LOCUS       NZ_CP073312             8282 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterobacter cloacae strain CZ862 plasmid pCZ862_ColRNAI, complete
            sequence.
ACCESSION   NZ_CP073312
VERSION     NZ_CP073312.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18753389
            Assembly: GCF_018140965.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Finianos,M., Kraftova,L., Papagiannitsis,C.C., Adamkova,V.,
            Hrabak,J. and Bitar,I.
  TITLE     Genomic characterisation of three GES-producing Enterobacterales
            isolated in the Czech Republic
  JOURNAL   J Glob Antimicrob Resist 29, 116-119 (2022)
   PUBMED   35231657
REFERENCE   2  (bases 1 to 8282)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial assembly v. 1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 670X
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018140965.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 04:38:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,479
            CDSs (total)                      :: 5,360
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 53 of 288
            Pseudo Genes (incomplete)         :: 204 of 288
            Pseudo Genes (internal stop)      :: 51 of 288
            Pseudo Genes (multiple problems)  :: 20 of 288
            Pseudo Genes (short protein)      :: 1 of 288
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073312.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="CZ862"
                     /isolation_source="Hospital environment"
                     /db_xref="taxon:550"
                     /plasmid="pCZ862_ColRNAI"
                     /geo_loc_name="Czech Republic: Prague"
                     /lat_lon="50.07 N 14.43 E"
                     /collection_date="2019-12-05"
                     /collected_by="VFU"
     gene            join(8205..8282,1..453)
                     /locus_tag="KDU74_RS26790"
                     /old_locus_tag="KDU74_26790"
     CDS             join(8205..8282,1..453)
                     /locus_tag="KDU74_RS26790"
                     /old_locus_tag="KDU74_26790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044704832.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMAVMAWQTGAYVFRFE
                     DQAQHLAMMKFIGMYHERLNSDDKATYYFTGKNPAWERDKPSGMKLCDYLYRKVEQFAI
                     ENGVQVLDAGKYTPEQIGAMIDVVSERHGESAVNEKLKAELMREYNSRYFNEPVPVLPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(481..660)
                     /locus_tag="KDU74_RS26795"
                     /old_locus_tag="KDU74_26795"
     CDS             complement(481..660)
                     /locus_tag="KDU74_RS26795"
                     /old_locus_tag="KDU74_26795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032934837.1"
                     /translation="MTKQEKTALNMAKFIRDQSLLLLEKLNELDMDDPADMCERLHEDA
                     EKLWLVMRGKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            730..1107
                     /locus_tag="KDU74_RS26800"
                     /old_locus_tag="KDU74_26800"
     CDS             730..1107
                     /locus_tag="KDU74_RS26800"
                     /old_locus_tag="KDU74_26800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321415.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQGETHERDGLRLPDETEKTASEALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETVETPAETPRRWWHWGKR"
                     /name="hypothetical protein"
     gene            complement(1169..1468)
                     /locus_tag="KDU74_RS26805"
                     /old_locus_tag="KDU74_26805"
     CDS             complement(1169..1468)
                     /locus_tag="KDU74_RS26805"
                     /old_locus_tag="KDU74_26805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010848978.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_009365129.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYEVDDPAQKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1458..1721)
                     /locus_tag="KDU74_RS26810"
                     /old_locus_tag="KDU74_26810"
     CDS             complement(1458..1721)
                     /locus_tag="KDU74_RS26810"
                     /old_locus_tag="KDU74_26810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            2495..3013
                     /locus_tag="KDU74_RS26815"
                     /old_locus_tag="KDU74_26815"
     CDS             2495..3013
                     /locus_tag="KDU74_RS26815"
                     /old_locus_tag="KDU74_26815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044704828.1"
                     /translation="MQKVTDLYPPEIARHKLQNHFAGNTVMLELIQKLNKTSLCTFAAL
                     CDGNVVTTSGYNITADLCVNRASAVAHSLKQKYLPVTARTISTKADVGGAVKQAAFCID
                     EDDLARLKSEPEKMMKECERNLNSQKRTNAQKEMSRLYKEFGEDGILALLRNVARSNGT
                     PPPGAHPAS"
                     /name="hypothetical protein"
     gene            complement(3044..3298)
                     /locus_tag="KDU74_RS26820"
                     /old_locus_tag="KDU74_26820"
     CDS             complement(3044..3298)
                     /locus_tag="KDU74_RS26820"
                     /old_locus_tag="KDU74_26820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321408.1"
                     /translation="MNFKQLIPAQDWYFRHFTDQSDYPVIVYQLAAWALTEEGSITGLV
                     AIYDPKEGKAKLVTPPPVKGNYLHRDQLTDEELAASRKP"
                     /name="hypothetical protein"
     gene            complement(3311..3562)
                     /locus_tag="KDU74_RS26825"
                     /old_locus_tag="KDU74_26825"
     CDS             complement(3311..3562)
                     /locus_tag="KDU74_RS26825"
                     /old_locus_tag="KDU74_26825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321409.1"
                     /translation="MALVQKKTLNELATNASDDFFGKRFSAGDEVPHSGIYKCTKCNRE
                     VTVNAHSRDNTFPPHYPESPCKSPTWKPYVIADTKAEW"
                     /name="hypothetical protein"
     gene            3716..4048
                     /locus_tag="KDU74_RS26830"
                     /old_locus_tag="KDU74_26830"
     CDS             3716..4048
                     /locus_tag="KDU74_RS26830"
                     /old_locus_tag="KDU74_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321410.1"
                     /translation="MEKQYTNELTAEILAGMDQSPFTPEQLAAMSDEARALIEEQEAFC
                     HAHPVTTIYRLAVAGCLTRRGGTGDEFNPNPEEGHKIRLENGLWVSVLTEGCTVTYPDG
                     TQARIL"
                     /name="hypothetical protein"
     gene            5506..7467
                     /locus_tag="KDU74_RS26835"
                     /old_locus_tag="KDU74_26835"
     CDS             5506..7467
                     /locus_tag="KDU74_RS26835"
                     /old_locus_tag="KDU74_26835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_044704830.1"
                     /translation="MLNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNDRAAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLVLLGGLVVAVFIESWGETVLRPGYYANADYYLLQPANLFGIAWCAGGLV
                     VGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARQQQVVFLGKDEDGQPVRVPREAWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLAETGDVADHYRISEQKAAKLIAEQFPHGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRAWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QAEAVRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            7467..8198
                     /locus_tag="KDU74_RS26840"
                     /old_locus_tag="KDU74_26840"
     CDS             7467..8198
                     /locus_tag="KDU74_RS26840"
                     /old_locus_tag="KDU74_26840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="WP_008321412.1"
                     /translation="MALEQYNVSHAKRQARNAEKTRLVLRWLRDELCSTAETVARLLGI
                     GAVQPVYRFLDSLVAKGLLARAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLDMEARGATGWRTLHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     Typing          4324..4512
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.529"
                     /coverage="94.0"
                     /evalue="1.1199999999999999e-60"
     Typing          7468..8195
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.239"
                     /coverage="100.0"
                     /evalue="1.12e-141"
ORIGIN
        1 ctggaagaca tggcggtcat ggcctggcag accggggcat atgtattccg gtttgaagat
       61 caggcccagc atctggcgat gatgaagttt atcggcatgt atcacgaacg gctgaacagc
      121 gatgacaaag ccacgtatta cttcaccggc aaaaatccgg cgtgggaacg tgataaaccg
      181 tccggaatga agctgtgtga ttatctttac cgcaaggttg agcagttcgc catcgagaac
      241 ggcgtgcagg tactggacgc agggaagtac accccggagc agatcggcgc gatgattgac
      301 gtggtgagtg agcggcacgg tgagagtgcg gtgaacgaga agctgaaagc ggagctgatg
      361 cgagagtata acagccggta ttttaacgag ccggtgcccg tattgccgga ggataaaccc
      421 gcccgccggt ggtggccgtt cggcaaaaaa taaacgccag caccggccat gccggtgctg
      481 ttattcttcc ccgagcttac cccgcattac cagccagagt ttttccgcat cctcgtgaag
      541 tcgttcgcac atgtcggccg ggtcgtccat gtccagctcg ttcagctttt ccagcaacag
      601 cagcgactga tccctgatga attttgccat gttgagggcg gttttttcct gcttcgtcat
      661 gattttttcc cttccgggca tggtgtgcgc cagtcgggta tactgtctca aaaatagcgg
      721 ggtgagacaa tggcgtgggt gacggtgaga caggcgacgg aactgacggg gaaagcccgg
      781 agttccctgt accgggatat ggcgaaaggc cgcgtatcat accggacgga ggctgatggc
      841 ggtcgggttg tggataccag cgagcttatc cgggtgtacg gtgagttaag acagggtgag
      901 actcacgagc gggacgggtt gagactgcca gatgagactg aaaaaacggc ctctgaagcg
      961 ttgatcgcgg agataaaagc actcagggag gaggtggccg ggttgcggca ggaaatgcag
     1021 gccatgagac tgctggaaca caaaccggag accgtagaga caccggccga aacgccgcgc
     1081 cggtggtggc attggggaaa acgctgacgc gctgcgcttg tccaaccggc gacgggcacg
     1141 caatgaattg cgcccctgtc accggttatt agcgaacggt atggggattc tggcgctggc
     1201 cgaccacggc cagaacccgg actttctgcg cggggtcgtc cacttcatag aggacatgtt
     1261 gtcccatcag gctgatccgg cgaacgtctt cgccataacg tgagtccact tcgtagaggc
     1321 gcgggaagtc cgccagtttg tccgcagcag cgagaacccg gtcggtaacg ttctgcgcat
     1381 cccacagcga tgaattttcc gcgatatagt caaacagtgt cgttacatct tcgatggctc
     1441 cctgcttcca ttccacgtca tacggcatcg cgcagttcct gataacggcc atgcaggcgc
     1501 tggcggagtt gttccgtggt catgcccgga cgcgtgtcag cacgggcggc ggtcactttg
     1561 gcgcgaacct gatcttcata ctctgcctgg gccagttctt cgttagccag gcgctgcaac
     1621 agcgcagctt tgtccgggcc accttccggc agtatgcggg gcaggctttc agccagttcc
     1681 cgaagaaagt tggcgttttc gtagtgtgtc gtgttgctca ttgcggagcc tcccgggtta
     1741 atgcggcaat atgaaaaagt ttagcaggag gcggcctgaa agaatatgct cgtgtcagtc
     1801 aggctgatga agcagaaagt cggtaaaaaa gggcttacgg catccatttt acgtcaaatc
     1861 ccatgccatg cagttacgtg catagccatg cgttcaggta caatttatcc ggcgcgtttc
     1921 cgggggtgtt gcgggtggtt tgttgtctgt tttatcggtt tcccgtcaga aatgccctca
     1981 gaggctctca gggggtgcgg gcaacggatg ttatggtaaa tgacgggatt gtgaggcatc
     2041 cagctgacga tttttgcgtc aacttttcgc tgtatgttta aacatgagtt taacattagc
     2101 gtcatctgat agcgctgttg ctaatttcat ctacccgaca ggtaatttcc ttgtaaatca
     2161 agcgcgaaag atcagcaaaa aagctatgtg tgcgcttatg gaatagggta gaataaggga
     2221 ttctgatgat cgtgaggttg tcagtgaaaa acgaggcagc tatcagatct tcggcctgag
     2281 aaactaagga ttctgatagc tgccggtgac tcagttcgcg aaaactgaat cttacctgat
     2341 caacaaacgc ccgattaggg cgtttgtcaa gtggtgctgc attatgtagt aacttggtac
     2401 cgacttgcca ggcatttttc acagtttttt gatgtgcgaa ggctcatccg gacaccgtca
     2461 cctgttattt atcctctgta aacaggtgct ataaatgcaa aaagtaaccg atctgtatcc
     2521 tcctgaaatt gctcgccata agctccagaa tcacttcgcc ggtaataccg ttatgctgga
     2581 gttaatccag aaactaaata aaaccagcct gtgtactttt gcggcgctct gtgacggcaa
     2641 cgtcgtgacc acttccggct ataacattac ggccgatctg tgtgttaacc gtgcgtcagc
     2701 cgtggcccat tcgcttaagc aaaaatatct gccggttacg gccagaacga tttcgactaa
     2761 ggccgatgta ggtggtgcgg taaagcaggc ggctttctgt atcgatgaag acgatcttgc
     2821 aaggctgaaa tcagagccgg aaaagatgat gaaagagtgc gaacgtaacc tgaacagcca
     2881 gaagcgaacc aatgctcaga aggaaatgag tcggttgtac aaggagtttg gtgaagacgg
     2941 catactggcg ctgttacgca atgttgcccg gtcgaacggt acaccgccgc caggcgcaca
     3001 ccctgcaagc tgataccagt aaagcccctc aggggcttta tttttaaggt tttctggatg
     3061 ccgccagctc ttcatctgtc agctgatctc ggtgcagata gttccctttt accggcggtg
     3121 gtgtcaccag ttttgctttg ccttctttcg gatcgtatat cgccacaagg ccggtaatgc
     3181 ttccttcctc agtcagtgcc catgcagcaa gctgataaac gatcaccgga taatcagact
     3241 ggtcggtaaa atgacgaaaa taccagtcct gtgcggggat gagttgctta aagttcatgt
     3301 attagctcct tcaccattcc gcttttgtgt ctgcaatgac atagggtttc cacgtcggtg
     3361 atttacacgg gctttccggg taatgtggcg gaaatgtatt gtctctggag tgggcattta
     3421 ccgtcacttc acgattacat ttcgtgcatt tgtagatccc cgagtgtgga acttcatctc
     3481 cggctgaaaa tcgcttaccg aagaagtcat cgctggcgtt tgttgccagt tcattcagcg
     3541 tttttttctg tactaaggcc attctgcttt ccttcccatc gatatttttc gggcattctt
     3601 acccatttta taaatgtggt ctttgtggcg ttttttcaag cggctgagtt ttggttaagt
     3661 caggattatg agtatgatgg cgtgatttta accatttaaa gtacaagaaa ttagtatgga
     3721 gaagcagtac accaatgaac tgaccgcgga gattctggcc ggaatggatc aatcaccttt
     3781 tactcctgaa caactggctg cgatgagcga tgaagcacgc gcgttgattg aggagcagga
     3841 ggcattctgc catgcgcatc cggtgacgac catttaccga ctggcggtgg cgggctgtct
     3901 gacccggcgc ggtggtaccg gcgatgagtt caatccgaac cctgaggaag gccataaaat
     3961 ccggctggag aacgggttgt gggtcagcgt gctgactgaa ggttgtaccg tgacgtatcc
     4021 tgatggcacg caagcccgga ttctgtagga tcttaaaaag atccacttga gatcgtttac
     4081 cactgcgcgt aatctcttgc tctgtaaacg aaaaaacccg ccgtaaggcg ggttagttcg
     4141 ggttcgtcga gcggcaaact cgccgaaacc gggcggtaac tgatcttcgc aggaacagtc
     4201 accaaaacag tacagtcagt ttagctgctt caaactggct tcttcaagcg aaaaagttac
     4261 tttttcactc ttacgttcct gcgaactcca gttaccagtg gctgctgcca gtggcgtttt
     4321 accgtgcttt tccgggttgg actcaagtga acagttaccg gataaggcgc agcagtcggg
     4381 ctgaacgggg agttcttgct tacagcccag cttggagcga acgacctaca ccgagccgag
     4441 ataccagtgt gtgagctatg agaaagcgcc acacttcccg caagggagaa aggcggaaca
     4501 ggtatccggt aaacggcagg gtcggaacag gagagcgcaa gagggagcga cccaccggaa
     4561 actgtgggga tctttaagtc ctgtcgggtt tcgcccgtac tgccagattc atggttgagc
     4621 ctcacggctc ccacagatgc acctaaaaag cgtctgttta tgtgaactct ggcaggaggg
     4681 cggagcctat ggaaaaacgc caccggcgca gcttcgtatt attttctctc tatgcctgtt
     4741 tgccctgctc gtgagtccgc agcgcccgcc gcagtttcac ggccggagcg tagcgaccga
     4801 gtgagcgagg aggcgcaata tctcccgttt ttgcctacgg ccaccggacg cgctgatgtg
     4861 acattttgct cctgattaaa gcctttctgt tccctgtcga taaatcatca ggataggtga
     4921 agtatgtcgg ttttctgata cacattttac gttaacctat ctctgtttct ttttctcctt
     4981 gcggagaaat gcaagtcatc cccgctaggg cgggactgac ttcaggggta aatcccctgc
     5041 ttacgctggc tgaacgccac cgacgcagcg tctttacccc tctttctgtc ttagcccggc
     5101 tggacgccgg tcacgccaga aattcacccg gcccccgtca ggctcacgcc tgaccaccca
     5161 caaaaatcca cggtgctgga cgcacctgac gaagcagcaa agctgctcct gattttatgg
     5221 gtggtcagcc gctcgctgtc gcttactctg acgggggccg gaacaccgga aaagacagca
     5281 ctggggtgcc gccttttcct ctcgcacgct gtgggcgtgc tcgttgttcc tccggggctg
     5341 cgccttttcc gcatggggcg gaaagaggct ggcctccgtg gcgagcgctg aacgctttca
     5401 ccacggacgt gaacgccgcc gccatgcctt ggggcatgtc gtcagcttgc gcagatgggc
     5461 tgcgcatcac gccggtaaat ccgtcgtctg taggagagag gttgcatgtt gaatacggat
     5521 tcgcttgcct ggcagtggca tatcccggag ctggcccggc agatttaccc gtgggtcagc
     5581 tggctgtacc gggagtatta cggtctggcc ggggcttttc tggttatcgg cctgtttttc
     5641 ggtctggctg aactctgggc cggacggatg cgttttgtgg gcctgagcca taaggagcgg
     5701 cgggagtatg ccgatcgcta taagcgcgat aatgacaggg ctgcgccatt ttcggtgctg
     5761 gcctcgctgg attctcccgg gcggatggtc agggctcgtc gacatgtctg gaactacgcg
     5821 tactggctga ccggcgctgc cggtctggtt ctgctggggg ggctggttgt cgccgtgttt
     5881 atcgagagct ggggagaaac ggtgttgcgt ccgggctact acgcgaatgc cgattattac
     5941 ctgctgcaac ccgctaatct gttcgggata gcctggtgtg ccggtggtct ggtcgtcggg
     6001 atacttctgg cgcggctact ggcattcctg ctgcttgacg gtcattttgc ggccgctgac
     6061 gaggcggtca atgcaaaact gaatcaggag agccggcgca gcagccagcg caccggcgaa
     6121 atgactgacg tgcgccacct gcatttcggg gagccggtgc cggtcaacgc gctggccgat
     6181 ttttccacgg agcaggcccg gcagcagcag gtggtgttcc tgggtaagga cgaggacggc
     6241 cagccggtgc gggttccgcg cgaggcgtgg cgcaagacca atattcagat cctcggccta
     6301 cccgggagcg gcaagagcgt gatggccacc aacgcgctga tccgctgcgt gcgcgatttt
     6361 ggcgatgcgg tggtgtactt cgacccgaaa ggcgacgcat gggcgccaca cgtctttcgg
     6421 gcgcactgcc cggacttcac cctgctggac ctgcggccgg ggaaaccggc gcagctgaac
     6481 ctgttccgcg accttgacca gtacgcactg aaaaatctgc tggttgccgg gtttaacctg
     6541 gcggaaaccg gcgatgtggc cgaccactac cgcatcagcg agcagaaagc ggcgaagctt
     6601 atcgcggagc agttcccgca cggcgcgaac attcagcagg tgctggcggc ggcgtatgcg
     6661 ctgccggagg cgctgaaaaa ggacgtgaag gggctgatca ccaagctgga gaacgtcgcg
     6721 gacctgagcg tcctgcagac ggattccggt attgatgtgg cggggattat caacggcggc
     6781 ggctgtctgt acgtcatcgg atcgatggac gacgaggcgg tgatccgggt gcagaaaatg
     6841 ctgtttgccc gctgcgcgca aatcatcatt gcgcgggacg agttccgcgc gtggccgcac
     6901 gtcagcgtga tgctcgatga aatcaaatat ctgctctcga agtacgtact caacgcgctg
     6961 gggacgctgc gcagccggga ctgtaacctg ctgctggctc accagtcgct gggcgacttc
     7021 ggccagtgcg ggcaggatct gcccgctgat tttgtgaaaa ccacggtact ggataacacg
     7081 ccaatccgct ggttctatcg cgcggccagc caggagtccg cgcagtgggc ggccggacag
     7141 accggggaaa tccgggtgga cgtggagcgc cgccgggcca gccgggaggc ggggaacgtg
     7201 gagcacatca gcggggacag cttcatccag aaggaagcgc ggccgctgtt cgacgtgaac
     7261 accctgcaac acctgccgga cgggttcgcg gtcatgaccg ggctgggcgt ggcgcggctg
     7321 gcgttcagca gcccgctgcg ggttgatcgc cgggaaattc cgctgaaaac cttcccggtg
     7381 ctggcgaaaa cggacccgct ggcggagtac caggctgagg cagtgcggca gcgcccgggt
     7441 gacgatgatt ttgcggggct gtactgatgg ctctggagca atacaacgtc agccacgcga
     7501 agcgacaggc gcggaacgcg gagaaaaccc ggctagtcct gcggtggctg cgggacgaac
     7561 tctgctcgac ggctgaaacc gtagcccgtc tgctggggat cggggcggtg cagccggttt
     7621 accgctttct ggacagcctg gtggcgaaag gactgctggc ccgggcgaaa tacccggttg
     7681 acggtcgtca ggtcagcgtg tgggggctga ccccgcacgg cgtggcgttc tcgttcgacg
     7741 aggacgagcc gctgacggac gttattccgt ttcagccctc gcgggtgtcg gcggcgcagc
     7801 tgccgcaccg gctggccgtg cagtccctgc gtcttgatat ggaagccaga ggggccaccg
     7861 gctggcgcac cctgcaccgc ctgagtctta aaggtatgaa ggtgccggat gcgctggcag
     7921 agctggacgg taaaacggtg gcgtttgagg tggagcgcac cgtcaagagc cgtcgccggt
     7981 atcaggaagt ggtgtccggg tatctgttca accgtaaggc gaacggcatc gatgaaatct
     8041 ggtacatctg ccccgaccgg ccgacgcttg tccgggtaca gcgggcgatt ctggcggtgg
     8101 atgaaatcgt gaacccgcag accggcgaag cccgcaaaac ggcggaactg gaccgggaga
     8161 ggctgttcgc gtgctttaaa tttatgacaa cagagtgagg gattatggcg ctgtacatgg
     8221 tttttattga ggatgtggaa gacaaagacg gcgagcgctg cattatgcca accagcgagc
     8281 at
//
