LOCUS       NZ_CP073843            13570 bp    DNA     circular CON 17-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1264787 plasmid p2B1264787,
            complete sequence.
ACCESSION   NZ_CP073843
VERSION     NZ_CP073843.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699878
            Assembly: GCF_019329545.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 257x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019329545.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 06:57:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,613
            Genes (coding)                    :: 2,534
            CDSs (with protein)               :: 2,534
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 41 of 79
            Pseudo Genes (incomplete)         :: 44 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 24 of 79
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073843.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1264787"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p2B1264787"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(8..277)
                     /locus_tag="KFV40_RS13285"
                     /old_locus_tag="KFV40_13295"
     CDS             complement(8..277)
                     /locus_tag="KFV40_RS13285"
                     /old_locus_tag="KFV40_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            486..2408
                     /locus_tag="KFV40_RS13290"
                     /old_locus_tag="KFV40_13300"
     CDS             486..2408
                     /locus_tag="KFV40_RS13290"
                     /old_locus_tag="KFV40_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            2682..3095
                     /locus_tag="KFV40_RS13600"
                     /old_locus_tag="KFV40_13305"
     CDS             2682..3095
                     /locus_tag="KFV40_RS13600"
                     /old_locus_tag="KFV40_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            3172..3405
                     /locus_tag="KFV40_RS13300"
                     /old_locus_tag="KFV40_13310"
     CDS             3172..3405
                     /locus_tag="KFV40_RS13300"
                     /old_locus_tag="KFV40_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(3454..4158)
                     /locus_tag="KFV40_RS13305"
                     /old_locus_tag="KFV40_13315"
     CDS             complement(3454..4158)
                     /locus_tag="KFV40_RS13305"
                     /old_locus_tag="KFV40_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(4309..5007)
                     /locus_tag="KFV40_RS13310"
                     /old_locus_tag="KFV40_13320"
     CDS             complement(4309..5007)
                     /locus_tag="KFV40_RS13310"
                     /old_locus_tag="KFV40_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            5171..5269
                     /locus_tag="KFV40_RS13315"
                     /old_locus_tag="KFV40_13325"
     CDS             5171..5269
                     /locus_tag="KFV40_RS13315"
                     /old_locus_tag="KFV40_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(5446..6357)
                     /locus_tag="KFV40_RS13320"
                     /old_locus_tag="KFV40_13330"
     CDS             complement(5446..6357)
                     /locus_tag="KFV40_RS13320"
                     /old_locus_tag="KFV40_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(6776..7336)
                     /locus_tag="KFV40_RS13325"
                     /old_locus_tag="KFV40_13335"
     CDS             complement(6776..7336)
                     /locus_tag="KFV40_RS13325"
                     /old_locus_tag="KFV40_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            complement(7587..8153)
                     /gene="qacR"
                     /locus_tag="KFV40_RS13330"
                     /old_locus_tag="KFV40_13340"
     ARG             complement(7591..8153)
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            8332..9876
                     /gene="qac"
                     /locus_tag="KFV40_RS13335"
                     /old_locus_tag="KFV40_13345"
     CDS             8332..9876
                     /gene="qac"
                     /locus_tag="KFV40_RS13335"
                     /old_locus_tag="KFV40_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            10081..10641
                     /locus_tag="KFV40_RS13340"
                     /old_locus_tag="KFV40_13350"
     CDS             10081..10641
                     /locus_tag="KFV40_RS13340"
                     /old_locus_tag="KFV40_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(10960..11721)
                     /locus_tag="KFV40_RS13350"
                     /old_locus_tag="KFV40_13360"
     CDS             complement(10960..11721)
                     /locus_tag="KFV40_RS13350"
                     /old_locus_tag="KFV40_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            12162..13097
                     /locus_tag="KFV40_RS13355"
                     /old_locus_tag="KFV40_13365"
     CDS             12162..13097
                     /locus_tag="KFV40_RS13355"
                     /old_locus_tag="KFV40_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            complement(13322..13561)
                     /locus_tag="KFV40_RS13360"
                     /old_locus_tag="KFV40_13370"
     CDS             complement(13322..13561)
                     /locus_tag="KFV40_RS13360"
                     /old_locus_tag="KFV40_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     ARG             8333..9873
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          12163..13097
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          487..2339
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 tactattcta gttaattttg tcttctgtta aattttcctt gttctcgttg ctgctatttt
       61 gttcgatatc tttattaaat tctccaatga ctttatccat aattttatac aattcatcac
      121 tagagttgat catatttaaa tcaagtttat ttaaaaaata accaccaatt ttattagata
      181 gttgttcttc ttgttttttc ttttgctctt tcagttgtgc aatttgtttt tctaattgac
      241 ttaattttgt aatatctgtt tgtttaattt tagacattaa aaaactccct tagtttatct
      301 atataaagca taccataatt cattgggtaa gtcgcacatc catggtataa tataaccagt
      361 tcttaagaca ctgcactgcc catttacatg caaattgaaa attggcataa aaatgggcaa
      421 ccatactggt tgatcattat agttcccaca ggggtaaaaa aacagaaaag agaggaggta
      481 aaatcatgaa taaaggttac tttatgttga gagtgactaa cattaatcga gcaacaaaaa
      541 atatcgttgc gtcagcgtct tatcgttctg atgaagcatt atatagcgaa cgtacagatg
      601 aaaaaattaa gtttagaaat catactatta agcctgaaag catgatttta actcctcaac
      661 atgcacccga atggactaaa gatagacagc gtttatggaa tgaagttgat aaagttgaaa
      721 agaataatgc gaaaacaaaa aatcctagat tagcaaaaga agtcttatta agtttgccta
      781 atgattttga tagagaggta caaactgaat taactaaaga ttttataaaa agtgagtttg
      841 tagacaaagg aatggttgct gatatttcaa ttcatagaga tgatataaac aacccacacg
      901 cccatgttct acttactcaa agaccgttta atgcagacgg tacttggggg aaaaagacaa
      961 aaacgagaac gcaatatgat gaaaatggca atgcgatttt aaataaaaat ggtaataaag
     1021 ttagaaaaca agaacgattt gccgactttg atttcaaaga agttcgtaag cattgggaat
     1081 taaaattaaa tcaatactca gaacgtgaac aaagtttaag acgatatgat agtcgttctt
     1141 ttgaagcgca aggtttagaa aaaattgccg aaattccact tacaagagaa gaatatagat
     1201 tagaacaaaa tgaaagaaaa cgctgtgaaa aagagggtat cgagtaccaa cccgttactt
     1261 attacggaca gaaaaatgaa caaattagaa gatataatcg tggcgaagta tcacaattat
     1321 ttagtgataa agagaaagaa caagcacaaa atgaaattag attatttatt caaaatgcta
     1381 acgaacaagt aagtaaaaat gaacaaagtt attctatttt atttgataga tatcaacgtg
     1441 atgttggcta ttcagaggcg aaagaaacaa ttaaaaatac gtttgacagt gcgtctacct
     1501 ttggtcgtaa aattcaaaat gatttactta aaaatgaaat taagagacgt caattaaaat
     1561 tctatgctaa aaattatgat gaaaattatc agtttagaaa agaaattgaa agtaaaggat
     1621 atagtcaaca agagtttgtt aatactgttg aaactaacta taaagcgtta ctcaatgaga
     1681 agaataaatt agaaagtaga gaggaaaaac gtaaagagat ttatcaatca gctaaagatg
     1741 tttatctaac tgaaagtgaa aaaaatcatc tcattgttca gcaaatgtac cctaaatctc
     1801 atcatcattt tagccatgat gaaaaagcat ttattgttga tgaggcacaa aaaggtaata
     1861 ttataaaagc aactcatgtt caatacctgt tcaaacaaaa taacaatatt cctaatgatg
     1921 tattgctaga agatacttat aaaaaagcga gtaaagatat tttctttagt caaaaacatc
     1981 ttaaaaattt agaaaaagat agctttgaat ataacgttga gttgcaattt attaaggaaa
     2041 aagaaaaaga aattgatagt tataaagaac atattgatga tgatttaaga atagatatag
     2101 gcgataaaaa ctttgaaaaa gtcaaagacc aaacacctaa tgaaaaagtt caattactca
     2161 ttaaacttga gaaatataat catgtcgatc aagaagaagt tatcgaaaaa catattaatc
     2221 aagtagatag aaatgatcat gacaatcgta ttgaacatga aaatgaacaa actaatcaca
     2281 ttaatttacc aacgataaat gctagtgact ttcttatgta ctatgcgaac gaaactgaaa
     2341 agcaaaacaa tattaatcat cagaaaaaga agaagaaaca agagagaaat aaccaaatga
     2401 gaagatagta agcagttgaa atcatacaaa gttcattctt taataataag aaacttaacc
     2461 caatgtggta aattagcttt ggtttaaaga aaacatttat atacttagat aaggtacatt
     2521 ctatgtatta tcacaatgta ttttaaaacg tgtataatta tagaaaaacc ttgatattat
     2581 gcgattgttc acatatttca gcataaaaaa tactaatgtt cataaatatc gatagaaaaa
     2641 attgagttta aatatatctt aaagttaagg agaaaaataa tttggaaaca aaaattggac
     2701 atcaaataca aaagttaaga aaaagtaaaa acatgtctca agaaaaatta gctgaaaagt
     2761 taggggtatc gagacattct atttctaatt gggaaagaga agtaagtaat cccgacttaa
     2821 aaacaatttt agaaatcact aaattattta atgtttctct aaatcaatta attaaaggag
     2881 tggaaatcat gcaagttaat aaatatgttt actctgcatt agcatttttt ctaggaggtt
     2941 tcggggctca taagttttat gttaagaaaa ataaaaatgc tcttctttat ttactatttt
     3001 gttggacggg aataccgggt gtgataggta tggtagaagg tgttttaaca cttatgcgtc
     3061 cgagcgatag tgaaaacaat atagaaataa attaaaccaa aaataaaaag ccgatgtatc
     3121 ggattttttt tagtgtacat gtgaatatta aaaatgatgg agtgatttca catgaacaca
     3181 ttcgttttcg gcgtgattac aaagcgatta gaagaaatag gttttaatgt acacaaggtg
     3241 tttaaaatgc ccgaaaaaca ggcactattt gaagatttgt accattatgt gagatacgtt
     3301 aaaccatgca aggctgaact aacaatggtg gcattagata gtgatgaatt gattcattac
     3361 tttatatgct gttacatgga acgttatgct tatcttttga actaatgata aaaaagacgc
     3421 ctaattttag gcgccttttt aataatctac taactactta ttatatctaa ttttacttgc
     3481 taacttagct actgttttat tgtcgtaggg tttaatatag actgtttcaa tattctcctc
     3541 tattgctttt tttgttatct tattcatttg actataatca ctattttgtg aattttccca
     3601 tttataaggg atattattat atacattaat tgttccatca ccattactgc tatatgttac
     3661 agacccacca gctctcatag gagcttctaa tttcacaact ttttctttat aaattgcact
     3721 atttttggct tgtggattta cttttgagcc ttttggtatt tctgtaacat aaacattttt
     3781 aatacctttt aaatcatttc ttgtagatat taactgatac caaactcgtg catattcaat
     3841 ttctttcgag ttaaaattat ttaagtaact tttattttta tcagtagatt gttgcttctt
     3901 attactatca ttattttttt cttgtttttt attatcattt ttttctttat tttgtttatt
     3961 actactttct gaattatccg cactatcaga tgtgttagtt ttttcgtcaa tttcatttga
     4021 tgcttcatta tctttgtttt ctgaggtgtt agtatcacta gaattattat cattgtgatg
     4081 ttctgaatcg ttattattac aactcactaa aattaccgat aaactaacaa ttaatatagg
     4141 taaaatgatt tttttcatat tacaactcct tttcagtatt tcaaattaga ttatgttaat
     4201 attttttcag caagaaaacg taaattacat gagttgaaaa atatgtatta aatatataat
     4261 aaaataacca catgttttac ataaacacgg tggttatttt atcaattttt atattgcctt
     4321 ttttctgaat attaaaagac ttatacctaa tattagtagt ccagaagcgc caattattga
     4381 caatatatca ctttggttgc tttctcctgt ttctggtaat acttgagagt ttgaatcttg
     4441 atttttcatt ccttcagaac tcttgttttt attttcagtt acaggtgtca acacaccttg
     4501 aggtgtatta atagttttat tttcttcatt agctatcttt tgactttcat tttttacaat
     4561 ttgattatat ttttctgtgg atacactatt agggttagga acatgacttt ctaattcttg
     4621 gtattcttct tgagtcatac aattaggtat cacccctccg aaaggtcttt taccttgttg
     4681 agttctctca ttattttgta tatcagtact ttgtgattga tattcattaa atacacttga
     4741 aactttctca ttatactcga tttgagttaa ttcccctgaa tctttctgtt tagaaagttc
     4801 acttatttga ttttgtgcag attgtgggag ggatgaagta ttgaagtcta cattagccca
     4861 accatctcca tttgtatctg cgctatttgg taagttctct ccgtatacgg ttttggcacc
     4921 atcctcagca ttggctgtcg ctgaaaaacc tgtgaatgtt aatgagctag ccaataataa
     4981 agttggaagt aatgatcgtt ttttcataaa attatctcct ttaatagaat ttgtattttc
     5041 attatcacat agatacatta aacataaaat gacttatcgg aagtgcatat gcttaacaat
     5101 tcacaaatga tataatataa aagtcgtcgt tctacttctg tgctggttta gcatggaagg
     5161 aggtgacacc atgttcagca tcatatttgt atctatagta gctcctatta tagtaggggt
     5221 tataatcacg ttgttttcac attggttgaa taatcgtgat aaacaataaa gcgacgacac
     5281 agtaataaca ccaaaaagag cccgataact actggaatag ttatcgggct cggtgtttac
     5341 catgttcaac acatatttgt tatctaaatt ataacatcat agtgtggagg aatgcaaaat
     5401 tgaataatta gtatgttaag tctatactat actgattagt aagtactaat tatcatcttt
     5461 gttattgtta cccctttcat tggcttttga atctaattct ttttgaaatt ttttgtgtga
     5521 ttttgaagaa gtgtttaaaa agaaataggt catattatcc atacgatgtc cttttataaa
     5581 ttcattgata aattgttttt ttgtcatttt tttggttgta ttacgttgtt tttgcaatct
     5641 aatgatttct tcttcaatgt atactcttat catcaaatct aaactagatg tctgattata
     5701 agtagtgaat aattttggaa ctaataaagt tttattacca tcaaaaagaa atggagtgaa
     5761 atcataagtt tcccatatac cgttagtaga gaaataataa tatgtcacag tttctttaga
     5821 aagaaacgat ttcatttctg gaaatgactg aatcatggat ttattaaaat caattataac
     5881 attcatgatt atattacttg ttaaatctga taatttatca tcaccaaaat ttctgattaa
     5941 tggtgctttt tgaggtattg ttaatccgtc atcatttaag ataatggaaa gaatatcttc
     6001 attcataaat agattaatca attcagatgc tgtagtacct tttcctgtat tacctttctg
     6061 agaaaaacct aagcgtaaat ggtgtggttc tgaaaaatga gagaataatt ttctaaattc
     6121 attacgcttt tgggtatcag aaaaatcact aagatgtata tcgataattt tttcaaaaaa
     6181 aacatcaatt tgctcttgtg ctctttgagc caaagtcgca acttttccaa cttctctatt
     6241 agatattact ttaataaggt ttgggtctat atattgaaga gtgtcttctt caatagaaat
     6301 atttaaataa tcagttgatt tagcatcatg attgaatttg gtacatacta gtgtcactct
     6361 atatcatcct tctgtaagtt ttttgtttac ttccaatata gaatgatgta ttattgtaat
     6421 tgtcgattta caataagttg ttattctagg tggaagttta ttgcttttga tgtatagata
     6481 gacttaacct tttcattata gcatactata gaagaaaatg caagcaaaag catataaaca
     6541 acaaatgttg tttatatgct tttgcttttt tgttaattgg agaggacaag gtgaatatta
     6601 acagtcaaaa tccagttatg tcggttaatg aatcgatgaa aattttcaaa gctcaagtaa
     6661 tgcttagaaa tttaaagcta ttaagcgagt ggattgttaa aggggactga agttaaagac
     6721 actttttaag aagatttttc aattaaaggg tatattaaaa ctttaattta gtactttata
     6781 tttttcttga ctctttaata atcctatata cagtagaacg actcactcct gtttgttcag
     6841 caatttcttc accagacagc ttttgttcat catataagaa tttgatctct cttttcttat
     6901 gatctggtag tgaaggtctt ccaccttttc tccctcttgc tcttgcagct tccaaacctt
     6961 ttttagttcg ttcacttaat aaattagctt caagttcagc gaatgcactc atcatggtaa
     7021 agaacatctt gcccatcgca tctttcgttg acacattcat atctataata tgtaaatcaa
     7081 tgctattttc atccaaccat tgagatagct caattaattg ttttgttgta cgtccaagac
     7141 gatctaattt atagacaact agaatatctc cttcacgtaa ataatctaaa cacttatcaa
     7201 gttccgttct tttagtttta cgaccactcg ctttctcgct aaagatacgt tcacaaccat
     7261 attcctcaag tgtatcaatc tgtccatcaa gactttgatc ttgtgttgac acacgtgcat
     7321 aaccaatttt agccatttga acaccacctt ttttaatcgt tttatgggta cttactaatg
     7381 aaatatagtt ctcgatgtaa ataaacacgt ctgtcagcat ctaaaaactc atgattttcg
     7441 atatttatga tacattgatt ataacacaag tttatagact taaatatcca agaaaaacct
     7501 cttaatatta agttaagagg ttttgtgtca aaaacgaacg taatttagaa ataatattta
     7561 ttggtatttc aagaatatat atataattat ttactaagtc catttaaaaa aatttgagag
     7621 aacttgttca taagtttaat tctttcatta atattttgtt catgtgtaaa ggtaacaatt
     7681 ccattaacag cgtttgctgc tattttacta acagcattaa catcattaat acaccattcg
     7741 ccatttaaat taccctcctt aaaaattaca tgataagcat ctatatattt gttttctaat
     7801 ttattcattt tttcattaat gctattagtt ttataatatt cagtgtaaaa ttcaattatt
     7861 gcgttttgaa gtggataata gtattcggtt gttaaagata gttcattata taaataaaat
     7921 ttttctctat tagttttaca tttgatttgt tcctttttcc actgttcttg ccatttagat
     7981 tcttctatat ttaaaatttc taaaaataga ttttcttttg ttttaaagtg ataataaaga
     8041 ttccctttac tactttctga taatttaaca atttctccag tagtagtggc attatatcca
     8101 ttttttataa ataattcctt tgcgacacct agtattttat ctttcaagtt catcacgcct
     8161 ttaatgagta ttaagtacta tcaatgatag cacaaaaaat tcaaagaata attgtataac
     8221 acaatacagc catttaaatt tcgcaagatt ttttgttgta atatgtaaaa aaaatagatt
     8281 ataatcctta tagaccgatc gcacggtcta taaggattgg agggaactta aatgatttca
     8341 ttttttacaa aaactactga tatgatgaca tcaaaaaaaa gatggactgc actagtagta
     8401 ttagctgtta gtttgtttgt tgttacaatg gatatgacaa tattaattat ggctttaccg
     8461 gaattagtaa gagagttaga gccttctggt acccaacagt tatggatagt tgatatatac
     8521 tctcttgttt tagctggctt tataattcca ttgagtgcct ttgctgataa atggggaaga
     8581 aaaaaagcat tattaactgg atttgcttta tttggcctcg tttcattagc tatatttttc
     8641 gcagaaagtg cagagttcgt aatagctatt cgatttttac ttggtattgc aggtgcttta
     8701 ataatgccaa ctactctttc aatgataaga gtaatttttg aaaaccctaa agaaagggcc
     8761 actgcattag ctgtatggtc aatcgcttca tcgataggtg ctgtttttgg accaattatc
     8821 ggaggagctt tacttgagca attttcatgg cactcggcat ttttaattaa tgtaccgttt
     8881 gcgataatag cagttgtagc aggtttattt ttattaccag agtctaagtt atcaaaagaa
     8941 aagtctcact cgtgggatat tccttctaca attttatcaa ttgcaggcat gattggactg
     9001 gtatggagta tcaaagaatt ttcaaaagaa ggactagcag atattattcc atgggttgta
     9061 atagtattag caattaccat gatagtgata tttgttaaac gtaatttatc aagttctgat
     9121 ccaatgttag acgtaagact ttttaaaaag agatcatttt cagctggtac aattgctgca
     9181 tttatgacaa tttttgcaat ggcatctgtt ttgttattag cttcacaatg gttacaggtt
     9241 gtggaagaac tttctccttt taaagctggc ttatacctat tacctatggc aataggagat
     9301 atggtgtttg caccaattgc acccggatta gcggcgcgat ttggaccgaa aatagtgtta
     9361 ccttccggaa ttggaattgc agccattggc atgtttatta tgtatttctt tggtcatcca
     9421 ttatcatatt ctacaatggc tttagcatta attttagttg gagctggtat ggcttcacta
     9481 gcagttgcat ctgctctaat aatgttagaa acacctacat caaaagcagg taatgcagct
     9541 gctgttgaag agtctatgta tgaccttgga aatgtttttg gtgtagcagt acttggtagc
     9601 ctatcttcta tgctttatcg tgtattttta gatatttcat ctttttcatc aaaaggtata
     9661 gttggagatt tagctcatgt agctgaagaa tctgtagtgg gcgctgtcga agtagctaaa
     9721 gctacgggga taaaacagct tgcaaacgag gctgtaacat catttaatga tgcttttgta
     9781 gcaactgctt tagtaggtgg gattatcatg attatcattt caatagttgt ctatttgtta
     9841 attcccaaat cacttgatat aactaaacaa aaatagtact aaacatatat ttttagatat
     9901 tgtagattag tttattttta aaattagtaa tagttaaaaa gtagaaccat taattttaaa
     9961 tggttctact tttttattat taagtaaaat tataaaagac aatttcttaa attaataata
    10021 tgatatacct atatctaata atttattagt attaataaac cttgaaagga tgattaactg
    10081 atgaatagag cagatatttt taaaatattt gatgtagaac ttaatataac tatattttta
    10141 ggagcactag tagcaacttt catctatttt ataagttaca tgaacaatgt aaatgaatta
    10201 attacattat gcttaacatt agcttttcct gcatatttat taaaaattag cagatcgttc
    10261 gaggttattt tattagagga aaaaataaaa aatagtaaaa tcaaaatctg tgacaaagat
    10321 agaattgaac gagagatact ggtgaaaaag aacctaatag atgaactatt gtttcctaat
    10381 agagttatta aaaaatatac actgacaatt ttaatttaca gtataggtat tattattctt
    10441 ttactacttt gtaatatgtt aagttatgga attggtgaaa atgtcaattt tatctggaga
    10501 ttactagctt gttatgtaac gagtgtcaca gtgattatag ttgccttgat tataatgata
    10561 atgatatttg atcttataat tggattacta ttaaattttc aaatattttt aaaaaataaa
    10621 aagtatgatg attttttata attattcgat ttatataatg aaaaattaaa tgattaaaca
    10681 tagaaacctc aaaacaaacc atatttaata gagtttatgg cgccttatac aaataaaggc
    10741 tatgctgtta aagaattatg tagctatctt cagattaata tggatgatgt ttatactatt
    10801 ggcgatagaa aattgattta cctatgttta ataccctaga taattcttct gataatttag
    10861 tttttgttag agtttaacat aactgatgat tatcagaagt ttgattcaca ttaaaaaggc
    10921 acaacaagtt catgaacttg tgtgcctttt taattgcaat tagttaccaa ataaacgact
    10981 ccaaaatctc tttttaggtg gttctacctc cctattatca ctggaatcgt tcttcgtttt
    11041 ttcaacaaac ttatcctctt ttgttaattc atctgtactg tcatgcttat tctcttcagt
    11101 tatattttta tgatttactt cggttggttg acattctttt tgttgttggt tgatattttg
    11161 agattcttca gtaaaagttg cttcttgtgg attaatattc tgtctattat tcatcgttgt
    11221 atcaaaagaa tatttaagct gtctttcttc ttctaattgg ctttctagtt tttgaatttt
    11281 tttattgttt tctaaagata atatttgttg attctccaac agtttattta atgtattaac
    11341 attgctattt tgttgatcta attgtttagt tagattctca atatattttt catcattttt
    11401 agctctagtt tcaaagattt ctatttgctt ttctaattcg tttacttttg tttttaatgt
    11461 ctcagaacta tttgaattat tatgtatata cttttcaact tttgcagtat tttcattaaa
    11521 taattcattt tttactgatt ctttattatg agttgttttc tctttcttag tgattttttc
    11581 tataattttt aaataatcat tatctttaat ataattaatt ccattttctt tttcaaaaga
    11641 tatatttaat cttttagcat tattaacaat agtttgttta gttacaccta attcatcagc
    11701 aatcatttta atagttttca taacatcccc ccttaggtac ctaatttagt acctaaattt
    11761 taccacctaa caaaatttat ttcatcattt agtactaaat ttactatcta ttaactacct
    11821 aaatgcctaa ttatctatcc ttaaaatatt ttcgccactt aattaggtgc taaaatagcg
    11881 aattatacgt ttggtagttt taggtgtact tttaattaca tttaaaactc tttatatacg
    11941 ccattaaaag tgttaatatt actttataaa tattaaaaga gtcgatgcta ttggcgtagc
    12001 atcgactctc ggtaataaaa cgattcgcac tcgtttgttt atatattttt ttgatacttg
    12061 tattatatat atctaatcat ctaagtgcaa gcacaaaaca tataacttac gtaaaaattg
    12121 ttttattacc tcaatcccaa aatggaaatg aggtttttat tatgcccaat tttgaaaaat
    12181 ataatttatc acaagtaaaa actgaaagat tttatcaact gcctaaatat ttatttgaag
    12241 atgcatattt taagaaaatg tctgcagaag ccaaaattat gtatgcgtta ttaaaagatc
    12301 gttttgaatt atccctccaa aatgaatggg tagataaaaa taataatatt tactttattt
    12361 tcagtaataa acatttgtgt gaatacttag gttatgcaga acaaaaaatt ataaaattaa
    12421 aaaaagagtt aataaaattt aatttactaa ctcaagaacg tgttggcctt aataaaccaa
    12481 atagattata cctattaaaa cctaattatg acattgaagc cagtcatatc aaggaacttc
    12541 caaattcaca gttccagaac aatgaatttg gaagttctag aactgtgaat ttaagtggtc
    12601 aagaacttcc aaattcacag tctaatgata ctgattataa tgacactgat tatattaaga
    12661 ctaattataa tgatatgtat gatttgaatg ataggaaatt aacatattct agtaatcata
    12721 caaatcattc aaatcactac tattcaaaat tcaatgatga agctttaaaa tttcaattac
    12781 ttgaagaact accacaaagt attcaaagtt atttaagtaa cttttctgta tctgaaatta
    12841 aacttattaa atctgtgtta ttaaaagcca aaacatcctt caacaattct attgatgcat
    12901 attatttatt agaagatatg gaatttgaaa ttgttaatgt tcttaagcgt tttaaagcta
    12961 cattaattca aaaaaatgaa accgttgaag caatgcaagg gtacttaatg aaatctctta
    13021 aatctgaatt tgcagaagta catacgctta ataaacgacg tgatcattta ccaatcactt
    13081 ctttatttaa tcaataatac aacttaataa gccaacaatg atccaattag agaatatctt
    13141 taattagatg ttctcttaac tgtaagcgaa ctttattttc aatgaaatgt aattaattaa
    13201 gtaagtggga ctaaaacttt tcttttttag tcccattttt ataaacaatt attattcatt
    13261 gttactacta tcagcaattt tttcttttta gtgaaaccca agtatttgta agcatgataa
    13321 attacttctt tttatattta ttgggtttat ttgtattaat gtagttggaa tacatcttta
    13381 acaattcttc tgtttcatca ggacttaaat gctcacattc aaaatacttt tcaagcattg
    13441 caccttttcg aatcagtcta tgtgctcttt cttttctatc taaatttttt aatctatgct
    13501 ttcttaattc tgctttttca atttcagcat ttattttttt gagatgttta aaatcattca
    13561 tcgtatcact
//
