LOCUS       NZ_CP073311             1334 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterobacter cloacae strain CZ862 plasmid pCZ862_1, complete
            sequence.
ACCESSION   NZ_CP073311
VERSION     NZ_CP073311.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18753389
            Assembly: GCF_018140965.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Finianos,M., Kraftova,L., Papagiannitsis,C.C., Adamkova,V.,
            Hrabak,J. and Bitar,I.
  TITLE     Genomic characterisation of three GES-producing Enterobacterales
            isolated in the Czech Republic
  JOURNAL   J Glob Antimicrob Resist 29, 116-119 (2022)
   PUBMED   35231657
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial assembly v. 1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 670X
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018140965.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 04:38:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,479
            CDSs (total)                      :: 5,360
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 53 of 288
            Pseudo Genes (incomplete)         :: 204 of 288
            Pseudo Genes (internal stop)      :: 51 of 288
            Pseudo Genes (multiple problems)  :: 20 of 288
            Pseudo Genes (short protein)      :: 1 of 288
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073311.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="CZ862"
                     /isolation_source="Hospital environment"
                     /db_xref="taxon:550"
                     /plasmid="pCZ862_1"
                     /geo_loc_name="Czech Republic: Prague"
                     /lat_lon="50.07 N 14.43 E"
                     /collection_date="2019-12-05"
                     /collected_by="VFU"
     gene            748..1242
                     /locus_tag="KDU74_RS26785"
                     /old_locus_tag="KDU74_26785"
     CDS             748..1242
                     /locus_tag="KDU74_RS26785"
                     /old_locus_tag="KDU74_26785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="WP_045289185.1"
                     /translation="MLVSTETQKKQRLLDLQEKDRKNASESLQEQKNANFTQVYPLGWK
                     RLRELFRLNSGAAELYSMLAENIDGACGAVVADQAYLASLMGVSRQTISKYIKFLESQL
                     VLVKIPVAGKVCAYALNPHEVWKGYDNAKPHAAFLTKTLVNKDGDIQRRIMAMFAGKDD
                     E"
                     /name="HTH domain-containing protein"
     Typing          268..348
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.976"
                     /coverage="93.0"
                     /evalue="3.98e-26"
ORIGIN
        1 ttttgaggct taccacgaag cgaatggtca attgaattat gcggtatatc aggctctaca
       61 cccgcttttg agagcgcaaa ggggtcaagg ttcgccatcg gcgaccgcga agcggcctga
      121 ccttgacggc ttggagtgaa ccccaccatt ggcgaaggtg gttggtttca cgaagtgagg
      181 ccaaccacct gttaagccgc ccttgacgtt gaccttcccg aacatcagaa atccctgcga
      241 aaacccctca gaatcggtgc gttctgccgt gccaaaagct tagtaagcca gtatacactc
      301 cgctagcgct acgtgactgg ttcagggctg cgccccgaaa cccgcaaagg tcaaaaccaa
      361 aagcgtctgg caggtggttg cgctcactgc gtgatcacaa ccaccgccgg gtagtgtggt
      421 tggtcttttt tggcgtcaag gtcaggccgc ttcgcggtcg ccgatggcga accttgaccc
      481 caaaaatgcc cgaccaaagg gagggagcta ctccgaagat cccggtgcga gcatcgggat
      541 ctttagataa gaagggaatt gtttgtatgt tattgttttt tattatattt catatttacg
      601 ttgtaaactt atagggttta caacgttgta aagctctatg gggtttacag attttaacag
      661 catgtaacca aaacgcgaca tcttgtaaac caaaaacgag ggtgttatgt tcggtctagg
      721 ttacatcatg taaacagggg tggttgtatg ttagtcagta cagagacgca aaaaaaacaa
      781 cggctgttag atctccagga gaaagaccgt aaaaacgcct cagagagcct acaagagcaa
      841 aagaacgcaa acttcaccca ggtataccct ttgggctgga aacgtcttag agagcttttc
      901 aggctcaatt caggggcagc agagctgtac tctatgcttg cggaaaatat cgatggtgcc
      961 tgcggtgcag ttgtagcaga tcaggcatat ctagcaagtt tgatgggtgt ttcaaggcag
     1021 acaatatcta aatatatcaa attcttagag tcgcaactgg tgctagttaa aataccagtt
     1081 gcaggcaaag tttgcgccta tgcactaaat ccccatgaag tctggaaagg atatgacaac
     1141 gccaagcctc atgcggcttt cctaacaaaa acactggtca acaaagacgg tgacatccag
     1201 cgaagaatca tggcgatgtt tgcaggtaaa gacgatgagt gaagccgcag acacactaaa
     1261 atcggctctc cgactgatta cagagatgat cgacatactt gaaaatagcg atgatgcaga
     1321 agctccccaa gcat
//
