LOCUS       NZ_CP073364             3904 bp    DNA     circular CON 19-JUN-2023
DEFINITION  Escherichia coli strain EC11 plasmid plas4, complete sequence.
ACCESSION   NZ_CP073364
VERSION     NZ_CP073364.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18818538
            Assembly: GCF_018199975.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Huang,J.
  TITLE     Coexistence of blaNDM-5-IncX3 and mcr-1-IncHI2 plasmids in a ST162
            Escherichia coli strain in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Huang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2021) Department of Clinical Laboratory, Jinhua
            Central Hospital, Mingyue Street 351, Jinhua, Zhejiang 321000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/19/2023 03:17:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,138
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,798
            CDSs (with protein)               :: 4,798
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 85 of 222
            Pseudo Genes (incomplete)         :: 148 of 222
            Pseudo Genes (internal stop)      :: 47 of 222
            Pseudo Genes (multiple problems)  :: 54 of 222
            Pseudo Genes (short protein)      :: 2 of 222
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073364.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC11"
                     /isolation_source="urine"
                     /db_xref="taxon:562"
                     /plasmid="plas4"
                     /geo_loc_name="China: Jinhua"
                     /collection_date="2019-07-25"
     gene            complement(join(3667..3904,1..1313))
                     /locus_tag="KDN44_RS25425"
     CDS             complement(join(3667..3904,1..1313))
                     /locus_tag="KDN44_RS25425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_032495511.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSGN
                     MPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGDRH
                     AYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKKASI
                     PQTAGERKAALVALRSRWADVQNEHLARYGHESRVDHRSLKEQGINRTPEVHLGPVQAA
                     SLNGERIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPELLLQYR
                     KVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARYLDKLGR
                     NVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHERNEFRNKL
                     QQAESEWDKENAAFKRTEGYKYVGDLDRYREREILAAASLENTRQKVAEEVSVARSQML
                     SLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALRIQRSWAREASRSNERDQDMER"
                     /name="MobA/MobL family protein"
     gene            1485..1931
                     /locus_tag="KDN44_RS25430"
     CDS             1485..1931
                     /locus_tag="KDN44_RS25430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_000184723.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKTL
                     SALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVDSK
                     TGKPKFSAAELVGALAGIAELPRNHPKWQEWERRGKELLTKDSA"
                     /name="conjugal transfer protein TraD"
     gene            complement(2192..3211)
                     /locus_tag="KDN44_RS25435"
     CDS             complement(2192..3211)
                     /locus_tag="KDN44_RS25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_011017196.1"
                     /translation="MSAALQYFEENLPRRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKF"
                     /name="replication initiation protein"
     Typing          complement(2437..3211)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.613"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(4..1313)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.077"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gatgccgcgg ccagaatctc acgttctctg tacctgtcca gatccccaac gtacttatat
       61 ccctcggtac gcttaaaagc agcgttttct ttgtcccact cggattcggc ttgctgtagc
      121 ttgttcctga attcattcct ttcatgttca gcggtttcca gcacactttt ttgctccaga
      181 atacgcgcat caatctccct tttgtcagcc ccccgaaaca ggccacgaat cccattcaaa
      241 gcattgcgtt gttcctgtag ctcccgcagc tccctttgtg cggaaacgac attacgcccc
      301 agtttgtcta aataccgtgc cccttcatat attcgcctgc tccattcgga aagagagtct
      361 ttttttgctt tcgcattctg aagcagctta tgttctttga gcgcattggg attggcatcc
      421 cctaaacgag caagccttgc ttctccctga atgaccgttt tcataacctt gcggtactga
      481 agcaataact ccggtgaacg ggctgaacga acaggctcaa ctgccttgat tttttgcttt
      541 tgctcctgtt cctgctgaat ggcatttgcg gcatctctgg cggtcttgag ttcccgctcg
      601 gcattcctcc gttcctggat tgcaacaatc cgctcaccat tgagactggc ggcctgaacg
      661 gggccaagat gaacttccgg cgtgcgattg ataccctgtt ctttcagaga acgatgatct
      721 acgcgcgact cgtgaccgta tcgcgcaagg tgctcattct gcacatctgc ccagcgggaa
      781 cgcagcgcaa caagggcggc ttttcgctct ccggctgtct ggggaatact cgcttttttt
      841 gcgcctcccc gttctggtga tttgctgttg gcacgtttaa aaaattgctg cggatcgcgt
      901 tcaataccat cattaatccg ctcgcaaaac atgatatgcg cgtgtggctg ttcctttccg
      961 tcgattgcac ctggcggatt gtggatcgcg tactggtagg catggcgatc gccaagttcc
     1021 ttttgcacga aatcttcaac aagttctatc cgttgttcgc gtgtcagttc acgcggtaac
     1081 gcgatctcaa tttcacggta tgcagtcccg ttttttcgtt caaattcatc tgcggctttc
     1141 catagctctg cgggattttt cttcgcccat gatggcatgt tacctgatga cgaaaattcc
     1201 agatcctccc gcgatttgta gctcttatat tcgccggatc tggaaatgta atcagcatgt
     1261 tttaaggcac tgccttttgc tccagctttg acggtgcaat ggtagcttgc catctgcctt
     1321 ttagccctcc gcaggagcgc acagtttctc gacgaaggag agaaactact aagtgcgcat
     1381 ccctgaatta atcagggatg attttacggt ctcacacgca ggcagacaag gtgatattat
     1441 gcacaataac aactcacatt aacggcaaaa atgcagaggt aagaatgaca aatcgtgaag
     1501 agtggttaag tgcaaaaatt gcctacatca acggactgaa atcaccgtca gagcagcaga
     1561 gattacttgt gttactggca gagaaaaaaa accgaacaac gacggatgaa aaaacgttgt
     1621 ccgcactgat acgggcagaa aaaaccgcag agaaagcagc cgcagcaaaa gccagggtaa
     1681 cggcaatcat agcggcagaa cggaaggcag ccgcaagggc ggaacgaaag gcgcgggatc
     1741 atgaactcta caaggccgca ggcctcatga ttgtggcagg actggtggac agcaaaaccg
     1801 gaaaaccaaa gtttagtgct gccgaactgg taggcgcact cgcaggaatc gcagagctac
     1861 cgcgcaacca cccaaaatgg caggaatggg aaagacgcgg gaaggagcta ctaaccaaag
     1921 acagtgcgta agagcctccg ttcacgttcc gtttccctgg tgcttcctcg ctcattcgct
     1981 cgctacgctc ggtcatgaga ctgcggcgag cacaaccacc tcaatcatgc ggtaagccgc
     2041 accattgctg ggctgacagg agaaaaccgc cggaaagccc gctgctcaaa aagacgccga
     2101 aaggcgctgt tatctgatat aggcttgttc atgtccgcga atttaaccac ccattaacaa
     2161 aaacaggggg taattgtcat ttttctgtta attaaaattt aattatttgt ttcttggtta
     2221 accattctcc agaattatcc atccagcgtc gaatagtccg gctgctaaca ccaaccgcca
     2281 ctgcaatatc ttccgttgac gctcccaaga tatacatatc aagcgcccta cgacgtttgt
     2341 cgtcgttagc agtccaccga acagcggcgc tttttttccc tttgtccgac tgattttctg
     2401 acgaattggc cttcccgccc ttgcgaccgc gtgctgcctg aatttctggc gtgtgcgtat
     2461 cagccacata ctgtgcgaaa gtttccggcg tgaagttcct gtgcgtgtat ttcgcaatgc
     2521 tcttgccaat agcccgacat tcaggcggag aaagcggaac gggaagcgat gcgttgtaca
     2581 tttcgacacg ctgaatcacg gcatcaagcc actgtgagaa tgctggccag ccctgacgaa
     2641 tcgccctgta agcccatttg cgcgtcattt cgaacaggtg gcagtttcgc cccatcccgt
     2701 aatgtttatc gatgctacga cgcgctgagg cgctcaaatc gagataatca gccagttcat
     2761 cgagggtata ggcttcctcg cgccactcca tcacctgcca ttccaggtgg aacggatttt
     2821 tgcaaatcag tccgctgtag ttcacatcag cacaaagttt ctcacacagc gaacgttcaa
     2881 ccgctgcggc atatttcagc gccttaaccg acgcatcagg cgcggttctc acggcgatat
     2941 tgagtgcata gagcaggtga gcgtgtccgt tcacaggatt tttaacggtg atgttcggcg
     3001 cgggggcgtt ccggtcgctc cagtcaatcg ctgccccctc gcggtcaacg tcaaaaacca
     3061 gccagaactg cgcatgaggc tggttctgct ggatgtaccg cgcaagcagc gcacgccctt
     3121 tgccggagat gcgaagacca aaagcgagat catccgtgtg atagggacgg cggggtaaat
     3181 tttcttcgaa gtattgaagc gcggcgctca tgtgttacga ttccttccag gttatcgacc
     3241 gacatcgccc ctgtattttc gtgacagtct tggcggatag acacgaattt caggggcgtt
     3301 tgtttttcag tcgcttacac tacccccaac gacaaaacct gtcgagtctt cccgcaaacc
     3361 cgatccattt accataactc ctgttgtacg taccccgtaa aacgccctca gtcgcttcct
     3421 gacgcaaaaa acgcaaaaag acaggaaaac accaggccaa accagaaaaa cgcgtcagaa
     3481 tcgttttcag agcgttgcat aactatgcac gaaagcgatg cgcgatctgt ttcgagcgtt
     3541 cagaagggcg aacgggtcaa aacagcaaac tttctcatca gaaaacggtg aacgggacgc
     3601 ggaatttttt cgcgttccgt gaaacgttgg cggggttcgg ggcgcagtcc cgatattgct
     3661 acattttcaa cgctccatat cttgatcacg ttcgttacta cgtgatgcct ccctggccca
     3721 ggatcgctgt atacgcagtg ccttttcctc ctgttgctgc cgttcctgcg ccatttctgc
     3781 cagtaactca tgtcgcattt gagctttttc atctccagat atactgagtt caggctccag
     3841 agataacatc tggctacggg caacggatac ttcttcagca accttttgcc tggtattctc
     3901 taaa
//
