LOCUS       NZ_CP073837            13570 bp    DNA     circular CON 16-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1262351 plasmid p1B1262351,
            complete sequence.
ACCESSION   NZ_CP073837
VERSION     NZ_CP073837.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699876
            Assembly: GCF_019329505.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 254x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019329505.1-RS_2024_04_16
            Annotation Date                   :: 04/16/2024 07:57:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,600
            Genes (coding)                    :: 2,521
            CDSs (with protein)               :: 2,521
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 41 of 79
            Pseudo Genes (incomplete)         :: 44 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 24 of 79
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073837.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1262351"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p1B1262351"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(13538..13570,1..237))
                     /locus_tag="KFV42_RS13185"
                     /old_locus_tag="KFV42_13195"
     CDS             complement(join(13538..13570,1..237))
                     /locus_tag="KFV42_RS13185"
                     /old_locus_tag="KFV42_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            446..2368
                     /locus_tag="KFV42_RS13190"
                     /old_locus_tag="KFV42_13200"
     CDS             446..2368
                     /locus_tag="KFV42_RS13190"
                     /old_locus_tag="KFV42_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            2642..3055
                     /locus_tag="KFV42_RS13535"
                     /old_locus_tag="KFV42_13205"
     CDS             2642..3055
                     /locus_tag="KFV42_RS13535"
                     /old_locus_tag="KFV42_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            3132..3365
                     /locus_tag="KFV42_RS13200"
                     /old_locus_tag="KFV42_13210"
     CDS             3132..3365
                     /locus_tag="KFV42_RS13200"
                     /old_locus_tag="KFV42_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(3414..4118)
                     /locus_tag="KFV42_RS13205"
                     /old_locus_tag="KFV42_13215"
     CDS             complement(3414..4118)
                     /locus_tag="KFV42_RS13205"
                     /old_locus_tag="KFV42_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(4269..4967)
                     /locus_tag="KFV42_RS13210"
                     /old_locus_tag="KFV42_13220"
     CDS             complement(4269..4967)
                     /locus_tag="KFV42_RS13210"
                     /old_locus_tag="KFV42_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            5131..5229
                     /locus_tag="KFV42_RS13215"
                     /old_locus_tag="KFV42_13225"
     CDS             5131..5229
                     /locus_tag="KFV42_RS13215"
                     /old_locus_tag="KFV42_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(5406..6317)
                     /locus_tag="KFV42_RS13220"
                     /old_locus_tag="KFV42_13230"
     CDS             complement(5406..6317)
                     /locus_tag="KFV42_RS13220"
                     /old_locus_tag="KFV42_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(6736..7296)
                     /locus_tag="KFV42_RS13225"
                     /old_locus_tag="KFV42_13235"
     CDS             complement(6736..7296)
                     /locus_tag="KFV42_RS13225"
                     /old_locus_tag="KFV42_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            complement(7547..8113)
                     /gene="qacR"
                     /locus_tag="KFV42_RS13230"
                     /old_locus_tag="KFV42_13240"
     ARG             complement(7551..8113)
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            8292..9836
                     /gene="qac"
                     /locus_tag="KFV42_RS13235"
                     /old_locus_tag="KFV42_13245"
     CDS             8292..9836
                     /gene="qac"
                     /locus_tag="KFV42_RS13235"
                     /old_locus_tag="KFV42_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            10041..10601
                     /locus_tag="KFV42_RS13240"
                     /old_locus_tag="KFV42_13250"
     CDS             10041..10601
                     /locus_tag="KFV42_RS13240"
                     /old_locus_tag="KFV42_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(10920..11681)
                     /locus_tag="KFV42_RS13250"
                     /old_locus_tag="KFV42_13260"
     CDS             complement(10920..11681)
                     /locus_tag="KFV42_RS13250"
                     /old_locus_tag="KFV42_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            12122..13057
                     /locus_tag="KFV42_RS13255"
                     /old_locus_tag="KFV42_13265"
     CDS             12122..13057
                     /locus_tag="KFV42_RS13255"
                     /old_locus_tag="KFV42_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            complement(13282..13521)
                     /locus_tag="KFV42_RS13260"
                     /old_locus_tag="KFV42_13270"
     CDS             complement(13282..13521)
                     /locus_tag="KFV42_RS13260"
                     /old_locus_tag="KFV42_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     ARG             8293..9833
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          12123..13057
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          447..2299
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 gttctcgttg ctgctatttt gttcgatatc tttattaaat tctccaatga ctttatccat
       61 aattttatac aattcatcac tagagttgat catatttaaa tcaagtttat ttaaaaaata
      121 accaccaatt ttattagata gttgttcttc ttgttttttc ttttgctctt tcagttgtgc
      181 aatttgtttt tctaattgac ttaattttgt aatatctgtt tgtttaattt tagacattaa
      241 aaaactccct tagtttatct atataaagca taccataatt cattgggtaa gtcgcacatc
      301 catggtataa tataaccagt tcttaagaca ctgcactgcc catttacatg caaattgaaa
      361 attggcataa aaatgggcaa ccatactggt tgatcattat agttcccaca ggggtaaaaa
      421 aacagaaaag agaggaggta aaatcatgaa taaaggttac tttatgttga gagtgactaa
      481 cattaatcga gcaacaaaaa atatcgttgc gtcagcgtct tatcgttctg atgaagcatt
      541 atatagcgaa cgtacagatg aaaaaattaa gtttagaaat catactatta agcctgaaag
      601 catgatttta actcctcaac atgcacccga atggactaaa gatagacagc gtttatggaa
      661 tgaagttgat aaagttgaaa agaataatgc gaaaacaaaa aatcctagat tagcaaaaga
      721 agtcttatta agtttgccta atgattttga tagagaggta caaactgaat taactaaaga
      781 ttttataaaa agtgagtttg tagacaaagg aatggttgct gatatttcaa ttcatagaga
      841 tgatataaac aacccacacg cccatgttct acttactcaa agaccgttta atgcagacgg
      901 tacttggggg aaaaagacaa aaacgagaac gcaatatgat gaaaatggca atgcgatttt
      961 aaataaaaat ggtaataaag ttagaaaaca agaacgattt gccgactttg atttcaaaga
     1021 agttcgtaag cattgggaat taaaattaaa tcaatactca gaacgtgaac aaagtttaag
     1081 acgatatgat agtcgttctt ttgaagcgca aggtttagaa aaaattgccg aaattccact
     1141 tacaagagaa gaatatagat tagaacaaaa tgaaagaaaa cgctgtgaaa aagagggtat
     1201 cgagtaccaa cccgttactt attacggaca gaaaaatgaa caaattagaa gatataatcg
     1261 tggcgaagta tcacaattat ttagtgataa agagaaagaa caagcacaaa atgaaattag
     1321 attatttatt caaaatgcta acgaacaagt aagtaaaaat gaacaaagtt attctatttt
     1381 atttgataga tatcaacgtg atgttggcta ttcagaggcg aaagaaacaa ttaaaaatac
     1441 gtttgacagt gcgtctacct ttggtcgtaa aattcaaaat gatttactta aaaatgaaat
     1501 taagagacgt caattaaaat tctatgctaa aaattatgat gaaaattatc agtttagaaa
     1561 agaaattgaa agtaaaggat atagtcaaca agagtttgtt aatactgttg aaactaacta
     1621 taaagcgtta ctcaatgaga agaataaatt agaaagtaga gaggaaaaac gtaaagagat
     1681 ttatcaatca gctaaagatg tttatctaac tgaaagtgaa aaaaatcatc tcattgttca
     1741 gcaaatgtac cctaaatctc atcatcattt tagccatgat gaaaaagcat ttattgttga
     1801 tgaggcacaa aaaggtaata ttataaaagc aactcatgtt caatacctgt tcaaacaaaa
     1861 taacaatatt cctaatgatg tattgctaga agatacttat aaaaaagcga gtaaagatat
     1921 tttctttagt caaaaacatc ttaaaaattt agaaaaagat agctttgaat ataacgttga
     1981 gttgcaattt attaaggaaa aagaaaaaga aattgatagt tataaagaac atattgatga
     2041 tgatttaaga atagatatag gcgataaaaa ctttgaaaaa gtcaaagacc aaacacctaa
     2101 tgaaaaagtt caattactca ttaaacttga gaaatataat catgtcgatc aagaagaagt
     2161 tatcgaaaaa catattaatc aagtagatag aaatgatcat gacaatcgta ttgaacatga
     2221 aaatgaacaa actaatcaca ttaatttacc aacgataaat gctagtgact ttcttatgta
     2281 ctatgcgaac gaaactgaaa agcaaaacaa tattaatcat cagaaaaaga agaagaaaca
     2341 agagagaaat aaccaaatga gaagatagta agcagttgaa atcatacaaa gttcattctt
     2401 taataataag aaacttaacc caatgtggta aattagcttt ggtttaaaga aaacatttat
     2461 atacttagat aaggtacatt ctatgtatta tcacaatgta ttttaaaacg tgtataatta
     2521 tagaaaaacc ttgatattat gcgattgttc acatatttca gcataaaaaa tactaatgtt
     2581 cataaatatc gatagaaaaa attgagttta aatatatctt aaagttaagg agaaaaataa
     2641 tttggaaaca aaaattggac atcaaataca aaagttaaga aaaagtaaaa acatgtctca
     2701 agaaaaatta gctgaaaagt taggggtatc gagacattct atttctaatt gggaaagaga
     2761 agtaagtaat cccgacttaa aaacaatttt agaaatcact aaattattta atgtttctct
     2821 aaatcaatta attaaaggag tggaaatcat gcaagttaat aaatatgttt actctgcatt
     2881 agcatttttt ctaggaggtt tcggggctca taagttttat gttaagaaaa ataaaaatgc
     2941 tcttctttat ttactatttt gttggacggg aataccgggt gtgataggta tggtagaagg
     3001 tgttttaaca cttatgcgtc cgagcgatag tgaaaacaat atagaaataa attaaaccaa
     3061 aaataaaaag ccgatgtatc ggattttttt tagtgtacat gtgaatatta aaaatgatgg
     3121 agtgatttca catgaacaca ttcgttttcg gcgtgattac aaagcgatta gaagaaatag
     3181 gttttaatgt acacaaggtg tttaaaatgc ccgaaaaaca ggcactattt gaagatttgt
     3241 accattatgt gagatacgtt aaaccatgca aggctgaact aacaatggtg gcattagata
     3301 gtgatgaatt gattcattac tttatatgct gttacatgga acgttatgct tatcttttga
     3361 actaatgata aaaaagacgc ctaattttag gcgccttttt aataatctac taactactta
     3421 ttatatctaa ttttacttgc taacttagct actgttttat tgtcgtaggg tttaatatag
     3481 actgtttcaa tattctcctc tattgctttt tttgttatct tattcatttg actataatca
     3541 ctattttgtg aattttccca tttataaggg atattattat atacattaat tgttccatca
     3601 ccattactgc tatatgttac agacccacca gctctcatag gagcttctaa tttcacaact
     3661 ttttctttat aaattgcact atttttggct tgtggattta cttttgagcc ttttggtatt
     3721 tctgtaacat aaacattttt aatacctttt aaatcatttc ttgtagatat taactgatac
     3781 caaactcgtg catattcaat ttctttcgag ttaaaattat ttaagtaact tttattttta
     3841 tcagtagatt gttgcttctt attactatca ttattttttt cttgtttttt attatcattt
     3901 ttttctttat tttgtttatt actactttct gaattatccg cactatcaga tgtgttagtt
     3961 ttttcgtcaa tttcatttga tgcttcatta tctttgtttt ctgaggtgtt agtatcacta
     4021 gaattattat cattgtgatg ttctgaatcg ttattattac aactcactaa aattaccgat
     4081 aaactaacaa ttaatatagg taaaatgatt tttttcatat tacaactcct tttcagtatt
     4141 tcaaattaga ttatgttaat attttttcag caagaaaacg taaattacat gagttgaaaa
     4201 atatgtatta aatatataat aaaataacca catgttttac ataaacacgg tggttatttt
     4261 atcaattttt atattgcctt ttttctgaat attaaaagac ttatacctaa tattagtagt
     4321 ccagaagcgc caattattga caatatatca ctttggttgc tttctcctgt ttctggtaat
     4381 acttgagagt ttgaatcttg atttttcatt ccttcagaac tcttgttttt attttcagtt
     4441 acaggtgtca acacaccttg aggtgtatta atagttttat tttcttcatt agctatcttt
     4501 tgactttcat tttttacaat ttgattatat ttttctgtgg atacactatt agggttagga
     4561 acatgacttt ctaattcttg gtattcttct tgagtcatac aattaggtat cacccctccg
     4621 aaaggtcttt taccttgttg agttctctca ttattttgta tatcagtact ttgtgattga
     4681 tattcattaa atacacttga aactttctca ttatactcga tttgagttaa ttcccctgaa
     4741 tctttctgtt tagaaagttc acttatttga ttttgtgcag attgtgggag ggatgaagta
     4801 ttgaagtcta cattagccca accatctcca tttgtatctg cgctatttgg taagttctct
     4861 ccgtatacgg ttttggcacc atcctcagca ttggctgtcg ctgaaaaacc tgtgaatgtt
     4921 aatgagctag ccaataataa agttggaagt aatgatcgtt ttttcataaa attatctcct
     4981 ttaatagaat ttgtattttc attatcacat agatacatta aacataaaat gacttatcgg
     5041 aagtgcatat gcttaacaat tcacaaatga tataatataa aagtcgtcgt tctacttctg
     5101 tgctggttta gcatggaagg aggtgacacc atgttcagca tcatatttgt atctatagta
     5161 gctcctatta tagtaggggt tataatcacg ttgttttcac attggttgaa taatcgtgat
     5221 aaacaataaa gcgacgacac agtaataaca ccaaaaagag cccgataact actggaatag
     5281 ttatcgggct cggtgtttac catgttcaac acatatttgt tatctaaatt ataacatcat
     5341 agtgtggagg aatgcaaaat tgaataatta gtatgttaag tctatactat actgattagt
     5401 aagtactaat tatcatcttt gttattgtta cccctttcat tggcttttga atctaattct
     5461 ttttgaaatt ttttgtgtga ttttgaagaa gtgtttaaaa agaaataggt catattatcc
     5521 atacgatgtc cttttataaa ttcattgata aattgttttt ttgtcatttt tttggttgta
     5581 ttacgttgtt tttgcaatct aatgatttct tcttcaatgt atactcttat catcaaatct
     5641 aaactagatg tctgattata agtagtgaat aattttggaa ctaataaagt tttattacca
     5701 tcaaaaagaa atggagtgaa atcataagtt tcccatatac cgttagtaga gaaataataa
     5761 tatgtcacag tttctttaga aagaaacgat ttcatttctg gaaatgactg aatcatggat
     5821 ttattaaaat caattataac attcatgatt atattacttg ttaaatctga taatttatca
     5881 tcaccaaaat ttctgattaa tggtgctttt tgaggtattg ttaatccgtc atcatttaag
     5941 ataatggaaa gaatatcttc attcataaat agattaatca attcagatgc tgtagtacct
     6001 tttcctgtat tacctttctg agaaaaacct aagcgtaaat ggtgtggttc tgaaaaatga
     6061 gagaataatt ttctaaattc attacgcttt tgggtatcag aaaaatcact aagatgtata
     6121 tcgataattt tttcaaaaaa aacatcaatt tgctcttgtg ctctttgagc caaagtcgca
     6181 acttttccaa cttctctatt agatattact ttaataaggt ttgggtctat atattgaaga
     6241 gtgtcttctt caatagaaat atttaaataa tcagttgatt tagcatcatg attgaatttg
     6301 gtacatacta gtgtcactct atatcatcct tctgtaagtt ttttgtttac ttccaatata
     6361 gaatgatgta ttattgtaat tgtcgattta caataagttg ttattctagg tggaagttta
     6421 ttgcttttga tgtatagata gacttaacct tttcattata gcatactata gaagaaaatg
     6481 caagcaaaag catataaaca acaaatgttg tttatatgct tttgcttttt tgttaattgg
     6541 agaggacaag gtgaatatta acagtcaaaa tccagttatg tcggttaatg aatcgatgaa
     6601 aattttcaaa gctcaagtaa tgcttagaaa tttaaagcta ttaagcgagt ggattgttaa
     6661 aggggactga agttaaagac actttttaag aagatttttc aattaaaggg tatattaaaa
     6721 ctttaattta gtactttata tttttcttga ctctttaata atcctatata cagtagaacg
     6781 actcactcct gtttgttcag caatttcttc accagacagc ttttgttcat catataagaa
     6841 tttgatctct cttttcttat gatctggtag tgaaggtctt ccaccttttc tccctcttgc
     6901 tcttgcagct tccaaacctt ttttagttcg ttcacttaat aaattagctt caagttcagc
     6961 gaatgcactc atcatggtaa agaacatctt gcccatcgca tctttcgttg acacattcat
     7021 atctataata tgtaaatcaa tgctattttc atccaaccat tgagatagct caattaattg
     7081 ttttgttgta cgtccaagac gatctaattt atagacaact agaatatctc cttcacgtaa
     7141 ataatctaaa cacttatcaa gttccgttct tttagtttta cgaccactcg ctttctcgct
     7201 aaagatacgt tcacaaccat attcctcaag tgtatcaatc tgtccatcaa gactttgatc
     7261 ttgtgttgac acacgtgcat aaccaatttt agccatttga acaccacctt ttttaatcgt
     7321 tttatgggta cttactaatg aaatatagtt ctcgatgtaa ataaacacgt ctgtcagcat
     7381 ctaaaaactc atgattttcg atatttatga tacattgatt ataacacaag tttatagact
     7441 taaatatcca agaaaaacct cttaatatta agttaagagg ttttgtgtca aaaacgaacg
     7501 taatttagaa ataatattta ttggtatttc aagaatatat atataattat ttactaagtc
     7561 catttaaaaa aatttgagag aacttgttca taagtttaat tctttcatta atattttgtt
     7621 catgtgtaaa ggtaacaatt ccattaacag cgtttgctgc tattttacta acagcattaa
     7681 catcattaat acaccattcg ccatttaaat taccctcctt aaaaattaca tgataagcat
     7741 ctatatattt gttttctaat ttattcattt tttcattaat gctattagtt ttataatatt
     7801 cagtgtaaaa ttcaattatt gcgttttgaa gtggataata gtattcggtt gttaaagata
     7861 gttcattata taaataaaat ttttctctat tagttttaca tttgatttgt tcctttttcc
     7921 actgttcttg ccatttagat tcttctatat ttaaaatttc taaaaataga ttttcttttg
     7981 ttttaaagtg ataataaaga ttccctttac tactttctga taatttaaca atttctccag
     8041 tagtagtggc attatatcca ttttttataa ataattcctt tgcgacacct agtattttat
     8101 ctttcaagtt catcacgcct ttaatgagta ttaagtacta tcaatgatag cacaaaaaat
     8161 tcaaagaata attgtataac acaatacagc catttaaatt tcgcaagatt ttttgttgta
     8221 atatgtaaaa aaaatagatt ataatcctta tagaccgatc gcacggtcta taaggattgg
     8281 agggaactta aatgatttca ttttttacaa aaactactga tatgatgaca tcaaaaaaaa
     8341 gatggactgc actagtagta ttagctgtta gtttgtttgt tgttacaatg gatatgacaa
     8401 tattaattat ggctttaccg gaattagtaa gagagttaga gccttctggt acccaacagt
     8461 tatggatagt tgatatatac tctcttgttt tagctggctt tataattcca ttgagtgcct
     8521 ttgctgataa atggggaaga aaaaaagcat tattaactgg atttgcttta tttggcctcg
     8581 tttcattagc tatatttttc gcagaaagtg cagagttcgt aatagctatt cgatttttac
     8641 ttggtattgc aggtgcttta ataatgccaa ctactctttc aatgataaga gtaatttttg
     8701 aaaaccctaa agaaagggcc actgcattag ctgtatggtc aatcgcttca tcgataggtg
     8761 ctgtttttgg accaattatc ggaggagctt tacttgagca attttcatgg cactcggcat
     8821 ttttaattaa tgtaccgttt gcgataatag cagttgtagc aggtttattt ttattaccag
     8881 agtctaagtt atcaaaagaa aagtctcact cgtgggatat tccttctaca attttatcaa
     8941 ttgcaggcat gattggactg gtatggagta tcaaagaatt ttcaaaagaa ggactagcag
     9001 atattattcc atgggttgta atagtattag caattaccat gatagtgata tttgttaaac
     9061 gtaatttatc aagttctgat ccaatgttag acgtaagact ttttaaaaag agatcatttt
     9121 cagctggtac aattgctgca tttatgacaa tttttgcaat ggcatctgtt ttgttattag
     9181 cttcacaatg gttacaggtt gtggaagaac tttctccttt taaagctggc ttatacctat
     9241 tacctatggc aataggagat atggtgtttg caccaattgc acccggatta gcggcgcgat
     9301 ttggaccgaa aatagtgtta ccttccggaa ttggaattgc agccattggc atgtttatta
     9361 tgtatttctt tggtcatcca ttatcatatt ctacaatggc tttagcatta attttagttg
     9421 gagctggtat ggcttcacta gcagttgcat ctgctctaat aatgttagaa acacctacat
     9481 caaaagcagg taatgcagct gctgttgaag agtctatgta tgaccttgga aatgtttttg
     9541 gtgtagcagt acttggtagc ctatcttcta tgctttatcg tgtattttta gatatttcat
     9601 ctttttcatc aaaaggtata gttggagatt tagctcatgt agctgaagaa tctgtagtgg
     9661 gcgctgtcga agtagctaaa gctacgggga taaaacagct tgcaaacgag gctgtaacat
     9721 catttaatga tgcttttgta gcaactgctt tagtaggtgg gattatcatg attatcattt
     9781 caatagttgt ctatttgtta attcccaaat cacttgatat aactaaacaa aaatagtact
     9841 aaacatatat ttttagatat tgtagattag tttattttta aaattagtaa tagttaaaaa
     9901 gtagaaccat taattttaaa tggttctact tttttattat taagtaaaat tataaaagac
     9961 aatttcttaa attaataata tgatatacct atatctaata atttattagt attaataaac
    10021 cttgaaagga tgattaactg atgaatagag cagatatttt taaaatattt gatgtagaac
    10081 ttaatataac tatattttta ggagcactag tagcaacttt catctatttt ataagttaca
    10141 tgaacaatgt aaatgaatta attacattat gcttaacatt agcttttcct gcatatttat
    10201 taaaaattag cagatcgttc gaggttattt tattagagga aaaaataaaa aatagtaaaa
    10261 tcaaaatctg tgacaaagat agaattgaac gagagatact ggtgaaaaag aacctaatag
    10321 atgaactatt gtttcctaat agagttatta aaaaatatac actgacaatt ttaatttaca
    10381 gtataggtat tattattctt ttactacttt gtaatatgtt aagttatgga attggtgaaa
    10441 atgtcaattt tatctggaga ttactagctt gttatgtaac gagtgtcaca gtgattatag
    10501 ttgccttgat tataatgata atgatatttg atcttataat tggattacta ttaaattttc
    10561 aaatattttt aaaaaataaa aagtatgatg attttttata attattcgat ttatataatg
    10621 aaaaattaaa tgattaaaca tagaaacctc aaaacaaacc atatttaata gagtttatgg
    10681 cgccttatac aaataaaggc tatgctgtta aagaattatg tagctatctt cagattaata
    10741 tggatgatgt ttatactatt ggcgatagaa aattgattta cctatgttta ataccctaga
    10801 taattcttct gataatttag tttttgttag agtttaacat aactgatgat tatcagaagt
    10861 ttgattcaca ttaaaaaggc acaacaagtt catgaacttg tgtgcctttt taattgcaat
    10921 tagttaccaa ataaacgact ccaaaatctc tttttaggtg gttctacctc cctattatca
    10981 ctggaatcgt tcttcgtttt ttcaacaaac ttatcctctt ttgttaattc atctgtactg
    11041 tcatgcttat tctcttcagt tatattttta tgatttactt cggttggttg acattctttt
    11101 tgttgttggt tgatattttg agattcttca gtaaaagttg cttcttgtgg attaatattc
    11161 tgtctattat tcatcgttgt atcaaaagaa tatttaagct gtctttcttc ttctaattgg
    11221 ctttctagtt tttgaatttt tttattgttt tctaaagata atatttgttg attctccaac
    11281 agtttattta atgtattaac attgctattt tgttgatcta attgtttagt tagattctca
    11341 atatattttt catcattttt agctctagtt tcaaagattt ctatttgctt ttctaattcg
    11401 tttacttttg tttttaatgt ctcagaacta tttgaattat tatgtatata cttttcaact
    11461 tttgcagtat tttcattaaa taattcattt tttactgatt ctttattatg agttgttttc
    11521 tctttcttag tgattttttc tataattttt aaataatcat tatctttaat ataattaatt
    11581 ccattttctt tttcaaaaga tatatttaat cttttagcat tattaacaat agtttgttta
    11641 gttacaccta attcatcagc aatcatttta atagttttca taacatcccc ccttaggtac
    11701 ctaatttagt acctaaattt taccacctaa caaaatttat ttcatcattt agtactaaat
    11761 ttactatcta ttaactacct aaatgcctaa ttatctatcc ttaaaatatt ttcgccactt
    11821 aattaggtgc taaaatagcg aattatacgt ttggtagttt taggtgtact tttaattaca
    11881 tttaaaactc tttatatacg ccattaaaag tgttaatatt actttataaa tattaaaaga
    11941 gtcgatgcta ttggcgtagc atcgactctc ggtaataaaa cgattcgcac tcgtttgttt
    12001 atatattttt ttgatacttg tattatatat atctaatcat ctaagtgcaa gcacaaaaca
    12061 tataacttac gtaaaaattg ttttattacc tcaatcccaa aatggaaatg aggtttttat
    12121 tatgcccaat tttgaaaaat ataatttatc acaagtaaaa actgaaagat tttatcaact
    12181 gcctaaatat ttatttgaag atgcatattt taagaaaatg tctgcagaag ccaaaattat
    12241 gtatgcgtta ttaaaagatc gttttgaatt atccctccaa aatgaatggg tagataaaaa
    12301 taataatatt tactttattt tcagtaataa acatttgtgt gaatacttag gttatgcaga
    12361 acaaaaaatt ataaaattaa aaaaagagtt aataaaattt aatttactaa ctcaagaacg
    12421 tgttggcctt aataaaccaa atagattata cctattaaaa cctaattatg acattgaagc
    12481 cagtcatatc aaggaacttc caaattcaca gttccagaac aatgaatttg gaagttctag
    12541 aactgtgaat ttaagtggtc aagaacttcc aaattcacag tctaatgata ctgattataa
    12601 tgacactgat tatattaaga ctaattataa tgatatgtat gatttgaatg ataggaaatt
    12661 aacatattct agtaatcata caaatcattc aaatcactac tattcaaaat tcaatgatga
    12721 agctttaaaa tttcaattac ttgaagaact accacaaagt attcaaagtt atttaagtaa
    12781 cttttctgta tctgaaatta aacttattaa atctgtgtta ttaaaagcca aaacatcctt
    12841 caacaattct attgatgcat attatttatt agaagatatg gaatttgaaa ttgttaatgt
    12901 tcttaagcgt tttaaagcta cattaattca aaaaaatgaa accgttgaag caatgcaagg
    12961 gtacttaatg aaatctctta aatctgaatt tgcagaagta catacgctta ataaacgacg
    13021 tgatcattta ccaatcactt ctttatttaa tcaataatac aacttaataa gccaacaatg
    13081 atccaattag agaatatctt taattagatg ttctcttaac tgtaagcgaa ctttattttc
    13141 aatgaaatgt aattaattaa gtaagtggga ctaaaacttt tcttttttag tcccattttt
    13201 ataaacaatt attattcatt gttactacta tcagcaattt tttcttttta gtgaaaccca
    13261 agtatttgta agcatgataa attacttctt tttatattta ttgggtttat ttgtattaat
    13321 gtagttggaa tacatcttta acaattcttc tgtttcatca ggacttaaat gctcacattc
    13381 aaaatacttt tcaagcattg caccttttcg aatcagtcta tgtgctcttt cttttctatc
    13441 taaatttttt aatctatgct ttcttaattc tgctttttca atttcagcat ttattttttt
    13501 gagatgttta aaatcattca tcgtatcact tactattcta gttaattttg tcttctgtta
    13561 aattttcctt
//
