LOCUS       NZ_CP073846            13570 bp    DNA     circular CON 16-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1265603 plasmid p1B1265603,
            complete sequence.
ACCESSION   NZ_CP073846
VERSION     NZ_CP073846.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699879
            Assembly: GCF_019329565.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 260x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019329565.1-RS_2024_04_16
            Annotation Date                   :: 04/16/2024 08:03:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,687
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 42 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 24 of 80
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073846.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1265603"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p1B1265603"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(11896..13570,1..248))
                     /locus_tag="KFV41_RS13245"
                     /old_locus_tag="KFV41_13255"
     CDS             complement(join(11896..13570,1..248))
                     /locus_tag="KFV41_RS13245"
                     /old_locus_tag="KFV41_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            457..726
                     /locus_tag="KFV41_RS13250"
                     /old_locus_tag="KFV41_13260"
     CDS             457..726
                     /locus_tag="KFV41_RS13250"
                     /old_locus_tag="KFV41_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            743..982
                     /locus_tag="KFV41_RS13255"
                     /old_locus_tag="KFV41_13265"
     CDS             743..982
                     /locus_tag="KFV41_RS13255"
                     /old_locus_tag="KFV41_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(1207..2142)
                     /locus_tag="KFV41_RS13260"
                     /old_locus_tag="KFV41_13270"
     CDS             complement(1207..2142)
                     /locus_tag="KFV41_RS13260"
                     /old_locus_tag="KFV41_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            2583..3344
                     /locus_tag="KFV41_RS13265"
                     /old_locus_tag="KFV41_13275"
     CDS             2583..3344
                     /locus_tag="KFV41_RS13265"
                     /old_locus_tag="KFV41_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            complement(3663..4223)
                     /locus_tag="KFV41_RS13275"
                     /old_locus_tag="KFV41_13285"
     CDS             complement(3663..4223)
                     /locus_tag="KFV41_RS13275"
                     /old_locus_tag="KFV41_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(4428..5972)
                     /gene="qac"
                     /locus_tag="KFV41_RS13280"
                     /old_locus_tag="KFV41_13290"
     CDS             complement(4428..5972)
                     /gene="qac"
                     /locus_tag="KFV41_RS13280"
                     /old_locus_tag="KFV41_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            6151..6717
                     /gene="qacR"
                     /locus_tag="KFV41_RS13285"
                     /old_locus_tag="KFV41_13295"
     ARG             6152..6714
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6968..7528
                     /locus_tag="KFV41_RS13290"
                     /old_locus_tag="KFV41_13300"
     CDS             6968..7528
                     /locus_tag="KFV41_RS13290"
                     /old_locus_tag="KFV41_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            7947..8858
                     /locus_tag="KFV41_RS13295"
                     /old_locus_tag="KFV41_13305"
     CDS             7947..8858
                     /locus_tag="KFV41_RS13295"
                     /old_locus_tag="KFV41_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(9035..9133)
                     /locus_tag="KFV41_RS13300"
                     /old_locus_tag="KFV41_13310"
     CDS             complement(9035..9133)
                     /locus_tag="KFV41_RS13300"
                     /old_locus_tag="KFV41_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            9297..9995
                     /locus_tag="KFV41_RS13305"
                     /old_locus_tag="KFV41_13315"
     CDS             9297..9995
                     /locus_tag="KFV41_RS13305"
                     /old_locus_tag="KFV41_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            10146..10850
                     /locus_tag="KFV41_RS13310"
                     /old_locus_tag="KFV41_13320"
     CDS             10146..10850
                     /locus_tag="KFV41_RS13310"
                     /old_locus_tag="KFV41_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(10899..11132)
                     /locus_tag="KFV41_RS13315"
                     /old_locus_tag="KFV41_13325"
     CDS             complement(10899..11132)
                     /locus_tag="KFV41_RS13315"
                     /old_locus_tag="KFV41_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(11209..11622)
                     /locus_tag="KFV41_RS13565"
                     /old_locus_tag="KFV41_13330"
     CDS             complement(11209..11622)
                     /locus_tag="KFV41_RS13565"
                     /old_locus_tag="KFV41_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     ARG             complement(4432..5972)
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          complement(1208..2142)
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11966..13569)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 ttcgcattat tcttttcaac tttatcaact tcattccata aacgctgtct atctttagtc
       61 cattcgggtg catgttgagg agttaaaatc atgctttcag gcttaatagt atgatttcta
      121 aacttaattt tttcatctgt acgttcgcta tataatgctt catcagaacg ataagacgct
      181 gacgcaacga tattttttgt tgctcgatta atgttagtca ctctcaacat aaagtaacct
      241 ttattcatga ttttacctcc tctcttttct gtttttttac ccctgtggga actataatga
      301 tcaaccagta tggttgccca tttttatgcc aattttcaat ttgcatgtaa atgggcagtg
      361 cagtgtctta agaactggtt atattatacc atggatgtgc gacttaccca atgaattatg
      421 gtatgcttta tatagataaa ctaagggagt tttttaatgt ctaaaattaa acaaacagat
      481 attacaaaat taagtcaatt agaaaaacaa attgcacaac tgaaagagca aaagaaaaaa
      541 caagaagaac aactatctaa taaaattggt ggttattttt taaataaact tgatttaaat
      601 atgatcaact ctagtgatga attgtataaa attatggata aagtcattgg agaatttaat
      661 aaagatatcg aacaaaatag cagcaacgag aacaaggaaa atttaacaga agacaaaatt
      721 aactagaata gtaagtgata cgatgaatga ttttaaacat ctcaaaaaaa taaatgctga
      781 aattgaaaaa gcagaattaa gaaagcatag attaaaaaat ttagatagaa aagaaagagc
      841 acatagactg attcgaaaag gtgcaatgct tgaaaagtat tttgaatgtg agcatttaag
      901 tcctgatgaa acagaagaat tgttaaagat gtattccaac tacattaata caaataaacc
      961 caataaatat aaaaagaagt aatttatcat gcttacaaat acttgggttt cactaaaaag
     1021 aaaaaattgc tgatagtagt aacaatgaat aataattgtt tataaaaatg ggactaaaaa
     1081 agaaaagttt tagtcccact tacttaatta attacatttc attgaaaata aagttcgctt
     1141 acagttaaga gaacatctaa ttaaagatat tctctaattg gatcattgtt ggcttattaa
     1201 gttgtattat tgattaaata aagaagtgat tggtaaatga tcacgtcgtt tattaagcgt
     1261 atgtacttct gcaaattcag atttaagaga tttcattaag tacccttgca ttgcttcaac
     1321 ggtttcattt ttttgaatta atgtagcttt aaaacgctta agaacattaa caatttcaaa
     1381 ttccatatct tctaataaat aatatgcatc aatagaattg ttgaaggatg ttttggcttt
     1441 taataacaca gatttaataa gtttaatttc agatacagaa aagttactta aataactttg
     1501 aatactttgt ggtagttctt caagtaattg aaattttaaa gcttcatcat tgaattttga
     1561 atagtagtga tttgaatgat ttgtatgatt actagaatat gttaatttcc tatcattcaa
     1621 atcatacata tcattataat tagtcttaat ataatcagtg tcattataat cagtatcatt
     1681 agactgtgaa tttggaagtt cttgaccact taaattcaca gttctagaac ttccaaattc
     1741 attgttctgg aactgtgaat ttggaagttc cttgatatga ctggcttcaa tgtcataatt
     1801 aggttttaat aggtataatc tatttggttt attaaggcca acacgttctt gagttagtaa
     1861 attaaatttt attaactctt tttttaattt tataattttt tgttctgcat aacctaagta
     1921 ttcacacaaa tgtttattac tgaaaataaa gtaaatatta ttatttttat ctacccattc
     1981 attttggagg gataattcaa aacgatcttt taataacgca tacataattt tggcttctgc
     2041 agacattttc ttaaaatatg catcttcaaa taaatattta ggcagttgat aaaatctttc
     2101 agtttttact tgtgataaat tatatttttc aaaattgggc ataataaaaa cctcatttcc
     2161 attttgggat tgaggtaata aaacaatttt tacgtaagtt atatgttttg tgcttgcact
     2221 tagatgatta gatatatata atacaagtat caaaaaaata tataaacaaa cgagtgcgaa
     2281 tcgttttatt accgagagtc gatgctacgc caatagcatc gactctttta atatttataa
     2341 agtaatatta acacttttaa tggcgtatat aaagagtttt aaatgtaatt aaaagtacac
     2401 ctaaaactac caaacgtata attcgctatt ttagcaccta attaagtggc gaaaatattt
     2461 taaggataga taattaggca tttaggtagt taatagatag taaatttagt actaaatgat
     2521 gaaataaatt ttgttaggtg gtaaaattta ggtactaaat taggtaccta aggggggatg
     2581 ttatgaaaac tattaaaatg attgctgatg aattaggtgt aactaaacaa actattgtta
     2641 ataatgctaa aagattaaat atatcttttg aaaaagaaaa tggaattaat tatattaaag
     2701 ataatgatta tttaaaaatt atagaaaaaa tcactaagaa agagaaaaca actcataata
     2761 aagaatcagt aaaaaatgaa ttatttaatg aaaatactgc aaaagttgaa aagtatatac
     2821 ataataattc aaatagttct gagacattaa aaacaaaagt aaacgaatta gaaaagcaaa
     2881 tagaaatctt tgaaactaga gctaaaaatg atgaaaaata tattgagaat ctaactaaac
     2941 aattagatca acaaaatagc aatgttaata cattaaataa actgttggag aatcaacaaa
     3001 tattatcttt agaaaacaat aaaaaaattc aaaaactaga aagccaatta gaagaagaaa
     3061 gacagcttaa atattctttt gatacaacga tgaataatag acagaatatt aatccacaag
     3121 aagcaacttt tactgaagaa tctcaaaata tcaaccaaca acaaaaagaa tgtcaaccaa
     3181 ccgaagtaaa tcataaaaat ataactgaag agaataagca tgacagtaca gatgaattaa
     3241 caaaagagga taagtttgtt gaaaaaacga agaacgattc cagtgataat agggaggtag
     3301 aaccacctaa aaagagattt tggagtcgtt tatttggtaa ctaattgcaa ttaaaaaggc
     3361 acacaagttc atgaacttgt tgtgcctttt taatgtgaat caaacttctg ataatcatca
     3421 gttatgttaa actctaacaa aaactaaatt atcagaagaa ttatctaggg tattaaacat
     3481 aggtaaatca attttctatc gccaatagta taaacatcat ccatattaat ctgaagatag
     3541 ctacataatt ctttaacagc atagccttta tttgtataag gcgccataaa ctctattaaa
     3601 tatggtttgt tttgaggttt ctatgtttaa tcatttaatt tttcattata taaatcgaat
     3661 aattataaaa aatcatcata ctttttattt tttaaaaata tttgaaaatt taatagtaat
     3721 ccaattataa gatcaaatat cattatcatt ataatcaagg caactataat cactgtgaca
     3781 ctcgttacat aacaagctag taatctccag ataaaattga cattttcacc aattccataa
     3841 cttaacatat tacaaagtag taaaagaata ataataccta tactgtaaat taaaattgtc
     3901 agtgtatatt ttttaataac tctattagga aacaatagtt catctattag gttctttttc
     3961 accagtatct ctcgttcaat tctatctttg tcacagattt tgattttact attttttatt
     4021 ttttcctcta ataaaataac ctcgaacgat ctgctaattt ttaataaata tgcaggaaaa
     4081 gctaatgtta agcataatgt aattaattca tttacattgt tcatgtaact tataaaatag
     4141 atgaaagttg ctactagtgc tcctaaaaat atagttatat taagttctac atcaaatatt
     4201 ttaaaaatat ctgctctatt catcagttaa tcatcctttc aaggtttatt aatactaata
     4261 aattattaga tataggtata tcatattatt aatttaagaa attgtctttt ataattttac
     4321 ttaataataa aaaagtagaa ccatttaaaa ttaatggttc tactttttaa ctattactaa
     4381 ttttaaaaat aaactaatct acaatatcta aaaatatatg tttagtacta tttttgttta
     4441 gttatatcaa gtgatttggg aattaacaaa tagacaacta ttgaaatgat aatcatgata
     4501 atcccaccta ctaaagcagt tgctacaaaa gcatcattaa atgatgttac agcctcgttt
     4561 gcaagctgtt ttatccccgt agctttagct acttcgacag cgcccactac agattcttca
     4621 gctacatgag ctaaatctcc aactatacct tttgatgaaa aagatgaaat atctaaaaat
     4681 acacgataaa gcatagaaga taggctacca agtactgcta caccaaaaac atttccaagg
     4741 tcatacatag actcttcaac agcagctgca ttacctgctt ttgatgtagg tgtttctaac
     4801 attattagag cagatgcaac tgctagtgaa gccataccag ctccaactaa aattaatgct
     4861 aaagccattg tagaatatga taatggatga ccaaagaaat acataataaa catgccaatg
     4921 gctgcaattc caattccgga aggtaacact attttcggtc caaatcgcgc cgctaatccg
     4981 ggtgcaattg gtgcaaacac catatctcct attgccatag gtaataggta taagccagct
     5041 ttaaaaggag aaagttcttc cacaacctgt aaccattgtg aagctaataa caaaacagat
     5101 gccattgcaa aaattgtcat aaatgcagca attgtaccag ctgaaaatga tctcttttta
     5161 aaaagtctta cgtctaacat tggatcagaa cttgataaat tacgtttaac aaatatcact
     5221 atcatggtaa ttgctaatac tattacaacc catggaataa tatctgctag tccttctttt
     5281 gaaaattctt tgatactcca taccagtcca atcatgcctg caattgataa aattgtagaa
     5341 ggaatatccc acgagtgaga cttttctttt gataacttag actctggtaa taaaaataaa
     5401 cctgctacaa ctgctattat cgcaaacggt acattaatta aaaatgccga gtgccatgaa
     5461 aattgctcaa gtaaagctcc tccgataatt ggtccaaaaa cagcacctat cgatgaagcg
     5521 attgaccata cagctaatgc agtggccctt tctttagggt tttcaaaaat tactcttatc
     5581 attgaaagag tagttggcat tattaaagca cctgcaatac caagtaaaaa tcgaatagct
     5641 attacgaact ctgcactttc tgcgaaaaat atagctaatg aaacgaggcc aaataaagca
     5701 aatccagtta ataatgcttt ttttcttccc catttatcag caaaggcact caatggaatt
     5761 ataaagccag ctaaaacaag agagtatata tcaactatcc ataactgttg ggtaccagaa
     5821 ggctctaact ctcttactaa ttccggtaaa gccataatta atattgtcat atccattgta
     5881 acaacaaaca aactaacagc taatactact agtgcagtcc atcttttttt tgatgtcatc
     5941 atatcagtag tttttgtaaa aaatgaaatc atttaagttc cctccaatcc ttatagaccg
     6001 tgcgatcggt ctataaggat tataatctat tttttttaca tattacaaca aaaaatcttg
     6061 cgaaatttaa atggctgtat tgtgttatac aattattctt tgaatttttt gtgctatcat
     6121 tgatagtact taatactcat taaaggcgtg atgaacttga aagataaaat actaggtgtc
     6181 gcaaaggaat tatttataaa aaatggatat aatgccacta ctactggaga aattgttaaa
     6241 ttatcagaaa gtagtaaagg gaatctttat tatcacttta aaacaaaaga aaatctattt
     6301 ttagaaattt taaatataga agaatctaaa tggcaagaac agtggaaaaa ggaacaaatc
     6361 aaatgtaaaa ctaatagaga aaaattttat ttatataatg aactatcttt aacaaccgaa
     6421 tactattatc cacttcaaaa cgcaataatt gaattttaca ctgaatatta taaaactaat
     6481 agcattaatg aaaaaatgaa taaattagaa aacaaatata tagatgctta tcatgtaatt
     6541 tttaaggagg gtaatttaaa tggcgaatgg tgtattaatg atgttaatgc tgttagtaaa
     6601 atagcagcaa acgctgttaa tggaattgtt acctttacac atgaacaaaa tattaatgaa
     6661 agaattaaac ttatgaacaa gttctctcaa atttttttaa atggacttag taaataatta
     6721 tatatatatt cttgaaatac caataaatat tatttctaaa ttacgttcgt ttttgacaca
     6781 aaacctctta acttaatatt aagaggtttt tcttggatat ttaagtctat aaacttgtgt
     6841 tataatcaat gtatcataaa tatcgaaaat catgagtttt tagatgctga cagacgtgtt
     6901 tatttacatc gagaactata tttcattagt aagtacccat aaaacgatta aaaaaggtgg
     6961 tgttcaaatg gctaaaattg gttatgcacg tgtgtcaaca caagatcaaa gtcttgatgg
     7021 acagattgat acacttgagg aatatggttg tgaacgtatc tttagcgaga aagcgagtgg
     7081 tcgtaaaact aaaagaacgg aacttgataa gtgtttagat tatttacgtg aaggagatat
     7141 tctagttgtc tataaattag atcgtcttgg acgtacaaca aaacaattaa ttgagctatc
     7201 tcaatggttg gatgaaaata gcattgattt acatattata gatatgaatg tgtcaacgaa
     7261 agatgcgatg ggcaagatgt tctttaccat gatgagtgca ttcgctgaac ttgaagctaa
     7321 tttattaagt gaacgaacta aaaaaggttt ggaagctgca agagcaagag ggagaaaagg
     7381 tggaagacct tcactaccag atcataagaa aagagagatc aaattcttat atgatgaaca
     7441 aaagctgtct ggtgaagaaa ttgctgaaca aacaggagtg agtcgttcta ctgtatatag
     7501 gattattaaa gagtcaagaa aaatataaag tactaaatta aagttttaat atacccttta
     7561 attgaaaaat cttcttaaaa agtgtcttta acttcagtcc cctttaacaa tccactcgct
     7621 taatagcttt aaatttctaa gcattacttg agctttgaaa attttcatcg attcattaac
     7681 cgacataact ggattttgac tgttaatatt caccttgtcc tctccaatta acaaaaaagc
     7741 aaaagcatat aaacaacatt tgttgtttat atgcttttgc ttgcattttc ttctatagta
     7801 tgctataatg aaaaggttaa gtctatctat acatcaaaag caataaactt ccacctagaa
     7861 taacaactta ttgtaaatcg acaattacaa taatacatca ttctatattg gaagtaaaca
     7921 aaaaacttac agaaggatga tatagagtga cactagtatg taccaaattc aatcatgatg
     7981 ctaaatcaac tgattattta aatatttcta ttgaagaaga cactcttcaa tatatagacc
     8041 caaaccttat taaagtaata tctaatagag aagttggaaa agttgcgact ttggctcaaa
     8101 gagcacaaga gcaaattgat gttttttttg aaaaaattat cgatatacat cttagtgatt
     8161 tttctgatac ccaaaagcgt aatgaattta gaaaattatt ctctcatttt tcagaaccac
     8221 accatttacg cttaggtttt tctcagaaag gtaatacagg aaaaggtact acagcatctg
     8281 aattgattaa tctatttatg aatgaagata ttctttccat tatcttaaat gatgacggat
     8341 taacaatacc tcaaaaagca ccattaatca gaaattttgg tgatgataaa ttatcagatt
     8401 taacaagtaa tataatcatg aatgttataa ttgattttaa taaatccatg attcagtcat
     8461 ttccagaaat gaaatcgttt ctttctaaag aaactgtgac atattattat ttctctacta
     8521 acggtatatg ggaaacttat gatttcactc catttctttt tgatggtaat aaaactttat
     8581 tagttccaaa attattcact acttataatc agacatctag tttagatttg atgataagag
     8641 tatacattga agaagaaatc attagattgc aaaaacaacg taatacaacc aaaaaaatga
     8701 caaaaaaaca atttatcaat gaatttataa aaggacatcg tatggataat atgacctatt
     8761 tctttttaaa cacttcttca aaatcacaca aaaaatttca aaaagaatta gattcaaaag
     8821 ccaatgaaag gggtaacaat aacaaagatg ataattagta cttactaatc agtatagtat
     8881 agacttaaca tactaattat tcaattttgc attcctccac actatgatgt tataatttag
     8941 ataacaaata tgtgttgaac atggtaaaca ccgagcccga taactattcc agtagttatc
     9001 gggctctttt tggtgttatt actgtgtcgt cgctttattg tttatcacga ttattcaacc
     9061 aatgtgaaaa caacgtgatt ataaccccta ctataatagg agctactata gatacaaata
     9121 tgatgctgaa catggtgtca cctccttcca tgctaaacca gcacagaagt agaacgacga
     9181 cttttatatt atatcatttg tgaattgtta agcatatgca cttccgataa gtcattttat
     9241 gtttaatgta tctatgtgat aatgaaaata caaattctat taaaggagat aattttatga
     9301 aaaaacgatc attacttcca actttattat tggctagctc attaacattc acaggttttt
     9361 cagcgacagc caatgctgag gatggtgcca aaaccgtata cggagagaac ttaccaaata
     9421 gcgcagatac aaatggagat ggttgggcta atgtagactt caatacttca tccctcccac
     9481 aatctgcaca aaatcaaata agtgaacttt ctaaacagaa agattcaggg gaattaactc
     9541 aaatcgagta taatgagaaa gtttcaagtg tatttaatga atatcaatca caaagtactg
     9601 atatacaaaa taatgagaga actcaacaag gtaaaagacc tttcggaggg gtgataccta
     9661 attgtatgac tcaagaagaa taccaagaat tagaaagtca tgttcctaac cctaatagtg
     9721 tatccacaga aaaatataat caaattgtaa aaaatgaaag tcaaaagata gctaatgaag
     9781 aaaataaaac tattaataca cctcaaggtg tgttgacacc tgtaactgaa aataaaaaca
     9841 agagttctga aggaatgaaa aatcaagatt caaactctca agtattacca gaaacaggag
     9901 aaagcaacca aagtgatata ttgtcaataa ttggcgcttc tggactacta atattaggta
     9961 taagtctttt aatattcaga aaaaaggcaa tataaaaatt gataaaataa ccaccgtgtt
    10021 tatgtaaaac atgtggttat tttattatat atttaataca tatttttcaa ctcatgtaat
    10081 ttacgttttc ttgctgaaaa aatattaaca taatctaatt tgaaatactg aaaaggagtt
    10141 gtaatatgaa aaaaatcatt ttacctatat taattgttag tttatcggta attttagtga
    10201 gttgtaataa taacgattca gaacatcaca atgataataa ttctagtgat actaacacct
    10261 cagaaaacaa agataatgaa gcatcaaatg aaattgacga aaaaactaac acatctgata
    10321 gtgcggataa ttcagaaagt agtaataaac aaaataaaga aaaaaatgat aataaaaaac
    10381 aagaaaaaaa taatgatagt aataagaagc aacaatctac tgataaaaat aaaagttact
    10441 taaataattt taactcgaaa gaaattgaat atgcacgagt ttggtatcag ttaatatcta
    10501 caagaaatga tttaaaaggt attaaaaatg tttatgttac agaaatacca aaaggctcaa
    10561 aagtaaatcc acaagccaaa aatagtgcaa tttataaaga aaaagttgtg aaattagaag
    10621 ctcctatgag agctggtggg tctgtaacat atagcagtaa tggtgatgga acaattaatg
    10681 tatataataa tatcccttat aaatgggaaa attcacaaaa tagtgattat agtcaaatga
    10741 ataagataac aaaaaaagca atagaggaga atattgaaac agtctatatt aaaccctacg
    10801 acaataaaac agtagctaag ttagcaagta aaattagata taataagtag ttagtagatt
    10861 attaaaaagg cgcctaaaat taggcgtctt ttttatcatt agttcaaaag ataagcataa
    10921 cgttccatgt aacagcatat aaagtaatga atcaattcat cactatctaa tgccaccatt
    10981 gttagttcag ccttgcatgg tttaacgtat ctcacataat ggtacaaatc ttcaaatagt
    11041 gcctgttttt cgggcatttt aaacaccttg tgtacattaa aacctatttc ttctaatcgc
    11101 tttgtaatca cgccgaaaac gaatgtgttc atgtgaaatc actccatcat ttttaatatt
    11161 cacatgtaca ctaaaaaaaa tccgatacat cggcttttta tttttggttt aatttatttc
    11221 tatattgttt tcactatcgc tcggacgcat aagtgttaaa acaccttcta ccatacctat
    11281 cacacccggt attcccgtcc aacaaaatag taaataaaga agagcatttt tatttttctt
    11341 aacataaaac ttatgagccc cgaaacctcc tagaaaaaat gctaatgcag agtaaacata
    11401 tttattaact tgcatgattt ccactccttt aattaattga tttagagaaa cattaaataa
    11461 tttagtgatt tctaaaattg tttttaagtc gggattactt acttctcttt cccaattaga
    11521 aatagaatgt ctcgataccc ctaacttttc agctaatttt tcttgagaca tgtttttact
    11581 ttttcttaac ttttgtattt gatgtccaat ttttgtttcc aaattatttt tctccttaac
    11641 tttaagatat atttaaactc aattttttct atcgatattt atgaacatta gtatttttta
    11701 tgctgaaata tgtgaacaat cgcataatat caaggttttt ctataattat acacgtttta
    11761 aaatacattg tgataataca tagaatgtac cttatctaag tatataaatg ttttctttaa
    11821 accaaagcta atttaccaca ttgggttaag tttcttatta ttaaagaatg aactttgtat
    11881 gatttcaact gcttactatc ttctcatttg gttatttctc tcttgtttct tcttcttttt
    11941 ctgatgatta atattgtttt gcttttcagt ttcgttcgca tagtacataa gaaagtcact
    12001 agcatttatc gttggtaaat taatgtgatt agtttgttca ttttcatgtt caatacgatt
    12061 gtcatgatca tttctatcta cttgattaat atgtttttcg ataacttctt cttgatcgac
    12121 atgattatat ttctcaagtt taatgagtaa ttgaactttt tcattaggtg tttggtcttt
    12181 gactttttca aagtttttat cgcctatatc tattcttaaa tcatcatcaa tatgttcttt
    12241 ataactatca atttcttttt ctttttcctt aataaattgc aactcaacgt tatattcaaa
    12301 gctatctttt tctaaatttt taagatgttt ttgactaaag aaaatatctt tactcgcttt
    12361 tttataagta tcttctagca atacatcatt aggaatattg ttattttgtt tgaacaggta
    12421 ttgaacatga gttgctttta taatattacc tttttgtgcc tcatcaacaa taaatgcttt
    12481 ttcatcatgg ctaaaatgat gatgagattt agggtacatt tgctgaacaa tgagatgatt
    12541 tttttcactt tcagttagat aaacatcttt agctgattga taaatctctt tacgtttttc
    12601 ctctctactt tctaatttat tcttctcatt gagtaacgct ttatagttag tttcaacagt
    12661 attaacaaac tcttgttgac tatatccttt actttcaatt tcttttctaa actgataatt
    12721 ttcatcataa tttttagcat agaattttaa ttgacgtctc ttaatttcat ttttaagtaa
    12781 atcattttga attttacgac caaaggtaga cgcactgtca aacgtatttt taattgtttc
    12841 tttcgcctct gaatagccaa catcacgttg atatctatca aataaaatag aataactttg
    12901 ttcattttta cttacttgtt cgttagcatt ttgaataaat aatctaattt cattttgtgc
    12961 ttgttctttc tctttatcac taaataattg tgatacttcg ccacgattat atcttctaat
    13021 ttgttcattt ttctgtccgt aataagtaac gggttggtac tcgataccct ctttttcaca
    13081 gcgttttctt tcattttgtt ctaatctata ttcttctctt gtaagtggaa tttcggcaat
    13141 tttttctaaa ccttgcgctt caaaagaacg actatcatat cgtcttaaac tttgttcacg
    13201 ttctgagtat tgatttaatt ttaattccca atgcttacga acttctttga aatcaaagtc
    13261 ggcaaatcgt tcttgttttc taactttatt accattttta tttaaaatcg cattgccatt
    13321 ttcatcatat tgcgttctcg tttttgtctt tttcccccaa gtaccgtctg cattaaacgg
    13381 tctttgagta agtagaacat gggcgtgtgg gttgtttata tcatctctat gaattgaaat
    13441 atcagcaacc attcctttgt ctacaaactc actttttata aaatctttag ttaattcagt
    13501 ttgtacctct ctatcaaaat cattaggcaa acttaataag acttcttttg ctaatctagg
    13561 attttttgtt
//
