LOCUS       NZ_CP073304            10060 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SW1780 plasmid pB, complete sequence.
ACCESSION   NZ_CP073304
VERSION     NZ_CP073304.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18793569
            Assembly: GCF_018140905.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Ding,M. and Zhou,Y.
  TITLE     Study on the mechanism of subinhibitory concentration antibiotic
            stress mediated conjugation frequency of Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Ding,M. and Zhou,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2021) Basic Medical College, Southwest Medical
            University, 1 Xianglin Road, Longmatan District, Luzhou, Sichuan
            646000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. November-2020
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 10:28:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,633
            CDSs (total)                      :: 5,511
            Genes (coding)                    :: 5,386
            CDSs (with protein)               :: 5,386
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 46 of 125
            Pseudo Genes (incomplete)         :: 82 of 125
            Pseudo Genes (internal stop)      :: 12 of 125
            Pseudo Genes (multiple problems)  :: 15 of 125
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073304.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SW1780"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pB"
                     /geo_loc_name="China"
                     /collection_date="2019"
     gene            complement(join(8506..10060,1..410))
                     /locus_tag="KCX81_RS27910"
                     /old_locus_tag="KCX81_27910"
     CDS             complement(join(8506..10060,1..410))
                     /locus_tag="KCX81_RS27910"
                     /old_locus_tag="KCX81_27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(1894..2043)
                     /locus_tag="KCX81_RS27915"
                     /old_locus_tag="KCX81_27915"
     CDS             complement(1894..2043)
                     /locus_tag="KCX81_RS27915"
                     /old_locus_tag="KCX81_27915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(2128..2385)
                     /locus_tag="KCX81_RS27920"
                     /old_locus_tag="KCX81_27920"
     CDS             complement(2128..2385)
                     /locus_tag="KCX81_RS27920"
                     /old_locus_tag="KCX81_27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(2395..4080)
                     /locus_tag="KCX81_RS27925"
                     /old_locus_tag="KCX81_27925"
     CDS             complement(2395..4080)
                     /locus_tag="KCX81_RS27925"
                     /old_locus_tag="KCX81_27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            4407..4652
                     /locus_tag="KCX81_RS27930"
                     /old_locus_tag="KCX81_27930"
     CDS             4407..4652
                     /locus_tag="KCX81_RS27930"
                     /old_locus_tag="KCX81_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            4926..5297
                     /gene="tolA"
                     /locus_tag="KCX81_RS27935"
                     /old_locus_tag="KCX81_27935"
     CDS             4926..5297
                     /gene="tolA"
                     /locus_tag="KCX81_RS27935"
                     /old_locus_tag="KCX81_27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            5294..5659
                     /locus_tag="KCX81_RS28225"
     CDS             5294..5659
                     /locus_tag="KCX81_RS28225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            5834..6112
                     /locus_tag="KCX81_RS27945"
                     /old_locus_tag="KCX81_27945"
     CDS             5834..6112
                     /locus_tag="KCX81_RS27945"
                     /old_locus_tag="KCX81_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            6100..6414
                     /locus_tag="KCX81_RS27950"
                     /old_locus_tag="KCX81_27950"
     CDS             6100..6414
                     /locus_tag="KCX81_RS27950"
                     /old_locus_tag="KCX81_27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6578..7006
                     /locus_tag="KCX81_RS27955"
                     /old_locus_tag="KCX81_27955"
     CDS             6578..7006
                     /locus_tag="KCX81_RS27955"
                     /old_locus_tag="KCX81_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            7032..7211
                     /locus_tag="KCX81_RS27960"
                     /old_locus_tag="KCX81_27960"
     CDS             7032..7211
                     /locus_tag="KCX81_RS27960"
                     /old_locus_tag="KCX81_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7238..7768)
                     /locus_tag="KCX81_RS27965"
                     /old_locus_tag="KCX81_27965"
     CDS             complement(7238..7768)
                     /locus_tag="KCX81_RS27965"
                     /old_locus_tag="KCX81_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7775..8506)
                     /gene="mobC"
                     /locus_tag="KCX81_RS27970"
                     /old_locus_tag="KCX81_27970"
     CDS             complement(7775..8506)
                     /gene="mobC"
                     /locus_tag="KCX81_RS27970"
                     /old_locus_tag="KCX81_27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     BGC             1..9080
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1213..1791
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7779..8506)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 tccgggcgca ggacggtttc tccccagctc tcgataaaca cggcgacaac cagccccccc
       61 agaagggcca gaccggcagc gccggtcagc cagtacgcgt agttccagac atgtcgccgt
      121 gccctgacca tccgcccggg tgaatccagc gaggccagca ccgaaaatgg cacagtccgg
      181 ttattatcgc gcttatagcg atcggcatac tcccgccgcg ccttatggct cagccccaca
      241 aaacgcattc ggccggccca gagttcagcc agaccgaaaa acaggccgat aaccagaaaa
      301 aaaccggcca gactgtaata ctcccggtac agccagctga cccacgggta aatctgccgg
      361 gccagctccg ggatatgcca ctgccaggca agcgaatccg tattaaacat gcaatctctc
      421 tccttcagac gacgggttta ccggcgtgat gcgcagcccc cgctgcacaa gccgcggaca
      481 tgccccaagg catggcggcg gcggtcacgt ccgtggtgat tgcgttcagc tttcaccgcg
      541 gaggccagcc tctttccgcc ccatgcggaa aaggcgcagc cccggaggaa caacgagcac
      601 gccccagcgt gtgagaggaa aaggcggcac cccagtgcgg tcttttccgg tgttccggcc
      661 cccgtcagag ttcgccatag cgagcggctg accacccgca aaatcaggag cagctttgct
      721 gcttcgtcag gtgcgtccag caccgtggat ttttgtgggt ggtcaggcgt gagcctgacg
      781 ggggccgggt gaatttctgg cgtggccggc gtccagccgg acagagccag aaagagggga
      841 aaagaccgtg cgtcggtggc gtccagccag cgtaagcaag gtcttttccc ctgaagtcag
      901 ccacgcccca gcggggatga cttgcatttc tccgcaagga gaaaaaagca acagagatag
      961 gttaacgtaa aatgttcagc gaaaaaccga catggttcac ctatcctgat aattgatcgt
     1021 cagggaatag aaagacttaa tcaggggaca atatcccaca tcagcgcgtc cggtgaccgt
     1081 cttgcaggtg ccggggaaat tgcgcctcct cgctcactcg gtcgctacgc tccgggcgtg
     1141 agactgcggc gggcgctgcg gacacataca aagttaccca cagattccgt ggataagcag
     1201 gggactaaca tgtgaggcaa aacggcaggg ccgcgccggt ggcgtttttc cataggctcc
     1261 gccctcctgc cagagttcac ataaacagac gctttttcgg tgcatctgtg ggagccgtga
     1321 ggctcaacca tgaatctgac agtacgggcg aaacccgaca ggacttaaag atccccaccg
     1381 tttccggtag gtcgctccct cttgcgctct cctgttccga ccctgccgtt taccggatac
     1441 ctgtccgccg ttctcccttg cgggaagtgt ggcgctttct catagctcac acactggtat
     1501 ctcggctcgg tgtaggtcgt tcgctccaag ctgggctgta agcaagaact ccccgttcag
     1561 cccgactgct gcgccttatc cggtaactgt tcacttgagt ccaacccggg aaagcacgca
     1621 aaacgccact ggcagcagcc attggtaact gggagttcgc agaggatttg tttagctaaa
     1681 cacgcggttg ctcttgaagt gtgcgccaaa gtccggctac actggaagga cagatttggt
     1741 tgctgtgctc tgcgaaagcc agttaccacg gttaagcagt tccccaactg acttaacctt
     1801 cgatcaaacc acctccccag gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa
     1861 aaaaaggatc tcaagaagat cctttgatct tttctactga accgcgatcc ccgtcagttc
     1921 agaggaggac gatggtgcca ccgttccgcc ctgaacatcc cggatataat ttgcctgaca
     1981 tgcggccaga agaaagccag caacaatcaa tataaaaaga aaaatagtct ttaccttttt
     2041 catatacttt ccctgtattt aaagactccg gcattcatcc ctgaacaccg gaagtcagta
     2101 tttcaaatca tccttgataa ttttttgtta ccagttaccg tcccgataat caaaagctat
     2161 ataacaatta tccacatcag gttttatttg atttttaaaa aatggctgta gtaaaggaac
     2221 ccattctttc ttcaaatcaa actcaccatt attaatatta tcctttaaag gcattcccaa
     2281 gctttcaatg acagaaccat cgtcaccaaa gtctttagaa tactcttccc ctttaaactc
     2341 ttcagttttc ttatcaaacc atgtcaaacg taatttaagc cccatattta cctctcaaag
     2401 atatttttta atgttccgtt ttggatctgg ccctttaacc tgcttacccg ttttgggatc
     2461 aaatgcgcct aggtgttcgc catcactggc acgataccct tcaagctcac cgtgctggga
     2521 atcccattca taaattttac gttttttatc gccataccat cgggcccgct taccaccacc
     2581 accttgttta ggtgttttag gatcacctcg ctttaactct ccaagtccct ttatgtcttc
     2641 tgttttaggt actggatgat aatcatggcc gtagtcttta gtccccttgc gaggcttgtt
     2701 cttttcctca tttaacttat tttctgcatc cttcgctttt ttctcctttt gtttccggct
     2761 ctccatcgcc ccggtaagtg cagccttatc tctggataag gattcattca ttgcgtttaa
     2821 agatgcctga gccgctttta atttctcatt ctgattctga atatccttat caagatagcg
     2881 aacctgatcc tgccacccgg agccatattc atgaggcggg ttataagtca caaaatcatc
     2941 tttatttgcc tctgcctctt ttaccttttt atcggcttcc tgaacagcag cccttgcacc
     3001 ggggagcgcc ttctctgtcg atgcaatgcg gctgtttaag gtcgcaatat ttttatcttc
     3061 ggcatccagc tcagctttcg ctttatcata gtctcttttc agcccctctt ccggatgagc
     3121 ggcgtcccat gcctgctggc gacgcttttc ttcctcctga cgctgtttta cctgtgccgg
     3181 ggtaagaaca tctgtcaccg acacataaac cggcttctgt ccggtctctt tcgggaaacg
     3241 aataacggcc tcacgggagt taccaccggc tgtaaaacca gccggacgtg aatcaccttt
     3301 ttcagcaaca atgccttttg gcggggcttt cgctgtcgga acacctttcg gtacgctcac
     3361 ctgcagagac gggagacccg gaatcgacac actgaatacc cccggacgtt ttgtcggttt
     3421 cgcatccacc acagggacac tcattggccg acctgcgaca accgcaatat gctgccgctc
     3481 atccttcacc acatccacaa cccgttgtct gacacgaacc gtcgcctggt ccagtggtaa
     3541 agtacttgcc ggagtctccg tcaccgcatc ggccggtaat gacgtcataa tttttgacat
     3601 cattttcgga tcatcttttg caatctcaga aggcagcaca ccgtagatcg caatccccca
     3661 cagaccaaac ttaaacggcc ccttcagggc agccagcaca tcagcaacgg cggccgacaa
     3721 cgcatcgccg gaaactgata aagccagtcc ttcagccccc ggagtagcca gggcaggcag
     3781 accgaaggcc atcgcagtgg ccgaagactg tccgccacca gagctaccgc tatgattgcc
     3841 gctgttacca ccattaccac cgggtttact cccgccgtta ccaaactccc ccggattata
     3901 gttatgaata tctccattcg gcgtatgcgt cgtaccccag cccggcccgc tgtttggatc
     3961 cgtaccacca cctgatgaag ggccaccttt accagacgta ccgttcacat tcccactggc
     4021 ctgaccacca ttatgtccca gacctgaatt acccggacct cgaccgtctc caccactcat
     4081 accgttttcc tctttgacat ttaaaacaat aaattaaagc caacatactg tacatataca
     4141 cagtataatt attaccttac tcctcccgtt ttcaggtgtc aatatatgag attagttctc
     4201 atcttcaggc acaaaaaagc gcccggagcc acctgtaagc agatcgcgtc ttttcccgaa
     4261 aatttctcat ggcaacaaat tttattattt aagacattca tcacacttac atttcagatg
     4321 gtatagacac atctgaaagc taactggcat gaggagagga cctgcggtta aactgttcgg
     4381 gaaacctgag acgcccggag gccactatga cgcacctgaa actgactccc gctgaagaaa
     4441 agcgcctggc ggacgccttt gtcgctcagg cactggccat gggcctgaaa gggaacaaaa
     4501 cgctgccaaa ggcggtaaaa caacaggtgc gggaaagggt gcttcgtgcc attcaggacg
     4561 aacgtgaagc caggcgggcg caggaaatgc gtcgggagaa atacgaggct acggaaacca
     4621 ccttcacctg gcagccacag cgccgccggt aaggggtatt taccataacg tcgcattgcc
     4681 cgcaccgctc agacggtctg agggcgtttc cggcggctac gggataaaac aggcaacaaa
     4741 ccacccggaa aaccgccgga aacgcgcccg gtaaattgta acccgctgca tgcctatgca
     4801 cgcaactgca tggcgggccg tttggtgtaa aaacgatacg ataagccgtg tttatgtaaa
     4861 tgaacgtggc cgtaaagagt gacaaatata catgtaacat tttttactga caggtcatca
     4921 acccgatgcg tttaataccc tttatcctgc tggcattgtg cagcccctta ctcctgacgg
     4981 cctgtaagca ttccaccacc cagccgccga aaatttatac tctggataag tcatctaaaa
     5041 tcagtagcta cgctgcggcc atcaagaccg ctgttgaaat gcagatgttt gaaccagagg
     5101 cctatgcggg aaaaatgtgc accgtgcgga tatccataca acgtgatggc actctgaaca
     5161 gcgccattgc tgaaggagga gatccgaaac tctgtaaagc agcaatatca gccgttaccc
     5221 acgccaaaat tccgccggca ccggatgaaa atacctggca aatattcaaa aacgcccctc
     5281 tggatttcag accatgaaag cttttactct gacagtgctg gcggccatgt tcctcactcc
     5341 ggtacatgcg ataccactaa aacccgtaac cacactgcag ccaacctacc cggtacaggc
     5401 cgcagcaaaa cgcatcgaag gtcaggtgac cgccacgttt gatattctgg acgatggacg
     5461 agtggacaac gtgtcgctta ccggctgtca cctttttttt cgggaaacgc tgacggcaat
     5521 gaggcactgg ctatatcccg cagggcaccc ggcaatgcga caggttataa attttgaatt
     5581 cagtctgaaa aatgacggct tacaggatcc gcgaccagtc accacccaaa agctcaatga
     5641 tgatggttgc cgccagtaac aggtccttta acccgcaggc tgcaatagtc ctggatgatg
     5701 agtgagcctg tagcagccct gattagtgga agcgaagctg aaaaatgggc agcaaaagcc
     5761 gtatttctgg caaatagcgc cgccacggat taaagtagtc atgtgtaata catatgacta
     5821 caaaggggct tttatgtcat cagcaacatc catcaggctc gatgaagaac ttaaggaccg
     5881 gctgaaaacg ctggccgatg accggcaccg ctcagcgcac gcgctaatgc tggaggcaat
     5941 caccgaatac atcgaccggg aagagaagcg cagccagtac ctgcgggacg gacaggccgc
     6001 atggcagcac tatcaggaaa cgggtctgca ccttactgcg gaagaagcgg aagcctggat
     6061 aagtacctgg ggaactgaga acgagcagga tgctccgcca tgccacaggt aacgatttcc
     6121 gcgctggcac agcgcgattt acaacgcctt caggactttc tcaaaaccaa aaatcggctg
     6181 gcagccagaa aaggaggtga ggtgatcgtc cgggctatcc agcaactgaa aacattgcca
     6241 gacattggcc gcccggtgcc gtttctgccg ctggaatata aggaactggt gatcgggttt
     6301 ggcgacagtg gctatgtgat gctctaccgc cacgaccggg aaatggacca gattgtgatc
     6361 gtcaccgtca ggcatcagaa agaatccggg tatccggggg cagacagcct ctaataaccg
     6421 atgacggggg cgcaattcat tgcgtggccg tcgccggttt gacaagcgca gcgcgtcaga
     6481 aaaccacatc ctgaacggaa aaacgggtaa aattcagcga tacatttacc gtgagaaatt
     6541 acacaccttt cacacccgat aaaatcaggg gtatacagtg gcgaaaacat tgctcaacct
     6601 ttcacaggca gcacaagccg cgggcattac cagaagaacg ctatacaatc atgtaaaaca
     6661 gggaaaggtt accgtgtcac gtgacgggaa aaacaaccct gtcgtggatg tgtcagaact
     6721 gatccgtgta tacggaaatg tgaatatccc tgagaaacaa attcccggca tttcacaccg
     6781 tgaaaataca caaaaaaatt tcccacagga acagcttctg gcaatgcaaa aggaactggc
     6841 cgatctccgg caggctgtca ccctgatgct ggaagataaa acatcccggg aagaagaacg
     6901 ccgccagcac gatgatgagc gccggaaact ccaggccgaa gtggacagat tgacaacaga
     6961 actgacacag aagaaaaaac gcttctggtc cggctggttt agctgagaac ttaccgaagg
     7021 gaatggagaa catgacgaag caggaaaaaa ctgccctcaa tatggctaaa ttcattaagg
     7081 atcagtcgct gctgttgctg gaaaagctga acgagctgga catggatacc ccggccgaca
     7141 tgtgcgaacg gcttcacgag gatgcggaaa cactctggca ggtgatgcgg gataagctcg
     7201 gggaagaata acagcaccgg ccatgccggt gctggcgtta ttttttgccg aacggccacc
     7261 accggcgggc gggtttgtcc tccggcagtt caggcaccgg cacgttaaaa tagcggctgt
     7321 tatattcccg catcagctcc gctttcagct tctcgttcac cgcgctttcc ccgtgccgct
     7381 cgctgaccac ctcaatcatc gcgttaatct gctccggtga gcacttcccg gcatcaacca
     7441 cctgcacacc gttctcgatg gcgaaccgct ccacctttcg atagaggtaa tcacacagct
     7501 tcattcctgt gggtttgtcc cgttcccatt gcggattttc accggtgaaa aagtacgtgg
     7561 ctttttcgtc gctgttgagc cgctcgtcat acatcccgat ggatttcatc atcgccagat
     7621 gctgggcccg gtcgacaaaa cagaaaacat acgccccggt ctgccaggcg agcaccacca
     7681 tgtcttccag atgctcgctg gttggcataa tgcagcgctc gccgtctttg tcttccacgt
     7741 cctcaataaa taccatgtac agcgccataa tccctcactc cgttgtcata aatttaaagc
     7801 acgcgaacag cctctcccgg tccagctcca ccgttttgcg ggcctcgccg gtctgtggat
     7861 tcacgatttc atccaccgcc agaatcgccc gctgtacccg gacaagcgtc ggccggtcag
     7921 ggcagatgta ccagatttca tcgatgccgt tcgcccggcg gttgaacaga tacccggaca
     7981 ccacttcctg ataccgcctg cggctcttga cggtgcgctc tacctcaaat gccaccgttc
     8041 tgccgtccag ctccgccagc gcgtccggca ctttcatccc tttcagggcc atacggtgca
     8101 ggtaccgcca gccggtggcc ccccggccct ccatggagag gcgcagggac tgcacggcca
     8161 gccggtgcgg cagctgcgcc gccgacaccc ttgacggctg aaacggaata acgtccgtca
     8221 gcggctcgtc ctcgtcgaac gagaacgcca ccccgtgcgg cgtcagcccc cacacgctga
     8281 cctgacggcc gtcaaccggg tatttcgccc ggaccagcag ccctttcgcc accagactgt
     8341 ccaggaagcg gtaaaccggc tgtaccgccg caatccccag ccgtcgcgca accagctccg
     8401 ccgtggaaca cagctcttcc cgcagccagc gcagggtcag ccgtgttttc tccgcgttcc
     8461 gcgcctgccg cttcgcgtgg ctgacgttgt actgctccag tgtcatcagt aaagccccgc
     8521 aaaatcatca tccgcttttg gtccgccgcc gtcggccgga ggcaggtact ccgccagcgg
     8581 gtccgttttc gccagcaccg ggaaggtttt cagcgaaatt tcccggcgct caacccgcag
     8641 cgggctgctg aacgccagcc gcgccacgcc cagcccggtc atcaccgcga acccgtcagg
     8701 caggtgttgc agggtgttca cgtcaaacag cggccgcgcc tccttctgga tgaagctgtc
     8761 gccgctgatg tgctccacgt tccccgcctc ccggctggcc cggcggcgct ccacgtccac
     8821 ccggatctcc ccggtctggc ccgccgccca ctgcgcggac tcctggctgg ccgcgcgata
     8881 gaaccagcgg atcggcgtgt tatccagtac cgtggttttc acaaaatcag cgggcagatc
     8941 ctgcccgcac tgaccgaagt cgcccagcga ctggtgcgcg agcagcaggt tgcagtcccg
     9001 gctgcgcagc gtccccagcg cgttcagcac gtacttcgac agcagatatt taatctcatc
     9061 gagcatgatg ctcgcgtgcg gccacctccg gaactcatcc cgcgcaatga tgatttgcgc
     9121 gcagcgggca aacagcatct tctgcacccg gatcaccgcc tcatcgtcca tcgagccgat
     9181 gacgtacagg cagccgccgc cgttgattat ccccgccaca tcgataccgc tgtccgtctg
     9241 caggacgctc aggtcggcca cgttctccag cttggtgatc agccccttca cgtccttttt
     9301 cagggcctcc ggcaacgcgt acgccgccgc cagcacctgc tgaatgtttg ccccctgcgg
     9361 gaactgctcc gcaatcagct tcgccgcttt ctgctcgctg atgcggtagt ggtccgccac
     9421 gtcgccggtc tcactcaggt taaacccggc aaccagcaga ttcttcaggg cgtactggtc
     9481 gagatcgcgg aacagattca gctgcgccgg tttccccggc cgcagatcca gcagggtgaa
     9541 gtccgggcag tgcgcccgga agacgtgcgg cgcccacgcg tcccctttgg ggtcgaagta
     9601 caccacagcg tccccgaaat cgcgtacgca gcgaatgagc gcgttggtgg ccatcgcgct
     9661 cttgccgctc ccgggcaggc cgaggatctg aatattggtc ttgcgccagg tatcacgagg
     9721 gaccagcacc ggctggccct gctcatcctt acccagaaac accgcctgct gtttccgggc
     9781 ctgctccgtg gaaaaatcgg ccagcgcgtt aaccggtacc ggctccccga aatgcaggtg
     9841 gcggacgtct gtcatttctc cggtccgctg gctgctgcgc cggctctcct gattcagttt
     9901 cgcgttcacc gcctcgtcag cggccgcaaa atgaccatca agcagcagga acgccagcag
     9961 ccgcgccaga aggacgccgg cgacaagccc tccggcacac caggctatcc cgaacaggtt
    10021 agccggctgc agcaggtaat aatctgcgtt cgcatagagg
//
