LOCUS       NZ_CP073796             4947 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae isolate KP_NORM_BLD_2010_71621 plasmid
            unnamed_5, complete sequence.
ACCESSION   NZ_CP073796
VERSION     NZ_CP073796.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8602826
            Assembly: GCF_019798185.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Fostervold,A., Hetland,M.A.K., Samuelsen,O., Sundsfjord,A.,
            Simonsen,G.S., Bernhoff,E., Bakksjo,R., Wyres,K.L., Holt,K.E. and
            Lohr,I.H.
  TITLE     A nationwide genomic study of clinical Klebsiella pneumoniae
            isolates in Norway 2001-2015
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Fostervold,A., Hetland,M.A.K., Samuelsen,O., Sundsfjord,A.,
            Simonsen,G.S., Bernhoff,E., Bakksjo,R., Wyres,K.L., Holt,K.E. and
            Lohr,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2021) Department of Medical Microbiology,
            Stavanger University Hospital, Gerd Ragna Bloch Thorsens gate 8,
            Stavanger 4011, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 58x
            Sequencing Technology             :: Illumina MiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:34:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,516
            CDSs (total)                      :: 5,396
            Genes (coding)                    :: 5,271
            CDSs (with protein)               :: 5,271
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 54 of 125
            Pseudo Genes (incomplete)         :: 81 of 125
            Pseudo Genes (internal stop)      :: 32 of 125
            Pseudo Genes (multiple problems)  :: 33 of 125
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073796.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="KP_NORM_BLD_2010_71621"
                     /isolation_source="Blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed_5"
                     /geo_loc_name="Norway"
                     /collection_date="2010"
     gene            join(4074..4947,1..41)
                     /gene="lpxO"
                     /locus_tag="KFO61_RS27435"
                     /old_locus_tag="KFO61_27435"
     CDS             join(4074..4947,1..41)
                     /gene="lpxO"
                     /locus_tag="KFO61_RS27435"
                     /old_locus_tag="KFO61_27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_222169323.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWWWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            complement(760..927)
                     /locus_tag="KFO61_RS27440"
                     /old_locus_tag="KFO61_27440"
     CDS             complement(760..927)
                     /locus_tag="KFO61_RS27440"
                     /old_locus_tag="KFO61_27440"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_222169324.1"
                     /translation="MTRDKNDRESPEEPADRGTRDDKSSQNDKGGDWIGELERELQEIT
                     KKWPGLRSLS"
                     /name="hypothetical protein"
     gene            2083..2406
                     /locus_tag="KFO61_RS27445"
                     /old_locus_tag="KFO61_27445"
     CDS             2083..2406
                     /locus_tag="KFO61_RS27445"
                     /old_locus_tag="KFO61_27445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_192869793.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNGGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2615..>3049
                     /locus_tag="KFO61_RS27740"
                     /pseudo=""
     CDS             2615..>3049
                     /locus_tag="KFO61_RS27740"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3084..3581
                     /locus_tag="KFO61_RS27455"
                     /old_locus_tag="KFO61_27455"
     CDS             3084..3581
                     /locus_tag="KFO61_RS27455"
                     /old_locus_tag="KFO61_27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_142674820.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSELEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAAILWWQGNQML
                     DNYASIREQRDALEKLNARTWGVRYQEDNQGRFLVLPEGVKADTNWTFDNGTKIGIRLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            3588..3803
                     /locus_tag="KFO61_RS27460"
                     /old_locus_tag="KFO61_27460"
     CDS             3588..3803
                     /locus_tag="KFO61_RS27460"
                     /old_locus_tag="KFO61_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1797..1980
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.702"
                     /coverage="86.0"
                     /evalue="1.25e-50"
     Typing          complement(1339..1814)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.627"
                     /coverage="92.0"
                     /evalue="2.73e-170"
     Typing          2397..3901
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.867"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttttgtttg gcttgctgtc tcagtgttta gatggctgtg atttgttttt ttctattgag
       61 tcatacataa atttgggtct tctatgctca atgtatggtt atagataaaa atcgcctgct
      121 aaaaaattct attgaaatct ttttagtaag aaaggcccga atctgacgct cagcgggcct
      181 ttcgtgaaga agtgcggcag tagacagatt tcatctgatc acgttcatgt gctgctgtgc
      241 aattaagcgt ttctttcgga gctttcacgg tatttccttc acgaaaccgg ccgccattca
      301 ctcttgtgca tagccatgca gccgcgcaga aattatcagg ggcgtttttc agggtttggg
      361 ccggggaaag gtgccggaaa cggccctcag gccgcagaaa cgcgctgagg ggctctcagg
      421 gggtgcggcc aagagatgtt atgttaaaag cgtgacgccc ggcaggtacg aagaaaaaag
      481 atggcagctg cgcttatgtg cctgcagaga aggtcgataa cggtagtgtg cctcaccaga
      541 ggtgcataaa ccgatactgc tgctgtatgt gctgaaggct cccgctgcgg ggagcctttc
      601 ttttgccctg tgcggtctgc ggtggacttg cggaagcgcc tttttattac acactttaag
      661 tgtgccgttc catgctggtt tcggcacgtt gctgtgtatt ttgaacgcgc tacgtcgttc
      721 agcccgctac taaatatggc gggtttttac ggtcatgatt cagctcaggc tccgtaagcc
      781 tggccacttc ttcgtgatct cctgcagttc ccgctccagc tcgcctatcc agtccccgcc
      841 tttgtcgttt tgtgagcttt tatcgtcgcg ggtgcctctg tctgcaggct cttccggcga
      901 ttcccggtcg ttcttgtcac gtgtcatctg tgtttccccc ttcagccccg ggatattttc
      961 ggcgggaagg ggttacactc atgcgcgaag cgggtgaagt tatgaccagc attcagagga
     1021 atgctgcagg ggttgagact gcggttaagg atgtcgccat ttcagttaaa gccggatagg
     1081 ccatggtgca tatccccata tctgtcgaac atcacagatg atcttcttga gatcctttcc
     1141 gttgcgtcgt aatcttttgc tctgaaaacg aaaaaaccac cctgcaaggt ggtttttcga
     1201 aggttattta atcctggcag attatctaac cgcggtaaca gtcttgtgcg agacatgcca
     1261 ccaaatcagt ccttccagtg tagcctcagt taggccacta cttcaagaac tctcgataca
     1321 tctctcgcac atcctgttta ccaatggctg ccgccactgg cgatttttcg tgtctttccg
     1381 ggttggactc aagttgatag ttaccggaat gggcgtggta gtcggactga acggggggtt
     1441 cgtgcataca gtccagcttg gagcgaactg ccttcccgga actgagtgtc aggcgtggaa
     1501 tgagataaac gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga
     1561 gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc tgtcgggttt
     1621 cgccgccact gatttgagtg tcagatttcg tgatgcttgt caggggggcg gagcctatga
     1681 aaaaacggca acgccgcatc ctttttccgg cttttcttga gcgccatctc cttatctcgt
     1741 cattcgtgag caggtaacgc ccgccgcagt ctcacggcag gagcgtagcg accgagtgag
     1801 cgaggaggcg tcatttcacc tacaccggca tttcgcactt acgccccaca gccgcatttt
     1861 ttacttacca caggaagcac tttcctgaat tcccacttcg acctaccata ttaagccagt
     1921 atacactccg ctagcgctac gtgactggtt caggcctgcg ccccgaaacc cgctaaaacc
     1981 actgacgcgc ctgcggcttg cccaacaccg cgccgaccgg aaaaagattt cccgccgtcc
     2041 cggcgttatc aggaggccgg attggcagac aaacgcagca aaatgctcac gatgtgggtg
     2101 accgaagatg agcaccgccg tctgctggaa cgctgcgacg gtaaacagct cgcggcctgg
     2161 atgcggcaga cgtgcctgga cgagaagcct gcccgcgccg gcaaactccc ctcgatctcg
     2221 ccggcgctgc ttcgtcagct tgccggcatg gggaacaacc ttaaccagat tgcccggcag
     2281 gttaacggcg gtggcggcag cgggcacgac cgcgtgcagg tagtcgccgc actgatggcc
     2341 atcgatgccg gactcgagcg gctgcggcat gccgtgctgg aaaagggggc agacgatgat
     2401 cgttaagttt caccccaggg ggcgcggcgg tggcgctggt ccggtggatt atctgctggg
     2461 caaagaccgc cagcgcgaag gtgccagcgt tctgcagggg aagccggagg aagtccggga
     2521 gcttatcgac gcctcgccct acgccaaaaa atacacctcc ggcgtcctgt cttttgccga
     2581 acaggactta ccgcccggcc agcgtgaaaa gctgatggcg agcttcgagc gggttctgat
     2641 gcccggactc gataaagatc agtacagcgt gctgtgggtt gagcaccagg acaaagggcg
     2701 gctggagctg aacttcctga tcccgaacac ggagctgctg accggcaggc gtctccagcc
     2761 gtattacgac cgcgccgacc gtccgcgcat cgatgcctgg cagaccatcg tgaacggcag
     2821 gctggggctg catgacccga acgcgccgga aaaccggcgg gcgctggtca cgccgtccgc
     2881 gctgacggaa acgaagcagg aagccgccca ggcgattacg cggggtttac tggtcttagc
     2941 ctcgtccggg gagctgaaaa accgtcagga cgtcactgag gcgctggaaa gcgcgggttt
     3001 taaggtggtg cgcaccacaa aaagcagcat cagcattgcc gacccggacg gggggcgaaa
     3061 tatccgactg aagggagcca tttatgaaca gtcttttaac gctggcgaag gacttagagc
     3121 agaaatcgaa agcgcagcag cagactaccg gcgagatgct gaaagccgca ttcagcgagc
     3181 tcgagaagtc tgtcagagcg gaactgagcg aaagcgagaa gagaatcagc gccgccatcc
     3241 tcgaccacga ccggaagctg tcctcagcca tgagccagcg cacgaaaggc atggtgcgta
     3301 tggtcagcca gacgtggctg accatcgtcc tggtctccgc gctactgata gcctcgagcg
     3361 cggccattct gtggtggcag gggaaccaga tgctcgacaa ctacgcgagc atccgggaac
     3421 agagggacgc gctggagaag ctgaacgccc ggacgtgggg cgtgaggtac caggaggaca
     3481 accaggggcg ttttctggtg ctgccggagg gcgtgaaagc ggatacgaac tggacgttcg
     3541 acaacggaac gaagatcggc atcaggctgg tacgggagta gatcagaatg acagagctgg
     3601 aaaaacagtt gctgagcgca ttagagcagc tacagcagga ctactcgaaa aggctggacg
     3661 agtgggagag cgccttcgcg gaatggcgga cgatgtctgg tcttatgcaa cgggagaacg
     3721 cggcgctgag cgagcgcgtc acgggcttga gcaggcaggt gcagagtttg agcgatcagc
     3781 tgcgccggtt gtcgagcggc tgaatgccgt cgagctgcag caggtgcggg aggcgcggca
     3841 tcagaaagcg ctggagctcg aacgatcaca gcgacagcgg gtatatcacg gtccgtccct
     3901 ttgattcatt tcagcataat tcctgctgaa atcctctctg cctgtgtagt agatatgttc
     3961 tggtgaagct tcgattatac tattgataaa tagcatttag attataattt aagtgcattc
     4021 attatgttat ctttaagaga tttttattat tggcgtgaac aaggaatata cacatgctgt
     4081 cacttattat tatttttgtt tttcttatca gtgtattgta tgcgcattct cgcggtaaaa
     4141 taaaacaaaa gttttccagg cagttatttg atcattcaac atttatggct ccgataaata
     4201 tgtttatgac aggcttttcg aagctgccgt caaagcaggc atggtttccc ccagaaacat
     4261 ttcctgaact tcaaccatta attgataact ggcaggttat aagagacgaa gctgttaatt
     4321 tgcaagggca cattaaatca tctgaaaaaa ataatgatgc aggatttaat acatttttta
     4381 agcgcggttg gaagcgcttt tacctcaagt ggtatggaga tgctcatcca tcagcggagc
     4441 ggctttgccc agtaacaacc caactggtta gctctattcc ctgtattaaa gcagcgatgt
     4501 ttgctgaatt gccttcagga agccatttag gaaagcatcg tgacccttat gccggttcag
     4561 ttcgttatca tttaggatta tcaacaccaa atgatgaacg gtgttttatt gaagttgatg
     4621 gtgtacgccg cagttggcat gatggtgaag tgataatttt tgacgaaact tttgttcact
     4681 gggctcagaa tgataccgag cagacacgta ttattttgtt ctgtgatata gaaaggccga
     4741 tgaagtggtg gtggtcacaa aaaataaata ggtggatagg tggggtgcta atgtcagcgg
     4801 cagcatcacc caacgataat aatgacagga ctggtgctat aaatcattta tttaaatatg
     4861 ttaatgctat tcgtgatgca ggtcagaaac taaaaaaaag gaatcgaaaa ctatattatt
     4921 tgattaaaaa tggagttatt tctttga
//
