LOCUS       NZ_CP073848            13570 bp    DNA     circular CON 16-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1266875 plasmid p1B1266875,
            complete sequence.
ACCESSION   NZ_CP073848
VERSION     NZ_CP073848.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699880
            Assembly: GCF_019329585.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 391x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019329585.1-RS_2024_04_16
            Annotation Date                   :: 04/16/2024 07:55:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,679
            CDSs (total)                      :: 2,598
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 42 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 27 of 78
            Pseudo Genes (multiple problems)  :: 24 of 78
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073848.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1266875"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p1B1266875"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(13517..13570,1..216))
                     /locus_tag="KFV50_RS13225"
                     /old_locus_tag="KFV50_13235"
     CDS             complement(join(13517..13570,1..216))
                     /locus_tag="KFV50_RS13225"
                     /old_locus_tag="KFV50_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            425..2347
                     /locus_tag="KFV50_RS13230"
                     /old_locus_tag="KFV50_13240"
     CDS             425..2347
                     /locus_tag="KFV50_RS13230"
                     /old_locus_tag="KFV50_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            2621..3034
                     /locus_tag="KFV50_RS13525"
                     /old_locus_tag="KFV50_13245"
     CDS             2621..3034
                     /locus_tag="KFV50_RS13525"
                     /old_locus_tag="KFV50_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            3111..3344
                     /locus_tag="KFV50_RS13240"
                     /old_locus_tag="KFV50_13250"
     CDS             3111..3344
                     /locus_tag="KFV50_RS13240"
                     /old_locus_tag="KFV50_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(3393..4097)
                     /locus_tag="KFV50_RS13245"
                     /old_locus_tag="KFV50_13255"
     CDS             complement(3393..4097)
                     /locus_tag="KFV50_RS13245"
                     /old_locus_tag="KFV50_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(4248..4946)
                     /locus_tag="KFV50_RS13250"
                     /old_locus_tag="KFV50_13260"
     CDS             complement(4248..4946)
                     /locus_tag="KFV50_RS13250"
                     /old_locus_tag="KFV50_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            5110..5208
                     /locus_tag="KFV50_RS13255"
                     /old_locus_tag="KFV50_13265"
     CDS             5110..5208
                     /locus_tag="KFV50_RS13255"
                     /old_locus_tag="KFV50_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(5385..6296)
                     /locus_tag="KFV50_RS13260"
                     /old_locus_tag="KFV50_13270"
     CDS             complement(5385..6296)
                     /locus_tag="KFV50_RS13260"
                     /old_locus_tag="KFV50_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(6715..7275)
                     /locus_tag="KFV50_RS13265"
                     /old_locus_tag="KFV50_13275"
     CDS             complement(6715..7275)
                     /locus_tag="KFV50_RS13265"
                     /old_locus_tag="KFV50_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            complement(7526..8092)
                     /gene="qacR"
                     /locus_tag="KFV50_RS13270"
                     /old_locus_tag="KFV50_13280"
     ARG             complement(7530..8092)
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            8271..9815
                     /gene="qac"
                     /locus_tag="KFV50_RS13275"
                     /old_locus_tag="KFV50_13285"
     CDS             8271..9815
                     /gene="qac"
                     /locus_tag="KFV50_RS13275"
                     /old_locus_tag="KFV50_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            10020..10580
                     /locus_tag="KFV50_RS13280"
                     /old_locus_tag="KFV50_13290"
     CDS             10020..10580
                     /locus_tag="KFV50_RS13280"
                     /old_locus_tag="KFV50_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(10899..11660)
                     /locus_tag="KFV50_RS13290"
                     /old_locus_tag="KFV50_13300"
     CDS             complement(10899..11660)
                     /locus_tag="KFV50_RS13290"
                     /old_locus_tag="KFV50_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            12101..13036
                     /locus_tag="KFV50_RS13295"
                     /old_locus_tag="KFV50_13305"
     CDS             12101..13036
                     /locus_tag="KFV50_RS13295"
                     /old_locus_tag="KFV50_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            complement(13261..13500)
                     /locus_tag="KFV50_RS13300"
                     /old_locus_tag="KFV50_13310"
     CDS             complement(13261..13500)
                     /locus_tag="KFV50_RS13300"
                     /old_locus_tag="KFV50_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     ARG             8272..9812
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          12102..13036
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          426..2278
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 ttcgatatct ttattaaatt ctccaatgac tttatccata attttataca attcatcact
       61 agagttgatc atatttaaat caagtttatt taaaaaataa ccaccaattt tattagatag
      121 ttgttcttct tgttttttct tttgctcttt cagttgtgca atttgttttt ctaattgact
      181 taattttgta atatctgttt gtttaatttt agacattaaa aaactccctt agtttatcta
      241 tataaagcat accataattc attgggtaag tcgcacatcc atggtataat ataaccagtt
      301 cttaagacac tgcactgccc atttacatgc aaattgaaaa ttggcataaa aatgggcaac
      361 catactggtt gatcattata gttcccacag gggtaaaaaa acagaaaaga gaggaggtaa
      421 aatcatgaat aaaggttact ttatgttgag agtgactaac attaatcgag caacaaaaaa
      481 tatcgttgcg tcagcgtctt atcgttctga tgaagcatta tatagcgaac gtacagatga
      541 aaaaattaag tttagaaatc atactattaa gcctgaaagc atgattttaa ctcctcaaca
      601 tgcacccgaa tggactaaag atagacagcg tttatggaat gaagttgata aagttgaaaa
      661 gaataatgcg aaaacaaaaa atcctagatt agcaaaagaa gtcttattaa gtttgcctaa
      721 tgattttgat agagaggtac aaactgaatt aactaaagat tttataaaaa gtgagtttgt
      781 agacaaagga atggttgctg atatttcaat tcatagagat gatataaaca acccacacgc
      841 ccatgttcta cttactcaaa gaccgtttaa tgcagacggt acttggggga aaaagacaaa
      901 aacgagaacg caatatgatg aaaatggcaa tgcgatttta aataaaaatg gtaataaagt
      961 tagaaaacaa gaacgatttg ccgactttga tttcaaagaa gttcgtaagc attgggaatt
     1021 aaaattaaat caatactcag aacgtgaaca aagtttaaga cgatatgata gtcgttcttt
     1081 tgaagcgcaa ggtttagaaa aaattgccga aattccactt acaagagaag aatatagatt
     1141 agaacaaaat gaaagaaaac gctgtgaaaa agagggtatc gagtaccaac ccgttactta
     1201 ttacggacag aaaaatgaac aaattagaag atataatcgt ggcgaagtat cacaattatt
     1261 tagtgataaa gagaaagaac aagcacaaaa tgaaattaga ttatttattc aaaatgctaa
     1321 cgaacaagta agtaaaaatg aacaaagtta ttctatttta tttgatagat atcaacgtga
     1381 tgttggctat tcagaggcga aagaaacaat taaaaatacg tttgacagtg cgtctacctt
     1441 tggtcgtaaa attcaaaatg atttacttaa aaatgaaatt aagagacgtc aattaaaatt
     1501 ctatgctaaa aattatgatg aaaattatca gtttagaaaa gaaattgaaa gtaaaggata
     1561 tagtcaacaa gagtttgtta atactgttga aactaactat aaagcgttac tcaatgagaa
     1621 gaataaatta gaaagtagag aggaaaaacg taaagagatt tatcaatcag ctaaagatgt
     1681 ttatctaact gaaagtgaaa aaaatcatct cattgttcag caaatgtacc ctaaatctca
     1741 tcatcatttt agccatgatg aaaaagcatt tattgttgat gaggcacaaa aaggtaatat
     1801 tataaaagca actcatgttc aatacctgtt caaacaaaat aacaatattc ctaatgatgt
     1861 attgctagaa gatacttata aaaaagcgag taaagatatt ttctttagtc aaaaacatct
     1921 taaaaattta gaaaaagata gctttgaata taacgttgag ttgcaattta ttaaggaaaa
     1981 agaaaaagaa attgatagtt ataaagaaca tattgatgat gatttaagaa tagatatagg
     2041 cgataaaaac tttgaaaaag tcaaagacca aacacctaat gaaaaagttc aattactcat
     2101 taaacttgag aaatataatc atgtcgatca agaagaagtt atcgaaaaac atattaatca
     2161 agtagataga aatgatcatg acaatcgtat tgaacatgaa aatgaacaaa ctaatcacat
     2221 taatttacca acgataaatg ctagtgactt tcttatgtac tatgcgaacg aaactgaaaa
     2281 gcaaaacaat attaatcatc agaaaaagaa gaagaaacaa gagagaaata accaaatgag
     2341 aagatagtaa gcagttgaaa tcatacaaag ttcattcttt aataataaga aacttaaccc
     2401 aatgtggtaa attagctttg gtttaaagaa aacatttata tacttagata aggtacattc
     2461 tatgtattat cacaatgtat tttaaaacgt gtataattat agaaaaacct tgatattatg
     2521 cgattgttca catatttcag cataaaaaat actaatgttc ataaatatcg atagaaaaaa
     2581 ttgagtttaa atatatctta aagttaagga gaaaaataat ttggaaacaa aaattggaca
     2641 tcaaatacaa aagttaagaa aaagtaaaaa catgtctcaa gaaaaattag ctgaaaagtt
     2701 aggggtatcg agacattcta tttctaattg ggaaagagaa gtaagtaatc ccgacttaaa
     2761 aacaatttta gaaatcacta aattatttaa tgtttctcta aatcaattaa ttaaaggagt
     2821 ggaaatcatg caagttaata aatatgttta ctctgcatta gcattttttc taggaggttt
     2881 cggggctcat aagttttatg ttaagaaaaa taaaaatgct cttctttatt tactattttg
     2941 ttggacggga ataccgggtg tgataggtat ggtagaaggt gttttaacac ttatgcgtcc
     3001 gagcgatagt gaaaacaata tagaaataaa ttaaaccaaa aataaaaagc cgatgtatcg
     3061 gatttttttt agtgtacatg tgaatattaa aaatgatgga gtgatttcac atgaacacat
     3121 tcgttttcgg cgtgattaca aagcgattag aagaaatagg ttttaatgta cacaaggtgt
     3181 ttaaaatgcc cgaaaaacag gcactatttg aagatttgta ccattatgtg agatacgtta
     3241 aaccatgcaa ggctgaacta acaatggtgg cattagatag tgatgaattg attcattact
     3301 ttatatgctg ttacatggaa cgttatgctt atcttttgaa ctaatgataa aaaagacgcc
     3361 taattttagg cgccttttta ataatctact aactacttat tatatctaat tttacttgct
     3421 aacttagcta ctgttttatt gtcgtagggt ttaatataga ctgtttcaat attctcctct
     3481 attgcttttt ttgttatctt attcatttga ctataatcac tattttgtga attttcccat
     3541 ttataaggga tattattata tacattaatt gttccatcac cattactgct atatgttaca
     3601 gacccaccag ctctcatagg agcttctaat ttcacaactt tttctttata aattgcacta
     3661 tttttggctt gtggatttac ttttgagcct tttggtattt ctgtaacata aacattttta
     3721 atacctttta aatcatttct tgtagatatt aactgatacc aaactcgtgc atattcaatt
     3781 tctttcgagt taaaattatt taagtaactt ttatttttat cagtagattg ttgcttctta
     3841 ttactatcat tatttttttc ttgtttttta ttatcatttt tttctttatt ttgtttatta
     3901 ctactttctg aattatccgc actatcagat gtgttagttt tttcgtcaat ttcatttgat
     3961 gcttcattat ctttgttttc tgaggtgtta gtatcactag aattattatc attgtgatgt
     4021 tctgaatcgt tattattaca actcactaaa attaccgata aactaacaat taatataggt
     4081 aaaatgattt ttttcatatt acaactcctt ttcagtattt caaattagat tatgttaata
     4141 ttttttcagc aagaaaacgt aaattacatg agttgaaaaa tatgtattaa atatataata
     4201 aaataaccac atgttttaca taaacacggt ggttatttta tcaattttta tattgccttt
     4261 tttctgaata ttaaaagact tatacctaat attagtagtc cagaagcgcc aattattgac
     4321 aatatatcac tttggttgct ttctcctgtt tctggtaata cttgagagtt tgaatcttga
     4381 tttttcattc cttcagaact cttgttttta ttttcagtta caggtgtcaa cacaccttga
     4441 ggtgtattaa tagttttatt ttcttcatta gctatctttt gactttcatt ttttacaatt
     4501 tgattatatt tttctgtgga tacactatta gggttaggaa catgactttc taattcttgg
     4561 tattcttctt gagtcataca attaggtatc acccctccga aaggtctttt accttgttga
     4621 gttctctcat tattttgtat atcagtactt tgtgattgat attcattaaa tacacttgaa
     4681 actttctcat tatactcgat ttgagttaat tcccctgaat ctttctgttt agaaagttca
     4741 cttatttgat tttgtgcaga ttgtgggagg gatgaagtat tgaagtctac attagcccaa
     4801 ccatctccat ttgtatctgc gctatttggt aagttctctc cgtatacggt tttggcacca
     4861 tcctcagcat tggctgtcgc tgaaaaacct gtgaatgtta atgagctagc caataataaa
     4921 gttggaagta atgatcgttt tttcataaaa ttatctcctt taatagaatt tgtattttca
     4981 ttatcacata gatacattaa acataaaatg acttatcgga agtgcatatg cttaacaatt
     5041 cacaaatgat ataatataaa agtcgtcgtt ctacttctgt gctggtttag catggaagga
     5101 ggtgacacca tgttcagcat catatttgta tctatagtag ctcctattat agtaggggtt
     5161 ataatcacgt tgttttcaca ttggttgaat aatcgtgata aacaataaag cgacgacaca
     5221 gtaataacac caaaaagagc ccgataacta ctggaatagt tatcgggctc ggtgtttacc
     5281 atgttcaaca catatttgtt atctaaatta taacatcata gtgtggagga atgcaaaatt
     5341 gaataattag tatgttaagt ctatactata ctgattagta agtactaatt atcatctttg
     5401 ttattgttac ccctttcatt ggcttttgaa tctaattctt tttgaaattt tttgtgtgat
     5461 tttgaagaag tgtttaaaaa gaaataggtc atattatcca tacgatgtcc ttttataaat
     5521 tcattgataa attgtttttt tgtcattttt ttggttgtat tacgttgttt ttgcaatcta
     5581 atgatttctt cttcaatgta tactcttatc atcaaatcta aactagatgt ctgattataa
     5641 gtagtgaata attttggaac taataaagtt ttattaccat caaaaagaaa tggagtgaaa
     5701 tcataagttt cccatatacc gttagtagag aaataataat atgtcacagt ttctttagaa
     5761 agaaacgatt tcatttctgg aaatgactga atcatggatt tattaaaatc aattataaca
     5821 ttcatgatta tattacttgt taaatctgat aatttatcat caccaaaatt tctgattaat
     5881 ggtgcttttt gaggtattgt taatccgtca tcatttaaga taatggaaag aatatcttca
     5941 ttcataaata gattaatcaa ttcagatgct gtagtacctt ttcctgtatt acctttctga
     6001 gaaaaaccta agcgtaaatg gtgtggttct gaaaaatgag agaataattt tctaaattca
     6061 ttacgctttt gggtatcaga aaaatcacta agatgtatat cgataatttt ttcaaaaaaa
     6121 acatcaattt gctcttgtgc tctttgagcc aaagtcgcaa cttttccaac ttctctatta
     6181 gatattactt taataaggtt tgggtctata tattgaagag tgtcttcttc aatagaaata
     6241 tttaaataat cagttgattt agcatcatga ttgaatttgg tacatactag tgtcactcta
     6301 tatcatcctt ctgtaagttt tttgtttact tccaatatag aatgatgtat tattgtaatt
     6361 gtcgatttac aataagttgt tattctaggt ggaagtttat tgcttttgat gtatagatag
     6421 acttaacctt ttcattatag catactatag aagaaaatgc aagcaaaagc atataaacaa
     6481 caaatgttgt ttatatgctt ttgctttttt gttaattgga gaggacaagg tgaatattaa
     6541 cagtcaaaat ccagttatgt cggttaatga atcgatgaaa attttcaaag ctcaagtaat
     6601 gcttagaaat ttaaagctat taagcgagtg gattgttaaa ggggactgaa gttaaagaca
     6661 ctttttaaga agatttttca attaaagggt atattaaaac tttaatttag tactttatat
     6721 ttttcttgac tctttaataa tcctatatac agtagaacga ctcactcctg tttgttcagc
     6781 aatttcttca ccagacagct tttgttcatc atataagaat ttgatctctc ttttcttatg
     6841 atctggtagt gaaggtcttc caccttttct ccctcttgct cttgcagctt ccaaaccttt
     6901 tttagttcgt tcacttaata aattagcttc aagttcagcg aatgcactca tcatggtaaa
     6961 gaacatcttg cccatcgcat ctttcgttga cacattcata tctataatat gtaaatcaat
     7021 gctattttca tccaaccatt gagatagctc aattaattgt tttgttgtac gtccaagacg
     7081 atctaattta tagacaacta gaatatctcc ttcacgtaaa taatctaaac acttatcaag
     7141 ttccgttctt ttagttttac gaccactcgc tttctcgcta aagatacgtt cacaaccata
     7201 ttcctcaagt gtatcaatct gtccatcaag actttgatct tgtgttgaca cacgtgcata
     7261 accaatttta gccatttgaa caccaccttt tttaatcgtt ttatgggtac ttactaatga
     7321 aatatagttc tcgatgtaaa taaacacgtc tgtcagcatc taaaaactca tgattttcga
     7381 tatttatgat acattgatta taacacaagt ttatagactt aaatatccaa gaaaaacctc
     7441 ttaatattaa gttaagaggt tttgtgtcaa aaacgaacgt aatttagaaa taatatttat
     7501 tggtatttca agaatatata tataattatt tactaagtcc atttaaaaaa atttgagaga
     7561 acttgttcat aagtttaatt ctttcattaa tattttgttc atgtgtaaag gtaacaattc
     7621 cattaacagc gtttgctgct attttactaa cagcattaac atcattaata caccattcgc
     7681 catttaaatt accctcctta aaaattacat gataagcatc tatatatttg ttttctaatt
     7741 tattcatttt ttcattaatg ctattagttt tataatattc agtgtaaaat tcaattattg
     7801 cgttttgaag tggataatag tattcggttg ttaaagatag ttcattatat aaataaaatt
     7861 tttctctatt agttttacat ttgatttgtt cctttttcca ctgttcttgc catttagatt
     7921 cttctatatt taaaatttct aaaaatagat tttcttttgt tttaaagtga taataaagat
     7981 tccctttact actttctgat aatttaacaa tttctccagt agtagtggca ttatatccat
     8041 tttttataaa taattccttt gcgacaccta gtattttatc tttcaagttc atcacgcctt
     8101 taatgagtat taagtactat caatgatagc acaaaaaatt caaagaataa ttgtataaca
     8161 caatacagcc atttaaattt cgcaagattt tttgttgtaa tatgtaaaaa aaatagatta
     8221 taatccttat agaccgatcg cacggtctat aaggattgga gggaacttaa atgatttcat
     8281 tttttacaaa aactactgat atgatgacat caaaaaaaag atggactgca ctagtagtat
     8341 tagctgttag tttgtttgtt gttacaatgg atatgacaat attaattatg gctttaccgg
     8401 aattagtaag agagttagag ccttctggta cccaacagtt atggatagtt gatatatact
     8461 ctcttgtttt agctggcttt ataattccat tgagtgcctt tgctgataaa tggggaagaa
     8521 aaaaagcatt attaactgga tttgctttat ttggcctcgt ttcattagct atatttttcg
     8581 cagaaagtgc agagttcgta atagctattc gatttttact tggtattgca ggtgctttaa
     8641 taatgccaac tactctttca atgataagag taatttttga aaaccctaaa gaaagggcca
     8701 ctgcattagc tgtatggtca atcgcttcat cgataggtgc tgtttttgga ccaattatcg
     8761 gaggagcttt acttgagcaa ttttcatggc actcggcatt tttaattaat gtaccgtttg
     8821 cgataatagc agttgtagca ggtttatttt tattaccaga gtctaagtta tcaaaagaaa
     8881 agtctcactc gtgggatatt ccttctacaa ttttatcaat tgcaggcatg attggactgg
     8941 tatggagtat caaagaattt tcaaaagaag gactagcaga tattattcca tgggttgtaa
     9001 tagtattagc aattaccatg atagtgatat ttgttaaacg taatttatca agttctgatc
     9061 caatgttaga cgtaagactt tttaaaaaga gatcattttc agctggtaca attgctgcat
     9121 ttatgacaat ttttgcaatg gcatctgttt tgttattagc ttcacaatgg ttacaggttg
     9181 tggaagaact ttctcctttt aaagctggct tatacctatt acctatggca ataggagata
     9241 tggtgtttgc accaattgca cccggattag cggcgcgatt tggaccgaaa atagtgttac
     9301 cttccggaat tggaattgca gccattggca tgtttattat gtatttcttt ggtcatccat
     9361 tatcatattc tacaatggct ttagcattaa ttttagttgg agctggtatg gcttcactag
     9421 cagttgcatc tgctctaata atgttagaaa cacctacatc aaaagcaggt aatgcagctg
     9481 ctgttgaaga gtctatgtat gaccttggaa atgtttttgg tgtagcagta cttggtagcc
     9541 tatcttctat gctttatcgt gtatttttag atatttcatc tttttcatca aaaggtatag
     9601 ttggagattt agctcatgta gctgaagaat ctgtagtggg cgctgtcgaa gtagctaaag
     9661 ctacggggat aaaacagctt gcaaacgagg ctgtaacatc atttaatgat gcttttgtag
     9721 caactgcttt agtaggtggg attatcatga ttatcatttc aatagttgtc tatttgttaa
     9781 ttcccaaatc acttgatata actaaacaaa aatagtacta aacatatatt tttagatatt
     9841 gtagattagt ttatttttaa aattagtaat agttaaaaag tagaaccatt aattttaaat
     9901 ggttctactt ttttattatt aagtaaaatt ataaaagaca atttcttaaa ttaataatat
     9961 gatataccta tatctaataa tttattagta ttaataaacc ttgaaaggat gattaactga
    10021 tgaatagagc agatattttt aaaatatttg atgtagaact taatataact atatttttag
    10081 gagcactagt agcaactttc atctatttta taagttacat gaacaatgta aatgaattaa
    10141 ttacattatg cttaacatta gcttttcctg catatttatt aaaaattagc agatcgttcg
    10201 aggttatttt attagaggaa aaaataaaaa atagtaaaat caaaatctgt gacaaagata
    10261 gaattgaacg agagatactg gtgaaaaaga acctaataga tgaactattg tttcctaata
    10321 gagttattaa aaaatataca ctgacaattt taatttacag tataggtatt attattcttt
    10381 tactactttg taatatgtta agttatggaa ttggtgaaaa tgtcaatttt atctggagat
    10441 tactagcttg ttatgtaacg agtgtcacag tgattatagt tgccttgatt ataatgataa
    10501 tgatatttga tcttataatt ggattactat taaattttca aatattttta aaaaataaaa
    10561 agtatgatga ttttttataa ttattcgatt tatataatga aaaattaaat gattaaacat
    10621 agaaacctca aaacaaacca tatttaatag agtttatggc gccttataca aataaaggct
    10681 atgctgttaa agaattatgt agctatcttc agattaatat ggatgatgtt tatactattg
    10741 gcgatagaaa attgatttac ctatgtttaa taccctagat aattcttctg ataatttagt
    10801 ttttgttaga gtttaacata actgatgatt atcagaagtt tgattcacat taaaaaggca
    10861 caacaagttc atgaacttgt gtgccttttt aattgcaatt agttaccaaa taaacgactc
    10921 caaaatctct ttttaggtgg ttctacctcc ctattatcac tggaatcgtt cttcgttttt
    10981 tcaacaaact tatcctcttt tgttaattca tctgtactgt catgcttatt ctcttcagtt
    11041 atatttttat gatttacttc ggttggttga cattcttttt gttgttggtt gatattttga
    11101 gattcttcag taaaagttgc ttcttgtgga ttaatattct gtctattatt catcgttgta
    11161 tcaaaagaat atttaagctg tctttcttct tctaattggc tttctagttt ttgaattttt
    11221 ttattgtttt ctaaagataa tatttgttga ttctccaaca gtttatttaa tgtattaaca
    11281 ttgctatttt gttgatctaa ttgtttagtt agattctcaa tatatttttc atcattttta
    11341 gctctagttt caaagatttc tatttgcttt tctaattcgt ttacttttgt ttttaatgtc
    11401 tcagaactat ttgaattatt atgtatatac ttttcaactt ttgcagtatt ttcattaaat
    11461 aattcatttt ttactgattc tttattatga gttgttttct ctttcttagt gattttttct
    11521 ataattttta aataatcatt atctttaata taattaattc cattttcttt ttcaaaagat
    11581 atatttaatc ttttagcatt attaacaata gtttgtttag ttacacctaa ttcatcagca
    11641 atcattttaa tagttttcat aacatccccc cttaggtacc taatttagta cctaaatttt
    11701 accacctaac aaaatttatt tcatcattta gtactaaatt tactatctat taactaccta
    11761 aatgcctaat tatctatcct taaaatattt tcgccactta attaggtgct aaaatagcga
    11821 attatacgtt tggtagtttt aggtgtactt ttaattacat ttaaaactct ttatatacgc
    11881 cattaaaagt gttaatatta ctttataaat attaaaagag tcgatgctat tggcgtagca
    11941 tcgactctcg gtaataaaac gattcgcact cgtttgttta tatatttttt tgatacttgt
    12001 attatatata tctaatcatc taagtgcaag cacaaaacat ataacttacg taaaaattgt
    12061 tttattacct caatcccaaa atggaaatga ggtttttatt atgcccaatt ttgaaaaata
    12121 taatttatca caagtaaaaa ctgaaagatt ttatcaactg cctaaatatt tatttgaaga
    12181 tgcatatttt aagaaaatgt ctgcagaagc caaaattatg tatgcgttat taaaagatcg
    12241 ttttgaatta tccctccaaa atgaatgggt agataaaaat aataatattt actttatttt
    12301 cagtaataaa catttgtgtg aatacttagg ttatgcagaa caaaaaatta taaaattaaa
    12361 aaaagagtta ataaaattta atttactaac tcaagaacgt gttggcctta ataaaccaaa
    12421 tagattatac ctattaaaac ctaattatga cattgaagcc agtcatatca aggaacttcc
    12481 aaattcacag ttccagaaca atgaatttgg aagttctaga actgtgaatt taagtggtca
    12541 agaacttcca aattcacagt ctaatgatac tgattataat gacactgatt atattaagac
    12601 taattataat gatatgtatg atttgaatga taggaaatta acatattcta gtaatcatac
    12661 aaatcattca aatcactact attcaaaatt caatgatgaa gctttaaaat ttcaattact
    12721 tgaagaacta ccacaaagta ttcaaagtta tttaagtaac ttttctgtat ctgaaattaa
    12781 acttattaaa tctgtgttat taaaagccaa aacatccttc aacaattcta ttgatgcata
    12841 ttatttatta gaagatatgg aatttgaaat tgttaatgtt cttaagcgtt ttaaagctac
    12901 attaattcaa aaaaatgaaa ccgttgaagc aatgcaaggg tacttaatga aatctcttaa
    12961 atctgaattt gcagaagtac atacgcttaa taaacgacgt gatcatttac caatcacttc
    13021 tttatttaat caataataca acttaataag ccaacaatga tccaattaga gaatatcttt
    13081 aattagatgt tctcttaact gtaagcgaac tttattttca atgaaatgta attaattaag
    13141 taagtgggac taaaactttt cttttttagt cccattttta taaacaatta ttattcattg
    13201 ttactactat cagcaatttt ttctttttag tgaaacccaa gtatttgtaa gcatgataaa
    13261 ttacttcttt ttatatttat tgggtttatt tgtattaatg tagttggaat acatctttaa
    13321 caattcttct gtttcatcag gacttaaatg ctcacattca aaatactttt caagcattgc
    13381 accttttcga atcagtctat gtgctctttc ttttctatct aaatttttta atctatgctt
    13441 tcttaattct gctttttcaa tttcagcatt tatttttttg agatgtttaa aatcattcat
    13501 cgtatcactt actattctag ttaattttgt cttctgttaa attttccttg ttctcgttgc
    13561 tgctattttg
//
