LOCUS       NZ_CP072969             2264 bp    DNA     circular CON 14-AUG-2023
DEFINITION  Enterobacter sp. MGH 14 plasmid unnamed8, complete sequence.
ACCESSION   NZ_CP072969
VERSION     NZ_CP072969.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02138554
            Assembly: GCF_018074945.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter sp. MGH 14
  ORGANISM  Enterobacter sp. MGH 14
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Multi-genus dynamics of carbapenem resistance within hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2020) Infectious Disease and Microbiome Program,
            Broad Institute, 415 Main Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.3
            Assembly Name                     :: MGH14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 232x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/14/2023 03:33:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,348
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,100
            CDSs (with protein)               :: 5,100
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 43 of 133
            Pseudo Genes (incomplete)         :: 95 of 133
            Pseudo Genes (internal stop)      :: 25 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            Pseudo Genes (short protein)      :: 1 of 133
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072969.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Enterobacter sp. MGH 14"
                     /mol_type="genomic DNA"
                     /strain="MGH 14"
                     /isolation_source="respiratory"
                     /db_xref="taxon:1329823"
                     /plasmid="unnamed8"
     gene            1147..1464
                     /locus_tag="IBG17_RS26590"
     CDS             1147..1464
                     /locus_tag="IBG17_RS26590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000718044.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAANVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEATGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAV
                     Q"
                     /name="hypothetical protein"
     gene            1716..1904
                     /locus_tag="IBG17_RS26595"
     CDS             1716..1904
                     /locus_tag="IBG17_RS26595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1952..2218)
                     /locus_tag="IBG17_RS26600"
     CDS             complement(1952..2218)
                     /locus_tag="IBG17_RS26600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223173001.1"
                     /translation="MKRSQLTLIDGCFMFGFSPGELMNLCPDERLLFVRMISAMLRRSG
                     GDAGAVMFEAYRHIVSDTNQARRSYMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     Typing          complement(99..271)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.954"
                     /coverage="100.0"
                     /evalue="2.04e-62"
     Typing          196..741
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.989"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 aacatcacag gtaaacagaa acgtcgggtc gatcgggaaa ttctttcccg gacggcgcgg
       61 ggttgggcaa gccgcaggcg cgtcagtgct tttagcgggt gtcggggcgc agccctgaac
      121 cagtcacgta gcgctagcgg agtgtatact ggcttactat gtaggcacgg gtgcgggaat
      181 cagtgaagtg cttcatgtgg caggaggaaa atgcagcagc agagcgtaag cggaatatat
      241 gagacaggag atattccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      301 ggcgagcggt gcgggcttac gaatggggcg gaaatttcct gggagatgcc aggaagaaac
      361 ttaacaggga aacgataagg ccgcggcgaa gccgtttttc cataggctcc gcccccctga
      421 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag
      481 ataccaggcg tttccccctg gtggctccct cgtgcgctct cctgttcctg cctttcggtg
      541 taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga cactcggttc
      601 cgggaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgactg
      661 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacacg acaaagcgcc
      721 actggcagca gccactggta acaggattag cagagcgagt taatgtagat ggtgctatag
      781 agttcttgaa gtggtggcct aactacggct acactggaag gacagtattt ggtatctgcg
      841 ctctgctgaa gccagttacc aggttaagca gttccccaac tgacttaacc ttcgaaaaac
      901 cacctcccca ggtggttttt tcgttttcag agcaagagat tacgcgtaga aaaaaaggaa
      961 ctcaagaaga tcctttctat gctcaaccac cgcatggccc atagccgcgc tggccgttgc
     1021 cggtcagctc cagaatcctc gaccaatatg gaaccttgcc cctcccaaaa ttcaactaaa
     1081 aaaccctcat ttcctgccga taaaatgggt atctcttaac tatgcctaaa taaaataagg
     1141 ataattttga aaaaaatatt catcctatcg gcagcgctga tcatggcagg atgcgcaaca
     1201 aagcaatatc cacaggccgc gaacgtcacc ggtccagaat cttccgcaat ggattgcaaa
     1261 gcggtagctc tggaaatcgc aaaaacgcgc agtgtacaac aagaaattga ggcaaccggc
     1321 gaatttgacg ggcgcaccgt attaggtttc ctgggtgatt ttgggatagg aaatggcatg
     1381 gcaaaaggcg aagcccgcaa aaaagcagct aaccggctaa atcagctgga agccctgaag
     1441 gctgcaaagt gtgcagttca ataaggtaca aaaaagccct gcggggcttt ttttgtacct
     1501 gccaatcagc tggatttacc ataacatccg ttgcccgcac cgcctcaggc gccctcaggg
     1561 cgtttctgag gactccggcc tgaaacatac acctttctgt gcccatatcg agaaaaaacg
     1621 cccctgatga tttctgcgta cctgcatggc tatgcactca agtgaatgaa gtaagatttc
     1681 agtctgcgat caccataaaa ccgggaaaaa gccgtatgac aaaacaggaa tcagccgcac
     1741 tgaacatggc aaaatttatc cgggcgcagt cgctgctgct gctcgaaaaa ctcgacgtgc
     1801 tcgatcttga tgacgaggca gccgactgtg agcgaatgca cgaacaggca gaagcgcttt
     1861 atcagaagct gagcgcacgc ttcggtatac aggacggcga ataaagagac gaaaagaacg
     1921 ctcacttaga cgaaaagcgt atccagggca actatgtata tccaccgtgt acatagtcat
     1981 gcctgacgct ttcgagcagg tcgagcatgt aggaacgacg agcttgattg gtatcggaca
     2041 caatatggcg atacgcctca aacataacag cacccgcatc accgcccgaa cggcgaagca
     2101 tagcggaaat catccgcaca aagagcagac gttcatcggg gcaaaggttc ataagttctc
     2161 ctggtgaaaa accaaacatg aagcacccat ctattaacgt cagctgtgaa cgcttcatta
     2221 cgcttcatta aaataggtaa acccgggaaa aagatccaaa cgtt
//
