LOCUS       NZ_CP073860            13570 bp    DNA     circular CON 16-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1275857 plasmid p1B1275857,
            complete sequence.
ACCESSION   NZ_CP073860
VERSION     NZ_CP073860.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699885
            Assembly: GCF_019329685.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 317x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019329685.1-RS_2024_04_16
            Annotation Date                   :: 04/16/2024 08:00:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,680
            CDSs (total)                      :: 2,599
            Genes (coding)                    :: 2,522
            CDSs (with protein)               :: 2,522
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 41 of 77
            Pseudo Genes (incomplete)         :: 42 of 77
            Pseudo Genes (internal stop)      :: 27 of 77
            Pseudo Genes (multiple problems)  :: 24 of 77
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073860.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1275857"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p1B1275857"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            join(13403..13570,1..1755)
                     /locus_tag="KFV44_RS13215"
                     /old_locus_tag="KFV44_13225"
     CDS             join(13403..13570,1..1755)
                     /locus_tag="KFV44_RS13215"
                     /old_locus_tag="KFV44_13225"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            2029..2442
                     /locus_tag="KFV44_RS13510"
                     /old_locus_tag="KFV44_13230"
     CDS             2029..2442
                     /locus_tag="KFV44_RS13510"
                     /old_locus_tag="KFV44_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            2519..2752
                     /locus_tag="KFV44_RS13225"
                     /old_locus_tag="KFV44_13235"
     CDS             2519..2752
                     /locus_tag="KFV44_RS13225"
                     /old_locus_tag="KFV44_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(2801..3505)
                     /locus_tag="KFV44_RS13230"
                     /old_locus_tag="KFV44_13240"
     CDS             complement(2801..3505)
                     /locus_tag="KFV44_RS13230"
                     /old_locus_tag="KFV44_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(3656..4354)
                     /locus_tag="KFV44_RS13235"
                     /old_locus_tag="KFV44_13245"
     CDS             complement(3656..4354)
                     /locus_tag="KFV44_RS13235"
                     /old_locus_tag="KFV44_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            4518..4616
                     /locus_tag="KFV44_RS13240"
                     /old_locus_tag="KFV44_13250"
     CDS             4518..4616
                     /locus_tag="KFV44_RS13240"
                     /old_locus_tag="KFV44_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(4793..5704)
                     /locus_tag="KFV44_RS13245"
                     /old_locus_tag="KFV44_13255"
     CDS             complement(4793..5704)
                     /locus_tag="KFV44_RS13245"
                     /old_locus_tag="KFV44_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(6123..6683)
                     /locus_tag="KFV44_RS13250"
                     /old_locus_tag="KFV44_13260"
     CDS             complement(6123..6683)
                     /locus_tag="KFV44_RS13250"
                     /old_locus_tag="KFV44_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            complement(6934..7500)
                     /gene="qacR"
                     /locus_tag="KFV44_RS13255"
                     /old_locus_tag="KFV44_13265"
     ARG             complement(6938..7500)
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            7679..9223
                     /gene="qac"
                     /locus_tag="KFV44_RS13260"
                     /old_locus_tag="KFV44_13270"
     CDS             7679..9223
                     /gene="qac"
                     /locus_tag="KFV44_RS13260"
                     /old_locus_tag="KFV44_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            9428..9988
                     /locus_tag="KFV44_RS13265"
                     /old_locus_tag="KFV44_13275"
     CDS             9428..9988
                     /locus_tag="KFV44_RS13265"
                     /old_locus_tag="KFV44_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(10307..11068)
                     /locus_tag="KFV44_RS13275"
                     /old_locus_tag="KFV44_13285"
     CDS             complement(10307..11068)
                     /locus_tag="KFV44_RS13275"
                     /old_locus_tag="KFV44_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            11509..12444
                     /locus_tag="KFV44_RS13280"
                     /old_locus_tag="KFV44_13290"
     CDS             11509..12444
                     /locus_tag="KFV44_RS13280"
                     /old_locus_tag="KFV44_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            complement(12669..12908)
                     /locus_tag="KFV44_RS13285"
                     /old_locus_tag="KFV44_13295"
     CDS             complement(12669..12908)
                     /locus_tag="KFV44_RS13285"
                     /old_locus_tag="KFV44_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(12925..13194)
                     /locus_tag="KFV44_RS13290"
                     /old_locus_tag="KFV44_13300"
     CDS             complement(12925..13194)
                     /locus_tag="KFV44_RS13290"
                     /old_locus_tag="KFV44_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     ARG             7680..9220
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          11510..12444
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..1686
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 cctcaacatg cacccgaatg gactaaagat agacagcgtt tatggaatga agttgataaa
       61 gttgaaaaga ataatgcgaa aacaaaaaat cctagattag caaaagaagt cttattaagt
      121 ttgcctaatg attttgatag agaggtacaa actgaattaa ctaaagattt tataaaaagt
      181 gagtttgtag acaaaggaat ggttgctgat atttcaattc atagagatga tataaacaac
      241 ccacacgccc atgttctact tactcaaaga ccgtttaatg cagacggtac ttgggggaaa
      301 aagacaaaaa cgagaacgca atatgatgaa aatggcaatg cgattttaaa taaaaatggt
      361 aataaagtta gaaaacaaga acgatttgcc gactttgatt tcaaagaagt tcgtaagcat
      421 tgggaattaa aattaaatca atactcagaa cgtgaacaaa gtttaagacg atatgatagt
      481 cgttcttttg aagcgcaagg tttagaaaaa attgccgaaa ttccacttac aagagaagaa
      541 tatagattag aacaaaatga aagaaaacgc tgtgaaaaag agggtatcga gtaccaaccc
      601 gttacttatt acggacagaa aaatgaacaa attagaagat ataatcgtgg cgaagtatca
      661 caattattta gtgataaaga gaaagaacaa gcacaaaatg aaattagatt atttattcaa
      721 aatgctaacg aacaagtaag taaaaatgaa caaagttatt ctattttatt tgatagatat
      781 caacgtgatg ttggctattc agaggcgaaa gaaacaatta aaaatacgtt tgacagtgcg
      841 tctacctttg gtcgtaaaat tcaaaatgat ttacttaaaa atgaaattaa gagacgtcaa
      901 ttaaaattct atgctaaaaa ttatgatgaa aattatcagt ttagaaaaga aattgaaagt
      961 aaaggatata gtcaacaaga gtttgttaat actgttgaaa ctaactataa agcgttactc
     1021 aatgagaaga ataaattaga aagtagagag gaaaaacgta aagagattta tcaatcagct
     1081 aaagatgttt atctaactga aagtgaaaaa aatcatctca ttgttcagca aatgtaccct
     1141 aaatctcatc atcattttag ccatgatgaa aaagcattta ttgttgatga ggcacaaaaa
     1201 ggtaatatta taaaagcaac tcatgttcaa tacctgttca aacaaaataa caatattcct
     1261 aatgatgtat tgctagaaga tacttataaa aaagcgagta aagatatttt ctttagtcaa
     1321 aaacatctta aaaatttaga aaaagatagc tttgaatata acgttgagtt gcaatttatt
     1381 aaggaaaaag aaaaagaaat tgatagttat aaagaacata ttgatgatga tttaagaata
     1441 gatataggcg ataaaaactt tgaaaaagtc aaagaccaaa cacctaatga aaaagttcaa
     1501 ttactcatta aacttgagaa atataatcat gtcgatcaag aagaagttat cgaaaaacat
     1561 attaatcaag tagatagaaa tgatcatgac aatcgtattg aacatgaaaa tgaacaaact
     1621 aatcacatta atttaccaac gataaatgct agtgactttc ttatgtacta tgcgaacgaa
     1681 actgaaaagc aaaacaatat taatcatcag aaaaagaaga agaaacaaga gagaaataac
     1741 caaatgagaa gatagtaagc agttgaaatc atacaaagtt cattctttaa taataagaaa
     1801 cttaacccaa tgtggtaaat tagctttggt ttaaagaaaa catttatata cttagataag
     1861 gtacattcta tgtattatca caatgtattt taaaacgtgt ataattatag aaaaaccttg
     1921 atattatgcg attgttcaca tatttcagca taaaaaatac taatgttcat aaatatcgat
     1981 agaaaaaatt gagtttaaat atatcttaaa gttaaggaga aaaataattt ggaaacaaaa
     2041 attggacatc aaatacaaaa gttaagaaaa agtaaaaaca tgtctcaaga aaaattagct
     2101 gaaaagttag gggtatcgag acattctatt tctaattggg aaagagaagt aagtaatccc
     2161 gacttaaaaa caattttaga aatcactaaa ttatttaatg tttctctaaa tcaattaatt
     2221 aaaggagtgg aaatcatgca agttaataaa tatgtttact ctgcattagc attttttcta
     2281 ggaggtttcg gggctcataa gttttatgtt aagaaaaata aaaatgctct tctttattta
     2341 ctattttgtt ggacgggaat accgggtgtg ataggtatgg tagaaggtgt tttaacactt
     2401 atgcgtccga gcgatagtga aaacaatata gaaataaatt aaaccaaaaa taaaaagccg
     2461 atgtatcgga ttttttttag tgtacatgtg aatattaaaa atgatggagt gatttcacat
     2521 gaacacattc gttttcggcg tgattacaaa gcgattagaa gaaataggtt ttaatgtaca
     2581 caaggtgttt aaaatgcccg aaaaacaggc actatttgaa gatttgtacc attatgtgag
     2641 atacgttaaa ccatgcaagg ctgaactaac aatggtggca ttagatagtg atgaattgat
     2701 tcattacttt atatgctgtt acatggaacg ttatgcttat cttttgaact aatgataaaa
     2761 aagacgccta attttaggcg cctttttaat aatctactaa ctacttatta tatctaattt
     2821 tacttgctaa cttagctact gttttattgt cgtagggttt aatatagact gtttcaatat
     2881 tctcctctat tgcttttttt gttatcttat tcatttgact ataatcacta ttttgtgaat
     2941 tttcccattt ataagggata ttattatata cattaattgt tccatcacca ttactgctat
     3001 atgttacaga cccaccagct ctcataggag cttctaattt cacaactttt tctttataaa
     3061 ttgcactatt tttggcttgt ggatttactt ttgagccttt tggtatttct gtaacataaa
     3121 catttttaat accttttaaa tcatttcttg tagatattaa ctgataccaa actcgtgcat
     3181 attcaatttc tttcgagtta aaattattta agtaactttt atttttatca gtagattgtt
     3241 gcttcttatt actatcatta tttttttctt gttttttatt atcatttttt tctttatttt
     3301 gtttattact actttctgaa ttatccgcac tatcagatgt gttagttttt tcgtcaattt
     3361 catttgatgc ttcattatct ttgttttctg aggtgttagt atcactagaa ttattatcat
     3421 tgtgatgttc tgaatcgtta ttattacaac tcactaaaat taccgataaa ctaacaatta
     3481 atataggtaa aatgattttt ttcatattac aactcctttt cagtatttca aattagatta
     3541 tgttaatatt ttttcagcaa gaaaacgtaa attacatgag ttgaaaaata tgtattaaat
     3601 atataataaa ataaccacat gttttacata aacacggtgg ttattttatc aatttttata
     3661 ttgccttttt tctgaatatt aaaagactta tacctaatat tagtagtcca gaagcgccaa
     3721 ttattgacaa tatatcactt tggttgcttt ctcctgtttc tggtaatact tgagagtttg
     3781 aatcttgatt tttcattcct tcagaactct tgtttttatt ttcagttaca ggtgtcaaca
     3841 caccttgagg tgtattaata gttttatttt cttcattagc tatcttttga ctttcatttt
     3901 ttacaatttg attatatttt tctgtggata cactattagg gttaggaaca tgactttcta
     3961 attcttggta ttcttcttga gtcatacaat taggtatcac ccctccgaaa ggtcttttac
     4021 cttgttgagt tctctcatta ttttgtatat cagtactttg tgattgatat tcattaaata
     4081 cacttgaaac tttctcatta tactcgattt gagttaattc ccctgaatct ttctgtttag
     4141 aaagttcact tatttgattt tgtgcagatt gtgggaggga tgaagtattg aagtctacat
     4201 tagcccaacc atctccattt gtatctgcgc tatttggtaa gttctctccg tatacggttt
     4261 tggcaccatc ctcagcattg gctgtcgctg aaaaacctgt gaatgttaat gagctagcca
     4321 ataataaagt tggaagtaat gatcgttttt tcataaaatt atctccttta atagaatttg
     4381 tattttcatt atcacataga tacattaaac ataaaatgac ttatcggaag tgcatatgct
     4441 taacaattca caaatgatat aatataaaag tcgtcgttct acttctgtgc tggtttagca
     4501 tggaaggagg tgacaccatg ttcagcatca tatttgtatc tatagtagct cctattatag
     4561 taggggttat aatcacgttg ttttcacatt ggttgaataa tcgtgataaa caataaagcg
     4621 acgacacagt aataacacca aaaagagccc gataactact ggaatagtta tcgggctcgg
     4681 tgtttaccat gttcaacaca tatttgttat ctaaattata acatcatagt gtggaggaat
     4741 gcaaaattga ataattagta tgttaagtct atactatact gattagtaag tactaattat
     4801 catctttgtt attgttaccc ctttcattgg cttttgaatc taattctttt tgaaattttt
     4861 tgtgtgattt tgaagaagtg tttaaaaaga aataggtcat attatccata cgatgtcctt
     4921 ttataaattc attgataaat tgtttttttg tcattttttt ggttgtatta cgttgttttt
     4981 gcaatctaat gatttcttct tcaatgtata ctcttatcat caaatctaaa ctagatgtct
     5041 gattataagt agtgaataat tttggaacta ataaagtttt attaccatca aaaagaaatg
     5101 gagtgaaatc ataagtttcc catataccgt tagtagagaa ataataatat gtcacagttt
     5161 ctttagaaag aaacgatttc atttctggaa atgactgaat catggattta ttaaaatcaa
     5221 ttataacatt catgattata ttacttgtta aatctgataa tttatcatca ccaaaatttc
     5281 tgattaatgg tgctttttga ggtattgtta atccgtcatc atttaagata atggaaagaa
     5341 tatcttcatt cataaataga ttaatcaatt cagatgctgt agtacctttt cctgtattac
     5401 ctttctgaga aaaacctaag cgtaaatggt gtggttctga aaaatgagag aataattttc
     5461 taaattcatt acgcttttgg gtatcagaaa aatcactaag atgtatatcg ataatttttt
     5521 caaaaaaaac atcaatttgc tcttgtgctc tttgagccaa agtcgcaact tttccaactt
     5581 ctctattaga tattacttta ataaggtttg ggtctatata ttgaagagtg tcttcttcaa
     5641 tagaaatatt taaataatca gttgatttag catcatgatt gaatttggta catactagtg
     5701 tcactctata tcatccttct gtaagttttt tgtttacttc caatatagaa tgatgtatta
     5761 ttgtaattgt cgatttacaa taagttgtta ttctaggtgg aagtttattg cttttgatgt
     5821 atagatagac ttaacctttt cattatagca tactatagaa gaaaatgcaa gcaaaagcat
     5881 ataaacaaca aatgttgttt atatgctttt gcttttttgt taattggaga ggacaaggtg
     5941 aatattaaca gtcaaaatcc agttatgtcg gttaatgaat cgatgaaaat tttcaaagct
     6001 caagtaatgc ttagaaattt aaagctatta agcgagtgga ttgttaaagg ggactgaagt
     6061 taaagacact ttttaagaag atttttcaat taaagggtat attaaaactt taatttagta
     6121 ctttatattt ttcttgactc tttaataatc ctatatacag tagaacgact cactcctgtt
     6181 tgttcagcaa tttcttcacc agacagcttt tgttcatcat ataagaattt gatctctctt
     6241 ttcttatgat ctggtagtga aggtcttcca ccttttctcc ctcttgctct tgcagcttcc
     6301 aaaccttttt tagttcgttc acttaataaa ttagcttcaa gttcagcgaa tgcactcatc
     6361 atggtaaaga acatcttgcc catcgcatct ttcgttgaca cattcatatc tataatatgt
     6421 aaatcaatgc tattttcatc caaccattga gatagctcaa ttaattgttt tgttgtacgt
     6481 ccaagacgat ctaatttata gacaactaga atatctcctt cacgtaaata atctaaacac
     6541 ttatcaagtt ccgttctttt agttttacga ccactcgctt tctcgctaaa gatacgttca
     6601 caaccatatt cctcaagtgt atcaatctgt ccatcaagac tttgatcttg tgttgacaca
     6661 cgtgcataac caattttagc catttgaaca ccaccttttt taatcgtttt atgggtactt
     6721 actaatgaaa tatagttctc gatgtaaata aacacgtctg tcagcatcta aaaactcatg
     6781 attttcgata tttatgatac attgattata acacaagttt atagacttaa atatccaaga
     6841 aaaacctctt aatattaagt taagaggttt tgtgtcaaaa acgaacgtaa tttagaaata
     6901 atatttattg gtatttcaag aatatatata taattattta ctaagtccat ttaaaaaaat
     6961 ttgagagaac ttgttcataa gtttaattct ttcattaata ttttgttcat gtgtaaaggt
     7021 aacaattcca ttaacagcgt ttgctgctat tttactaaca gcattaacat cattaataca
     7081 ccattcgcca tttaaattac cctccttaaa aattacatga taagcatcta tatatttgtt
     7141 ttctaattta ttcatttttt cattaatgct attagtttta taatattcag tgtaaaattc
     7201 aattattgcg ttttgaagtg gataatagta ttcggttgtt aaagatagtt cattatataa
     7261 ataaaatttt tctctattag ttttacattt gatttgttcc tttttccact gttcttgcca
     7321 tttagattct tctatattta aaatttctaa aaatagattt tcttttgttt taaagtgata
     7381 ataaagattc cctttactac tttctgataa tttaacaatt tctccagtag tagtggcatt
     7441 atatccattt tttataaata attcctttgc gacacctagt attttatctt tcaagttcat
     7501 cacgccttta atgagtatta agtactatca atgatagcac aaaaaattca aagaataatt
     7561 gtataacaca atacagccat ttaaatttcg caagattttt tgttgtaata tgtaaaaaaa
     7621 atagattata atccttatag accgatcgca cggtctataa ggattggagg gaacttaaat
     7681 gatttcattt tttacaaaaa ctactgatat gatgacatca aaaaaaagat ggactgcact
     7741 agtagtatta gctgttagtt tgtttgttgt tacaatggat atgacaatat taattatggc
     7801 tttaccggaa ttagtaagag agttagagcc ttctggtacc caacagttat ggatagttga
     7861 tatatactct cttgttttag ctggctttat aattccattg agtgcctttg ctgataaatg
     7921 gggaagaaaa aaagcattat taactggatt tgctttattt ggcctcgttt cattagctat
     7981 atttttcgca gaaagtgcag agttcgtaat agctattcga tttttacttg gtattgcagg
     8041 tgctttaata atgccaacta ctctttcaat gataagagta atttttgaaa accctaaaga
     8101 aagggccact gcattagctg tatggtcaat cgcttcatcg ataggtgctg tttttggacc
     8161 aattatcgga ggagctttac ttgagcaatt ttcatggcac tcggcatttt taattaatgt
     8221 accgtttgcg ataatagcag ttgtagcagg tttattttta ttaccagagt ctaagttatc
     8281 aaaagaaaag tctcactcgt gggatattcc ttctacaatt ttatcaattg caggcatgat
     8341 tggactggta tggagtatca aagaattttc aaaagaagga ctagcagata ttattccatg
     8401 ggttgtaata gtattagcaa ttaccatgat agtgatattt gttaaacgta atttatcaag
     8461 ttctgatcca atgttagacg taagactttt taaaaagaga tcattttcag ctggtacaat
     8521 tgctgcattt atgacaattt ttgcaatggc atctgttttg ttattagctt cacaatggtt
     8581 acaggttgtg gaagaacttt ctccttttaa agctggctta tacctattac ctatggcaat
     8641 aggagatatg gtgtttgcac caattgcacc cggattagcg gcgcgatttg gaccgaaaat
     8701 agtgttacct tccggaattg gaattgcagc cattggcatg tttattatgt atttctttgg
     8761 tcatccatta tcatattcta caatggcttt agcattaatt ttagttggag ctggtatggc
     8821 ttcactagca gttgcatctg ctctaataat gttagaaaca cctacatcaa aagcaggtaa
     8881 tgcagctgct gttgaagagt ctatgtatga ccttggaaat gtttttggtg tagcagtact
     8941 tggtagccta tcttctatgc tttatcgtgt atttttagat atttcatctt tttcatcaaa
     9001 aggtatagtt ggagatttag ctcatgtagc tgaagaatct gtagtgggcg ctgtcgaagt
     9061 agctaaagct acggggataa aacagcttgc aaacgaggct gtaacatcat ttaatgatgc
     9121 ttttgtagca actgctttag taggtgggat tatcatgatt atcatttcaa tagttgtcta
     9181 tttgttaatt cccaaatcac ttgatataac taaacaaaaa tagtactaaa catatatttt
     9241 tagatattgt agattagttt atttttaaaa ttagtaatag ttaaaaagta gaaccattaa
     9301 ttttaaatgg ttctactttt ttattattaa gtaaaattat aaaagacaat ttcttaaatt
     9361 aataatatga tatacctata tctaataatt tattagtatt aataaacctt gaaaggatga
     9421 ttaactgatg aatagagcag atatttttaa aatatttgat gtagaactta atataactat
     9481 atttttagga gcactagtag caactttcat ctattttata agttacatga acaatgtaaa
     9541 tgaattaatt acattatgct taacattagc ttttcctgca tatttattaa aaattagcag
     9601 atcgttcgag gttattttat tagaggaaaa aataaaaaat agtaaaatca aaatctgtga
     9661 caaagataga attgaacgag agatactggt gaaaaagaac ctaatagatg aactattgtt
     9721 tcctaataga gttattaaaa aatatacact gacaatttta atttacagta taggtattat
     9781 tattctttta ctactttgta atatgttaag ttatggaatt ggtgaaaatg tcaattttat
     9841 ctggagatta ctagcttgtt atgtaacgag tgtcacagtg attatagttg ccttgattat
     9901 aatgataatg atatttgatc ttataattgg attactatta aattttcaaa tatttttaaa
     9961 aaataaaaag tatgatgatt ttttataatt attcgattta tataatgaaa aattaaatga
    10021 ttaaacatag aaacctcaaa acaaaccata tttaatagag tttatggcgc cttatacaaa
    10081 taaaggctat gctgttaaag aattatgtag ctatcttcag attaatatgg atgatgttta
    10141 tactattggc gatagaaaat tgatttacct atgtttaata ccctagataa ttcttctgat
    10201 aatttagttt ttgttagagt ttaacataac tgatgattat cagaagtttg attcacatta
    10261 aaaaggcaca acaagttcat gaacttgtgt gcctttttaa ttgcaattag ttaccaaata
    10321 aacgactcca aaatctcttt ttaggtggtt ctacctccct attatcactg gaatcgttct
    10381 tcgttttttc aacaaactta tcctcttttg ttaattcatc tgtactgtca tgcttattct
    10441 cttcagttat atttttatga tttacttcgg ttggttgaca ttctttttgt tgttggttga
    10501 tattttgaga ttcttcagta aaagttgctt cttgtggatt aatattctgt ctattattca
    10561 tcgttgtatc aaaagaatat ttaagctgtc tttcttcttc taattggctt tctagttttt
    10621 gaattttttt attgttttct aaagataata tttgttgatt ctccaacagt ttatttaatg
    10681 tattaacatt gctattttgt tgatctaatt gtttagttag attctcaata tatttttcat
    10741 catttttagc tctagtttca aagatttcta tttgcttttc taattcgttt acttttgttt
    10801 ttaatgtctc agaactattt gaattattat gtatatactt ttcaactttt gcagtatttt
    10861 cattaaataa ttcatttttt actgattctt tattatgagt tgttttctct ttcttagtga
    10921 ttttttctat aatttttaaa taatcattat ctttaatata attaattcca ttttcttttt
    10981 caaaagatat atttaatctt ttagcattat taacaatagt ttgtttagtt acacctaatt
    11041 catcagcaat cattttaata gttttcataa catcccccct taggtaccta atttagtacc
    11101 taaattttac cacctaacaa aatttatttc atcatttagt actaaattta ctatctatta
    11161 actacctaaa tgcctaatta tctatcctta aaatattttc gccacttaat taggtgctaa
    11221 aatagcgaat tatacgtttg gtagttttag gtgtactttt aattacattt aaaactcttt
    11281 atatacgcca ttaaaagtgt taatattact ttataaatat taaaagagtc gatgctattg
    11341 gcgtagcatc gactctcggt aataaaacga ttcgcactcg tttgtttata tatttttttg
    11401 atacttgtat tatatatatc taatcatcta agtgcaagca caaaacatat aacttacgta
    11461 aaaattgttt tattacctca atcccaaaat ggaaatgagg tttttattat gcccaatttt
    11521 gaaaaatata atttatcaca agtaaaaact gaaagatttt atcaactgcc taaatattta
    11581 tttgaagatg catattttaa gaaaatgtct gcagaagcca aaattatgta tgcgttatta
    11641 aaagatcgtt ttgaattatc cctccaaaat gaatgggtag ataaaaataa taatatttac
    11701 tttattttca gtaataaaca tttgtgtgaa tacttaggtt atgcagaaca aaaaattata
    11761 aaattaaaaa aagagttaat aaaatttaat ttactaactc aagaacgtgt tggccttaat
    11821 aaaccaaata gattatacct attaaaacct aattatgaca ttgaagccag tcatatcaag
    11881 gaacttccaa attcacagtt ccagaacaat gaatttggaa gttctagaac tgtgaattta
    11941 agtggtcaag aacttccaaa ttcacagtct aatgatactg attataatga cactgattat
    12001 attaagacta attataatga tatgtatgat ttgaatgata ggaaattaac atattctagt
    12061 aatcatacaa atcattcaaa tcactactat tcaaaattca atgatgaagc tttaaaattt
    12121 caattacttg aagaactacc acaaagtatt caaagttatt taagtaactt ttctgtatct
    12181 gaaattaaac ttattaaatc tgtgttatta aaagccaaaa catccttcaa caattctatt
    12241 gatgcatatt atttattaga agatatggaa tttgaaattg ttaatgttct taagcgtttt
    12301 aaagctacat taattcaaaa aaatgaaacc gttgaagcaa tgcaagggta cttaatgaaa
    12361 tctcttaaat ctgaatttgc agaagtacat acgcttaata aacgacgtga tcatttacca
    12421 atcacttctt tatttaatca ataatacaac ttaataagcc aacaatgatc caattagaga
    12481 atatctttaa ttagatgttc tcttaactgt aagcgaactt tattttcaat gaaatgtaat
    12541 taattaagta agtgggacta aaacttttct tttttagtcc catttttata aacaattatt
    12601 attcattgtt actactatca gcaatttttt ctttttagtg aaacccaagt atttgtaagc
    12661 atgataaatt acttcttttt atatttattg ggtttatttg tattaatgta gttggaatac
    12721 atctttaaca attcttctgt ttcatcagga cttaaatgct cacattcaaa atacttttca
    12781 agcattgcac cttttcgaat cagtctatgt gctctttctt ttctatctaa attttttaat
    12841 ctatgctttc ttaattctgc tttttcaatt tcagcattta tttttttgag atgtttaaaa
    12901 tcattcatcg tatcacttac tattctagtt aattttgtct tctgttaaat tttccttgtt
    12961 ctcgttgctg ctattttgtt cgatatcttt attaaattct ccaatgactt tatccataat
    13021 tttatacaat tcatcactag agttgatcat atttaaatca agtttattta aaaaataacc
    13081 accaatttta ttagatagtt gttcttcttg ttttttcttt tgctctttca gttgtgcaat
    13141 ttgtttttct aattgactta attttgtaat atctgtttgt ttaattttag acattaaaaa
    13201 actcccttag tttatctata taaagcatac cataattcat tgggtaagtc gcacatccat
    13261 ggtataatat aaccagttct taagacactg cactgcccat ttacatgcaa attgaaaatt
    13321 ggcataaaaa tgggcaacca tactggttga tcattatagt tcccacaggg gtaaaaaaac
    13381 agaaaagaga ggaggtaaaa tcatgaataa aggttacttt atgttgagag tgactaacat
    13441 taatcgagca acaaaaaata tcgttgcgtc agcgtcttat cgttctgatg aagcattata
    13501 tagcgaacgt acagatgaaa aaattaagtt tagaaatcat actattaagc ctgaaagcat
    13561 gattttaact
//
