LOCUS       NZ_CP073828            13570 bp    DNA     circular CON 17-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1208538 plasmid p1B1208538,
            complete sequence.
ACCESSION   NZ_CP073828
VERSION     NZ_CP073828.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699873
            Assembly: GCF_019329365.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 368x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019329365.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 06:48:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,690
            CDSs (total)                      :: 2,609
            Genes (coding)                    :: 2,525
            CDSs (with protein)               :: 2,525
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 47 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 24 of 84
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073828.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1208538"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p1B1208538"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(13373..13570,1..42))
                     /locus_tag="KFV36_RS13220"
                     /old_locus_tag="KFV36_13230"
     CDS             complement(join(13373..13570,1..42))
                     /locus_tag="KFV36_RS13220"
                     /old_locus_tag="KFV36_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(59..328)
                     /locus_tag="KFV36_RS13225"
                     /old_locus_tag="KFV36_13235"
     CDS             complement(59..328)
                     /locus_tag="KFV36_RS13225"
                     /old_locus_tag="KFV36_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            537..2459
                     /locus_tag="KFV36_RS13230"
                     /old_locus_tag="KFV36_13240"
     CDS             537..2459
                     /locus_tag="KFV36_RS13230"
                     /old_locus_tag="KFV36_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            2733..3146
                     /locus_tag="KFV36_RS13575"
                     /old_locus_tag="KFV36_13245"
     CDS             2733..3146
                     /locus_tag="KFV36_RS13575"
                     /old_locus_tag="KFV36_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            3223..3456
                     /locus_tag="KFV36_RS13240"
                     /old_locus_tag="KFV36_13250"
     CDS             3223..3456
                     /locus_tag="KFV36_RS13240"
                     /old_locus_tag="KFV36_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(3505..4209)
                     /locus_tag="KFV36_RS13245"
                     /old_locus_tag="KFV36_13255"
     CDS             complement(3505..4209)
                     /locus_tag="KFV36_RS13245"
                     /old_locus_tag="KFV36_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(4360..5058)
                     /locus_tag="KFV36_RS13250"
                     /old_locus_tag="KFV36_13260"
     CDS             complement(4360..5058)
                     /locus_tag="KFV36_RS13250"
                     /old_locus_tag="KFV36_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            5222..5320
                     /locus_tag="KFV36_RS13255"
                     /old_locus_tag="KFV36_13265"
     CDS             5222..5320
                     /locus_tag="KFV36_RS13255"
                     /old_locus_tag="KFV36_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(5497..6408)
                     /locus_tag="KFV36_RS13260"
                     /old_locus_tag="KFV36_13270"
     CDS             complement(5497..6408)
                     /locus_tag="KFV36_RS13260"
                     /old_locus_tag="KFV36_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(6827..7387)
                     /locus_tag="KFV36_RS13265"
                     /old_locus_tag="KFV36_13275"
     CDS             complement(6827..7387)
                     /locus_tag="KFV36_RS13265"
                     /old_locus_tag="KFV36_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            complement(7638..8204)
                     /gene="qacR"
                     /locus_tag="KFV36_RS13270"
                     /old_locus_tag="KFV36_13280"
     ARG             complement(7642..8204)
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            8383..9927
                     /gene="qac"
                     /locus_tag="KFV36_RS13275"
                     /old_locus_tag="KFV36_13285"
     CDS             8383..9927
                     /gene="qac"
                     /locus_tag="KFV36_RS13275"
                     /old_locus_tag="KFV36_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            10132..10692
                     /locus_tag="KFV36_RS13280"
                     /old_locus_tag="KFV36_13290"
     CDS             10132..10692
                     /locus_tag="KFV36_RS13280"
                     /old_locus_tag="KFV36_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(11011..11772)
                     /locus_tag="KFV36_RS13290"
                     /old_locus_tag="KFV36_13300"
     CDS             complement(11011..11772)
                     /locus_tag="KFV36_RS13290"
                     /old_locus_tag="KFV36_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            12213..13148
                     /locus_tag="KFV36_RS13295"
                     /old_locus_tag="KFV36_13305"
     CDS             12213..13148
                     /locus_tag="KFV36_RS13295"
                     /old_locus_tag="KFV36_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     ARG             8384..9924
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          12214..13148
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          538..2390
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 aatttcagca tttatttttt tgagatgttt aaaatcattc atcgtatcac ttactattct
       61 agttaatttt gtcttctgtt aaattttcct tgttctcgtt gctgctattt tgttcgatat
      121 ctttattaaa ttctccaatg actttatcca taattttata caattcatca ctagagttga
      181 tcatatttaa atcaagttta tttaaaaaat aaccaccaat tttattagat agttgttctt
      241 cttgtttttt cttttgctct ttcagttgtg caatttgttt ttctaattga cttaattttg
      301 taatatctgt ttgtttaatt ttagacatta aaaaactccc ttagtttatc tatataaagc
      361 ataccataat tcattgggta agtcgcacat ccatggtata atataaccag ttcttaagac
      421 actgcactgc ccatttacat gcaaattgaa aattggcata aaaatgggca accatactgg
      481 ttgatcatta tagttcccac aggggtaaaa aaacagaaaa gagaggaggt aaaatcatga
      541 ataaaggtta ctttatgttg agagtgacta acattaatcg agcaacaaaa aatatcgttg
      601 cgtcagcgtc ttatcgttct gatgaagcat tatatagcga acgtacagat gaaaaaatta
      661 agtttagaaa tcatactatt aagcctgaaa gcatgatttt aactcctcaa catgcacccg
      721 aatggactaa agatagacag cgtttatgga atgaagttga taaagttgaa aagaataatg
      781 cgaaaacaaa aaatcctaga ttagcaaaag aagtcttatt aagtttgcct aatgattttg
      841 atagagaggt acaaactgaa ttaactaaag attttataaa aagtgagttt gtagacaaag
      901 gaatggttgc tgatatttca attcatagag atgatataaa caacccacac gcccatgttc
      961 tacttactca aagaccgttt aatgcagacg gtacttgggg gaaaaagaca aaaacgagaa
     1021 cgcaatatga tgaaaatggc aatgcgattt taaataaaaa tggtaataaa gttagaaaac
     1081 aagaacgatt tgccgacttt gatttcaaag aagttcgtaa gcattgggaa ttaaaattaa
     1141 atcaatactc agaacgtgaa caaagtttaa gacgatatga tagtcgttct tttgaagcgc
     1201 aaggtttaga aaaaattgcc gaaattccac ttacaagaga agaatataga ttagaacaaa
     1261 atgaaagaaa acgctgtgaa aaagagggta tcgagtacca acccgttact tattacggac
     1321 agaaaaatga acaaattaga agatataatc gtggcgaagt atcacaatta tttagtgata
     1381 aagagaaaga acaagcacaa aatgaaatta gattatttat tcaaaatgct aacgaacaag
     1441 taagtaaaaa tgaacaaagt tattctattt tatttgatag atatcaacgt gatgttggct
     1501 attcagaggc gaaagaaaca attaaaaata cgtttgacag tgcgtctacc tttggtcgta
     1561 aaattcaaaa tgatttactt aaaaatgaaa ttaagagacg tcaattaaaa ttctatgcta
     1621 aaaattatga tgaaaattat cagtttagaa aagaaattga aagtaaagga tatagtcaac
     1681 aagagtttgt taatactgtt gaaactaact ataaagcgtt actcaatgag aagaataaat
     1741 tagaaagtag agaggaaaaa cgtaaagaga tttatcaatc agctaaagat gtttatctaa
     1801 ctgaaagtga aaaaaatcat ctcattgttc agcaaatgta ccctaaatct catcatcatt
     1861 ttagccatga tgaaaaagca tttattgttg atgaggcaca aaaaggtaat attataaaag
     1921 caactcatgt tcaatacctg ttcaaacaaa ataacaatat tcctaatgat gtattgctag
     1981 aagatactta taaaaaagcg agtaaagata ttttctttag tcaaaaacat cttaaaaatt
     2041 tagaaaaaga tagctttgaa tataacgttg agttgcaatt tattaaggaa aaagaaaaag
     2101 aaattgatag ttataaagaa catattgatg atgatttaag aatagatata ggcgataaaa
     2161 actttgaaaa agtcaaagac caaacaccta atgaaaaagt tcaattactc attaaacttg
     2221 agaaatataa tcatgtcgat caagaagaag ttatcgaaaa acatattaat caagtagata
     2281 gaaatgatca tgacaatcgt attgaacatg aaaatgaaca aactaatcac attaatttac
     2341 caacgataaa tgctagtgac tttcttatgt actatgcgaa cgaaactgaa aagcaaaaca
     2401 atattaatca tcagaaaaag aagaagaaac aagagagaaa taaccaaatg agaagatagt
     2461 aagcagttga aatcatacaa agttcattct ttaataataa gaaacttaac ccaatgtggt
     2521 aaattagctt tggtttaaag aaaacattta tatacttaga taaggtacat tctatgtatt
     2581 atcacaatgt attttaaaac gtgtataatt atagaaaaac cttgatatta tgcgattgtt
     2641 cacatatttc agcataaaaa atactaatgt tcataaatat cgatagaaaa aattgagttt
     2701 aaatatatct taaagttaag gagaaaaata atttggaaac aaaaattgga catcaaatac
     2761 aaaagttaag aaaaagtaaa aacatgtctc aagaaaaatt agctgaaaag ttaggggtat
     2821 cgagacattc tatttctaat tgggaaagag aagtaagtaa tcccgactta aaaacaattt
     2881 tagaaatcac taaattattt aatgtttctc taaatcaatt aattaaagga gtggaaatca
     2941 tgcaagttaa taaatatgtt tactctgcat tagcattttt tctaggaggt ttcggggctc
     3001 ataagtttta tgttaagaaa aataaaaatg ctcttcttta tttactattt tgttggacgg
     3061 gaataccggg tgtgataggt atggtagaag gtgttttaac acttatgcgt ccgagcgata
     3121 gtgaaaacaa tatagaaata aattaaacca aaaataaaaa gccgatgtat cggatttttt
     3181 ttagtgtaca tgtgaatatt aaaaatgatg gagtgatttc acatgaacac attcgttttc
     3241 ggcgtgatta caaagcgatt agaagaaata ggttttaatg tacacaaggt gtttaaaatg
     3301 cccgaaaaac aggcactatt tgaagatttg taccattatg tgagatacgt taaaccatgc
     3361 aaggctgaac taacaatggt ggcattagat agtgatgaat tgattcatta ctttatatgc
     3421 tgttacatgg aacgttatgc ttatcttttg aactaatgat aaaaaagacg cctaatttta
     3481 ggcgcctttt taataatcta ctaactactt attatatcta attttacttg ctaacttagc
     3541 tactgtttta ttgtcgtagg gtttaatata gactgtttca atattctcct ctattgcttt
     3601 ttttgttatc ttattcattt gactataatc actattttgt gaattttccc atttataagg
     3661 gatattatta tatacattaa ttgttccatc accattactg ctatatgtta cagacccacc
     3721 agctctcata ggagcttcta atttcacaac tttttcttta taaattgcac tatttttggc
     3781 ttgtggattt acttttgagc cttttggtat ttctgtaaca taaacatttt taataccttt
     3841 taaatcattt cttgtagata ttaactgata ccaaactcgt gcatattcaa tttctttcga
     3901 gttaaaatta tttaagtaac ttttattttt atcagtagat tgttgcttct tattactatc
     3961 attatttttt tcttgttttt tattatcatt tttttcttta ttttgtttat tactactttc
     4021 tgaattatcc gcactatcag atgtgttagt tttttcgtca atttcatttg atgcttcatt
     4081 atctttgttt tctgaggtgt tagtatcact agaattatta tcattgtgat gttctgaatc
     4141 gttattatta caactcacta aaattaccga taaactaaca attaatatag gtaaaatgat
     4201 ttttttcata ttacaactcc ttttcagtat ttcaaattag attatgttaa tattttttca
     4261 gcaagaaaac gtaaattaca tgagttgaaa aatatgtatt aaatatataa taaaataacc
     4321 acatgtttta cataaacacg gtggttattt tatcaatttt tatattgcct tttttctgaa
     4381 tattaaaaga cttataccta atattagtag tccagaagcg ccaattattg acaatatatc
     4441 actttggttg ctttctcctg tttctggtaa tacttgagag tttgaatctt gatttttcat
     4501 tccttcagaa ctcttgtttt tattttcagt tacaggtgtc aacacacctt gaggtgtatt
     4561 aatagtttta ttttcttcat tagctatctt ttgactttca ttttttacaa tttgattata
     4621 tttttctgtg gatacactat tagggttagg aacatgactt tctaattctt ggtattcttc
     4681 ttgagtcata caattaggta tcacccctcc gaaaggtctt ttaccttgtt gagttctctc
     4741 attattttgt atatcagtac tttgtgattg atattcatta aatacacttg aaactttctc
     4801 attatactcg atttgagtta attcccctga atctttctgt ttagaaagtt cacttatttg
     4861 attttgtgca gattgtggga gggatgaagt attgaagtct acattagccc aaccatctcc
     4921 atttgtatct gcgctatttg gtaagttctc tccgtatacg gttttggcac catcctcagc
     4981 attggctgtc gctgaaaaac ctgtgaatgt taatgagcta gccaataata aagttggaag
     5041 taatgatcgt tttttcataa aattatctcc tttaatagaa tttgtatttt cattatcaca
     5101 tagatacatt aaacataaaa tgacttatcg gaagtgcata tgcttaacaa ttcacaaatg
     5161 atataatata aaagtcgtcg ttctacttct gtgctggttt agcatggaag gaggtgacac
     5221 catgttcagc atcatatttg tatctatagt agctcctatt atagtagggg ttataatcac
     5281 gttgttttca cattggttga ataatcgtga taaacaataa agcgacgaca cagtaataac
     5341 accaaaaaga gcccgataac tactggaata gttatcgggc tcggtgttta ccatgttcaa
     5401 cacatatttg ttatctaaat tataacatca tagtgtggag gaatgcaaaa ttgaataatt
     5461 agtatgttaa gtctatacta tactgattag taagtactaa ttatcatctt tgttattgtt
     5521 acccctttca ttggcttttg aatctaattc tttttgaaat tttttgtgtg attttgaaga
     5581 agtgtttaaa aagaaatagg tcatattatc catacgatgt ccttttataa attcattgat
     5641 aaattgtttt tttgtcattt ttttggttgt attacgttgt ttttgcaatc taatgatttc
     5701 ttcttcaatg tatactctta tcatcaaatc taaactagat gtctgattat aagtagtgaa
     5761 taattttgga actaataaag ttttattacc atcaaaaaga aatggagtga aatcataagt
     5821 ttcccatata ccgttagtag agaaataata atatgtcaca gtttctttag aaagaaacga
     5881 tttcatttct ggaaatgact gaatcatgga tttattaaaa tcaattataa cattcatgat
     5941 tatattactt gttaaatctg ataatttatc atcaccaaaa tttctgatta atggtgcttt
     6001 ttgaggtatt gttaatccgt catcatttaa gataatggaa agaatatctt cattcataaa
     6061 tagattaatc aattcagatg ctgtagtacc ttttcctgta ttacctttct gagaaaaacc
     6121 taagcgtaaa tggtgtggtt ctgaaaaatg agagaataat tttctaaatt cattacgctt
     6181 ttgggtatca gaaaaatcac taagatgtat atcgataatt ttttcaaaaa aaacatcaat
     6241 ttgctcttgt gctctttgag ccaaagtcgc aacttttcca acttctctat tagatattac
     6301 tttaataagg tttgggtcta tatattgaag agtgtcttct tcaatagaaa tatttaaata
     6361 atcagttgat ttagcatcat gattgaattt ggtacatact agtgtcactc tatatcatcc
     6421 ttctgtaagt tttttgttta cttccaatat agaatgatgt attattgtaa ttgtcgattt
     6481 acaataagtt gttattctag gtggaagttt attgcttttg atgtatagat agacttaacc
     6541 ttttcattat agcatactat agaagaaaat gcaagcaaaa gcatataaac aacaaatgtt
     6601 gtttatatgc ttttgctttt ttgttaattg gagaggacaa ggtgaatatt aacagtcaaa
     6661 atccagttat gtcggttaat gaatcgatga aaattttcaa agctcaagta atgcttagaa
     6721 atttaaagct attaagcgag tggattgtta aaggggactg aagttaaaga cactttttaa
     6781 gaagattttt caattaaagg gtatattaaa actttaattt agtactttat atttttcttg
     6841 actctttaat aatcctatat acagtagaac gactcactcc tgtttgttca gcaatttctt
     6901 caccagacag cttttgttca tcatataaga atttgatctc tcttttctta tgatctggta
     6961 gtgaaggtct tccacctttt ctccctcttg ctcttgcagc ttccaaacct tttttagttc
     7021 gttcacttaa taaattagct tcaagttcag cgaatgcact catcatggta aagaacatct
     7081 tgcccatcgc atctttcgtt gacacattca tatctataat atgtaaatca atgctatttt
     7141 catccaacca ttgagatagc tcaattaatt gttttgttgt acgtccaaga cgatctaatt
     7201 tatagacaac tagaatatct ccttcacgta aataatctaa acacttatca agttccgttc
     7261 ttttagtttt acgaccactc gctttctcgc taaagatacg ttcacaacca tattcctcaa
     7321 gtgtatcaat ctgtccatca agactttgat cttgtgttga cacacgtgca taaccaattt
     7381 tagccatttg aacaccacct tttttaatcg ttttatgggt acttactaat gaaatatagt
     7441 tctcgatgta aataaacacg tctgtcagca tctaaaaact catgattttc gatatttatg
     7501 atacattgat tataacacaa gtttatagac ttaaatatcc aagaaaaacc tcttaatatt
     7561 aagttaagag gttttgtgtc aaaaacgaac gtaatttaga aataatattt attggtattt
     7621 caagaatata tatataatta tttactaagt ccatttaaaa aaatttgaga gaacttgttc
     7681 ataagtttaa ttctttcatt aatattttgt tcatgtgtaa aggtaacaat tccattaaca
     7741 gcgtttgctg ctattttact aacagcatta acatcattaa tacaccattc gccatttaaa
     7801 ttaccctcct taaaaattac atgataagca tctatatatt tgttttctaa tttattcatt
     7861 ttttcattaa tgctattagt tttataatat tcagtgtaaa attcaattat tgcgttttga
     7921 agtggataat agtattcggt tgttaaagat agttcattat ataaataaaa tttttctcta
     7981 ttagttttac atttgatttg ttcctttttc cactgttctt gccatttaga ttcttctata
     8041 tttaaaattt ctaaaaatag attttctttt gttttaaagt gataataaag attcccttta
     8101 ctactttctg ataatttaac aatttctcca gtagtagtgg cattatatcc attttttata
     8161 aataattcct ttgcgacacc tagtatttta tctttcaagt tcatcacgcc tttaatgagt
     8221 attaagtact atcaatgata gcacaaaaaa ttcaaagaat aattgtataa cacaatacag
     8281 ccatttaaat ttcgcaagat tttttgttgt aatatgtaaa aaaaatagat tataatcctt
     8341 atagaccgat cgcacggtct ataaggattg gagggaactt aaatgatttc attttttaca
     8401 aaaactactg atatgatgac atcaaaaaaa agatggactg cactagtagt attagctgtt
     8461 agtttgtttg ttgttacaat ggatatgaca atattaatta tggctttacc ggaattagta
     8521 agagagttag agccttctgg tacccaacag ttatggatag ttgatatata ctctcttgtt
     8581 ttagctggct ttataattcc attgagtgcc tttgctgata aatggggaag aaaaaaagca
     8641 ttattaactg gatttgcttt atttggcctc gtttcattag ctatattttt cgcagaaagt
     8701 gcagagttcg taatagctat tcgattttta cttggtattg caggtgcttt aataatgcca
     8761 actactcttt caatgataag agtaattttt gaaaacccta aagaaagggc cactgcatta
     8821 gctgtatggt caatcgcttc atcgataggt gctgtttttg gaccaattat cggaggagct
     8881 ttacttgagc aattttcatg gcactcggca tttttaatta atgtaccgtt tgcgataata
     8941 gcagttgtag caggtttatt tttattacca gagtctaagt tatcaaaaga aaagtctcac
     9001 tcgtgggata ttccttctac aattttatca attgcaggca tgattggact ggtatggagt
     9061 atcaaagaat tttcaaaaga aggactagca gatattattc catgggttgt aatagtatta
     9121 gcaattacca tgatagtgat atttgttaaa cgtaatttat caagttctga tccaatgtta
     9181 gacgtaagac tttttaaaaa gagatcattt tcagctggta caattgctgc atttatgaca
     9241 atttttgcaa tggcatctgt tttgttatta gcttcacaat ggttacaggt tgtggaagaa
     9301 ctttctcctt ttaaagctgg cttataccta ttacctatgg caataggaga tatggtgttt
     9361 gcaccaattg cacccggatt agcggcgcga tttggaccga aaatagtgtt accttccgga
     9421 attggaattg cagccattgg catgtttatt atgtatttct ttggtcatcc attatcatat
     9481 tctacaatgg ctttagcatt aattttagtt ggagctggta tggcttcact agcagttgca
     9541 tctgctctaa taatgttaga aacacctaca tcaaaagcag gtaatgcagc tgctgttgaa
     9601 gagtctatgt atgaccttgg aaatgttttt ggtgtagcag tacttggtag cctatcttct
     9661 atgctttatc gtgtattttt agatatttca tctttttcat caaaaggtat agttggagat
     9721 ttagctcatg tagctgaaga atctgtagtg ggcgctgtcg aagtagctaa agctacgggg
     9781 ataaaacagc ttgcaaacga ggctgtaaca tcatttaatg atgcttttgt agcaactgct
     9841 ttagtaggtg ggattatcat gattatcatt tcaatagttg tctatttgtt aattcccaaa
     9901 tcacttgata taactaaaca aaaatagtac taaacatata tttttagata ttgtagatta
     9961 gtttattttt aaaattagta atagttaaaa agtagaacca ttaattttaa atggttctac
    10021 ttttttatta ttaagtaaaa ttataaaaga caatttctta aattaataat atgatatacc
    10081 tatatctaat aatttattag tattaataaa ccttgaaagg atgattaact gatgaataga
    10141 gcagatattt ttaaaatatt tgatgtagaa cttaatataa ctatattttt aggagcacta
    10201 gtagcaactt tcatctattt tataagttac atgaacaatg taaatgaatt aattacatta
    10261 tgcttaacat tagcttttcc tgcatattta ttaaaaatta gcagatcgtt cgaggttatt
    10321 ttattagagg aaaaaataaa aaatagtaaa atcaaaatct gtgacaaaga tagaattgaa
    10381 cgagagatac tggtgaaaaa gaacctaata gatgaactat tgtttcctaa tagagttatt
    10441 aaaaaatata cactgacaat tttaatttac agtataggta ttattattct tttactactt
    10501 tgtaatatgt taagttatgg aattggtgaa aatgtcaatt ttatctggag attactagct
    10561 tgttatgtaa cgagtgtcac agtgattata gttgccttga ttataatgat aatgatattt
    10621 gatcttataa ttggattact attaaatttt caaatatttt taaaaaataa aaagtatgat
    10681 gattttttat aattattcga tttatataat gaaaaattaa atgattaaac atagaaacct
    10741 caaaacaaac catatttaat agagtttatg gcgccttata caaataaagg ctatgctgtt
    10801 aaagaattat gtagctatct tcagattaat atggatgatg tttatactat tggcgataga
    10861 aaattgattt acctatgttt aataccctag ataattcttc tgataattta gtttttgtta
    10921 gagtttaaca taactgatga ttatcagaag tttgattcac attaaaaagg cacaacaagt
    10981 tcatgaactt gtgtgccttt ttaattgcaa ttagttacca aataaacgac tccaaaatct
    11041 ctttttaggt ggttctacct ccctattatc actggaatcg ttcttcgttt tttcaacaaa
    11101 cttatcctct tttgttaatt catctgtact gtcatgctta ttctcttcag ttatattttt
    11161 atgatttact tcggttggtt gacattcttt ttgttgttgg ttgatatttt gagattcttc
    11221 agtaaaagtt gcttcttgtg gattaatatt ctgtctatta ttcatcgttg tatcaaaaga
    11281 atatttaagc tgtctttctt cttctaattg gctttctagt ttttgaattt ttttattgtt
    11341 ttctaaagat aatatttgtt gattctccaa cagtttattt aatgtattaa cattgctatt
    11401 ttgttgatct aattgtttag ttagattctc aatatatttt tcatcatttt tagctctagt
    11461 ttcaaagatt tctatttgct tttctaattc gtttactttt gtttttaatg tctcagaact
    11521 atttgaatta ttatgtatat acttttcaac ttttgcagta ttttcattaa ataattcatt
    11581 ttttactgat tctttattat gagttgtttt ctctttctta gtgatttttt ctataatttt
    11641 taaataatca ttatctttaa tataattaat tccattttct ttttcaaaag atatatttaa
    11701 tcttttagca ttattaacaa tagtttgttt agttacacct aattcatcag caatcatttt
    11761 aatagttttc ataacatccc cccttaggta cctaatttag tacctaaatt ttaccaccta
    11821 acaaaattta tttcatcatt tagtactaaa tttactatct attaactacc taaatgccta
    11881 attatctatc cttaaaatat tttcgccact taattaggtg ctaaaatagc gaattatacg
    11941 tttggtagtt ttaggtgtac ttttaattac atttaaaact ctttatatac gccattaaaa
    12001 gtgttaatat tactttataa atattaaaag agtcgatgct attggcgtag catcgactct
    12061 cggtaataaa acgattcgca ctcgtttgtt tatatatttt tttgatactt gtattatata
    12121 tatctaatca tctaagtgca agcacaaaac atataactta cgtaaaaatt gttttattac
    12181 ctcaatccca aaatggaaat gaggttttta ttatgcccaa ttttgaaaaa tataatttat
    12241 cacaagtaaa aactgaaaga ttttatcaac tgcctaaata tttatttgaa gatgcatatt
    12301 ttaagaaaat gtctgcagaa gccaaaatta tgtatgcgtt attaaaagat cgttttgaat
    12361 tatccctcca aaatgaatgg gtagataaaa ataataatat ttactttatt ttcagtaata
    12421 aacatttgtg tgaatactta ggttatgcag aacaaaaaat tataaaatta aaaaaagagt
    12481 taataaaatt taatttacta actcaagaac gtgttggcct taataaacca aatagattat
    12541 acctattaaa acctaattat gacattgaag ccagtcatat caaggaactt ccaaattcac
    12601 agttccagaa caatgaattt ggaagttcta gaactgtgaa tttaagtggt caagaacttc
    12661 caaattcaca gtctaatgat actgattata atgacactga ttatattaag actaattata
    12721 atgatatgta tgatttgaat gataggaaat taacatattc tagtaatcat acaaatcatt
    12781 caaatcacta ctattcaaaa ttcaatgatg aagctttaaa atttcaatta cttgaagaac
    12841 taccacaaag tattcaaagt tatttaagta acttttctgt atctgaaatt aaacttatta
    12901 aatctgtgtt attaaaagcc aaaacatcct tcaacaattc tattgatgca tattatttat
    12961 tagaagatat ggaatttgaa attgttaatg ttcttaagcg ttttaaagct acattaattc
    13021 aaaaaaatga aaccgttgaa gcaatgcaag ggtacttaat gaaatctctt aaatctgaat
    13081 ttgcagaagt acatacgctt aataaacgac gtgatcattt accaatcact tctttattta
    13141 atcaataata caacttaata agccaacaat gatccaatta gagaatatct ttaattagat
    13201 gttctcttaa ctgtaagcga actttatttt caatgaaatg taattaatta agtaagtggg
    13261 actaaaactt ttctttttta gtcccatttt tataaacaat tattattcat tgttactact
    13321 atcagcaatt ttttcttttt agtgaaaccc aagtatttgt aagcatgata aattacttct
    13381 ttttatattt attgggttta tttgtattaa tgtagttgga atacatcttt aacaattctt
    13441 ctgtttcatc aggacttaaa tgctcacatt caaaatactt ttcaagcatt gcaccttttc
    13501 gaatcagtct atgtgctctt tcttttctat ctaaattttt taatctatgc tttcttaatt
    13561 ctgctttttc
//
