LOCUS       NZ_CP073000             6796 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP1878 plasmid p1878-6k, complete
            sequence.
ACCESSION   NZ_CP073000
VERSION     NZ_CP073000.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18696713
            Assembly: GCF_018075325.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Zhang,X.
  TITLE     Expansion of acquired 16S rRNA methytransferases rmtB along with KPC
            within Klebsiella Pneumoniae Complex isolated from a Chinese
            Teaching Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   Zhang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2021) School of Laboratory Medicine and Life
            Science/Institute of Biomedical Informatics, Wenzhou Medical
            University, chashan, Wenzhou, Zhejiang 325000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.14.1; Canu v. 1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 08:36:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,869
            CDSs (total)                      :: 5,748
            Genes (coding)                    :: 5,575
            CDSs (with protein)               :: 5,575
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 67 of 173
            Pseudo Genes (incomplete)         :: 112 of 173
            Pseudo Genes (internal stop)      :: 26 of 173
            Pseudo Genes (multiple problems)  :: 32 of 173
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073000.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP1878"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p1878-6k"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2015/2017"
     gene            complement(join(6774..6796,1..349))
                     /locus_tag="KA532_RS29030"
                     /old_locus_tag="KA532_29025"
     CDS             complement(join(6774..6796,1..349))
                     /locus_tag="KA532_RS29030"
                     /old_locus_tag="KA532_29025"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228264514.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLRKVRTSS"
                     /name="hypothetical protein"
     gene            complement(375..701)
                     /locus_tag="KA532_RS29035"
                     /old_locus_tag="KA532_29030"
     CDS             complement(375..701)
                     /locus_tag="KA532_RS29035"
                     /old_locus_tag="KA532_29030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(1059..1217)
                     /locus_tag="KA532_RS29665"
     CDS             complement(1059..1217)
                     /locus_tag="KA532_RS29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1857..2033
                     /locus_tag="KA532_RS29040"
                     /old_locus_tag="KA532_29035"
     CDS             1857..2033
                     /locus_tag="KA532_RS29040"
                     /old_locus_tag="KA532_29035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            2023..2367
                     /locus_tag="KA532_RS29045"
                     /old_locus_tag="KA532_29040"
     CDS             2023..2367
                     /locus_tag="KA532_RS29045"
                     /old_locus_tag="KA532_29040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2576..>3010
                     /locus_tag="KA532_RS29545"
                     /pseudo=""
     CDS             2576..>3010
                     /locus_tag="KA532_RS29545"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3045..3554
                     /locus_tag="KA532_RS29055"
                     /old_locus_tag="KA532_29050"
     CDS             3045..3554
                     /locus_tag="KA532_RS29055"
                     /old_locus_tag="KA532_29050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            3561..3773
                     /locus_tag="KA532_RS29060"
                     /old_locus_tag="KA532_29055"
     CDS             3561..3773
                     /locus_tag="KA532_RS29060"
                     /old_locus_tag="KA532_29055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4440..4664
                     /locus_tag="KA532_RS29065"
                     /old_locus_tag="KA532_29060"
     CDS             4440..4664
                     /locus_tag="KA532_RS29065"
                     /old_locus_tag="KA532_29060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            4648..4914
                     /locus_tag="KA532_RS29070"
                     /old_locus_tag="KA532_29065"
     CDS             4648..4914
                     /locus_tag="KA532_RS29070"
                     /old_locus_tag="KA532_29065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4984..5334)
                     /locus_tag="KA532_RS29075"
                     /old_locus_tag="KA532_29070"
     CDS             complement(4984..5334)
                     /locus_tag="KA532_RS29075"
                     /old_locus_tag="KA532_29070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(5400..5588)
                     /locus_tag="KA532_RS29080"
                     /old_locus_tag="KA532_29075"
     CDS             complement(5400..5588)
                     /locus_tag="KA532_RS29080"
                     /old_locus_tag="KA532_29075"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228264513.1"
                     /translation="MSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDNF
                     RLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(5578..5766)
                     /locus_tag="KA532_RS29670"
     CDS             complement(5578..5766)
                     /locus_tag="KA532_RS29670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_323129225.1"
                     /translation="MGEISVKTGNNARNGGKNSLNRLIRCVCGKKIGPDPRNFNQRVSL
                     GRNDLLIRTFPNIYNEH"
                     /name="hypothetical protein"
     gene            complement(5748..6728)
                     /locus_tag="KA532_RS29085"
                     /old_locus_tag="KA532_29080"
     CDS             complement(5748..6728)
                     /locus_tag="KA532_RS29085"
                     /old_locus_tag="KA532_29080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="WP_000019473.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMVEVIEPFY
                     PKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLIAERPGKVKTLKQHPRKNKTAINIEYMKASIRARVEHPFRI
                     IKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSQ"
                     /name="IS5-like element ISKpn26 family transposase"
     Typing          1692..1771
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(1109..1506)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          2358..3889
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttagtggatt atcatttttc ttcccccatg ggtttctaat taatccttcg atgtactcag
       61 gtcctttgcg tcctattgcc tgcgcaatat tttttatttc atctgcgtga gggctttgtt
      121 gtacagcttt gtttatgcct gcttgagtga ttttcagaga ctctacaccg tgtatgttgt
      181 atgagataaa atgtaataaa ttaagatgca atgcaattaa tccatttttg tttacatctg
      241 agtatttgga taaaaaacgt aatgaagtgt taactctccc ttgaagccta acaattggga
      301 gtgctgcctt gtatatttcc ttctccgtta aataccgttt aaaaaacata attctccgcc
      361 tccgtatagc gtgcctattt tatttcgtcg taaaacttag ctctgaaata ttcctttaga
      421 accatcatgt cattaataat tgaatctatc tcatccattt tttcttggta ttcttttaat
      481 ggtgaaggga gcggtaactc aatatttgaa aagtgattgg cgagataggg atcaaggttt
      541 ctcatccatt caggcttttc gccaatgcgt tcttcaactg aaagtgaacc tgataatctc
      601 tggttaataa gatcacttag actcattttg gttttattcg aaagccgttg taaggattgc
      661 tttaatccta tatctagcat gatatttgtt ctttctctca ttaccccccc ttgctattgc
      721 gttttttgtt gcgcacaaag ctaccattgc gcattaagaa acgcaataaa aaaggaggga
      781 gggagtggtt tgtacatgat gtgcagtgcg ctgagggcat cagtggcggg tgtttttccg
      841 tgggatggat ggagctccgg ggataacaga actgacgatc atttaaagga tcttcttgag
      901 atcctttttt tctgcgcgta atctcttgca ctgtaaacga aaaaaccgcc tggggaggcg
      961 gtttgatcga aggttaagtc agttggggaa ctgcttaacc gggtaactgg gtatacgaga
     1021 ccgcagatac caaaactgtt ctttcagtgt agccgcagtt aggcacccac ttcaagacct
     1081 ctcaatatct ctctcgtata tatccagtta ccaatggctg ctgccagtgg cgtttttgcg
     1141 tgtccgtccg ggttggactc aagatgatag ttaccggaaa gggcgcagca gtcgggctga
     1201 acggggggtt cgtgcataca gcccagcttg gagcgaacga cctaaaccga gccgagatac
     1261 cagcagcgtg agctatgaga aagcgccacg cttcccgaaa gggaaaaagg cggaacaggt
     1321 atccggtaaa cggcagggtc ggaacaggag agcgcacgag ggagccgccg gagagaaatc
     1381 accggcatct ttaagtcctg tcgggtttcg tcctcctctg acttgagcgt ccatttctgt
     1441 gatgctcgtc aggggaggcg gagcctatgg aaaaacggca acgccgcatc cttttctccg
     1501 gccctttctg atttgagcgc ctttgcttta tcccgttatc cgtgagtcgg tcacgcccgc
     1561 cgcagccgaa cggcaggagc gcagctaccg agtgagcgag gaggcgtcat ttcatctgtc
     1621 cgtgcatggt attgcggacg ctcctgagcc acatatcaca cctgccacat gaggcacttt
     1681 catgaatcgt gctctcatgc caacatagta agccagtata cacagcgtaa acgctgtgac
     1741 tggttcaggg ctgcgccccg aaacccgcta acaccactga cgcgctggcg cttgtccaac
     1801 gccggagcga cccggaaaac ttttccgtgc cgtccggcgt ttgcgaagga gggataatgc
     1861 agaagccgaa gaagaagccg ctcgtcgtaa aaaaaagatc ctggcatccg gcagtgatgg
     1921 caaatctgat gcgtcaggca caggaggaag attaccgccg ggagcagatg gcagagcttg
     1981 agcgttcagc ccggtcacat tttgcggata tcggaggaga ggatggcggt taagcgtacc
     2041 cgttttttag gtatccgggt gaccgatggg gagtaccagc agctgctgga gcgctgcaat
     2101 ggaaggcaac tggcggtgtg gatgcgggaa acctgtctcg atacgcgccc ggcgcggtcg
     2161 ttgcggctgc cgtccatcga tccggttttg ctgcgccagc ttgccgggat ggggaacaac
     2221 ctcaaccaga tcgcccggaa aattaacggc ggccagtggt caggcgctga ccgggttcag
     2281 gtggtggccg cgctgatggc cattgatgcc gaactcgagc ggctgcggca tgccgtgctg
     2341 gaaaaggggg cagacgatga tcgttaagtt tcatccgcga gggcgaggcg gcggtgccgg
     2401 gccggtggat tatctgctgg ggaaagaccg ccagcgcgac ggggccatcg tcctgcaggg
     2461 aaagccggag gaggtccggg agcttatcga cgcctcgccc tacgccaaaa agtacacctc
     2521 cggcgtcctg tcttttgctg aacaggattt accgcccggc cagcgtgaaa agctgatggc
     2581 gagcttcgag cgggttctga tgcccggact cgataaagac cagtacagcg tgctgtgggt
     2641 tgaacaccgg gacaaggggc ggctggaact gaattttctg gtaccgaaca cggaactgct
     2701 gaccggcagg cgtctccagc cgtactacga ccgggcagac cgtccgcgca tcgatgcgtg
     2761 gcagaccgtg gtgaacgggc gactggggct gcacgacccg aacgcaccgg agaaccggcg
     2821 cgcgctggtg acgccatctt cgttacccaa aacgaagcag gaagccgcag aggctattac
     2881 gcggggctta ctggccctcg cctcgtcagg ggagcttaaa acgcgtcagg acgtcactga
     2941 ggcgctggaa agcgcaggtt ttgaggtcgt gcgcaccaca aagagcagca tcagcattgc
     3001 cgacccggac ggagggcgaa acatccgact taagggagcc atctatgaac agtcttttaa
     3061 cgctggcgaa ggacttagag cagaaatcga aagcgcagca gcagagtacc ggcgaaatgc
     3121 tgaaagccgc attcagcgag cacgagaagt ctgtcagagc ggaactgagc gaaagcggga
     3181 agagaatcag cgccgccatc cgcgcccacg agcagggtat gagcgcggcc atgcagtcga
     3241 accgcctgag cgtgatgcgt atggtcgggc ggacatggct gaccatcacg atggtgtcag
     3301 gactgctgtt cgccagcctg agcggggtgt tgtggtatca ggggagcttg atagcgtcaa
     3361 atctggccga aatcgaccgg cagaacgcag cgctgtcgaa gctgaacgcg aagacctggg
     3421 gcgtgacgta cctggaagac agcaacggac gttttctggt gctgccgaag gggacggccg
     3481 tagacaggac gcagagctgg acggtcggga acgggcggtc gaagcagaac gcgctgagac
     3541 tggtgaagga gtaagtgccg atgaccgagc tggaaaaaca gttgctgagc gcattagagc
     3601 agctacagca ggactactcg aaaaggctgg acgagtggga gagcgccttc ggggaatggc
     3661 ggaaaatgtc agggcttatg caacgggaga acgcggcgct gagcgagcgc gtgacgcgct
     3721 tgagtcagca ggtggaacgc ttgagcgggc aactgcagcg tttgagccgg tagtgcagcg
     3781 tcatgagatg gccgtggccg cagaacgggc gcacgagcaa cggcagcacg aaaaggaact
     3841 gacggaagcg cgttcccggc agcgaagcta tgacggtccg tcgctgggct gaaatgcagg
     3901 aaaacagggt tctcatcggg catttacgct gaaaccggat gtcattcacc tggctgcatg
     3961 gctatgcagc caggtaaaat ttatctgtcg cgaacggggc ggtttatcgg gtggtttgtt
     4021 gccggtttta cctgactgcc accccgttcg cggcgaaccc gtccggggcg gtgcgggcaa
     4081 cgggtgttat gtttaatcag cgtgaacggc agatacaaaa aagccccgga gggcttttaa
     4141 ctactgactt atttttttga agatttgctg ggtttcaatt tgtcagtatt tcccggcgtt
     4201 tttttattgt cacgctggcg tttatcaggt tcccctgttg aatcggcaat tcgtttcgga
     4261 cccggcttaa gacccataat cgttatcctt tcaatagtta agaggaaagc cctcaagcta
     4321 gatatattga ccgcgttacg gattatcaag aggggctaac aagattattt tttgttcttt
     4381 taggttgatt gtggtactgt tgatatacat gtatagcaaa tggtgttcgg gaggtttata
     4441 tgcttgctat ccggttatct gatgagattg agtcccgtct ggactcgctg gcgaagcaaa
     4501 ccggcagaac aaagacgttt tatgcgcggg aagcaatact ggcgcatctg gaagacctgg
     4561 aggattatta tctttcagca gaaactgctg cacgcgttcg ccgtggtgat gaagcagtgc
     4621 attcgtctga agacgtgagg aagtcacttg gtctggacga ttaactattc cgatcgggcg
     4681 ctcaaatcgt tacgcaagat ggacaaacag aacgcacgac ggattgtgga ttttatgagt
     4741 ttacgcattg cagttgctgc cgatcctcgc cagtcaggga agccgctcaa aggtgagctg
     4801 ggcgagttct ggcgctatcg ggtgggagat tatcgcgttc tgtgtgagat ccgagatgac
     4861 gagcttgtta tccttgccgc cacgattgga catcgccgcg aagtttacga ctgaagcccg
     4921 ctatgcgggc ttttttgatg ccgacaacag gtattatggt aaatactcga ctaactacgt
     4981 gttctactgt ttgtcggttt tagttaacca gcaggttgtc ttaacgataa tgtagccgtt
     5041 atttttaact aataaggtca tattgttaac gtcaggcata ataaaagaca ttatggcctc
     5101 agagttatca tcagaagcag ctctctgcat tactacttcg attccctttt ctttgaatct
     5161 ataagtatcc agttgcctat taaacttaga aagacctaca accatactta gtttgttatc
     5221 tttaacagta agagatgctt cctgagtacc attacatcgg tagtaagcgg tgtaaccgtc
     5281 agcaaaagat cgaccgggga atataaatgc taggaacgca attaaccaat acatagttga
     5341 gaatcttggt attacacatt tcactatcct ctctttcatt tttcgttcac catactttgt
     5401 tatctgattt ttaatttttc tattttataa accaagtctc taccaaaaag gcgaaaatta
     5461 tcctcaaata ataataattg tttaagaaca tccatgaaag cagctgcttc accctcagtt
     5521 atgggagcta ctctatataa catataacct gagttaaggg tttcttttat actttcttca
     5581 atgctcatta taaatattag ggaaggtgcg aataagcagg tcatttcttc ccaagctgac
     5641 tcgctgatta aaatttcgcg gatctgggcc gatttttttc ccgcaaacac atcgaatcag
     5701 cctatttagg ctattttttc caccatttct ggcgttattt ccggttttta ctgagatctc
     5761 tcccactgac gtatcatttg gtccacccga aacaggttgg ccagggtgaa taacatcgcc
     5821 agttggttat cgtttttcag cagccctttg tatctggctt tcacgaagcc gaactgccgc
     5881 ttgatgatgc gaaacgggtg ctccaccctg gcacggatgc tggctttcat gtattcgatg
     5941 ttgatggccg ttttgttctt gcgcggatgc tgcttcaagg tttttacctt gccgggacgc
     6001 tcggcgatca gccagtccac atccacctcg gccagctcct cgcgctgtgg cgctccttgg
     6061 tagccggcat cggctgagac aaattgctcc tctccatgaa gcagattacc cagctgattg
     6121 aggtcatgct cgttggccgc ggtggtgact aggctgtggg tcaggccact cttggcatcg
     6181 acaccaatgt gggccttcat gccaaagtgc cactgattgc ctttcttggt ctgatgcatc
     6241 tccggatcgc gttgctgctc tttgttcttg gtagagctgg gtgcctcaat gatggtggca
     6301 tccaccaaag tgccttgggt catcatgacg cctgcttcgg ccagccagcg attgatggtc
     6361 ttgaacaatt gacgggccag ttgatgctgc tcgagcaggt ggcggaaatt catgatggtg
     6421 gtgcgatccg gcagggcgct atccagggat aatcgggcaa acaggcgcat ggaggcgatt
     6481 tcgtacaggg catcttccat ggcaccgtcg ctcaggttgt accaatgctg catgcagtga
     6541 atacgcagca tggtctccag cggatagggc cgtcggccat tgcccgcctt gggataaaac
     6601 ggctcgatga cttccaccat gttttgccat ggcagaatct gctccatgcg ggagaggaaa
     6661 atctcttttc gggtctgacg gcgcttagtg ctgaattcac tatcggcgaa ggtgagttga
     6721 tggctcatga tgtccctctg ggatgcgctc cggatgaata tgatgatctc atatcaggaa
     6781 cttgttcgca ccttcc
//
