LOCUS       NZ_CP073001             5692 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP1878 plasmid p1878-5k, complete
            sequence.
ACCESSION   NZ_CP073001
VERSION     NZ_CP073001.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18696713
            Assembly: GCF_018075325.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Zhang,X.
  TITLE     Expansion of acquired 16S rRNA methytransferases rmtB along with KPC
            within Klebsiella Pneumoniae Complex isolated from a Chinese
            Teaching Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5692)
  AUTHORS   Zhang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2021) School of Laboratory Medicine and Life
            Science/Institute of Biomedical Informatics, Wenzhou Medical
            University, chashan, Wenzhou, Zhejiang 325000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.14.1; Canu v. 1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 08:36:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,869
            CDSs (total)                      :: 5,748
            Genes (coding)                    :: 5,575
            CDSs (with protein)               :: 5,575
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 67 of 173
            Pseudo Genes (incomplete)         :: 112 of 173
            Pseudo Genes (internal stop)      :: 26 of 173
            Pseudo Genes (multiple problems)  :: 32 of 173
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP1878"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p1878-5k"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2015/2017"
     gene            <2..292
                     /locus_tag="KA532_RS29090"
                     /old_locus_tag="KA532_29085"
                     /pseudo=""
     CDS             <2..292
                     /locus_tag="KA532_RS29090"
                     /old_locus_tag="KA532_29085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /name="antirestriction protein"
     gene            420..575
                     /locus_tag="KA532_RS29095"
                     /old_locus_tag="KA532_29090"
     CDS             420..575
                     /locus_tag="KA532_RS29095"
                     /old_locus_tag="KA532_29090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_013213988.1"
                     /translation="MNTDIVSLAKHIGDALTARRPVHMPRMKMRELGLLLIELKAYREA
                     AATVTH"
                     /name="hypothetical protein"
     gene            621..917
                     /locus_tag="KA532_RS29100"
                     /old_locus_tag="KA532_29095"
     CDS             621..917
                     /locus_tag="KA532_RS29100"
                     /old_locus_tag="KA532_29095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="WP_013213987.1"
                     /translation="MIRPETLRPFAEDWQAPTADEIKEVLELIRQRKGLSKPLSGVDVA
                     DLVGLPGERGSGKGTRTFRRWVSKTNPSPIAYGAWSILAHLAGFGAIWDADRD"
                     /name="transcriptional regulator"
     gene            <1021..1902
                     /locus_tag="KA532_RS29105"
                     /old_locus_tag="KA532_29100"
                     /pseudo=""
     CDS             <1021..1902
                     /locus_tag="KA532_RS29105"
                     /old_locus_tag="KA532_29100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152397.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISKpn6 family transposase"
                     /name="IS1182-like element ISKpn6 family transposase"
     gene            complement(2152..3033)
                     /locus_tag="KA532_RS29110"
                     /old_locus_tag="KA532_29105"
     CDS             complement(2152..3033)
                     /locus_tag="KA532_RS29110"
                     /old_locus_tag="KA532_29105"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /protein_id="WP_004199234.1"
                     /translation="MSLYRRLVLLSCLSWPLAGFSATALTNLVAEPFAKLEQDFGGSIG
                     VYAMDTGSGATVSYRAEERFPLCSSFKGFLAAAVLARSQQQAGLLDTPIRYGKNALVPW
                     SPISEKYLTTGMTVAELSAAAVQYSDNAAANLLLKELGGPAGLTAFMRSIGDTTFRLDR
                     WELELNSAIPGDARDTSSPRAVTESLQKLTLGSALAAPQRQQFVDWLKGNTTGNHRIRA
                     AVPADWAVGDKTGTCGVYGTANDYAVVWPTGRAPIVLAVYTRAPNKDDKHSEAVIAAAA
                     RLALEGLGVNGQ"
                     /name="carbapenem-hydrolyzing class A beta-lactamase KPC-2"
     gene            complement(3309..4289)
                     /locus_tag="KA532_RS29115"
                     /old_locus_tag="KA532_29110"
     CDS             complement(3309..4289)
                     /locus_tag="KA532_RS29115"
                     /old_locus_tag="KA532_29110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213985.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481-like element ISKpn27 family transposase"
                     /protein_id="WP_013213985.1"
                     /translation="MTQALHSQARTTHLIREEIRNSTLPQAELARMYNVTRQTIRKWQN
                     RESPEDKSHAPNKMYTTLTPEQELIVVELRKTLLLPTDDLLAVTREFINPAVSRAGLGR
                     CLRRHGVSDLRNLVEQEGTAPATKKTFKDYEPGFVHIDIKYLPQMPDETARRYLFVAID
                     RATRWVFIELYADQTDGSSGDFLNKVQQACPVKIVKLLTDNGSQFTDRFTAGGKKKEPS
                     GTHVFDRLCKQLGIEHRLIPPRHPQTNGMVERFNGRISDIVNQTRFGSAAELESTLRNY
                     VKIYNHSIPQRALQHKTPVQALKEWHEKRPELFRKRVYNQPGLDI"
                     /name="IS481-like element ISKpn27 family transposase"
     gene            complement(4412..>4873)
                     /locus_tag="KA532_RS29120"
                     /old_locus_tag="KA532_29115"
                     /pseudo=""
     CDS             complement(4412..>4873)
                     /locus_tag="KA532_RS29120"
                     /old_locus_tag="KA532_29115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     gene            complement(4925..5629)
                     /locus_tag="KA532_RS29125"
                     /old_locus_tag="KA532_29120"
     CDS             complement(4925..5629)
                     /locus_tag="KA532_RS29125"
                     /old_locus_tag="KA532_29120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     ARG             complement(2156..3033)
                     /name="blaKPC-2"
                     /gene="blaKPC-2"
                     /gene_name="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 ctgccgcgag tatcgcgggg cttattggct tatgtgggag ctgtcgaacg gcggctttta
       61 tatggctcct gggcgtcgtg atgaaatgct gaacatcgag gccatgaact actttagtgg
      121 ccaaatgagc gccgatgctg ccgggattac cgcttgcctc tacctctata gccatctgtc
      181 atttcacaca gaaggcgctg accaggagcg gttttcaagg ctgtatcaca gcttgcggga
      241 ttgggcttgt gagcatgacg aaaaagaggc catcttggcc gcaatagact agcaacagaa
      301 agccccggtg atggccgggg ctcttaaagg aggtgcatag gtagaaaaag aaatggtgcc
      361 cctggtatgg ctgcaaccaa tcacaggggc tttcaccggt caatttgagg ataaacgcga
      421 tgaatacgga tattgtatca cttgcaaaac acattggcga cgctctgacg gcccgccgcc
      481 ccgttcatat gccgcgcatg aagatgcggg agcttggctt gctcttgatt gaactgaagg
      541 cgtatcggga ggccgccgcg accgttaccc attgataaaa aagccccggc attgaccggg
      601 gctatttgag gatttatcgc atgattagac ctgaaacgct taggccgttc gctgaagatt
      661 ggcaggctcc aaccgccgat gaaatcaaag aggtgttgga gctgattagg caacgaaaag
      721 ggcttagtaa gcctctcagt ggcgttgatg tcgctgatct cgttggtttg cctggtgaga
      781 gaggcagcgg aaagggaacc cgaaccttca ggcgttgggt gagcaaaacc aaccctagcc
      841 ctattgctta tggtgcctgg tctatcttgg cccacctggc aggtttcggg gctatatggg
      901 acgctgaccg ggattgaaac catgaccgaa cataaggccg aacgtgctcc ctggggagac
      961 ttcccggcag tagtgaggaa cggcgatcta aaggatctct ctaaagaacc tgaatacgaa
     1021 gccgccaaac atggcgacca caaggcgatg agctataagc gcatgaagcc ggccgaggac
     1081 gaattgcatt gcgagatcaa ggcgctgctt gatcgcgcca aggctaccga cgaccaggag
     1141 cgtaacgagc cggagctgga cattcctgcc gagatttctc gccgcgagaa gcgcctggag
     1201 gcgatccagg cggcaaaggc gcgcctggaa gcgcgccagc gtgaagcgga ccaggcccgg
     1261 gggcgcagcg aagacgatgg ccgccggcct cgccatccgg atggctcgga caagggcggt
     1321 ggctcgtaca aacgcgagtt tggtgtgccg gatgaccgtg atcaggaaag cttcaccgat
     1381 ccggacagcc ggatcatgaa acacgccggt ggtggctccg agcagagcta caacgggtac
     1441 acagcggtcg atgccgagca ccagatcatc gtggcggcgg agttgaccaa ctgcgccgcg
     1501 gacagtcagg cgctgctggg catgctggca gcagttcagg ccaacaccgg agaaatgccg
     1561 gcccagacgc tggcggatgc gggattccgt agtgaggctg ttctggcaaa ggtcgccgat
     1621 caccacggcg atgtcatcgt tgccctcggc cgcgagggac gtgaagatgc caaggtcaat
     1681 gccaagaccc atccgcatac ggcggcgatt gcggcgaaat tgaaaacgga gcaaggcgat
     1741 gcagcttacc gccggcgcaa gtcgatcgtg gaggctccga atggttggat caaggcggtg
     1801 atgggattgc gtcagttcag catgaggggc ctggacaagg tgcaagccga gtggaagctc
     1861 gtctgcatgg cgctaaatct gaggcgaatg gcgtatctgt gagggcgaag gttaaatggg
     1921 gcggctcaaa tgcaccccag tcgtcataac acgccgcgcg ccgcaagatt ggtatccttg
     1981 gcgccgacgc cttgccaatt gcagagagcg ccgccgccat cgtcagtgct ctacagaaaa
     2041 ccggtcacac ggctctgccg cgcagactcc tagcctaaat gtgacagtgg ttggtaatcc
     2101 atgccgcccg caagggcggc ggtggtgggc caatagatga ttttcagagc cttactgccc
     2161 gttgacgccc aatccctcga gcgcgagtct agccgcagcg gcgatgacgg cctcgctgtg
     2221 cttgtcatcc ttgttaggcg cccgggtgta gacggccaac acaataggtg cgcgcccagt
     2281 gggccagacg acggcatagt catttgccgt gccatacact ccgcaggttc cggttttgtc
     2341 tccgactgcc cagtctgccg gcaccgccgc gcggatgcgg tggttgccgg tcgtgtttcc
     2401 ctttagccaa tcaacaaact gctgccgctg cggcgcagcc agtgcagagc ccagtgtcag
     2461 tttttgtaag ctttccgtca cggcgcgcgg cgatgaggta tcgcgcgcat cgcctgggat
     2521 ggcggagttc agctccagct cccagcggtc cagacggaac gtggtatcgc cgatagagcg
     2581 catgaaggcc gtcagcccgg ccgggccgcc caactccttc agcaacaaat tggcggcggc
     2641 gttatcactg tattgcacgg cggccgcgga cagctccgcc accgtcatgc ctgttgtcag
     2701 atatttttcc gagatgggtg accacggaac cagcgcattt ttgccgtaac ggatgggtgt
     2761 gtccagcaag ccggcctgct gctggctgcg agccagcaca gcggcagcaa gaaagccctt
     2821 gaatgagctg cacagtggga agcgctcctc agcgcggtaa cttacagttg cgcctgagcc
     2881 ggtatccatc gcgtacacac cgatggagcc gccaaagtcc tgttcgagtt tagcgaatgg
     2941 ttccgcgacg aggttggtca gcgcggtggc agaaaagcca gccagcggcc atgagagaca
     3001 agacagcaga actagacggc gatacagtga catcaacgat attccttgtt tgaaggtgga
     3061 gctaggtgta gcggccggat gtaatcaggc cgctacagct gggattaggg gtctgacgct
     3121 cagtggaacg aaaactcacg ttaagaagtc atttttcaat attattgaag catttatcag
     3181 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3241 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     3301 acattaacct atatgtcaag acccggctgg ttatacacgc gtttcctgaa caattcaggg
     3361 cgtttttcat gccattcctt gagcgcctga acgggtgttt tgtgttggag cgcgcgttgc
     3421 ggaatgctgt ggttgtagat cttgacgtaa ttgcgcagcg tcgattccag ttcggcagct
     3481 gaaccaaaac gggtctggtt gacgatgtcg ctgatacgac cgttgaagcg ctccaccatg
     3541 ccgttggtct gcggatgacg aggcgggatg agccggtgct cgatgccgag ctgcttgcac
     3601 aggcggtcga acacgtgtgt gccgctgggt tccttcttct tgccgccagc cgtgaagcgg
     3661 tcggtgaact ggctgccgtt gtcggtcaga agcttgacga tcttgacggg acaggcttgc
     3721 tggactttgt tgaggaagtc gccactgctg ccatcggtct ggtcggcata gagctcgatg
     3781 aagacccagc gcgtagcacg gtcgatggca acgaagagat agcgccttgc cgtctcgtcg
     3841 ggcatctgcg gcaggtactt gatgtcgatg tgcacaaagc ccggctcgta gtccttgaag
     3901 gtctttttcg tggcgggcgc agtgccttcc tgctcgacca ggttacgtag atccgagaca
     3961 ccgtggcggc gcaggcaacg tcccaggccg gcacgcgaga cggctggatt gatgaactcg
     4021 cgcgtgaccg ccagcaggtc gtccgtgggc agcagcaacg tcttgcgcag ctccaccacg
     4081 atgagctcct gctcgggcgt gagcgtcgtg tacatcttgt tcggcgcatg cgacttgtct
     4141 tcaggagact cgcggttttg ccactttcgg atggtttggc gggtgacgtt gtacatcctg
     4201 gccagttcgg cctgcgggag cgtcgagttc ctgatttcct cacggatcag gtgggtagta
     4261 cgggcttggc tgtgcagtgc ctgggtcatc gagttgccag tgaagagtcg gattggaaca
     4321 gtgtacgcaa aacgtgtctg gccttgaaga gcggccagct acgcatgggt acatgatccc
     4381 acgagtccag acacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaattt
     4441 tataaaccgt ggagcgggca atactgagct gatgagcaat ttccgttgca ccagtgccct
     4501 tctgatgaag cgtcagcacg acgttcctgt ccacggtacg cctgcggcca aatttgattc
     4561 ctttcagctt tgcttcctgt cggccctcat tcgtgcgctc taggatcctc cggcgttcag
     4621 cttgtgccac agccgacagg atggtgacca ccatttgccc catatcaccg tcggtactga
     4681 tcccgtcgtc aataaaccga accgcgacac cctgagcatc aaactctttt atcagttgga
     4741 tcatgtcggc ggtgtcgcgg ccaagacggt cgagcttctt caccagaatg acatcacctt
     4801 cctccacctt catcctcagc aaatccagcc cttcccgatc tgttgaactg ccggatgcct
     4861 tgtcggtaaa gaggcactgt tgcaaagtta gcgatgaggc agccttttgt cttattcaaa
     4921 ggccttacat ttcaaaaact ctgcttacca ggcgcatttc gcccagggga tcaccataat
     4981 aaaatgctga ggcctggcct ttgcgtagtg cacgcatcac ctcaatacct ttgatggtgg
     5041 cgtaagccgt cttcatggat ttaaatccca gcgtggcgcc gattatccgt ttcagtttgc
     5101 catgatcgca ttcaatcacg ttgttccggt acttaatctg tcggtgttca acgtcagacg
     5161 ggcaccggcc ttcgcgtttg agcagagcaa gcgcgcgacc ataggcgggc gctttatccg
     5221 tgttgatgaa tcgcgggatc tgccacttct tcacgttgtt gaggatttta cccagaaacc
     5281 ggtatgcagc tttgctgtta cgacgggagg agagataaaa atcgacagtg cggccccggc
     5341 tgtcgacggc ccggtacaga tacgcccagc ggccattgac cttcacgtag gtttcatcca
     5401 tgtgccacgg gcaaagatcg gaagggttac gccagtacca gcgcagccgt ttttccattt
     5461 caggcgcata acgctgaacc cagcggtaaa tcgtggagtg atcgacattc actccgcgtt
     5521 cagccagcat ctcctgcagc tcacggtaac tgatgccgta tttgcagtac cagcgtacgg
     5581 cccacagaat gatgtcacgc tgaaaatgcc ggcctttgaa tgggttcatg tgcagctcca
     5641 tcagcaaaag gggatgataa gtttatcacc accgactatt tgcaacagtg cc
//
