LOCUS       NZ_CP073858            13570 bp    DNA     circular CON 17-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1272014 plasmid p1B1272014,
            complete sequence.
ACCESSION   NZ_CP073858
VERSION     NZ_CP073858.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699884
            Assembly: GCF_019329665.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 341x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019329665.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 06:52:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,637
            CDSs (with protein)               :: 2,637
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 44 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 27 of 83
            Pseudo Genes (multiple problems)  :: 26 of 83
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073858.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1272014"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p1B1272014"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(13239..13570,1..235))
                     /gene="qacR"
                     /locus_tag="KFV47_RS13825"
                     /old_locus_tag="KFV47_13835"
     CDS             complement(join(13239..13570,1..235))
                     /gene="qacR"
                     /locus_tag="KFV47_RS13825"
                     /old_locus_tag="KFV47_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug-binding transcriptional regulator QacR"
                     /protein_id="WP_001807342.1"
                     /translation="MNLKDKILGVAKELFIKNGYNATTTGEIVKLSESSKGNLYYHFKT
                     KENLFLEILNIEESKWQEQWKKEQIKCKTNREKFYLYNELSLTTEYYYPLQNAIIEFYT
                     EYYKTNSINEKMNKLENKYIDAYHVIFKEGNLNGEWCINDVNAVSKIAANAVNGIVTFT
                     HEQNINERIKLMNKFSQIFLNGLSK"
                     /name="qacR"
     gene            414..1958
                     /gene="qac"
                     /locus_tag="KFV47_RS13830"
                     /old_locus_tag="KFV47_13840"
     CDS             414..1958
                     /gene="qac"
                     /locus_tag="KFV47_RS13830"
                     /old_locus_tag="KFV47_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            2163..2723
                     /locus_tag="KFV47_RS13835"
                     /old_locus_tag="KFV47_13845"
     CDS             2163..2723
                     /locus_tag="KFV47_RS13835"
                     /old_locus_tag="KFV47_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(3042..3803)
                     /locus_tag="KFV47_RS13845"
                     /old_locus_tag="KFV47_13855"
     CDS             complement(3042..3803)
                     /locus_tag="KFV47_RS13845"
                     /old_locus_tag="KFV47_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            4244..5179
                     /locus_tag="KFV47_RS13850"
                     /old_locus_tag="KFV47_13860"
     CDS             4244..5179
                     /locus_tag="KFV47_RS13850"
                     /old_locus_tag="KFV47_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            complement(5404..5643)
                     /locus_tag="KFV47_RS13855"
                     /old_locus_tag="KFV47_13865"
     CDS             complement(5404..5643)
                     /locus_tag="KFV47_RS13855"
                     /old_locus_tag="KFV47_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(5660..5929)
                     /locus_tag="KFV47_RS13860"
                     /old_locus_tag="KFV47_13870"
     CDS             complement(5660..5929)
                     /locus_tag="KFV47_RS13860"
                     /old_locus_tag="KFV47_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            6138..8060
                     /locus_tag="KFV47_RS13865"
                     /old_locus_tag="KFV47_13875"
     CDS             6138..8060
                     /locus_tag="KFV47_RS13865"
                     /old_locus_tag="KFV47_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            8334..8747
                     /locus_tag="KFV47_RS14155"
                     /old_locus_tag="KFV47_13880"
     CDS             8334..8747
                     /locus_tag="KFV47_RS14155"
                     /old_locus_tag="KFV47_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            8824..9057
                     /locus_tag="KFV47_RS13875"
                     /old_locus_tag="KFV47_13885"
     CDS             8824..9057
                     /locus_tag="KFV47_RS13875"
                     /old_locus_tag="KFV47_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(9106..9810)
                     /locus_tag="KFV47_RS13880"
                     /old_locus_tag="KFV47_13890"
     CDS             complement(9106..9810)
                     /locus_tag="KFV47_RS13880"
                     /old_locus_tag="KFV47_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(9961..10659)
                     /locus_tag="KFV47_RS13885"
                     /old_locus_tag="KFV47_13895"
     CDS             complement(9961..10659)
                     /locus_tag="KFV47_RS13885"
                     /old_locus_tag="KFV47_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            10823..10921
                     /locus_tag="KFV47_RS13890"
                     /old_locus_tag="KFV47_13900"
     CDS             10823..10921
                     /locus_tag="KFV47_RS13890"
                     /old_locus_tag="KFV47_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(11098..12009)
                     /locus_tag="KFV47_RS13895"
                     /old_locus_tag="KFV47_13905"
     CDS             complement(11098..12009)
                     /locus_tag="KFV47_RS13895"
                     /old_locus_tag="KFV47_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(12428..12988)
                     /locus_tag="KFV47_RS13900"
                     /old_locus_tag="KFV47_13910"
     CDS             complement(12428..12988)
                     /locus_tag="KFV47_RS13900"
                     /old_locus_tag="KFV47_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     ARG             415..1955
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          4245..5179
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6139..7991
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 atttttctct attagtttta catttgattt gttccttttt ccactgttct tgccatttag
       61 attcttctat atttaaaatt tctaaaaata gattttcttt tgttttaaag tgataataaa
      121 gattcccttt actactttct gataatttaa caatttctcc agtagtagtg gcattatatc
      181 cattttttat aaataattcc tttgcgacac ctagtatttt atctttcaag ttcatcacgc
      241 ctttaatgag tattaagtac tatcaatgat agcacaaaaa attcaaagaa taattgtata
      301 acacaataca gccatttaaa tttcgcaaga ttttttgttg taatatgtaa aaaaaataga
      361 ttataatcct tatagaccga tcgcacggtc tataaggatt ggagggaact taaatgattt
      421 cattttttac aaaaactact gatatgatga catcaaaaaa aagatggact gcactagtag
      481 tattagctgt tagtttgttt gttgttacaa tggatatgac aatattaatt atggctttac
      541 cggaattagt aagagagtta gagccttctg gtacccaaca gttatggata gttgatatat
      601 actctcttgt tttagctggc tttataattc cattgagtgc ctttgctgat aaatggggaa
      661 gaaaaaaagc attattaact ggatttgctt tatttggcct cgtttcatta gctatatttt
      721 tcgcagaaag tgcagagttc gtaatagcta ttcgattttt acttggtatt gcaggtgctt
      781 taataatgcc aactactctt tcaatgataa gagtaatttt tgaaaaccct aaagaaaggg
      841 ccactgcatt agctgtatgg tcaatcgctt catcgatagg tgctgttttt ggaccaatta
      901 tcggaggagc tttacttgag caattttcat ggcactcggc atttttaatt aatgtaccgt
      961 ttgcgataat agcagttgta gcaggtttat ttttattacc agagtctaag ttatcaaaag
     1021 aaaagtctca ctcgtgggat attccttcta caattttatc aattgcaggc atgattggac
     1081 tggtatggag tatcaaagaa ttttcaaaag aaggactagc agatattatt ccatgggttg
     1141 taatagtatt agcaattacc atgatagtga tatttgttaa acgtaattta tcaagttctg
     1201 atccaatgtt agacgtaaga ctttttaaaa agagatcatt ttcagctggt acaattgctg
     1261 catttatgac aatttttgca atggcatctg ttttgttatt agcttcacaa tggttacagg
     1321 ttgtggaaga actttctcct tttaaagctg gcttatacct attacctatg gcaataggag
     1381 atatggtgtt tgcaccaatt gcacccggat tagcggcgcg atttggaccg aaaatagtgt
     1441 taccttccgg aattggaatt gcagccattg gcatgtttat tatgtatttc tttggtcatc
     1501 cattatcata ttctacaatg gctttagcat taattttagt tggagctggt atggcttcac
     1561 tagcagttgc atctgctcta ataatgttag aaacacctac atcaaaagca ggtaatgcag
     1621 ctgctgttga agagtctatg tatgaccttg gaaatgtttt tggtgtagca gtacttggta
     1681 gcctatcttc tatgctttat cgtgtatttt tagatatttc atctttttca tcaaaaggta
     1741 tagttggaga tttagctcat gtagctgaag aatctgtagt gggcgctgtc gaagtagcta
     1801 aagctacggg gataaaacag cttgcaaacg aggctgtaac atcatttaat gatgcttttg
     1861 tagcaactgc tttagtaggt gggattatca tgattatcat ttcaatagtt gtctatttgt
     1921 taattcccaa atcacttgat ataactaaac aaaaatagta ctaaacatat atttttagat
     1981 attgtagatt agtttatttt taaaattagt aatagttaaa aagtagaacc attaatttta
     2041 aatggttcta cttttttatt attaagtaaa attataaaag acaatttctt aaattaataa
     2101 tatgatatac ctatatctaa taatttatta gtattaataa accttgaaag gatgattaac
     2161 tgatgaatag agcagatatt tttaaaatat ttgatgtaga acttaatata actatatttt
     2221 taggagcact agtagcaact ttcatctatt ttataagtta catgaacaat gtaaatgaat
     2281 taattacatt atgcttaaca ttagcttttc ctgcatattt attaaaaatt agcagatcgt
     2341 tcgaggttat tttattagag gaaaaaataa aaaatagtaa aatcaaaatc tgtgacaaag
     2401 atagaattga acgagagata ctggtgaaaa agaacctaat agatgaacta ttgtttccta
     2461 atagagttat taaaaaatat acactgacaa ttttaattta cagtataggt attattattc
     2521 ttttactact ttgtaatatg ttaagttatg gaattggtga aaatgtcaat tttatctgga
     2581 gattactagc ttgttatgta acgagtgtca cagtgattat agttgccttg attataatga
     2641 taatgatatt tgatcttata attggattac tattaaattt tcaaatattt ttaaaaaata
     2701 aaaagtatga tgatttttta taattattcg atttatataa tgaaaaatta aatgattaaa
     2761 catagaaacc tcaaaacaaa ccatatttaa tagagtttat ggcgccttat acaaataaag
     2821 gctatgctgt taaagaatta tgtagctatc ttcagattaa tatggatgat gtttatacta
     2881 ttggcgatag aaaattgatt tacctatgtt taatacccta gataattctt ctgataattt
     2941 agtttttgtt agagtttaac ataactgatg attatcagaa gtttgattca cattaaaaag
     3001 gcacaacaag ttcatgaact tgtgtgcctt tttaattgca attagttacc aaataaacga
     3061 ctccaaaatc tctttttagg tggttctacc tccctattat cactggaatc gttcttcgtt
     3121 ttttcaacaa acttatcctc ttttgttaat tcatctgtac tgtcatgctt attctcttca
     3181 gttatatttt tatgatttac ttcggttggt tgacattctt tttgttgttg gttgatattt
     3241 tgagattctt cagtaaaagt tgcttcttgt ggattaatat tctgtctatt attcatcgtt
     3301 gtatcaaaag aatatttaag ctgtctttct tcttctaatt ggctttctag tttttgaatt
     3361 tttttattgt tttctaaaga taatatttgt tgattctcca acagtttatt taatgtatta
     3421 acattgctat tttgttgatc taattgttta gttagattct caatatattt ttcatcattt
     3481 ttagctctag tttcaaagat ttctatttgc ttttctaatt cgtttacttt tgtttttaat
     3541 gtctcagaac tatttgaatt attatgtata tacttttcaa cttttgcagt attttcatta
     3601 aataattcat tttttactga ttctttatta tgagttgttt tctctttctt agtgattttt
     3661 tctataattt ttaaataatc attatcttta atataattaa ttccattttc tttttcaaaa
     3721 gatatattta atcttttagc attattaaca atagtttgtt tagttacacc taattcatca
     3781 gcaatcattt taatagtttt cataacatcc ccccttaggt acctaattta gtacctaaat
     3841 tttaccacct aacaaaattt atttcatcat ttagtactaa atttactatc tattaactac
     3901 ctaaatgcct aattatctat ccttaaaata ttttcgccac ttaattaggt gctaaaatag
     3961 cgaattatac gtttggtagt tttaggtgta cttttaatta catttaaaac tctttatata
     4021 cgccattaaa agtgttaata ttactttata aatattaaaa gagtcgatgc tattggcgta
     4081 gcatcgactc tcggtaataa aacgattcgc actcgtttgt ttatatattt ttttgatact
     4141 tgtattatat atatctaatc atctaagtgc aagcacaaaa catataactt acgtaaaaat
     4201 tgttttatta cctcaatccc aaaatggaaa tgaggttttt attatgccca attttgaaaa
     4261 atataattta tcacaagtaa aaactgaaag attttatcaa ctgcctaaat atttatttga
     4321 agatgcatat tttaagaaaa tgtctgcaga agccaaaatt atgtatgcgt tattaaaaga
     4381 tcgttttgaa ttatccctcc aaaatgaatg ggtagataaa aataataata tttactttat
     4441 tttcagtaat aaacatttgt gtgaatactt aggttatgca gaacaaaaaa ttataaaatt
     4501 aaaaaaagag ttaataaaat ttaatttact aactcaagaa cgtgttggcc ttaataaacc
     4561 aaatagatta tacctattaa aacctaatta tgacattgaa gccagtcata tcaaggaact
     4621 tccaaattca cagttccaga acaatgaatt tggaagttct agaactgtga atttaagtgg
     4681 tcaagaactt ccaaattcac agtctaatga tactgattat aatgacactg attatattaa
     4741 gactaattat aatgatatgt atgatttgaa tgataggaaa ttaacatatt ctagtaatca
     4801 tacaaatcat tcaaatcact actattcaaa attcaatgat gaagctttaa aatttcaatt
     4861 acttgaagaa ctaccacaaa gtattcaaag ttatttaagt aacttttctg tatctgaaat
     4921 taaacttatt aaatctgtgt tattaaaagc caaaacatcc ttcaacaatt ctattgatgc
     4981 atattattta ttagaagata tggaatttga aattgttaat gttcttaagc gttttaaagc
     5041 tacattaatt caaaaaaatg aaaccgttga agcaatgcaa gggtacttaa tgaaatctct
     5101 taaatctgaa tttgcagaag tacatacgct taataaacga cgtgatcatt taccaatcac
     5161 ttctttattt aatcaataat acaacttaat aagccaacaa tgatccaatt agagaatatc
     5221 tttaattaga tgttctctta actgtaagcg aactttattt tcaatgaaat gtaattaatt
     5281 aagtaagtgg gactaaaact tttctttttt agtcccattt ttataaacaa ttattattca
     5341 ttgttactac tatcagcaat tttttctttt tagtgaaacc caagtatttg taagcatgat
     5401 aaattacttc tttttatatt tattgggttt atttgtatta atgtagttgg aatacatctt
     5461 taacaattct tctgtttcat caggacttaa atgctcacat tcaaaatact tttcaagcat
     5521 tgcacctttt cgaatcagtc tatgtgctct ttcttttcta tctaaatttt ttaatctatg
     5581 ctttcttaat tctgcttttt caatttcagc atttattttt ttgagatgtt taaaatcatt
     5641 catcgtatca cttactattc tagttaattt tgtcttctgt taaattttcc ttgttctcgt
     5701 tgctgctatt ttgttcgata tctttattaa attctccaat gactttatcc ataattttat
     5761 acaattcatc actagagttg atcatattta aatcaagttt atttaaaaaa taaccaccaa
     5821 ttttattaga tagttgttct tcttgttttt tcttttgctc tttcagttgt gcaatttgtt
     5881 tttctaattg acttaatttt gtaatatctg tttgtttaat tttagacatt aaaaaactcc
     5941 cttagtttat ctatataaag cataccataa ttcattgggt aagtcgcaca tccatggtat
     6001 aatataacca gttcttaaga cactgcactg cccatttaca tgcaaattga aaattggcat
     6061 aaaaatgggc aaccatactg gttgatcatt atagttccca caggggtaaa aaaacagaaa
     6121 agagaggagg taaaatcatg aataaaggtt actttatgtt gagagtgact aacattaatc
     6181 gagcaacaaa aaatatcgtt gcgtcagcgt cttatcgttc tgatgaagca ttatatagcg
     6241 aacgtacaga tgaaaaaatt aagtttagaa atcatactat taagcctgaa agcatgattt
     6301 taactcctca acatgcaccc gaatggacta aagatagaca gcgtttatgg aatgaagttg
     6361 ataaagttga aaagaataat gcgaaaacaa aaaatcctag attagcaaaa gaagtcttat
     6421 taagtttgcc taatgatttt gatagagagg tacaaactga attaactaaa gattttataa
     6481 aaagtgagtt tgtagacaaa ggaatggttg ctgatatttc aattcataga gatgatataa
     6541 acaacccaca cgcccatgtt ctacttactc aaagaccgtt taatgcagac ggtacttggg
     6601 ggaaaaagac aaaaacgaga acgcaatatg atgaaaatgg caatgcgatt ttaaataaaa
     6661 atggtaataa agttagaaaa caagaacgat ttgccgactt tgatttcaaa gaagttcgta
     6721 agcattggga attaaaatta aatcaatact cagaacgtga acaaagttta agacgatatg
     6781 atagtcgttc ttttgaagcg caaggtttag aaaaaattgc cgaaattcca cttacaagag
     6841 aagaatatag attagaacaa aatgaaagaa aacgctgtga aaaagagggt atcgagtacc
     6901 aacccgttac ttattacgga cagaaaaatg aacaaattag aagatataat cgtggcgaag
     6961 tatcacaatt atttagtgat aaagagaaag aacaagcaca aaatgaaatt agattattta
     7021 ttcaaaatgc taacgaacaa gtaagtaaaa atgaacaaag ttattctatt ttatttgata
     7081 gatatcaacg tgatgttggc tattcagagg cgaaagaaac aattaaaaat acgtttgaca
     7141 gtgcgtctac ctttggtcgt aaaattcaaa atgatttact taaaaatgaa attaagagac
     7201 gtcaattaaa attctatgct aaaaattatg atgaaaatta tcagtttaga aaagaaattg
     7261 aaagtaaagg atatagtcaa caagagtttg ttaatactgt tgaaactaac tataaagcgt
     7321 tactcaatga gaagaataaa ttagaaagta gagaggaaaa acgtaaagag atttatcaat
     7381 cagctaaaga tgtttatcta actgaaagtg aaaaaaatca tctcattgtt cagcaaatgt
     7441 accctaaatc tcatcatcat tttagccatg atgaaaaagc atttattgtt gatgaggcac
     7501 aaaaaggtaa tattataaaa gcaactcatg ttcaatacct gttcaaacaa aataacaata
     7561 ttcctaatga tgtattgcta gaagatactt ataaaaaagc gagtaaagat attttcttta
     7621 gtcaaaaaca tcttaaaaat ttagaaaaag atagctttga atataacgtt gagttgcaat
     7681 ttattaagga aaaagaaaaa gaaattgata gttataaaga acatattgat gatgatttaa
     7741 gaatagatat aggcgataaa aactttgaaa aagtcaaaga ccaaacacct aatgaaaaag
     7801 ttcaattact cattaaactt gagaaatata atcatgtcga tcaagaagaa gttatcgaaa
     7861 aacatattaa tcaagtagat agaaatgatc atgacaatcg tattgaacat gaaaatgaac
     7921 aaactaatca cattaattta ccaacgataa atgctagtga ctttcttatg tactatgcga
     7981 acgaaactga aaagcaaaac aatattaatc atcagaaaaa gaagaagaaa caagagagaa
     8041 ataaccaaat gagaagatag taagcagttg aaatcataca aagttcattc tttaataata
     8101 agaaacttaa cccaatgtgg taaattagct ttggtttaaa gaaaacattt atatacttag
     8161 ataaggtaca ttctatgtat tatcacaatg tattttaaaa cgtgtataat tatagaaaaa
     8221 ccttgatatt atgcgattgt tcacatattt cagcataaaa aatactaatg ttcataaata
     8281 tcgatagaaa aaattgagtt taaatatatc ttaaagttaa ggagaaaaat aatttggaaa
     8341 caaaaattgg acatcaaata caaaagttaa gaaaaagtaa aaacatgtct caagaaaaat
     8401 tagctgaaaa gttaggggta tcgagacatt ctatttctaa ttgggaaaga gaagtaagta
     8461 atcccgactt aaaaacaatt ttagaaatca ctaaattatt taatgtttct ctaaatcaat
     8521 taattaaagg agtggaaatc atgcaagtta ataaatatgt ttactctgca ttagcatttt
     8581 ttctaggagg tttcggggct cataagtttt atgttaagaa aaataaaaat gctcttcttt
     8641 atttactatt ttgttggacg ggaataccgg gtgtgatagg tatggtagaa ggtgttttaa
     8701 cacttatgcg tccgagcgat agtgaaaaca atatagaaat aaattaaacc aaaaataaaa
     8761 agccgatgta tcggattttt tttagtgtac atgtgaatat taaaaatgat ggagtgattt
     8821 cacatgaaca cattcgtttt cggcgtgatt acaaagcgat tagaagaaat aggttttaat
     8881 gtacacaagg tgtttaaaat gcccgaaaaa caggcactat ttgaagattt gtaccattat
     8941 gtgagatacg ttaaaccatg caaggctgaa ctaacaatgg tggcattaga tagtgatgaa
     9001 ttgattcatt actttatatg ctgttacatg gaacgttatg cttatctttt gaactaatga
     9061 taaaaaagac gcctaatttt aggcgccttt ttaataatct actaactact tattatatct
     9121 aattttactt gctaacttag ctactgtttt attgtcgtag ggtttaatat agactgtttc
     9181 aatattctcc tctattgctt tttttgttat cttattcatt tgactataat cactattttg
     9241 tgaattttcc catttataag ggatattatt atatacatta attgttccat caccattact
     9301 gctatatgtt acagacccac cagctctcat aggagcttct aatttcacaa ctttttcttt
     9361 ataaattgca ctatttttgg cttgtggatt tacttttgag ccttttggta tttctgtaac
     9421 ataaacattt ttaatacctt ttaaatcatt tcttgtagat attaactgat accaaactcg
     9481 tgcatattca atttctttcg agttaaaatt atttaagtaa cttttatttt tatcagtaga
     9541 ttgttgcttc ttattactat cattattttt ttcttgtttt ttattatcat ttttttcttt
     9601 attttgttta ttactacttt ctgaattatc cgcactatca gatgtgttag ttttttcgtc
     9661 aatttcattt gatgcttcat tatctttgtt ttctgaggtg ttagtatcac tagaattatt
     9721 atcattgtga tgttctgaat cgttattatt acaactcact aaaattaccg ataaactaac
     9781 aattaatata ggtaaaatga tttttttcat attacaactc cttttcagta tttcaaatta
     9841 gattatgtta atattttttc agcaagaaaa cgtaaattac atgagttgaa aaatatgtat
     9901 taaatatata ataaaataac cacatgtttt acataaacac ggtggttatt ttatcaattt
     9961 ttatattgcc ttttttctga atattaaaag acttatacct aatattagta gtccagaagc
    10021 gccaattatt gacaatatat cactttggtt gctttctcct gtttctggta atacttgaga
    10081 gtttgaatct tgatttttca ttccttcaga actcttgttt ttattttcag ttacaggtgt
    10141 caacacacct tgaggtgtat taatagtttt attttcttca ttagctatct tttgactttc
    10201 attttttaca atttgattat atttttctgt ggatacacta ttagggttag gaacatgact
    10261 ttctaattct tggtattctt cttgagtcat acaattaggt atcacccctc cgaaaggtct
    10321 tttaccttgt tgagttctct cattattttg tatatcagta ctttgtgatt gatattcatt
    10381 aaatacactt gaaactttct cattatactc gatttgagtt aattcccctg aatctttctg
    10441 tttagaaagt tcacttattt gattttgtgc agattgtggg agggatgaag tattgaagtc
    10501 tacattagcc caaccatctc catttgtatc tgcgctattt ggtaagttct ctccgtatac
    10561 ggttttggca ccatcctcag cattggctgt cgctgaaaaa cctgtgaatg ttaatgagct
    10621 agccaataat aaagttggaa gtaatgatcg ttttttcata aaattatctc ctttaataga
    10681 atttgtattt tcattatcac atagatacat taaacataaa atgacttatc ggaagtgcat
    10741 atgcttaaca attcacaaat gatataatat aaaagtcgtc gttctacttc tgtgctggtt
    10801 tagcatggaa ggaggtgaca ccatgttcag catcatattt gtatctatag tagctcctat
    10861 tatagtaggg gttataatca cgttgttttc acattggttg aataatcgtg ataaacaata
    10921 aagcgacgac acagtaataa caccaaaaag agcccgataa ctactggaat agttatcggg
    10981 ctcggtgttt accatgttca acacatattt gttatctaaa ttataacatc atagtgtgga
    11041 ggaatgcaaa attgaataat tagtatgtta agtctatact atactgatta gtaagtacta
    11101 attatcatct ttgttattgt tacccctttc attggctttt gaatctaatt ctttttgaaa
    11161 ttttttgtgt gattttgaag aagtgtttaa aaagaaatag gtcatattat ccatacgatg
    11221 tccttttata aattcattga taaattgttt ttttgtcatt tttttggttg tattacgttg
    11281 tttttgcaat ctaatgattt cttcttcaat gtatactctt atcatcaaat ctaaactaga
    11341 tgtctgatta taagtagtga ataattttgg aactaataaa gttttattac catcaaaaag
    11401 aaatggagtg aaatcataag tttcccatat accgttagta gagaaataat aatatgtcac
    11461 agtttcttta gaaagaaacg atttcatttc tggaaatgac tgaatcatgg atttattaaa
    11521 atcaattata acattcatga ttatattact tgttaaatct gataatttat catcaccaaa
    11581 atttctgatt aatggtgctt tttgaggtat tgttaatccg tcatcattta agataatgga
    11641 aagaatatct tcattcataa atagattaat caattcagat gctgtagtac cttttcctgt
    11701 attacctttc tgagaaaaac ctaagcgtaa atggtgtggt tctgaaaaat gagagaataa
    11761 ttttctaaat tcattacgct tttgggtatc agaaaaatca ctaagatgta tatcgataat
    11821 tttttcaaaa aaaacatcaa tttgctcttg tgctctttga gccaaagtcg caacttttcc
    11881 aacttctcta ttagatatta ctttaataag gtttgggtct atatattgaa gagtgtcttc
    11941 ttcaatagaa atatttaaat aatcagttga tttagcatca tgattgaatt tggtacatac
    12001 tagtgtcact ctatatcatc cttctgtaag ttttttgttt acttccaata tagaatgatg
    12061 tattattgta attgtcgatt tacaataagt tgttattcta ggtggaagtt tattgctttt
    12121 gatgtataga tagacttaac cttttcatta tagcatacta tagaagaaaa tgcaagcaaa
    12181 agcatataaa caacaaatgt tgtttatatg cttttgcttt tttgttaatt ggagaggaca
    12241 aggtgaatat taacagtcaa aatccagtta tgtcggttaa tgaatcgatg aaaattttca
    12301 aagctcaagt aatgcttaga aatttaaagc tattaagcga gtggattgtt aaaggggact
    12361 gaagttaaag acacttttta agaagatttt tcaattaaag ggtatattaa aactttaatt
    12421 tagtacttta tatttttctt gactctttaa taatcctata tacagtagaa cgactcactc
    12481 ctgtttgttc agcaatttct tcaccagaca gcttttgttc atcatataag aatttgatct
    12541 ctcttttctt atgatctggt agtgaaggtc ttccaccttt tctccctctt gctcttgcag
    12601 cttccaaacc ttttttagtt cgttcactta ataaattagc ttcaagttca gcgaatgcac
    12661 tcatcatggt aaagaacatc ttgcccatcg catctttcgt tgacacattc atatctataa
    12721 tatgtaaatc aatgctattt tcatccaacc attgagatag ctcaattaat tgttttgttg
    12781 tacgtccaag acgatctaat ttatagacaa ctagaatatc tccttcacgt aaataatcta
    12841 aacacttatc aagttccgtt cttttagttt tacgaccact cgctttctcg ctaaagatac
    12901 gttcacaacc atattcctca agtgtatcaa tctgtccatc aagactttga tcttgtgttg
    12961 acacacgtgc ataaccaatt ttagccattt gaacaccacc ttttttaatc gttttatggg
    13021 tacttactaa tgaaatatag ttctcgatgt aaataaacac gtctgtcagc atctaaaaac
    13081 tcatgatttt cgatatttat gatacattga ttataacaca agtttataga cttaaatatc
    13141 caagaaaaac ctcttaatat taagttaaga ggttttgtgt caaaaacgaa cgtaatttag
    13201 aaataatatt tattggtatt tcaagaatat atatataatt atttactaag tccatttaaa
    13261 aaaatttgag agaacttgtt cataagttta attctttcat taatattttg ttcatgtgta
    13321 aaggtaacaa ttccattaac agcgtttgct gctattttac taacagcatt aacatcatta
    13381 atacaccatt cgccatttaa attaccctcc ttaaaaatta catgataagc atctatatat
    13441 ttgttttcta atttattcat tttttcatta atgctattag ttttataata ttcagtgtaa
    13501 aattcaatta ttgcgttttg aagtggataa tagtattcgg ttgttaaaga tagttcatta
    13561 tataaataaa
//
