LOCUS       NZ_CP073053             4036 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Citrobacter sp. MGH105 plasmid unnamed5, complete sequence.
ACCESSION   NZ_CP073053
VERSION     NZ_CP073053.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03280165
            Assembly: GCF_001037485.2
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. MGH105
  ORGANISM  Citrobacter sp. MGH105
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Multi-genus dynamics of carbapenem resistance within hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2021) Infectious Disease and Microbiome Program,
            Broad Institute, 415 Main Street, Cambridge, MA 02142, USA
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 4036)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2020) Infectious Disease and Microbiome Program,
            Broad Institute, 415 Main Street, Cambridge, MA 02142, USA
REFERENCE   4  (bases 1 to 4036)
  AUTHORS   Earl,A.M., Onderdonk,A.B., Kirby,J., Ferraro,M.J., Huang,S.,
            Spencer,M., Fodor,A., Hooper,D., Dekker,J., O'Brien,T., Quan,V.,
            Gombosev,A., Delaney,M., DuBois,A., Ernst,C., Kim,D.S., Rossman,W.,
            Gohs,F., Petruso,H., Nozar,T., Mougeot,F., Manson-McGuire,A.,
            Young,S., Abouelleil,A., Cao,P., Chapman,S.B., Griggs,A., Priest,M.,
            Shea,T., Wortman,I., Wortman,J.R., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genomics Platform, The Broad Institute Genome
            Sequencing Center for Infectious Disease
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2015) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.3
            Assembly Name                     :: MGH105
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 159x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:23:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,378
            CDSs (total)                      :: 5,259
            Genes (coding)                    :: 5,190
            CDSs (with protein)               :: 5,190
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 18 of 69
            Pseudo Genes (incomplete)         :: 45 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 13 of 69
            Pseudo Genes (short protein)      :: 1 of 69
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073053.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Citrobacter sp. MGH105"
                     /mol_type="genomic DNA"
                     /strain="MGH105"
                     /host="Homo sapiens"
                     /db_xref="taxon:1686380"
                     /plasmid="unnamed5"
                     /collection_date="2014"
     gene            complement(220..1632)
                     /locus_tag="SK35_RS26785"
     CDS             complement(220..1632)
                     /locus_tag="SK35_RS26785"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048216463.1"
                     /translation="MIRLKKVFNLTEEEIVLTNEIAEAISKEPKVETHEVNINFIDVLP
                     IIPNPHNLGLSENIDPLSLIYSKFVSETDIPPALPIFSFLSYLSAFSVNNNIMYKHPTS
                     PADYLNTWTLILAPSGAAKTTSAKIIESSIPKDIEEKPMIRPNFEGADGSAAFISELAK
                     AEKKIDNFGKPIQPIFWIEDEYSQFMKKLMPGGSMVETRKTMLKIHDNDKARRVTKNET
                     IETESIVMSGLFLNTIDSFARNFDQESINDGLGRRHNFVYAERGEKVVPTWTVEEIIES
                     LKEGLDNFFSTVKTNVIYTYSPECRKIYDHFYMVYKEKFDHILGEETNGTFFRTYFMLS
                     WKYAAIYHILLKEEGTEIQAKSFDYGIKVSLMFLSSIKRFLDYKVDLKSGAKEVRKEYL
                     KKAAEKELNNLQKYKDFLLDNVEITLRDFNRKFSLKKVESLKVLKTIRENKLIKYHPLF
                     EVLAKEEAKAKK"
                     /name="hypothetical protein"
     gene            complement(2229..3647)
                     /locus_tag="SK35_RS26790"
     CDS             complement(2229..3647)
                     /locus_tag="SK35_RS26790"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_139156983.1"
                     /translation="MYHKIIGGKKSKGQRNIKKSIEYLLREKNPEQQKDIKILSTTTKQ
                     DLINFENYILDKNKSNPYVCGVLSFEEKSISEELKTKLITEFEDILFAGIEQENRPPVM
                     WVEHNDKGRVELNYLTFNSLQDRRSYTVYLDKRDRGLVNNYSEIINYENNLSSPFEEKE
                     NKNTLTNKPKTNIPEDKKQFIENINTEIMALIINEELENRDQTIKHIENMKGVKINRIR
                     KNQISIKSDLFDDDKPIVLKGDIYEEGRDYSDYRKEFNPKPTRDPEFVREKLTELKRTF
                     AERIEKREIRNRSKFKKPSFKNISENRQSIQREDQDIGYKYTNGDFINVSNNDGFSSMY
                     IRTDNMEIVNNESINTEYKTKTATEETAGTTRARDIEKINQQQQFVSSRLEEAGRKQQQ
                     FRKSIDTTEGRFGVCRRSVHSIISTFGRYIRTFSAYTRKNEHRQKFEKRRKIEEEKLKI
                     KNQGSYNRRRYKPY"
                     /name="hypothetical protein"
     gene            complement(3649..>4036)
                     /gene="mobC"
                     /locus_tag="SK35_RS26795"
     CDS             complement(3649..>4036)
                     /gene="mobC"
                     /locus_tag="SK35_RS26795"
                     /inference="COORDINATES: protein motif:HMM:NF039635.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_215268669.1"
                     /translation="PFNPLKTEHRRLTVSYKDRYHRKHSVTLCFNDEEFAQVEELASML
                     KKPRAKAIRELILNKEFKLKKEISKNENGDVYYQLHKIGVNINQIARAINTDIDKFISH
                     EFAEDFINLFEEITNEIEDIKRRI"
                     /name="mobC"
ORIGIN
        1 gaacccctca caagcgacaa tacgaagtat tgtctgagct tgctgttata tagttttcca
       61 cttatatatt aattaataca tataaaaatt aagtcaatag ctgaataaaa aataatcaat
      121 ttaaatttgg gttttatata taaccgtgaa caaataaagc cattttgcaa atggcttttt
      181 tgtgaggggt tgaccaactg aacgaagtga aggcggtagt tattttttcg ctttggcttc
      241 ttcttttgcc aaaacttcaa acaatggatg gtattttatt aatttgtttt ctctgattgt
      301 tttaaggact tttaaactct caaccttttt aagactaaat tttctattaa aatctcgcaa
      361 tgtaatttct acattatcaa gcaaaaaatc tttgtatttt tgcaagttgt ttaattcttt
      421 ttctgctgct ttttttaaat attcctttct aacttctttg gcaccagatt ttaaatctac
      481 tttgtaatct aaaaatcttt ttatactgga taaaaacatt aatgaaactt ttatgccata
      541 gtcaaaagat tttgcctgaa tttcggttcc ttcttctttc agcaaaatat gatatattgc
      601 tgcatatttc caacttaaca taaaatatgt tctgaaaaat gttccgtttg tttcctcccc
      661 taatatgtga tcaaattttt ctttgtaaac catataaaaa tggtcataga tttttctgca
      721 ttctggtgaa tatgtataaa ttacatttgt ttttacagtt gaaaagaaat tatctaatcc
      781 ctctttgagt gattcgatta tttcttccac tgtccaagtt gggacaactt tttcacctct
      841 ttctgcataa acaaaattgt gtcttcttcc taatccatca ttaatacttt cctgatcaaa
      901 gtttcttgca aaactatcaa tagtattcaa aaataaacca ctcataacga tactttcagt
      961 ttcaatagtt tcgttcttcg taactctacg ggctttatca ttatcatgga tctttaacat
     1021 tgtttttctg gtttcaacca tagaacctcc cggcattaat tttttcataa actgggagta
     1081 ttcgtcttct atccagaaaa tcggctgtat tggttttccg aaattatcaa ttttcttttc
     1141 tgcttttgct aactctgaaa tgaatgctgc cgaaccgtca gcaccttcaa aattaggtct
     1201 aatcattggc ttttcttcta tatcttttgg tatagaactt tcaataattt ttgctgatgt
     1261 ggtttttgct gcaccagaag gtgcaagtat taaagtccaa gtgtttaagt aatccgctgg
     1321 gcttgtagga tgcttataca ttatattgtt attaacacta aaagcactta aatagcttaa
     1381 aaatgaaaat atcggtaaag ctgggggtat gtcggtttca ctaacaaatt tagaatatat
     1441 caaagataat ggatctatat tttctgataa acctaaatta tgggggtttg gtattatagg
     1501 taaaacatca ataaaattta tattcacttc gtgagtttct acttttggct ctttgctaat
     1561 tgcttctgct atttcgttcg ttaaaactat ttcttcttct gttaaattaa ataccttttt
     1621 caatcttatc ataattaaat cctcctatta tatatacagt tataacatag gattttaact
     1681 tgtcaaatgt ttacattaaa aaaatcagac attttttatt ttgtcggatt ttaaccccaa
     1741 aaaatgcact gccaaaaaca taccaccccc cccctgtttg acactctgac attctgacaa
     1801 atcttgtaaa ctattgattt taaaggatta aaaaaaaatt ttgtcagaga tttgtcagaa
     1861 aaaatcagta ctcttttgac aaagctaatg acaaaaaatg taatatattg attataaaag
     1921 gaaaattaac ttgtcagaat gtcagaactt ttctgacaaa atggatttta aagaaaacag
     1981 gcaattttca gccctcattt gtcagaaaat atcatgtttt ttgttttgac atttgttgtc
     2041 atggtttttc tgcaaaacat atgacgtttt cttttgtggt ttttatgaca ctatgacacc
     2101 ttatgacaga aaaccgtcct accggacagg tcaacccctc acaagcaaga accagcctga
     2161 cggctggctc tcacttgttc acggttaaag ttttatcata gtgatctagt agtgttgtca
     2221 tcatattttt aatatggttt atatcgtctt ctgttgtaac ttccctgatt tttgattttg
     2281 agtttttctt cttcgatttt tcgtcttttt tcgaattttt gcctgtgttc attcttcctt
     2341 gtatatgctg aaaacgttcg tatgtatctt ccaaaagtag aaataattga gtgtacagat
     2401 cttctacata ctccaaatct tccttcagtt gtatcaatcg actttctgaa ttgctgttgt
     2461 tttcttccag cttcttcaag tctcgaacta acaaattgtt gttgctgatt aattttttca
     2521 atatctcttg ctcttgtagt tcctgctgtt tcttctgttg ctgttttagt tttatattct
     2581 gtattaattg actcattgtt tacaatctcc atattatcag tgcgaatata catacttgaa
     2641 aaaccatcgt tattactaac attgataaaa tcgccgttag tatatttata tccaatatct
     2701 tgatcttctc tttgaatact ttgtctattt tctgatatat ttttgaacga tggtttttta
     2761 aactttgatc tatttctgat ttctcgtttt tcaattcttt ctgcaaatgt tcttttaagc
     2821 tctgtgagtt tttctctaac aaactctgga tctcttgtag gttttgggtt gaattctttt
     2881 ctataatcgc tgtaatctct tccctcttca tatatatctc ctttcaggac aatgggttta
     2941 tcgtcatcaa acaaatcaga tttaattgat atttgatttt ttcgtattct gtttatttta
     3001 acacctttca tgttttcaat atgtttgatg gtttgatctc tattttccaa ctcttcatta
     3061 ataattaatg ccattatttc agtattaata ttttcaataa attgtttttt atcttctgga
     3121 atattggttt ttggtttgtt ggttaatgtg tttttatttt ctttctcttc aaatggactt
     3181 gataaattat tttcataatt aattatttca gagtagttat ttaccaaccc acgatctctt
     3241 ttatctaaat atacagtgta tgatcttctg tcttgtaaac tgttaaatgt taaataattt
     3301 aattctaccc tgcccttgtc gttgtgttct acccacataa ctggaggtct gttttcttgt
     3361 tcaatccctg caaataaaat atcttcaaac tctgtgatta atttggtttt taattcttct
     3421 gatatggatt tttcttcaaa tgataaaaca ccacacacgt atgggtttga tttgttttta
     3481 tctaaaatat aattttcaaa attgattagg tcttgttttg tggttgttga taatattttt
     3541 atgtctttct gttgttctgg gtttttctct ctcaataaat attcaatact tttttttata
     3601 ttcctttgac ctttgctttt tttacctcct attattttat gatacatatt aaattctcct
     3661 tttaatatct tctatctcat ttgttatttc ttcgaataaa tttataaaat cttctgcaaa
     3721 ctcatgggaa ataaatttat caatatcagt atttattgct cttgctatct ggttaatatt
     3781 aacacctatt ttatgaagct gataatacac atcaccattt tcatttttgg aaatttcttt
     3841 tttgagttta aactctttat tgagtattaa ctcccttatg gctttcgccc ttggcttttt
     3901 cagcatagaa gccagttctt caacctgtgc aaattcttcg tcattgaagc atagcgtgac
     3961 gctgtgcttc ctatggtatc tatccttgta gctcactgta agcctcctgt gttcggtttt
     4021 aaggggattg aagggg
//
