt attattaagt
     9721 aaaattataa aagacaattt cttaaattaa taatatgata tacctatatc taataattta
     9781 ttagtattaa taaaccttga aaggatgatt aactgatgaa tagagcagat atttttaaaa
     9841 tatttgatgt agaacttaat ataactatat ttttaggagc actagtagca actttcatct
     9901 attttataag ttacatgaac aatgtaaatg aattaattac attatgctta acattagctt
     9961 ttcctgcata tttattaaaa attagcagat cgttcgaggt tattttatta gaggaaaaaa
    10021 taaaaaatag taaaatcaaa atctgtgaca aagatagaat tgaacgagag atactggtga
    10081 aaaagaacct aatagatgaa ctattgtttc ctaatagagt tattaaaaaa tatacactga
    10141 caattttaat ttacagtata ggtattatta ttcttttact actttgtaat atgttaagtt
    10201 atggaattgg tgaaaatgtc aattttatct ggagattact agcttgttat gtaacgagtg
    10261 tcacagtgat tatagttgcc ttgattataa tgataatgat atttgatctt ataattggat
    10321 tactattaaa ttttcaaata tttttaaaaa ataaaaagta tgatgatttt ttataattat
    10381 tcgatttata taatgaaaaa ttaaatgatt aaacatagaa acctcaaaac aaaccatatt
    10441 taatagagtt tatggcgcct tatacaaata aaggctatgc tgttaaagaa ttatgtagct
    10501 atcttcagat taatatggat gatgtttata ctattggcga tagaaaattg atttacctat
    10561 gtttaatacc ctagataatt cttctgataa tttagttttt gttagagttt aacataactg
    10621 atgattatca gaagtttgat tcacattaaa aaggcacaac aagttcatga acttgtgtgc
    10681 ctttttaatt gcaattagtt accaaataaa cgactccaaa atctcttttt aggtggttct
    10741 acctccctat tatcactgga atcgttcttc gttttttcaa caaacttatc ctcttttgtt
    10801 aattcatctg tactgtcatg cttattctct tcagttatat ttttatgatt tacttcggtt
    10861 ggttgacatt ctttttgttg ttggttgata ttttgagatt cttcagtaaa agttgcttct
    10921 tgtggattaa tattctgtct attattcatc gttgtatcaa aagaatattt aagctgtctt
    10981 tcttcttcta attggctttc tagtttttga atttttttat tgttttctaa agataatatt
    11041 tgttgattct ccaacagttt atttaatgta ttaacattgc tattttgttg atctaattgt
    11101 ttagttagat tctcaatata tttttcatca tttttagctc tagtttcaaa gatttctatt
    11161 tgcttttcta attcgtttac ttttgttttt aatgtctcag aactatttga attattatgt
    11221 atatactttt caacttttgc agtattttca ttaaataatt cattttttac tgattcttta
    11281 ttatgagttg ttttctcttt cttagtgatt ttttctataa tttttaaata atcattatct
    11341 ttaatataat taattccatt ttctttttca aaagatatat ttaatctttt agcattatta
    11401 acaatagttt gtttagttac acctaattca tcagcaatca ttttaatagt tttcataaca
    11461 tcccccctta ggtacctaat ttagtaccta aattttacca cctaacaaaa tttatttcat
    11521 catttagtac taaatttact atctattaac tacctaaatg cctaattatc tatccttaaa
    11581 atattttcgc cacttaatta ggtgctaaaa tagcgaatta tacgtttggt agttttaggt
    11641 gtacttttaa ttacatttaa aactctttat atacgccatt aaaagtgtta atattacttt
    11701 ataaatatta aaagagtcga tgctattggc gtagcatcga ctctcggtaa taaaacgatt
    11761 cgcactcgtt tgtttatata tttttttgat acttgtatta tatatatcta atcatctaag
    11821 tgcaagcaca aaacatataa cttacgtaaa aattgtttta ttacctcaat cccaaaatgg
    11881 aaatgaggtt tttattatgc ccaattttga aaaatataat ttatcacaag taaaaactga
    11941 aagattttat caactgccta aatatttatt tgaagatgca tattttaaga aaatgtctgc
    12001 agaagccaaa attatgtatg cgttattaaa agatcgtttt gaattatccc tccaaaatga
    12061 atgggtagat aaaaataata atatttactt tattttcagt aataaacatt tgtgtgaata
    12121 cttaggttat gcagaacaaa aaattataaa attaaaaaaa gagttaataa aatttaattt
    12181 actaactcaa gaacgtgttg gccttaataa accaaataga ttatacctat taaaacctaa
    12241 ttatgacatt gaagccagtc atatcaagga acttccaaat tcacagttcc agaacaatga
    12301 atttggaagt tctagaactg tgaatttaag tggtcaagaa cttccaaatt cacagtctaa
    12361 tgatactgat tataatgaca ctgattatat taagactaat tataatgata tgtatgattt
    12421 gaatgatagg aaattaacat attctagtaa tcatacaaat cattcaaatc actactattc
    12481 aaaattcaat gatgaagctt taaaatttca attacttgaa gaactaccac aaagtattca
    12541 aagttattta agtaactttt ctgtatctga aattaaactt attaaatctg tgttattaaa
    12601 agccaaaaca tccttcaaca attctattga tgcatattat ttattagaag atatggaatt
    12661 tgaaattgtt aatgttctta agcgttttaa agctacatta attcaaaaaa atgaaaccgt
    12721 tgaagcaatg caagggtact taatgaaatc tcttaaatct gaatttgcag aagtacatac
    12781 gcttaataaa cgacgtgatc atttaccaat cacttcttta tttaatcaat aatacaactt
    12841 aataagccaa caatgatcca attagagaat atctttaatt agatgttctc ttaactgtaa
    12901 gcgaacttta ttttcaatga aatgtaatta attaagtaag tgggactaaa acttttcttt
    12961 tttagtccca tttttataaa caattattat tcattgttac tactatcagc aattttttct
    13021 ttttagtgaa acccaagtat ttgtaagcat gataaattac ttctttttat atttattggg
    13081 tttatttgta ttaatgtagt tggaatacat ctttaacaat tcttctgttt catcaggact
    13141 taaatgctca cattcaaaat acttttcaag cattgcacct tttcgaatca gtctatgtgc
    13201 tctttctttt ctatctaaat tttttaatct atgctttctt aattctgctt tttcaatttc
    13261 agcatttatt tttttgagat gtttaaaatc attcatcgta tcacttacta ttctagttaa
    13321 ttttgtcttc tgttaaattt tccttgttct cgttgctgct attttgttcg atatctttat
    13381 taaattctcc aatgacttta tccataattt tatacaattc atcactagag ttgatcatat
    13441 ttaaatcaag tttatttaaa aaataaccac caattttatt agatagttgt tcttcttgtt
    13501 ttttcttttg ctctttcagt tgtgcaattt gtttttctaa ttgacttaat tttgtaatat
    13561 ctgtttgttt
//
