LOCUS       NZ_CP073825            13570 bp    DNA     circular CON 16-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1200599 plasmid p1B1200599,
            complete sequence.
ACCESSION   NZ_CP073825
VERSION     NZ_CP073825.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699872
            Assembly: GCF_019329245.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 357x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019329245.1-RS_2024_04_16
            Annotation Date                   :: 04/16/2024 08:02:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,685
            CDSs (total)                      :: 2,604
            Genes (coding)                    :: 2,525
            CDSs (with protein)               :: 2,525
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 44 of 79
            Pseudo Genes (internal stop)      :: 28 of 79
            Pseudo Genes (multiple problems)  :: 24 of 79
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073825.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1200599"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p1B1200599"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(11939..13570,1..291))
                     /locus_tag="KFV28_RS13195"
                     /old_locus_tag="KFV28_13205"
     CDS             complement(join(11939..13570,1..291))
                     /locus_tag="KFV28_RS13195"
                     /old_locus_tag="KFV28_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            500..769
                     /locus_tag="KFV28_RS13200"
                     /old_locus_tag="KFV28_13210"
     CDS             500..769
                     /locus_tag="KFV28_RS13200"
                     /old_locus_tag="KFV28_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            786..1025
                     /locus_tag="KFV28_RS13205"
                     /old_locus_tag="KFV28_13215"
     CDS             786..1025
                     /locus_tag="KFV28_RS13205"
                     /old_locus_tag="KFV28_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(1250..2185)
                     /locus_tag="KFV28_RS13210"
                     /old_locus_tag="KFV28_13220"
     CDS             complement(1250..2185)
                     /locus_tag="KFV28_RS13210"
                     /old_locus_tag="KFV28_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            2626..3387
                     /locus_tag="KFV28_RS13215"
                     /old_locus_tag="KFV28_13225"
     CDS             2626..3387
                     /locus_tag="KFV28_RS13215"
                     /old_locus_tag="KFV28_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            complement(3706..4266)
                     /locus_tag="KFV28_RS13225"
                     /old_locus_tag="KFV28_13235"
     CDS             complement(3706..4266)
                     /locus_tag="KFV28_RS13225"
                     /old_locus_tag="KFV28_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(4471..6015)
                     /gene="qac"
                     /locus_tag="KFV28_RS13230"
                     /old_locus_tag="KFV28_13240"
     CDS             complement(4471..6015)
                     /gene="qac"
                     /locus_tag="KFV28_RS13230"
                     /old_locus_tag="KFV28_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            6194..6760
                     /gene="qacR"
                     /locus_tag="KFV28_RS13235"
                     /old_locus_tag="KFV28_13245"
     ARG             6195..6757
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            7011..7571
                     /locus_tag="KFV28_RS13240"
                     /old_locus_tag="KFV28_13250"
     CDS             7011..7571
                     /locus_tag="KFV28_RS13240"
                     /old_locus_tag="KFV28_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            7990..8901
                     /locus_tag="KFV28_RS13245"
                     /old_locus_tag="KFV28_13255"
     CDS             7990..8901
                     /locus_tag="KFV28_RS13245"
                     /old_locus_tag="KFV28_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(9078..9176)
                     /locus_tag="KFV28_RS13250"
                     /old_locus_tag="KFV28_13260"
     CDS             complement(9078..9176)
                     /locus_tag="KFV28_RS13250"
                     /old_locus_tag="KFV28_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            9340..10038
                     /locus_tag="KFV28_RS13255"
                     /old_locus_tag="KFV28_13265"
     CDS             9340..10038
                     /locus_tag="KFV28_RS13255"
                     /old_locus_tag="KFV28_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            10189..10893
                     /locus_tag="KFV28_RS13260"
                     /old_locus_tag="KFV28_13270"
     CDS             10189..10893
                     /locus_tag="KFV28_RS13260"
                     /old_locus_tag="KFV28_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(10942..11175)
                     /locus_tag="KFV28_RS13265"
                     /old_locus_tag="KFV28_13275"
     CDS             complement(10942..11175)
                     /locus_tag="KFV28_RS13265"
                     /old_locus_tag="KFV28_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(11252..11665)
                     /locus_tag="KFV28_RS13560"
                     /old_locus_tag="KFV28_13280"
     CDS             complement(11252..11665)
                     /locus_tag="KFV28_RS13560"
                     /old_locus_tag="KFV28_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     ARG             complement(4475..6015)
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          complement(1251..2185)
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2..291)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.66e-55"
     Typing          complement(12009..13570)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 caaacttaat aagacttctt ttgctaatct aggatttttt gttttcgcat tattcttttc
       61 aactttatca acttcattcc ataaacgctg tctatcttta gtccattcgg gtgcatgttg
      121 aggagttaaa atcatgcttt caggcttaat agtatgattt ctaaacttaa ttttttcatc
      181 tgtacgttcg ctatataatg cttcatcaga acgataagac gctgacgcaa cgatattttt
      241 tgttgctcga ttaatgttag tcactctcaa cataaagtaa cctttattca tgattttacc
      301 tcctctcttt tctgtttttt tacccctgtg ggaactataa tgatcaacca gtatggttgc
      361 ccatttttat gccaattttc aatttgcatg taaatgggca gtgcagtgtc ttaagaactg
      421 gttatattat accatggatg tgcgacttac ccaatgaatt atggtatgct ttatatagat
      481 aaactaaggg agttttttaa tgtctaaaat taaacaaaca gatattacaa aattaagtca
      541 attagaaaaa caaattgcac aactgaaaga gcaaaagaaa aaacaagaag aacaactatc
      601 taataaaatt ggtggttatt ttttaaataa acttgattta aatatgatca actctagtga
      661 tgaattgtat aaaattatgg ataaagtcat tggagaattt aataaagata tcgaacaaaa
      721 tagcagcaac gagaacaagg aaaatttaac agaagacaaa attaactaga atagtaagtg
      781 atacgatgaa tgattttaaa catctcaaaa aaataaatgc tgaaattgaa aaagcagaat
      841 taagaaagca tagattaaaa aatttagata gaaaagaaag agcacataga ctgattcgaa
      901 aaggtgcaat gcttgaaaag tattttgaat gtgagcattt aagtcctgat gaaacagaag
      961 aattgttaaa gatgtattcc aactacatta atacaaataa acccaataaa tataaaaaga
     1021 agtaatttat catgcttaca aatacttggg tttcactaaa aagaaaaaat tgctgatagt
     1081 agtaacaatg aataataatt gtttataaaa atgggactaa aaaagaaaag ttttagtccc
     1141 acttacttaa ttaattacat ttcattgaaa ataaagttcg cttacagtta agagaacatc
     1201 taattaaaga tattctctaa ttggatcatt gttggcttat taagttgtat tattgattaa
     1261 ataaagaagt gattggtaaa tgatcacgtc gtttattaag cgtatgtact tctgcaaatt
     1321 cagatttaag agatttcatt aagtaccctt gcattgcttc aacggtttca tttttttgaa
     1381 ttaatgtagc tttaaaacgc ttaagaacat taacaatttc aaattccata tcttctaata
     1441 aataatatgc atcaatagaa ttgttgaagg atgttttggc ttttaataac acagatttaa
     1501 taagtttaat ttcagataca gaaaagttac ttaaataact ttgaatactt tgtggtagtt
     1561 cttcaagtaa ttgaaatttt aaagcttcat cattgaattt tgaatagtag tgatttgaat
     1621 gatttgtatg attactagaa tatgttaatt tcctatcatt caaatcatac atatcattat
     1681 aattagtctt aatataatca gtgtcattat aatcagtatc attagactgt gaatttggaa
     1741 gttcttgacc acttaaattc acagttctag aacttccaaa ttcattgttc tggaactgtg
     1801 aatttggaag ttccttgata tgactggctt caatgtcata attaggtttt aataggtata
     1861 atctatttgg tttattaagg ccaacacgtt cttgagttag taaattaaat tttattaact
     1921 ctttttttaa ttttataatt ttttgttctg cataacctaa gtattcacac aaatgtttat
     1981 tactgaaaat aaagtaaata ttattatttt tatctaccca ttcattttgg agggataatt
     2041 caaaacgatc ttttaataac gcatacataa ttttggcttc tgcagacatt ttcttaaaat
     2101 atgcatcttc aaataaatat ttaggcagtt gataaaatct ttcagttttt acttgtgata
     2161 aattatattt ttcaaaattg ggcataataa aaacctcatt tccattttgg gattgaggta
     2221 ataaaacaat ttttacgtaa gttatatgtt ttgtgcttgc acttagatga ttagatatat
     2281 ataatacaag tatcaaaaaa atatataaac aaacgagtgc gaatcgtttt attaccgaga
     2341 gtcgatgcta cgccaatagc atcgactctt ttaatattta taaagtaata ttaacacttt
     2401 taatggcgta tataaagagt tttaaatgta attaaaagta cacctaaaac taccaaacgt
     2461 ataattcgct attttagcac ctaattaagt ggcgaaaata ttttaaggat agataattag
     2521 gcatttaggt agttaataga tagtaaattt agtactaaat gatgaaataa attttgttag
     2581 gtggtaaaat ttaggtacta aattaggtac ctaagggggg atgttatgaa aactattaaa
     2641 atgattgctg atgaattagg tgtaactaaa caaactattg ttaataatgc taaaagatta
     2701 aatatatctt ttgaaaaaga aaatggaatt aattatatta aagataatga ttatttaaaa
     2761 attatagaaa aaatcactaa gaaagagaaa acaactcata ataaagaatc agtaaaaaat
     2821 gaattattta atgaaaatac tgcaaaagtt gaaaagtata tacataataa ttcaaatagt
     2881 tctgagacat taaaaacaaa agtaaacgaa ttagaaaagc aaatagaaat ctttgaaact
     2941 agagctaaaa atgatgaaaa atatattgag aatctaacta aacaattaga tcaacaaaat
     3001 agcaatgtta atacattaaa taaactgttg gagaatcaac aaatattatc tttagaaaac
     3061 aataaaaaaa ttcaaaaact agaaagccaa ttagaagaag aaagacagct taaatattct
     3121 tttgatacaa cgatgaataa tagacagaat attaatccac aagaagcaac ttttactgaa
     3181 gaatctcaaa atatcaacca acaacaaaaa gaatgtcaac caaccgaagt aaatcataaa
     3241 aatataactg aagagaataa gcatgacagt acagatgaat taacaaaaga ggataagttt
     3301 gttgaaaaaa cgaagaacga ttccagtgat aatagggagg tagaaccacc taaaaagaga
     3361 ttttggagtc gtttatttgg taactaattg caattaaaaa ggcacacaag ttcatgaact
     3421 tgttgtgcct ttttaatgtg aatcaaactt ctgataatca tcagttatgt taaactctaa
     3481 caaaaactaa attatcagaa gaattatcta gggtattaaa cataggtaaa tcaattttct
     3541 atcgccaata gtataaacat catccatatt aatctgaaga tagctacata attctttaac
     3601 agcatagcct ttatttgtat aaggcgccat aaactctatt aaatatggtt tgttttgagg
     3661 tttctatgtt taatcattta atttttcatt atataaatcg aataattata aaaaatcatc
     3721 atacttttta ttttttaaaa atatttgaaa atttaatagt aatccaatta taagatcaaa
     3781 tatcattatc attataatca aggcaactat aatcactgtg acactcgtta cataacaagc
     3841 tagtaatctc cagataaaat tgacattttc accaattcca taacttaaca tattacaaag
     3901 tagtaaaaga ataataatac ctatactgta aattaaaatt gtcagtgtat attttttaat
     3961 aactctatta ggaaacaata gttcatctat taggttcttt ttcaccagta tctctcgttc
     4021 aattctatct ttgtcacaga ttttgatttt actatttttt attttttcct ctaataaaat
     4081 aacctcgaac gatctgctaa tttttaataa atatgcagga aaagctaatg ttaagcataa
     4141 tgtaattaat tcatttacat tgttcatgta acttataaaa tagatgaaag ttgctactag
     4201 tgctcctaaa aatatagtta tattaagttc tacatcaaat attttaaaaa tatctgctct
     4261 attcatcagt taatcatcct ttcaaggttt attaatacta ataaattatt agatataggt
     4321 atatcatatt attaatttaa gaaattgtct tttataattt tacttaataa taaaaaagta
     4381 gaaccattta aaattaatgg ttctactttt taactattac taattttaaa aataaactaa
     4441 tctacaatat ctaaaaatat atgtttagta ctatttttgt ttagttatat caagtgattt
     4501 gggaattaac aaatagacaa ctattgaaat gataatcatg ataatcccac ctactaaagc
     4561 agttgctaca aaagcatcat taaatgatgt tacagcctcg tttgcaagct gttttatccc
     4621 cgtagcttta gctacttcga cagcgcccac tacagattct tcagctacat gagctaaatc
     4681 tccaactata ccttttgatg aaaaagatga aatatctaaa aatacacgat aaagcataga
     4741 agataggcta ccaagtactg ctacaccaaa aacatttcca aggtcataca tagactcttc
     4801 aacagcagct gcattacctg cttttgatgt aggtgtttct aacattatta gagcagatgc
     4861 aactgctagt gaagccatac cagctccaac taaaattaat gctaaagcca ttgtagaata
     4921 tgataatgga tgaccaaaga aatacataat aaacatgcca atggctgcaa ttccaattcc
     4981 ggaaggtaac actattttcg gtccaaatcg cgccgctaat ccgggtgcaa ttggtgcaaa
     5041 caccatatct cctattgcca taggtaatag gtataagcca gctttaaaag gagaaagttc
     5101 ttccacaacc tgtaaccatt gtgaagctaa taacaaaaca gatgccattg caaaaattgt
     5161 cataaatgca gcaattgtac cagctgaaaa tgatctcttt ttaaaaagtc ttacgtctaa
     5221 cattggatca gaacttgata aattacgttt aacaaatatc actatcatgg taattgctaa
     5281 tactattaca acccatggaa taatatctgc tagtccttct tttgaaaatt ctttgatact
     5341 ccataccagt ccaatcatgc ctgcaattga taaaattgta gaaggaatat cccacgagtg
     5401 agacttttct tttgataact tagactctgg taataaaaat aaacctgcta caactgctat
     5461 tatcgcaaac ggtacattaa ttaaaaatgc cgagtgccat gaaaattgct caagtaaagc
     5521 tcctccgata attggtccaa aaacagcacc tatcgatgaa gcgattgacc atacagctaa
     5581 tgcagtggcc ctttctttag ggttttcaaa aattactctt atcattgaaa gagtagttgg
     5641 cattattaaa gcacctgcaa taccaagtaa aaatcgaata gctattacga actctgcact
     5701 ttctgcgaaa aatatagcta atgaaacgag gccaaataaa gcaaatccag ttaataatgc
     5761 tttttttctt ccccatttat cagcaaaggc actcaatgga attataaagc cagctaaaac
     5821 aagagagtat atatcaacta tccataactg ttgggtacca gaaggctcta actctcttac
     5881 taattccggt aaagccataa ttaatattgt catatccatt gtaacaacaa acaaactaac
     5941 agctaatact actagtgcag tccatctttt ttttgatgtc atcatatcag tagtttttgt
     6001 aaaaaatgaa atcatttaag ttccctccaa tccttataga ccgtgcgatc ggtctataag
     6061 gattataatc tatttttttt acatattaca acaaaaaatc ttgcgaaatt taaatggctg
     6121 tattgtgtta tacaattatt ctttgaattt tttgtgctat cattgatagt acttaatact
     6181 cattaaaggc gtgatgaact tgaaagataa aatactaggt gtcgcaaagg aattatttat
     6241 aaaaaatgga tataatgcca ctactactgg agaaattgtt aaattatcag aaagtagtaa
     6301 agggaatctt tattatcact ttaaaacaaa agaaaatcta tttttagaaa ttttaaatat
     6361 agaagaatct aaatggcaag aacagtggaa aaaggaacaa atcaaatgta aaactaatag
     6421 agaaaaattt tatttatata atgaactatc tttaacaacc gaatactatt atccacttca
     6481 aaacgcaata attgaatttt acactgaata ttataaaact aatagcatta atgaaaaaat
     6541 gaataaatta gaaaacaaat atatagatgc ttatcatgta atttttaagg agggtaattt
     6601 aaatggcgaa tggtgtatta atgatgttaa tgctgttagt aaaatagcag caaacgctgt
     6661 taatggaatt gttaccttta cacatgaaca aaatattaat gaaagaatta aacttatgaa
     6721 caagttctct caaatttttt taaatggact tagtaaataa ttatatatat attcttgaaa
     6781 taccaataaa tattatttct aaattacgtt cgtttttgac acaaaacctc ttaacttaat
     6841 attaagaggt ttttcttgga tatttaagtc tataaacttg tgttataatc aatgtatcat
     6901 aaatatcgaa aatcatgagt ttttagatgc tgacagacgt gtttatttac atcgagaact
     6961 atatttcatt agtaagtacc cataaaacga ttaaaaaagg tggtgttcaa atggctaaaa
     7021 ttggttatgc acgtgtgtca acacaagatc aaagtcttga tggacagatt gatacacttg
     7081 aggaatatgg ttgtgaacgt atctttagcg agaaagcgag tggtcgtaaa actaaaagaa
     7141 cggaacttga taagtgttta gattatttac gtgaaggaga tattctagtt gtctataaat
     7201 tagatcgtct tggacgtaca acaaaacaat taattgagct atctcaatgg ttggatgaaa
     7261 atagcattga tttacatatt atagatatga atgtgtcaac gaaagatgcg atgggcaaga
     7321 tgttctttac catgatgagt gcattcgctg aacttgaagc taatttatta agtgaacgaa
     7381 ctaaaaaagg tttggaagct gcaagagcaa gagggagaaa aggtggaaga ccttcactac
     7441 cagatcataa gaaaagagag atcaaattct tatatgatga acaaaagctg tctggtgaag
     7501 aaattgctga acaaacagga gtgagtcgtt ctactgtata taggattatt aaagagtcaa
     7561 gaaaaatata aagtactaaa ttaaagtttt aatataccct ttaattgaaa aatcttctta
     7621 aaaagtgtct ttaacttcag tcccctttaa caatccactc gcttaatagc tttaaatttc
     7681 taagcattac ttgagctttg aaaattttca tcgattcatt aaccgacata actggatttt
     7741 gactgttaat attcaccttg tcctctccaa ttaacaaaaa agcaaaagca tataaacaac
     7801 atttgttgtt tatatgcttt tgcttgcatt ttcttctata gtatgctata atgaaaaggt
     7861 taagtctatc tatacatcaa aagcaataaa cttccaccta gaataacaac ttattgtaaa
     7921 tcgacaatta caataataca tcattctata ttggaagtaa acaaaaaact tacagaagga
     7981 tgatatagag tgacactagt atgtaccaaa ttcaatcatg atgctaaatc aactgattat
     8041 ttaaatattt ctattgaaga agacactctt caatatatag acccaaacct tattaaagta
     8101 atatctaata gagaagttgg aaaagttgcg actttggctc aaagagcaca agagcaaatt
     8161 gatgtttttt ttgaaaaaat tatcgatata catcttagtg atttttctga tacccaaaag
     8221 cgtaatgaat ttagaaaatt attctctcat ttttcagaac cacaccattt acgcttaggt
     8281 ttttctcaga aaggtaatac aggaaaaggt actacagcat ctgaattgat taatctattt
     8341 atgaatgaag atattctttc cattatctta aatgatgacg gattaacaat acctcaaaaa
     8401 gcaccattaa tcagaaattt tggtgatgat aaattatcag atttaacaag taatataatc
     8461 atgaatgtta taattgattt taataaatcc atgattcagt catttccaga aatgaaatcg
     8521 tttctttcta aagaaactgt gacatattat tatttctcta ctaacggtat atgggaaact
     8581 tatgatttca ctccatttct ttttgatggt aataaaactt tattagttcc aaaattattc
     8641 actacttata atcagacatc tagtttagat ttgatgataa gagtatacat tgaagaagaa
     8701 atcattagat tgcaaaaaca acgtaataca accaaaaaaa tgacaaaaaa acaatttatc
     8761 aatgaattta taaaaggaca tcgtatggat aatatgacct atttcttttt aaacacttct
     8821 tcaaaatcac acaaaaaatt tcaaaaagaa ttagattcaa aagccaatga aaggggtaac
     8881 aataacaaag atgataatta gtacttacta atcagtatag tatagactta acatactaat
     8941 tattcaattt tgcattcctc cacactatga tgttataatt tagataacaa atatgtgttg
     9001 aacatggtaa acaccgagcc cgataactat tccagtagtt atcgggctct ttttggtgtt
     9061 attactgtgt cgtcgcttta ttgtttatca cgattattca accaatgtga aaacaacgtg
     9121 attataaccc ctactataat aggagctact atagatacaa atatgatgct gaacatggtg
     9181 tcacctcctt ccatgctaaa ccagcacaga agtagaacga cgacttttat attatatcat
     9241 ttgtgaattg ttaagcatat gcacttccga taagtcattt tatgtttaat gtatctatgt
     9301 gataatgaaa atacaaattc tattaaagga gataatttta tgaaaaaacg atcattactt
     9361 ccaactttat tattggctag ctcattaaca ttcacaggtt tttcagcgac agccaatgct
     9421 gaggatggtg ccaaaaccgt atacggagag aacttaccaa atagcgcaga tacaaatgga
     9481 gatggttggg ctaatgtaga cttcaatact tcatccctcc cacaatctgc acaaaatcaa
     9541 ataagtgaac tttctaaaca gaaagattca ggggaattaa ctcaaatcga gtataatgag
     9601 aaagtttcaa gtgtatttaa tgaatatcaa tcacaaagta ctgatataca aaataatgag
     9661 agaactcaac aaggtaaaag acctttcgga ggggtgatac ctaattgtat gactcaagaa
     9721 gaataccaag aattagaaag tcatgttcct aaccctaata gtgtatccac agaaaaatat
     9781 aatcaaattg taaaaaatga aagtcaaaag atagctaatg aagaaaataa aactattaat
     9841 acacctcaag gtgtgttgac acctgtaact gaaaataaaa acaagagttc tgaaggaatg
     9901 aaaaatcaag attcaaactc tcaagtatta ccagaaacag gagaaagcaa ccaaagtgat
     9961 atattgtcaa taattggcgc ttctggacta ctaatattag gtataagtct tttaatattc
    10021 agaaaaaagg caatataaaa attgataaaa taaccaccgt gtttatgtaa aacatgtggt
    10081 tattttatta tatatttaat acatattttt caactcatgt aatttacgtt ttcttgctga
    10141 aaaaatatta acataatcta atttgaaata ctgaaaagga gttgtaatat gaaaaaaatc
    10201 attttaccta tattaattgt tagtttatcg gtaattttag tgagttgtaa taataacgat
    10261 tcagaacatc acaatgataa taattctagt gatactaaca cctcagaaaa caaagataat
    10321 gaagcatcaa atgaaattga cgaaaaaact aacacatctg atagtgcgga taattcagaa
    10381 agtagtaata aacaaaataa agaaaaaaat gataataaaa aacaagaaaa aaataatgat
    10441 agtaataaga agcaacaatc tactgataaa aataaaagtt acttaaataa ttttaactcg
    10501 aaagaaattg aatatgcacg agtttggtat cagttaatat ctacaagaaa tgatttaaaa
    10561 ggtattaaaa atgtttatgt tacagaaata ccaaaaggct caaaagtaaa tccacaagcc
    10621 aaaaatagtg caatttataa agaaaaagtt gtgaaattag aagctcctat gagagctggt
    10681 gggtctgtaa catatagcag taatggtgat ggaacaatta atgtatataa taatatccct
    10741 tataaatggg aaaattcaca aaatagtgat tatagtcaaa tgaataagat aacaaaaaaa
    10801 gcaatagagg agaatattga aacagtctat attaaaccct acgacaataa aacagtagct
    10861 aagttagcaa gtaaaattag atataataag tagttagtag attattaaaa aggcgcctaa
    10921 aattaggcgt cttttttatc attagttcaa aagataagca taacgttcca tgtaacagca
    10981 tataaagtaa tgaatcaatt catcactatc taatgccacc attgttagtt cagccttgca
    11041 tggtttaacg tatctcacat aatggtacaa atcttcaaat agtgcctgtt tttcgggcat
    11101 tttaaacacc ttgtgtacat taaaacctat ttcttctaat cgctttgtaa tcacgccgaa
    11161 aacgaatgtg ttcatgtgaa atcactccat catttttaat attcacatgt acactaaaaa
    11221 aaatccgata catcggcttt ttatttttgg tttaatttat ttctatattg ttttcactat
    11281 cgctcggacg cataagtgtt aaaacacctt ctaccatacc tatcacaccc ggtattcccg
    11341 tccaacaaaa tagtaaataa agaagagcat ttttattttt cttaacataa aacttatgag
    11401 ccccgaaacc tcctagaaaa aatgctaatg cagagtaaac atatttatta acttgcatga
    11461 tttccactcc tttaattaat tgatttagag aaacattaaa taatttagtg atttctaaaa
    11521 ttgtttttaa gtcgggatta cttacttctc tttcccaatt agaaatagaa tgtctcgata
    11581 cccctaactt ttcagctaat ttttcttgag acatgttttt actttttctt aacttttgta
    11641 tttgatgtcc aatttttgtt tccaaattat ttttctcctt aactttaaga tatatttaaa
    11701 ctcaattttt tctatcgata tttatgaaca ttagtatttt ttatgctgaa atatgtgaac
    11761 aatcgcataa tatcaaggtt tttctataat tatacacgtt ttaaaataca ttgtgataat
    11821 acatagaatg taccttatct aagtatataa atgttttctt taaaccaaag ctaatttacc
    11881 acattgggtt aagtttctta ttattaaaga atgaactttg tatgatttca actgcttact
    11941 atcttctcat ttggttattt ctctcttgtt tcttcttctt tttctgatga ttaatattgt
    12001 tttgcttttc agtttcgttc gcatagtaca taagaaagtc actagcattt atcgttggta
    12061 aattaatgtg attagtttgt tcattttcat gttcaatacg attgtcatga tcatttctat
    12121 ctacttgatt aatatgtttt tcgataactt cttcttgatc gacatgatta tatttctcaa
    12181 gtttaatgag taattgaact ttttcattag gtgtttggtc tttgactttt tcaaagtttt
    12241 tatcgcctat atctattctt aaatcatcat caatatgttc tttataacta tcaatttctt
    12301 tttctttttc cttaataaat tgcaactcaa cgttatattc aaagctatct ttttctaaat
    12361 ttttaagatg tttttgacta aagaaaatat ctttactcgc ttttttataa gtatcttcta
    12421 gcaatacatc attaggaata ttgttatttt gtttgaacag gtattgaaca tgagttgctt
    12481 ttataatatt acctttttgt gcctcatcaa caataaatgc tttttcatca tggctaaaat
    12541 gatgatgaga tttagggtac atttgctgaa caatgagatg atttttttca ctttcagtta
    12601 gataaacatc tttagctgat tgataaatct ctttacgttt ttcctctcta ctttctaatt
    12661 tattcttctc attgagtaac gctttatagt tagtttcaac agtattaaca aactcttgtt
    12721 gactatatcc tttactttca atttcttttc taaactgata attttcatca taatttttag
    12781 catagaattt taattgacgt ctcttaattt catttttaag taaatcattt tgaattttac
    12841 gaccaaaggt agacgcactg tcaaacgtat ttttaattgt ttctttcgcc tctgaatagc
    12901 caacatcacg ttgatatcta tcaaataaaa tagaataact ttgttcattt ttacttactt
    12961 gttcgttagc attttgaata aataatctaa tttcattttg tgcttgttct ttctctttat
    13021 cactaaataa ttgtgatact tcgccacgat tatatcttct aatttgttca tttttctgtc
    13081 cgtaataagt aacgggttgg tactcgatac cctctttttc acagcgtttt ctttcatttt
    13141 gttctaatct atattcttct cttgtaagtg gaatttcggc aattttttct aaaccttgcg
    13201 cttcaaaaga acgactatca tatcgtctta aactttgttc acgttctgag tattgattta
    13261 attttaattc ccaatgctta cgaacttctt tgaaatcaaa gtcggcaaat cgttcttgtt
    13321 ttctaacttt attaccattt ttatttaaaa tcgcattgcc attttcatca tattgcgttc
    13381 tcgtttttgt ctttttcccc caagtaccgt ctgcattaaa cggtctttga gtaagtagaa
    13441 catgggcgtg tgggttgttt atatcatctc tatgaattga aatatcagca accattcctt
    13501 tgtctacaaa ctcacttttt ataaaatctt tagttaattc agtttgtacc tctctatcaa
    13561 aatcattagg
//
