LOCUS       NZ_CP073853            13570 bp    DNA     circular CON 16-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1266915 plasmid p1B1266915,
            complete sequence.
ACCESSION   NZ_CP073853
VERSION     NZ_CP073853.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699882
            Assembly: GCF_019329625.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 442x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019329625.1-RS_2024_04_16
            Annotation Date                   :: 04/16/2024 07:53:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,601
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,454
            CDSs (with protein)               :: 2,454
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 36 of 66
            Pseudo Genes (incomplete)         :: 37 of 66
            Pseudo Genes (internal stop)      :: 23 of 66
            Pseudo Genes (multiple problems)  :: 21 of 66
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073853.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1266915"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p1B1266915"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(12229..13570,1..203))
                     /gene="qac"
                     /locus_tag="KFV26_RS12810"
                     /old_locus_tag="KFV26_12820"
     CDS             complement(join(12229..13570,1..203))
                     /gene="qac"
                     /locus_tag="KFV26_RS12810"
                     /old_locus_tag="KFV26_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            382..948
                     /gene="qacR"
                     /locus_tag="KFV26_RS12815"
                     /old_locus_tag="KFV26_12825"
     ARG             383..945
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1199..1759
                     /locus_tag="KFV26_RS12820"
                     /old_locus_tag="KFV26_12830"
     CDS             1199..1759
                     /locus_tag="KFV26_RS12820"
                     /old_locus_tag="KFV26_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            2178..3089
                     /locus_tag="KFV26_RS12825"
                     /old_locus_tag="KFV26_12835"
     CDS             2178..3089
                     /locus_tag="KFV26_RS12825"
                     /old_locus_tag="KFV26_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(3266..3364)
                     /locus_tag="KFV26_RS12830"
                     /old_locus_tag="KFV26_12840"
     CDS             complement(3266..3364)
                     /locus_tag="KFV26_RS12830"
                     /old_locus_tag="KFV26_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            3528..4226
                     /locus_tag="KFV26_RS12835"
                     /old_locus_tag="KFV26_12845"
     CDS             3528..4226
                     /locus_tag="KFV26_RS12835"
                     /old_locus_tag="KFV26_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            4377..5081
                     /locus_tag="KFV26_RS12840"
                     /old_locus_tag="KFV26_12850"
     CDS             4377..5081
                     /locus_tag="KFV26_RS12840"
                     /old_locus_tag="KFV26_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(5130..5363)
                     /locus_tag="KFV26_RS12845"
                     /old_locus_tag="KFV26_12855"
     CDS             complement(5130..5363)
                     /locus_tag="KFV26_RS12845"
                     /old_locus_tag="KFV26_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(5440..5853)
                     /locus_tag="KFV26_RS13115"
                     /old_locus_tag="KFV26_12860"
     CDS             complement(5440..5853)
                     /locus_tag="KFV26_RS13115"
                     /old_locus_tag="KFV26_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            complement(6127..8049)
                     /locus_tag="KFV26_RS12855"
                     /old_locus_tag="KFV26_12865"
     CDS             complement(6127..8049)
                     /locus_tag="KFV26_RS12855"
                     /old_locus_tag="KFV26_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            8258..8527
                     /locus_tag="KFV26_RS12860"
                     /old_locus_tag="KFV26_12870"
     CDS             8258..8527
                     /locus_tag="KFV26_RS12860"
                     /old_locus_tag="KFV26_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            8544..8783
                     /locus_tag="KFV26_RS12865"
                     /old_locus_tag="KFV26_12875"
     CDS             8544..8783
                     /locus_tag="KFV26_RS12865"
                     /old_locus_tag="KFV26_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(9008..9943)
                     /locus_tag="KFV26_RS12870"
                     /old_locus_tag="KFV26_12880"
     CDS             complement(9008..9943)
                     /locus_tag="KFV26_RS12870"
                     /old_locus_tag="KFV26_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            10384..11145
                     /locus_tag="KFV26_RS12875"
                     /old_locus_tag="KFV26_12885"
     CDS             10384..11145
                     /locus_tag="KFV26_RS12875"
                     /old_locus_tag="KFV26_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            complement(11464..12024)
                     /locus_tag="KFV26_RS12885"
                     /old_locus_tag="KFV26_12895"
     CDS             complement(11464..12024)
                     /locus_tag="KFV26_RS12885"
                     /old_locus_tag="KFV26_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     ARG             complement(4..203)
                     /name="qacB"
                     /gene="qacB"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic"
                     /identity="98.51"
                     /coverage="13.04"
     ARG             complement(12230..13569)
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic"
                     /identity="99.78"
                     /coverage="86.77"
     Typing          complement(9009..9943)
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6197..8049)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 gctaaaacaa gagagtatat atcaactatc cataactgtt gggtaccaga aggctctaac
       61 tctcttacta attccggtaa agccataatt aatattgtca tatccattgt aacaacaaac
      121 aaactaacag ctaatactac tagtgcagtc catctttttt ttgatgtcat catatcagta
      181 gtttttgtaa aaaatgaaat catttaagtt ccctccaatc cttatagacc gtgcgatcgg
      241 tctataagga ttataatcta ttttttttac atattacaac aaaaaatctt gcgaaattta
      301 aatggctgta ttgtgttata caattattct ttgaattttt tgtgctatca ttgatagtac
      361 ttaatactca ttaaaggcgt gatgaacttg aaagataaaa tactaggtgt cgcaaaggaa
      421 ttatttataa aaaatggata taatgccact actactggag aaattgttaa attatcagaa
      481 agtagtaaag ggaatcttta ttatcacttt aaaacaaaag aaaatctatt tttagaaatt
      541 ttaaatatag aagaatctaa atggcaagaa cagtggaaaa aggaacaaat caaatgtaaa
      601 actaatagag aaaaatttta tttatataat gaactatctt taacaaccga atactattat
      661 ccacttcaaa acgcaataat tgaattttac actgaatatt ataaaactaa tagcattaat
      721 gaaaaaatga ataaattaga aaacaaatat atagatgctt atcatgtaat ttttaaggag
      781 ggtaatttaa atggcgaatg gtgtattaat gatgttaatg ctgttagtaa aatagcagca
      841 aacgctgtta atggaattgt tacctttaca catgaacaaa atattaatga aagaattaaa
      901 cttatgaaca agttctctca aattttttta aatggactta gtaaataatt atatatatat
      961 tcttgaaata ccaataaata ttatttctaa attacgttcg tttttgacac aaaacctctt
     1021 aacttaatat taagaggttt ttcttggata tttaagtcta taaacttgtg ttataatcaa
     1081 tgtatcataa atatcgaaaa tcatgagttt ttagatgctg acagacgtgt ttatttacat
     1141 cgagaactat atttcattag taagtaccca taaaacgatt aaaaaaggtg gtgttcaaat
     1201 ggctaaaatt ggttatgcac gtgtgtcaac acaagatcaa agtcttgatg gacagattga
     1261 tacacttgag gaatatggtt gtgaacgtat ctttagcgag aaagcgagtg gtcgtaaaac
     1321 taaaagaacg gaacttgata agtgtttaga ttatttacgt gaaggagata ttctagttgt
     1381 ctataaatta gatcgtcttg gacgtacaac aaaacaatta attgagctat ctcaatggtt
     1441 ggatgaaaat agcattgatt tacatattat agatatgaat gtgtcaacga aagatgcgat
     1501 gggcaagatg ttctttacca tgatgagtgc attcgctgaa cttgaagcta atttattaag
     1561 tgaacgaact aaaaaaggtt tggaagctgc aagagcaaga gggagaaaag gtggaagacc
     1621 ttcactacca gatcataaga aaagagagat caaattctta tatgatgaac aaaagctgtc
     1681 tggtgaagaa attgctgaac aaacaggagt gagtcgttct actgtatata ggattattaa
     1741 agagtcaaga aaaatataaa gtactaaatt aaagttttaa tatacccttt aattgaaaaa
     1801 tcttcttaaa aagtgtcttt aacttcagtc ccctttaaca atccactcgc ttaatagctt
     1861 taaatttcta agcattactt gagctttgaa aattttcatc gattcattaa ccgacataac
     1921 tggattttga ctgttaatat tcaccttgtc ctctccaatt aacaaaaaag caaaagcata
     1981 taaacaacat ttgttgttta tatgcttttg cttgcatttt cttctatagt atgctataat
     2041 gaaaaggtta agtctatcta tacatcaaaa gcaataaact tccacctaga ataacaactt
     2101 attgtaaatc gacaattaca ataatacatc attctatatt ggaagtaaac aaaaaactta
     2161 cagaaggatg atatagagtg acactagtat gtaccaaatt caatcatgat gctaaatcaa
     2221 ctgattattt aaatatttct attgaagaag acactcttca atatatagac ccaaacctta
     2281 ttaaagtaat atctaataga gaagttggaa aagttgcgac tttggctcaa agagcacaag
     2341 agcaaattga tgtttttttt gaaaaaatta tcgatataca tcttagtgat ttttctgata
     2401 cccaaaagcg taatgaattt agaaaattat tctctcattt ttcagaacca caccatttac
     2461 gcttaggttt ttctcagaaa ggtaatacag gaaaaggtac tacagcatct gaattgatta
     2521 atctatttat gaatgaagat attctttcca ttatcttaaa tgatgacgga ttaacaatac
     2581 ctcaaaaagc accattaatc agaaattttg gtgatgataa attatcagat ttaacaagta
     2641 atataatcat gaatgttata attgatttta ataaatccat gattcagtca tttccagaaa
     2701 tgaaatcgtt tctttctaaa gaaactgtga catattatta tttctctact aacggtatat
     2761 gggaaactta tgatttcact ccatttcttt ttgatggtaa taaaacttta ttagttccaa
     2821 aattattcac tacttataat cagacatcta gtttagattt gatgataaga gtatacattg
     2881 aagaagaaat cattagattg caaaaacaac gtaatacaac caaaaaaatg acaaaaaaac
     2941 aatttatcaa tgaatttata aaaggacatc gtatggataa tatgacctat ttctttttaa
     3001 acacttcttc aaaatcacac aaaaaatttc aaaaagaatt agattcaaaa gccaatgaaa
     3061 ggggtaacaa taacaaagat gataattagt acttactaat cagtatagta tagacttaac
     3121 atactaatta ttcaattttg cattcctcca cactatgatg ttataattta gataacaaat
     3181 atgtgttgaa catggtaaac accgagcccg ataactattc cagtagttat cgggctcttt
     3241 ttggtgttat tactgtgtcg tcgctttatt gtttatcacg attattcaac caatgtgaaa
     3301 acaacgtgat tataacccct actataatag gagctactat agatacaaat atgatgctga
     3361 acatggtgtc acctccttcc atgctaaacc agcacagaag tagaacgacg acttttatat
     3421 tatatcattt gtgaattgtt aagcatatgc acttccgata agtcatttta tgtttaatgt
     3481 atctatgtga taatgaaaat acaaattcta ttaaaggaga taattttatg aaaaaacgat
     3541 cattacttcc aactttatta ttggctagct cattaacatt cacaggtttt tcagcgacag
     3601 ccaatgctga ggatggtgcc aaaaccgtat acggagagaa cttaccaaat agcgcagata
     3661 caaatggaga tggttgggct aatgtagact tcaatacttc atccctccca caatctgcac
     3721 aaaatcaaat aagtgaactt tctaaacaga aagattcagg ggaattaact caaatcgagt
     3781 ataatgagaa agtttcaagt gtatttaatg aatatcaatc acaaagtact gatatacaaa
     3841 ataatgagag aactcaacaa ggtaaaagac ctttcggagg ggtgatacct aattgtatga
     3901 ctcaagaaga ataccaagaa ttagaaagtc atgttcctaa ccctaatagt gtatccacag
     3961 aaaaatataa tcaaattgta aaaaatgaaa gtcaaaagat agctaatgaa gaaaataaaa
     4021 ctattaatac acctcaaggt gtgttgacac ctgtaactga aaataaaaac aagagttctg
     4081 aaggaatgaa aaatcaagat tcaaactctc aagtattacc agaaacagga gaaagcaacc
     4141 aaagtgatat attgtcaata attggcgctt ctggactact aatattaggt ataagtcttt
     4201 taatattcag aaaaaaggca atataaaaat tgataaaata accaccgtgt ttatgtaaaa
     4261 catgtggtta ttttattata tatttaatac atatttttca actcatgtaa tttacgtttt
     4321 cttgctgaaa aaatattaac ataatctaat ttgaaatact gaaaaggagt tgtaatatga
     4381 aaaaaatcat tttacctata ttaattgtta gtttatcggt aattttagtg agttgtaata
     4441 ataacgattc agaacatcac aatgataata attctagtga tactaacacc tcagaaaaca
     4501 aagataatga agcatcaaat gaaattgacg aaaaaactaa cacatctgat agtgcggata
     4561 attcagaaag tagtaataaa caaaataaag aaaaaaatga taataaaaaa caagaaaaaa
     4621 ataatgatag taataagaag caacaatcta ctgataaaaa taaaagttac ttaaataatt
     4681 ttaactcgaa agaaattgaa tatgcacgag tttggtatca gttaatatct acaagaaatg
     4741 atttaaaagg tattaaaaat gtttatgtta cagaaatacc aaaaggctca aaagtaaatc
     4801 cacaagccaa aaatagtgca atttataaag aaaaagttgt gaaattagaa gctcctatga
     4861 gagctggtgg gtctgtaaca tatagcagta atggtgatgg aacaattaat gtatataata
     4921 atatccctta taaatgggaa aattcacaaa atagtgatta tagtcaaatg aataagataa
     4981 caaaaaaagc aatagaggag aatattgaaa cagtctatat taaaccctac gacaataaaa
     5041 cagtagctaa gttagcaagt aaaattagat ataataagta gttagtagat tattaaaaag
     5101 gcgcctaaaa ttaggcgtct tttttatcat tagttcaaaa gataagcata acgttccatg
     5161 taacagcata taaagtaatg aatcaattca tcactatcta atgccaccat tgttagttca
     5221 gccttgcatg gtttaacgta tctcacataa tggtacaaat cttcaaatag tgcctgtttt
     5281 tcgggcattt taaacacctt gtgtacatta aaacctattt cttctaatcg ctttgtaatc
     5341 acgccgaaaa cgaatgtgtt catgtgaaat cactccatca tttttaatat tcacatgtac
     5401 actaaaaaaa atccgataca tcggcttttt atttttggtt taatttattt ctatattgtt
     5461 ttcactatcg ctcggacgca taagtgttaa aacaccttct accataccta tcacacccgg
     5521 tattcccgtc caacaaaata gtaaataaag aagagcattt ttatttttct taacataaaa
     5581 cttatgagcc ccgaaacctc ctagaaaaaa tgctaatgca gagtaaacat atttattaac
     5641 ttgcatgatt tccactcctt taattaattg atttagagaa acattaaata atttagtgat
     5701 ttctaaaatt gtttttaagt cgggattact tacttctctt tcccaattag aaatagaatg
     5761 tctcgatacc cctaactttt cagctaattt ttcttgagac atgtttttac tttttcttaa
     5821 cttttgtatt tgatgtccaa tttttgtttc caaattattt ttctccttaa ctttaagata
     5881 tatttaaact caattttttc tatcgatatt tatgaacatt agtatttttt atgctgaaat
     5941 atgtgaacaa tcgcataata tcaaggtttt tctataatta tacacgtttt aaaatacatt
     6001 gtgataatac atagaatgta ccttatctaa gtatataaat gttttcttta aaccaaagct
     6061 aatttaccac attgggttaa gtttcttatt attaaagaat gaactttgta tgatttcaac
     6121 tgcttactat cttctcattt ggttatttct ctcttgtttc ttcttctttt tctgatgatt
     6181 aatattgttt tgcttttcag tttcgttcgc atagtacata agaaagtcac tagcatttat
     6241 cgttggtaaa ttaatgtgat tagtttgttc attttcatgt tcaatacgat tgtcatgatc
     6301 atttctatct acttgattaa tatgtttttc gataacttct tcttgatcga catgattata
     6361 tttctcaagt ttaatgagta attgaacttt ttcattaggt gtttggtctt tgactttttc
     6421 aaagttttta tcgcctatat ctattcttaa atcatcatca atatgttctt tataactatc
     6481 aatttctttt tctttttcct taataaattg caactcaacg ttatattcaa agctatcttt
     6541 ttctaaattt ttaagatgtt tttgactaaa gaaaatatct ttactcgctt ttttataagt
     6601 atcttctagc aatacatcat taggaatatt gttattttgt ttgaacaggt attgaacatg
     6661 agttgctttt ataatattac ctttttgtgc ctcatcaaca ataaatgctt tttcatcatg
     6721 gctaaaatga tgatgagatt tagggtacat ttgctgaaca atgagatgat ttttttcact
     6781 ttcagttaga taaacatctt tagctgattg ataaatctct ttacgttttt cctctctact
     6841 ttctaattta ttcttctcat tgagtaacgc tttatagtta gtttcaacag tattaacaaa
     6901 ctcttgttga ctatatcctt tactttcaat ttcttttcta aactgataat tttcatcata
     6961 atttttagca tagaatttta attgacgtct cttaatttca tttttaagta aatcattttg
     7021 aattttacga ccaaaggtag acgcactgtc aaacgtattt ttaattgttt ctttcgcctc
     7081 tgaatagcca acatcacgtt gatatctatc aaataaaata gaataacttt gttcattttt
     7141 acttacttgt tcgttagcat tttgaataaa taatctaatt tcattttgtg cttgttcttt
     7201 ctctttatca ctaaataatt gtgatacttc gccacgatta tatcttctaa tttgttcatt
     7261 tttctgtccg taataagtaa cgggttggta ctcgataccc tctttttcac agcgttttct
     7321 ttcattttgt tctaatctat attcttctct tgtaagtgga atttcggcaa ttttttctaa
     7381 accttgcgct tcaaaagaac gactatcata tcgtcttaaa ctttgttcac gttctgagta
     7441 ttgatttaat tttaattccc aatgcttacg aacttctttg aaatcaaagt cggcaaatcg
     7501 ttcttgtttt ctaactttat taccattttt atttaaaatc gcattgccat tttcatcata
     7561 ttgcgttctc gtttttgtct ttttccccca agtaccgtct gcattaaacg gtctttgagt
     7621 aagtagaaca tgggcgtgtg ggttgtttat atcatctcta tgaattgaaa tatcagcaac
     7681 cattcctttg tctacaaact cactttttat aaaatcttta gttaattcag tttgtacctc
     7741 tctatcaaaa tcattaggca aacttaataa gacttctttt gctaatctag gattttttgt
     7801 tttcgcatta ttcttttcaa ctttatcaac ttcattccat aaacgctgtc tatctttagt
     7861 ccattcgggt gcatgttgag gagttaaaat catgctttca ggcttaatag tatgatttct
     7921 aaacttaatt ttttcatctg tacgttcgct atataatgct tcatcagaac gataagacgc
     7981 tgacgcaacg atattttttg ttgctcgatt aatgttagtc actctcaaca taaagtaacc
     8041 tttattcatg attttacctc ctctcttttc tgttttttta cccctgtggg aactataatg
     8101 atcaaccagt atggttgccc atttttatgc caattttcaa tttgcatgta aatgggcagt
     8161 gcagtgtctt aagaactggt tatattatac catggatgtg cgacttaccc aatgaattat
     8221 ggtatgcttt atatagataa actaagggag ttttttaatg tctaaaatta aacaaacaga
     8281 tattacaaaa ttaagtcaat tagaaaaaca aattgcacaa ctgaaagagc aaaagaaaaa
     8341 acaagaagaa caactatcta ataaaattgg tggttatttt ttaaataaac ttgatttaaa
     8401 tatgatcaac tctagtgatg aattgtataa aattatggat aaagtcattg gagaatttaa
     8461 taaagatatc gaacaaaata gcagcaacga gaacaaggaa aatttaacag aagacaaaat
     8521 taactagaat agtaagtgat acgatgaatg attttaaaca tctcaaaaaa ataaatgctg
     8581 aaattgaaaa agcagaatta agaaagcata gattaaaaaa tttagataga aaagaaagag
     8641 cacatagact gattcgaaaa ggtgcaatgc ttgaaaagta ttttgaatgt gagcatttaa
     8701 gtcctgatga aacagaagaa ttgttaaaga tgtattccaa ctacattaat acaaataaac
     8761 ccaataaata taaaaagaag taatttatca tgcttacaaa tacttgggtt tcactaaaaa
     8821 gaaaaaattg ctgatagtag taacaatgaa taataattgt ttataaaaat gggactaaaa
     8881 aagaaaagtt ttagtcccac ttacttaatt aattacattt cattgaaaat aaagttcgct
     8941 tacagttaag agaacatcta attaaagata ttctctaatt ggatcattgt tggcttatta
     9001 agttgtatta ttgattaaat aaagaagtga ttggtaaatg atcacgtcgt ttattaagcg
     9061 tatgtacttc tgcaaattca gatttaagag atttcattaa gtacccttgc attgcttcaa
     9121 cggtttcatt tttttgaatt aatgtagctt taaaacgctt aagaacatta acaatttcaa
     9181 attccatatc ttctaataaa taatatgcat caatagaatt gttgaaggat gttttggctt
     9241 ttaataacac agatttaata agtttaattt cagatacaga aaagttactt aaataacttt
     9301 gaatactttg tggtagttct tcaagtaatt gaaattttaa agcttcatca ttgaattttg
     9361 aatagtagtg atttgaatga tttgtatgat tactagaata tgttaatttc ctatcattca
     9421 aatcatacat atcattataa ttagtcttaa tataatcagt gtcattataa tcagtatcat
     9481 tagactgtga atttggaagt tcttgaccac ttaaattcac agttctagaa cttccaaatt
     9541 cattgttctg gaactgtgaa tttggaagtt ccttgatatg actggcttca atgtcataat
     9601 taggttttaa taggtataat ctatttggtt tattaaggcc aacacgttct tgagttagta
     9661 aattaaattt tattaactct ttttttaatt ttataatttt ttgttctgca taacctaagt
     9721 attcacacaa atgtttatta ctgaaaataa agtaaatatt attattttta tctacccatt
     9781 cattttggag ggataattca aaacgatctt ttaataacgc atacataatt ttggcttctg
     9841 cagacatttt cttaaaatat gcatcttcaa ataaatattt aggcagttga taaaatcttt
     9901 cagtttttac ttgtgataaa ttatattttt caaaattggg cataataaaa acctcatttc
     9961 cattttggga ttgaggtaat aaaacaattt ttacgtaagt tatatgtttt gtgcttgcac
    10021 ttagatgatt agatatatat aatacaagta tcaaaaaaat atataaacaa acgagtgcga
    10081 atcgttttat taccgagagt cgatgctacg ccaatagcat cgactctttt aatatttata
    10141 aagtaatatt aacactttta atggcgtata taaagagttt taaatgtaat taaaagtaca
    10201 cctaaaacta ccaaacgtat aattcgctat tttagcacct aattaagtgg cgaaaatatt
    10261 ttaaggatag ataattaggc atttaggtag ttaatagata gtaaatttag tactaaatga
    10321 tgaaataaat tttgttaggt ggtaaaattt aggtactaaa ttaggtacct aaggggggat
    10381 gttatgaaaa ctattaaaat gattgctgat gaattaggtg taactaaaca aactattgtt
    10441 aataatgcta aaagattaaa tatatctttt gaaaaagaaa atggaattaa ttatattaaa
    10501 gataatgatt atttaaaaat tatagaaaaa atcactaaga aagagaaaac aactcataat
    10561 aaagaatcag taaaaaatga attatttaat gaaaatactg caaaagttga aaagtatata
    10621 cataataatt caaatagttc tgagacatta aaaacaaaag taaacgaatt agaaaagcaa
    10681 atagaaatct ttgaaactag agctaaaaat gatgaaaaat atattgagaa tctaactaaa
    10741 caattagatc aacaaaatag caatgttaat acattaaata aactgttgga gaatcaacaa
    10801 atattatctt tagaaaacaa taaaaaaatt caaaaactag aaagccaatt agaagaagaa
    10861 agacagctta aatattcttt tgatacaacg atgaataata gacagaatat taatccacaa
    10921 gaagcaactt ttactgaaga atctcaaaat atcaaccaac aacaaaaaga atgtcaacca
    10981 accgaagtaa atcataaaaa tataactgaa gagaataagc atgacagtac agatgaatta
    11041 acaaaagagg ataagtttgt tgaaaaaacg aagaacgatt ccagtgataa tagggaggta
    11101 gaaccaccta aaaagagatt ttggagtcgt ttatttggta actaattgca attaaaaagg
    11161 cacacaagtt catgaacttg ttgtgccttt ttaatgtgaa tcaaacttct gataatcatc
    11221 agttatgtta aactctaaca aaaactaaat tatcagaaga attatctagg gtattaaaca
    11281 taggtaaatc aattttctat cgccaatagt ataaacatca tccatattaa tctgaagata
    11341 gctacataat tctttaacag catagccttt atttgtataa ggcgccataa actctattaa
    11401 atatggtttg ttttgaggtt tctatgttta atcatttaat ttttcattat ataaatcgaa
    11461 taattataaa aaatcatcat actttttatt ttttaaaaat atttgaaaat ttaatagtaa
    11521 tccaattata agatcaaata tcattatcat tataatcaag gcaactataa tcactgtgac
    11581 actcgttaca taacaagcta gtaatctcca gataaaattg acattttcac caattccata
    11641 acttaacata ttacaaagta gtaaaagaat aataatacct atactgtaaa ttaaaattgt
    11701 cagtgtatat tttttaataa ctctattagg aaacaatagt tcatctatta ggttcttttt
    11761 caccagtatc tctcgttcaa ttctatcttt gtcacagatt ttgattttac tattttttat
    11821 tttttcctct aataaaataa cctcgaacga tctgctaatt tttaataaat atgcaggaaa
    11881 agctaatgtt aagcataatg taattaattc atttacattg ttcatgtaac ttataaaata
    11941 gatgaaagtt gctactagtg ctcctaaaaa tatagttata ttaagttcta catcaaatat
    12001 tttaaaaata tctgctctat tcatcagtta atcatccttt caaggtttat taatactaat
    12061 aaattattag atataggtat atcatattat taatttaaga aattgtcttt tataatttta
    12121 cttaataata aaaaagtaga accatttaaa attaatggtt ctacttttta actattacta
    12181 attttaaaaa taaactaatc tacaatatct aaaaatatat gtttagtact atttttgttt
    12241 agttatatca agtgatttgg gaattaacaa atagacaact attgaaatga taatcatgat
    12301 aatcccacct actaaagcag ttgctacaaa agcatcatta aatgatgtta cagcctcgtt
    12361 tgcaagctgt tttatccccg tagctttagc tacttcgaca gcgcccacta cagattcttc
    12421 agctacatga gctaaatctc caactatacc ttttgatgaa aaagatgaaa tatctaaaaa
    12481 tacacgataa agcatagaag ataggctacc aagtactgct acaccaaaaa catttccaag
    12541 gtcatacata gactcttcaa cagcagctgc attacctgct tttgatgtag gtgtttctaa
    12601 cattattaga gcagatgcaa ctgctagtga agccatacca gctccaacta aaattaatgc
    12661 taaagccatt gtagaatatg ataatggatg accaaagaaa tacataataa acatgccaat
    12721 ggctgcaatt ccaattccgg aaggtaacac tattttcggt ccaaatcgcg ccgctaatcc
    12781 gggtgcaatt ggtgcaaaca ccatatctcc tattgccata ggtaataggt ataagccagc
    12841 tttaaaagga gaaagttctt ccacaacctg taaccattgt gaagctaata acaaaacaga
    12901 tgccattgca aaaattgtca taaatgcagc aattgtacca gctgaaaatg atctcttttt
    12961 aaaaagtctt acgtctaaca ttggatcaga acttgataaa ttacgtttaa caaatatcac
    13021 tatcatggta attgctaata ctattacaac ccatggaata atatctgcta gtccttcttt
    13081 tgaaaattct ttgatactcc ataccagtcc aatcatgcct gcaattgata aaattgtaga
    13141 aggaatatcc cacgagtgag acttttcttt tgataactta gactctggta ataaaaataa
    13201 acctgctaca actgctatta tcgcaaacgg tacattaatt aaaaatgccg agtgccatga
    13261 aaattgctca agtaaagctc ctccgataat tggtccaaaa acagcaccta tcgatgaagc
    13321 gattgaccat acagctaatg cagtggccct ttctttaggg ttttcaaaaa ttactcttat
    13381 cattgaaaga gtagttggca ttattaaagc acctgcaata ccaagtaaaa atcgaatagc
    13441 tattacgaac tctgcacttt ctgcgaaaaa tatagctaat gaaacgaggc caaataaagc
    13501 aaatccagtt aataatgctt tttttcttcc ccatttatca gcaaaggcac tcaatggaat
    13561 tataaagcca
//
