LOCUS       NZ_CP073864            13570 bp    DNA     circular CON 17-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1276296 plasmid p1B1276296,
            complete sequence.
ACCESSION   NZ_CP073864
VERSION     NZ_CP073864.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699887
            Assembly: GCF_019329725.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 451x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019329725.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 07:01:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,669
            CDSs (total)                      :: 2,588
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 46 of 82
            Pseudo Genes (incomplete)         :: 42 of 82
            Pseudo Genes (internal stop)      :: 28 of 82
            Pseudo Genes (multiple problems)  :: 25 of 82
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073864.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1276296"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p1B1276296"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(12313..13570,1..287))
                     /gene="qac"
                     /locus_tag="KFV46_RS13175"
                     /old_locus_tag="KFV46_13185"
     CDS             complement(join(12313..13570,1..287))
                     /gene="qac"
                     /locus_tag="KFV46_RS13175"
                     /old_locus_tag="KFV46_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            466..1032
                     /gene="qacR"
                     /locus_tag="KFV46_RS13180"
                     /old_locus_tag="KFV46_13190"
     ARG             467..1029
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1283..1843
                     /locus_tag="KFV46_RS13185"
                     /old_locus_tag="KFV46_13195"
     CDS             1283..1843
                     /locus_tag="KFV46_RS13185"
                     /old_locus_tag="KFV46_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            2262..3173
                     /locus_tag="KFV46_RS13190"
                     /old_locus_tag="KFV46_13200"
     CDS             2262..3173
                     /locus_tag="KFV46_RS13190"
                     /old_locus_tag="KFV46_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(3350..3448)
                     /locus_tag="KFV46_RS13195"
                     /old_locus_tag="KFV46_13205"
     CDS             complement(3350..3448)
                     /locus_tag="KFV46_RS13195"
                     /old_locus_tag="KFV46_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            3612..4310
                     /locus_tag="KFV46_RS13200"
                     /old_locus_tag="KFV46_13210"
     CDS             3612..4310
                     /locus_tag="KFV46_RS13200"
                     /old_locus_tag="KFV46_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            4461..5165
                     /locus_tag="KFV46_RS13205"
                     /old_locus_tag="KFV46_13215"
     CDS             4461..5165
                     /locus_tag="KFV46_RS13205"
                     /old_locus_tag="KFV46_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(5214..5447)
                     /locus_tag="KFV46_RS13210"
                     /old_locus_tag="KFV46_13220"
     CDS             complement(5214..5447)
                     /locus_tag="KFV46_RS13210"
                     /old_locus_tag="KFV46_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(5524..5937)
                     /locus_tag="KFV46_RS13455"
                     /old_locus_tag="KFV46_13225"
     CDS             complement(5524..5937)
                     /locus_tag="KFV46_RS13455"
                     /old_locus_tag="KFV46_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            complement(6211..8133)
                     /locus_tag="KFV46_RS13220"
                     /old_locus_tag="KFV46_13230"
     CDS             complement(6211..8133)
                     /locus_tag="KFV46_RS13220"
                     /old_locus_tag="KFV46_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            8342..8611
                     /locus_tag="KFV46_RS13225"
                     /old_locus_tag="KFV46_13235"
     CDS             8342..8611
                     /locus_tag="KFV46_RS13225"
                     /old_locus_tag="KFV46_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            8628..8867
                     /locus_tag="KFV46_RS13230"
                     /old_locus_tag="KFV46_13240"
     CDS             8628..8867
                     /locus_tag="KFV46_RS13230"
                     /old_locus_tag="KFV46_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(9092..10027)
                     /locus_tag="KFV46_RS13235"
                     /old_locus_tag="KFV46_13245"
     CDS             complement(9092..10027)
                     /locus_tag="KFV46_RS13235"
                     /old_locus_tag="KFV46_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            10468..11229
                     /locus_tag="KFV46_RS13240"
                     /old_locus_tag="KFV46_13250"
     CDS             10468..11229
                     /locus_tag="KFV46_RS13240"
                     /old_locus_tag="KFV46_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            complement(11548..12108)
                     /locus_tag="KFV46_RS13250"
                     /old_locus_tag="KFV46_13260"
     CDS             complement(11548..12108)
                     /locus_tag="KFV46_RS13250"
                     /old_locus_tag="KFV46_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     ARG             complement(12314..13569)
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic"
                     /identity="99.76"
                     /coverage="81.32"
     ARG             complement(4..287)
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic"
                     /identity="98.95"
                     /coverage="18.48"
     Typing          complement(9093..10027)
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6281..8133)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 aggccaaata aagcaaatcc agttaataat gctttttttc ttccccattt atcagcaaag
       61 gcactcaatg gaattataaa gccagctaaa acaagagagt atatatcaac tatccataac
      121 tgttgggtac cagaaggctc taactctctt actaattccg gtaaagccat aattaatatt
      181 gtcatatcca ttgtaacaac aaacaaacta acagctaata ctactagtgc agtccatctt
      241 ttttttgatg tcatcatatc agtagttttt gtaaaaaatg aaatcattta agttccctcc
      301 aatccttata gaccgtgcga tcggtctata aggattataa tctatttttt ttacatatta
      361 caacaaaaaa tcttgcgaaa tttaaatggc tgtattgtgt tatacaatta ttctttgaat
      421 tttttgtgct atcattgata gtacttaata ctcattaaag gcgtgatgaa cttgaaagat
      481 aaaatactag gtgtcgcaaa ggaattattt ataaaaaatg gatataatgc cactactact
      541 ggagaaattg ttaaattatc agaaagtagt aaagggaatc tttattatca ctttaaaaca
      601 aaagaaaatc tatttttaga aattttaaat atagaagaat ctaaatggca agaacagtgg
      661 aaaaaggaac aaatcaaatg taaaactaat agagaaaaat tttatttata taatgaacta
      721 tctttaacaa ccgaatacta ttatccactt caaaacgcaa taattgaatt ttacactgaa
      781 tattataaaa ctaatagcat taatgaaaaa atgaataaat tagaaaacaa atatatagat
      841 gcttatcatg taatttttaa ggagggtaat ttaaatggcg aatggtgtat taatgatgtt
      901 aatgctgtta gtaaaatagc agcaaacgct gttaatggaa ttgttacctt tacacatgaa
      961 caaaatatta atgaaagaat taaacttatg aacaagttct ctcaaatttt tttaaatgga
     1021 cttagtaaat aattatatat atattcttga aataccaata aatattattt ctaaattacg
     1081 ttcgtttttg acacaaaacc tcttaactta atattaagag gtttttcttg gatatttaag
     1141 tctataaact tgtgttataa tcaatgtatc ataaatatcg aaaatcatga gtttttagat
     1201 gctgacagac gtgtttattt acatcgagaa ctatatttca ttagtaagta cccataaaac
     1261 gattaaaaaa ggtggtgttc aaatggctaa aattggttat gcacgtgtgt caacacaaga
     1321 tcaaagtctt gatggacaga ttgatacact tgaggaatat ggttgtgaac gtatctttag
     1381 cgagaaagcg agtggtcgta aaactaaaag aacggaactt gataagtgtt tagattattt
     1441 acgtgaagga gatattctag ttgtctataa attagatcgt cttggacgta caacaaaaca
     1501 attaattgag ctatctcaat ggttggatga aaatagcatt gatttacata ttatagatat
     1561 gaatgtgtca acgaaagatg cgatgggcaa gatgttcttt accatgatga gtgcattcgc
     1621 tgaacttgaa gctaatttat taagtgaacg aactaaaaaa ggtttggaag ctgcaagagc
     1681 aagagggaga aaaggtggaa gaccttcact accagatcat aagaaaagag agatcaaatt
     1741 cttatatgat gaacaaaagc tgtctggtga agaaattgct gaacaaacag gagtgagtcg
     1801 ttctactgta tataggatta ttaaagagtc aagaaaaata taaagtacta aattaaagtt
     1861 ttaatatacc ctttaattga aaaatcttct taaaaagtgt ctttaacttc agtccccttt
     1921 aacaatccac tcgcttaata gctttaaatt tctaagcatt acttgagctt tgaaaatttt
     1981 catcgattca ttaaccgaca taactggatt ttgactgtta atattcacct tgtcctctcc
     2041 aattaacaaa aaagcaaaag catataaaca acatttgttg tttatatgct tttgcttgca
     2101 ttttcttcta tagtatgcta taatgaaaag gttaagtcta tctatacatc aaaagcaata
     2161 aacttccacc tagaataaca acttattgta aatcgacaat tacaataata catcattcta
     2221 tattggaagt aaacaaaaaa cttacagaag gatgatatag agtgacacta gtatgtacca
     2281 aattcaatca tgatgctaaa tcaactgatt atttaaatat ttctattgaa gaagacactc
     2341 ttcaatatat agacccaaac cttattaaag taatatctaa tagagaagtt ggaaaagttg
     2401 cgactttggc tcaaagagca caagagcaaa ttgatgtttt ttttgaaaaa attatcgata
     2461 tacatcttag tgatttttct gatacccaaa agcgtaatga atttagaaaa ttattctctc
     2521 atttttcaga accacaccat ttacgcttag gtttttctca gaaaggtaat acaggaaaag
     2581 gtactacagc atctgaattg attaatctat ttatgaatga agatattctt tccattatct
     2641 taaatgatga cggattaaca atacctcaaa aagcaccatt aatcagaaat tttggtgatg
     2701 ataaattatc agatttaaca agtaatataa tcatgaatgt tataattgat tttaataaat
     2761 ccatgattca gtcatttcca gaaatgaaat cgtttctttc taaagaaact gtgacatatt
     2821 attatttctc tactaacggt atatgggaaa cttatgattt cactccattt ctttttgatg
     2881 gtaataaaac tttattagtt ccaaaattat tcactactta taatcagaca tctagtttag
     2941 atttgatgat aagagtatac attgaagaag aaatcattag attgcaaaaa caacgtaata
     3001 caaccaaaaa aatgacaaaa aaacaattta tcaatgaatt tataaaagga catcgtatgg
     3061 ataatatgac ctatttcttt ttaaacactt cttcaaaatc acacaaaaaa tttcaaaaag
     3121 aattagattc aaaagccaat gaaaggggta acaataacaa agatgataat tagtacttac
     3181 taatcagtat agtatagact taacatacta attattcaat tttgcattcc tccacactat
     3241 gatgttataa tttagataac aaatatgtgt tgaacatggt aaacaccgag cccgataact
     3301 attccagtag ttatcgggct ctttttggtg ttattactgt gtcgtcgctt tattgtttat
     3361 cacgattatt caaccaatgt gaaaacaacg tgattataac ccctactata ataggagcta
     3421 ctatagatac aaatatgatg ctgaacatgg tgtcacctcc ttccatgcta aaccagcaca
     3481 gaagtagaac gacgactttt atattatatc atttgtgaat tgttaagcat atgcacttcc
     3541 gataagtcat tttatgttta atgtatctat gtgataatga aaatacaaat tctattaaag
     3601 gagataattt tatgaaaaaa cgatcattac ttccaacttt attattggct agctcattaa
     3661 cattcacagg tttttcagcg acagccaatg ctgaggatgg tgccaaaacc gtatacggag
     3721 agaacttacc aaatagcgca gatacaaatg gagatggttg ggctaatgta gacttcaata
     3781 cttcatccct cccacaatct gcacaaaatc aaataagtga actttctaaa cagaaagatt
     3841 caggggaatt aactcaaatc gagtataatg agaaagtttc aagtgtattt aatgaatatc
     3901 aatcacaaag tactgatata caaaataatg agagaactca acaaggtaaa agacctttcg
     3961 gaggggtgat acctaattgt atgactcaag aagaatacca agaattagaa agtcatgttc
     4021 ctaaccctaa tagtgtatcc acagaaaaat ataatcaaat tgtaaaaaat gaaagtcaaa
     4081 agatagctaa tgaagaaaat aaaactatta atacacctca aggtgtgttg acacctgtaa
     4141 ctgaaaataa aaacaagagt tctgaaggaa tgaaaaatca agattcaaac tctcaagtat
     4201 taccagaaac aggagaaagc aaccaaagtg atatattgtc aataattggc gcttctggac
     4261 tactaatatt aggtataagt cttttaatat tcagaaaaaa ggcaatataa aaattgataa
     4321 aataaccacc gtgtttatgt aaaacatgtg gttattttat tatatattta atacatattt
     4381 ttcaactcat gtaatttacg ttttcttgct gaaaaaatat taacataatc taatttgaaa
     4441 tactgaaaag gagttgtaat atgaaaaaaa tcattttacc tatattaatt gttagtttat
     4501 cggtaatttt agtgagttgt aataataacg attcagaaca tcacaatgat aataattcta
     4561 gtgatactaa cacctcagaa aacaaagata atgaagcatc aaatgaaatt gacgaaaaaa
     4621 ctaacacatc tgatagtgcg gataattcag aaagtagtaa taaacaaaat aaagaaaaaa
     4681 atgataataa aaaacaagaa aaaaataatg atagtaataa gaagcaacaa tctactgata
     4741 aaaataaaag ttacttaaat aattttaact cgaaagaaat tgaatatgca cgagtttggt
     4801 atcagttaat atctacaaga aatgatttaa aaggtattaa aaatgtttat gttacagaaa
     4861 taccaaaagg ctcaaaagta aatccacaag ccaaaaatag tgcaatttat aaagaaaaag
     4921 ttgtgaaatt agaagctcct atgagagctg gtgggtctgt aacatatagc agtaatggtg
     4981 atggaacaat taatgtatat aataatatcc cttataaatg ggaaaattca caaaatagtg
     5041 attatagtca aatgaataag ataacaaaaa aagcaataga ggagaatatt gaaacagtct
     5101 atattaaacc ctacgacaat aaaacagtag ctaagttagc aagtaaaatt agatataata
     5161 agtagttagt agattattaa aaaggcgcct aaaattaggc gtctttttta tcattagttc
     5221 aaaagataag cataacgttc catgtaacag catataaagt aatgaatcaa ttcatcacta
     5281 tctaatgcca ccattgttag ttcagccttg catggtttaa cgtatctcac ataatggtac
     5341 aaatcttcaa atagtgcctg tttttcgggc attttaaaca ccttgtgtac attaaaacct
     5401 atttcttcta atcgctttgt aatcacgccg aaaacgaatg tgttcatgtg aaatcactcc
     5461 atcattttta atattcacat gtacactaaa aaaaatccga tacatcggct ttttattttt
     5521 ggtttaattt atttctatat tgttttcact atcgctcgga cgcataagtg ttaaaacacc
     5581 ttctaccata cctatcacac ccggtattcc cgtccaacaa aatagtaaat aaagaagagc
     5641 atttttattt ttcttaacat aaaacttatg agccccgaaa cctcctagaa aaaatgctaa
     5701 tgcagagtaa acatatttat taacttgcat gatttccact cctttaatta attgatttag
     5761 agaaacatta aataatttag tgatttctaa aattgttttt aagtcgggat tacttacttc
     5821 tctttcccaa ttagaaatag aatgtctcga tacccctaac ttttcagcta atttttcttg
     5881 agacatgttt ttactttttc ttaacttttg tatttgatgt ccaatttttg tttccaaatt
     5941 atttttctcc ttaactttaa gatatattta aactcaattt tttctatcga tatttatgaa
     6001 cattagtatt ttttatgctg aaatatgtga acaatcgcat aatatcaagg tttttctata
     6061 attatacacg ttttaaaata cattgtgata atacatagaa tgtaccttat ctaagtatat
     6121 aaatgttttc tttaaaccaa agctaattta ccacattggg ttaagtttct tattattaaa
     6181 gaatgaactt tgtatgattt caactgctta ctatcttctc atttggttat ttctctcttg
     6241 tttcttcttc tttttctgat gattaatatt gttttgcttt tcagtttcgt tcgcatagta
     6301 cataagaaag tcactagcat ttatcgttgg taaattaatg tgattagttt gttcattttc
     6361 atgttcaata cgattgtcat gatcatttct atctacttga ttaatatgtt tttcgataac
     6421 ttcttcttga tcgacatgat tatatttctc aagtttaatg agtaattgaa ctttttcatt
     6481 aggtgtttgg tctttgactt tttcaaagtt tttatcgcct atatctattc ttaaatcatc
     6541 atcaatatgt tctttataac tatcaatttc tttttctttt tccttaataa attgcaactc
     6601 aacgttatat tcaaagctat ctttttctaa atttttaaga tgtttttgac taaagaaaat
     6661 atctttactc gcttttttat aagtatcttc tagcaataca tcattaggaa tattgttatt
     6721 ttgtttgaac aggtattgaa catgagttgc ttttataata ttaccttttt gtgcctcatc
     6781 aacaataaat gctttttcat catggctaaa atgatgatga gatttagggt acatttgctg
     6841 aacaatgaga tgattttttt cactttcagt tagataaaca tctttagctg attgataaat
     6901 ctctttacgt ttttcctctc tactttctaa tttattcttc tcattgagta acgctttata
     6961 gttagtttca acagtattaa caaactcttg ttgactatat cctttacttt caatttcttt
     7021 tctaaactga taattttcat cataattttt agcatagaat tttaattgac gtctcttaat
     7081 ttcattttta agtaaatcat tttgaatttt acgaccaaag gtagacgcac tgtcaaacgt
     7141 atttttaatt gtttctttcg cctctgaata gccaacatca cgttgatatc tatcaaataa
     7201 aatagaataa ctttgttcat ttttacttac ttgttcgtta gcattttgaa taaataatct
     7261 aatttcattt tgtgcttgtt ctttctcttt atcactaaat aattgtgata cttcgccacg
     7321 attatatctt ctaatttgtt catttttctg tccgtaataa gtaacgggtt ggtactcgat
     7381 accctctttt tcacagcgtt ttctttcatt ttgttctaat ctatattctt ctcttgtaag
     7441 tggaatttcg gcaatttttt ctaaaccttg cgcttcaaaa gaacgactat catatcgtct
     7501 taaactttgt tcacgttctg agtattgatt taattttaat tcccaatgct tacgaacttc
     7561 tttgaaatca aagtcggcaa atcgttcttg ttttctaact ttattaccat ttttatttaa
     7621 aatcgcattg ccattttcat catattgcgt tctcgttttt gtctttttcc cccaagtacc
     7681 gtctgcatta aacggtcttt gagtaagtag aacatgggcg tgtgggttgt ttatatcatc
     7741 tctatgaatt gaaatatcag caaccattcc tttgtctaca aactcacttt ttataaaatc
     7801 tttagttaat tcagtttgta cctctctatc aaaatcatta ggcaaactta ataagacttc
     7861 ttttgctaat ctaggatttt ttgttttcgc attattcttt tcaactttat caacttcatt
     7921 ccataaacgc tgtctatctt tagtccattc gggtgcatgt tgaggagtta aaatcatgct
     7981 ttcaggctta atagtatgat ttctaaactt aattttttca tctgtacgtt cgctatataa
     8041 tgcttcatca gaacgataag acgctgacgc aacgatattt tttgttgctc gattaatgtt
     8101 agtcactctc aacataaagt aacctttatt catgatttta cctcctctct tttctgtttt
     8161 tttacccctg tgggaactat aatgatcaac cagtatggtt gcccattttt atgccaattt
     8221 tcaatttgca tgtaaatggg cagtgcagtg tcttaagaac tggttatatt ataccatgga
     8281 tgtgcgactt acccaatgaa ttatggtatg ctttatatag ataaactaag ggagtttttt
     8341 aatgtctaaa attaaacaaa cagatattac aaaattaagt caattagaaa aacaaattgc
     8401 acaactgaaa gagcaaaaga aaaaacaaga agaacaacta tctaataaaa ttggtggtta
     8461 ttttttaaat aaacttgatt taaatatgat caactctagt gatgaattgt ataaaattat
     8521 ggataaagtc attggagaat ttaataaaga tatcgaacaa aatagcagca acgagaacaa
     8581 ggaaaattta acagaagaca aaattaacta gaatagtaag tgatacgatg aatgatttta
     8641 aacatctcaa aaaaataaat gctgaaattg aaaaagcaga attaagaaag catagattaa
     8701 aaaatttaga tagaaaagaa agagcacata gactgattcg aaaaggtgca atgcttgaaa
     8761 agtattttga atgtgagcat ttaagtcctg atgaaacaga agaattgtta aagatgtatt
     8821 ccaactacat taatacaaat aaacccaata aatataaaaa gaagtaattt atcatgctta
     8881 caaatacttg ggtttcacta aaaagaaaaa attgctgata gtagtaacaa tgaataataa
     8941 ttgtttataa aaatgggact aaaaaagaaa agttttagtc ccacttactt aattaattac
     9001 atttcattga aaataaagtt cgcttacagt taagagaaca tctaattaaa gatattctct
     9061 aattggatca ttgttggctt attaagttgt attattgatt aaataaagaa gtgattggta
     9121 aatgatcacg tcgtttatta agcgtatgta cttctgcaaa ttcagattta agagatttca
     9181 ttaagtaccc ttgcattgct tcaacggttt catttttttg aattaatgta gctttaaaac
     9241 gcttaagaac attaacaatt tcaaattcca tatcttctaa taaataatat gcatcaatag
     9301 aattgttgaa ggatgttttg gcttttaata acacagattt aataagttta atttcagata
     9361 cagaaaagtt acttaaataa ctttgaatac tttgtggtag ttcttcaagt aattgaaatt
     9421 ttaaagcttc atcattgaat tttgaatagt agtgatttga atgatttgta tgattactag
     9481 aatatgttaa tttcctatca ttcaaatcat acatatcatt ataattagtc ttaatataat
     9541 cagtgtcatt ataatcagta tcattagact gtgaatttgg aagttcttga ccacttaaat
     9601 tcacagttct agaacttcca aattcattgt tctggaactg tgaatttgga agttccttga
     9661 tatgactggc ttcaatgtca taattaggtt ttaataggta taatctattt ggtttattaa
     9721 ggccaacacg ttcttgagtt agtaaattaa attttattaa ctcttttttt aattttataa
     9781 ttttttgttc tgcataacct aagtattcac acaaatgttt attactgaaa ataaagtaaa
     9841 tattattatt tttatctacc cattcatttt ggagggataa ttcaaaacga tcttttaata
     9901 acgcatacat aattttggct tctgcagaca ttttcttaaa atatgcatct tcaaataaat
     9961 atttaggcag ttgataaaat ctttcagttt ttacttgtga taaattatat ttttcaaaat
    10021 tgggcataat aaaaacctca tttccatttt gggattgagg taataaaaca atttttacgt
    10081 aagttatatg ttttgtgctt gcacttagat gattagatat atataataca agtatcaaaa
    10141 aaatatataa acaaacgagt gcgaatcgtt ttattaccga gagtcgatgc tacgccaata
    10201 gcatcgactc ttttaatatt tataaagtaa tattaacact tttaatggcg tatataaaga
    10261 gttttaaatg taattaaaag tacacctaaa actaccaaac gtataattcg ctattttagc
    10321 acctaattaa gtggcgaaaa tattttaagg atagataatt aggcatttag gtagttaata
    10381 gatagtaaat ttagtactaa atgatgaaat aaattttgtt aggtggtaaa atttaggtac
    10441 taaattaggt acctaagggg ggatgttatg aaaactatta aaatgattgc tgatgaatta
    10501 ggtgtaacta aacaaactat tgttaataat gctaaaagat taaatatatc ttttgaaaaa
    10561 gaaaatggaa ttaattatat taaagataat gattatttaa aaattataga aaaaatcact
    10621 aagaaagaga aaacaactca taataaagaa tcagtaaaaa atgaattatt taatgaaaat
    10681 actgcaaaag ttgaaaagta tatacataat aattcaaata gttctgagac attaaaaaca
    10741 aaagtaaacg aattagaaaa gcaaatagaa atctttgaaa ctagagctaa aaatgatgaa
    10801 aaatatattg agaatctaac taaacaatta gatcaacaaa atagcaatgt taatacatta
    10861 aataaactgt tggagaatca acaaatatta tctttagaaa acaataaaaa aattcaaaaa
    10921 ctagaaagcc aattagaaga agaaagacag cttaaatatt cttttgatac aacgatgaat
    10981 aatagacaga atattaatcc acaagaagca acttttactg aagaatctca aaatatcaac
    11041 caacaacaaa aagaatgtca accaaccgaa gtaaatcata aaaatataac tgaagagaat
    11101 aagcatgaca gtacagatga attaacaaaa gaggataagt ttgttgaaaa aacgaagaac
    11161 gattccagtg ataataggga ggtagaacca cctaaaaaga gattttggag tcgtttattt
    11221 ggtaactaat tgcaattaaa aaggcacaca agttcatgaa cttgttgtgc ctttttaatg
    11281 tgaatcaaac ttctgataat catcagttat gttaaactct aacaaaaact aaattatcag
    11341 aagaattatc tagggtatta aacataggta aatcaatttt ctatcgccaa tagtataaac
    11401 atcatccata ttaatctgaa gatagctaca taattcttta acagcatagc ctttatttgt
    11461 ataaggcgcc ataaactcta ttaaatatgg tttgttttga ggtttctatg tttaatcatt
    11521 taatttttca ttatataaat cgaataatta taaaaaatca tcatactttt tattttttaa
    11581 aaatatttga aaatttaata gtaatccaat tataagatca aatatcatta tcattataat
    11641 caaggcaact ataatcactg tgacactcgt tacataacaa gctagtaatc tccagataaa
    11701 attgacattt tcaccaattc cataacttaa catattacaa agtagtaaaa gaataataat
    11761 acctatactg taaattaaaa ttgtcagtgt atatttttta ataactctat taggaaacaa
    11821 tagttcatct attaggttct ttttcaccag tatctctcgt tcaattctat ctttgtcaca
    11881 gattttgatt ttactatttt ttattttttc ctctaataaa ataacctcga acgatctgct
    11941 aatttttaat aaatatgcag gaaaagctaa tgttaagcat aatgtaatta attcatttac
    12001 attgttcatg taacttataa aatagatgaa agttgctact agtgctccta aaaatatagt
    12061 tatattaagt tctacatcaa atattttaaa aatatctgct ctattcatca gttaatcatc
    12121 ctttcaaggt ttattaatac taataaatta ttagatatag gtatatcata ttattaattt
    12181 aagaaattgt cttttataat tttacttaat aataaaaaag tagaaccatt taaaattaat
    12241 ggttctactt tttaactatt actaatttta aaaataaact aatctacaat atctaaaaat
    12301 atatgtttag tactattttt gtttagttat atcaagtgat ttgggaatta acaaatagac
    12361 aactattgaa atgataatca tgataatccc acctactaaa gcagttgcta caaaagcatc
    12421 attaaatgat gttacagcct cgtttgcaag ctgttttatc cccgtagctt tagctacttc
    12481 gacagcgccc actacagatt cttcagctac atgagctaaa tctccaacta taccttttga
    12541 tgaaaaagat gaaatatcta aaaatacacg ataaagcata gaagataggc taccaagtac
    12601 tgctacacca aaaacatttc caaggtcata catagactct tcaacagcag ctgcattacc
    12661 tgcttttgat gtaggtgttt ctaacattat tagagcagat gcaactgcta gtgaagccat
    12721 accagctcca actaaaatta atgctaaagc cattgtagaa tatgataatg gatgaccaaa
    12781 gaaatacata ataaacatgc caatggctgc aattccaatt ccggaaggta acactatttt
    12841 cggtccaaat cgcgccgcta atccgggtgc aattggtgca aacaccatat ctcctattgc
    12901 cataggtaat aggtataagc cagctttaaa aggagaaagt tcttccacaa cctgtaacca
    12961 ttgtgaagct aataacaaaa cagatgccat tgcaaaaatt gtcataaatg cagcaattgt
    13021 accagctgaa aatgatctct ttttaaaaag tcttacgtct aacattggat cagaacttga
    13081 taaattacgt ttaacaaata tcactatcat ggtaattgct aatactatta caacccatgg
    13141 aataatatct gctagtcctt cttttgaaaa ttctttgata ctccatacca gtccaatcat
    13201 gcctgcaatt gataaaattg tagaaggaat atcccacgag tgagactttt cttttgataa
    13261 cttagactct ggtaataaaa ataaacctgc tacaactgct attatcgcaa acggtacatt
    13321 aattaaaaat gccgagtgcc atgaaaattg ctcaagtaaa gctcctccga taattggtcc
    13381 aaaaacagca cctatcgatg aagcgattga ccatacagct aatgcagtgg ccctttcttt
    13441 agggttttca aaaattactc ttatcattga aagagtagtt ggcattatta aagcacctgc
    13501 aataccaagt aaaaatcgaa tagctattac gaactctgca ctttctgcga aaaatatagc
    13561 taatgaaacg
//
