LOCUS       NZ_CP073822            13570 bp    DNA     circular CON 17-APR-2024
DEFINITION  Staphylococcus epidermidis strain B1200343 plasmid p1B1200343,
            complete sequence.
ACCESSION   NZ_CP073822
VERSION     NZ_CP073822.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699871
            Assembly: GCF_019329325.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 467x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019329325.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 06:56:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,689
            CDSs (total)                      :: 2,608
            Genes (coding)                    :: 2,525
            CDSs (with protein)               :: 2,525
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 44 of 83
            Pseudo Genes (incomplete)         :: 44 of 83
            Pseudo Genes (internal stop)      :: 28 of 83
            Pseudo Genes (multiple problems)  :: 24 of 83
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073822.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="B1200343"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p1B1200343"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(11904..13570,1..256))
                     /locus_tag="KFV43_RS13210"
                     /old_locus_tag="KFV43_13220"
     CDS             complement(join(11904..13570,1..256))
                     /locus_tag="KFV43_RS13210"
                     /old_locus_tag="KFV43_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            465..734
                     /locus_tag="KFV43_RS13215"
                     /old_locus_tag="KFV43_13225"
     CDS             465..734
                     /locus_tag="KFV43_RS13215"
                     /old_locus_tag="KFV43_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            751..990
                     /locus_tag="KFV43_RS13220"
                     /old_locus_tag="KFV43_13230"
     CDS             751..990
                     /locus_tag="KFV43_RS13220"
                     /old_locus_tag="KFV43_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(1215..2150)
                     /locus_tag="KFV43_RS13225"
                     /old_locus_tag="KFV43_13235"
     CDS             complement(1215..2150)
                     /locus_tag="KFV43_RS13225"
                     /old_locus_tag="KFV43_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            2591..3352
                     /locus_tag="KFV43_RS13230"
                     /old_locus_tag="KFV43_13240"
     CDS             2591..3352
                     /locus_tag="KFV43_RS13230"
                     /old_locus_tag="KFV43_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            complement(3671..4231)
                     /locus_tag="KFV43_RS13240"
                     /old_locus_tag="KFV43_13250"
     CDS             complement(3671..4231)
                     /locus_tag="KFV43_RS13240"
                     /old_locus_tag="KFV43_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(4436..5980)
                     /gene="qac"
                     /locus_tag="KFV43_RS13245"
                     /old_locus_tag="KFV43_13255"
     CDS             complement(4436..5980)
                     /gene="qac"
                     /locus_tag="KFV43_RS13245"
                     /old_locus_tag="KFV43_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            6159..6725
                     /gene="qacR"
                     /locus_tag="KFV43_RS13250"
                     /old_locus_tag="KFV43_13260"
     ARG             6160..6722
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6976..7536
                     /locus_tag="KFV43_RS13255"
                     /old_locus_tag="KFV43_13265"
     CDS             6976..7536
                     /locus_tag="KFV43_RS13255"
                     /old_locus_tag="KFV43_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            7955..8866
                     /locus_tag="KFV43_RS13260"
                     /old_locus_tag="KFV43_13270"
     CDS             7955..8866
                     /locus_tag="KFV43_RS13260"
                     /old_locus_tag="KFV43_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(9043..9141)
                     /locus_tag="KFV43_RS13265"
                     /old_locus_tag="KFV43_13275"
     CDS             complement(9043..9141)
                     /locus_tag="KFV43_RS13265"
                     /old_locus_tag="KFV43_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            9305..10003
                     /locus_tag="KFV43_RS13270"
                     /old_locus_tag="KFV43_13280"
     CDS             9305..10003
                     /locus_tag="KFV43_RS13270"
                     /old_locus_tag="KFV43_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            10154..10858
                     /locus_tag="KFV43_RS13275"
                     /old_locus_tag="KFV43_13285"
     CDS             10154..10858
                     /locus_tag="KFV43_RS13275"
                     /old_locus_tag="KFV43_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(10907..11140)
                     /locus_tag="KFV43_RS13280"
                     /old_locus_tag="KFV43_13290"
     CDS             complement(10907..11140)
                     /locus_tag="KFV43_RS13280"
                     /old_locus_tag="KFV43_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(11217..11630)
                     /locus_tag="KFV43_RS13565"
                     /old_locus_tag="KFV43_13295"
     CDS             complement(11217..11630)
                     /locus_tag="KFV43_RS13565"
                     /old_locus_tag="KFV43_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     ARG             complement(4440..5980)
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          complement(1216..2150)
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11974..13568)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 tttttgtttt cgcattattc ttttcaactt tatcaacttc attccataaa cgctgtctat
       61 ctttagtcca ttcgggtgca tgttgaggag ttaaaatcat gctttcaggc ttaatagtat
      121 gatttctaaa cttaattttt tcatctgtac gttcgctata taatgcttca tcagaacgat
      181 aagacgctga cgcaacgata ttttttgttg ctcgattaat gttagtcact ctcaacataa
      241 agtaaccttt attcatgatt ttacctcctc tcttttctgt ttttttaccc ctgtgggaac
      301 tataatgatc aaccagtatg gttgcccatt tttatgccaa ttttcaattt gcatgtaaat
      361 gggcagtgca gtgtcttaag aactggttat attataccat ggatgtgcga cttacccaat
      421 gaattatggt atgctttata tagataaact aagggagttt tttaatgtct aaaattaaac
      481 aaacagatat tacaaaatta agtcaattag aaaaacaaat tgcacaactg aaagagcaaa
      541 agaaaaaaca agaagaacaa ctatctaata aaattggtgg ttatttttta aataaacttg
      601 atttaaatat gatcaactct agtgatgaat tgtataaaat tatggataaa gtcattggag
      661 aatttaataa agatatcgaa caaaatagca gcaacgagaa caaggaaaat ttaacagaag
      721 acaaaattaa ctagaatagt aagtgatacg atgaatgatt ttaaacatct caaaaaaata
      781 aatgctgaaa ttgaaaaagc agaattaaga aagcatagat taaaaaattt agatagaaaa
      841 gaaagagcac atagactgat tcgaaaaggt gcaatgcttg aaaagtattt tgaatgtgag
      901 catttaagtc ctgatgaaac agaagaattg ttaaagatgt attccaacta cattaataca
      961 aataaaccca ataaatataa aaagaagtaa tttatcatgc ttacaaatac ttgggtttca
     1021 ctaaaaagaa aaaattgctg atagtagtaa caatgaataa taattgttta taaaaatggg
     1081 actaaaaaag aaaagtttta gtcccactta cttaattaat tacatttcat tgaaaataaa
     1141 gttcgcttac agttaagaga acatctaatt aaagatattc tctaattgga tcattgttgg
     1201 cttattaagt tgtattattg attaaataaa gaagtgattg gtaaatgatc acgtcgttta
     1261 ttaagcgtat gtacttctgc aaattcagat ttaagagatt tcattaagta cccttgcatt
     1321 gcttcaacgg tttcattttt ttgaattaat gtagctttaa aacgcttaag aacattaaca
     1381 atttcaaatt ccatatcttc taataaataa tatgcatcaa tagaattgtt gaaggatgtt
     1441 ttggctttta ataacacaga tttaataagt ttaatttcag atacagaaaa gttacttaaa
     1501 taactttgaa tactttgtgg tagttcttca agtaattgaa attttaaagc ttcatcattg
     1561 aattttgaat agtagtgatt tgaatgattt gtatgattac tagaatatgt taatttccta
     1621 tcattcaaat catacatatc attataatta gtcttaatat aatcagtgtc attataatca
     1681 gtatcattag actgtgaatt tggaagttct tgaccactta aattcacagt tctagaactt
     1741 ccaaattcat tgttctggaa ctgtgaattt ggaagttcct tgatatgact ggcttcaatg
     1801 tcataattag gttttaatag gtataatcta tttggtttat taaggccaac acgttcttga
     1861 gttagtaaat taaattttat taactctttt tttaatttta taattttttg ttctgcataa
     1921 cctaagtatt cacacaaatg tttattactg aaaataaagt aaatattatt atttttatct
     1981 acccattcat tttggaggga taattcaaaa cgatctttta ataacgcata cataattttg
     2041 gcttctgcag acattttctt aaaatatgca tcttcaaata aatatttagg cagttgataa
     2101 aatctttcag tttttacttg tgataaatta tatttttcaa aattgggcat aataaaaacc
     2161 tcatttccat tttgggattg aggtaataaa acaattttta cgtaagttat atgttttgtg
     2221 cttgcactta gatgattaga tatatataat acaagtatca aaaaaatata taaacaaacg
     2281 agtgcgaatc gttttattac cgagagtcga tgctacgcca atagcatcga ctcttttaat
     2341 atttataaag taatattaac acttttaatg gcgtatataa agagttttaa atgtaattaa
     2401 aagtacacct aaaactacca aacgtataat tcgctatttt agcacctaat taagtggcga
     2461 aaatatttta aggatagata attaggcatt taggtagtta atagatagta aatttagtac
     2521 taaatgatga aataaatttt gttaggtggt aaaatttagg tactaaatta ggtacctaag
     2581 gggggatgtt atgaaaacta ttaaaatgat tgctgatgaa ttaggtgtaa ctaaacaaac
     2641 tattgttaat aatgctaaaa gattaaatat atcttttgaa aaagaaaatg gaattaatta
     2701 tattaaagat aatgattatt taaaaattat agaaaaaatc actaagaaag agaaaacaac
     2761 tcataataaa gaatcagtaa aaaatgaatt atttaatgaa aatactgcaa aagttgaaaa
     2821 gtatatacat aataattcaa atagttctga gacattaaaa acaaaagtaa acgaattaga
     2881 aaagcaaata gaaatctttg aaactagagc taaaaatgat gaaaaatata ttgagaatct
     2941 aactaaacaa ttagatcaac aaaatagcaa tgttaataca ttaaataaac tgttggagaa
     3001 tcaacaaata ttatctttag aaaacaataa aaaaattcaa aaactagaaa gccaattaga
     3061 agaagaaaga cagcttaaat attcttttga tacaacgatg aataatagac agaatattaa
     3121 tccacaagaa gcaactttta ctgaagaatc tcaaaatatc aaccaacaac aaaaagaatg
     3181 tcaaccaacc gaagtaaatc ataaaaatat aactgaagag aataagcatg acagtacaga
     3241 tgaattaaca aaagaggata agtttgttga aaaaacgaag aacgattcca gtgataatag
     3301 ggaggtagaa ccacctaaaa agagattttg gagtcgttta tttggtaact aattgcaatt
     3361 aaaaaggcac acaagttcat gaacttgttg tgccttttta atgtgaatca aacttctgat
     3421 aatcatcagt tatgttaaac tctaacaaaa actaaattat cagaagaatt atctagggta
     3481 ttaaacatag gtaaatcaat tttctatcgc caatagtata aacatcatcc atattaatct
     3541 gaagatagct acataattct ttaacagcat agcctttatt tgtataaggc gccataaact
     3601 ctattaaata tggtttgttt tgaggtttct atgtttaatc atttaatttt tcattatata
     3661 aatcgaataa ttataaaaaa tcatcatact ttttattttt taaaaatatt tgaaaattta
     3721 atagtaatcc aattataaga tcaaatatca ttatcattat aatcaaggca actataatca
     3781 ctgtgacact cgttacataa caagctagta atctccagat aaaattgaca ttttcaccaa
     3841 ttccataact taacatatta caaagtagta aaagaataat aatacctata ctgtaaatta
     3901 aaattgtcag tgtatatttt ttaataactc tattaggaaa caatagttca tctattaggt
     3961 tctttttcac cagtatctct cgttcaattc tatctttgtc acagattttg attttactat
     4021 tttttatttt ttcctctaat aaaataacct cgaacgatct gctaattttt aataaatatg
     4081 caggaaaagc taatgttaag cataatgtaa ttaattcatt tacattgttc atgtaactta
     4141 taaaatagat gaaagttgct actagtgctc ctaaaaatat agttatatta agttctacat
     4201 caaatatttt aaaaatatct gctctattca tcagttaatc atcctttcaa ggtttattaa
     4261 tactaataaa ttattagata taggtatatc atattattaa tttaagaaat tgtcttttat
     4321 aattttactt aataataaaa aagtagaacc atttaaaatt aatggttcta ctttttaact
     4381 attactaatt ttaaaaataa actaatctac aatatctaaa aatatatgtt tagtactatt
     4441 tttgtttagt tatatcaagt gatttgggaa ttaacaaata gacaactatt gaaatgataa
     4501 tcatgataat cccacctact aaagcagttg ctacaaaagc atcattaaat gatgttacag
     4561 cctcgtttgc aagctgtttt atccccgtag ctttagctac ttcgacagcg cccactacag
     4621 attcttcagc tacatgagct aaatctccaa ctataccttt tgatgaaaaa gatgaaatat
     4681 ctaaaaatac acgataaagc atagaagata ggctaccaag tactgctaca ccaaaaacat
     4741 ttccaaggtc atacatagac tcttcaacag cagctgcatt acctgctttt gatgtaggtg
     4801 tttctaacat tattagagca gatgcaactg ctagtgaagc cataccagct ccaactaaaa
     4861 ttaatgctaa agccattgta gaatatgata atggatgacc aaagaaatac ataataaaca
     4921 tgccaatggc tgcaattcca attccggaag gtaacactat tttcggtcca aatcgcgccg
     4981 ctaatccggg tgcaattggt gcaaacacca tatctcctat tgccataggt aataggtata
     5041 agccagcttt aaaaggagaa agttcttcca caacctgtaa ccattgtgaa gctaataaca
     5101 aaacagatgc cattgcaaaa attgtcataa atgcagcaat tgtaccagct gaaaatgatc
     5161 tctttttaaa aagtcttacg tctaacattg gatcagaact tgataaatta cgtttaacaa
     5221 atatcactat catggtaatt gctaatacta ttacaaccca tggaataata tctgctagtc
     5281 cttcttttga aaattctttg atactccata ccagtccaat catgcctgca attgataaaa
     5341 ttgtagaagg aatatcccac gagtgagact tttcttttga taacttagac tctggtaata
     5401 aaaataaacc tgctacaact gctattatcg caaacggtac attaattaaa aatgccgagt
     5461 gccatgaaaa ttgctcaagt aaagctcctc cgataattgg tccaaaaaca gcacctatcg
     5521 atgaagcgat tgaccataca gctaatgcag tggccctttc tttagggttt tcaaaaatta
     5581 ctcttatcat tgaaagagta gttggcatta ttaaagcacc tgcaatacca agtaaaaatc
     5641 gaatagctat tacgaactct gcactttctg cgaaaaatat agctaatgaa acgaggccaa
     5701 ataaagcaaa tccagttaat aatgcttttt ttcttcccca tttatcagca aaggcactca
     5761 atggaattat aaagccagct aaaacaagag agtatatatc aactatccat aactgttggg
     5821 taccagaagg ctctaactct cttactaatt ccggtaaagc cataattaat attgtcatat
     5881 ccattgtaac aacaaacaaa ctaacagcta atactactag tgcagtccat cttttttttg
     5941 atgtcatcat atcagtagtt tttgtaaaaa atgaaatcat ttaagttccc tccaatcctt
     6001 atagaccgtg cgatcggtct ataaggatta taatctattt tttttacata ttacaacaaa
     6061 aaatcttgcg aaatttaaat ggctgtattg tgttatacaa ttattctttg aattttttgt
     6121 gctatcattg atagtactta atactcatta aaggcgtgat gaacttgaaa gataaaatac
     6181 taggtgtcgc aaaggaatta tttataaaaa atggatataa tgccactact actggagaaa
     6241 ttgttaaatt atcagaaagt agtaaaggga atctttatta tcactttaaa acaaaagaaa
     6301 atctattttt agaaatttta aatatagaag aatctaaatg gcaagaacag tggaaaaagg
     6361 aacaaatcaa atgtaaaact aatagagaaa aattttattt atataatgaa ctatctttaa
     6421 caaccgaata ctattatcca cttcaaaacg caataattga attttacact gaatattata
     6481 aaactaatag cattaatgaa aaaatgaata aattagaaaa caaatatata gatgcttatc
     6541 atgtaatttt taaggagggt aatttaaatg gcgaatggtg tattaatgat gttaatgctg
     6601 ttagtaaaat agcagcaaac gctgttaatg gaattgttac ctttacacat gaacaaaata
     6661 ttaatgaaag aattaaactt atgaacaagt tctctcaaat ttttttaaat ggacttagta
     6721 aataattata tatatattct tgaaatacca ataaatatta tttctaaatt acgttcgttt
     6781 ttgacacaaa acctcttaac ttaatattaa gaggtttttc ttggatattt aagtctataa
     6841 acttgtgtta taatcaatgt atcataaata tcgaaaatca tgagttttta gatgctgaca
     6901 gacgtgttta tttacatcga gaactatatt tcattagtaa gtacccataa aacgattaaa
     6961 aaaggtggtg ttcaaatggc taaaattggt tatgcacgtg tgtcaacaca agatcaaagt
     7021 cttgatggac agattgatac acttgaggaa tatggttgtg aacgtatctt tagcgagaaa
     7081 gcgagtggtc gtaaaactaa aagaacggaa cttgataagt gtttagatta tttacgtgaa
     7141 ggagatattc tagttgtcta taaattagat cgtcttggac gtacaacaaa acaattaatt
     7201 gagctatctc aatggttgga tgaaaatagc attgatttac atattataga tatgaatgtg
     7261 tcaacgaaag atgcgatggg caagatgttc tttaccatga tgagtgcatt cgctgaactt
     7321 gaagctaatt tattaagtga acgaactaaa aaaggtttgg aagctgcaag agcaagaggg
     7381 agaaaaggtg gaagaccttc actaccagat cataagaaaa gagagatcaa attcttatat
     7441 gatgaacaaa agctgtctgg tgaagaaatt gctgaacaaa caggagtgag tcgttctact
     7501 gtatatagga ttattaaaga gtcaagaaaa atataaagta ctaaattaaa gttttaatat
     7561 accctttaat tgaaaaatct tcttaaaaag tgtctttaac ttcagtcccc tttaacaatc
     7621 cactcgctta atagctttaa atttctaagc attacttgag ctttgaaaat tttcatcgat
     7681 tcattaaccg acataactgg attttgactg ttaatattca ccttgtcctc tccaattaac
     7741 aaaaaagcaa aagcatataa acaacatttg ttgtttatat gcttttgctt gcattttctt
     7801 ctatagtatg ctataatgaa aaggttaagt ctatctatac atcaaaagca ataaacttcc
     7861 acctagaata acaacttatt gtaaatcgac aattacaata atacatcatt ctatattgga
     7921 agtaaacaaa aaacttacag aaggatgata tagagtgaca ctagtatgta ccaaattcaa
     7981 tcatgatgct aaatcaactg attatttaaa tatttctatt gaagaagaca ctcttcaata
     8041 tatagaccca aaccttatta aagtaatatc taatagagaa gttggaaaag ttgcgacttt
     8101 ggctcaaaga gcacaagagc aaattgatgt tttttttgaa aaaattatcg atatacatct
     8161 tagtgatttt tctgataccc aaaagcgtaa tgaatttaga aaattattct ctcatttttc
     8221 agaaccacac catttacgct taggtttttc tcagaaaggt aatacaggaa aaggtactac
     8281 agcatctgaa ttgattaatc tatttatgaa tgaagatatt ctttccatta tcttaaatga
     8341 tgacggatta acaatacctc aaaaagcacc attaatcaga aattttggtg atgataaatt
     8401 atcagattta acaagtaata taatcatgaa tgttataatt gattttaata aatccatgat
     8461 tcagtcattt ccagaaatga aatcgtttct ttctaaagaa actgtgacat attattattt
     8521 ctctactaac ggtatatggg aaacttatga tttcactcca tttctttttg atggtaataa
     8581 aactttatta gttccaaaat tattcactac ttataatcag acatctagtt tagatttgat
     8641 gataagagta tacattgaag aagaaatcat tagattgcaa aaacaacgta atacaaccaa
     8701 aaaaatgaca aaaaaacaat ttatcaatga atttataaaa ggacatcgta tggataatat
     8761 gacctatttc tttttaaaca cttcttcaaa atcacacaaa aaatttcaaa aagaattaga
     8821 ttcaaaagcc aatgaaaggg gtaacaataa caaagatgat aattagtact tactaatcag
     8881 tatagtatag acttaacata ctaattattc aattttgcat tcctccacac tatgatgtta
     8941 taatttagat aacaaatatg tgttgaacat ggtaaacacc gagcccgata actattccag
     9001 tagttatcgg gctctttttg gtgttattac tgtgtcgtcg ctttattgtt tatcacgatt
     9061 attcaaccaa tgtgaaaaca acgtgattat aacccctact ataataggag ctactataga
     9121 tacaaatatg atgctgaaca tggtgtcacc tccttccatg ctaaaccagc acagaagtag
     9181 aacgacgact tttatattat atcatttgtg aattgttaag catatgcact tccgataagt
     9241 cattttatgt ttaatgtatc tatgtgataa tgaaaataca aattctatta aaggagataa
     9301 ttttatgaaa aaacgatcat tacttccaac tttattattg gctagctcat taacattcac
     9361 aggtttttca gcgacagcca atgctgagga tggtgccaaa accgtatacg gagagaactt
     9421 accaaatagc gcagatacaa atggagatgg ttgggctaat gtagacttca atacttcatc
     9481 cctcccacaa tctgcacaaa atcaaataag tgaactttct aaacagaaag attcagggga
     9541 attaactcaa atcgagtata atgagaaagt ttcaagtgta tttaatgaat atcaatcaca
     9601 aagtactgat atacaaaata atgagagaac tcaacaaggt aaaagacctt tcggaggggt
     9661 gatacctaat tgtatgactc aagaagaata ccaagaatta gaaagtcatg ttcctaaccc
     9721 taatagtgta tccacagaaa aatataatca aattgtaaaa aatgaaagtc aaaagatagc
     9781 taatgaagaa aataaaacta ttaatacacc tcaaggtgtg ttgacacctg taactgaaaa
     9841 taaaaacaag agttctgaag gaatgaaaaa tcaagattca aactctcaag tattaccaga
     9901 aacaggagaa agcaaccaaa gtgatatatt gtcaataatt ggcgcttctg gactactaat
     9961 attaggtata agtcttttaa tattcagaaa aaaggcaata taaaaattga taaaataacc
    10021 accgtgttta tgtaaaacat gtggttattt tattatatat ttaatacata tttttcaact
    10081 catgtaattt acgttttctt gctgaaaaaa tattaacata atctaatttg aaatactgaa
    10141 aaggagttgt aatatgaaaa aaatcatttt acctatatta attgttagtt tatcggtaat
    10201 tttagtgagt tgtaataata acgattcaga acatcacaat gataataatt ctagtgatac
    10261 taacacctca gaaaacaaag ataatgaagc atcaaatgaa attgacgaaa aaactaacac
    10321 atctgatagt gcggataatt cagaaagtag taataaacaa aataaagaaa aaaatgataa
    10381 taaaaaacaa gaaaaaaata atgatagtaa taagaagcaa caatctactg ataaaaataa
    10441 aagttactta aataatttta actcgaaaga aattgaatat gcacgagttt ggtatcagtt
    10501 aatatctaca agaaatgatt taaaaggtat taaaaatgtt tatgttacag aaataccaaa
    10561 aggctcaaaa gtaaatccac aagccaaaaa tagtgcaatt tataaagaaa aagttgtgaa
    10621 attagaagct cctatgagag ctggtgggtc tgtaacatat agcagtaatg gtgatggaac
    10681 aattaatgta tataataata tcccttataa atgggaaaat tcacaaaata gtgattatag
    10741 tcaaatgaat aagataacaa aaaaagcaat agaggagaat attgaaacag tctatattaa
    10801 accctacgac aataaaacag tagctaagtt agcaagtaaa attagatata ataagtagtt
    10861 agtagattat taaaaaggcg cctaaaatta ggcgtctttt ttatcattag ttcaaaagat
    10921 aagcataacg ttccatgtaa cagcatataa agtaatgaat caattcatca ctatctaatg
    10981 ccaccattgt tagttcagcc ttgcatggtt taacgtatct cacataatgg tacaaatctt
    11041 caaatagtgc ctgtttttcg ggcattttaa acaccttgtg tacattaaaa cctatttctt
    11101 ctaatcgctt tgtaatcacg ccgaaaacga atgtgttcat gtgaaatcac tccatcattt
    11161 ttaatattca catgtacact aaaaaaaatc cgatacatcg gctttttatt tttggtttaa
    11221 tttatttcta tattgttttc actatcgctc ggacgcataa gtgttaaaac accttctacc
    11281 atacctatca cacccggtat tcccgtccaa caaaatagta aataaagaag agcattttta
    11341 tttttcttaa cataaaactt atgagccccg aaacctccta gaaaaaatgc taatgcagag
    11401 taaacatatt tattaacttg catgatttcc actcctttaa ttaattgatt tagagaaaca
    11461 ttaaataatt tagtgatttc taaaattgtt tttaagtcgg gattacttac ttctctttcc
    11521 caattagaaa tagaatgtct cgatacccct aacttttcag ctaatttttc ttgagacatg
    11581 tttttacttt ttcttaactt ttgtatttga tgtccaattt ttgtttccaa attatttttc
    11641 tccttaactt taagatatat ttaaactcaa ttttttctat cgatatttat gaacattagt
    11701 attttttatg ctgaaatatg tgaacaatcg cataatatca aggtttttct ataattatac
    11761 acgttttaaa atacattgtg ataatacata gaatgtacct tatctaagta tataaatgtt
    11821 ttctttaaac caaagctaat ttaccacatt gggttaagtt tcttattatt aaagaatgaa
    11881 ctttgtatga tttcaactgc ttactatctt ctcatttggt tatttctctc ttgtttcttc
    11941 ttctttttct gatgattaat attgttttgc ttttcagttt cgttcgcata gtacataaga
    12001 aagtcactag catttatcgt tggtaaatta atgtgattag tttgttcatt ttcatgttca
    12061 atacgattgt catgatcatt tctatctact tgattaatat gtttttcgat aacttcttct
    12121 tgatcgacat gattatattt ctcaagttta atgagtaatt gaactttttc attaggtgtt
    12181 tggtctttga ctttttcaaa gtttttatcg cctatatcta ttcttaaatc atcatcaata
    12241 tgttctttat aactatcaat ttctttttct ttttccttaa taaattgcaa ctcaacgtta
    12301 tattcaaagc tatctttttc taaattttta agatgttttt gactaaagaa aatatcttta
    12361 ctcgcttttt tataagtatc ttctagcaat acatcattag gaatattgtt attttgtttg
    12421 aacaggtatt gaacatgagt tgcttttata atattacctt tttgtgcctc atcaacaata
    12481 aatgcttttt catcatggct aaaatgatga tgagatttag ggtacatttg ctgaacaatg
    12541 agatgatttt tttcactttc agttagataa acatctttag ctgattgata aatctcttta
    12601 cgtttttcct ctctactttc taatttattc ttctcattga gtaacgcttt atagttagtt
    12661 tcaacagtat taacaaactc ttgttgacta tatcctttac tttcaatttc ttttctaaac
    12721 tgataatttt catcataatt tttagcatag aattttaatt gacgtctctt aatttcattt
    12781 ttaagtaaat cattttgaat tttacgacca aaggtagacg cactgtcaaa cgtattttta
    12841 attgtttctt tcgcctctga atagccaaca tcacgttgat atctatcaaa taaaatagaa
    12901 taactttgtt catttttact tacttgttcg ttagcatttt gaataaataa tctaatttca
    12961 ttttgtgctt gttctttctc tttatcacta aataattgtg atacttcgcc acgattatat
    13021 cttctaattt gttcattttt ctgtccgtaa taagtaacgg gttggtactc gataccctct
    13081 ttttcacagc gttttctttc attttgttct aatctatatt cttctcttgt aagtggaatt
    13141 tcggcaattt tttctaaacc ttgcgcttca aaagaacgac tatcatatcg tcttaaactt
    13201 tgttcacgtt ctgagtattg atttaatttt aattcccaat gcttacgaac ttctttgaaa
    13261 tcaaagtcgg caaatcgttc ttgttttcta actttattac catttttatt taaaatcgca
    13321 ttgccatttt catcatattg cgttctcgtt tttgtctttt tcccccaagt accgtctgca
    13381 ttaaacggtc tttgagtaag tagaacatgg gcgtgtgggt tgtttatatc atctctatga
    13441 attgaaatat cagcaaccat tcctttgtct acaaactcac tttttataaa atctttagtt
    13501 aattcagttt gtacctctct atcaaaatca ttaggcaaac ttaataagac ttcttttgct
    13561 aatctaggat
//
