LOCUS       NZ_CP073839            13570 bp    DNA     circular CON 17-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1264454 plasmid p1B1264454,
            complete sequence.
ACCESSION   NZ_CP073839
VERSION     NZ_CP073839.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699877
            Assembly: GCF_019329525.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 273x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019329525.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 06:49:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,671
            CDSs (total)                      :: 2,590
            Genes (coding)                    :: 2,510
            CDSs (with protein)               :: 2,510
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 43 of 80
            Pseudo Genes (incomplete)         :: 43 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 24 of 80
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073839.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1264454"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p1B1264454"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(13483..13570,1..182))
                     /locus_tag="KFV39_RS00005"
                     /old_locus_tag="KFV39_00005"
     CDS             complement(join(13483..13570,1..182))
                     /locus_tag="KFV39_RS00005"
                     /old_locus_tag="KFV39_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            391..2313
                     /locus_tag="KFV39_RS00010"
                     /old_locus_tag="KFV39_00010"
     CDS             391..2313
                     /locus_tag="KFV39_RS00010"
                     /old_locus_tag="KFV39_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            2587..3000
                     /locus_tag="KFV39_RS13415"
                     /old_locus_tag="KFV39_00015"
     CDS             2587..3000
                     /locus_tag="KFV39_RS13415"
                     /old_locus_tag="KFV39_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            3077..3310
                     /locus_tag="KFV39_RS00020"
                     /old_locus_tag="KFV39_00020"
     CDS             3077..3310
                     /locus_tag="KFV39_RS00020"
                     /old_locus_tag="KFV39_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(3359..4063)
                     /locus_tag="KFV39_RS00025"
                     /old_locus_tag="KFV39_00025"
     CDS             complement(3359..4063)
                     /locus_tag="KFV39_RS00025"
                     /old_locus_tag="KFV39_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(4214..4912)
                     /locus_tag="KFV39_RS00030"
                     /old_locus_tag="KFV39_00030"
     CDS             complement(4214..4912)
                     /locus_tag="KFV39_RS00030"
                     /old_locus_tag="KFV39_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            5076..5174
                     /locus_tag="KFV39_RS00035"
                     /old_locus_tag="KFV39_00035"
     CDS             5076..5174
                     /locus_tag="KFV39_RS00035"
                     /old_locus_tag="KFV39_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(5351..6262)
                     /locus_tag="KFV39_RS00040"
                     /old_locus_tag="KFV39_00040"
     CDS             complement(5351..6262)
                     /locus_tag="KFV39_RS00040"
                     /old_locus_tag="KFV39_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(6681..7241)
                     /locus_tag="KFV39_RS00045"
                     /old_locus_tag="KFV39_00045"
     CDS             complement(6681..7241)
                     /locus_tag="KFV39_RS00045"
                     /old_locus_tag="KFV39_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            complement(7492..8058)
                     /gene="qacR"
                     /locus_tag="KFV39_RS00050"
                     /old_locus_tag="KFV39_00050"
     ARG             complement(7496..8058)
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            8237..9781
                     /gene="qac"
                     /locus_tag="KFV39_RS00055"
                     /old_locus_tag="KFV39_00055"
     CDS             8237..9781
                     /gene="qac"
                     /locus_tag="KFV39_RS00055"
                     /old_locus_tag="KFV39_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            9986..10546
                     /locus_tag="KFV39_RS00060"
                     /old_locus_tag="KFV39_00060"
     CDS             9986..10546
                     /locus_tag="KFV39_RS00060"
                     /old_locus_tag="KFV39_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(10865..11626)
                     /locus_tag="KFV39_RS00070"
                     /old_locus_tag="KFV39_00070"
     CDS             complement(10865..11626)
                     /locus_tag="KFV39_RS00070"
                     /old_locus_tag="KFV39_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            12067..13002
                     /locus_tag="KFV39_RS00075"
                     /old_locus_tag="KFV39_00075"
     CDS             12067..13002
                     /locus_tag="KFV39_RS00075"
                     /old_locus_tag="KFV39_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            complement(13227..13466)
                     /locus_tag="KFV39_RS00080"
                     /old_locus_tag="KFV39_00080"
     CDS             complement(13227..13466)
                     /locus_tag="KFV39_RS00080"
                     /old_locus_tag="KFV39_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     ARG             8238..9778
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          12068..13002
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          392..2244
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 tccataattt tatacaattc atcactagag ttgatcatat ttaaatcaag tttatttaaa
       61 aaataaccac caattttatt agatagttgt tcttcttgtt ttttcttttg ctctttcagt
      121 tgtgcaattt gtttttctaa ttgacttaat tttgtaatat ctgtttgttt aattttagac
      181 attaaaaaac tcccttagtt tatctatata aagcatacca taattcattg ggtaagtcgc
      241 acatccatgg tataatataa ccagttctta agacactgca ctgcccattt acatgcaaat
      301 tgaaaattgg cataaaaatg ggcaaccata ctggttgatc attatagttc ccacaggggt
      361 aaaaaaacag aaaagagagg aggtaaaatc atgaataaag gttactttat gttgagagtg
      421 actaacatta atcgagcaac aaaaaatatc gttgcgtcag cgtcttatcg ttctgatgaa
      481 gcattatata gcgaacgtac agatgaaaaa attaagttta gaaatcatac tattaagcct
      541 gaaagcatga ttttaactcc tcaacatgca cccgaatgga ctaaagatag acagcgttta
      601 tggaatgaag ttgataaagt tgaaaagaat aatgcgaaaa caaaaaatcc tagattagca
      661 aaagaagtct tattaagttt gcctaatgat tttgatagag aggtacaaac tgaattaact
      721 aaagatttta taaaaagtga gtttgtagac aaaggaatgg ttgctgatat ttcaattcat
      781 agagatgata taaacaaccc acacgcccat gttctactta ctcaaagacc gtttaatgca
      841 gacggtactt gggggaaaaa gacaaaaacg agaacgcaat atgatgaaaa tggcaatgcg
      901 attttaaata aaaatggtaa taaagttaga aaacaagaac gatttgccga ctttgatttc
      961 aaagaagttc gtaagcattg ggaattaaaa ttaaatcaat actcagaacg tgaacaaagt
     1021 ttaagacgat atgatagtcg ttcttttgaa gcgcaaggtt tagaaaaaat tgccgaaatt
     1081 ccacttacaa gagaagaata tagattagaa caaaatgaaa gaaaacgctg tgaaaaagag
     1141 ggtatcgagt accaacccgt tacttattac ggacagaaaa atgaacaaat tagaagatat
     1201 aatcgtggcg aagtatcaca attatttagt gataaagaga aagaacaagc acaaaatgaa
     1261 attagattat ttattcaaaa tgctaacgaa caagtaagta aaaatgaaca aagttattct
     1321 attttatttg atagatatca acgtgatgtt ggctattcag aggcgaaaga aacaattaaa
     1381 aatacgtttg acagtgcgtc tacctttggt cgtaaaattc aaaatgattt acttaaaaat
     1441 gaaattaaga gacgtcaatt aaaattctat gctaaaaatt atgatgaaaa ttatcagttt
     1501 agaaaagaaa ttgaaagtaa aggatatagt caacaagagt ttgttaatac tgttgaaact
     1561 aactataaag cgttactcaa tgagaagaat aaattagaaa gtagagagga aaaacgtaaa
     1621 gagatttatc aatcagctaa agatgtttat ctaactgaaa gtgaaaaaaa tcatctcatt
     1681 gttcagcaaa tgtaccctaa atctcatcat cattttagcc atgatgaaaa agcatttatt
     1741 gttgatgagg cacaaaaagg taatattata aaagcaactc atgttcaata cctgttcaaa
     1801 caaaataaca atattcctaa tgatgtattg ctagaagata cttataaaaa agcgagtaaa
     1861 gatattttct ttagtcaaaa acatcttaaa aatttagaaa aagatagctt tgaatataac
     1921 gttgagttgc aatttattaa ggaaaaagaa aaagaaattg atagttataa agaacatatt
     1981 gatgatgatt taagaataga tataggcgat aaaaactttg aaaaagtcaa agaccaaaca
     2041 cctaatgaaa aagttcaatt actcattaaa cttgagaaat ataatcatgt cgatcaagaa
     2101 gaagttatcg aaaaacatat taatcaagta gatagaaatg atcatgacaa tcgtattgaa
     2161 catgaaaatg aacaaactaa tcacattaat ttaccaacga taaatgctag tgactttctt
     2221 atgtactatg cgaacgaaac tgaaaagcaa aacaatatta atcatcagaa aaagaagaag
     2281 aaacaagaga gaaataacca aatgagaaga tagtaagcag ttgaaatcat acaaagttca
     2341 ttctttaata ataagaaact taacccaatg tggtaaatta gctttggttt aaagaaaaca
     2401 tttatatact tagataaggt acattctatg tattatcaca atgtatttta aaacgtgtat
     2461 aattatagaa aaaccttgat attatgcgat tgttcacata tttcagcata aaaaatacta
     2521 atgttcataa atatcgatag aaaaaattga gtttaaatat atcttaaagt taaggagaaa
     2581 aataatttgg aaacaaaaat tggacatcaa atacaaaagt taagaaaaag taaaaacatg
     2641 tctcaagaaa aattagctga aaagttaggg gtatcgagac attctatttc taattgggaa
     2701 agagaagtaa gtaatcccga cttaaaaaca attttagaaa tcactaaatt atttaatgtt
     2761 tctctaaatc aattaattaa aggagtggaa atcatgcaag ttaataaata tgtttactct
     2821 gcattagcat tttttctagg aggtttcggg gctcataagt tttatgttaa gaaaaataaa
     2881 aatgctcttc tttatttact attttgttgg acgggaatac cgggtgtgat aggtatggta
     2941 gaaggtgttt taacacttat gcgtccgagc gatagtgaaa acaatataga aataaattaa
     3001 accaaaaata aaaagccgat gtatcggatt ttttttagtg tacatgtgaa tattaaaaat
     3061 gatggagtga tttcacatga acacattcgt tttcggcgtg attacaaagc gattagaaga
     3121 aataggtttt aatgtacaca aggtgtttaa aatgcccgaa aaacaggcac tatttgaaga
     3181 tttgtaccat tatgtgagat acgttaaacc atgcaaggct gaactaacaa tggtggcatt
     3241 agatagtgat gaattgattc attactttat atgctgttac atggaacgtt atgcttatct
     3301 tttgaactaa tgataaaaaa gacgcctaat tttaggcgcc tttttaataa tctactaact
     3361 acttattata tctaatttta cttgctaact tagctactgt tttattgtcg tagggtttaa
     3421 tatagactgt ttcaatattc tcctctattg ctttttttgt tatcttattc atttgactat
     3481 aatcactatt ttgtgaattt tcccatttat aagggatatt attatataca ttaattgttc
     3541 catcaccatt actgctatat gttacagacc caccagctct cataggagct tctaatttca
     3601 caactttttc tttataaatt gcactatttt tggcttgtgg atttactttt gagccttttg
     3661 gtatttctgt aacataaaca tttttaatac cttttaaatc atttcttgta gatattaact
     3721 gataccaaac tcgtgcatat tcaatttctt tcgagttaaa attatttaag taacttttat
     3781 ttttatcagt agattgttgc ttcttattac tatcattatt tttttcttgt tttttattat
     3841 catttttttc tttattttgt ttattactac tttctgaatt atccgcacta tcagatgtgt
     3901 tagttttttc gtcaatttca tttgatgctt cattatcttt gttttctgag gtgttagtat
     3961 cactagaatt attatcattg tgatgttctg aatcgttatt attacaactc actaaaatta
     4021 ccgataaact aacaattaat ataggtaaaa tgattttttt catattacaa ctccttttca
     4081 gtatttcaaa ttagattatg ttaatatttt ttcagcaaga aaacgtaaat tacatgagtt
     4141 gaaaaatatg tattaaatat ataataaaat aaccacatgt tttacataaa cacggtggtt
     4201 attttatcaa tttttatatt gccttttttc tgaatattaa aagacttata cctaatatta
     4261 gtagtccaga agcgccaatt attgacaata tatcactttg gttgctttct cctgtttctg
     4321 gtaatacttg agagtttgaa tcttgatttt tcattccttc agaactcttg tttttatttt
     4381 cagttacagg tgtcaacaca ccttgaggtg tattaatagt tttattttct tcattagcta
     4441 tcttttgact ttcatttttt acaatttgat tatatttttc tgtggataca ctattagggt
     4501 taggaacatg actttctaat tcttggtatt cttcttgagt catacaatta ggtatcaccc
     4561 ctccgaaagg tcttttacct tgttgagttc tctcattatt ttgtatatca gtactttgtg
     4621 attgatattc attaaataca cttgaaactt tctcattata ctcgatttga gttaattccc
     4681 ctgaatcttt ctgtttagaa agttcactta tttgattttg tgcagattgt gggagggatg
     4741 aagtattgaa gtctacatta gcccaaccat ctccatttgt atctgcgcta tttggtaagt
     4801 tctctccgta tacggttttg gcaccatcct cagcattggc tgtcgctgaa aaacctgtga
     4861 atgttaatga gctagccaat aataaagttg gaagtaatga tcgttttttc ataaaattat
     4921 ctcctttaat agaatttgta ttttcattat cacatagata cattaaacat aaaatgactt
     4981 atcggaagtg catatgctta acaattcaca aatgatataa tataaaagtc gtcgttctac
     5041 ttctgtgctg gtttagcatg gaaggaggtg acaccatgtt cagcatcata tttgtatcta
     5101 tagtagctcc tattatagta ggggttataa tcacgttgtt ttcacattgg ttgaataatc
     5161 gtgataaaca ataaagcgac gacacagtaa taacaccaaa aagagcccga taactactgg
     5221 aatagttatc gggctcggtg tttaccatgt tcaacacata tttgttatct aaattataac
     5281 atcatagtgt ggaggaatgc aaaattgaat aattagtatg ttaagtctat actatactga
     5341 ttagtaagta ctaattatca tctttgttat tgttacccct ttcattggct tttgaatcta
     5401 attctttttg aaattttttg tgtgattttg aagaagtgtt taaaaagaaa taggtcatat
     5461 tatccatacg atgtcctttt ataaattcat tgataaattg tttttttgtc atttttttgg
     5521 ttgtattacg ttgtttttgc aatctaatga tttcttcttc aatgtatact cttatcatca
     5581 aatctaaact agatgtctga ttataagtag tgaataattt tggaactaat aaagttttat
     5641 taccatcaaa aagaaatgga gtgaaatcat aagtttccca tataccgtta gtagagaaat
     5701 aataatatgt cacagtttct ttagaaagaa acgatttcat ttctggaaat gactgaatca
     5761 tggatttatt aaaatcaatt ataacattca tgattatatt acttgttaaa tctgataatt
     5821 tatcatcacc aaaatttctg attaatggtg ctttttgagg tattgttaat ccgtcatcat
     5881 ttaagataat ggaaagaata tcttcattca taaatagatt aatcaattca gatgctgtag
     5941 taccttttcc tgtattacct ttctgagaaa aacctaagcg taaatggtgt ggttctgaaa
     6001 aatgagagaa taattttcta aattcattac gcttttgggt atcagaaaaa tcactaagat
     6061 gtatatcgat aattttttca aaaaaaacat caatttgctc ttgtgctctt tgagccaaag
     6121 tcgcaacttt tccaacttct ctattagata ttactttaat aaggtttggg tctatatatt
     6181 gaagagtgtc ttcttcaata gaaatattta aataatcagt tgatttagca tcatgattga
     6241 atttggtaca tactagtgtc actctatatc atccttctgt aagttttttg tttacttcca
     6301 atatagaatg atgtattatt gtaattgtcg atttacaata agttgttatt ctaggtggaa
     6361 gtttattgct tttgatgtat agatagactt aaccttttca ttatagcata ctatagaaga
     6421 aaatgcaagc aaaagcatat aaacaacaaa tgttgtttat atgcttttgc ttttttgtta
     6481 attggagagg acaaggtgaa tattaacagt caaaatccag ttatgtcggt taatgaatcg
     6541 atgaaaattt tcaaagctca agtaatgctt agaaatttaa agctattaag cgagtggatt
     6601 gttaaagggg actgaagtta aagacacttt ttaagaagat ttttcaatta aagggtatat
     6661 taaaacttta atttagtact ttatattttt cttgactctt taataatcct atatacagta
     6721 gaacgactca ctcctgtttg ttcagcaatt tcttcaccag acagcttttg ttcatcatat
     6781 aagaatttga tctctctttt cttatgatct ggtagtgaag gtcttccacc ttttctccct
     6841 cttgctcttg cagcttccaa acctttttta gttcgttcac ttaataaatt agcttcaagt
     6901 tcagcgaatg cactcatcat ggtaaagaac atcttgccca tcgcatcttt cgttgacaca
     6961 ttcatatcta taatatgtaa atcaatgcta ttttcatcca accattgaga tagctcaatt
     7021 aattgttttg ttgtacgtcc aagacgatct aatttataga caactagaat atctccttca
     7081 cgtaaataat ctaaacactt atcaagttcc gttcttttag ttttacgacc actcgctttc
     7141 tcgctaaaga tacgttcaca accatattcc tcaagtgtat caatctgtcc atcaagactt
     7201 tgatcttgtg ttgacacacg tgcataacca attttagcca tttgaacacc acctttttta
     7261 atcgttttat gggtacttac taatgaaata tagttctcga tgtaaataaa cacgtctgtc
     7321 agcatctaaa aactcatgat tttcgatatt tatgatacat tgattataac acaagtttat
     7381 agacttaaat atccaagaaa aacctcttaa tattaagtta agaggttttg tgtcaaaaac
     7441 gaacgtaatt tagaaataat atttattggt atttcaagaa tatatatata attatttact
     7501 aagtccattt aaaaaaattt gagagaactt gttcataagt ttaattcttt cattaatatt
     7561 ttgttcatgt gtaaaggtaa caattccatt aacagcgttt gctgctattt tactaacagc
     7621 attaacatca ttaatacacc attcgccatt taaattaccc tccttaaaaa ttacatgata
     7681 agcatctata tatttgtttt ctaatttatt cattttttca ttaatgctat tagttttata
     7741 atattcagtg taaaattcaa ttattgcgtt ttgaagtgga taatagtatt cggttgttaa
     7801 agatagttca ttatataaat aaaatttttc tctattagtt ttacatttga tttgttcctt
     7861 tttccactgt tcttgccatt tagattcttc tatatttaaa atttctaaaa atagattttc
     7921 ttttgtttta aagtgataat aaagattccc tttactactt tctgataatt taacaatttc
     7981 tccagtagta gtggcattat atccattttt tataaataat tcctttgcga cacctagtat
     8041 tttatctttc aagttcatca cgcctttaat gagtattaag tactatcaat gatagcacaa
     8101 aaaattcaaa gaataattgt ataacacaat acagccattt aaatttcgca agattttttg
     8161 ttgtaatatg taaaaaaaat agattataat ccttatagac cgatcgcacg gtctataagg
     8221 attggaggga acttaaatga tttcattttt tacaaaaact actgatatga tgacatcaaa
     8281 aaaaagatgg actgcactag tagtattagc tgttagtttg tttgttgtta caatggatat
     8341 gacaatatta attatggctt taccggaatt agtaagagag ttagagcctt ctggtaccca
     8401 acagttatgg atagttgata tatactctct tgttttagct ggctttataa ttccattgag
     8461 tgcctttgct gataaatggg gaagaaaaaa agcattatta actggatttg ctttatttgg
     8521 cctcgtttca ttagctatat ttttcgcaga aagtgcagag ttcgtaatag ctattcgatt
     8581 tttacttggt attgcaggtg ctttaataat gccaactact ctttcaatga taagagtaat
     8641 ttttgaaaac cctaaagaaa gggccactgc attagctgta tggtcaatcg cttcatcgat
     8701 aggtgctgtt tttggaccaa ttatcggagg agctttactt gagcaatttt catggcactc
     8761 ggcattttta attaatgtac cgtttgcgat aatagcagtt gtagcaggtt tatttttatt
     8821 accagagtct aagttatcaa aagaaaagtc tcactcgtgg gatattcctt ctacaatttt
     8881 atcaattgca ggcatgattg gactggtatg gagtatcaaa gaattttcaa aagaaggact
     8941 agcagatatt attccatggg ttgtaatagt attagcaatt accatgatag tgatatttgt
     9001 taaacgtaat ttatcaagtt ctgatccaat gttagacgta agacttttta aaaagagatc
     9061 attttcagct ggtacaattg ctgcatttat gacaattttt gcaatggcat ctgttttgtt
     9121 attagcttca caatggttac aggttgtgga agaactttct ccttttaaag ctggcttata
     9181 cctattacct atggcaatag gagatatggt gtttgcacca attgcacccg gattagcggc
     9241 gcgatttgga ccgaaaatag tgttaccttc cggaattgga attgcagcca ttggcatgtt
     9301 tattatgtat ttctttggtc atccattatc atattctaca atggctttag cattaatttt
     9361 agttggagct ggtatggctt cactagcagt tgcatctgct ctaataatgt tagaaacacc
     9421 tacatcaaaa gcaggtaatg cagctgctgt tgaagagtct atgtatgacc ttggaaatgt
     9481 ttttggtgta gcagtacttg gtagcctatc ttctatgctt tatcgtgtat ttttagatat
     9541 ttcatctttt tcatcaaaag gtatagttgg agatttagct catgtagctg aagaatctgt
     9601 agtgggcgct gtcgaagtag ctaaagctac ggggataaaa cagcttgcaa acgaggctgt
     9661 aacatcattt aatgatgctt ttgtagcaac tgctttagta ggtgggatta tcatgattat
     9721 catttcaata gttgtctatt tgttaattcc caaatcactt gatataacta aacaaaaata
     9781 gtactaaaca tatattttta gatattgtag attagtttat ttttaaaatt agtaatagtt
     9841 aaaaagtaga accattaatt ttaaatggtt ctactttttt attattaagt aaaattataa
     9901 aagacaattt cttaaattaa taatatgata tacctatatc taataattta ttagtattaa
     9961 taaaccttga aaggatgatt aactgatgaa tagagcagat atttttaaaa tatttgatgt
    10021 agaacttaat ataactatat ttttaggagc actagtagca actttcatct attttataag
    10081 ttacatgaac aatgtaaatg aattaattac attatgctta acattagctt ttcctgcata
    10141 tttattaaaa attagcagat cgttcgaggt tattttatta gaggaaaaaa taaaaaatag
    10201 taaaatcaaa atctgtgaca aagatagaat tgaacgagag atactggtga aaaagaacct
    10261 aatagatgaa ctattgtttc ctaatagagt tattaaaaaa tatacactga caattttaat
    10321 ttacagtata ggtattatta ttcttttact actttgtaat atgttaagtt atggaattgg
    10381 tgaaaatgtc aattttatct ggagattact agcttgttat gtaacgagtg tcacagtgat
    10441 tatagttgcc ttgattataa tgataatgat atttgatctt ataattggat tactattaaa
    10501 ttttcaaata tttttaaaaa ataaaaagta tgatgatttt ttataattat tcgatttata
    10561 taatgaaaaa ttaaatgatt aaacatagaa acctcaaaac aaaccatatt taatagagtt
    10621 tatggcgcct tatacaaata aaggctatgc tgttaaagaa ttatgtagct atcttcagat
    10681 taatatggat gatgtttata ctattggcga tagaaaattg atttacctat gtttaatacc
    10741 ctagataatt cttctgataa tttagttttt gttagagttt aacataactg atgattatca
    10801 gaagtttgat tcacattaaa aaggcacaac aagttcatga acttgtgtgc ctttttaatt
    10861 gcaattagtt accaaataaa cgactccaaa atctcttttt aggtggttct acctccctat
    10921 tatcactgga atcgttcttc gttttttcaa caaacttatc ctcttttgtt aattcatctg
    10981 tactgtcatg cttattctct tcagttatat ttttatgatt tacttcggtt ggttgacatt
    11041 ctttttgttg ttggttgata ttttgagatt cttcagtaaa agttgcttct tgtggattaa
    11101 tattctgtct attattcatc gttgtatcaa aagaatattt aagctgtctt tcttcttcta
    11161 attggctttc tagtttttga atttttttat tgttttctaa agataatatt tgttgattct
    11221 ccaacagttt atttaatgta ttaacattgc tattttgttg atctaattgt ttagttagat
    11281 tctcaatata tttttcatca tttttagctc tagtttcaaa gatttctatt tgcttttcta
    11341 attcgtttac ttttgttttt aatgtctcag aactatttga attattatgt atatactttt
    11401 caacttttgc agtattttca ttaaataatt cattttttac tgattcttta ttatgagttg
    11461 ttttctcttt cttagtgatt ttttctataa tttttaaata atcattatct ttaatataat
    11521 taattccatt ttctttttca aaagatatat ttaatctttt agcattatta acaatagttt
    11581 gtttagttac acctaattca tcagcaatca ttttaatagt tttcataaca tcccccctta
    11641 ggtacctaat ttagtaccta aattttacca cctaacaaaa tttatttcat catttagtac
    11701 taaatttact atctattaac tacctaaatg cctaattatc tatccttaaa atattttcgc
    11761 cacttaatta ggtgctaaaa tagcgaatta tacgtttggt agttttaggt gtacttttaa
    11821 ttacatttaa aactctttat atacgccatt aaaagtgtta atattacttt ataaatatta
    11881 aaagagtcga tgctattggc gtagcatcga ctctcggtaa taaaacgatt cgcactcgtt
    11941 tgtttatata tttttttgat acttgtatta tatatatcta atcatctaag tgcaagcaca
    12001 aaacatataa cttacgtaaa aattgtttta ttacctcaat cccaaaatgg aaatgaggtt
    12061 tttattatgc ccaattttga aaaatataat ttatcacaag taaaaactga aagattttat
    12121 caactgccta aatatttatt tgaagatgca tattttaaga aaatgtctgc agaagccaaa
    12181 attatgtatg cgttattaaa agatcgtttt gaattatccc tccaaaatga atgggtagat
    12241 aaaaataata atatttactt tattttcagt aataaacatt tgtgtgaata cttaggttat
    12301 gcagaacaaa aaattataaa attaaaaaaa gagttaataa aatttaattt actaactcaa
    12361 gaacgtgttg gccttaataa accaaataga ttatacctat taaaacctaa ttatgacatt
    12421 gaagccagtc atatcaagga acttccaaat tcacagttcc agaacaatga atttggaagt
    12481 tctagaactg tgaatttaag tggtcaagaa cttccaaatt cacagtctaa tgatactgat
    12541 tataatgaca ctgattatat taagactaat tataatgata tgtatgattt gaatgatagg
    12601 aaattaacat attctagtaa tcatacaaat cattcaaatc actactattc aaaattcaat
    12661 gatgaagctt taaaatttca attacttgaa gaactaccac aaagtattca aagttattta
    12721 agtaactttt ctgtatctga aattaaactt attaaatctg tgttattaaa agccaaaaca
    12781 tccttcaaca attctattga tgcatattat ttattagaag atatggaatt tgaaattgtt
    12841 aatgttctta agcgttttaa agctacatta attcaaaaaa atgaaaccgt tgaagcaatg
    12901 caagggtact taatgaaatc tcttaaatct gaatttgcag aagtacatac gcttaataaa
    12961 cgacgtgatc atttaccaat cacttcttta tttaatcaat aatacaactt aataagccaa
    13021 caatgatcca attagagaat atctttaatt agatgttctc ttaactgtaa gcgaacttta
    13081 ttttcaatga aatgtaatta attaagtaag tgggactaaa acttttcttt tttagtccca
    13141 tttttataaa caattattat tcattgttac tactatcagc aattttttct ttttagtgaa
    13201 acccaagtat ttgtaagcat gataaattac ttctttttat atttattggg tttatttgta
    13261 ttaatgtagt tggaatacat ctttaacaat tcttctgttt catcaggact taaatgctca
    13321 cattcaaaat acttttcaag cattgcacct tttcgaatca gtctatgtgc tctttctttt
    13381 ctatctaaat tttttaatct atgctttctt aattctgctt tttcaatttc agcatttatt
    13441 tttttgagat gtttaaaatc attcatcgta tcacttacta ttctagttaa ttttgtcttc
    13501 tgttaaattt tccttgttct cgttgctgct attttgttcg atatctttat taaattctcc
    13561 aatgacttta
//
