LOCUS       NZ_CP073831            13570 bp    DNA     circular CON 17-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1220165 plasmid p1B1220165,
            complete sequence.
ACCESSION   NZ_CP073831
VERSION     NZ_CP073831.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699874
            Assembly: GCF_019329405.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 175x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019329405.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 06:59:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,679
            CDSs (total)                      :: 2,598
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 43 of 83
            Pseudo Genes (incomplete)         :: 45 of 83
            Pseudo Genes (internal stop)      :: 28 of 83
            Pseudo Genes (multiple problems)  :: 24 of 83
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073831.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1220165"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p1B1220165"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(13388..13570,1..384))
                     /gene="qacR"
                     /locus_tag="KFV27_RS13185"
                     /old_locus_tag="KFV27_13195"
     CDS             complement(join(13388..13570,1..384))
                     /gene="qacR"
                     /locus_tag="KFV27_RS13185"
                     /old_locus_tag="KFV27_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug-binding transcriptional regulator QacR"
                     /protein_id="WP_001807342.1"
                     /translation="MNLKDKILGVAKELFIKNGYNATTTGEIVKLSESSKGNLYYHFKT
                     KENLFLEILNIEESKWQEQWKKEQIKCKTNREKFYLYNELSLTTEYYYPLQNAIIEFYT
                     EYYKTNSINEKMNKLENKYIDAYHVIFKEGNLNGEWCINDVNAVSKIAANAVNGIVTFT
                     HEQNINERIKLMNKFSQIFLNGLSK"
                     /name="qacR"
     gene            563..2107
                     /gene="qac"
                     /locus_tag="KFV27_RS13190"
                     /old_locus_tag="KFV27_13200"
     CDS             563..2107
                     /gene="qac"
                     /locus_tag="KFV27_RS13190"
                     /old_locus_tag="KFV27_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            2312..2872
                     /locus_tag="KFV27_RS13195"
                     /old_locus_tag="KFV27_13205"
     CDS             2312..2872
                     /locus_tag="KFV27_RS13195"
                     /old_locus_tag="KFV27_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(3191..3952)
                     /locus_tag="KFV27_RS13205"
                     /old_locus_tag="KFV27_13215"
     CDS             complement(3191..3952)
                     /locus_tag="KFV27_RS13205"
                     /old_locus_tag="KFV27_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            4393..5328
                     /locus_tag="KFV27_RS13210"
                     /old_locus_tag="KFV27_13220"
     CDS             4393..5328
                     /locus_tag="KFV27_RS13210"
                     /old_locus_tag="KFV27_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            complement(5553..5792)
                     /locus_tag="KFV27_RS13215"
                     /old_locus_tag="KFV27_13225"
     CDS             complement(5553..5792)
                     /locus_tag="KFV27_RS13215"
                     /old_locus_tag="KFV27_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(5809..6078)
                     /locus_tag="KFV27_RS13220"
                     /old_locus_tag="KFV27_13230"
     CDS             complement(5809..6078)
                     /locus_tag="KFV27_RS13220"
                     /old_locus_tag="KFV27_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            6287..8209
                     /locus_tag="KFV27_RS13225"
                     /old_locus_tag="KFV27_13235"
     CDS             6287..8209
                     /locus_tag="KFV27_RS13225"
                     /old_locus_tag="KFV27_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            8483..8896
                     /locus_tag="KFV27_RS13525"
                     /old_locus_tag="KFV27_13240"
     CDS             8483..8896
                     /locus_tag="KFV27_RS13525"
                     /old_locus_tag="KFV27_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            8973..9206
                     /locus_tag="KFV27_RS13235"
                     /old_locus_tag="KFV27_13245"
     CDS             8973..9206
                     /locus_tag="KFV27_RS13235"
                     /old_locus_tag="KFV27_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(9255..9959)
                     /locus_tag="KFV27_RS13240"
                     /old_locus_tag="KFV27_13250"
     CDS             complement(9255..9959)
                     /locus_tag="KFV27_RS13240"
                     /old_locus_tag="KFV27_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(10110..10808)
                     /locus_tag="KFV27_RS13245"
                     /old_locus_tag="KFV27_13255"
     CDS             complement(10110..10808)
                     /locus_tag="KFV27_RS13245"
                     /old_locus_tag="KFV27_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            10972..11070
                     /locus_tag="KFV27_RS13250"
                     /old_locus_tag="KFV27_13260"
     CDS             10972..11070
                     /locus_tag="KFV27_RS13250"
                     /old_locus_tag="KFV27_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(11247..12158)
                     /locus_tag="KFV27_RS13255"
                     /old_locus_tag="KFV27_13265"
     CDS             complement(11247..12158)
                     /locus_tag="KFV27_RS13255"
                     /old_locus_tag="KFV27_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(12577..13137)
                     /locus_tag="KFV27_RS13260"
                     /old_locus_tag="KFV27_13270"
     CDS             complement(12577..13137)
                     /locus_tag="KFV27_RS13260"
                     /old_locus_tag="KFV27_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     ARG             564..2104
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          4394..5328
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6288..8140
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 atgataagca tctatatatt tgttttctaa tttattcatt ttttcattaa tgctattagt
       61 tttataatat tcagtgtaaa attcaattat tgcgttttga agtggataat agtattcggt
      121 tgttaaagat agttcattat ataaataaaa tttttctcta ttagttttac atttgatttg
      181 ttcctttttc cactgttctt gccatttaga ttcttctata tttaaaattt ctaaaaatag
      241 attttctttt gttttaaagt gataataaag attcccttta ctactttctg ataatttaac
      301 aatttctcca gtagtagtgg cattatatcc attttttata aataattcct ttgcgacacc
      361 tagtatttta tctttcaagt tcatcacgcc tttaatgagt attaagtact atcaatgata
      421 gcacaaaaaa ttcaaagaat aattgtataa cacaatacag ccatttaaat ttcgcaagat
      481 tttttgttgt aatatgtaaa aaaaatagat tataatcctt atagaccgat cgcacggtct
      541 ataaggattg gagggaactt aaatgatttc attttttaca aaaactactg atatgatgac
      601 atcaaaaaaa agatggactg cactagtagt attagctgtt agtttgtttg ttgttacaat
      661 ggatatgaca atattaatta tggctttacc ggaattagta agagagttag agccttctgg
      721 tacccaacag ttatggatag ttgatatata ctctcttgtt ttagctggct ttataattcc
      781 attgagtgcc tttgctgata aatggggaag aaaaaaagca ttattaactg gatttgcttt
      841 atttggcctc gtttcattag ctatattttt cgcagaaagt gcagagttcg taatagctat
      901 tcgattttta cttggtattg caggtgcttt aataatgcca actactcttt caatgataag
      961 agtaattttt gaaaacccta aagaaagggc cactgcatta gctgtatggt caatcgcttc
     1021 atcgataggt gctgtttttg gaccaattat cggaggagct ttacttgagc aattttcatg
     1081 gcactcggca tttttaatta atgtaccgtt tgcgataata gcagttgtag caggtttatt
     1141 tttattacca gagtctaagt tatcaaaaga aaagtctcac tcgtgggata ttccttctac
     1201 aattttatca attgcaggca tgattggact ggtatggagt atcaaagaat tttcaaaaga
     1261 aggactagca gatattattc catgggttgt aatagtatta gcaattacca tgatagtgat
     1321 atttgttaaa cgtaatttat caagttctga tccaatgtta gacgtaagac tttttaaaaa
     1381 gagatcattt tcagctggta caattgctgc atttatgaca atttttgcaa tggcatctgt
     1441 tttgttatta gcttcacaat ggttacaggt tgtggaagaa ctttctcctt ttaaagctgg
     1501 cttataccta ttacctatgg caataggaga tatggtgttt gcaccaattg cacccggatt
     1561 agcggcgcga tttggaccga aaatagtgtt accttccgga attggaattg cagccattgg
     1621 catgtttatt atgtatttct ttggtcatcc attatcatat tctacaatgg ctttagcatt
     1681 aattttagtt ggagctggta tggcttcact agcagttgca tctgctctaa taatgttaga
     1741 aacacctaca tcaaaagcag gtaatgcagc tgctgttgaa gagtctatgt atgaccttgg
     1801 aaatgttttt ggtgtagcag tacttggtag cctatcttct atgctttatc gtgtattttt
     1861 agatatttca tctttttcat caaaaggtat agttggagat ttagctcatg tagctgaaga
     1921 atctgtagtg ggcgctgtcg aagtagctaa agctacgggg ataaaacagc ttgcaaacga
     1981 ggctgtaaca tcatttaatg atgcttttgt agcaactgct ttagtaggtg ggattatcat
     2041 gattatcatt tcaatagttg tctatttgtt aattcccaaa tcacttgata taactaaaca
     2101 aaaatagtac taaacatata tttttagata ttgtagatta gtttattttt aaaattagta
     2161 atagttaaaa agtagaacca ttaattttaa atggttctac ttttttatta ttaagtaaaa
     2221 ttataaaaga caatttctta aattaataat atgatatacc tatatctaat aatttattag
     2281 tattaataaa ccttgaaagg atgattaact gatgaataga gcagatattt ttaaaatatt
     2341 tgatgtagaa cttaatataa ctatattttt aggagcacta gtagcaactt tcatctattt
     2401 tataagttac atgaacaatg taaatgaatt aattacatta tgcttaacat tagcttttcc
     2461 tgcatattta ttaaaaatta gcagatcgtt cgaggttatt ttattagagg aaaaaataaa
     2521 aaatagtaaa atcaaaatct gtgacaaaga tagaattgaa cgagagatac tggtgaaaaa
     2581 gaacctaata gatgaactat tgtttcctaa tagagttatt aaaaaatata cactgacaat
     2641 tttaatttac agtataggta ttattattct tttactactt tgtaatatgt taagttatgg
     2701 aattggtgaa aatgtcaatt ttatctggag attactagct tgttatgtaa cgagtgtcac
     2761 agtgattata gttgccttga ttataatgat aatgatattt gatcttataa ttggattact
     2821 attaaatttt caaatatttt taaaaaataa aaagtatgat gattttttat aattattcga
     2881 tttatataat gaaaaattaa atgattaaac atagaaacct caaaacaaac catatttaat
     2941 agagtttatg gcgccttata caaataaagg ctatgctgtt aaagaattat gtagctatct
     3001 tcagattaat atggatgatg tttatactat tggcgataga aaattgattt acctatgttt
     3061 aataccctag ataattcttc tgataattta gtttttgtta gagtttaaca taactgatga
     3121 ttatcagaag tttgattcac attaaaaagg cacaacaagt tcatgaactt gtgtgccttt
     3181 ttaattgcaa ttagttacca aataaacgac tccaaaatct ctttttaggt ggttctacct
     3241 ccctattatc actggaatcg ttcttcgttt tttcaacaaa cttatcctct tttgttaatt
     3301 catctgtact gtcatgctta ttctcttcag ttatattttt atgatttact tcggttggtt
     3361 gacattcttt ttgttgttgg ttgatatttt gagattcttc agtaaaagtt gcttcttgtg
     3421 gattaatatt ctgtctatta ttcatcgttg tatcaaaaga atatttaagc tgtctttctt
     3481 cttctaattg gctttctagt ttttgaattt ttttattgtt ttctaaagat aatatttgtt
     3541 gattctccaa cagtttattt aatgtattaa cattgctatt ttgttgatct aattgtttag
     3601 ttagattctc aatatatttt tcatcatttt tagctctagt ttcaaagatt tctatttgct
     3661 tttctaattc gtttactttt gtttttaatg tctcagaact atttgaatta ttatgtatat
     3721 acttttcaac ttttgcagta ttttcattaa ataattcatt ttttactgat tctttattat
     3781 gagttgtttt ctctttctta gtgatttttt ctataatttt taaataatca ttatctttaa
     3841 tataattaat tccattttct ttttcaaaag atatatttaa tcttttagca ttattaacaa
     3901 tagtttgttt agttacacct aattcatcag caatcatttt aatagttttc ataacatccc
     3961 cccttaggta cctaatttag tacctaaatt ttaccaccta acaaaattta tttcatcatt
     4021 tagtactaaa tttactatct attaactacc taaatgccta attatctatc cttaaaatat
     4081 tttcgccact taattaggtg ctaaaatagc gaattatacg tttggtagtt ttaggtgtac
     4141 ttttaattac atttaaaact ctttatatac gccattaaaa gtgttaatat tactttataa
     4201 atattaaaag agtcgatgct attggcgtag catcgactct cggtaataaa acgattcgca
     4261 ctcgtttgtt tatatatttt tttgatactt gtattatata tatctaatca tctaagtgca
     4321 agcacaaaac atataactta cgtaaaaatt gttttattac ctcaatccca aaatggaaat
     4381 gaggttttta ttatgcccaa ttttgaaaaa tataatttat cacaagtaaa aactgaaaga
     4441 ttttatcaac tgcctaaata tttatttgaa gatgcatatt ttaagaaaat gtctgcagaa
     4501 gccaaaatta tgtatgcgtt attaaaagat cgttttgaat tatccctcca aaatgaatgg
     4561 gtagataaaa ataataatat ttactttatt ttcagtaata aacatttgtg tgaatactta
     4621 ggttatgcag aacaaaaaat tataaaatta aaaaaagagt taataaaatt taatttacta
     4681 actcaagaac gtgttggcct taataaacca aatagattat acctattaaa acctaattat
     4741 gacattgaag ccagtcatat caaggaactt ccaaattcac agttccagaa caatgaattt
     4801 ggaagttcta gaactgtgaa tttaagtggt caagaacttc caaattcaca gtctaatgat
     4861 actgattata atgacactga ttatattaag actaattata atgatatgta tgatttgaat
     4921 gataggaaat taacatattc tagtaatcat acaaatcatt caaatcacta ctattcaaaa
     4981 ttcaatgatg aagctttaaa atttcaatta cttgaagaac taccacaaag tattcaaagt
     5041 tatttaagta acttttctgt atctgaaatt aaacttatta aatctgtgtt attaaaagcc
     5101 aaaacatcct tcaacaattc tattgatgca tattatttat tagaagatat ggaatttgaa
     5161 attgttaatg ttcttaagcg ttttaaagct acattaattc aaaaaaatga aaccgttgaa
     5221 gcaatgcaag ggtacttaat gaaatctctt aaatctgaat ttgcagaagt acatacgctt
     5281 aataaacgac gtgatcattt accaatcact tctttattta atcaataata caacttaata
     5341 agccaacaat gatccaatta gagaatatct ttaattagat gttctcttaa ctgtaagcga
     5401 actttatttt caatgaaatg taattaatta agtaagtggg actaaaactt ttctttttta
     5461 gtcccatttt tataaacaat tattattcat tgttactact atcagcaatt ttttcttttt
     5521 agtgaaaccc aagtatttgt aagcatgata aattacttct ttttatattt attgggttta
     5581 tttgtattaa tgtagttgga atacatcttt aacaattctt ctgtttcatc aggacttaaa
     5641 tgctcacatt caaaatactt ttcaagcatt gcaccttttc gaatcagtct atgtgctctt
     5701 tcttttctat ctaaattttt taatctatgc tttcttaatt ctgctttttc aatttcagca
     5761 tttatttttt tgagatgttt aaaatcattc atcgtatcac ttactattct agttaatttt
     5821 gtcttctgtt aaattttcct tgttctcgtt gctgctattt tgttcgatat ctttattaaa
     5881 ttctccaatg actttatcca taattttata caattcatca ctagagttga tcatatttaa
     5941 atcaagttta tttaaaaaat aaccaccaat tttattagat agttgttctt cttgtttttt
     6001 cttttgctct ttcagttgtg caatttgttt ttctaattga cttaattttg taatatctgt
     6061 ttgtttaatt ttagacatta aaaaactccc ttagtttatc tatataaagc ataccataat
     6121 tcattgggta agtcgcacat ccatggtata atataaccag ttcttaagac actgcactgc
     6181 ccatttacat gcaaattgaa aattggcata aaaatgggca accatactgg ttgatcatta
     6241 tagttcccac aggggtaaaa aaacagaaaa gagaggaggt aaaatcatga ataaaggtta
     6301 ctttatgttg agagtgacta acattaatcg agcaacaaaa aatatcgttg cgtcagcgtc
     6361 ttatcgttct gatgaagcat tatatagcga acgtacagat gaaaaaatta agtttagaaa
     6421 tcatactatt aagcctgaaa gcatgatttt aactcctcaa catgcacccg aatggactaa
     6481 agatagacag cgtttatgga atgaagttga taaagttgaa aagaataatg cgaaaacaaa
     6541 aaatcctaga ttagcaaaag aagtcttatt aagtttgcct aatgattttg atagagaggt
     6601 acaaactgaa ttaactaaag attttataaa aagtgagttt gtagacaaag gaatggttgc
     6661 tgatatttca attcatagag atgatataaa caacccacac gcccatgttc tacttactca
     6721 aagaccgttt aatgcagacg gtacttgggg gaaaaagaca aaaacgagaa cgcaatatga
     6781 tgaaaatggc aatgcgattt taaataaaaa tggtaataaa gttagaaaac aagaacgatt
     6841 tgccgacttt gatttcaaag aagttcgtaa gcattgggaa ttaaaattaa atcaatactc
     6901 agaacgtgaa caaagtttaa gacgatatga tagtcgttct tttgaagcgc aaggtttaga
     6961 aaaaattgcc gaaattccac ttacaagaga agaatataga ttagaacaaa atgaaagaaa
     7021 acgctgtgaa aaagagggta tcgagtacca acccgttact tattacggac agaaaaatga
     7081 acaaattaga agatataatc gtggcgaagt atcacaatta tttagtgata aagagaaaga
     7141 acaagcacaa aatgaaatta gattatttat tcaaaatgct aacgaacaag taagtaaaaa
     7201 tgaacaaagt tattctattt tatttgatag atatcaacgt gatgttggct attcagaggc
     7261 gaaagaaaca attaaaaata cgtttgacag tgcgtctacc tttggtcgta aaattcaaaa
     7321 tgatttactt aaaaatgaaa ttaagagacg tcaattaaaa ttctatgcta aaaattatga
     7381 tgaaaattat cagtttagaa aagaaattga aagtaaagga tatagtcaac aagagtttgt
     7441 taatactgtt gaaactaact ataaagcgtt actcaatgag aagaataaat tagaaagtag
     7501 agaggaaaaa cgtaaagaga tttatcaatc agctaaagat gtttatctaa ctgaaagtga
     7561 aaaaaatcat ctcattgttc agcaaatgta ccctaaatct catcatcatt ttagccatga
     7621 tgaaaaagca tttattgttg atgaggcaca aaaaggtaat attataaaag caactcatgt
     7681 tcaatacctg ttcaaacaaa ataacaatat tcctaatgat gtattgctag aagatactta
     7741 taaaaaagcg agtaaagata ttttctttag tcaaaaacat cttaaaaatt tagaaaaaga
     7801 tagctttgaa tataacgttg agttgcaatt tattaaggaa aaagaaaaag aaattgatag
     7861 ttataaagaa catattgatg atgatttaag aatagatata ggcgataaaa actttgaaaa
     7921 agtcaaagac caaacaccta atgaaaaagt tcaattactc attaaacttg agaaatataa
     7981 tcatgtcgat caagaagaag ttatcgaaaa acatattaat caagtagata gaaatgatca
     8041 tgacaatcgt attgaacatg aaaatgaaca aactaatcac attaatttac caacgataaa
     8101 tgctagtgac tttcttatgt actatgcgaa cgaaactgaa aagcaaaaca atattaatca
     8161 tcagaaaaag aagaagaaac aagagagaaa taaccaaatg agaagatagt aagcagttga
     8221 aatcatacaa agttcattct ttaataataa gaaacttaac ccaatgtggt aaattagctt
     8281 tggtttaaag aaaacattta tatacttaga taaggtacat tctatgtatt atcacaatgt
     8341 attttaaaac gtgtataatt atagaaaaac cttgatatta tgcgattgtt cacatatttc
     8401 agcataaaaa atactaatgt tcataaatat cgatagaaaa aattgagttt aaatatatct
     8461 taaagttaag gagaaaaata atttggaaac aaaaattgga catcaaatac aaaagttaag
     8521 aaaaagtaaa aacatgtctc aagaaaaatt agctgaaaag ttaggggtat cgagacattc
     8581 tatttctaat tgggaaagag aagtaagtaa tcccgactta aaaacaattt tagaaatcac
     8641 taaattattt aatgtttctc taaatcaatt aattaaagga gtggaaatca tgcaagttaa
     8701 taaatatgtt tactctgcat tagcattttt tctaggaggt ttcggggctc ataagtttta
     8761 tgttaagaaa aataaaaatg ctcttcttta tttactattt tgttggacgg gaataccggg
     8821 tgtgataggt atggtagaag gtgttttaac acttatgcgt ccgagcgata gtgaaaacaa
     8881 tatagaaata aattaaacca aaaataaaaa gccgatgtat cggatttttt ttagtgtaca
     8941 tgtgaatatt aaaaatgatg gagtgatttc acatgaacac attcgttttc ggcgtgatta
     9001 caaagcgatt agaagaaata ggttttaatg tacacaaggt gtttaaaatg cccgaaaaac
     9061 aggcactatt tgaagatttg taccattatg tgagatacgt taaaccatgc aaggctgaac
     9121 taacaatggt ggcattagat agtgatgaat tgattcatta ctttatatgc tgttacatgg
     9181 aacgttatgc ttatcttttg aactaatgat aaaaaagacg cctaatttta ggcgcctttt
     9241 taataatcta ctaactactt attatatcta attttacttg ctaacttagc tactgtttta
     9301 ttgtcgtagg gtttaatata gactgtttca atattctcct ctattgcttt ttttgttatc
     9361 ttattcattt gactataatc actattttgt gaattttccc atttataagg gatattatta
     9421 tatacattaa ttgttccatc accattactg ctatatgtta cagacccacc agctctcata
     9481 ggagcttcta atttcacaac tttttcttta taaattgcac tatttttggc ttgtggattt
     9541 acttttgagc cttttggtat ttctgtaaca taaacatttt taataccttt taaatcattt
     9601 cttgtagata ttaactgata ccaaactcgt gcatattcaa tttctttcga gttaaaatta
     9661 tttaagtaac ttttattttt atcagtagat tgttgcttct tattactatc attatttttt
     9721 tcttgttttt tattatcatt tttttcttta ttttgtttat tactactttc tgaattatcc
     9781 gcactatcag atgtgttagt tttttcgtca atttcatttg atgcttcatt atctttgttt
     9841 tctgaggtgt tagtatcact agaattatta tcattgtgat gttctgaatc gttattatta
     9901 caactcacta aaattaccga taaactaaca attaatatag gtaaaatgat ttttttcata
     9961 ttacaactcc ttttcagtat ttcaaattag attatgttaa tattttttca gcaagaaaac
    10021 gtaaattaca tgagttgaaa aatatgtatt aaatatataa taaaataacc acatgtttta
    10081 cataaacacg gtggttattt tatcaatttt tatattgcct tttttctgaa tattaaaaga
    10141 cttataccta atattagtag tccagaagcg ccaattattg acaatatatc actttggttg
    10201 ctttctcctg tttctggtaa tacttgagag tttgaatctt gatttttcat tccttcagaa
    10261 ctcttgtttt tattttcagt tacaggtgtc aacacacctt gaggtgtatt aatagtttta
    10321 ttttcttcat tagctatctt ttgactttca ttttttacaa tttgattata tttttctgtg
    10381 gatacactat tagggttagg aacatgactt tctaattctt ggtattcttc ttgagtcata
    10441 caattaggta tcacccctcc gaaaggtctt ttaccttgtt gagttctctc attattttgt
    10501 atatcagtac tttgtgattg atattcatta aatacacttg aaactttctc attatactcg
    10561 atttgagtta attcccctga atctttctgt ttagaaagtt cacttatttg attttgtgca
    10621 gattgtggga gggatgaagt attgaagtct acattagccc aaccatctcc atttgtatct
    10681 gcgctatttg gtaagttctc tccgtatacg gttttggcac catcctcagc attggctgtc
    10741 gctgaaaaac ctgtgaatgt taatgagcta gccaataata aagttggaag taatgatcgt
    10801 tttttcataa aattatctcc tttaatagaa tttgtatttt cattatcaca tagatacatt
    10861 aaacataaaa tgacttatcg gaagtgcata tgcttaacaa ttcacaaatg atataatata
    10921 aaagtcgtcg ttctacttct gtgctggttt agcatggaag gaggtgacac catgttcagc
    10981 atcatatttg tatctatagt agctcctatt atagtagggg ttataatcac gttgttttca
    11041 cattggttga ataatcgtga taaacaataa agcgacgaca cagtaataac accaaaaaga
    11101 gcccgataac tactggaata gttatcgggc tcggtgttta ccatgttcaa cacatatttg
    11161 ttatctaaat tataacatca tagtgtggag gaatgcaaaa ttgaataatt agtatgttaa
    11221 gtctatacta tactgattag taagtactaa ttatcatctt tgttattgtt acccctttca
    11281 ttggcttttg aatctaattc tttttgaaat tttttgtgtg attttgaaga agtgtttaaa
    11341 aagaaatagg tcatattatc catacgatgt ccttttataa attcattgat aaattgtttt
    11401 tttgtcattt ttttggttgt attacgttgt ttttgcaatc taatgatttc ttcttcaatg
    11461 tatactctta tcatcaaatc taaactagat gtctgattat aagtagtgaa taattttgga
    11521 actaataaag ttttattacc atcaaaaaga aatggagtga aatcataagt ttcccatata
    11581 ccgttagtag agaaataata atatgtcaca gtttctttag aaagaaacga tttcatttct
    11641 ggaaatgact gaatcatgga tttattaaaa tcaattataa cattcatgat tatattactt
    11701 gttaaatctg ataatttatc atcaccaaaa tttctgatta atggtgcttt ttgaggtatt
    11761 gttaatccgt catcatttaa gataatggaa agaatatctt cattcataaa tagattaatc
    11821 aattcagatg ctgtagtacc ttttcctgta ttacctttct gagaaaaacc taagcgtaaa
    11881 tggtgtggtt ctgaaaaatg agagaataat tttctaaatt cattacgctt ttgggtatca
    11941 gaaaaatcac taagatgtat atcgataatt ttttcaaaaa aaacatcaat ttgctcttgt
    12001 gctctttgag ccaaagtcgc aacttttcca acttctctat tagatattac tttaataagg
    12061 tttgggtcta tatattgaag agtgtcttct tcaatagaaa tatttaaata atcagttgat
    12121 ttagcatcat gattgaattt ggtacatact agtgtcactc tatatcatcc ttctgtaagt
    12181 tttttgttta cttccaatat agaatgatgt attattgtaa ttgtcgattt acaataagtt
    12241 gttattctag gtggaagttt attgcttttg atgtatagat agacttaacc ttttcattat
    12301 agcatactat agaagaaaat gcaagcaaaa gcatataaac aacaaatgtt gtttatatgc
    12361 ttttgctttt ttgttaattg gagaggacaa ggtgaatatt aacagtcaaa atccagttat
    12421 gtcggttaat gaatcgatga aaattttcaa agctcaagta atgcttagaa atttaaagct
    12481 attaagcgag tggattgtta aaggggactg aagttaaaga cactttttaa gaagattttt
    12541 caattaaagg gtatattaaa actttaattt agtactttat atttttcttg actctttaat
    12601 aatcctatat acagtagaac gactcactcc tgtttgttca gcaatttctt caccagacag
    12661 cttttgttca tcatataaga atttgatctc tcttttctta tgatctggta gtgaaggtct
    12721 tccacctttt ctccctcttg ctcttgcagc ttccaaacct tttttagttc gttcacttaa
    12781 taaattagct tcaagttcag cgaatgcact catcatggta aagaacatct tgcccatcgc
    12841 atctttcgtt gacacattca tatctataat atgtaaatca atgctatttt catccaacca
    12901 ttgagatagc tcaattaatt gttttgttgt acgtccaaga cgatctaatt tatagacaac
    12961 tagaatatct ccttcacgta aataatctaa acacttatca agttccgttc ttttagtttt
    13021 acgaccactc gctttctcgc taaagatacg ttcacaacca tattcctcaa gtgtatcaat
    13081 ctgtccatca agactttgat cttgtgttga cacacgtgca taaccaattt tagccatttg
    13141 aacaccacct tttttaatcg ttttatgggt acttactaat gaaatatagt tctcgatgta
    13201 aataaacacg tctgtcagca tctaaaaact catgattttc gatatttatg atacattgat
    13261 tataacacaa gtttatagac ttaaatatcc aagaaaaacc tcttaatatt aagttaagag
    13321 gttttgtgtc aaaaacgaac gtaatttaga aataatattt attggtattt caagaatata
    13381 tatataatta tttactaagt ccatttaaaa aaatttgaga gaacttgttc ataagtttaa
    13441 ttctttcatt aatattttgt tcatgtgtaa aggtaacaat tccattaaca gcgtttgctg
    13501 ctattttact aacagcatta acatcattaa tacaccattc gccatttaaa ttaccctcct
    13561 taaaaattac
//
