LOCUS       NZ_CP072973             2495 bp    DNA     circular CON 14-AUG-2023
DEFINITION  Enterobacter sp. BWH 37 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP072973
VERSION     NZ_CP072973.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02138600
            Assembly: GCF_018075245.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter sp. BWH 37
  ORGANISM  Enterobacter sp. BWH 37
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Multi-genus dynamics of carbapenem resistance within hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2020) Infectious Disease and Microbiome Program,
            Broad Institute, 415 Main Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: BWH37
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 168x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/14/2023 03:35:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,191
            CDSs (total)                      :: 5,075
            Genes (coding)                    :: 4,968
            CDSs (with protein)               :: 4,968
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 38 of 107
            Pseudo Genes (incomplete)         :: 69 of 107
            Pseudo Genes (internal stop)      :: 17 of 107
            Pseudo Genes (multiple problems)  :: 15 of 107
            Pseudo Genes (short protein)      :: 1 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072973.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter sp. BWH 37"
                     /mol_type="genomic DNA"
                     /strain="BWH 37"
                     /isolation_source="Rectal Swab"
                     /db_xref="taxon:1329836"
                     /plasmid="unnamed3"
     gene            complement(join(2301..2495,1..24))
                     /locus_tag="IBG18_RS25815"
     CDS             complement(join(2301..2495,1..24))
                     /locus_tag="IBG18_RS25815"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            1232..1459
                     /locus_tag="IBG18_RS25805"
     CDS             1232..1459
                     /locus_tag="IBG18_RS25805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1825..2010
                     /locus_tag="IBG18_RS25810"
     CDS             1825..2010
                     /locus_tag="IBG18_RS25810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2058..2145)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          289..817
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 ggctccggaa gaaaaaccaa acatggctta cctactgtta acggcaacgc agaagaaagg
       61 tttacagggt agcgggcagg gatgaaaaaa aaaaaattgc agtcaggccg gaacggctgc
      121 tgaaccggta caggcgggtt ccgggcgcag cgccggacca gtcacggagc actagcggag
      181 tgtatactgg cttaagatgt agcatggcag taacacatag cattaatgcc acataagaca
      241 ggcgcagaaa gtgaaagaaa acgcgtcagg agaaaatgtg atacagaata tattccgctt
      301 cctcgctcac tgactcgctc cgctcggtcg ttcggctgcg gcgagcggga cgggcttacg
      361 gatggatcag aaatttcctg ggggatgcca ggaagatact taacgaagaa agcataaggc
      421 cgcgccaaag ccgtttttcc ataggctccg cccccctgac aagcatcaca gaatctgacg
      481 ctcaaatcag tggtggcgaa acccgatgcg gcttaaagat accaggcgtt tccccctggt
      541 ggctccctcg tgctcccccg cattcctgcc tttcggttta ccggtgtcac tccgctgtta
      601 tggccgcggt ttctcattcc acgcctgaca ctcagttccg ggaaggcagt tcgctccagg
      661 atggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat
      721 caacttgagt ccaacccgga aagacacgac taatcgccat tggcaacggc cattggcaaa
      781 caggatgtgc gagagatgtc tcaagagttc ttgaagtggc ggccttagtg aggctacact
      841 gaaaggacaa atttggtgac atgtctcgca caagactgtt accacggtta ggggatctgc
      901 caggattacc taaccttcga aaaaccacct gccagggtgg ttttttcgtt tacagggcaa
      961 gagattacgc gcggaaaaaa aggatctcaa gaagatcctt tataaattag acatccaacc
     1021 tcacaaagag gaatacttaa atcatcttgg tatatgtatc ggccggatta catctcatat
     1081 aagcctcctc cccgcctgac accacacttt gaccatagca cctcgcgcct aactcgatat
     1141 acagcccacc atcaagcctc ttttcacata aaaaatgacc tatagttaga tggttagtga
     1201 ccaaaactga tatggataaa aggagctggc tttgaaaaag atttttgttt cagtgttcgc
     1261 agcggcagtt gcgctttcag ccctcaccgg ctgcacgcgc acaagctatg ccatacacac
     1321 caatgacggt cgcactattg tcagcgacgg caaacctaaa gaatcagact caggcctgct
     1381 tggctataca gacgcgaatg gtgtcaaaca gcagatcaat aaaaccgacg ttaaagaagt
     1441 ctccgaaatt ccgcactgag catcttctcc gaaaaaggcc gctaatgtgg cctttttctt
     1501 tacctgtggt tctccgccaa aatcccagca aattgcatca ccaaagctaa aagctttcag
     1561 ggttgtctaa aaagcacaag acataagagg aagtgcggta ttttataatc aagcccccaa
     1621 gaatttacca taacatccgt tgcccgcacc gcctgagacg cgttcagcgc gttcctgacg
     1681 aaaccatgac aaaaaccaca acaaaccacc ccggaacccg tcagaaacgc gcctgttaaa
     1741 ttttaacggc atgcatgact atgcaccaga atgacgccat gctcttttca cgcaaaaatc
     1801 atcaccagac ggggaaaatc accagtgacc agacaggaat ccgccgccct caatatggcc
     1861 aaatttatcc gcgcacagac acttctcctc cttgagcggc tcgagcagat ggatctggat
     1921 gaggctgccg gctgctgtga gcacctgcac gatcaggccg aagcgcttta cgccatgctg
     1981 aacgcacaga taggcgagga aaatgcgtga aaatcggtga acgggtgcgc aattcagtgc
     2041 gcggccgtga ggcgatggcg gggtgtcggg gcgcagccct gaccagggta tttgtgatgc
     2101 cggcgcgtgc gcggtattac aaatgcacat cctgtcccgg aacggacacc gggaaacagc
     2161 aaaaaaaacc gggctgcacg cccggaactc aatcaagtta gattagatta ctctcactcg
     2221 tccataacag catcatggaa cgacgaccac cgtccgtgac ggccgcctcg tttaagtatg
     2281 gacagaaata cagaaaatgc tcaggacgaa atgtaatgaa tgcgaacgga ttcaagaaat
     2341 tcgagcatga cagtccttac ggccggttcg gtttcagaca aaatctgccg gtatgcatcc
     2401 agcatcatgg ctccggcatc ccctccggca cgccgtagcc agaccgaaac aacggacaca
     2461 agcaggtgtc gctcatcatc actaagagtc atcag
//
