LOCUS       NZ_CP073861            13570 bp    DNA     circular CON 16-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1276219 plasmid p1B1276219,
            complete sequence.
ACCESSION   NZ_CP073861
VERSION     NZ_CP073861.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699886
            Assembly: GCF_019329705.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 440x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019329705.1-RS_2024_04_16
            Annotation Date                   :: 04/16/2024 07:53:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,675
            CDSs (total)                      :: 2,594
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 41 of 79
            Pseudo Genes (incomplete)         :: 43 of 79
            Pseudo Genes (internal stop)      :: 28 of 79
            Pseudo Genes (multiple problems)  :: 24 of 79
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073861.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1276219"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p1B1276219"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(11867..13570,1..219))
                     /locus_tag="KFV45_RS00005"
                     /old_locus_tag="KFV45_00005"
     CDS             complement(join(11867..13570,1..219))
                     /locus_tag="KFV45_RS00005"
                     /old_locus_tag="KFV45_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            428..697
                     /locus_tag="KFV45_RS00010"
                     /old_locus_tag="KFV45_00010"
     CDS             428..697
                     /locus_tag="KFV45_RS00010"
                     /old_locus_tag="KFV45_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            714..953
                     /locus_tag="KFV45_RS00015"
                     /old_locus_tag="KFV45_00015"
     CDS             714..953
                     /locus_tag="KFV45_RS00015"
                     /old_locus_tag="KFV45_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(1178..2113)
                     /locus_tag="KFV45_RS00020"
                     /old_locus_tag="KFV45_00020"
     CDS             complement(1178..2113)
                     /locus_tag="KFV45_RS00020"
                     /old_locus_tag="KFV45_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            2554..3315
                     /locus_tag="KFV45_RS00025"
                     /old_locus_tag="KFV45_00025"
     CDS             2554..3315
                     /locus_tag="KFV45_RS00025"
                     /old_locus_tag="KFV45_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            complement(3634..4194)
                     /locus_tag="KFV45_RS00035"
                     /old_locus_tag="KFV45_00035"
     CDS             complement(3634..4194)
                     /locus_tag="KFV45_RS00035"
                     /old_locus_tag="KFV45_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(4399..5943)
                     /gene="qac"
                     /locus_tag="KFV45_RS00040"
                     /old_locus_tag="KFV45_00040"
     CDS             complement(4399..5943)
                     /gene="qac"
                     /locus_tag="KFV45_RS00040"
                     /old_locus_tag="KFV45_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            6122..6688
                     /gene="qacR"
                     /locus_tag="KFV45_RS00045"
                     /old_locus_tag="KFV45_00045"
     ARG             6123..6685
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6939..7499
                     /locus_tag="KFV45_RS00050"
                     /old_locus_tag="KFV45_00050"
     CDS             6939..7499
                     /locus_tag="KFV45_RS00050"
                     /old_locus_tag="KFV45_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            7918..8829
                     /locus_tag="KFV45_RS00055"
                     /old_locus_tag="KFV45_00055"
     CDS             7918..8829
                     /locus_tag="KFV45_RS00055"
                     /old_locus_tag="KFV45_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(9006..9104)
                     /locus_tag="KFV45_RS00060"
                     /old_locus_tag="KFV45_00060"
     CDS             complement(9006..9104)
                     /locus_tag="KFV45_RS00060"
                     /old_locus_tag="KFV45_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            9268..9966
                     /locus_tag="KFV45_RS00065"
                     /old_locus_tag="KFV45_00065"
     CDS             9268..9966
                     /locus_tag="KFV45_RS00065"
                     /old_locus_tag="KFV45_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            10117..10821
                     /locus_tag="KFV45_RS00070"
                     /old_locus_tag="KFV45_00070"
     CDS             10117..10821
                     /locus_tag="KFV45_RS00070"
                     /old_locus_tag="KFV45_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(10870..11103)
                     /locus_tag="KFV45_RS00075"
                     /old_locus_tag="KFV45_00075"
     CDS             complement(10870..11103)
                     /locus_tag="KFV45_RS00075"
                     /old_locus_tag="KFV45_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(11180..11593)
                     /locus_tag="KFV45_RS13455"
                     /old_locus_tag="KFV45_00080"
     CDS             complement(11180..11593)
                     /locus_tag="KFV45_RS13455"
                     /old_locus_tag="KFV45_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     ARG             complement(4403..5943)
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          complement(1179..2113)
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11937..13570)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 ttcattccat aaacgctgtc tatctttagt ccattcgggt gcatgttgag gagttaaaat
       61 catgctttca ggcttaatag tatgatttct aaacttaatt ttttcatctg tacgttcgct
      121 atataatgct tcatcagaac gataagacgc tgacgcaacg atattttttg ttgctcgatt
      181 aatgttagtc actctcaaca taaagtaacc tttattcatg attttacctc ctctcttttc
      241 tgttttttta cccctgtggg aactataatg atcaaccagt atggttgccc atttttatgc
      301 caattttcaa tttgcatgta aatgggcagt gcagtgtctt aagaactggt tatattatac
      361 catggatgtg cgacttaccc aatgaattat ggtatgcttt atatagataa actaagggag
      421 ttttttaatg tctaaaatta aacaaacaga tattacaaaa ttaagtcaat tagaaaaaca
      481 aattgcacaa ctgaaagagc aaaagaaaaa acaagaagaa caactatcta ataaaattgg
      541 tggttatttt ttaaataaac ttgatttaaa tatgatcaac tctagtgatg aattgtataa
      601 aattatggat aaagtcattg gagaatttaa taaagatatc gaacaaaata gcagcaacga
      661 gaacaaggaa aatttaacag aagacaaaat taactagaat agtaagtgat acgatgaatg
      721 attttaaaca tctcaaaaaa ataaatgctg aaattgaaaa agcagaatta agaaagcata
      781 gattaaaaaa tttagataga aaagaaagag cacatagact gattcgaaaa ggtgcaatgc
      841 ttgaaaagta ttttgaatgt gagcatttaa gtcctgatga aacagaagaa ttgttaaaga
      901 tgtattccaa ctacattaat acaaataaac ccaataaata taaaaagaag taatttatca
      961 tgcttacaaa tacttgggtt tcactaaaaa gaaaaaattg ctgatagtag taacaatgaa
     1021 taataattgt ttataaaaat gggactaaaa aagaaaagtt ttagtcccac ttacttaatt
     1081 aattacattt cattgaaaat aaagttcgct tacagttaag agaacatcta attaaagata
     1141 ttctctaatt ggatcattgt tggcttatta agttgtatta ttgattaaat aaagaagtga
     1201 ttggtaaatg atcacgtcgt ttattaagcg tatgtacttc tgcaaattca gatttaagag
     1261 atttcattaa gtacccttgc attgcttcaa cggtttcatt tttttgaatt aatgtagctt
     1321 taaaacgctt aagaacatta acaatttcaa attccatatc ttctaataaa taatatgcat
     1381 caatagaatt gttgaaggat gttttggctt ttaataacac agatttaata agtttaattt
     1441 cagatacaga aaagttactt aaataacttt gaatactttg tggtagttct tcaagtaatt
     1501 gaaattttaa agcttcatca ttgaattttg aatagtagtg atttgaatga tttgtatgat
     1561 tactagaata tgttaatttc ctatcattca aatcatacat atcattataa ttagtcttaa
     1621 tataatcagt gtcattataa tcagtatcat tagactgtga atttggaagt tcttgaccac
     1681 ttaaattcac agttctagaa cttccaaatt cattgttctg gaactgtgaa tttggaagtt
     1741 ccttgatatg actggcttca atgtcataat taggttttaa taggtataat ctatttggtt
     1801 tattaaggcc aacacgttct tgagttagta aattaaattt tattaactct ttttttaatt
     1861 ttataatttt ttgttctgca taacctaagt attcacacaa atgtttatta ctgaaaataa
     1921 agtaaatatt attattttta tctacccatt cattttggag ggataattca aaacgatctt
     1981 ttaataacgc atacataatt ttggcttctg cagacatttt cttaaaatat gcatcttcaa
     2041 ataaatattt aggcagttga taaaatcttt cagtttttac ttgtgataaa ttatattttt
     2101 caaaattggg cataataaaa acctcatttc cattttggga ttgaggtaat aaaacaattt
     2161 ttacgtaagt tatatgtttt gtgcttgcac ttagatgatt agatatatat aatacaagta
     2221 tcaaaaaaat atataaacaa acgagtgcga atcgttttat taccgagagt cgatgctacg
     2281 ccaatagcat cgactctttt aatatttata aagtaatatt aacactttta atggcgtata
     2341 taaagagttt taaatgtaat taaaagtaca cctaaaacta ccaaacgtat aattcgctat
     2401 tttagcacct aattaagtgg cgaaaatatt ttaaggatag ataattaggc atttaggtag
     2461 ttaatagata gtaaatttag tactaaatga tgaaataaat tttgttaggt ggtaaaattt
     2521 aggtactaaa ttaggtacct aaggggggat gttatgaaaa ctattaaaat gattgctgat
     2581 gaattaggtg taactaaaca aactattgtt aataatgcta aaagattaaa tatatctttt
     2641 gaaaaagaaa atggaattaa ttatattaaa gataatgatt atttaaaaat tatagaaaaa
     2701 atcactaaga aagagaaaac aactcataat aaagaatcag taaaaaatga attatttaat
     2761 gaaaatactg caaaagttga aaagtatata cataataatt caaatagttc tgagacatta
     2821 aaaacaaaag taaacgaatt agaaaagcaa atagaaatct ttgaaactag agctaaaaat
     2881 gatgaaaaat atattgagaa tctaactaaa caattagatc aacaaaatag caatgttaat
     2941 acattaaata aactgttgga gaatcaacaa atattatctt tagaaaacaa taaaaaaatt
     3001 caaaaactag aaagccaatt agaagaagaa agacagctta aatattcttt tgatacaacg
     3061 atgaataata gacagaatat taatccacaa gaagcaactt ttactgaaga atctcaaaat
     3121 atcaaccaac aacaaaaaga atgtcaacca accgaagtaa atcataaaaa tataactgaa
     3181 gagaataagc atgacagtac agatgaatta acaaaagagg ataagtttgt tgaaaaaacg
     3241 aagaacgatt ccagtgataa tagggaggta gaaccaccta aaaagagatt ttggagtcgt
     3301 ttatttggta actaattgca attaaaaagg cacacaagtt catgaacttg ttgtgccttt
     3361 ttaatgtgaa tcaaacttct gataatcatc agttatgtta aactctaaca aaaactaaat
     3421 tatcagaaga attatctagg gtattaaaca taggtaaatc aattttctat cgccaatagt
     3481 ataaacatca tccatattaa tctgaagata gctacataat tctttaacag catagccttt
     3541 atttgtataa ggcgccataa actctattaa atatggtttg ttttgaggtt tctatgttta
     3601 atcatttaat ttttcattat ataaatcgaa taattataaa aaatcatcat actttttatt
     3661 ttttaaaaat atttgaaaat ttaatagtaa tccaattata agatcaaata tcattatcat
     3721 tataatcaag gcaactataa tcactgtgac actcgttaca taacaagcta gtaatctcca
     3781 gataaaattg acattttcac caattccata acttaacata ttacaaagta gtaaaagaat
     3841 aataatacct atactgtaaa ttaaaattgt cagtgtatat tttttaataa ctctattagg
     3901 aaacaatagt tcatctatta ggttcttttt caccagtatc tctcgttcaa ttctatcttt
     3961 gtcacagatt ttgattttac tattttttat tttttcctct aataaaataa cctcgaacga
     4021 tctgctaatt tttaataaat atgcaggaaa agctaatgtt aagcataatg taattaattc
     4081 atttacattg ttcatgtaac ttataaaata gatgaaagtt gctactagtg ctcctaaaaa
     4141 tatagttata ttaagttcta catcaaatat tttaaaaata tctgctctat tcatcagtta
     4201 atcatccttt caaggtttat taatactaat aaattattag atataggtat atcatattat
     4261 taatttaaga aattgtcttt tataatttta cttaataata aaaaagtaga accatttaaa
     4321 attaatggtt ctacttttta actattacta attttaaaaa taaactaatc tacaatatct
     4381 aaaaatatat gtttagtact atttttgttt agttatatca agtgatttgg gaattaacaa
     4441 atagacaact attgaaatga taatcatgat aatcccacct actaaagcag ttgctacaaa
     4501 agcatcatta aatgatgtta cagcctcgtt tgcaagctgt tttatccccg tagctttagc
     4561 tacttcgaca gcgcccacta cagattcttc agctacatga gctaaatctc caactatacc
     4621 ttttgatgaa aaagatgaaa tatctaaaaa tacacgataa agcatagaag ataggctacc
     4681 aagtactgct acaccaaaaa catttccaag gtcatacata gactcttcaa cagcagctgc
     4741 attacctgct tttgatgtag gtgtttctaa cattattaga gcagatgcaa ctgctagtga
     4801 agccatacca gctccaacta aaattaatgc taaagccatt gtagaatatg ataatggatg
     4861 accaaagaaa tacataataa acatgccaat ggctgcaatt ccaattccgg aaggtaacac
     4921 tattttcggt ccaaatcgcg ccgctaatcc gggtgcaatt ggtgcaaaca ccatatctcc
     4981 tattgccata ggtaataggt ataagccagc tttaaaagga gaaagttctt ccacaacctg
     5041 taaccattgt gaagctaata acaaaacaga tgccattgca aaaattgtca taaatgcagc
     5101 aattgtacca gctgaaaatg atctcttttt aaaaagtctt acgtctaaca ttggatcaga
     5161 acttgataaa ttacgtttaa caaatatcac tatcatggta attgctaata ctattacaac
     5221 ccatggaata atatctgcta gtccttcttt tgaaaattct ttgatactcc ataccagtcc
     5281 aatcatgcct gcaattgata aaattgtaga aggaatatcc cacgagtgag acttttcttt
     5341 tgataactta gactctggta ataaaaataa acctgctaca actgctatta tcgcaaacgg
     5401 tacattaatt aaaaatgccg agtgccatga aaattgctca agtaaagctc ctccgataat
     5461 tggtccaaaa acagcaccta tcgatgaagc gattgaccat acagctaatg cagtggccct
     5521 ttctttaggg ttttcaaaaa ttactcttat cattgaaaga gtagttggca ttattaaagc
     5581 acctgcaata ccaagtaaaa atcgaatagc tattacgaac tctgcacttt ctgcgaaaaa
     5641 tatagctaat gaaacgaggc caaataaagc aaatccagtt aataatgctt tttttcttcc
     5701 ccatttatca gcaaaggcac tcaatggaat tataaagcca gctaaaacaa gagagtatat
     5761 atcaactatc cataactgtt gggtaccaga aggctctaac tctcttacta attccggtaa
     5821 agccataatt aatattgtca tatccattgt aacaacaaac aaactaacag ctaatactac
     5881 tagtgcagtc catctttttt ttgatgtcat catatcagta gtttttgtaa aaaatgaaat
     5941 catttaagtt ccctccaatc cttatagacc gtgcgatcgg tctataagga ttataatcta
     6001 ttttttttac atattacaac aaaaaatctt gcgaaattta aatggctgta ttgtgttata
     6061 caattattct ttgaattttt tgtgctatca ttgatagtac ttaatactca ttaaaggcgt
     6121 gatgaacttg aaagataaaa tactaggtgt cgcaaaggaa ttatttataa aaaatggata
     6181 taatgccact actactggag aaattgttaa attatcagaa agtagtaaag ggaatcttta
     6241 ttatcacttt aaaacaaaag aaaatctatt tttagaaatt ttaaatatag aagaatctaa
     6301 atggcaagaa cagtggaaaa aggaacaaat caaatgtaaa actaatagag aaaaatttta
     6361 tttatataat gaactatctt taacaaccga atactattat ccacttcaaa acgcaataat
     6421 tgaattttac actgaatatt ataaaactaa tagcattaat gaaaaaatga ataaattaga
     6481 aaacaaatat atagatgctt atcatgtaat ttttaaggag ggtaatttaa atggcgaatg
     6541 gtgtattaat gatgttaatg ctgttagtaa aatagcagca aacgctgtta atggaattgt
     6601 tacctttaca catgaacaaa atattaatga aagaattaaa cttatgaaca agttctctca
     6661 aattttttta aatggactta gtaaataatt atatatatat tcttgaaata ccaataaata
     6721 ttatttctaa attacgttcg tttttgacac aaaacctctt aacttaatat taagaggttt
     6781 ttcttggata tttaagtcta taaacttgtg ttataatcaa tgtatcataa atatcgaaaa
     6841 tcatgagttt ttagatgctg acagacgtgt ttatttacat cgagaactat atttcattag
     6901 taagtaccca taaaacgatt aaaaaaggtg gtgttcaaat ggctaaaatt ggttatgcac
     6961 gtgtgtcaac acaagatcaa agtcttgatg gacagattga tacacttgag gaatatggtt
     7021 gtgaacgtat ctttagcgag aaagcgagtg gtcgtaaaac taaaagaacg gaacttgata
     7081 agtgtttaga ttatttacgt gaaggagata ttctagttgt ctataaatta gatcgtcttg
     7141 gacgtacaac aaaacaatta attgagctat ctcaatggtt ggatgaaaat agcattgatt
     7201 tacatattat agatatgaat gtgtcaacga aagatgcgat gggcaagatg ttctttacca
     7261 tgatgagtgc attcgctgaa cttgaagcta atttattaag tgaacgaact aaaaaaggtt
     7321 tggaagctgc aagagcaaga gggagaaaag gtggaagacc ttcactacca gatcataaga
     7381 aaagagagat caaattctta tatgatgaac aaaagctgtc tggtgaagaa attgctgaac
     7441 aaacaggagt gagtcgttct actgtatata ggattattaa agagtcaaga aaaatataaa
     7501 gtactaaatt aaagttttaa tatacccttt aattgaaaaa tcttcttaaa aagtgtcttt
     7561 aacttcagtc ccctttaaca atccactcgc ttaatagctt taaatttcta agcattactt
     7621 gagctttgaa aattttcatc gattcattaa ccgacataac tggattttga ctgttaatat
     7681 tcaccttgtc ctctccaatt aacaaaaaag caaaagcata taaacaacat ttgttgttta
     7741 tatgcttttg cttgcatttt cttctatagt atgctataat gaaaaggtta agtctatcta
     7801 tacatcaaaa gcaataaact tccacctaga ataacaactt attgtaaatc gacaattaca
     7861 ataatacatc attctatatt ggaagtaaac aaaaaactta cagaaggatg atatagagtg
     7921 acactagtat gtaccaaatt caatcatgat gctaaatcaa ctgattattt aaatatttct
     7981 attgaagaag acactcttca atatatagac ccaaacctta ttaaagtaat atctaataga
     8041 gaagttggaa aagttgcgac tttggctcaa agagcacaag agcaaattga tgtttttttt
     8101 gaaaaaatta tcgatataca tcttagtgat ttttctgata cccaaaagcg taatgaattt
     8161 agaaaattat tctctcattt ttcagaacca caccatttac gcttaggttt ttctcagaaa
     8221 ggtaatacag gaaaaggtac tacagcatct gaattgatta atctatttat gaatgaagat
     8281 attctttcca ttatcttaaa tgatgacgga ttaacaatac ctcaaaaagc accattaatc
     8341 agaaattttg gtgatgataa attatcagat ttaacaagta atataatcat gaatgttata
     8401 attgatttta ataaatccat gattcagtca tttccagaaa tgaaatcgtt tctttctaaa
     8461 gaaactgtga catattatta tttctctact aacggtatat gggaaactta tgatttcact
     8521 ccatttcttt ttgatggtaa taaaacttta ttagttccaa aattattcac tacttataat
     8581 cagacatcta gtttagattt gatgataaga gtatacattg aagaagaaat cattagattg
     8641 caaaaacaac gtaatacaac caaaaaaatg acaaaaaaac aatttatcaa tgaatttata
     8701 aaaggacatc gtatggataa tatgacctat ttctttttaa acacttcttc aaaatcacac
     8761 aaaaaatttc aaaaagaatt agattcaaaa gccaatgaaa ggggtaacaa taacaaagat
     8821 gataattagt acttactaat cagtatagta tagacttaac atactaatta ttcaattttg
     8881 cattcctcca cactatgatg ttataattta gataacaaat atgtgttgaa catggtaaac
     8941 accgagcccg ataactattc cagtagttat cgggctcttt ttggtgttat tactgtgtcg
     9001 tcgctttatt gtttatcacg attattcaac caatgtgaaa acaacgtgat tataacccct
     9061 actataatag gagctactat agatacaaat atgatgctga acatggtgtc acctccttcc
     9121 atgctaaacc agcacagaag tagaacgacg acttttatat tatatcattt gtgaattgtt
     9181 aagcatatgc acttccgata agtcatttta tgtttaatgt atctatgtga taatgaaaat
     9241 acaaattcta ttaaaggaga taattttatg aaaaaacgat cattacttcc aactttatta
     9301 ttggctagct cattaacatt cacaggtttt tcagcgacag ccaatgctga ggatggtgcc
     9361 aaaaccgtat acggagagaa cttaccaaat agcgcagata caaatggaga tggttgggct
     9421 aatgtagact tcaatacttc atccctccca caatctgcac aaaatcaaat aagtgaactt
     9481 tctaaacaga aagattcagg ggaattaact caaatcgagt ataatgagaa agtttcaagt
     9541 gtatttaatg aatatcaatc acaaagtact gatatacaaa ataatgagag aactcaacaa
     9601 ggtaaaagac ctttcggagg ggtgatacct aattgtatga ctcaagaaga ataccaagaa
     9661 ttagaaagtc atgttcctaa ccctaatagt gtatccacag aaaaatataa tcaaattgta
     9721 aaaaatgaaa gtcaaaagat agctaatgaa gaaaataaaa ctattaatac acctcaaggt
     9781 gtgttgacac ctgtaactga aaataaaaac aagagttctg aaggaatgaa aaatcaagat
     9841 tcaaactctc aagtattacc agaaacagga gaaagcaacc aaagtgatat attgtcaata
     9901 attggcgctt ctggactact aatattaggt ataagtcttt taatattcag aaaaaaggca
     9961 atataaaaat tgataaaata accaccgtgt ttatgtaaaa catgtggtta ttttattata
    10021 tatttaatac atatttttca actcatgtaa tttacgtttt cttgctgaaa aaatattaac
    10081 ataatctaat ttgaaatact gaaaaggagt tgtaatatga aaaaaatcat tttacctata
    10141 ttaattgtta gtttatcggt aattttagtg agttgtaata ataacgattc agaacatcac
    10201 aatgataata attctagtga tactaacacc tcagaaaaca aagataatga agcatcaaat
    10261 gaaattgacg aaaaaactaa cacatctgat agtgcggata attcagaaag tagtaataaa
    10321 caaaataaag aaaaaaatga taataaaaaa caagaaaaaa ataatgatag taataagaag
    10381 caacaatcta ctgataaaaa taaaagttac ttaaataatt ttaactcgaa agaaattgaa
    10441 tatgcacgag tttggtatca gttaatatct acaagaaatg atttaaaagg tattaaaaat
    10501 gtttatgtta cagaaatacc aaaaggctca aaagtaaatc cacaagccaa aaatagtgca
    10561 atttataaag aaaaagttgt gaaattagaa gctcctatga gagctggtgg gtctgtaaca
    10621 tatagcagta atggtgatgg aacaattaat gtatataata atatccctta taaatgggaa
    10681 aattcacaaa atagtgatta tagtcaaatg aataagataa caaaaaaagc aatagaggag
    10741 aatattgaaa cagtctatat taaaccctac gacaataaaa cagtagctaa gttagcaagt
    10801 aaaattagat ataataagta gttagtagat tattaaaaag gcgcctaaaa ttaggcgtct
    10861 tttttatcat tagttcaaaa gataagcata acgttccatg taacagcata taaagtaatg
    10921 aatcaattca tcactatcta atgccaccat tgttagttca gccttgcatg gtttaacgta
    10981 tctcacataa tggtacaaat cttcaaatag tgcctgtttt tcgggcattt taaacacctt
    11041 gtgtacatta aaacctattt cttctaatcg ctttgtaatc acgccgaaaa cgaatgtgtt
    11101 catgtgaaat cactccatca tttttaatat tcacatgtac actaaaaaaa atccgataca
    11161 tcggcttttt atttttggtt taatttattt ctatattgtt ttcactatcg ctcggacgca
    11221 taagtgttaa aacaccttct accataccta tcacacccgg tattcccgtc caacaaaata
    11281 gtaaataaag aagagcattt ttatttttct taacataaaa cttatgagcc ccgaaacctc
    11341 ctagaaaaaa tgctaatgca gagtaaacat atttattaac ttgcatgatt tccactcctt
    11401 taattaattg atttagagaa acattaaata atttagtgat ttctaaaatt gtttttaagt
    11461 cgggattact tacttctctt tcccaattag aaatagaatg tctcgatacc cctaactttt
    11521 cagctaattt ttcttgagac atgtttttac tttttcttaa cttttgtatt tgatgtccaa
    11581 tttttgtttc caaattattt ttctccttaa ctttaagata tatttaaact caattttttc
    11641 tatcgatatt tatgaacatt agtatttttt atgctgaaat atgtgaacaa tcgcataata
    11701 tcaaggtttt tctataatta tacacgtttt aaaatacatt gtgataatac atagaatgta
    11761 ccttatctaa gtatataaat gttttcttta aaccaaagct aatttaccac attgggttaa
    11821 gtttcttatt attaaagaat gaactttgta tgatttcaac tgcttactat cttctcattt
    11881 ggttatttct ctcttgtttc ttcttctttt tctgatgatt aatattgttt tgcttttcag
    11941 tttcgttcgc atagtacata agaaagtcac tagcatttat cgttggtaaa ttaatgtgat
    12001 tagtttgttc attttcatgt tcaatacgat tgtcatgatc atttctatct acttgattaa
    12061 tatgtttttc gataacttct tcttgatcga catgattata tttctcaagt ttaatgagta
    12121 attgaacttt ttcattaggt gtttggtctt tgactttttc aaagttttta tcgcctatat
    12181 ctattcttaa atcatcatca atatgttctt tataactatc aatttctttt tctttttcct
    12241 taataaattg caactcaacg ttatattcaa agctatcttt ttctaaattt ttaagatgtt
    12301 tttgactaaa gaaaatatct ttactcgctt ttttataagt atcttctagc aatacatcat
    12361 taggaatatt gttattttgt ttgaacaggt attgaacatg agttgctttt ataatattac
    12421 ctttttgtgc ctcatcaaca ataaatgctt tttcatcatg gctaaaatga tgatgagatt
    12481 tagggtacat ttgctgaaca atgagatgat ttttttcact ttcagttaga taaacatctt
    12541 tagctgattg ataaatctct ttacgttttt cctctctact ttctaattta ttcttctcat
    12601 tgagtaacgc tttatagtta gtttcaacag tattaacaaa ctcttgttga ctatatcctt
    12661 tactttcaat ttcttttcta aactgataat tttcatcata atttttagca tagaatttta
    12721 attgacgtct cttaatttca tttttaagta aatcattttg aattttacga ccaaaggtag
    12781 acgcactgtc aaacgtattt ttaattgttt ctttcgcctc tgaatagcca acatcacgtt
    12841 gatatctatc aaataaaata gaataacttt gttcattttt acttacttgt tcgttagcat
    12901 tttgaataaa taatctaatt tcattttgtg cttgttcttt ctctttatca ctaaataatt
    12961 gtgatacttc gccacgatta tatcttctaa tttgttcatt tttctgtccg taataagtaa
    13021 cgggttggta ctcgataccc tctttttcac agcgttttct ttcattttgt tctaatctat
    13081 attcttctct tgtaagtgga atttcggcaa ttttttctaa accttgcgct tcaaaagaac
    13141 gactatcata tcgtcttaaa ctttgttcac gttctgagta ttgatttaat tttaattccc
    13201 aatgcttacg aacttctttg aaatcaaagt cggcaaatcg ttcttgtttt ctaactttat
    13261 taccattttt atttaaaatc gcattgccat tttcatcata ttgcgttctc gtttttgtct
    13321 ttttccccca agtaccgtct gcattaaacg gtctttgagt aagtagaaca tgggcgtgtg
    13381 ggttgtttat atcatctcta tgaattgaaa tatcagcaac cattcctttg tctacaaact
    13441 cactttttat aaaatcttta gttaattcag tttgtacctc tctatcaaaa tcattaggca
    13501 aacttaataa gacttctttt gctaatctag gattttttgt tttcgcatta ttcttttcaa
    13561 ctttatcaac
//
