LOCUS       NZ_CP073795             5463 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae isolate KP_NORM_BLD_2010_71621 plasmid
            unnamed_4, complete sequence.
ACCESSION   NZ_CP073795
VERSION     NZ_CP073795.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8602826
            Assembly: GCF_019798185.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Fostervold,A., Hetland,M.A.K., Samuelsen,O., Sundsfjord,A.,
            Simonsen,G.S., Bernhoff,E., Bakksjo,R., Wyres,K.L., Holt,K.E. and
            Lohr,I.H.
  TITLE     A nationwide genomic study of clinical Klebsiella pneumoniae
            isolates in Norway 2001-2015
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Fostervold,A., Hetland,M.A.K., Samuelsen,O., Sundsfjord,A.,
            Simonsen,G.S., Bernhoff,E., Bakksjo,R., Wyres,K.L., Holt,K.E. and
            Lohr,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2021) Department of Medical Microbiology,
            Stavanger University Hospital, Gerd Ragna Bloch Thorsens gate 8,
            Stavanger 4011, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 58x
            Sequencing Technology             :: Illumina MiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:34:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,516
            CDSs (total)                      :: 5,396
            Genes (coding)                    :: 5,271
            CDSs (with protein)               :: 5,271
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 54 of 125
            Pseudo Genes (incomplete)         :: 81 of 125
            Pseudo Genes (internal stop)      :: 32 of 125
            Pseudo Genes (multiple problems)  :: 33 of 125
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073795.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="KP_NORM_BLD_2010_71621"
                     /isolation_source="Blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed_4"
                     /geo_loc_name="Norway"
                     /collection_date="2010"
     gene            complement(join(5357..5463,1..913))
                     /locus_tag="KFO61_RS27410"
                     /old_locus_tag="KFO61_27410"
     CDS             complement(join(5357..5463,1..913))
                     /locus_tag="KFO61_RS27410"
                     /old_locus_tag="KFO61_27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271364.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="replication initiation protein"
     gene            complement(1714..2838)
                     /locus_tag="KFO61_RS27415"
                     /old_locus_tag="KFO61_27415"
     CDS             complement(1714..2838)
                     /locus_tag="KFO61_RS27415"
                     /old_locus_tag="KFO61_27415"
                     /inference="COORDINATES: protein motif:HMM:NF013211.4"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_021529456.1"
                     /translation="MSGGDGRGPGNTGLGHNGGKPSGNVNGSSGHGGPSSGGGYHYNPG
                     KGPASTVGPDGEVHINITGGMEKDPPGKHHGNGGSGNGHGAGNHNSNSGGNGKTTSIPG
                     WSPDSGKPGRYLPTNKDKLIKGLIQSIDDHSGTYTIHYTGGAVATVTVPNGDVNKMNVH
                     YNIGSGPSSGNKLTRDTIKSFLEFKAENEANAVNDAISKVNDFYKELASKYGDKLEKEA
                     KALADEAKGKKIRNANEAIKAFDRYKDVLNKKFSVSDREAIAKALESLNKDQMAKQLKI
                     FGKAFGVVGEAIQWGGFISGLVKGFRTGDWNEAFISGEKIAVGKVASVMVTVAFSAMAV
                     NPIGILGFAVIMAVTSALITDERLKQLNSFINGI"
                     /name="colicin-like pore-forming protein"
     gene            complement(3059..3592)
                     /locus_tag="KFO61_RS27420"
                     /old_locus_tag="KFO61_27420"
     CDS             complement(3059..3592)
                     /locus_tag="KFO61_RS27420"
                     /old_locus_tag="KFO61_27420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168774.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQNLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVILED
                     KLAEKPVTGKNRKN"
                     /name="hypothetical protein"
     gene            complement(3595..4521)
                     /locus_tag="KFO61_RS27425"
                     /old_locus_tag="KFO61_27425"
     CDS             complement(3595..4521)
                     /locus_tag="KFO61_RS27425"
                     /old_locus_tag="KFO61_27425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001736644.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIEGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKNGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIMEWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQDRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            4698..5096
                     /locus_tag="KFO61_RS27430"
                     /old_locus_tag="KFO61_27430"
     CDS             4698..5096
                     /locus_tag="KFO61_RS27430"
                     /old_locus_tag="KFO61_27430"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_222169322.1"
                     /translation="MRISNIEWMKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     Typing          complement(139..913)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.907"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(3599..4521)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acgctcccaa gatatacata tcaagcgccc tacgacgttt gtcgtcgtta gcagtccacc
       61 gaacagcggc gctttttttc cctttgtccg actgattttc tgacgaattg gccttcccgc
      121 ccttgcgacc gcgtgctgcc tgaatttctg gcgtgtgcgt atcagccaca tactgtgcga
      181 aagtttccgg cgtgaagttc ctgtgcgtgt atttcgcaat gctcttgcca atagcccgac
      241 attcagccag tgaaagcgga acgggaagcg atgcattgta catttcgaca cgctggatca
      301 ccgcttcaag ccactgtgag aacgctggcc agccctgacg aatcgccctg taagcccatt
      361 tgcgcgtcat ttcgaacagg tggcagtttc gccccatccc gtaatgttta tcgatgctac
      421 gacgctctga ggcgctcaaa tcgagataat ctgccagttc atcgagggta taggcgtcct
      481 cgcgccactc catcacctgc cattccaggt ggaacggatt tttgcaaatc aggccgctgt
      541 agttcacatc agcacaaagt ttctcacaca gcgaacgttc aaccgctgcg gcatatttca
      601 gcgccttaac cgaggcatca ggcgcggttc tcacggcgat gttgagtgcg tagagcaggt
      661 gagcgtgtcc gttcacagga tttttaacgg tgatgttcgg cgcgggggcg ttccggtcgc
      721 tccagtcaat cgcagccccc tcgcggtcaa cgtcaaaaac cagccagaac tgcgcatgag
      781 gctggttctg ctggatgtac cgcgcaagaa gcgcacgccc tttgccggag atgcgaaggc
      841 cgaaagcgag atcatccgtg tgataggggc ggtggggtaa attttcttcg aagtattgaa
      901 gcgcggcgct catgtgttac gattccttcc aggttatcga ccgacctcgc ccctgtattc
      961 tcgtgacaat cttggcggag agacacgaat ttcaggggcg tttgtttttc agtcgcttac
     1021 actaccccca acaacaaaac ctgtcgagcc ttcccgcaaa accgatccat ttaccataac
     1081 tcccgttgta cgtacccctt aaaacgccct cagtcgcttt ctggcgcaaa aaacgcaaaa
     1141 agacaaggaa acaccaggcc aaaccagaaa aatgcgtcag aatcactttc agggcgttgc
     1201 ataactatgc acgaaagcga ttcgcaaaca gtttcgaccg ttcaaaaggg cgaacgagtc
     1261 aaaaaagtaa cttttgtgat aactacttct agatttatgg ataaagtaag gcaatattat
     1321 aaacatttaa ctaacgagga ttttataaat gaaaaaaata gatatggaat tctatttaag
     1381 gaattttatc cttgccttat ccttttacat tttatctttt atcatcgcaa gtttgaatct
     1441 aagcccaaca agtatagaat tatttatatt tatacttata tctctttttg gatcgtccac
     1501 ataccctttt tgtctattac taataaataa gcaagcaccc ttactaacaa aaacaacttt
     1561 taacttcgtt aactggggtt ttataatatg cattatatta tcactacctc catttggatt
     1621 gatttttaca gtctattact ttataaccaa caaaaagcga tagaaaaaca aaataaaggt
     1681 cataccatat caaacggtac gaccttttgt ttattaaatg ccgttaataa agctgtttag
     1741 ttgtttcagc ctttcatctg taattaaggc actagtcact gccataatta ctgcaaaccc
     1801 taaaatacca ataggattta ctgccatagc actaaatgcc actgtaacca taacagatgc
     1861 taccttgccg actgcgattt tttccccgct tataaaagcc tcattccagt caccagttct
     1921 gaatccttta actaagccac tgataaatcc cccccactga atagcctcac ctacgacacc
     1981 aaaggcctta ccaaaaattt ttaactgttt agccatttga tctttattaa gagactccag
     2041 tgcttttgca atcgcttctc ggtcggatac gctgaatttc ttattaagca catccttgta
     2101 cctatcaaag gctttaatcg cctcattagc attgcgaatt tttttccctt tagcttcatc
     2161 ggctagtgcc ttggcttctt tctccaactt atcgccgtat tttgatgcta actctttata
     2221 aaagtcatta actttgctta ttgcatcatt tacagcatta gcttcatttt ctgccttgaa
     2281 ttctaaaaag ctttttatag tatcacgagt cagtttatta cctgatgacg gcccactacc
     2341 gatattatag tgaacattca ttttattcac atcaccgttt ggtacagtta ctgtagctac
     2401 tgcaccacca gtataatgga tagtgtacgt tccagaatgg tcatctattg actgaataag
     2461 tcccttaatt aacttatctt tatttgtcgg taaatatctg ccaggtttcc cagagtcagg
     2521 gctccatcca ggaatagacg ttgtttttcc attgccgcct gaattgctgt tatgatttcc
     2581 agcaccgtga ccattacctg agccaccatt accgtgatgt tttcctggag gatctttttc
     2641 cataccaccg gtaatgttga tatgcacctc cccgtcagga cctacagtgc ttgcaggacc
     2701 ttttcccgga ttgtaatgat acccaccgcc agatgatggt cctccatgtc cagatgagcc
     2761 attcacgttt ccactgggtt taccaccgtt atgtccaagc cctgtattac cagggccacg
     2821 accatctcca ccactcataa aaattccctc ttttaatcgt taaaacaatt aattacatag
     2881 ttaatactgt acataaaacc actggtttta tgtacagatc aaaaagtact cgccattatt
     2941 ttttctgtca agtcagggag ggatattttt tggcagttcg gaggaatcac tcatgaaaac
     3001 ggtgaacgga acgcgaaatc ctttcgctgt ccgtgaggcg ttggacaagc gaagcgcgtc
     3061 agtttttccg gttcttaccc gtgacaggtt tttccgccag tttatcttcc agtatcacaa
     3121 ctttctgata caccgccgct gtggcgttgt agttcctgtc gagcattgtt ttcattcccc
     3181 atccgagcac acagaacata actagaaaag ccactgacac cccacccagc catttccagc
     3241 tatacccttt aagggtgttg tattcctgca cgatattgct gtgaattgtc gcaatatccc
     3301 tgttcagggc atccgcgttt gccttcatgt ttgcgactga ctcttccagc cgctgaaaat
     3361 cctggctgat gcgctcacct atcagccctg ctacgtccag attctgcacc tcatttctga
     3421 cttcatccct gacctgtttt gccacaccgt cagaaatatc ccgatatccg cgaagtgttt
     3481 cccttgccac agccagagaa tcagcaacag ctttctgttg ctccattgcg gtatgttgca
     3541 aatgctccac cgataacagg agcgtgagta atttttcttc tgtgctggcc atgttcagaa
     3601 tcccatagaa aatccacgtg acggtgattt aacccgttca ggttcagttc ggtcctgtct
     3661 gctcactctg tctctcatgt caaagccatg ctcccgcgca acatcatgtt gcagccttct
     3721 gaccagttca tgttgcttaa ccggagtgtc cagccattcc atgatgtcat gcccgaccgt
     3781 tcgcgcctgt tcaagatggc gggaatagtc cggttcagac cgtttttgca tgatgtcatg
     3841 acgctgggca tcatcaagcc gtccggcagc ttcaaaaccc agatgcactt cgggacgtcg
     3901 ttcaatgccc tgttctttca gcgtccgaca gtcaatgaag cccctttcaa agccattttt
     3961 tctcatatgt tcgttgtgct tcacctccca gcgttttcgc aattcccgca actgctcttt
     4021 ttgctgagtg ggagtcaggc ttcctgaatc ctttttcgca cctccccgct cgggatacct
     4081 ggcgttatag cgccggaaat actgttccgg tgtacgttca atgccatcat tcacacgctg
     4141 actcatcatg atatgcgcat gaggctgttc gcctccctca atgctcgctt tcggattgtg
     4201 aatggcaaaa ctccatgcat gccgttcgcc agcttcctga cgtacaaaat cctgcaccag
     4261 ttcaaggcgc tgttccggtg tcagttcacg gggcagcgct atctccagtt cccgataagt
     4321 cgaaccgttt gcacgttcaa actggtcagc cgcctgccag aaatgggagg gattatctcg
     4381 cgcccattcc ggcatgtttc cgtgtgcgga atattccaaa tcttgacggt cgcggtattt
     4441 ctctttgcgc tcgatgtaat cggcatggtt tgcagcctgt cccttgccac caaacttaac
     4501 gctgaggtga taggtcgcca cggtttcagc cctccgcagg agcgcactgt ttctcgacga
     4561 aggagagaaa catataagtg cgcttccttc taattttacg aattagcacg gaagataccc
     4621 cctgcacccg ccgaccgcaa gcgatatact gcacgaccac aacacaccgc aaaaaaacac
     4681 cagggagcac cacgaacatg cgcatcagca acatcgaatg gatgaagaaa cggatcggat
     4741 tcatcaggaa gctcggagaa cagaccgcac gacagagaca gattattgac ctgctcgaca
     4801 acgaagcagg actcacagaa caggaacgca aactcctcca cgtactggca acagcggaaa
     4861 aaaacgactt acaggcacag gagagcgaac gaaaacaggc ggtccaaaaa cgaatcgagg
     4921 gaaaaaaaca acgacgagaa cgcaatcacc gactctttct cgcggctggg ctactgatcg
     4981 aagcaggact ggtagacaca aaaacaggcg agctatgcta taaaaaagac aggatattac
     5041 aggcacttaa ggaaattaag tacgatctgg agacatcccc aaacccagac gcgtaagtgc
     5101 ctccatccgt gttccggttc cctggtgctt cctcgctcat tcgctcgcta cgctcggtca
     5161 tgagactgcg gcgagcacga ccacctcaat catgcggtaa gccgcaccat tgctgggctg
     5221 agaggagaaa accgccggaa agcccgctgc tcaaaaagac gccgaaaggc gctgttatct
     5281 gatataggct tgttcatgtc cgcgaattta acatcccatt aacaaaaaac ggggggtagt
     5341 gttgcttctt tgttaactaa catttaatta tttgtttctt ggttagccat tctccagaat
     5401 tatccatcca gcgtcgaata gtccggctgc taacaccaac cgccactgca atatcttccg
     5461 ttg
//
