LOCUS       NZ_CP073313             7973 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterobacter cloacae strain CZ862 plasmid pCZ862_GES1_GES5, complete
            sequence.
ACCESSION   NZ_CP073313
VERSION     NZ_CP073313.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18753389
            Assembly: GCF_018140965.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 7973)
  AUTHORS   Finianos,M., Kraftova,L., Papagiannitsis,C.C., Adamkova,V.,
            Hrabak,J. and Bitar,I.
  TITLE     Genomic characterisation of three GES-producing Enterobacterales
            isolated in the Czech Republic
  JOURNAL   J Glob Antimicrob Resist 29, 116-119 (2022)
   PUBMED   35231657
REFERENCE   2  (bases 1 to 7973)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial assembly v. 1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 670X
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018140965.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 04:38:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,479
            CDSs (total)                      :: 5,360
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 53 of 288
            Pseudo Genes (incomplete)         :: 204 of 288
            Pseudo Genes (internal stop)      :: 51 of 288
            Pseudo Genes (multiple problems)  :: 20 of 288
            Pseudo Genes (short protein)      :: 1 of 288
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073313.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7973
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="CZ862"
                     /isolation_source="Hospital environment"
                     /db_xref="taxon:550"
                     /plasmid="pCZ862_GES1_GES5"
                     /geo_loc_name="Czech Republic: Prague"
                     /lat_lon="50.07 N 14.43 E"
                     /collection_date="2019-12-05"
                     /collected_by="VFU"
     gene            complement(join(6700..7973,1..181))
                     /locus_tag="KDU74_RS27445"
     CDS             complement(join(6700..7973,1..181))
                     /locus_tag="KDU74_RS27445"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_233471716.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGSPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRANKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASERRVHEARESYLRGVEIKRGENERRY
                     CRAQPEYGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASQSPSLGGEGRQDKA
                     GPVREEVGAPLRADLRFQPGRLGEGYVLGGSEGVLNDGIGAGIVLRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQARGREQPGSGETLDVASGRLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKVLDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(162..500)
                     /locus_tag="KDU74_RS26850"
                     /old_locus_tag="KDU74_26850"
     CDS             complement(162..500)
                     /locus_tag="KDU74_RS26850"
                     /old_locus_tag="KDU74_26850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005322038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_160387705.1"
                     /translation="MAGQPLKRIRSIKIRVSDAELERLREICPKAQLAEWMREQCLGVV
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(482..700)
                     /locus_tag="KDU74_RS26855"
                     /old_locus_tag="KDU74_26855"
     CDS             complement(482..700)
                     /locus_tag="KDU74_RS26855"
                     /old_locus_tag="KDU74_26855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_160387704.1"
                     /translation="MPTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEYCNFR
                     DEKALQTLLDSLYEDALAEATAWLASR"
                     /name="hypothetical protein"
     gene            complement(1360..2373)
                     /gene="intI1"
                     /locus_tag="KDU74_RS26860"
                     /old_locus_tag="KDU74_26860"
     CDS             complement(1360..2373)
                     /gene="intI1"
                     /locus_tag="KDU74_RS26860"
                     /old_locus_tag="KDU74_26860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="WP_211944504.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRESIRYLHYSLQTEQAYVHWVRAFI
                     RFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQEI
                     GRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDFDHG
                     TIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALERKYPR
                     AGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATPHTLRH
                     SFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLTSER"
                     /name="intI1"
     gene            2553..3416
                     /locus_tag="KDU74_RS26865"
                     /old_locus_tag="KDU74_26865"
     CDS             2553..3416
                     /locus_tag="KDU74_RS26865"
                     /old_locus_tag="KDU74_26865"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class A beta-lactamase
                     GES-5"
                     /protein_id="WP_012658785.1"
                     /translation="MRFIHALLLAGIAHSAYASEKLTFKTDLEKLEREKAAQIGVAIVD
                     PQGEIVAGHRMAQRFAMCSTFKFPLAALVFERIDSGTERGDRKLSYGPDMIVEWSPATE
                     RFLASGHMTVLEAAQAAVQLSDNGATNLLLREIGGPAAMTQYFRKIGDSVSRLDRKEPE
                     MSDNTPGDLRDTTTPIAMARTVAKVLYGGALTSTSTHTIERWLIGNQTGDATLRAGFPK
                     DWVVGEKTGTCANGGRNDIGFFKAQERDYAVAVYTTAPKLSAVERDELVASVGQVITQL
                     ILSTDK"
                     /name="carbapenem-hydrolyzing class A beta-lactamase GES-5"
     gene            3573..4436
                     /locus_tag="KDU74_RS26870"
                     /old_locus_tag="KDU74_26870"
     CDS             3573..4436
                     /locus_tag="KDU74_RS26870"
                     /old_locus_tag="KDU74_26870"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase GES-1"
                     /protein_id="WP_013250881.1"
                     /translation="MRFIHALLLAGIAHSAYASEKLTFKTDLEKLEREKAAQIGVAIVD
                     PQGEIVAGHRMAQRFAMCSTFKFPLAALVFERIDSGTERGDRKLSYGPDMIVEWSPATE
                     RFLASGHMTVLEAAQAAVQLSDNGATNLLLREIGGPAAMTQYFRKIGDSVSRLDRKEPE
                     MGDNTPGDLRDTTTPIAMARTVAKVLYGGALTSTSTHTIERWLIGNQTGDATLRAGFPK
                     DWVVGEKTGTCANGGRNDIGFFKAQERDYAVAVYTTAPKLSAVERDELVASVGQVITQL
                     ILSTDK"
                     /name="extended-spectrum class A beta-lactamase GES-1"
     gene            4559..5350
                     /locus_tag="KDU74_RS26875"
                     /old_locus_tag="KDU74_26875"
     CDS             4559..5350
                     /locus_tag="KDU74_RS26875"
                     /old_locus_tag="KDU74_26875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="WP_172692819.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSQPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPAQYQP
                     VLLEAKQAYLGQKEDHLASRADHLEEFIRFVKGEIIKSVGK"
                     /name="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
     gene            complement(5377..>5442)
                     /locus_tag="KDU74_RS26880"
                     /old_locus_tag="KDU74_26880"
                     /pseudo=""
     CDS             complement(5377..>5442)
                     /locus_tag="KDU74_RS26880"
                     /old_locus_tag="KDU74_26880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /name="EAL domain-containing protein"
     gene            complement(5412..5606)
                     /locus_tag="KDU74_RS26885"
                     /old_locus_tag="KDU74_26885"
     CDS             complement(5412..5606)
                     /locus_tag="KDU74_RS26885"
                     /old_locus_tag="KDU74_26885"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172692818.1"
                     /translation="MTLHEIFVGEELEMEQFDLERIADLIYGWLEGEHIDPQMAHYAGM
                     LLLSLSQRALSFSEDDCTS"
                     /name="hypothetical protein"
     gene            complement(5603..6517)
                     /locus_tag="KDU74_RS26890"
                     /old_locus_tag="KDU74_26890"
     CDS             complement(5603..6517)
                     /locus_tag="KDU74_RS26890"
                     /old_locus_tag="KDU74_26890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_160387708.1"
                     /translation="MTNRAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLNWLADYLDLTP
                     YNGRKQLPAYGLGRNCTLFEKTRQWAYKAIRQGWPGYREWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLTPAGFSAWQAEQGAKKGKALRDLMLPVVEELLNQGLAKA
                     DIARELGQSKATIGNWVKRLEIK"
                     /name="replication initiation protein"
     ARG             3574..4433
                     /name="blaGES-1"
                     /gene="blaGES-1"
                     /gene_name="extended-spectrum class A beta-lactamase GES-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             2554..3413
                     /name="blaGES-5"
                     /gene="blaGES-5"
                     /gene_name="carbapenem-hydrolyzing class A beta-lactamase
                     GES-5"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             4560..5347
                     /name="aadA15"
                     /gene="aadA15"
                     /gene_name="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA15"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.62"
                     /coverage="100.0"
     Typing          complement(6704..7971)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.726"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 caaacgacag gacgccagag gtgtattttt tggcgtaggg actggaatca atcagggctt
       61 caatcaccgc agggctgccc cgatcgagcg ttgcgccttc ccggttgccg tcacggccca
      121 acaggtagcc aacaggcccg gagccccggc ctaccccacg gttatggaat ttgatgatca
      181 tggtgcagtt cctccagctc gcggcctata gcggacagct ccgctatgat gcgcagccga
      241 tccaacggcc cccactcccc ggaatttacg cgccgggcga tctggttcag gttgttgccc
      301 attccggcca actgccgcaa gagcgccgga tctacggtag gcgccggcgt tcggcgctgg
      361 ggctgcacaa cgccgagaca ttgctcccgc atccactccg ccagttgggc cttggggcag
      421 atctcccgca ggcgctcaag ctccgcatct gaaacccgga tcttgataga gcggatgcgc
      481 tttaacggct ggccagccat gcggtggcct ccgctaaggc gtcctcatag aggctatcga
      541 gtagcgtctg gagcgccttt tcatcccgaa aattgcagta ttccggcaag gggaaaacac
      601 cccccccgac ctcttcgccg ttctccagca aacgcagatt ccagcccccg ccaagcccga
      661 gagtgcgggg ggtgatttcg taatcgtatt ccgtgggcat cgtccattct ccagagccaa
      721 gaagccgccg cgcagaccgt cccgaagggg cggatcgagc gggggccaca ggggggtttc
      781 gggggggcgt agcacccctg aacaaggcca cagagccccg agaccgcgaa gcaggtagag
      841 ggggctctgt gtagccttgc ttagtacatt attcaactat gggcgggata ctgcaagccc
      901 taggtctaga tcaagggcaa gagctgctcc gtggataact gctgccgccc acaccccccg
      961 aaaaaacagg ggggcgatgg acggacaggc gcagttgtcc acctcgccaa ggatccgcgc
     1021 taaaaggatt ttttacggct aaaagacaag agcgcaggaa ccccctaacg gggctattat
     1081 ctgatatggc ttttttactc ttttttaaaa gacgagtttt ttgttggatt cactgtgcat
     1141 cagggagatc tggcgcttgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     1201 tgcactatag cagcggaggg gttggatcca tcaggcaacg acgggctgct gccggccatc
     1261 agcggacgca gggaggactt tccgcaaccg gccgttcgat gcggcaccga tggccttcgc
     1321 gcaggggtag tgaatccgcc aggattgact tgcgctgccc tacctctcac tagtgagggg
     1381 cggcagcgca tcaagcggtg agcgcactcc ggcaccgcca actttcagca catgcgtgta
     1441 aatcatcgtc gtagagacgt cggaatggcc gagcagatcc tgcacggttc gaatgtcgta
     1501 accgctgcgg agcaaggccg tcgcgaacga gtggcggagg gtgtgcggtg tggcgggctt
     1561 cgtgatgcct gcttgttcta cggcacgttt gaaggcgcgc tgaaaggtct ggtcatacat
     1621 gtgatggcga cgcacgacac cgctccgtgg atcggtcgaa tgcgtgtgct gcgcaaaaac
     1681 ccagaaccac ggccaggaat gcccggcgcg cggatacttc cgctcaaggg cgtcgggaag
     1741 cgcaacgccg ctgcggccct cggcctggtc cttcagccac catgcccgtg cacgcgacag
     1801 ctgctcgcgc aggctgggtg ccaagctctc gggtaacatc aaggcccgat ccttggagcc
     1861 cttgccctcc cgcacgatga tcgtgccgtg atcgaaatcc agatccttga cccgcagttg
     1921 caaaccctca ctgatccgca tgcccgttcc atacagaagc tgggcgaaca aacgatgctc
     1981 gccttccaga aaaccgagga tgcgaaccac ttcatccggg gtcagcacca ccggcaagcg
     2041 ccgcgacggc cgaggtcttc cgatctcctg aagccagggc agatccgtgc acagcacctt
     2101 gccgtagaag aacagcaagg ccgccaatgc ctgacgatgc gtggagaccg aaaccttgcg
     2161 ctcgttcgcc agccaggaca gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg
     2221 acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggtttg
     2281 taaactgtaa tgcaagtagc gtatgctctc acgcaactgg tccagaacct tgaccgaacg
     2341 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttgtaca
     2401 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     2461 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt tagacgggcg
     2521 tacaaagata atttccatct caagggatca ccatgcgctt cattcacgca ctattactgg
     2581 cagggatcgc tcactctgca tatgcgtcgg aaaaattaac cttcaagacc gatcttgaga
     2641 agctagagcg cgaaaaagca gctcagatcg gtgttgcgat cgtcgatccc caaggagaga
     2701 tcgtcgcggg ccaccgaatg gcgcagcgtt ttgcaatgtg ctcaacgttc aagtttccgc
     2761 tagccgcgct ggtctttgaa agaattgact caggcaccga gcggggggat cgaaaacttt
     2821 catatgggcc ggacatgatc gtcgaatggt ctcctgccac ggagcggttt ctagcatcgg
     2881 gacacatgac ggttctcgag gcagcgcaag ctgcggtgca gcttagcgac aatggggcta
     2941 ctaacctctt actgagagaa attggcggac ctgctgcaat gacgcagtat tttcgtaaaa
     3001 ttggcgactc tgtgagtcgg ctagaccgga aagagccgga gatgagcgac aacacacctg
     3061 gcgacctcag agatacaact acgcctattg ctatggcacg tactgtggct aaagtcctct
     3121 atggcggcgc actgacgtcc acctcgaccc acaccattga gaggtggctg atcggaaacc
     3181 aaacgggaga cgcgacacta cgagcgggtt ttcctaaaga ttgggttgtt ggagagaaaa
     3241 ctggtacctg cgccaacggg ggccggaacg acattggttt ttttaaagcc caggagagag
     3301 attacgctgt agcggtgtat acaacggccc cgaaactatc ggccgtagaa cgtgacgaat
     3361 tagttgcctc tgtcggtcaa gttattacac aactcatcct gagcacggac aaatagttga
     3421 cgcccgtcta acaattcgtt caagccgacg ttgcttcgtg gcggcgcttg cgtgctacgc
     3481 taagcttcgc acgccgcttg ccactgcgca ccgcggctta actcaggcgt tagacgggcg
     3541 tacaaagata atttccatct caagggatca ccatgcgctt cattcacgca ctattactgg
     3601 cagggatcgc tcactctgca tatgcgtcgg aaaaattaac cttcaagacc gatcttgaga
     3661 agctagagcg cgaaaaagca gctcagatcg gtgttgcgat cgtcgatccc caaggagaga
     3721 tcgtcgcggg ccaccgaatg gcgcagcgtt ttgcaatgtg ctcaacgttc aagtttccgc
     3781 tagccgcgct ggtctttgaa agaattgact caggcaccga gcggggggat cgaaaacttt
     3841 catatgggcc ggacatgatc gtcgaatggt ctcctgccac ggagcggttt ctagcatcgg
     3901 gacacatgac ggttctcgag gcagcgcaag ctgcggtgca gcttagcgac aatggggcta
     3961 ctaacctctt actgagagaa attggcggac ctgctgcaat gacgcagtat tttcgtaaaa
     4021 ttggcgactc tgtgagtcgg ctagaccgga aagagccgga gatgggcgac aacacacctg
     4081 gcgacctcag agatacaact acgcctattg ctatggcacg tactgtggct aaagtcctct
     4141 atggcggcgc actgacgtcc acctcgaccc acaccattga gaggtggctg atcggaaacc
     4201 aaacgggaga cgcgacacta cgagcgggtt ttcctaaaga ttgggttgtt ggagagaaaa
     4261 ctggtacctg cgccaacggg ggccggaacg acattggttt ttttaaagcc caggagagag
     4321 attacgctgt agcggtgtat acaacggccc cgaaactatc ggccgtagaa cgtgacgaat
     4381 tagttgcctc tgtcggtcaa gttattacac aactcatcct gagcacggac aaatagttga
     4441 cgcccgtcta acaattcgtt caagccgacg ttgcttcgtg gcggcgcttg cgtgctacgc
     4501 taagcttcgc acgccgcttg ccactgcgca ccgcggctta actcaggcgt taaacatcat
     4561 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     4621 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     4681 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     4741 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     4801 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     4861 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     4921 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     4981 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     5041 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgcagcccg actgggccgg
     5101 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcaa taaccggcaa
     5161 aatcgcgccg aaggatgtcg ctgccgactg ggcaataaaa cgcctacctg cccagtatca
     5221 gcccgtctta cttgaagcta agcaagctta tctgggacaa aaagaagatc acttggcctc
     5281 acgcgcagat cacttggaag aatttattcg ctttgtgaaa ggcgagatca tcaagtcagt
     5341 tggtaaatga tgtctaacac ttctgatatt gaactgtcag gagctggctg cacaacagcc
     5401 attacgccca atcaactggt gcagtcgtct tctgaaaatg acagcgctcg ctgtgacaga
     5461 gacaaaagga gcattccagc atagtgcgcc atttgggggt caatgtgctc accctccagc
     5521 cagccgtata tcagatcggc tatacgctct agatcaaact gttccatttc tagttcttca
     5581 ccaacgaaga tttcgtgcag agtcatttga tctccaatct ttttacccaa ttacctatgg
     5641 tcgccttgga ctgtcccagc tctcgtgcaa tatcagcctt ggctaatcct tggttcagca
     5701 gctcctcgac tacgggtaac atcaaatccc ggagcgcctt gcccttcttg gctccctgct
     5761 ctgcttgcca tgcgctaaag cccgcagggg ttaaatgctg gtgcgtccat ttagcaatgg
     5821 acttggccgt gtgccggact tcgtttgcgg ggagcggctg ctcgaactgg gcgttatatc
     5881 ctgatgcccg atcaacacaa gccgccaacc attcgcggta acccggccag ccctgccgga
     5941 tcgctttata agcccactgg cgagttttct cgaatagggt gcagtttcgc cccagtccgt
     6001 aggcaggaag ctgtttgcgg ccattgtagg gcgtcaaatc gaggtagtcg gccagccagt
     6061 tcagatcgta gagccggggc tcccatacct gcaccagcca gtgctcatgc agggggttct
     6121 tgcagatcaa cccggagtag cccatatccg cccctaattt ctcccggagc gcggcctcga
     6181 tggcggcggc ataccgcaga ggggctgcat ttccatccgg ggcagtgcgg atcggcgtat
     6241 ccagtccgta gatcagatgc gcgtggccgt tggccttgtt cgtgacggta atgttagggg
     6301 ctggagcgcc tacgtcatcc caagataacg tagcggcggc atggtctacg tcaaaaacga
     6361 tccagtggcg gtgcgttggc ccgttcggct ggatgtaacg cgcaccaagg gcgcgggcaa
     6421 catccgcaat ccgtaggccc gttgtaagct cgtcggaata gtaaggcttc cggggcaggc
     6481 gctcccggaa cagctcaagc tgtgctctgt tggtcaaaat ccaccgcgct tattggcggt
     6541 ctggggttgt ttggtctgat ccatgtggta tgcttttcct gttcttagtg aacggccccg
     6601 aatgctctga gtcttccgcc aagttgatca gcagagcttc ggggtttgtc ttttctgcac
     6661 agtacgttac accaaaaaac gtcctgatcc tactgggcgc tatcgctcaa agcctcgcga
     6721 tctgtctcca cgctcccagc ctcgatccag taccttcagg agcctgtgtt ccctttcttt
     6781 tgcaacttgc tgactagccc ggtcaagccc tccagctgct tgctcaagcc gtccacttgc
     6841 tgcgttaagc cgtccacttg ccacgtcaag cgtttcgccg cttcccggct gctctcggcc
     6901 tctcgcttgg tgctctcgta catgctcgcc aagctcgcgt agctttcctg ccatgccttg
     6961 tgctgctgct cgtatgcgct ctgcaaagcc tcgaacgcgc agaacaattc ccgctccaat
     7021 cccgtcattt agtacccctt cggaaccacc caaaacgtat ccttccccca aacgtccggg
     7081 ttggaatcgg agatctgcac gcaaaggtgc tccgacttct tcccgcaccg ggccagcttt
     7141 atcttgccgc ccttctccgc caaggcttgg agattggctt gccgctccgc tatcaattcc
     7201 ccctgatacc aaaggatccc gctcgttgcg ctcaagaaca ggacgatgct gaccagcatc
     7261 cagatccacg ttttgagcaa cagccagccc atattctggc tgtgctcggc aatagcggcg
     7321 ctcattctct cctcgcttga tttcaacgcc ccggagatag ctttctcgtg cctcgtgaac
     7381 tcttcgctca gacgcttctc gatagcgttg gcctgctgct tcgagttctg ctcgaagtcc
     7441 ttcgccatgt ctaaaatctt gctcatacag cacccctttt agtcggatgt tccgcccccc
     7501 gtccgggtcg gcaatgctca ggctgttttt ggtttcgcgt accacgctga aacccgcacc
     7561 ctgcaaggct ttgcgcacat catcgcggct ctggatctcc cctgccccag ccatgcgcaa
     7621 aagcccgtct gtaatagcct gagcggcctc cttgtgcccc ctcggcaaat cctttggcgt
     7681 gataacggcc tgacggttta acggatcgtc cgggtcgtgc aatttcagct ccccgtttac
     7741 cacggttcgc cacgcatcga ggcgcggatt gtcggccttg tggaagtagg gttgcagccg
     7801 cttcccgctc tgcaattcga tgttcgggac tacaaaattc agctccagcc gccccttgtc
     7861 gcggtgctct acccacaaaa cagcgtactg gttgccctcc agtccgggca ggagcgcctt
     7921 ttcaaagtcg ctcatcagct tgtccttgtt cgccctgggt aaatcagcct cgg
//
