LOCUS       NZ_CP073354            10064 bp    DNA     circular CON 08-DEC-2023
DEFINITION  Klebsiella pneumoniae strain CP19 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP073354
VERSION     NZ_CP073354.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18719795
            Assembly: GCF_021464465.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10064)
  AUTHORS   Wang,J.-T.
  TITLE     The role of transporter and fosA involved in fosfomycin resistance
            in carbapenem resistance Klebsiella pnuemoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10064)
  AUTHORS   Wang,J.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2021) Department of Microbiology, National Taiwan
            University College of Medicine, Ren'ai Road, Taipei 100, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. a3bd8dc
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 54.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/08/2023 11:02:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,561
            CDSs (total)                      :: 5,438
            Genes (coding)                    :: 5,323
            CDSs (with protein)               :: 5,323
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 40 of 115
            Pseudo Genes (incomplete)         :: 73 of 115
            Pseudo Genes (internal stop)      :: 13 of 115
            Pseudo Genes (multiple problems)  :: 11 of 115
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073354.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10064
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CP19"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="Taiwan"
                     /collection_date="2017-10"
     gene            1..372
                     /gene="tolA"
                     /locus_tag="KBT94_RS27660"
     CDS             1..372
                     /gene="tolA"
                     /locus_tag="KBT94_RS27660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            369..734
                     /locus_tag="KBT94_RS27665"
     CDS             369..734
                     /locus_tag="KBT94_RS27665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            909..1187
                     /locus_tag="KBT94_RS27670"
     CDS             909..1187
                     /locus_tag="KBT94_RS27670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            1175..1489
                     /locus_tag="KBT94_RS27675"
     CDS             1175..1489
                     /locus_tag="KBT94_RS27675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1653..2081
                     /locus_tag="KBT94_RS27680"
     CDS             1653..2081
                     /locus_tag="KBT94_RS27680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            2107..2286
                     /locus_tag="KBT94_RS27685"
     CDS             2107..2286
                     /locus_tag="KBT94_RS27685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2313..2843)
                     /locus_tag="KBT94_RS27690"
     CDS             complement(2313..2843)
                     /locus_tag="KBT94_RS27690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2850..3581)
                     /gene="mobC"
                     /locus_tag="KBT94_RS27695"
     CDS             complement(2850..3581)
                     /gene="mobC"
                     /locus_tag="KBT94_RS27695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(3581..5308)
                     /locus_tag="KBT94_RS27700"
     CDS             complement(3581..5308)
                     /locus_tag="KBT94_RS27700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_234696119.1"
                     /translation="MPFSVLASLDSPGRMVRARRHVWNYAYWLTGAAGLALLGGLVVAV
                     FIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLVAGVLLARLLAFLLLDGHFAA
                     ADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNALADFSTEQARKQQAVFLGKD
                     EQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRCVRDFGDAVVYFDPKGDAWAP
                     HVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLVAGFNLSETGDVADHYRISEQ
                     KAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLITKLENVADLSVLQTDSGIDVA
                     GIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARDEFRRWPHASIMLDEIKYLLS
                     KYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFVKTTVLDNTPIRWFYRAASQE
                     SAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQKEARPLFDVNTLQHLPDGFAV
                     MTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEYLPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(7033..7182)
                     /locus_tag="KBT94_RS27705"
     CDS             complement(7033..7182)
                     /locus_tag="KBT94_RS27705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(7267..7524)
                     /locus_tag="KBT94_RS27710"
     CDS             complement(7267..7524)
                     /locus_tag="KBT94_RS27710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(7534..9219)
                     /locus_tag="KBT94_RS27715"
     CDS             complement(7534..9219)
                     /locus_tag="KBT94_RS27715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            9546..9791
                     /locus_tag="KBT94_RS27720"
     CDS             9546..9791
                     /locus_tag="KBT94_RS27720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             2534..10064
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          6352..6930
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2854..3581)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 atgcgtttaa taccctttat cctgctggca ttgtgcagcc ccttactcct gacggcctgt
       61 aagcattcca ccacccagcc gccgaaaatt tatactctgg ataagtcatc taaaatcagt
      121 agctacgctg cggccatcaa gaccgctgtt gaaatgcaga tgtttgaacc agaggcctat
      181 gcgggaaaaa tgtgcaccgt gcggatatcc atacaacgtg atggcactct gaacagcgcc
      241 attgctgaag gaggagatcc gaaactctgt aaagcagcaa tatcagccgt tacccacgcc
      301 aaaattccgc cggcaccgga tgaaaatacc tggcaaatat tcaaaaacgc ccctctggat
      361 ttcagaccat gaaagctttt actctgacag tgctggcggc catgttcctc actccggtac
      421 atgcgatacc actaaaaccc gtaaccacac tgcagccaac ctacccggta caggccgcag
      481 caaaacgcat cgaaggtcag gtgaccgcca cgtttgatat tctggacgat ggacgagtgg
      541 acaacgtgtc gcttaccggc tgtcaccttt tttttcggga aacgctgacg gcaatgaggc
      601 actggctata tcccgcaggg cacccggcaa tgcgacaggt tataaatttt gaattcagtc
      661 tgaaaaatga cggcttacag gatccgcgac cagtcaccac ccaaaagctc aatgatgatg
      721 gttgccgcca gtaacaggtc ctttaacccg caggctgcaa tagtcctgga tgatgagtga
      781 gcctgtagca gccctgatta gtggaagcga agctgaaaaa tgggcagcaa aagccgtatt
      841 tctggcaaat agcgccgcca cggattaaag tagtcatgtg taatacatat gactacaaag
      901 gggcttttat gtcatcagca acatccatca ggctcgatga agaacttaag gaccggctga
      961 aaacgctggc cgatgaccgg caccgctcag cgcacgcgct aatgctggag gcaatcaccg
     1021 aatacatcga ccgggaagag aagcgcagcc agtacctgcg ggacggacag gccgcatggc
     1081 agcactatca ggaaacgggt ctgcacctta ctgcggaaga agcggaagcc tggataagta
     1141 cctggggaac tgagaacgag caggatgctc cgccatgcca caggtaacga tttccgcgct
     1201 ggcacagcgc gatttacaac gccttcagga ctttctcaaa accaaaaatc ggctggcagc
     1261 cagaaaagga ggtgaggtga tcgtccgggc tatccagcaa ctgaaaacat tgccagacat
     1321 tggccgcccg gtgccgtttc tgccgctgga atataaggaa ctggtgatcg ggtttggcga
     1381 cagtggctat gtgatgctct accgccacga ccgggaaatg gaccagattg tgatcgtcac
     1441 cgtcaggcat cagaaagaat ccgggtatcc gggggcagac agcctctaat aaccgatgac
     1501 gggggcgcaa ttcattgcgt ggccgtcgcc ggtttgacaa gcgcagcgcg tcagaaaacc
     1561 acatcctgaa cggaaaaacg ggtaaaattc agcgatacat ttaccgtgag aaattacaca
     1621 cctttcacac ccgataaaat caggggtata cagtggcgaa aacattgctc aacctttcac
     1681 aggcagcaca agccgcgggc attaccagaa gaacgctata caatcatgta aaacagggaa
     1741 aggttaccgt gtcacgtgac gggaaaaaca accctgtcgt ggatgtgtca gaactgatcc
     1801 gtgtatacgg aaatgtgaat atccctgaga aacaaattcc cggcatttca caccgtgaaa
     1861 atacacaaaa aaatttccca caggaacagc ttctggcaat gcaaaaggaa ctggccgatc
     1921 tccggcaggc tgtcaccctg atgctggaag ataaaacatc ccgggaagaa gaacgccgcc
     1981 agcacgatga tgagcgccgg aaactccagg ccgaagtgga cagattgaca acagaactga
     2041 cacagaagaa aaaacgcttc tggtccggct ggtttagctg agaacttacc gaagggaatg
     2101 gagaacatga cgaagcagga aaaaactgcc ctcaatatgg ctaaattcat taaggatcag
     2161 tcgctgctgt tgctggaaaa gctgaacgag ctggacatgg ataccccggc cgacatgtgc
     2221 gaacggcttc acgaggatgc ggaaacactc tggcaggtga tgcgggataa gctcggggaa
     2281 gaataacagc accggccatg ccggtgctgg cgttattttt tgccgaacgg ccaccaccgg
     2341 cgggcgggtt tgtcctccgg cagttcaggc accggcacgt taaaatagcg gctgttatat
     2401 tcccgcatca gctccgcttt cagcttctcg ttcaccgcgc tttccccgtg ccgctcgctg
     2461 accacctcaa tcatcgcgtt aatctgctcc ggtgagcact tcccggcatc aaccacctgc
     2521 acaccgttct cgatggcgaa ccgctccacc tttcgataga ggtaatcaca cagcttcatt
     2581 cctgtgggtt tgtcccgttc ccattgcgga ttttcaccgg tgaaaaagta cgtggctttt
     2641 tcgtcgctgt tgagccgctc gtcatacatc ccgatggatt tcatcatcgc cagatgctgg
     2701 gcccggtcga caaaacagaa aacatacgcc ccggtctgcc aggcgagcac caccatgtct
     2761 tccagatgct cgctggttgg cataatgcag cgctcgccgt ctttgtcttc cacgtcctca
     2821 ataaatacca tgtacagcgc cataatccct cactccgttg tcataaattt aaagcacgcg
     2881 aacagcctct cccggtccag ctccaccgtt ttgcgggcct cgccggtctg tggattcacg
     2941 atttcatcca ccgccagaat cgcccgctgt acccggacaa gcgtcggccg gtcagggcag
     3001 atgtaccaga tttcatcgat gccgttcgcc cggcggttga acagataccc ggacaccact
     3061 tcctgatacc gcctgcggct cttgacggtg cgctctacct caaatgccac cgttctgccg
     3121 tccagctccg ccagcgcgtc cggcactttc atccctttca gggccatacg gtgcaggtac
     3181 cgccagccgg tggccccccg gccctccatg gagaggcgca gggactgcac ggccagccgg
     3241 tgcggcagct gcgccgccga cacccttgac ggctgaaacg gaataacgtc cgtcagcggc
     3301 tcgtcctcgt cgaacgagaa cgccaccccg tgcggcgtca gcccccacac gctgacctga
     3361 cggccgtcaa ccgggtattt cgcccggacc agcagccctt tcgccaccag actgtccagg
     3421 aagcggtaaa ccggctgtac cgccgcaatc cccagccgtc gcgcaaccag ctccgccgtg
     3481 gaacacagct cttcccgcag ccagcgcagg gtcagccgtg ttttctccgc gttccgcgcc
     3541 tgccgcttcg cgtggctgac gttgtactgc tccagtgtca tcagtaaagc cccgcaaaat
     3601 catcatccgc ttttggtccg ccgccgtcgg ccggaggcag gtactccgcc agcgggtccg
     3661 ttttcgccag caccgggaag gttttcagcg aaatttcccg gcgctcaacc cgcagcgggc
     3721 tgctgaacgc cagccgcgcc acgcccagcc cggtcatcac cgcgaacccg tcaggcaggt
     3781 gttgcagggt gttcacgtca aacagcggcc gcgcctcctt ctggatgaag ctgtcgccgc
     3841 tgatgtgctc cacgttcccc gcctcccggc tggcccggcg gcgctccacg tccacccgga
     3901 tctccccggt ctggcccgcc gcccactgcg cggactcctg gctggccgcg cgatagaacc
     3961 agcggatcgg cgtgttatcc agtaccgtgg ttttcacaaa atcagcgggc agatcctgcc
     4021 cgcactgacc gaagtcgccc agcgactggt gcgcgagcag caggttgcag tcccggctgc
     4081 gcagcgtccc cagcgcgttc agcacgtact tcgacagcag atatttaatc tcatcgagca
     4141 tgatgctcgc gtgcggccac ctccggaact catcccgcgc aatgatgatt tgcgcgcagc
     4201 gggcaaacag catcttctgc acccggatca ccgcctcatc gtccatcgag ccgatgacgt
     4261 acaggcagcc gccgccgttg attatccccg ccacatcgat accgctgtcc gtctgcagga
     4321 cgctcaggtc ggccacgttc tccagcttgg tgatcagccc cttcacgtcc tttttcaggg
     4381 cctccggcaa cgcgtacgcc gccgccagca cctgctgaat gtttgccccc tgcgggaact
     4441 gctccgcaat cagcttcgcc gctttctgct cgctgatgcg gtagtggtcc gccacgtcgc
     4501 cggtctcact caggttaaac ccggcaacca gcagattctt cagggcgtac tggtcgagat
     4561 cgcggaacag attcagctgc gccggtttcc ccggccgcag atccagcagg gtgaagtccg
     4621 ggcagtgcgc ccggaagacg tgcggcgccc acgcgtcccc tttggggtcg aagtacacca
     4681 cagcgtcccc gaaatcgcgt acgcagcgaa tgagcgcgtt ggtggccatc gcgctcttgc
     4741 cgctcccggg caggccgagg atctgaatat tggtcttgcg ccaggtatca cgagggacca
     4801 gcaccggctg gccctgctca tccttaccca gaaacaccgc ctgctgtttc cgggcctgct
     4861 ccgtggaaaa atcggccagc gcgttaaccg gtaccggctc cccgaaatgc aggtggcgga
     4921 cgtctgtcat ttctccggtc cgctggctgc tgcgccggct ctcctgattc agtttcgcgt
     4981 tcaccgcctc gtcagcggcc gcaaaatgac catcaagcag caggaacgcc agcagccgcg
     5041 ccagaaggac gccggcgaca agccctccgg cacaccaggc tatcccgaac aggttagccg
     5101 gctgcagcag gtaataatct gcgttcgcat agaggtccgg gcgcaggacg gtttctcccc
     5161 agctctcgat aaacacggcg acaaccagcc cccccagaag ggccagaccg gcagcgccgg
     5221 tcagccagta cgcgtagttc cagacatgtc gccgtgccct gaccatccgc ccgggtgaat
     5281 ccagcgaggc cagcaccgaa aatggcacag tccggttatt atcgcgctta tagcgatcgg
     5341 catactcccc gccgcgcctt atggctcagc cccaccaaaa cgcattcggc cggcccagag
     5401 ttcagccaga ccgaaaaaca ggccgataac cagaaaaaaa accggccaga ctggtaatac
     5461 tcccggtaca gccagctgac ccacgggtaa atctgccggg ccagctccgg gatatgccac
     5521 tgccaggcaa gcgaatccgt attaaacatg caatctctct ccttcagacg acgggtttac
     5581 cggcgtgatg cgcagccccc gctgcacaag ccgcggacat gccccaaggc atggcggcgg
     5641 cggtcacgtc cgtggtgatt gcgttcagct ttcaccgcgg aggccagcct ctttccgccc
     5701 catgcggaaa aggcgcagcc ccggaggaac aacgagcacg ccccagcgtg tgagaggaaa
     5761 aggcggcacc ccagtgcggt cttttccggt gttccggccc ccgtcagagt tcgccatagc
     5821 gagcggctga ccacccgcaa aatcaggagc agctttgctg cttcgtcagg tgcgtccagc
     5881 accgtggatt tttgtgggtg gtcaggcgtg agcctgacgg gggccgggtg aatttctggc
     5941 gtggccggcg tccagccgga cagagccaga aagaggggaa aagaccgtgc gtcggtggcg
     6001 tccagccagc gtaagcaagg tcttttcccc tgaagtcagc cacgccccag cggggatgac
     6061 ttgcatttct ccgcaaggag aaaaaagcaa cagagatagg ttaacgtaaa atgttcagcg
     6121 aaaaaccgac atggttcacc tatcctgata attgatcgtc agggaataga aagacttaat
     6181 caggggacaa tatcccacat cagcgcgtcc ggtgaccgtc ttgcaggtgc cggggaaatt
     6241 gcgcctcctc gctcactcgg tcgctacgct ccgggcgtga gactgcggcg ggcgctgcgg
     6301 acacatacaa agttacccac agattccgtg gataagcagg ggactaacat gtgaggcaaa
     6361 acggcagggc cgcgccggtg gcgtttttcc ataggctccg ccctcctgcc agagttcaca
     6421 taaacagacg ctttttcggt gcatctgtgg gagccgtgag gctcaaccat gaatctgaca
     6481 gtacgggcga aacccgacag gacttaaaga tccccaccgt ttccggtagg tcgctccctc
     6541 ttgcgctctc ctgttccgac cctgccgttt accggatacc tgtccgccgt tctcccttgc
     6601 gggaagtgtg gcgctttctc atagctcaca cactggtatc tcggctcggt gtaggtcgtt
     6661 cgctccaagc tgggctgtaa gcaagaactc cccgttcagc ccgactgctg cgccttatcc
     6721 ggtaactgtt cacttgagtc caacccggga aagcacgcaa aacgccactg gcagcagcca
     6781 ttggtaactg ggagttcgca gaggatttgt ttagctaaac acgcggttgc tcttgaagtg
     6841 tgcgccaaag tccggctaca ctggaaggac agatttggtt gctgtgctct gcgaaagcca
     6901 gttaccacgg ttaagcagtt ccccaactga cttaaccttc gatcaaacca cctccccagg
     6961 tggttttttc gtttacaggg caaaagatta cgcgcagaaa aaaaggatct caagaagatc
     7021 ctttgatctt ttctactgaa ccgcgatccc cgtcagttca gaggaggacg atggtgccac
     7081 cgttccgccc tgaacatccc ggatataatt tgcctgacat gcggccagaa gaaagccagc
     7141 aacaatcaat ataaaaagaa aaatagtctt tacctttttc atatactttc cctgtattta
     7201 aagactccgg cattcatccc tgaacaccgg aagtcagtat ttcaaatcat ccttgataat
     7261 tttttgttac cagttaccgt cccgataatc aaaagctata taacaattat ccacatcagg
     7321 ttttatttga tttttaaaaa atggctgtag taaaggaacc cattctttct tcaaatcaaa
     7381 ctcaccatta ttaatattat cctttaaagg cattcccaag ctttcaatga cagaaccatc
     7441 gtcaccaaag tctttagaat actcttcccc tttaaactct tcagttttct tatcaaacca
     7501 tgtcaaacgt aatttaagcc ccatatttac ctctcaaaga tattttttaa tgttccgttt
     7561 tggatctggc cctttaacct gcttacccgt tttgggatca aatgcgccta ggtgttcgcc
     7621 atcactggca cgataccctt caagctcacc gtgctgggaa tcccattcat aaattttacg
     7681 ttttttatcg ccataccatc gggcccgctt accaccacca ccttgtttag gtgttttagg
     7741 atcacctcgc tttaactctc caagtccctt tatgtcttct gttttaggta ctggatgata
     7801 atcatggccg tagtctttag tccccttgcg aggcttgttc ttttcctcat ttaacttatt
     7861 ttctgcatcc ttcgcttttt tctccttttg tttccggctc tccatcgccc cggtaagtgc
     7921 agccttatct ctggataagg attcattcat tgcgtttaaa gatgcctgag ccgcttttaa
     7981 tttctcattc tgattctgaa tatccttatc aagatagcga acctgatcct gccacccgga
     8041 gccatattca tgaggcgggt tataagtcac aaaatcatct ttatttgcct ctgcctcttt
     8101 taccttttta tcggcttcct gaacagcagc ccttgcaccg gggagcgcct tctctgtcga
     8161 tgcaatgcgg ctgtttaagg tcgcaatatt tttatcttcg gcatccagct cagctttcgc
     8221 tttatcatag tctcttttca gcccctcttc cggatgagcg gcgtcccatg cctgctggcg
     8281 acgcttttct tcctcctgac gctgttttac ctgtgccggg gtaagaacat ctgtcaccga
     8341 cacataaacc ggcttctgtc cggtctcttt cgggaaacga ataacggcct cacgggagtt
     8401 accaccggct gtaaaaccag ccggacgtga atcacctttt tcagcaacaa tgccttttgg
     8461 cggggctttc gctgtcggaa cacctttcgg tacgctcacc tgcagagacg ggagacccgg
     8521 aatcgacaca ctgaataccc ccggacgttt tgtcggtttc gcatccacca cagggacact
     8581 cattggccga cctgcgacaa ccgcaatatg ctgccgctca tccttcacca catccacaac
     8641 ccgttgtctg acacgaaccg tcgcctggtc cagtggtaaa gtacttgccg gagtctccgt
     8701 caccgcatcg gccggtaatg acgtcataat ttttgacatc attttcggat catcttttgc
     8761 aatctcagaa ggcagcacac cgtagatcgc aatcccccac agaccaaact taaacggccc
     8821 cttcagggca gccagcacat cagcaacggc ggccgacaac gcatcgccgg aaactgataa
     8881 agccagtcct tcagcccccg gagtagccag ggcaggcaga ccgaaggcca tcgcagtggc
     8941 cgaagactgt ccgccaccag agctaccgct atgattgccg ctgttaccac cattaccacc
     9001 gggtttactc ccgccgttac caaactcccc cggattatag ttatgaatat ctccattcgg
     9061 cgtatgcgtc gtaccccagc ccggcccgct gtttggatcc gtaccaccac ctgatgaagg
     9121 gccaccttta ccagacgtac cgttcacatt cccactggcc tgaccaccat tatgtcccag
     9181 acctgaatta cccggacctc gaccgtctcc accactcata ccgttttcct ctttgacatt
     9241 taaaacaata aattaaagcc aacatactgt acatatacac agtataatta ttaccttact
     9301 cctcccgttt tcaggtgtca atatatgaga ttagttctca tcttcaggca caaaaaagcg
     9361 cccggagcca cctgtaagca gatcgcgtct tttcccgaaa atttctcatg gcaacaaatt
     9421 ttattattta agacattcat cacacttaca tttcagatgg tatagacaca tctgaaagct
     9481 aactggcatg aggagaggac ctgcggttaa actgttcggg aaacctgaga cgcccggagg
     9541 ccactatgac gcacctgaaa ctgactcccg ctgaagaaaa gcgcctggcg gacgcctttg
     9601 tcgctcaggc actggccatg ggcctgaaag ggaacaaaac gctgccaaag gcggtaaaac
     9661 aacaggtgcg ggaaagggtg cttcgtgcca ttcaggacga acgtgaagcc aggcgggcgc
     9721 aggaaatgcg tcgggagaaa tacgaggcta cggaaaccac cttcacctgg cagccacagc
     9781 gccgccggta aggggtattt accataacgt cgcattgccc gcaccgctca gacggtctga
     9841 gggcgtttcc ggcggctacg ggataaaaca ggcaacaaac cacccggaaa accgccggaa
     9901 acgcgcccgg taaattgtaa cccgctgcat gcctatgcac gcaactgcat ggcgggccgt
     9961 ttggtgtaaa aacgatacga taagccgtgt ttatgtaaat gaacgtggcc gtaaagagtg
    10021 acaaatatac atgtaacatt ttttactgac aggtcatcaa cccg
//
