LOCUS       NZ_CP073866            13570 bp    DNA     circular CON 17-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1276912 plasmid p1B1276912,
            complete sequence.
ACCESSION   NZ_CP073866
VERSION     NZ_CP073866.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699888
            Assembly: GCF_019329745.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 349x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019329745.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 06:59:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,763
            CDSs (total)                      :: 2,682
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 82 of 124
            Pseudo Genes (incomplete)         :: 46 of 124
            Pseudo Genes (internal stop)      :: 46 of 124
            Pseudo Genes (multiple problems)  :: 41 of 124
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073866.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1276912"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p1B1276912"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(11890..13570,1..242))
                     /locus_tag="KFV35_RS13570"
                     /old_locus_tag="KFV35_13620"
     CDS             complement(join(11890..13570,1..242))
                     /locus_tag="KFV35_RS13570"
                     /old_locus_tag="KFV35_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            451..720
                     /locus_tag="KFV35_RS13575"
                     /old_locus_tag="KFV35_13625"
     CDS             451..720
                     /locus_tag="KFV35_RS13575"
                     /old_locus_tag="KFV35_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            737..976
                     /locus_tag="KFV35_RS13580"
                     /old_locus_tag="KFV35_13630"
     CDS             737..976
                     /locus_tag="KFV35_RS13580"
                     /old_locus_tag="KFV35_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(1201..2136)
                     /locus_tag="KFV35_RS13585"
                     /old_locus_tag="KFV35_13635"
     CDS             complement(1201..2136)
                     /locus_tag="KFV35_RS13585"
                     /old_locus_tag="KFV35_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            2577..3338
                     /locus_tag="KFV35_RS13590"
                     /old_locus_tag="KFV35_13640"
     CDS             2577..3338
                     /locus_tag="KFV35_RS13590"
                     /old_locus_tag="KFV35_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            complement(3657..4217)
                     /locus_tag="KFV35_RS13600"
                     /old_locus_tag="KFV35_13650"
     CDS             complement(3657..4217)
                     /locus_tag="KFV35_RS13600"
                     /old_locus_tag="KFV35_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(4422..5966)
                     /gene="qac"
                     /locus_tag="KFV35_RS13605"
                     /old_locus_tag="KFV35_13655"
     CDS             complement(4422..5966)
                     /gene="qac"
                     /locus_tag="KFV35_RS13605"
                     /old_locus_tag="KFV35_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            6145..6711
                     /gene="qacR"
                     /locus_tag="KFV35_RS13610"
                     /old_locus_tag="KFV35_13660"
     ARG             6146..6708
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6962..7522
                     /locus_tag="KFV35_RS13615"
                     /old_locus_tag="KFV35_13665"
     CDS             6962..7522
                     /locus_tag="KFV35_RS13615"
                     /old_locus_tag="KFV35_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            7941..8852
                     /locus_tag="KFV35_RS13620"
                     /old_locus_tag="KFV35_13670"
     CDS             7941..8852
                     /locus_tag="KFV35_RS13620"
                     /old_locus_tag="KFV35_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(9029..9127)
                     /locus_tag="KFV35_RS13625"
                     /old_locus_tag="KFV35_13675"
     CDS             complement(9029..9127)
                     /locus_tag="KFV35_RS13625"
                     /old_locus_tag="KFV35_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            9291..9989
                     /locus_tag="KFV35_RS13630"
                     /old_locus_tag="KFV35_13680"
     CDS             9291..9989
                     /locus_tag="KFV35_RS13630"
                     /old_locus_tag="KFV35_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            10140..10844
                     /locus_tag="KFV35_RS13635"
                     /old_locus_tag="KFV35_13685"
     CDS             10140..10844
                     /locus_tag="KFV35_RS13635"
                     /old_locus_tag="KFV35_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(10893..11126)
                     /locus_tag="KFV35_RS13640"
                     /old_locus_tag="KFV35_13690"
     CDS             complement(10893..11126)
                     /locus_tag="KFV35_RS13640"
                     /old_locus_tag="KFV35_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(11203..11616)
                     /locus_tag="KFV35_RS13935"
                     /old_locus_tag="KFV35_13695"
     CDS             complement(11203..11616)
                     /locus_tag="KFV35_RS13935"
                     /old_locus_tag="KFV35_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     ARG             complement(4426..5966)
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          complement(1202..2136)
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11960..13569)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 ttattctttt caactttatc aacttcattc cataaacgct gtctatcttt agtccattcg
       61 ggtgcatgtt gaggagttaa aatcatgctt tcaggcttaa tagtatgatt tctaaactta
      121 attttttcat ctgtacgttc gctatataat gcttcatcag aacgataaga cgctgacgca
      181 acgatatttt ttgttgctcg attaatgtta gtcactctca acataaagta acctttattc
      241 atgattttac ctcctctctt ttctgttttt ttacccctgt gggaactata atgatcaacc
      301 agtatggttg cccattttta tgccaatttt caatttgcat gtaaatgggc agtgcagtgt
      361 cttaagaact ggttatatta taccatggat gtgcgactta cccaatgaat tatggtatgc
      421 tttatataga taaactaagg gagtttttta atgtctaaaa ttaaacaaac agatattaca
      481 aaattaagtc aattagaaaa acaaattgca caactgaaag agcaaaagaa aaaacaagaa
      541 gaacaactat ctaataaaat tggtggttat tttttaaata aacttgattt aaatatgatc
      601 aactctagtg atgaattgta taaaattatg gataaagtca ttggagaatt taataaagat
      661 atcgaacaaa atagcagcaa cgagaacaag gaaaatttaa cagaagacaa aattaactag
      721 aatagtaagt gatacgatga atgattttaa acatctcaaa aaaataaatg ctgaaattga
      781 aaaagcagaa ttaagaaagc atagattaaa aaatttagat agaaaagaaa gagcacatag
      841 actgattcga aaaggtgcaa tgcttgaaaa gtattttgaa tgtgagcatt taagtcctga
      901 tgaaacagaa gaattgttaa agatgtattc caactacatt aatacaaata aacccaataa
      961 atataaaaag aagtaattta tcatgcttac aaatacttgg gtttcactaa aaagaaaaaa
     1021 ttgctgatag tagtaacaat gaataataat tgtttataaa aatgggacta aaaaagaaaa
     1081 gttttagtcc cacttactta attaattaca tttcattgaa aataaagttc gcttacagtt
     1141 aagagaacat ctaattaaag atattctcta attggatcat tgttggctta ttaagttgta
     1201 ttattgatta aataaagaag tgattggtaa atgatcacgt cgtttattaa gcgtatgtac
     1261 ttctgcaaat tcagatttaa gagatttcat taagtaccct tgcattgctt caacggtttc
     1321 atttttttga attaatgtag ctttaaaacg cttaagaaca ttaacaattt caaattccat
     1381 atcttctaat aaataatatg catcaataga attgttgaag gatgttttgg cttttaataa
     1441 cacagattta ataagtttaa tttcagatac agaaaagtta cttaaataac tttgaatact
     1501 ttgtggtagt tcttcaagta attgaaattt taaagcttca tcattgaatt ttgaatagta
     1561 gtgatttgaa tgatttgtat gattactaga atatgttaat ttcctatcat tcaaatcata
     1621 catatcatta taattagtct taatataatc agtgtcatta taatcagtat cattagactg
     1681 tgaatttgga agttcttgac cacttaaatt cacagttcta gaacttccaa attcattgtt
     1741 ctggaactgt gaatttggaa gttccttgat atgactggct tcaatgtcat aattaggttt
     1801 taataggtat aatctatttg gtttattaag gccaacacgt tcttgagtta gtaaattaaa
     1861 ttttattaac tcttttttta attttataat tttttgttct gcataaccta agtattcaca
     1921 caaatgttta ttactgaaaa taaagtaaat attattattt ttatctaccc attcattttg
     1981 gagggataat tcaaaacgat cttttaataa cgcatacata attttggctt ctgcagacat
     2041 tttcttaaaa tatgcatctt caaataaata tttaggcagt tgataaaatc tttcagtttt
     2101 tacttgtgat aaattatatt tttcaaaatt gggcataata aaaacctcat ttccattttg
     2161 ggattgaggt aataaaacaa tttttacgta agttatatgt tttgtgcttg cacttagatg
     2221 attagatata tataatacaa gtatcaaaaa aatatataaa caaacgagtg cgaatcgttt
     2281 tattaccgag agtcgatgct acgccaatag catcgactct tttaatattt ataaagtaat
     2341 attaacactt ttaatggcgt atataaagag ttttaaatgt aattaaaagt acacctaaaa
     2401 ctaccaaacg tataattcgc tattttagca cctaattaag tggcgaaaat attttaagga
     2461 tagataatta ggcatttagg tagttaatag atagtaaatt tagtactaaa tgatgaaata
     2521 aattttgtta ggtggtaaaa tttaggtact aaattaggta cctaaggggg gatgttatga
     2581 aaactattaa aatgattgct gatgaattag gtgtaactaa acaaactatt gttaataatg
     2641 ctaaaagatt aaatatatct tttgaaaaag aaaatggaat taattatatt aaagataatg
     2701 attatttaaa aattatagaa aaaatcacta agaaagagaa aacaactcat aataaagaat
     2761 cagtaaaaaa tgaattattt aatgaaaata ctgcaaaagt tgaaaagtat atacataata
     2821 attcaaatag ttctgagaca ttaaaaacaa aagtaaacga attagaaaag caaatagaaa
     2881 tctttgaaac tagagctaaa aatgatgaaa aatatattga gaatctaact aaacaattag
     2941 atcaacaaaa tagcaatgtt aatacattaa ataaactgtt ggagaatcaa caaatattat
     3001 ctttagaaaa caataaaaaa attcaaaaac tagaaagcca attagaagaa gaaagacagc
     3061 ttaaatattc ttttgataca acgatgaata atagacagaa tattaatcca caagaagcaa
     3121 cttttactga agaatctcaa aatatcaacc aacaacaaaa agaatgtcaa ccaaccgaag
     3181 taaatcataa aaatataact gaagagaata agcatgacag tacagatgaa ttaacaaaag
     3241 aggataagtt tgttgaaaaa acgaagaacg attccagtga taatagggag gtagaaccac
     3301 ctaaaaagag attttggagt cgtttatttg gtaactaatt gcaattaaaa aggcacacaa
     3361 gttcatgaac ttgttgtgcc tttttaatgt gaatcaaact tctgataatc atcagttatg
     3421 ttaaactcta acaaaaacta aattatcaga agaattatct agggtattaa acataggtaa
     3481 atcaattttc tatcgccaat agtataaaca tcatccatat taatctgaag atagctacat
     3541 aattctttaa cagcatagcc tttatttgta taaggcgcca taaactctat taaatatggt
     3601 ttgttttgag gtttctatgt ttaatcattt aatttttcat tatataaatc gaataattat
     3661 aaaaaatcat catacttttt attttttaaa aatatttgaa aatttaatag taatccaatt
     3721 ataagatcaa atatcattat cattataatc aaggcaacta taatcactgt gacactcgtt
     3781 acataacaag ctagtaatct ccagataaaa ttgacatttt caccaattcc ataacttaac
     3841 atattacaaa gtagtaaaag aataataata cctatactgt aaattaaaat tgtcagtgta
     3901 tattttttaa taactctatt aggaaacaat agttcatcta ttaggttctt tttcaccagt
     3961 atctctcgtt caattctatc tttgtcacag attttgattt tactattttt tattttttcc
     4021 tctaataaaa taacctcgaa cgatctgcta atttttaata aatatgcagg aaaagctaat
     4081 gttaagcata atgtaattaa ttcatttaca ttgttcatgt aacttataaa atagatgaaa
     4141 gttgctacta gtgctcctaa aaatatagtt atattaagtt ctacatcaaa tattttaaaa
     4201 atatctgctc tattcatcag ttaatcatcc tttcaaggtt tattaatact aataaattat
     4261 tagatatagg tatatcatat tattaattta agaaattgtc ttttataatt ttacttaata
     4321 ataaaaaagt agaaccattt aaaattaatg gttctacttt ttaactatta ctaattttaa
     4381 aaataaacta atctacaata tctaaaaata tatgtttagt actatttttg tttagttata
     4441 tcaagtgatt tgggaattaa caaatagaca actattgaaa tgataatcat gataatccca
     4501 cctactaaag cagttgctac aaaagcatca ttaaatgatg ttacagcctc gtttgcaagc
     4561 tgttttatcc ccgtagcttt agctacttcg acagcgccca ctacagattc ttcagctaca
     4621 tgagctaaat ctccaactat accttttgat gaaaaagatg aaatatctaa aaatacacga
     4681 taaagcatag aagataggct accaagtact gctacaccaa aaacatttcc aaggtcatac
     4741 atagactctt caacagcagc tgcattacct gcttttgatg taggtgtttc taacattatt
     4801 agagcagatg caactgctag tgaagccata ccagctccaa ctaaaattaa tgctaaagcc
     4861 attgtagaat atgataatgg atgaccaaag aaatacataa taaacatgcc aatggctgca
     4921 attccaattc cggaaggtaa cactattttc ggtccaaatc gcgccgctaa tccgggtgca
     4981 attggtgcaa acaccatatc tcctattgcc ataggtaata ggtataagcc agctttaaaa
     5041 ggagaaagtt cttccacaac ctgtaaccat tgtgaagcta ataacaaaac agatgccatt
     5101 gcaaaaattg tcataaatgc agcaattgta ccagctgaaa atgatctctt tttaaaaagt
     5161 cttacgtcta acattggatc agaacttgat aaattacgtt taacaaatat cactatcatg
     5221 gtaattgcta atactattac aacccatgga ataatatctg ctagtccttc ttttgaaaat
     5281 tctttgatac tccataccag tccaatcatg cctgcaattg ataaaattgt agaaggaata
     5341 tcccacgagt gagacttttc ttttgataac ttagactctg gtaataaaaa taaacctgct
     5401 acaactgcta ttatcgcaaa cggtacatta attaaaaatg ccgagtgcca tgaaaattgc
     5461 tcaagtaaag ctcctccgat aattggtcca aaaacagcac ctatcgatga agcgattgac
     5521 catacagcta atgcagtggc cctttcttta gggttttcaa aaattactct tatcattgaa
     5581 agagtagttg gcattattaa agcacctgca ataccaagta aaaatcgaat agctattacg
     5641 aactctgcac tttctgcgaa aaatatagct aatgaaacga ggccaaataa agcaaatcca
     5701 gttaataatg ctttttttct tccccattta tcagcaaagg cactcaatgg aattataaag
     5761 ccagctaaaa caagagagta tatatcaact atccataact gttgggtacc agaaggctct
     5821 aactctctta ctaattccgg taaagccata attaatattg tcatatccat tgtaacaaca
     5881 aacaaactaa cagctaatac tactagtgca gtccatcttt tttttgatgt catcatatca
     5941 gtagtttttg taaaaaatga aatcatttaa gttccctcca atccttatag accgtgcgat
     6001 cggtctataa ggattataat ctattttttt tacatattac aacaaaaaat cttgcgaaat
     6061 ttaaatggct gtattgtgtt atacaattat tctttgaatt ttttgtgcta tcattgatag
     6121 tacttaatac tcattaaagg cgtgatgaac ttgaaagata aaatactagg tgtcgcaaag
     6181 gaattattta taaaaaatgg atataatgcc actactactg gagaaattgt taaattatca
     6241 gaaagtagta aagggaatct ttattatcac tttaaaacaa aagaaaatct atttttagaa
     6301 attttaaata tagaagaatc taaatggcaa gaacagtgga aaaaggaaca aatcaaatgt
     6361 aaaactaata gagaaaaatt ttatttatat aatgaactat ctttaacaac cgaatactat
     6421 tatccacttc aaaacgcaat aattgaattt tacactgaat attataaaac taatagcatt
     6481 aatgaaaaaa tgaataaatt agaaaacaaa tatatagatg cttatcatgt aatttttaag
     6541 gagggtaatt taaatggcga atggtgtatt aatgatgtta atgctgttag taaaatagca
     6601 gcaaacgctg ttaatggaat tgttaccttt acacatgaac aaaatattaa tgaaagaatt
     6661 aaacttatga acaagttctc tcaaattttt ttaaatggac ttagtaaata attatatata
     6721 tattcttgaa ataccaataa atattatttc taaattacgt tcgtttttga cacaaaacct
     6781 cttaacttaa tattaagagg tttttcttgg atatttaagt ctataaactt gtgttataat
     6841 caatgtatca taaatatcga aaatcatgag tttttagatg ctgacagacg tgtttattta
     6901 catcgagaac tatatttcat tagtaagtac ccataaaacg attaaaaaag gtggtgttca
     6961 aatggctaaa attggttatg cacgtgtgtc aacacaagat caaagtcttg atggacagat
     7021 tgatacactt gaggaatatg gttgtgaacg tatctttagc gagaaagcga gtggtcgtaa
     7081 aactaaaaga acggaacttg ataagtgttt agattattta cgtgaaggag atattctagt
     7141 tgtctataaa ttagatcgtc ttggacgtac aacaaaacaa ttaattgagc tatctcaatg
     7201 gttggatgaa aatagcattg atttacatat tatagatatg aatgtgtcaa cgaaagatgc
     7261 gatgggcaag atgttcttta ccatgatgag tgcattcgct gaacttgaag ctaatttatt
     7321 aagtgaacga actaaaaaag gtttggaagc tgcaagagca agagggagaa aaggtggaag
     7381 accttcacta ccagatcata agaaaagaga gatcaaattc ttatatgatg aacaaaagct
     7441 gtctggtgaa gaaattgctg aacaaacagg agtgagtcgt tctactgtat ataggattat
     7501 taaagagtca agaaaaatat aaagtactaa attaaagttt taatataccc tttaattgaa
     7561 aaatcttctt aaaaagtgtc tttaacttca gtccccttta acaatccact cgcttaatag
     7621 ctttaaattt ctaagcatta cttgagcttt gaaaattttc atcgattcat taaccgacat
     7681 aactggattt tgactgttaa tattcacctt gtcctctcca attaacaaaa aagcaaaagc
     7741 atataaacaa catttgttgt ttatatgctt ttgcttgcat tttcttctat agtatgctat
     7801 aatgaaaagg ttaagtctat ctatacatca aaagcaataa acttccacct agaataacaa
     7861 cttattgtaa atcgacaatt acaataatac atcattctat attggaagta aacaaaaaac
     7921 ttacagaagg atgatataga gtgacactag tatgtaccaa attcaatcat gatgctaaat
     7981 caactgatta tttaaatatt tctattgaag aagacactct tcaatatata gacccaaacc
     8041 ttattaaagt aatatctaat agagaagttg gaaaagttgc gactttggct caaagagcac
     8101 aagagcaaat tgatgttttt tttgaaaaaa ttatcgatat acatcttagt gatttttctg
     8161 atacccaaaa gcgtaatgaa tttagaaaat tattctctca tttttcagaa ccacaccatt
     8221 tacgcttagg tttttctcag aaaggtaata caggaaaagg tactacagca tctgaattga
     8281 ttaatctatt tatgaatgaa gatattcttt ccattatctt aaatgatgac ggattaacaa
     8341 tacctcaaaa agcaccatta atcagaaatt ttggtgatga taaattatca gatttaacaa
     8401 gtaatataat catgaatgtt ataattgatt ttaataaatc catgattcag tcatttccag
     8461 aaatgaaatc gtttctttct aaagaaactg tgacatatta ttatttctct actaacggta
     8521 tatgggaaac ttatgatttc actccatttc tttttgatgg taataaaact ttattagttc
     8581 caaaattatt cactacttat aatcagacat ctagtttaga tttgatgata agagtataca
     8641 ttgaagaaga aatcattaga ttgcaaaaac aacgtaatac aaccaaaaaa atgacaaaaa
     8701 aacaatttat caatgaattt ataaaaggac atcgtatgga taatatgacc tatttctttt
     8761 taaacacttc ttcaaaatca cacaaaaaat ttcaaaaaga attagattca aaagccaatg
     8821 aaaggggtaa caataacaaa gatgataatt agtacttact aatcagtata gtatagactt
     8881 aacatactaa ttattcaatt ttgcattcct ccacactatg atgttataat ttagataaca
     8941 aatatgtgtt gaacatggta aacaccgagc ccgataacta ttccagtagt tatcgggctc
     9001 tttttggtgt tattactgtg tcgtcgcttt attgtttatc acgattattc aaccaatgtg
     9061 aaaacaacgt gattataacc cctactataa taggagctac tatagataca aatatgatgc
     9121 tgaacatggt gtcacctcct tccatgctaa accagcacag aagtagaacg acgactttta
     9181 tattatatca tttgtgaatt gttaagcata tgcacttccg ataagtcatt ttatgtttaa
     9241 tgtatctatg tgataatgaa aatacaaatt ctattaaagg agataatttt atgaaaaaac
     9301 gatcattact tccaacttta ttattggcta gctcattaac attcacaggt ttttcagcga
     9361 cagccaatgc tgaggatggt gccaaaaccg tatacggaga gaacttacca aatagcgcag
     9421 atacaaatgg agatggttgg gctaatgtag acttcaatac ttcatccctc ccacaatctg
     9481 cacaaaatca aataagtgaa ctttctaaac agaaagattc aggggaatta actcaaatcg
     9541 agtataatga gaaagtttca agtgtattta atgaatatca atcacaaagt actgatatac
     9601 aaaataatga gagaactcaa caaggtaaaa gacctttcgg aggggtgata cctaattgta
     9661 tgactcaaga agaataccaa gaattagaaa gtcatgttcc taaccctaat agtgtatcca
     9721 cagaaaaata taatcaaatt gtaaaaaatg aaagtcaaaa gatagctaat gaagaaaata
     9781 aaactattaa tacacctcaa ggtgtgttga cacctgtaac tgaaaataaa aacaagagtt
     9841 ctgaaggaat gaaaaatcaa gattcaaact ctcaagtatt accagaaaca ggagaaagca
     9901 accaaagtga tatattgtca ataattggcg cttctggact actaatatta ggtataagtc
     9961 ttttaatatt cagaaaaaag gcaatataaa aattgataaa ataaccaccg tgtttatgta
    10021 aaacatgtgg ttattttatt atatatttaa tacatatttt tcaactcatg taatttacgt
    10081 tttcttgctg aaaaaatatt aacataatct aatttgaaat actgaaaagg agttgtaata
    10141 tgaaaaaaat cattttacct atattaattg ttagtttatc ggtaatttta gtgagttgta
    10201 ataataacga ttcagaacat cacaatgata ataattctag tgatactaac acctcagaaa
    10261 acaaagataa tgaagcatca aatgaaattg acgaaaaaac taacacatct gatagtgcgg
    10321 ataattcaga aagtagtaat aaacaaaata aagaaaaaaa tgataataaa aaacaagaaa
    10381 aaaataatga tagtaataag aagcaacaat ctactgataa aaataaaagt tacttaaata
    10441 attttaactc gaaagaaatt gaatatgcac gagtttggta tcagttaata tctacaagaa
    10501 atgatttaaa aggtattaaa aatgtttatg ttacagaaat accaaaaggc tcaaaagtaa
    10561 atccacaagc caaaaatagt gcaatttata aagaaaaagt tgtgaaatta gaagctccta
    10621 tgagagctgg tgggtctgta acatatagca gtaatggtga tggaacaatt aatgtatata
    10681 ataatatccc ttataaatgg gaaaattcac aaaatagtga ttatagtcaa atgaataaga
    10741 taacaaaaaa agcaatagag gagaatattg aaacagtcta tattaaaccc tacgacaata
    10801 aaacagtagc taagttagca agtaaaatta gatataataa gtagttagta gattattaaa
    10861 aaggcgccta aaattaggcg tcttttttat cattagttca aaagataagc ataacgttcc
    10921 atgtaacagc atataaagta atgaatcaat tcatcactat ctaatgccac cattgttagt
    10981 tcagccttgc atggtttaac gtatctcaca taatggtaca aatcttcaaa tagtgcctgt
    11041 ttttcgggca ttttaaacac cttgtgtaca ttaaaaccta tttcttctaa tcgctttgta
    11101 atcacgccga aaacgaatgt gttcatgtga aatcactcca tcatttttaa tattcacatg
    11161 tacactaaaa aaaatccgat acatcggctt tttatttttg gtttaattta tttctatatt
    11221 gttttcacta tcgctcggac gcataagtgt taaaacacct tctaccatac ctatcacacc
    11281 cggtattccc gtccaacaaa atagtaaata aagaagagca tttttatttt tcttaacata
    11341 aaacttatga gccccgaaac ctcctagaaa aaatgctaat gcagagtaaa catatttatt
    11401 aacttgcatg atttccactc ctttaattaa ttgatttaga gaaacattaa ataatttagt
    11461 gatttctaaa attgttttta agtcgggatt acttacttct ctttcccaat tagaaataga
    11521 atgtctcgat acccctaact tttcagctaa tttttcttga gacatgtttt tactttttct
    11581 taacttttgt atttgatgtc caatttttgt ttccaaatta tttttctcct taactttaag
    11641 atatatttaa actcaatttt ttctatcgat atttatgaac attagtattt tttatgctga
    11701 aatatgtgaa caatcgcata atatcaaggt ttttctataa ttatacacgt tttaaaatac
    11761 attgtgataa tacatagaat gtaccttatc taagtatata aatgttttct ttaaaccaaa
    11821 gctaatttac cacattgggt taagtttctt attattaaag aatgaacttt gtatgatttc
    11881 aactgcttac tatcttctca tttggttatt tctctcttgt ttcttcttct ttttctgatg
    11941 attaatattg ttttgctttt cagtttcgtt cgcatagtac ataagaaagt cactagcatt
    12001 tatcgttggt aaattaatgt gattagtttg ttcattttca tgttcaatac gattgtcatg
    12061 atcatttcta tctacttgat taatatgttt ttcgataact tcttcttgat cgacatgatt
    12121 atatttctca agtttaatga gtaattgaac tttttcatta ggtgtttggt ctttgacttt
    12181 ttcaaagttt ttatcgccta tatctattct taaatcatca tcaatatgtt ctttataact
    12241 atcaatttct ttttcttttt ccttaataaa ttgcaactca acgttatatt caaagctatc
    12301 tttttctaaa tttttaagat gtttttgact aaagaaaata tctttactcg cttttttata
    12361 agtatcttct agcaatacat cattaggaat attgttattt tgtttgaaca ggtattgaac
    12421 atgagttgct tttataatat tacctttttg tgcctcatca acaataaatg ctttttcatc
    12481 atggctaaaa tgatgatgag atttagggta catttgctga acaatgagat gatttttttc
    12541 actttcagtt agataaacat ctttagctga ttgataaatc tctttacgtt tttcctctct
    12601 actttctaat ttattcttct cattgagtaa cgctttatag ttagtttcaa cagtattaac
    12661 aaactcttgt tgactatatc ctttactttc aatttctttt ctaaactgat aattttcatc
    12721 ataattttta gcatagaatt ttaattgacg tctcttaatt tcatttttaa gtaaatcatt
    12781 ttgaatttta cgaccaaagg tagacgcact gtcaaacgta tttttaattg tttctttcgc
    12841 ctctgaatag ccaacatcac gttgatatct atcaaataaa atagaataac tttgttcatt
    12901 tttacttact tgttcgttag cattttgaat aaataatcta atttcatttt gtgcttgttc
    12961 tttctcttta tcactaaata attgtgatac ttcgccacga ttatatcttc taatttgttc
    13021 atttttctgt ccgtaataag taacgggttg gtactcgata ccctcttttt cacagcgttt
    13081 tctttcattt tgttctaatc tatattcttc tcttgtaagt ggaatttcgg caattttttc
    13141 taaaccttgc gcttcaaaag aacgactatc atatcgtctt aaactttgtt cacgttctga
    13201 gtattgattt aattttaatt cccaatgctt acgaacttct ttgaaatcaa agtcggcaaa
    13261 tcgttcttgt tttctaactt tattaccatt tttatttaaa atcgcattgc cattttcatc
    13321 atattgcgtt ctcgtttttg tctttttccc ccaagtaccg tctgcattaa acggtctttg
    13381 agtaagtaga acatgggcgt gtgggttgtt tatatcatct ctatgaattg aaatatcagc
    13441 aaccattcct ttgtctacaa actcactttt tataaaatct ttagttaatt cagtttgtac
    13501 ctctctatca aaatcattag gcaaacttaa taagacttct tttgctaatc taggattttt
    13561 tgttttcgca
//
