LOCUS       NZ_CP073849            13570 bp    DNA     circular CON 16-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1266911 plasmid p1B1266911,
            complete sequence.
ACCESSION   NZ_CP073849
VERSION     NZ_CP073849.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699881
            Assembly: GCF_019329605.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 285x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019329605.1-RS_2024_04_16
            Annotation Date                   :: 04/16/2024 07:54:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,679
            CDSs (total)                      :: 2,598
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 40 of 78
            Pseudo Genes (incomplete)         :: 44 of 78
            Pseudo Genes (internal stop)      :: 27 of 78
            Pseudo Genes (multiple problems)  :: 24 of 78
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073849.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1266911"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p1B1266911"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(13507..13570,1..206))
                     /locus_tag="KFV33_RS00005"
                     /old_locus_tag="KFV33_00005"
     CDS             complement(join(13507..13570,1..206))
                     /locus_tag="KFV33_RS00005"
                     /old_locus_tag="KFV33_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            415..2337
                     /locus_tag="KFV33_RS00010"
                     /old_locus_tag="KFV33_00010"
     CDS             415..2337
                     /locus_tag="KFV33_RS00010"
                     /old_locus_tag="KFV33_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            2611..3024
                     /locus_tag="KFV33_RS13470"
                     /old_locus_tag="KFV33_00015"
     CDS             2611..3024
                     /locus_tag="KFV33_RS13470"
                     /old_locus_tag="KFV33_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            3101..3334
                     /locus_tag="KFV33_RS00020"
                     /old_locus_tag="KFV33_00020"
     CDS             3101..3334
                     /locus_tag="KFV33_RS00020"
                     /old_locus_tag="KFV33_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(3383..4087)
                     /locus_tag="KFV33_RS00025"
                     /old_locus_tag="KFV33_00025"
     CDS             complement(3383..4087)
                     /locus_tag="KFV33_RS00025"
                     /old_locus_tag="KFV33_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(4238..4936)
                     /locus_tag="KFV33_RS00030"
                     /old_locus_tag="KFV33_00030"
     CDS             complement(4238..4936)
                     /locus_tag="KFV33_RS00030"
                     /old_locus_tag="KFV33_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            5100..5198
                     /locus_tag="KFV33_RS00035"
                     /old_locus_tag="KFV33_00035"
     CDS             5100..5198
                     /locus_tag="KFV33_RS00035"
                     /old_locus_tag="KFV33_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(5375..6286)
                     /locus_tag="KFV33_RS00040"
                     /old_locus_tag="KFV33_00040"
     CDS             complement(5375..6286)
                     /locus_tag="KFV33_RS00040"
                     /old_locus_tag="KFV33_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(6705..7265)
                     /locus_tag="KFV33_RS00045"
                     /old_locus_tag="KFV33_00045"
     CDS             complement(6705..7265)
                     /locus_tag="KFV33_RS00045"
                     /old_locus_tag="KFV33_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            complement(7516..8082)
                     /gene="qacR"
                     /locus_tag="KFV33_RS00050"
                     /old_locus_tag="KFV33_00050"
     ARG             complement(7520..8082)
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            8261..9805
                     /gene="qac"
                     /locus_tag="KFV33_RS00055"
                     /old_locus_tag="KFV33_00055"
     CDS             8261..9805
                     /gene="qac"
                     /locus_tag="KFV33_RS00055"
                     /old_locus_tag="KFV33_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            10010..10570
                     /locus_tag="KFV33_RS00060"
                     /old_locus_tag="KFV33_00060"
     CDS             10010..10570
                     /locus_tag="KFV33_RS00060"
                     /old_locus_tag="KFV33_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(10889..11650)
                     /locus_tag="KFV33_RS00070"
                     /old_locus_tag="KFV33_00070"
     CDS             complement(10889..11650)
                     /locus_tag="KFV33_RS00070"
                     /old_locus_tag="KFV33_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            12091..13026
                     /locus_tag="KFV33_RS00075"
                     /old_locus_tag="KFV33_00075"
     CDS             12091..13026
                     /locus_tag="KFV33_RS00075"
                     /old_locus_tag="KFV33_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            complement(13251..13490)
                     /locus_tag="KFV33_RS00080"
                     /old_locus_tag="KFV33_00080"
     CDS             complement(13251..13490)
                     /locus_tag="KFV33_RS00080"
                     /old_locus_tag="KFV33_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     ARG             8262..9802
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          12092..13026
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          416..2268
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 ttattaaatt ctccaatgac tttatccata attttataca attcatcact agagttgatc
       61 atatttaaat caagtttatt taaaaaataa ccaccaattt tattagatag ttgttcttct
      121 tgttttttct tttgctcttt cagttgtgca atttgttttt ctaattgact taattttgta
      181 atatctgttt gtttaatttt agacattaaa aaactccctt agtttatcta tataaagcat
      241 accataattc attgggtaag tcgcacatcc atggtataat ataaccagtt cttaagacac
      301 tgcactgccc atttacatgc aaattgaaaa ttggcataaa aatgggcaac catactggtt
      361 gatcattata gttcccacag gggtaaaaaa acagaaaaga gaggaggtaa aatcatgaat
      421 aaaggttact ttatgttgag agtgactaac attaatcgag caacaaaaaa tatcgttgcg
      481 tcagcgtctt atcgttctga tgaagcatta tatagcgaac gtacagatga aaaaattaag
      541 tttagaaatc atactattaa gcctgaaagc atgattttaa ctcctcaaca tgcacccgaa
      601 tggactaaag atagacagcg tttatggaat gaagttgata aagttgaaaa gaataatgcg
      661 aaaacaaaaa atcctagatt agcaaaagaa gtcttattaa gtttgcctaa tgattttgat
      721 agagaggtac aaactgaatt aactaaagat tttataaaaa gtgagtttgt agacaaagga
      781 atggttgctg atatttcaat tcatagagat gatataaaca acccacacgc ccatgttcta
      841 cttactcaaa gaccgtttaa tgcagacggt acttggggga aaaagacaaa aacgagaacg
      901 caatatgatg aaaatggcaa tgcgatttta aataaaaatg gtaataaagt tagaaaacaa
      961 gaacgatttg ccgactttga tttcaaagaa gttcgtaagc attgggaatt aaaattaaat
     1021 caatactcag aacgtgaaca aagtttaaga cgatatgata gtcgttcttt tgaagcgcaa
     1081 ggtttagaaa aaattgccga aattccactt acaagagaag aatatagatt agaacaaaat
     1141 gaaagaaaac gctgtgaaaa agagggtatc gagtaccaac ccgttactta ttacggacag
     1201 aaaaatgaac aaattagaag atataatcgt ggcgaagtat cacaattatt tagtgataaa
     1261 gagaaagaac aagcacaaaa tgaaattaga ttatttattc aaaatgctaa cgaacaagta
     1321 agtaaaaatg aacaaagtta ttctatttta tttgatagat atcaacgtga tgttggctat
     1381 tcagaggcga aagaaacaat taaaaatacg tttgacagtg cgtctacctt tggtcgtaaa
     1441 attcaaaatg atttacttaa aaatgaaatt aagagacgtc aattaaaatt ctatgctaaa
     1501 aattatgatg aaaattatca gtttagaaaa gaaattgaaa gtaaaggata tagtcaacaa
     1561 gagtttgtta atactgttga aactaactat aaagcgttac tcaatgagaa gaataaatta
     1621 gaaagtagag aggaaaaacg taaagagatt tatcaatcag ctaaagatgt ttatctaact
     1681 gaaagtgaaa aaaatcatct cattgttcag caaatgtacc ctaaatctca tcatcatttt
     1741 agccatgatg aaaaagcatt tattgttgat gaggcacaaa aaggtaatat tataaaagca
     1801 actcatgttc aatacctgtt caaacaaaat aacaatattc ctaatgatgt attgctagaa
     1861 gatacttata aaaaagcgag taaagatatt ttctttagtc aaaaacatct taaaaattta
     1921 gaaaaagata gctttgaata taacgttgag ttgcaattta ttaaggaaaa agaaaaagaa
     1981 attgatagtt ataaagaaca tattgatgat gatttaagaa tagatatagg cgataaaaac
     2041 tttgaaaaag tcaaagacca aacacctaat gaaaaagttc aattactcat taaacttgag
     2101 aaatataatc atgtcgatca agaagaagtt atcgaaaaac atattaatca agtagataga
     2161 aatgatcatg acaatcgtat tgaacatgaa aatgaacaaa ctaatcacat taatttacca
     2221 acgataaatg ctagtgactt tcttatgtac tatgcgaacg aaactgaaaa gcaaaacaat
     2281 attaatcatc agaaaaagaa gaagaaacaa gagagaaata accaaatgag aagatagtaa
     2341 gcagttgaaa tcatacaaag ttcattcttt aataataaga aacttaaccc aatgtggtaa
     2401 attagctttg gtttaaagaa aacatttata tacttagata aggtacattc tatgtattat
     2461 cacaatgtat tttaaaacgt gtataattat agaaaaacct tgatattatg cgattgttca
     2521 catatttcag cataaaaaat actaatgttc ataaatatcg atagaaaaaa ttgagtttaa
     2581 atatatctta aagttaagga gaaaaataat ttggaaacaa aaattggaca tcaaatacaa
     2641 aagttaagaa aaagtaaaaa catgtctcaa gaaaaattag ctgaaaagtt aggggtatcg
     2701 agacattcta tttctaattg ggaaagagaa gtaagtaatc ccgacttaaa aacaatttta
     2761 gaaatcacta aattatttaa tgtttctcta aatcaattaa ttaaaggagt ggaaatcatg
     2821 caagttaata aatatgttta ctctgcatta gcattttttc taggaggttt cggggctcat
     2881 aagttttatg ttaagaaaaa taaaaatgct cttctttatt tactattttg ttggacggga
     2941 ataccgggtg tgataggtat ggtagaaggt gttttaacac ttatgcgtcc gagcgatagt
     3001 gaaaacaata tagaaataaa ttaaaccaaa aataaaaagc cgatgtatcg gatttttttt
     3061 agtgtacatg tgaatattaa aaatgatgga gtgatttcac atgaacacat tcgttttcgg
     3121 cgtgattaca aagcgattag aagaaatagg ttttaatgta cacaaggtgt ttaaaatgcc
     3181 cgaaaaacag gcactatttg aagatttgta ccattatgtg agatacgtta aaccatgcaa
     3241 ggctgaacta acaatggtgg cattagatag tgatgaattg attcattact ttatatgctg
     3301 ttacatggaa cgttatgctt atcttttgaa ctaatgataa aaaagacgcc taattttagg
     3361 cgccttttta ataatctact aactacttat tatatctaat tttacttgct aacttagcta
     3421 ctgttttatt gtcgtagggt ttaatataga ctgtttcaat attctcctct attgcttttt
     3481 ttgttatctt attcatttga ctataatcac tattttgtga attttcccat ttataaggga
     3541 tattattata tacattaatt gttccatcac cattactgct atatgttaca gacccaccag
     3601 ctctcatagg agcttctaat ttcacaactt tttctttata aattgcacta tttttggctt
     3661 gtggatttac ttttgagcct tttggtattt ctgtaacata aacattttta atacctttta
     3721 aatcatttct tgtagatatt aactgatacc aaactcgtgc atattcaatt tctttcgagt
     3781 taaaattatt taagtaactt ttatttttat cagtagattg ttgcttctta ttactatcat
     3841 tatttttttc ttgtttttta ttatcatttt tttctttatt ttgtttatta ctactttctg
     3901 aattatccgc actatcagat gtgttagttt tttcgtcaat ttcatttgat gcttcattat
     3961 ctttgttttc tgaggtgtta gtatcactag aattattatc attgtgatgt tctgaatcgt
     4021 tattattaca actcactaaa attaccgata aactaacaat taatataggt aaaatgattt
     4081 ttttcatatt acaactcctt ttcagtattt caaattagat tatgttaata ttttttcagc
     4141 aagaaaacgt aaattacatg agttgaaaaa tatgtattaa atatataata aaataaccac
     4201 atgttttaca taaacacggt ggttatttta tcaattttta tattgccttt tttctgaata
     4261 ttaaaagact tatacctaat attagtagtc cagaagcgcc aattattgac aatatatcac
     4321 tttggttgct ttctcctgtt tctggtaata cttgagagtt tgaatcttga tttttcattc
     4381 cttcagaact cttgttttta ttttcagtta caggtgtcaa cacaccttga ggtgtattaa
     4441 tagttttatt ttcttcatta gctatctttt gactttcatt ttttacaatt tgattatatt
     4501 tttctgtgga tacactatta gggttaggaa catgactttc taattcttgg tattcttctt
     4561 gagtcataca attaggtatc acccctccga aaggtctttt accttgttga gttctctcat
     4621 tattttgtat atcagtactt tgtgattgat attcattaaa tacacttgaa actttctcat
     4681 tatactcgat ttgagttaat tcccctgaat ctttctgttt agaaagttca cttatttgat
     4741 tttgtgcaga ttgtgggagg gatgaagtat tgaagtctac attagcccaa ccatctccat
     4801 ttgtatctgc gctatttggt aagttctctc cgtatacggt tttggcacca tcctcagcat
     4861 tggctgtcgc tgaaaaacct gtgaatgtta atgagctagc caataataaa gttggaagta
     4921 atgatcgttt tttcataaaa ttatctcctt taatagaatt tgtattttca ttatcacata
     4981 gatacattaa acataaaatg acttatcgga agtgcatatg cttaacaatt cacaaatgat
     5041 ataatataaa agtcgtcgtt ctacttctgt gctggtttag catggaagga ggtgacacca
     5101 tgttcagcat catatttgta tctatagtag ctcctattat agtaggggtt ataatcacgt
     5161 tgttttcaca ttggttgaat aatcgtgata aacaataaag cgacgacaca gtaataacac
     5221 caaaaagagc ccgataacta ctggaatagt tatcgggctc ggtgtttacc atgttcaaca
     5281 catatttgtt atctaaatta taacatcata gtgtggagga atgcaaaatt gaataattag
     5341 tatgttaagt ctatactata ctgattagta agtactaatt atcatctttg ttattgttac
     5401 ccctttcatt ggcttttgaa tctaattctt tttgaaattt tttgtgtgat tttgaagaag
     5461 tgtttaaaaa gaaataggtc atattatcca tacgatgtcc ttttataaat tcattgataa
     5521 attgtttttt tgtcattttt ttggttgtat tacgttgttt ttgcaatcta atgatttctt
     5581 cttcaatgta tactcttatc atcaaatcta aactagatgt ctgattataa gtagtgaata
     5641 attttggaac taataaagtt ttattaccat caaaaagaaa tggagtgaaa tcataagttt
     5701 cccatatacc gttagtagag aaataataat atgtcacagt ttctttagaa agaaacgatt
     5761 tcatttctgg aaatgactga atcatggatt tattaaaatc aattataaca ttcatgatta
     5821 tattacttgt taaatctgat aatttatcat caccaaaatt tctgattaat ggtgcttttt
     5881 gaggtattgt taatccgtca tcatttaaga taatggaaag aatatcttca ttcataaata
     5941 gattaatcaa ttcagatgct gtagtacctt ttcctgtatt acctttctga gaaaaaccta
     6001 agcgtaaatg gtgtggttct gaaaaatgag agaataattt tctaaattca ttacgctttt
     6061 gggtatcaga aaaatcacta agatgtatat cgataatttt ttcaaaaaaa acatcaattt
     6121 gctcttgtgc tctttgagcc aaagtcgcaa cttttccaac ttctctatta gatattactt
     6181 taataaggtt tgggtctata tattgaagag tgtcttcttc aatagaaata tttaaataat
     6241 cagttgattt agcatcatga ttgaatttgg tacatactag tgtcactcta tatcatcctt
     6301 ctgtaagttt tttgtttact tccaatatag aatgatgtat tattgtaatt gtcgatttac
     6361 aataagttgt tattctaggt ggaagtttat tgcttttgat gtatagatag acttaacctt
     6421 ttcattatag catactatag aagaaaatgc aagcaaaagc atataaacaa caaatgttgt
     6481 ttatatgctt ttgctttttt gttaattgga gaggacaagg tgaatattaa cagtcaaaat
     6541 ccagttatgt cggttaatga atcgatgaaa attttcaaag ctcaagtaat gcttagaaat
     6601 ttaaagctat taagcgagtg gattgttaaa ggggactgaa gttaaagaca ctttttaaga
     6661 agatttttca attaaagggt atattaaaac tttaatttag tactttatat ttttcttgac
     6721 tctttaataa tcctatatac agtagaacga ctcactcctg tttgttcagc aatttcttca
     6781 ccagacagct tttgttcatc atataagaat ttgatctctc ttttcttatg atctggtagt
     6841 gaaggtcttc caccttttct ccctcttgct cttgcagctt ccaaaccttt tttagttcgt
     6901 tcacttaata aattagcttc aagttcagcg aatgcactca tcatggtaaa gaacatcttg
     6961 cccatcgcat ctttcgttga cacattcata tctataatat gtaaatcaat gctattttca
     7021 tccaaccatt gagatagctc aattaattgt tttgttgtac gtccaagacg atctaattta
     7081 tagacaacta gaatatctcc ttcacgtaaa taatctaaac acttatcaag ttccgttctt
     7141 ttagttttac gaccactcgc tttctcgcta aagatacgtt cacaaccata ttcctcaagt
     7201 gtatcaatct gtccatcaag actttgatct tgtgttgaca cacgtgcata accaatttta
     7261 gccatttgaa caccaccttt tttaatcgtt ttatgggtac ttactaatga aatatagttc
     7321 tcgatgtaaa taaacacgtc tgtcagcatc taaaaactca tgattttcga tatttatgat
     7381 acattgatta taacacaagt ttatagactt aaatatccaa gaaaaacctc ttaatattaa
     7441 gttaagaggt tttgtgtcaa aaacgaacgt aatttagaaa taatatttat tggtatttca
     7501 agaatatata tataattatt tactaagtcc atttaaaaaa atttgagaga acttgttcat
     7561 aagtttaatt ctttcattaa tattttgttc atgtgtaaag gtaacaattc cattaacagc
     7621 gtttgctgct attttactaa cagcattaac atcattaata caccattcgc catttaaatt
     7681 accctcctta aaaattacat gataagcatc tatatatttg ttttctaatt tattcatttt
     7741 ttcattaatg ctattagttt tataatattc agtgtaaaat tcaattattg cgttttgaag
     7801 tggataatag tattcggttg ttaaagatag ttcattatat aaataaaatt tttctctatt
     7861 agttttacat ttgatttgtt cctttttcca ctgttcttgc catttagatt cttctatatt
     7921 taaaatttct aaaaatagat tttcttttgt tttaaagtga taataaagat tccctttact
     7981 actttctgat aatttaacaa tttctccagt agtagtggca ttatatccat tttttataaa
     8041 taattccttt gcgacaccta gtattttatc tttcaagttc atcacgcctt taatgagtat
     8101 taagtactat caatgatagc acaaaaaatt caaagaataa ttgtataaca caatacagcc
     8161 atttaaattt cgcaagattt tttgttgtaa tatgtaaaaa aaatagatta taatccttat
     8221 agaccgatcg cacggtctat aaggattgga gggaacttaa atgatttcat tttttacaaa
     8281 aactactgat atgatgacat caaaaaaaag atggactgca ctagtagtat tagctgttag
     8341 tttgtttgtt gttacaatgg atatgacaat attaattatg gctttaccgg aattagtaag
     8401 agagttagag ccttctggta cccaacagtt atggatagtt gatatatact ctcttgtttt
     8461 agctggcttt ataattccat tgagtgcctt tgctgataaa tggggaagaa aaaaagcatt
     8521 attaactgga tttgctttat ttggcctcgt ttcattagct atatttttcg cagaaagtgc
     8581 agagttcgta atagctattc gatttttact tggtattgca ggtgctttaa taatgccaac
     8641 tactctttca atgataagag taatttttga aaaccctaaa gaaagggcca ctgcattagc
     8701 tgtatggtca atcgcttcat cgataggtgc tgtttttgga ccaattatcg gaggagcttt
     8761 acttgagcaa ttttcatggc actcggcatt tttaattaat gtaccgtttg cgataatagc
     8821 agttgtagca ggtttatttt tattaccaga gtctaagtta tcaaaagaaa agtctcactc
     8881 gtgggatatt ccttctacaa ttttatcaat tgcaggcatg attggactgg tatggagtat
     8941 caaagaattt tcaaaagaag gactagcaga tattattcca tgggttgtaa tagtattagc
     9001 aattaccatg atagtgatat ttgttaaacg taatttatca agttctgatc caatgttaga
     9061 cgtaagactt tttaaaaaga gatcattttc agctggtaca attgctgcat ttatgacaat
     9121 ttttgcaatg gcatctgttt tgttattagc ttcacaatgg ttacaggttg tggaagaact
     9181 ttctcctttt aaagctggct tatacctatt acctatggca ataggagata tggtgtttgc
     9241 accaattgca cccggattag cggcgcgatt tggaccgaaa atagtgttac cttccggaat
     9301 tggaattgca gccattggca tgtttattat gtatttcttt ggtcatccat tatcatattc
     9361 tacaatggct ttagcattaa ttttagttgg agctggtatg gcttcactag cagttgcatc
     9421 tgctctaata atgttagaaa cacctacatc aaaagcaggt aatgcagctg ctgttgaaga
     9481 gtctatgtat gaccttggaa atgtttttgg tgtagcagta cttggtagcc tatcttctat
     9541 gctttatcgt gtatttttag atatttcatc tttttcatca aaaggtatag ttggagattt
     9601 agctcatgta gctgaagaat ctgtagtggg cgctgtcgaa gtagctaaag ctacggggat
     9661 aaaacagctt gcaaacgagg ctgtaacatc atttaatgat gcttttgtag caactgcttt
     9721 agtaggtggg attatcatga ttatcatttc aatagttgtc tatttgttaa ttcccaaatc
     9781 acttgatata actaaacaaa aatagtacta aacatatatt tttagatatt gtagattagt
     9841 ttatttttaa aattagtaat agttaaaaag tagaaccatt aattttaaat ggttctactt
     9901 ttttattatt aagtaaaatt ataaaagaca atttcttaaa ttaataatat gatataccta
     9961 tatctaataa tttattagta ttaataaacc ttgaaaggat gattaactga tgaatagagc
    10021 agatattttt aaaatatttg atgtagaact taatataact atatttttag gagcactagt
    10081 agcaactttc atctatttta taagttacat gaacaatgta aatgaattaa ttacattatg
    10141 cttaacatta gcttttcctg catatttatt aaaaattagc agatcgttcg aggttatttt
    10201 attagaggaa aaaataaaaa atagtaaaat caaaatctgt gacaaagata gaattgaacg
    10261 agagatactg gtgaaaaaga acctaataga tgaactattg tttcctaata gagttattaa
    10321 aaaatataca ctgacaattt taatttacag tataggtatt attattcttt tactactttg
    10381 taatatgtta agttatggaa ttggtgaaaa tgtcaatttt atctggagat tactagcttg
    10441 ttatgtaacg agtgtcacag tgattatagt tgccttgatt ataatgataa tgatatttga
    10501 tcttataatt ggattactat taaattttca aatattttta aaaaataaaa agtatgatga
    10561 ttttttataa ttattcgatt tatataatga aaaattaaat gattaaacat agaaacctca
    10621 aaacaaacca tatttaatag agtttatggc gccttataca aataaaggct atgctgttaa
    10681 agaattatgt agctatcttc agattaatat ggatgatgtt tatactattg gcgatagaaa
    10741 attgatttac ctatgtttaa taccctagat aattcttctg ataatttagt ttttgttaga
    10801 gtttaacata actgatgatt atcagaagtt tgattcacat taaaaaggca caacaagttc
    10861 atgaacttgt gtgccttttt aattgcaatt agttaccaaa taaacgactc caaaatctct
    10921 ttttaggtgg ttctacctcc ctattatcac tggaatcgtt cttcgttttt tcaacaaact
    10981 tatcctcttt tgttaattca tctgtactgt catgcttatt ctcttcagtt atatttttat
    11041 gatttacttc ggttggttga cattcttttt gttgttggtt gatattttga gattcttcag
    11101 taaaagttgc ttcttgtgga ttaatattct gtctattatt catcgttgta tcaaaagaat
    11161 atttaagctg tctttcttct tctaattggc tttctagttt ttgaattttt ttattgtttt
    11221 ctaaagataa tatttgttga ttctccaaca gtttatttaa tgtattaaca ttgctatttt
    11281 gttgatctaa ttgtttagtt agattctcaa tatatttttc atcattttta gctctagttt
    11341 caaagatttc tatttgcttt tctaattcgt ttacttttgt ttttaatgtc tcagaactat
    11401 ttgaattatt atgtatatac ttttcaactt ttgcagtatt ttcattaaat aattcatttt
    11461 ttactgattc tttattatga gttgttttct ctttcttagt gattttttct ataattttta
    11521 aataatcatt atctttaata taattaattc cattttcttt ttcaaaagat atatttaatc
    11581 ttttagcatt attaacaata gtttgtttag ttacacctaa ttcatcagca atcattttaa
    11641 tagttttcat aacatccccc cttaggtacc taatttagta cctaaatttt accacctaac
    11701 aaaatttatt tcatcattta gtactaaatt tactatctat taactaccta aatgcctaat
    11761 tatctatcct taaaatattt tcgccactta attaggtgct aaaatagcga attatacgtt
    11821 tggtagtttt aggtgtactt ttaattacat ttaaaactct ttatatacgc cattaaaagt
    11881 gttaatatta ctttataaat attaaaagag tcgatgctat tggcgtagca tcgactctcg
    11941 gtaataaaac gattcgcact cgtttgttta tatatttttt tgatacttgt attatatata
    12001 tctaatcatc taagtgcaag cacaaaacat ataacttacg taaaaattgt tttattacct
    12061 caatcccaaa atggaaatga ggtttttatt atgcccaatt ttgaaaaata taatttatca
    12121 caagtaaaaa ctgaaagatt ttatcaactg cctaaatatt tatttgaaga tgcatatttt
    12181 aagaaaatgt ctgcagaagc caaaattatg tatgcgttat taaaagatcg ttttgaatta
    12241 tccctccaaa atgaatgggt agataaaaat aataatattt actttatttt cagtaataaa
    12301 catttgtgtg aatacttagg ttatgcagaa caaaaaatta taaaattaaa aaaagagtta
    12361 ataaaattta atttactaac tcaagaacgt gttggcctta ataaaccaaa tagattatac
    12421 ctattaaaac ctaattatga cattgaagcc agtcatatca aggaacttcc aaattcacag
    12481 ttccagaaca atgaatttgg aagttctaga actgtgaatt taagtggtca agaacttcca
    12541 aattcacagt ctaatgatac tgattataat gacactgatt atattaagac taattataat
    12601 gatatgtatg atttgaatga taggaaatta acatattcta gtaatcatac aaatcattca
    12661 aatcactact attcaaaatt caatgatgaa gctttaaaat ttcaattact tgaagaacta
    12721 ccacaaagta ttcaaagtta tttaagtaac ttttctgtat ctgaaattaa acttattaaa
    12781 tctgtgttat taaaagccaa aacatccttc aacaattcta ttgatgcata ttatttatta
    12841 gaagatatgg aatttgaaat tgttaatgtt cttaagcgtt ttaaagctac attaattcaa
    12901 aaaaatgaaa ccgttgaagc aatgcaaggg tacttaatga aatctcttaa atctgaattt
    12961 gcagaagtac atacgcttaa taaacgacgt gatcatttac caatcacttc tttatttaat
    13021 caataataca acttaataag ccaacaatga tccaattaga gaatatcttt aattagatgt
    13081 tctcttaact gtaagcgaac tttattttca atgaaatgta attaattaag taagtgggac
    13141 taaaactttt cttttttagt cccattttta taaacaatta ttattcattg ttactactat
    13201 cagcaatttt ttctttttag tgaaacccaa gtatttgtaa gcatgataaa ttacttcttt
    13261 ttatatttat tgggtttatt tgtattaatg tagttggaat acatctttaa caattcttct
    13321 gtttcatcag gacttaaatg ctcacattca aaatactttt caagcattgc accttttcga
    13381 atcagtctat gtgctctttc ttttctatct aaatttttta atctatgctt tcttaattct
    13441 gctttttcaa tttcagcatt tatttttttg agatgtttaa aatcattcat cgtatcactt
    13501 actattctag ttaattttgt cttctgttaa attttccttg ttctcgttgc tgctattttg
    13561 ttcgatatct
//
