LOCUS       NZ_CP073854            13570 bp    DNA     circular CON 17-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1269488 plasmid p2B1269488,
            complete sequence.
ACCESSION   NZ_CP073854
VERSION     NZ_CP073854.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699883
            Assembly: GCF_019329645.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 436x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019329645.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 06:57:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,625
            Genes (coding)                    :: 2,545
            CDSs (with protein)               :: 2,545
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 43 of 80
            Pseudo Genes (incomplete)         :: 43 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 24 of 80
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073854.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1269488"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p2B1269488"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            join(13392..13570,1..1744)
                     /locus_tag="KFV48_RS00005"
                     /old_locus_tag="KFV48_00005"
     CDS             join(13392..13570,1..1744)
                     /locus_tag="KFV48_RS00005"
                     /old_locus_tag="KFV48_00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            2018..2431
                     /locus_tag="KFV48_RS13610"
                     /old_locus_tag="KFV48_00010"
     CDS             2018..2431
                     /locus_tag="KFV48_RS13610"
                     /old_locus_tag="KFV48_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            2508..2741
                     /locus_tag="KFV48_RS00015"
                     /old_locus_tag="KFV48_00015"
     CDS             2508..2741
                     /locus_tag="KFV48_RS00015"
                     /old_locus_tag="KFV48_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(2790..3494)
                     /locus_tag="KFV48_RS00020"
                     /old_locus_tag="KFV48_00020"
     CDS             complement(2790..3494)
                     /locus_tag="KFV48_RS00020"
                     /old_locus_tag="KFV48_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(3645..4343)
                     /locus_tag="KFV48_RS00025"
                     /old_locus_tag="KFV48_00025"
     CDS             complement(3645..4343)
                     /locus_tag="KFV48_RS00025"
                     /old_locus_tag="KFV48_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            4507..4605
                     /locus_tag="KFV48_RS00030"
                     /old_locus_tag="KFV48_00030"
     CDS             4507..4605
                     /locus_tag="KFV48_RS00030"
                     /old_locus_tag="KFV48_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(4782..5693)
                     /locus_tag="KFV48_RS00035"
                     /old_locus_tag="KFV48_00035"
     CDS             complement(4782..5693)
                     /locus_tag="KFV48_RS00035"
                     /old_locus_tag="KFV48_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(6112..6672)
                     /locus_tag="KFV48_RS00040"
                     /old_locus_tag="KFV48_00040"
     CDS             complement(6112..6672)
                     /locus_tag="KFV48_RS00040"
                     /old_locus_tag="KFV48_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            complement(6923..7489)
                     /gene="qacR"
                     /locus_tag="KFV48_RS00045"
                     /old_locus_tag="KFV48_00045"
     ARG             complement(6927..7489)
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            7668..9212
                     /gene="qac"
                     /locus_tag="KFV48_RS00050"
                     /old_locus_tag="KFV48_00050"
     CDS             7668..9212
                     /gene="qac"
                     /locus_tag="KFV48_RS00050"
                     /old_locus_tag="KFV48_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            9417..9977
                     /locus_tag="KFV48_RS00055"
                     /old_locus_tag="KFV48_00055"
     CDS             9417..9977
                     /locus_tag="KFV48_RS00055"
                     /old_locus_tag="KFV48_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(10296..11057)
                     /locus_tag="KFV48_RS00065"
                     /old_locus_tag="KFV48_00065"
     CDS             complement(10296..11057)
                     /locus_tag="KFV48_RS00065"
                     /old_locus_tag="KFV48_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            11498..12433
                     /locus_tag="KFV48_RS00070"
                     /old_locus_tag="KFV48_00070"
     CDS             11498..12433
                     /locus_tag="KFV48_RS00070"
                     /old_locus_tag="KFV48_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            complement(12658..12897)
                     /locus_tag="KFV48_RS00075"
                     /old_locus_tag="KFV48_00075"
     CDS             complement(12658..12897)
                     /locus_tag="KFV48_RS00075"
                     /old_locus_tag="KFV48_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(12914..13183)
                     /locus_tag="KFV48_RS00080"
                     /old_locus_tag="KFV48_00080"
     CDS             complement(12914..13183)
                     /locus_tag="KFV48_RS00080"
                     /old_locus_tag="KFV48_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     ARG             7669..9209
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          11499..12433
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3..1675
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 acccgaatgg actaaagata gacagcgttt atggaatgaa gttgataaag ttgaaaagaa
       61 taatgcgaaa acaaaaaatc ctagattagc aaaagaagtc ttattaagtt tgcctaatga
      121 ttttgataga gaggtacaaa ctgaattaac taaagatttt ataaaaagtg agtttgtaga
      181 caaaggaatg gttgctgata tttcaattca tagagatgat ataaacaacc cacacgccca
      241 tgttctactt actcaaagac cgtttaatgc agacggtact tgggggaaaa agacaaaaac
      301 gagaacgcaa tatgatgaaa atggcaatgc gattttaaat aaaaatggta ataaagttag
      361 aaaacaagaa cgatttgccg actttgattt caaagaagtt cgtaagcatt gggaattaaa
      421 attaaatcaa tactcagaac gtgaacaaag tttaagacga tatgatagtc gttcttttga
      481 agcgcaaggt ttagaaaaaa ttgccgaaat tccacttaca agagaagaat atagattaga
      541 acaaaatgaa agaaaacgct gtgaaaaaga gggtatcgag taccaacccg ttacttatta
      601 cggacagaaa aatgaacaaa ttagaagata taatcgtggc gaagtatcac aattatttag
      661 tgataaagag aaagaacaag cacaaaatga aattagatta tttattcaaa atgctaacga
      721 acaagtaagt aaaaatgaac aaagttattc tattttattt gatagatatc aacgtgatgt
      781 tggctattca gaggcgaaag aaacaattaa aaatacgttt gacagtgcgt ctacctttgg
      841 tcgtaaaatt caaaatgatt tacttaaaaa tgaaattaag agacgtcaat taaaattcta
      901 tgctaaaaat tatgatgaaa attatcagtt tagaaaagaa attgaaagta aaggatatag
      961 tcaacaagag tttgttaata ctgttgaaac taactataaa gcgttactca atgagaagaa
     1021 taaattagaa agtagagagg aaaaacgtaa agagatttat caatcagcta aagatgttta
     1081 tctaactgaa agtgaaaaaa atcatctcat tgttcagcaa atgtacccta aatctcatca
     1141 tcattttagc catgatgaaa aagcatttat tgttgatgag gcacaaaaag gtaatattat
     1201 aaaagcaact catgttcaat acctgttcaa acaaaataac aatattccta atgatgtatt
     1261 gctagaagat acttataaaa aagcgagtaa agatattttc tttagtcaaa aacatcttaa
     1321 aaatttagaa aaagatagct ttgaatataa cgttgagttg caatttatta aggaaaaaga
     1381 aaaagaaatt gatagttata aagaacatat tgatgatgat ttaagaatag atataggcga
     1441 taaaaacttt gaaaaagtca aagaccaaac acctaatgaa aaagttcaat tactcattaa
     1501 acttgagaaa tataatcatg tcgatcaaga agaagttatc gaaaaacata ttaatcaagt
     1561 agatagaaat gatcatgaca atcgtattga acatgaaaat gaacaaacta atcacattaa
     1621 tttaccaacg ataaatgcta gtgactttct tatgtactat gcgaacgaaa ctgaaaagca
     1681 aaacaatatt aatcatcaga aaaagaagaa gaaacaagag agaaataacc aaatgagaag
     1741 atagtaagca gttgaaatca tacaaagttc attctttaat aataagaaac ttaacccaat
     1801 gtggtaaatt agctttggtt taaagaaaac atttatatac ttagataagg tacattctat
     1861 gtattatcac aatgtatttt aaaacgtgta taattataga aaaaccttga tattatgcga
     1921 ttgttcacat atttcagcat aaaaaatact aatgttcata aatatcgata gaaaaaattg
     1981 agtttaaata tatcttaaag ttaaggagaa aaataatttg gaaacaaaaa ttggacatca
     2041 aatacaaaag ttaagaaaaa gtaaaaacat gtctcaagaa aaattagctg aaaagttagg
     2101 ggtatcgaga cattctattt ctaattggga aagagaagta agtaatcccg acttaaaaac
     2161 aattttagaa atcactaaat tatttaatgt ttctctaaat caattaatta aaggagtgga
     2221 aatcatgcaa gttaataaat atgtttactc tgcattagca ttttttctag gaggtttcgg
     2281 ggctcataag ttttatgtta agaaaaataa aaatgctctt ctttatttac tattttgttg
     2341 gacgggaata ccgggtgtga taggtatggt agaaggtgtt ttaacactta tgcgtccgag
     2401 cgatagtgaa aacaatatag aaataaatta aaccaaaaat aaaaagccga tgtatcggat
     2461 tttttttagt gtacatgtga atattaaaaa tgatggagtg atttcacatg aacacattcg
     2521 ttttcggcgt gattacaaag cgattagaag aaataggttt taatgtacac aaggtgttta
     2581 aaatgcccga aaaacaggca ctatttgaag atttgtacca ttatgtgaga tacgttaaac
     2641 catgcaaggc tgaactaaca atggtggcat tagatagtga tgaattgatt cattacttta
     2701 tatgctgtta catggaacgt tatgcttatc ttttgaacta atgataaaaa agacgcctaa
     2761 ttttaggcgc ctttttaata atctactaac tacttattat atctaatttt acttgctaac
     2821 ttagctactg ttttattgtc gtagggttta atatagactg tttcaatatt ctcctctatt
     2881 gctttttttg ttatcttatt catttgacta taatcactat tttgtgaatt ttcccattta
     2941 taagggatat tattatatac attaattgtt ccatcaccat tactgctata tgttacagac
     3001 ccaccagctc tcataggagc ttctaatttc acaacttttt ctttataaat tgcactattt
     3061 ttggcttgtg gatttacttt tgagcctttt ggtatttctg taacataaac atttttaata
     3121 ccttttaaat catttcttgt agatattaac tgataccaaa ctcgtgcata ttcaatttct
     3181 ttcgagttaa aattatttaa gtaactttta tttttatcag tagattgttg cttcttatta
     3241 ctatcattat ttttttcttg ttttttatta tcattttttt ctttattttg tttattacta
     3301 ctttctgaat tatccgcact atcagatgtg ttagtttttt cgtcaatttc atttgatgct
     3361 tcattatctt tgttttctga ggtgttagta tcactagaat tattatcatt gtgatgttct
     3421 gaatcgttat tattacaact cactaaaatt accgataaac taacaattaa tataggtaaa
     3481 atgatttttt tcatattaca actccttttc agtatttcaa attagattat gttaatattt
     3541 tttcagcaag aaaacgtaaa ttacatgagt tgaaaaatat gtattaaata tataataaaa
     3601 taaccacatg ttttacataa acacggtggt tattttatca atttttatat tgcctttttt
     3661 ctgaatatta aaagacttat acctaatatt agtagtccag aagcgccaat tattgacaat
     3721 atatcacttt ggttgctttc tcctgtttct ggtaatactt gagagtttga atcttgattt
     3781 ttcattcctt cagaactctt gtttttattt tcagttacag gtgtcaacac accttgaggt
     3841 gtattaatag ttttattttc ttcattagct atcttttgac tttcattttt tacaatttga
     3901 ttatattttt ctgtggatac actattaggg ttaggaacat gactttctaa ttcttggtat
     3961 tcttcttgag tcatacaatt aggtatcacc cctccgaaag gtcttttacc ttgttgagtt
     4021 ctctcattat tttgtatatc agtactttgt gattgatatt cattaaatac acttgaaact
     4081 ttctcattat actcgatttg agttaattcc cctgaatctt tctgtttaga aagttcactt
     4141 atttgatttt gtgcagattg tgggagggat gaagtattga agtctacatt agcccaacca
     4201 tctccatttg tatctgcgct atttggtaag ttctctccgt atacggtttt ggcaccatcc
     4261 tcagcattgg ctgtcgctga aaaacctgtg aatgttaatg agctagccaa taataaagtt
     4321 ggaagtaatg atcgtttttt cataaaatta tctcctttaa tagaatttgt attttcatta
     4381 tcacatagat acattaaaca taaaatgact tatcggaagt gcatatgctt aacaattcac
     4441 aaatgatata atataaaagt cgtcgttcta cttctgtgct ggtttagcat ggaaggaggt
     4501 gacaccatgt tcagcatcat atttgtatct atagtagctc ctattatagt aggggttata
     4561 atcacgttgt tttcacattg gttgaataat cgtgataaac aataaagcga cgacacagta
     4621 ataacaccaa aaagagcccg ataactactg gaatagttat cgggctcggt gtttaccatg
     4681 ttcaacacat atttgttatc taaattataa catcatagtg tggaggaatg caaaattgaa
     4741 taattagtat gttaagtcta tactatactg attagtaagt actaattatc atctttgtta
     4801 ttgttacccc tttcattggc ttttgaatct aattcttttt gaaatttttt gtgtgatttt
     4861 gaagaagtgt ttaaaaagaa ataggtcata ttatccatac gatgtccttt tataaattca
     4921 ttgataaatt gtttttttgt catttttttg gttgtattac gttgtttttg caatctaatg
     4981 atttcttctt caatgtatac tcttatcatc aaatctaaac tagatgtctg attataagta
     5041 gtgaataatt ttggaactaa taaagtttta ttaccatcaa aaagaaatgg agtgaaatca
     5101 taagtttccc atataccgtt agtagagaaa taataatatg tcacagtttc tttagaaaga
     5161 aacgatttca tttctggaaa tgactgaatc atggatttat taaaatcaat tataacattc
     5221 atgattatat tacttgttaa atctgataat ttatcatcac caaaatttct gattaatggt
     5281 gctttttgag gtattgttaa tccgtcatca tttaagataa tggaaagaat atcttcattc
     5341 ataaatagat taatcaattc agatgctgta gtaccttttc ctgtattacc tttctgagaa
     5401 aaacctaagc gtaaatggtg tggttctgaa aaatgagaga ataattttct aaattcatta
     5461 cgcttttggg tatcagaaaa atcactaaga tgtatatcga taattttttc aaaaaaaaca
     5521 tcaatttgct cttgtgctct ttgagccaaa gtcgcaactt ttccaacttc tctattagat
     5581 attactttaa taaggtttgg gtctatatat tgaagagtgt cttcttcaat agaaatattt
     5641 aaataatcag ttgatttagc atcatgattg aatttggtac atactagtgt cactctatat
     5701 catccttctg taagtttttt gtttacttcc aatatagaat gatgtattat tgtaattgtc
     5761 gatttacaat aagttgttat tctaggtgga agtttattgc ttttgatgta tagatagact
     5821 taaccttttc attatagcat actatagaag aaaatgcaag caaaagcata taaacaacaa
     5881 atgttgttta tatgcttttg cttttttgtt aattggagag gacaaggtga atattaacag
     5941 tcaaaatcca gttatgtcgg ttaatgaatc gatgaaaatt ttcaaagctc aagtaatgct
     6001 tagaaattta aagctattaa gcgagtggat tgttaaaggg gactgaagtt aaagacactt
     6061 tttaagaaga tttttcaatt aaagggtata ttaaaacttt aatttagtac tttatatttt
     6121 tcttgactct ttaataatcc tatatacagt agaacgactc actcctgttt gttcagcaat
     6181 ttcttcacca gacagctttt gttcatcata taagaatttg atctctcttt tcttatgatc
     6241 tggtagtgaa ggtcttccac cttttctccc tcttgctctt gcagcttcca aacctttttt
     6301 agttcgttca cttaataaat tagcttcaag ttcagcgaat gcactcatca tggtaaagaa
     6361 catcttgccc atcgcatctt tcgttgacac attcatatct ataatatgta aatcaatgct
     6421 attttcatcc aaccattgag atagctcaat taattgtttt gttgtacgtc caagacgatc
     6481 taatttatag acaactagaa tatctccttc acgtaaataa tctaaacact tatcaagttc
     6541 cgttctttta gttttacgac cactcgcttt ctcgctaaag atacgttcac aaccatattc
     6601 ctcaagtgta tcaatctgtc catcaagact ttgatcttgt gttgacacac gtgcataacc
     6661 aattttagcc atttgaacac cacctttttt aatcgtttta tgggtactta ctaatgaaat
     6721 atagttctcg atgtaaataa acacgtctgt cagcatctaa aaactcatga ttttcgatat
     6781 ttatgataca ttgattataa cacaagttta tagacttaaa tatccaagaa aaacctctta
     6841 atattaagtt aagaggtttt gtgtcaaaaa cgaacgtaat ttagaaataa tatttattgg
     6901 tatttcaaga atatatatat aattatttac taagtccatt taaaaaaatt tgagagaact
     6961 tgttcataag tttaattctt tcattaatat tttgttcatg tgtaaaggta acaattccat
     7021 taacagcgtt tgctgctatt ttactaacag cattaacatc attaatacac cattcgccat
     7081 ttaaattacc ctccttaaaa attacatgat aagcatctat atatttgttt tctaatttat
     7141 tcattttttc attaatgcta ttagttttat aatattcagt gtaaaattca attattgcgt
     7201 tttgaagtgg ataatagtat tcggttgtta aagatagttc attatataaa taaaattttt
     7261 ctctattagt tttacatttg atttgttcct ttttccactg ttcttgccat ttagattctt
     7321 ctatatttaa aatttctaaa aatagatttt cttttgtttt aaagtgataa taaagattcc
     7381 ctttactact ttctgataat ttaacaattt ctccagtagt agtggcatta tatccatttt
     7441 ttataaataa ttcctttgcg acacctagta ttttatcttt caagttcatc acgcctttaa
     7501 tgagtattaa gtactatcaa tgatagcaca aaaaattcaa agaataattg tataacacaa
     7561 tacagccatt taaatttcgc aagatttttt gttgtaatat gtaaaaaaaa tagattataa
     7621 tccttataga ccgatcgcac ggtctataag gattggaggg aacttaaatg atttcatttt
     7681 ttacaaaaac tactgatatg atgacatcaa aaaaaagatg gactgcacta gtagtattag
     7741 ctgttagttt gtttgttgtt acaatggata tgacaatatt aattatggct ttaccggaat
     7801 tagtaagaga gttagagcct tctggtaccc aacagttatg gatagttgat atatactctc
     7861 ttgttttagc tggctttata attccattga gtgcctttgc tgataaatgg ggaagaaaaa
     7921 aagcattatt aactggattt gctttatttg gcctcgtttc attagctata tttttcgcag
     7981 aaagtgcaga gttcgtaata gctattcgat ttttacttgg tattgcaggt gctttaataa
     8041 tgccaactac tctttcaatg ataagagtaa tttttgaaaa ccctaaagaa agggccactg
     8101 cattagctgt atggtcaatc gcttcatcga taggtgctgt ttttggacca attatcggag
     8161 gagctttact tgagcaattt tcatggcact cggcattttt aattaatgta ccgtttgcga
     8221 taatagcagt tgtagcaggt ttatttttat taccagagtc taagttatca aaagaaaagt
     8281 ctcactcgtg ggatattcct tctacaattt tatcaattgc aggcatgatt ggactggtat
     8341 ggagtatcaa agaattttca aaagaaggac tagcagatat tattccatgg gttgtaatag
     8401 tattagcaat taccatgata gtgatatttg ttaaacgtaa tttatcaagt tctgatccaa
     8461 tgttagacgt aagacttttt aaaaagagat cattttcagc tggtacaatt gctgcattta
     8521 tgacaatttt tgcaatggca tctgttttgt tattagcttc acaatggtta caggttgtgg
     8581 aagaactttc tccttttaaa gctggcttat acctattacc tatggcaata ggagatatgg
     8641 tgtttgcacc aattgcaccc ggattagcgg cgcgatttgg accgaaaata gtgttacctt
     8701 ccggaattgg aattgcagcc attggcatgt ttattatgta tttctttggt catccattat
     8761 catattctac aatggcttta gcattaattt tagttggagc tggtatggct tcactagcag
     8821 ttgcatctgc tctaataatg ttagaaacac ctacatcaaa agcaggtaat gcagctgctg
     8881 ttgaagagtc tatgtatgac cttggaaatg tttttggtgt agcagtactt ggtagcctat
     8941 cttctatgct ttatcgtgta tttttagata tttcatcttt ttcatcaaaa ggtatagttg
     9001 gagatttagc tcatgtagct gaagaatctg tagtgggcgc tgtcgaagta gctaaagcta
     9061 cggggataaa acagcttgca aacgaggctg taacatcatt taatgatgct tttgtagcaa
     9121 ctgctttagt aggtgggatt atcatgatta tcatttcaat agttgtctat ttgttaattc
     9181 ccaaatcact tgatataact aaacaaaaat agtactaaac atatattttt agatattgta
     9241 gattagttta tttttaaaat tagtaatagt taaaaagtag aaccattaat tttaaatggt
     9301 tctacttttt tattattaag taaaattata aaagacaatt tcttaaatta ataatatgat
     9361 atacctatat ctaataattt attagtatta ataaaccttg aaaggatgat taactgatga
     9421 atagagcaga tatttttaaa atatttgatg tagaacttaa tataactata tttttaggag
     9481 cactagtagc aactttcatc tattttataa gttacatgaa caatgtaaat gaattaatta
     9541 cattatgctt aacattagct tttcctgcat atttattaaa aattagcaga tcgttcgagg
     9601 ttattttatt agaggaaaaa ataaaaaata gtaaaatcaa aatctgtgac aaagatagaa
     9661 ttgaacgaga gatactggtg aaaaagaacc taatagatga actattgttt cctaatagag
     9721 ttattaaaaa atatacactg acaattttaa tttacagtat aggtattatt attcttttac
     9781 tactttgtaa tatgttaagt tatggaattg gtgaaaatgt caattttatc tggagattac
     9841 tagcttgtta tgtaacgagt gtcacagtga ttatagttgc cttgattata atgataatga
     9901 tatttgatct tataattgga ttactattaa attttcaaat atttttaaaa aataaaaagt
     9961 atgatgattt tttataatta ttcgatttat ataatgaaaa attaaatgat taaacataga
    10021 aacctcaaaa caaaccatat ttaatagagt ttatggcgcc ttatacaaat aaaggctatg
    10081 ctgttaaaga attatgtagc tatcttcaga ttaatatgga tgatgtttat actattggcg
    10141 atagaaaatt gatttaccta tgtttaatac cctagataat tcttctgata atttagtttt
    10201 tgttagagtt taacataact gatgattatc agaagtttga ttcacattaa aaaggcacaa
    10261 caagttcatg aacttgtgtg cctttttaat tgcaattagt taccaaataa acgactccaa
    10321 aatctctttt taggtggttc tacctcccta ttatcactgg aatcgttctt cgttttttca
    10381 acaaacttat cctcttttgt taattcatct gtactgtcat gcttattctc ttcagttata
    10441 tttttatgat ttacttcggt tggttgacat tctttttgtt gttggttgat attttgagat
    10501 tcttcagtaa aagttgcttc ttgtggatta atattctgtc tattattcat cgttgtatca
    10561 aaagaatatt taagctgtct ttcttcttct aattggcttt ctagtttttg aattttttta
    10621 ttgttttcta aagataatat ttgttgattc tccaacagtt tatttaatgt attaacattg
    10681 ctattttgtt gatctaattg tttagttaga ttctcaatat atttttcatc atttttagct
    10741 ctagtttcaa agatttctat ttgcttttct aattcgttta cttttgtttt taatgtctca
    10801 gaactatttg aattattatg tatatacttt tcaacttttg cagtattttc attaaataat
    10861 tcatttttta ctgattcttt attatgagtt gttttctctt tcttagtgat tttttctata
    10921 atttttaaat aatcattatc tttaatataa ttaattccat tttctttttc aaaagatata
    10981 tttaatcttt tagcattatt aacaatagtt tgtttagtta cacctaattc atcagcaatc
    11041 attttaatag ttttcataac atcccccctt aggtacctaa tttagtacct aaattttacc
    11101 acctaacaaa atttatttca tcatttagta ctaaatttac tatctattaa ctacctaaat
    11161 gcctaattat ctatccttaa aatattttcg ccacttaatt aggtgctaaa atagcgaatt
    11221 atacgtttgg tagttttagg tgtactttta attacattta aaactcttta tatacgccat
    11281 taaaagtgtt aatattactt tataaatatt aaaagagtcg atgctattgg cgtagcatcg
    11341 actctcggta ataaaacgat tcgcactcgt ttgtttatat atttttttga tacttgtatt
    11401 atatatatct aatcatctaa gtgcaagcac aaaacatata acttacgtaa aaattgtttt
    11461 attacctcaa tcccaaaatg gaaatgaggt ttttattatg cccaattttg aaaaatataa
    11521 tttatcacaa gtaaaaactg aaagatttta tcaactgcct aaatatttat ttgaagatgc
    11581 atattttaag aaaatgtctg cagaagccaa aattatgtat gcgttattaa aagatcgttt
    11641 tgaattatcc ctccaaaatg aatgggtaga taaaaataat aatatttact ttattttcag
    11701 taataaacat ttgtgtgaat acttaggtta tgcagaacaa aaaattataa aattaaaaaa
    11761 agagttaata aaatttaatt tactaactca agaacgtgtt ggccttaata aaccaaatag
    11821 attataccta ttaaaaccta attatgacat tgaagccagt catatcaagg aacttccaaa
    11881 ttcacagttc cagaacaatg aatttggaag ttctagaact gtgaatttaa gtggtcaaga
    11941 acttccaaat tcacagtcta atgatactga ttataatgac actgattata ttaagactaa
    12001 ttataatgat atgtatgatt tgaatgatag gaaattaaca tattctagta atcatacaaa
    12061 tcattcaaat cactactatt caaaattcaa tgatgaagct ttaaaatttc aattacttga
    12121 agaactacca caaagtattc aaagttattt aagtaacttt tctgtatctg aaattaaact
    12181 tattaaatct gtgttattaa aagccaaaac atccttcaac aattctattg atgcatatta
    12241 tttattagaa gatatggaat ttgaaattgt taatgttctt aagcgtttta aagctacatt
    12301 aattcaaaaa aatgaaaccg ttgaagcaat gcaagggtac ttaatgaaat ctcttaaatc
    12361 tgaatttgca gaagtacata cgcttaataa acgacgtgat catttaccaa tcacttcttt
    12421 atttaatcaa taatacaact taataagcca acaatgatcc aattagagaa tatctttaat
    12481 tagatgttct cttaactgta agcgaacttt attttcaatg aaatgtaatt aattaagtaa
    12541 gtgggactaa aacttttctt ttttagtccc atttttataa acaattatta ttcattgtta
    12601 ctactatcag caattttttc tttttagtga aacccaagta tttgtaagca tgataaatta
    12661 cttcttttta tatttattgg gtttatttgt attaatgtag ttggaataca tctttaacaa
    12721 ttcttctgtt tcatcaggac ttaaatgctc acattcaaaa tacttttcaa gcattgcacc
    12781 ttttcgaatc agtctatgtg ctctttcttt tctatctaaa ttttttaatc tatgctttct
    12841 taattctgct ttttcaattt cagcatttat ttttttgaga tgtttaaaat cattcatcgt
    12901 atcacttact attctagtta attttgtctt ctgttaaatt ttccttgttc tcgttgctgc
    12961 tattttgttc gatatcttta ttaaattctc caatgacttt atccataatt ttatacaatt
    13021 catcactaga gttgatcata tttaaatcaa gtttatttaa aaaataacca ccaattttat
    13081 tagatagttg ttcttcttgt tttttctttt gctctttcag ttgtgcaatt tgtttttcta
    13141 attgacttaa ttttgtaata tctgtttgtt taattttaga cattaaaaaa ctcccttagt
    13201 ttatctatat aaagcatacc ataattcatt gggtaagtcg cacatccatg gtataatata
    13261 accagttctt aagacactgc actgcccatt tacatgcaaa ttgaaaattg gcataaaaat
    13321 gggcaaccat actggttgat cattatagtt cccacagggg taaaaaaaca gaaaagagag
    13381 gaggtaaaat catgaataaa ggttacttta tgttgagagt gactaacatt aatcgagcaa
    13441 caaaaaatat cgttgcgtca gcgtcttatc gttctgatga agcattatat agcgaacgta
    13501 cagatgaaaa aattaagttt agaaatcata ctattaagcc tgaaagcatg attttaactc
    13561 ctcaacatgc
//
