LOCUS       NZ_CP072999             9111 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP1878 plasmid p1878-9k, complete
            sequence.
ACCESSION   NZ_CP072999
VERSION     NZ_CP072999.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18696713
            Assembly: GCF_018075325.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9111)
  AUTHORS   Zhang,X.
  TITLE     Expansion of acquired 16S rRNA methytransferases rmtB along with KPC
            within Klebsiella Pneumoniae Complex isolated from a Chinese
            Teaching Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9111)
  AUTHORS   Zhang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2021) School of Laboratory Medicine and Life
            Science/Institute of Biomedical Informatics, Wenzhou Medical
            University, chashan, Wenzhou, Zhejiang 325000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.14.1; Canu v. 1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 08:36:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,869
            CDSs (total)                      :: 5,748
            Genes (coding)                    :: 5,575
            CDSs (with protein)               :: 5,575
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 67 of 173
            Pseudo Genes (incomplete)         :: 112 of 173
            Pseudo Genes (internal stop)      :: 26 of 173
            Pseudo Genes (multiple problems)  :: 32 of 173
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072999.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9111
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP1878"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p1878-9k"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2015/2017"
     gene            complement(41..985)
                     /locus_tag="KA532_RS28975"
                     /old_locus_tag="KA532_28970"
     CDS             complement(41..985)
                     /locus_tag="KA532_RS28975"
                     /old_locus_tag="KA532_28970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004150866.1"
                     /translation="MTEPTNKDSEIKKHLLDLLNSQSENIAKHFYSHIKNLIEAGEFSE
                     AHNNLALIEKYMTRPQMDEEPNINENETNKRKNVKSLEPNNYVEHIIQLEERNSILTLQ
                     LEHYTQDLNRKNAIIEKNVKQVNSLISENKELRSQVQQQRIDEKIPTYVNDVKSDLASD
                     DKHFILMSIIWSIAGVFFGFLAVISAFFTLYMNLDLKNLTNLQLIYIFTRGLVGIAILS
                     WLSYICLSNSKKYTHESIRRKDRRHALMFGQVFLQIYGSTATKEDAIEVFKDWNISGDS
                     AFSGQTEQPPSFASFLNTIKDKVKVTGSDKETD"
                     /name="hypothetical protein"
     gene            complement(997..1932)
                     /locus_tag="KA532_RS28980"
                     /old_locus_tag="KA532_28975"
     CDS             complement(997..1932)
                     /locus_tag="KA532_RS28980"
                     /old_locus_tag="KA532_28975"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein CI"
                     /protein_id="WP_004153817.1"
                     /translation="MSTHEITFRHVDEKKKSLIMQNRGGQSVIARILKAYGFSSRQAFC
                     NHLGISQSTMANRYARDTFPADWVVICSMETGVPVEWLAFGTDTEKGRITNNAEKSHNN
                     CDSKHQHLNREQDIQNENFFTINQGGKAAIERIVLAYGFKTRQALADHIGVSKSTLANR
                     YMRDTFPADWIIQCSLETGASLTWLTTGNGAMFEKPRINTITIPYHKIIDGSLAQETVL
                     TFDSKLLEGTFLQPLAVFIDEEIYIVESKFNEVTDGKWLVNIEGKISIKDLTRIPVGMV
                     KVVGTNASFECLLTDIIVLAKCKRVFTKNV"
                     /name="phage repressor protein CI"
     gene            2021..2242
                     /locus_tag="KA532_RS28985"
                     /old_locus_tag="KA532_28980"
     CDS             2021..2242
                     /locus_tag="KA532_RS28985"
                     /old_locus_tag="KA532_28980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000188448.1"
                     /translation="MTPNISITLNTPHVTIERYSELTGLSIDTINDMLADGRIPRHRLR
                     KDKKREKVMINLAALTVDALTDCNVVFN"
                     /name="hypothetical protein"
     gene            2275..2784
                     /locus_tag="KA532_RS28990"
                     /old_locus_tag="KA532_28985"
     CDS             2275..2784
                     /locus_tag="KA532_RS28990"
                     /old_locus_tag="KA532_28985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="WP_000460893.1"
                     /translation="MFDYQVSKHPHFDEACRAFALRHNLVQLAERAGMNVQILRNKLNP
                     AQPHLLTAPEIWLLTDLTEDSTLVDGFLAQIHCLPCVPINEVAKEKLPHYVMSATAEIG
                     RVAAGAVSGDVKTSAGRRDAISSINSVTRLMALAAVSLQARLQANPAMASAVDTVTGLG
                     ASFGLL"
                     /name="phage regulatory CII family protein"
     gene            2792..2992
                     /locus_tag="KA532_RS28995"
                     /old_locus_tag="KA532_28990"
     CDS             2792..2992
                     /locus_tag="KA532_RS28995"
                     /old_locus_tag="KA532_28990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage filamentation protein Fil family protein"
                     /protein_id="WP_000956179.1"
                     /translation="MLTKEPSFASLLVKQSPAMHYGHGWIMGEDGKRWHPCRSQDELLA
                     ELSTKKQGNKWLLKALRRLFH"
                     /name="phage filamentation protein Fil family protein"
     gene            2956..3297
                     /locus_tag="KA532_RS29000"
                     /old_locus_tag="KA532_28995"
     CDS             2956..3297
                     /locus_tag="KA532_RS29000"
                     /old_locus_tag="KA532_28995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5347 domain-containing protein"
                     /protein_id="WP_000963473.1"
                     /translation="MAIEGAAATVPLSPGERLNGLNHIAELRAKVFGLNIESELERFIK
                     DMRDPRDINNEQNKRALAAIFFMAKIPAERHSISINELTTDEKRELIKAMNHFRAVVSL
                     FPRRLTMPN"
                     /name="DUF5347 domain-containing protein"
     gene            3365..3598
                     /locus_tag="KA532_RS29005"
                     /old_locus_tag="KA532_29000"
     CDS             3365..3598
                     /locus_tag="KA532_RS29005"
                     /old_locus_tag="KA532_29000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="WP_004150864.1"
                     /translation="MRSIETLTTKTGPDDAGLNILLTEARLEDRRARAEAMAARLDSLA
                     CHITSRQLNHVEAAELLRVTAEAIQNEAQEIH"
                     /name="DUF2732 family protein"
     gene            3598..3825
                     /locus_tag="KA532_RS29010"
                     /old_locus_tag="KA532_29005"
     CDS             3598..3825
                     /locus_tag="KA532_RS29010"
                     /old_locus_tag="KA532_29005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="WP_000752622.1"
                     /translation="MADAMDLVQQRVEEERQRHIRTARAKTPGVSRVLCIECEAPIPPA
                     RRRAIPGVQLCITCQEIAELKGKHYNGGAV"
                     /name="TraR/DksA family transcriptional regulator"
     gene            3822..4679
                     /locus_tag="KA532_RS29015"
                     /old_locus_tag="KA532_29010"
     CDS             3822..4679
                     /locus_tag="KA532_RS29015"
                     /old_locus_tag="KA532_29010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="WP_004150863.1"
                     /translation="MSTILKWAGNKTAIMPELKKYLPAGPRLVEPFAGSCAVMIETDYP
                     SYLVADINPDLINLYKKVAADCEAFISRARALFEEANREVAYYNIRQEFNYSTEITDFM
                     KAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKEIRKFAEKAQRATFICASF
                     DETLAMLKAGDVVYCDPPYYGTFSGYHTDGFTKDDQYHLASVLEHRSSEGHPVIVSNSD
                     TSLIRSLYRNFTHHYIKAKRSIGVSAGESKSATEIIAVSGARCWVGFAPSRGVDSSAVY
                     EVRV"
                     /name="DNA adenine methylase"
     gene            4676..7090
                     /locus_tag="KA532_RS29020"
                     /old_locus_tag="KA532_29015"
     CDS             4676..7090
                     /locus_tag="KA532_RS29020"
                     /old_locus_tag="KA532_29015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="WP_004150862.1"
                     /translation="MSHDDMSNSSGFNEAAASFSWNGPKKAINPYLDPAEVAPESALSN
                     LITLYAADNEQEQLRREALSEQVWERYFFNESRDPVQREMEQDKLISRVKLAHEQQRFN
                     PDMVILADVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRDSQL
                     STSFRFMASHEGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDVKPEE
                     IRKTWEKVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWYRKLWK
                     MRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEDGDTLDME
                     DVVNASCSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNNGRPNPTW
                     KNATVRQSSDYLVDMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCFMRKKDRRA
                     ITALLRKFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNIDGRGLAGEIS
                     KETGKSLRDNAEYVNAWASLHRVQQFRFFGIPGRQAYRELRLLAGQAARQQGDKKAGVP
                     VLDNPRLDAILAAADAGCFATYIMKQGGVLVPRKYHLIRTAYEINEEPTAYGDHGIRIY
                     GIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCPLAENLNQQGKDK
                     SADGDTRTDITRMDDKELHDYLHSMSKKERRELAARLRLVKPKRRKDYKQRITDHQRQQ
                     LVYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLQEDDKWRNLY"
                     /name="replication endonuclease"
     gene            7566..9053
                     /gene="ltrA"
                     /locus_tag="KA532_RS29025"
                     /old_locus_tag="KA532_29020"
     CDS             7566..9053
                     /gene="ltrA"
                     /locus_tag="KA532_RS29025"
                     /old_locus_tag="KA532_29020"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
ORIGIN
        1 attcaatgta ttttttactt aggagcatac atgttcatga ttaatctgtt tctttatcac
       61 ttccagttac tttaactttg tctttgattg tattcaaaaa tgacgcaaag ctcggtggtt
      121 gctctgtctg acctgaaaat gcagagtcac ctgaaatatt ccaatcctta aagacttcta
      181 ttgcatcctc tttagttgct gtagaaccgt atatctgcaa aaaaacttga ccaaacatca
      241 aagcatgtcg acgatctttt cgcctgatcg attcatgtgt gtactttttt gagttactaa
      301 ggcagatata tgatagccat gaaagaatgg cgattccaac taatcctcgc gtgaagatat
      361 atattaactg aaggttagtg agatttttta aatctaagtt catgtataat gtaaaaaaag
      421 cagatattac cgcaaggaag ccaaaaaata ccccagcaat agaccagata atagacatca
      481 atataaaatg tttgtcatca ctagcaagat ctgatttaac atcgttaaca taggtgggga
      541 ttttttcatc gattctttgt tgctgtactt ggctacggag ttccttattt tcactaatca
      601 aagaattaac ttgttttacg tttttttcga ttattgcgtt ttttctatta agatcctgag
      661 tataatgctc taactgtaga gttaatatgc tgtttcgttc ttctaattgt attatatgtt
      721 ctacataatt attaggttca agtgatttta cattttttct tttattagtt tcattttcat
      781 ttatattggg ttcttcatcc atctgtggcc tagtcatgta tttttcaatt agtgctaggt
      841 tattatgagc ttcagaaaac tctcctgctt ctattaagtt ttttatatga gagtagaagt
      901 gttttgctat attttcagac tgtgaattaa gcaagtctaa taggtgtttt tttatttcgc
      961 tatctttatt ggttggttca gtcatgatgt ttctctttat acatttttag taaaaactct
     1021 tttacatttt gccaaaacga taatgtcagt aagtaagcat tcaaaacttg cgttagtgcc
     1081 tacaacttta accataccaa cgggtatgcg agtcaaatct ttgatgctta ttttcccttc
     1141 aatattcaca agccacttgc catcagtaac ttcattaaat tttgattcta caatatatat
     1201 ttcctcatca atgaatactg ctaaaggttg cagaaaggtt ccttctaaca acttagagtc
     1261 aaaagtcaaa acggtttctt gagcaagaga tccatcaatt attttatgat atgggatagt
     1321 gatagtattg attcgaggct tttcaaacat tgccccgtta ccagtggtta gccatgttaa
     1381 tgaagcacca gtttccagtg agcattgaat aatccagtca gcaggaaagg tatctctcat
     1441 gtaacgattg gctaatgtac tttttgatac accaatatga tcagctaaag cttgtcttgt
     1501 cttaaatcca taagccaaaa cgattcgctc tattgctgct tttccgcctt ggttaatagt
     1561 aaaaaagttc tcattttgga tgtcttgttc tctattgaga tgttgatgct tgctgtcaca
     1621 attgttgtga cttttttctg cattatttgt aattcttccc ttctctgtat cagtgccaaa
     1681 tgccaaccac tcgaccggca ctccagtctc catgctacag ataacgaccc aatcagcagg
     1741 gaaagtgtca cgggcatacc tgttcgccat tgtactttgc gatataccta ggtgattaca
     1801 gaatgcttgt cgggaagaaa aaccataggc tttcagtatc cgagctataa ccgattgccc
     1861 tccgcgattc tgcatgatta aggatttctt tttttcgtcc acatggcgaa atgtgatttc
     1921 gtgtgttgac ataaccgatt tgtgatccta ttctccgaaa tgtgaagttg aagccacgat
     1981 taaaactgac tcaccacaag ccaataggag atgttgcatc atgaccccta acatttcaat
     2041 aaccctgaat acgccacatg tcacaattga gcgttatagc gaacttactg gtctttcaat
     2101 cgacacaatt aacgatatgc tggctgacgg tcgcatccct cggcatcgcc ttcggaaaga
     2161 caagaaaaga gaaaaggtga tgatcaacct tgctgctctt accgttgatg cacttactga
     2221 ttgcaatgtt gtattcaact agttccattt tgggatgcat caggggtgtc gaccatgttt
     2281 gattaccaag tttccaaaca tccacatttt gatgaagcct gtcgtgcatt cgcattgcgc
     2341 cacaacctgg tgcaactggc agaacgtgct ggcatgaatg tgcagattct gcggaacaag
     2401 ctgaacccag ctcaacctca tttattaacc gcaccagaaa tctggctgct taccgatctg
     2461 actgaagatt caacgctggt agatggtttt ctggcacaga ttcattgtct gccatgtgta
     2521 ccgattaatg aggtggcaaa agagaaactg ccacattacg tcatgagtgc aaccgcagag
     2581 atcgggcgtg ttgcagcagg tgcggtatct ggcgatgtaa aaaccagtgc aggtcgtcgt
     2641 gatgctatca gcagcattaa ctctgtaaca cgactgatgg cgctggccgc tgtttcattg
     2701 caggcccgtt tacaggctaa tcctgcgatg gcgagtgcag ttgataccgt gactggcctc
     2761 ggtgcttcat tcggtttgct gtgaggtgct tatgctgacg aaagaaccat catttgcatc
     2821 gctgctggta aaacaaagtc cggcaatgca ctacgggcac ggctggatca tgggtgagga
     2881 tggtaaacgc tggcatccgt gccgttcaca agatgaattg ctggcagaac tatctacgaa
     2941 aaaacagggg aacaaatggc tattgaaggc gctgcggcga ctgttccatt aagccccggt
     3001 gaacgcctga acggacttaa tcacattgcg gaattaaggg cgaaagtatt tggcctgaat
     3061 attgagtcag agcttgagcg gtttattaaa gatatgcgtg atccacggga tatcaataat
     3121 gaacaaaata aacgggcact ggctgccata ttctttatgg caaaaatccc agctgaacgt
     3181 catagcatca gcattaatga gctgaccact gacgaaaagc gggagctgat taaagcaatg
     3241 aatcattttc gtgcagtggt gagcttattt cccagacggc taaccatgcc gaattaacca
     3301 actaatgaaa ttcatggcgt aaacccgccg ggcatccctt tatctaaatt caggagaatt
     3361 gattatgcgt agtattgaaa ccctcacgac taaaaccgga ccggatgatg cagggcttaa
     3421 tattttactg acagaggctc gtctggaaga ccgccgggca agggctgaag caatggcagc
     3481 tcgccttgat agcctggcgt gtcatatcac atcccgccag ctaaaccacg tcgaagcggc
     3541 agaactgctg cgtgtgactg ctgaagcaat ccagaacgaa gcgcaggaga tccactgatg
     3601 gctgatgcaa tggatctcgt acagcagcgc gttgaagaag aacgccagcg tcatatccgt
     3661 actgcccgcg ccaaaacgcc gggcgtatcc cgcgtgcttt gcattgagtg tgaagcacca
     3721 attccgccag cacgacgtcg cgccattccg ggtgtgcagc tttgcattac ctgtcaggaa
     3781 atcgcagagc tgaaaggcaa acattacaac ggaggtgctg tatgagcacc atcctgaaat
     3841 gggcgggtaa taaaaccgcc attatgccag aactgaaaaa ataccttcct gctggcccgc
     3901 gactggttga acctttcgcg ggttcttgtg ctgtgatgat agagacggat taccccagct
     3961 atcttgttgc ggatattaat cctgatttaa tcaacctcta taaaaaggtt gctgctgatt
     4021 gcgaggcgtt tatatctcgt gccagagctt tatttgagga agcaaacagg gaggtggctt
     4081 attacaacat aaggcaggag tttaattact ccactgaaat tactgatttc atgaaagcgg
     4141 tatatttcct gtatctcaat cgtcatggtt accgtgggtt atgtcgctat aacaagagcg
     4201 ggcatttcaa cattccctat gggaattata aaaatccgta tttccctgaa aaagaaattc
     4261 gcaaatttgc agaaaaagcc cagcgagcaa cgtttatctg cgccagcttt gatgaaacgc
     4321 tggcgatgtt gaaggcgggg gatgtggtgt attgcgatcc gccttattac ggtacgtttt
     4381 ccggctatca cactgatggc ttcactaagg atgaccagta tcacctggca tccgttcttg
     4441 aacatcgatc atctgaaggt catccggtca ttgtttctaa cagtgacaca tccctgattc
     4501 gttcgctgta tcgcaatttc actcaccact acatcaaggc aaaacgcagc atcggcgtgt
     4561 cggctggcga gagtaaatct gcaacagaaa tcattgctgt ttccggggcg cgctgctggg
     4621 tgggatttgc tccttcgcgt ggcgtagata gttctgctgt gtacgaggtg cgtgtatgag
     4681 tcatgacgat atgagcaact ctagcggctt taacgaggcc gctgcatcat tttcatggaa
     4741 cggcccgaaa aaggccatta atccttatct ggacccggcg gaagttgcgc cggagtctgc
     4801 actttcaaac ctgatcactc tgtatgctgc cgataacgag caggaacaac tgcgccgcga
     4861 ggcactgagt gagcaggtct gggagcgtta tttctttaat gaatcccgtg atcctgtcca
     4921 gcgcgaaatg gagcaggata agctcattag ccgggtaaag ctggcgcatg agcagcagcg
     4981 ttttaatcca gacatggtca ttctggctga cgtcaacgcc cagccttccc atatcagcaa
     5041 gccgctgatg caacgtattg aatacttcag cagcctgggc aggccaaagg cttattcccg
     5101 ctatttgcgt gagacgatta agccatgtct ggaacgactg gagcatgtac gcgacagtca
     5161 gctatccact tcttttcgct ttatggcaag ccatgaaggg ctggacggcc tgctgatcct
     5221 gcctgaaatg agtcaggatc aggtgaaacg cctgtctacc cttgtcgctg cgcatatgag
     5281 catgtgtctt gatgccgctt gtggtgattt gtacgccacc gatgatgtta agccagaaga
     5341 aatccgcaag acatgggaaa aggtggcagc ggaaacccta cgtttggatg tcatcccgcc
     5401 tgcgtttgag caactccgtc ggaaaagaaa ccgccgtaaa ccagtgccct atgaactcat
     5461 tccgggctcg ctggcgcgta tgctgtgcgc cgactggtgg tatcggaaat tgtggaagat
     5521 gcgttgtgaa tggcgggaag agcagttgcg tgctgtctgc ctggtcagca aaaaagcatc
     5581 tccctatgtc agctatgaag ctgtgatgca taaacgtgag cagcgccgta agtcgctgga
     5641 gtttttccgt tctcatgaac tggtgaacga agacggcgac acgctggaca tggaagatgt
     5701 ggtaaacgcc agctgcagca acccggcgca tcgccgcaat gagatgatgg cctgtgttaa
     5761 aggtctggag cttatcgcgg aaatgcgcgg tgactgcgcc gttttctaca ccattacctg
     5821 tccgtcacgt ttccattcca cgctcaataa cggcagaccc aatccgacct ggaaaaacgc
     5881 gacggtaaga caaagcagcg attatctggt cgatatgttt gctgcatttc gtaaggcgat
     5941 gcacaaagcc gggttgcgct ggtatggcgt gcgggtggct gagccgcatc acgacggcac
     6001 agttcactgg cacctgttgt gtttcatgcg caaaaaagac cgccgcgcca ttactgcatt
     6061 gttgcgtaag tttgctatcc gtgaagaccg cgaggagctg ggcaataaca cggggccgcg
     6121 ctttaagtct gagctgatta acccgcgcaa aggaacgccg acaagctaca tagcgaaata
     6181 catcagtaag aacattgacg ggcgtggtct ggctggcgag atcagcaagg aaacgggtaa
     6241 atctcttcgt gataacgctg aatacgtgaa tgcctgggcg tctttgcatc gtgttcagca
     6301 attccgcttc tttggtattc cggggcgtca ggcttaccgt gaactgcgat tgctggctgg
     6361 tcaggcggca aggcagcagg gtgacaaaaa agcaggtgtg ccggtactgg ataacccgcg
     6421 ccttgatgcc attctggctg ctgctgatgc tggttgtttt gccacctata tcatgaagca
     6481 gggaggtgta ctggttcccc gtaaatatca cctcatcaga actgcttatg aaattaacga
     6541 agagccgacc gcctatggcg atcacggtat tcgtatttat ggcatctggt cacccattgc
     6601 agagggcaag atctgcactc atgcagtgaa gtggaaaatg gttcgtaagg ccgttgacgt
     6661 tcaggaggcg gcagccgacc agggcgcttg cgccccttgg actcgtggca ataactgtcc
     6721 ccttgctgaa aatttgaacc aacaagggaa agacaaatca gctgatgggg ataccaggac
     6781 ggacattacc cgcatggatg acaaggagtt gcacgattac ctgcacagta tgagcaaaaa
     6841 agagcgccgg gaactggctg caaggttacg cctggtgaaa ccgaaacggc gtaaagacta
     6901 caaacagcga attacagacc atcagcgaca gcagctcgtc tatgaactga agtccagagg
     6961 atttgatggc agcgagaaag aggtcgattt actccttcgc ggcggcagta ttccatcagg
     7021 agcaggcctg cgtatcttct atcggaacca gcgtttgcag gaagatgata agtggcgaaa
     7081 cctgtattaa ttacgcgggt taacaattcg tgctcttaat aataccaggc atatcaggct
     7141 gatgaacgta aaaaaacgtt ttacatcagt aatattatta tatactgtaa atataaacag
     7201 tgtgcgccgg gagaccggtt agagatcaag gggtgaaagt ccccgaccat tgaaggacca
     7261 gcaatccaca aggtccccga gtcatgcgtt gcataccgcg aggtatgggg cgaagcgttg
     7321 acaggggtgt tgacaggcca gccattgagc cacgaaatgt atattaaatt accgggtgcc
     7381 gacgttgtac tgttaacgga aggcaacatc atagggtgcg atactgcgag tgccacatgg
     7441 acccggcggg gtctgagacc ctggcatgtc aatacgatct ctacgcggga accgggagat
     7501 ctcccctctg accatctgcc agtgtcggag atggcccgca ccgggaagac gaggagtcat
     7561 agccggtgat gtacggagag gagaagtcgg actcgctcat agtagcggcg aagcaggcga
     7621 acaacccgaa aggagcggag tcagtggagc gaaggagcgg ggccaagggg aacgcggaac
     7681 agccacacat gcgccggaca cagagccggg aaagcatgtc acagaggctg tcacgcgtgc
     7741 gggaagctgc gaagcagcgg aagaaagaac ggtttacagc attgttccac ctgctgacag
     7801 tcgaagcact ggaagccgca ttcctctccc tgagcaggaa agcggccgcc ggagtggatg
     7861 gcatcaggtg gatggactac gccggaaaca tgaagaacaa cataacagat ctgcaccgga
     7921 ggctacatca gggcagctac agggcgcagc ccggcaggcg tcactacatc ccaaaagcgg
     7981 atggaaaaca acgcccgctc ggcatcgcct cgctggagga caagatcgtc cagtatgcgc
     8041 tggtgaaaat cctgaacgca gtctatgaaa acgactttat ggggttctca tacgggttca
     8101 gacccgggcg aagccagcac gatgcactgg acgcactggc cacagggctg gtacgcacta
     8161 acgtaaactg ggtactggat gccgacatca gtcagttctt cgacagggtg agccacgaat
     8221 ggctgatcag gttcacagag catcggatcg gcgaccggag ggtaatcagg ctcatacgta
     8281 agtggctcac agccgggacg tcggaggagg gtcaatggcg agcaacggag gaaggcaccc
     8341 cacagggtgc ggtcatctca ccgctgctgg caaacatata cctccactac gtcttcgatc
     8401 tgtgggcgca tcagtggcga cgtcgctatg ccacaggcaa tgtggtaatg gtcagatacg
     8461 ccgatgacat cgtcatcggg ttcgacaaac gatacgatgc ccggcgcttc cgtatagcca
     8521 tgcagcgcag actgagggag ttcggactca cggttcaccc ggagaaaacc cgtctgatgg
     8581 agttcggccg cttcgctgcc gaaaaccgtg ccatcagggg aaaaggcaaa ccagaaacgt
     8641 tcaacttcct cgggttcacg cacatcagcg ggaaagatcg caacggcagg ttcatgctga
     8701 tacgaaagac ccgccgggat cggatgacgg caactctgaa agccatcaaa gacggtctgc
     8761 gaaggcgctg gcattactca atccccgaac agggaaaatg gctcaggaga gtggttcagg
     8821 gatacctgaa ctatcactcg gtaccgggca acttccccac catgcagaag ttcaggacac
     8881 acgtaacaaa cctctggcgc cgggcgctca ggcgcaggag ccagaaggat gatacgacct
     8941 ggacgaaagc aaacaaactg gcagccgcat ggctaccaag ggttcgggtt cttcatccat
     9001 ggcctgtgga gcggttcacc gccagacacc cgaggcagga gcccggtgcg taaatcgcgc
     9061 acgccgggat ctgtgcgggg ggtatccggt aacgggtatc cctaccgcga c
//
