LOCUS       NZ_CP073833            13568 bp    DNA     circular CON 17-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1230143 plasmid p1B1230143,
            complete sequence.
ACCESSION   NZ_CP073833
VERSION     NZ_CP073833.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699875
            Assembly: GCF_019329425.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13568)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13568)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 368x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019329425.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 07:03:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,756
            CDSs (total)                      :: 2,675
            Genes (coding)                    :: 2,598
            CDSs (with protein)               :: 2,598
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 39 of 77
            Pseudo Genes (incomplete)         :: 45 of 77
            Pseudo Genes (internal stop)      :: 26 of 77
            Pseudo Genes (multiple problems)  :: 24 of 77
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073833.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13568
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1230143"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p1B1230143"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            63..1985
                     /locus_tag="KFV52_RS00005"
                     /old_locus_tag="KFV52_00005"
     CDS             63..1985
                     /locus_tag="KFV52_RS00005"
                     /old_locus_tag="KFV52_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            2259..2672
                     /locus_tag="KFV52_RS13870"
                     /old_locus_tag="KFV52_00010"
     CDS             2259..2672
                     /locus_tag="KFV52_RS13870"
                     /old_locus_tag="KFV52_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            2749..2982
                     /locus_tag="KFV52_RS00015"
                     /old_locus_tag="KFV52_00015"
     CDS             2749..2982
                     /locus_tag="KFV52_RS00015"
                     /old_locus_tag="KFV52_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(3031..3735)
                     /locus_tag="KFV52_RS00020"
                     /old_locus_tag="KFV52_00020"
     CDS             complement(3031..3735)
                     /locus_tag="KFV52_RS00020"
                     /old_locus_tag="KFV52_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(3886..4584)
                     /locus_tag="KFV52_RS00025"
                     /old_locus_tag="KFV52_00025"
     CDS             complement(3886..4584)
                     /locus_tag="KFV52_RS00025"
                     /old_locus_tag="KFV52_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            4748..4846
                     /locus_tag="KFV52_RS00030"
                     /old_locus_tag="KFV52_00030"
     CDS             4748..4846
                     /locus_tag="KFV52_RS00030"
                     /old_locus_tag="KFV52_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(5023..5934)
                     /locus_tag="KFV52_RS00035"
                     /old_locus_tag="KFV52_00035"
     CDS             complement(5023..5934)
                     /locus_tag="KFV52_RS00035"
                     /old_locus_tag="KFV52_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(6353..6913)
                     /locus_tag="KFV52_RS00040"
                     /old_locus_tag="KFV52_00040"
     CDS             complement(6353..6913)
                     /locus_tag="KFV52_RS00040"
                     /old_locus_tag="KFV52_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            complement(7164..7730)
                     /gene="qacR"
                     /locus_tag="KFV52_RS00045"
                     /old_locus_tag="KFV52_00045"
     ARG             complement(7168..7730)
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            7908..9452
                     /locus_tag="KFV52_RS00050"
                     /old_locus_tag="KFV52_00050"
     CDS             7908..9452
                     /locus_tag="KFV52_RS00050"
                     /old_locus_tag="KFV52_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /protein_id="WP_000622776.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTMFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="quaternary ammonium compound efflux MFS transporter
                     QacA"
     gene            9657..10217
                     /locus_tag="KFV52_RS00055"
                     /old_locus_tag="KFV52_00055"
     CDS             9657..10217
                     /locus_tag="KFV52_RS00055"
                     /old_locus_tag="KFV52_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(10536..11297)
                     /locus_tag="KFV52_RS00065"
                     /old_locus_tag="KFV52_00065"
     CDS             complement(10536..11297)
                     /locus_tag="KFV52_RS00065"
                     /old_locus_tag="KFV52_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            11737..12672
                     /locus_tag="KFV52_RS00070"
                     /old_locus_tag="KFV52_00070"
     CDS             11737..12672
                     /locus_tag="KFV52_RS00070"
                     /old_locus_tag="KFV52_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            complement(12897..13136)
                     /locus_tag="KFV52_RS00075"
                     /old_locus_tag="KFV52_00075"
     CDS             complement(12897..13136)
                     /locus_tag="KFV52_RS00075"
                     /old_locus_tag="KFV52_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(13153..13422)
                     /locus_tag="KFV52_RS00080"
                     /old_locus_tag="KFV52_00080"
     CDS             complement(13153..13422)
                     /locus_tag="KFV52_RS00080"
                     /old_locus_tag="KFV52_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     ARG             7909..9449
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          11738..12672
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          64..1916
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 tactggttga tcattatagt tcccacaggg gtaaaaaaac agaaaagaga ggaggtaaaa
       61 tcatgaataa aggttacttt atgttgagag tgactaacat taatcgagca acaaaaaata
      121 tcgttgcgtc agcgtcttat cgttctgatg aagcattata tagcgaacgt acagatgaaa
      181 aaattaagtt tagaaatcat actattaagc ctgaaagcat gattttaact cctcaacatg
      241 cacccgaatg gactaaagat agacagcgtt tatggaatga agttgataaa gttgaaaaga
      301 ataatgcgaa aacaaaaaat cctagattag caaaagaagt cttattaagt ttgcctaatg
      361 attttgatag agaggtacaa actgaattaa ctaaagattt tataaaaagt gagtttgtag
      421 acaaaggaat ggttgctgat atttcaattc atagagatga tataaacaac ccacacgccc
      481 atgttctact tactcaaaga ccgtttaatg cagacggtac ttgggggaaa aagacaaaaa
      541 cgagaacgca atatgatgaa aatggcaatg cgattttaaa taaaaatggt aataaagtta
      601 gaaaacaaga acgatttgcc gactttgatt tcaaagaagt tcgtaagcat tgggaattaa
      661 aattaaatca atactcagaa cgtgaacaaa gtttaagacg atatgatagt cgttcttttg
      721 aagcgcaagg tttagaaaaa attgccgaaa ttccacttac aagagaagaa tatagattag
      781 aacaaaatga aagaaaacgc tgtgaaaaag agggtatcga gtaccaaccc gttacttatt
      841 acggacagaa aaatgaacaa attagaagat ataatcgtgg cgaagtatca caattattta
      901 gtgataaaga gaaagaacaa gcacaaaatg aaattagatt atttattcaa aatgctaacg
      961 aacaagtaag taaaaatgaa caaagttatt ctattttatt tgatagatat caacgtgatg
     1021 ttggctattc agaggcgaaa gaaacaatta aaaatacgtt tgacagtgcg tctacctttg
     1081 gtcgtaaaat tcaaaatgat ttacttaaaa atgaaattaa gagacgtcaa ttaaaattct
     1141 atgctaaaaa ttatgatgaa aattatcagt ttagaaaaga aattgaaagt aaaggatata
     1201 gtcaacaaga gtttgttaat actgttgaaa ctaactataa agcgttactc aatgagaaga
     1261 ataaattaga aagtagagag gaaaaacgta aagagattta tcaatcagct aaagatgttt
     1321 atctaactga aagtgaaaaa aatcatctca ttgttcagca aatgtaccct aaatctcatc
     1381 atcattttag ccatgatgaa aaagcattta ttgttgatga ggcacaaaaa ggtaatatta
     1441 taaaagcaac tcatgttcaa tacctgttca aacaaaataa caatattcct aatgatgtat
     1501 tgctagaaga tacttataaa aaagcgagta aagatatttt ctttagtcaa aaacatctta
     1561 aaaatttaga aaaagatagc tttgaatata acgttgagtt gcaatttatt aaggaaaaag
     1621 aaaaagaaat tgatagttat aaagaacata ttgatgatga tttaagaata gatataggcg
     1681 ataaaaactt tgaaaaagtc aaagaccaaa cacctaatga aaaagttcaa ttactcatta
     1741 aacttgagaa atataatcat gtcgatcaag aagaagttat cgaaaaacat attaatcaag
     1801 tagatagaaa tgatcatgac aatcgtattg aacatgaaaa tgaacaaact aatcacatta
     1861 atttaccaac gataaatgct agtgactttc ttatgtacta tgcgaacgaa actgaaaagc
     1921 aaaacaatat taatcatcag aaaaagaaga agaaacaaga gagaaataac caaatgagaa
     1981 gatagtaagc agttgaaatc atacaaagtt cattctttaa taataagaaa cttaacccaa
     2041 tgtggtaaat tagctttggt ttaaagaaaa catttatata cttagataag gtacattcta
     2101 tgtattatca caatgtattt taaaacgtgt ataattatag aaaaaccttg atattatgcg
     2161 attgttcaca tatttcagca taaaaaatac taatgttcat aaatatcgat agaaaaaatt
     2221 gagtttaaat atatcttaaa gttaaggaga aaaataattt ggaaacaaaa attggacatc
     2281 aaatacaaaa gttaagaaaa agtaaaaaca tgtctcaaga aaaattagct gaaaagttag
     2341 gggtatcgag acattctatt tctaattggg aaagagaagt aagtaatccc gacttaaaaa
     2401 caattttaga aatcactaaa ttatttaatg tttctctaaa tcaattaatt aaaggagtgg
     2461 aaatcatgca agttaataaa tatgtttact ctgcattagc attttttcta ggaggtttcg
     2521 gggctcataa gttttatgtt aagaaaaata aaaatgctct tctttattta ctattttgtt
     2581 ggacgggaat accgggtgtg ataggtatgg tagaaggtgt tttaacactt atgcgtccga
     2641 gcgatagtga aaacaatata gaaataaatt aaaccaaaaa taaaaagccg atgtatcgga
     2701 ttttttttag tgtacatgtg aatattaaaa atgatggagt gatttcacat gaacacattc
     2761 gttttcggcg tgattacaaa gcgattagaa gaaataggtt ttaatgtaca caaggtgttt
     2821 aaaatgcccg aaaaacaggc actatttgaa gatttgtacc attatgtgag atacgttaaa
     2881 ccatgcaagg ctgaactaac aatggtggca ttagatagtg atgaattgat tcattacttt
     2941 atatgctgtt acatggaacg ttatgcttat cttttgaact aatgataaaa aagacgccta
     3001 attttaggcg cctttttaat aatctactaa ctacttatta tatctaattt tacttgctaa
     3061 cttagctact gttttattgt cgtagggttt aatatagact gtttcaatat tctcctctat
     3121 tgcttttttt gttatcttat tcatttgact ataatcacta ttttgtgaat tttcccattt
     3181 ataagggata ttattatata cattaattgt tccatcacca ttactgctat atgttacaga
     3241 cccaccagct ctcataggag cttctaattt cacaactttt tctttataaa ttgcactatt
     3301 tttggcttgt ggatttactt ttgagccttt tggtatttct gtaacataaa catttttaat
     3361 accttttaaa tcatttcttg tagatattaa ctgataccaa actcgtgcat attcaatttc
     3421 tttcgagtta aaattattta agtaactttt atttttatca gtagattgtt gcttcttatt
     3481 actatcatta tttttttctt gttttttatt atcatttttt tctttatttt gtttattact
     3541 actttctgaa ttatccgcac tatcagatgt gttagttttt tcgtcaattt catttgatgc
     3601 ttcattatct ttgttttctg aggtgttagt atcactagaa ttattatcat tgtgatgttc
     3661 tgaatcgtta ttattacaac tcactaaaat taccgataaa ctaacaatta atataggtaa
     3721 aatgattttt ttcatattac aactcctttt cagtatttca aattagatta tgttaatatt
     3781 ttttcagcaa gaaaacgtaa attacatgag ttgaaaaata tgtattaaat atataataaa
     3841 ataaccacat gttttacata aacacggtgg ttattttatc aatttttata ttgccttttt
     3901 tctgaatatt aaaagactta tacctaatat tagtagtcca gaagcgccaa ttattgacaa
     3961 tatatcactt tggttgcttt ctcctgtttc tggtaatact tgagagtttg aatcttgatt
     4021 tttcattcct tcagaactct tgtttttatt ttcagttaca ggtgtcaaca caccttgagg
     4081 tgtattaata gttttatttt cttcattagc tatcttttga ctttcatttt ttacaatttg
     4141 attatatttt tctgtggata cactattagg gttaggaaca tgactttcta attcttggta
     4201 ttcttcttga gtcatacaat taggtatcac ccctccgaaa ggtcttttac cttgttgagt
     4261 tctctcatta ttttgtatat cagtactttg tgattgatat tcattaaata cacttgaaac
     4321 tttctcatta tactcgattt gagttaattc ccctgaatct ttctgtttag aaagttcact
     4381 tatttgattt tgtgcagatt gtgggaggga tgaagtattg aagtctacat tagcccaacc
     4441 atctccattt gtatctgcgc tatttggtaa gttctctccg tatacggttt tggcaccatc
     4501 ctcagcattg gctgtcgctg aaaaacctgt gaatgttaat gagctagcca ataataaagt
     4561 tggaagtaat gatcgttttt tcataaaatt atctccttta atagaatttg tattttcatt
     4621 atcacataga tacattaaac ataaaatgac ttatcggaag tgcatatgct taacaattca
     4681 caaatgatat aatataaaag tcgtcgttct acttctgtgc tggtttagca tggaaggagg
     4741 tgacaccatg ttcagcatca tatttgtatc tatagtagct cctattatag taggggttat
     4801 aatcacgttg ttttcacatt ggttgaataa tcgtgataaa caataaagcg acgacacagt
     4861 aataacacca aaaagagccc gataactact ggaatagtta tcgggctcgg tgtttaccat
     4921 gttcaacaca tatttgttat ctaaattata acatcatagt gtggaggaat gcaaaattga
     4981 ataattagta tgttaagtct atactatact gattagtaag tactaattat catctttgtt
     5041 attgttaccc ctttcattgg cttttgaatc taattctttt tgaaattttt tgtgtgattt
     5101 tgaagaagtg tttaaaaaga aataggtcat attatccata cgatgtcctt ttataaattc
     5161 attgataaat tgtttttttg tcattttttt ggttgtatta cgttgttttt gcaatctaat
     5221 gatttcttct tcaatgtata ctcttatcat caaatctaaa ctagatgtct gattataagt
     5281 agtgaataat tttggaacta ataaagtttt attaccatca aaaagaaatg gagtgaaatc
     5341 ataagtttcc catataccgt tagtagagaa ataataatat gtcacagttt ctttagaaag
     5401 aaacgatttc atttctggaa atgactgaat catggattta ttaaaatcaa ttataacatt
     5461 catgattata ttacttgtta aatctgataa tttatcatca ccaaaatttc tgattaatgg
     5521 tgctttttga ggtattgtta atccgtcatc atttaagata atggaaagaa tatcttcatt
     5581 cataaataga ttaatcaatt cagatgctgt agtacctttt cctgtattac ctttctgaga
     5641 aaaacctaag cgtaaatggt gtggttctga aaaatgagag aataattttc taaattcatt
     5701 acgcttttgg gtatcagaaa aatcactaag atgtatatcg ataatttttt caaaaaaaac
     5761 atcaatttgc tcttgtgctc tttgagccaa agtcgcaact tttccaactt ctctattaga
     5821 tattacttta ataaggtttg ggtctatata ttgaagagtg tcttcttcaa tagaaatatt
     5881 taaataatca gttgatttag catcatgatt gaatttggta catactagtg tcactctata
     5941 tcatccttct gtaagttttt tgtttacttc caatatagaa tgatgtatta ttgtaattgt
     6001 cgatttacaa taagttgtta ttctaggtgg aagtttattg cttttgatgt atagatagac
     6061 ttaacctttt cattatagca tactatagaa gaaaatgcaa gcaaaagcat ataaacaaca
     6121 aatgttgttt atatgctttt gcttttttgt taattggaga ggacaaggtg aatattaaca
     6181 gtcaaaatcc agttatgtcg gttaatgaat cgatgaaaat tttcaaagct caagtaatgc
     6241 ttagaaattt aaagctatta agcgagtgga ttgttaaagg ggactgaagt taaagacact
     6301 ttttaagaag atttttcaat taaagggtat attaaaactt taatttagta ctttatattt
     6361 ttcttgactc tttaataatc ctatatacag tagaacgact cactcctgtt tgttcagcaa
     6421 tttcttcacc agacagcttt tgttcatcat ataagaattt gatctctctt ttcttatgat
     6481 ctggtagtga aggtcttcca ccttttctcc ctcttgctct tgcagcttcc aaaccttttt
     6541 tagttcgttc acttaataaa ttagcttcaa gttcagcgaa tgcactcatc atggtaaaga
     6601 acatcttgcc catcgcatct ttcgttgaca cattcatatc tataatatgt aaatcaatgc
     6661 tattttcatc caaccattga gatagctcaa ttaattgttt tgttgtacgt ccaagacgat
     6721 ctaatttata gacaactaga atatctcctt cacgtaaata atctaaacac ttatcaagtt
     6781 ccgttctttt agttttacga ccactcgctt tctcgctaaa gatacgttca caaccatatt
     6841 cctcaagtgt atcaatctgt ccatcaagac tttgatcttg tgttgacaca cgtgcataac
     6901 caattttagc catttgaaca ccaccttttt taatcgtttt atgggtactt actaatgaaa
     6961 tatagttctc gatgtaaata aacacgtctg tcagcatcta aaaactcatg attttcgata
     7021 tttatgatac attgattata acacaagttt atagacttaa atatccaaga aaaacctctt
     7081 aatattaagt taagaggttt tgtgtcaaaa acgaacgtaa tttagaaata atatttattg
     7141 gtatttcaag aatatatata taattattta ctaagtccat ttaaaaaaat ttgagagaac
     7201 ttgttcataa gtttaattct ttcattaata ttttgttcat gtgtaaaggt aacaattcca
     7261 ttaacagcgt ttgctgctat tttactaaca gcattaacat cattaataca ccattcgcca
     7321 tttaaattac cctccttaaa aattacatga taagcatcta tatatttgtt ttctaattta
     7381 ttcatttttt cattaatgct attagtttta taatattcag tgtaaaattc aattattgcg
     7441 ttttgaagtg gataatagta ttcggttgtt aaagatagtt cattatataa ataaaatttt
     7501 tctctattag ttttacattt gatttgttcc tttttccact gttcttgcca tttagattct
     7561 tctatattta aaatttctaa aaatagattt tcttttgttt taaagtgata ataaagattc
     7621 cctttactac tttctgataa tttaacaatt tctccagtag tagtggcatt atatccattt
     7681 tttataaata attcctttgc gacacctagt attttatctt tcaagttcat cacgccttta
     7741 atgagtatta agtactatca atgatagcac aaaaaattca aagaataatt gtataacaca
     7801 atacagccat ttaaatttcg caagattttt tgttgtaata tgtaaaaaaa tagattacaa
     7861 tccttataga ccgatcgcac ggtctataag gattggaggg aacttaaatg atttcatttt
     7921 ttacaaaaac tactgatatg atgacatcaa aaaaaagatg gactgcacta gtagtattag
     7981 ctgttagttt gtttgttgtt acaatggata tgacaatatt aattatggct ttaccggaat
     8041 tagtaagaga gttagagcct tctggtaccc aacagttatg gatagttgat atatactctc
     8101 ttgttttagc tggctttata attccattga gtgcctttgc tgataaatgg ggaagaaaaa
     8161 aagcattatt aactggattt gctttatttg gcctcgtttc attagctata tttttcgcag
     8221 aaagtgcaga gttcgtaata gctattcgat ttttacttgg tattgcaggt gctttaataa
     8281 tgccaactac tctttcaatg ataagagtaa tttttgaaaa ccctaaagaa agggccactg
     8341 cattagctgt atggtcaatc gcttcatcga taggtgctgt ttttggacca attatcggag
     8401 gagctttact tgagcaattt tcatggcact cggcattttt aattaatgta ccgtttgcga
     8461 taatagcagt tgtagcaggt ttatttttat taccagagtc taagttatca aaagaaaagt
     8521 ctcactcgtg ggatattcct tctacaattt tatcaattgc aggcatgatt ggactggtat
     8581 ggagtatcaa agaattttca aaagaaggac tagcagatat tattccatgg gttgtaatag
     8641 tattagcaat taccatgata gtgatatttg ttaaacgtaa tttatcaagt tctgatccaa
     8701 tgttagacgt aagacttttt aaaaagagat cattttcagc tggtacaatt gctgcattta
     8761 tgacaatgtt tgcaatggca tctgttttgt tattagcttc acaatggtta caggttgtgg
     8821 aagaactttc tccttttaaa gctggcttat acctattacc tatggcaata ggagatatgg
     8881 tgtttgcacc aattgcaccc ggattagcgg cgcgatttgg accgaaaata gtgttacctt
     8941 ccggaattgg aattgcagcc attggcatgt ttattatgta tttctttggt catccattat
     9001 catattctac aatggcttta gcattaattt tagttggagc tggtatggct tcactagcag
     9061 ttgcatctgc tctaataatg ttagaaacac ctacatcaaa agcaggtaat gcagctgctg
     9121 ttgaagagtc tatgtatgac cttggaaatg tttttggtgt agcagtactt ggtagcctat
     9181 cttctatgct ttatcgtgta tttttagata tttcatcttt ttcatcaaaa ggtatagttg
     9241 gagatttagc tcatgtagct gaagaatctg tagtgggcgc tgtcgaagta gctaaagcta
     9301 cggggataaa acagcttgca aacgaggctg taacatcatt taatgatgct tttgtagcaa
     9361 ctgctttagt aggtgggatt atcatgatta tcatttcaat agttgtctat ttgttaattc
     9421 ccaaatcact tgatataact aaacaaaaat agtactaaac atatattttt agatattgta
     9481 gattagttta tttttaaaat tagtaatagt taaaaagtag aaccattaat tttaaatggt
     9541 tctacttttt tattattaag taaaattata aaagacaatt tcttaaatta ataatatgat
     9601 atacctatat ctaataattt attagtatta ataaaccttg aaaggatgat taactgatga
     9661 atagagcaga tatttttaaa atatttgatg tagaacttaa tataactata tttttaggag
     9721 cactagtagc aactttcatc tattttataa gttacatgaa caatgtaaat gaattaatta
     9781 cattatgctt aacattagct tttcctgcat atttattaaa aattagcaga tcgttcgagg
     9841 ttattttatt agaggaaaaa ataaaaaata gtaaaatcaa aatctgtgac aaagatagaa
     9901 ttgaacgaga gatactggtg aaaaagaacc taatagatga actattgttt cctaatagag
     9961 ttattaaaaa atatacactg acaattttaa tttacagtat aggtattatt attcttttac
    10021 tactttgtaa tatgttaagt tatggaattg gtgaaaatgt caattttatc tggagattac
    10081 tagcttgtta tgtaacgagt gtcacagtga ttatagttgc cttgattata atgataatga
    10141 tatttgatct tataattgga ttactattaa attttcaaat atttttaaaa aataaaaagt
    10201 atgatgactt tttataatta ttcgatttat ataatgaaaa attaaatgat taaacataga
    10261 aacctcaaaa caaaccatat ttaatagagt ttatggcgcc ttatacaaat aaaggctatg
    10321 ctgttaaaga attatgtagc tatcttcaga ttaatatgga tgatgtttat actattggcg
    10381 atagaaaatt gatttaccta tgtttaatac cctagataat tcttctgata atttagtttt
    10441 tgttagagtt taacataact gatgattatc agaagtttga ttcacattaa aaaggcacaa
    10501 caagttcatg aacttgtgtg cctttttaat tgcaattagt taccaaataa acgactccaa
    10561 aatctctttt taggtggttc tacctcccta ttatcactgg aatcgttctt cgttttttca
    10621 acaaacttat cctcttttgt taattcatct gtactgtcat gcttattctc ttcagttata
    10681 tttttatgat ttacttcggt tggttgacat tctttttgtt gttggttgat attttgagat
    10741 tcttcagtaa aagttgcttc ttgtggatta atattctgtc tattattcat cgttgtatca
    10801 aaagaatatt taagctgtct ttcttcttct aattggcttt ctagtttttg aattttttta
    10861 ttgttttcta aagataatat ttgttgattc tccaacagtt tatttaatgt attaacattg
    10921 ctattttgtt gatctaattg tttagttaga ttctcaatat atttttcatc atttttagct
    10981 ctagtttcaa agatttctat ttgcttttct aattcgttta cttttgtttt taatgtctca
    11041 gaactatttg aattattatg tatatacttt tcaacttttg cagtattttc attaaataat
    11101 tcatttttta ctgattcttt attatgagtt gttttctctt tcttagtgat tttttctata
    11161 atttttaaat aatcattatc tttaatataa ttaattccat tttctttttc aaaagatata
    11221 tttaatcttt tagcattatt aacaatagtt tgtttagtta cacctaattc atcagcaatc
    11281 attttaatag ttttcataac atccccctta ggtacctaat ttagtaccta aattttacca
    11341 cctaacaaaa tttatttcat catttagtac taaatttact atctattaac tacctaaatg
    11401 cctaattatc tatccttaaa atattttcgc cacttaatta ggtgctaaaa tagcgaatta
    11461 tacgtttggt agttttaggt gtacttttaa ttacatttaa aactctttat atacgccatt
    11521 aaaagtgtta atattacttt ataaatatta aaagagtcga tgctattggc gtagcatcga
    11581 ctctcggtaa taaaacgatt cgcactcgtt tgtttatata tttttttgat acttgtatta
    11641 tatatatcta atcatctaag tgcaagcaca aaacatataa cttacgtaaa aattgtttta
    11701 ttacctcaat cccaaaatgg aaatgaggtt tttattatgc ccaattttga aaaatataat
    11761 ttatcacaag taaaaactga aagattttat caactgccta aatatttatt tgaagatgca
    11821 tattttaaga aaatgtctgc agaagccaaa attatgtatg cgttattaaa agatcgtttt
    11881 gaattatccc tccaaaatga atgggtagat aaaaataata atatttactt tattttcagt
    11941 aataaacatt tgtgtgaata cttaggttat gcagaacaaa aaattataaa attaaaaaaa
    12001 gagttaataa aatttaattt actaactcaa gaacgtgttg gccttaataa accaaataga
    12061 ttatacctat taaaacctaa ttatgacatt gaagccagtc atatcaagga acttccaaat
    12121 tcacagttcc agaacaatga atttggaagt tctagaactg tgaatttaag tggtcaagaa
    12181 cttccaaatt cacagtctaa tgatactgat tataatgaca ctgattatat taagactaat
    12241 tataatgata tgtatgattt gaatgatagg aaattaacat attctagtaa tcatacaaat
    12301 cattcaaatc actactattc aaaattcaat gatgaagctt taaaatttca attacttgaa
    12361 gaactaccac aaagtattca aagttattta agtaactttt ctgtatctga aattaaactt
    12421 attaaatctg tgttattaaa agccaaaaca tccttcaaca attctattga tgcatattat
    12481 ttattagaag atatggaatt tgaaattgtt aatgttctta agcgttttaa agctacatta
    12541 attcaaaaaa atgaaaccgt tgaagcaatg caagggtact taatgaaatc tcttaaatct
    12601 gaatttgcag aagtacatac gcttaataaa cgacgtgatc atttaccaat cacttcttta
    12661 tttaatcaat aatacaactt aataagccaa caatgatcca attagagaat atctttaatt
    12721 agatgttctc ttaactgtaa gcgaacttta ttttcaatga aatgtaatta attaagtaag
    12781 tgggactaaa acttttcttt tttagtccca tttttataaa caattattat tcattgttac
    12841 tactatcagc aattttttct ttttagtgaa acccaagtat ttgtaagcat gataaattac
    12901 ttctttttat atttattggg tttatttgta ttaatgtagt tggaatacat ctttaacaat
    12961 tcttctgttt catcaggact taaatgctca cattcaaaat acttttcaag cattgcacct
    13021 tttcgaatca gtctatgtgc tctttctttt ctatctaaat tttttaatct atgctttctt
    13081 aattctgctt tttcaatttc agcatttatt tttttgagat gtttaaaatc attcatcgta
    13141 tcacttacta ttctagttaa ttttgtcttc tgttaaattt tccttgttct cgttgctgct
    13201 attttgttcg atatctttat taaattctcc aatgacttta tccataattt tatacaattc
    13261 atcactagag ttgatcatat ttaaatcaag tttatttaaa aaataaccac caattttatt
    13321 agatagttgt tcttcttgtt ttttcttttg ctctttcagt tgtgcaattt gtttttctaa
    13381 ttgacttaat tttgtaatat ctgtttgttt aattttagac attaaaaaac tcccttagtt
    13441 tatctatata aagcatacca taattcattg ggtaagtcgc acatccatgg tataatataa
    13501 ccagttctta agacactgca ctgcccattt acatgcaaat tgaaaattgg cataaaaatg
    13561 ggcaacca
//
