LOCUS       NZ_CP072968             2495 bp    DNA     circular CON 14-AUG-2023
DEFINITION  Enterobacter sp. MGH 14 plasmid unnamed7, complete sequence.
ACCESSION   NZ_CP072968
VERSION     NZ_CP072968.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02138554
            Assembly: GCF_018074945.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter sp. MGH 14
  ORGANISM  Enterobacter sp. MGH 14
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Multi-genus dynamics of carbapenem resistance within hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2020) Infectious Disease and Microbiome Program,
            Broad Institute, 415 Main Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.3
            Assembly Name                     :: MGH14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 232x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/14/2023 03:33:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,348
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,100
            CDSs (with protein)               :: 5,100
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 43 of 133
            Pseudo Genes (incomplete)         :: 95 of 133
            Pseudo Genes (internal stop)      :: 25 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            Pseudo Genes (short protein)      :: 1 of 133
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072968.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter sp. MGH 14"
                     /mol_type="genomic DNA"
                     /strain="MGH 14"
                     /isolation_source="respiratory"
                     /db_xref="taxon:1329823"
                     /plasmid="unnamed7"
     gene            complement(274..501)
                     /locus_tag="IBG17_RS26575"
     CDS             complement(274..501)
                     /locus_tag="IBG17_RS26575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1736..1927
                     /locus_tag="IBG17_RS26580"
     CDS             1736..1927
                     /locus_tag="IBG17_RS26580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021243041.1"
                     /translation="MTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQILSETEPAV
                     RTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(2218..2403)
                     /locus_tag="IBG17_RS26585"
     CDS             complement(2218..2403)
                     /locus_tag="IBG17_RS26585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2084..2171
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(917..1445)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.411"
                     /coverage="88.0"
                     /evalue="3.17e-180"
ORIGIN
        1 gcgcgtttct gacgggttcc ggggtggttt gttgtggttt ttgtcatggt ttcgtcagga
       61 acgcgctgaa cgcgtctcag gcggtgcggg caacggatgt tatggtaaat tcttgggggc
      121 ttgattataa aataccgcac ttcctcttat gtcttgtgct ttttagacaa ccctgaaagc
      181 ttttagcttt ggtgatgcaa tttgctggga ttttggcgga gaaccacagg taaagaaaaa
      241 ggccacatta gcggcctttt tcggagaaga tgctcagtgc ggaatttcgg agacttcttt
      301 aacgtcggtt ttattgatct gctgtttgac accattcgcg tctgtatagc caagcaggcc
      361 tgagtctgat tctttaggtt tgccgtcgct gacaatagtg cgaccgtcat tggtgtgtat
      421 ggcatagctt gtgcgcgtgc agccggtgag ggctgaaagc gcaactgccg ctgcgaacac
      481 tgaaacaaaa atctttttca aagccagctc cttttatcca tatcagtttt ggtcactaac
      541 catctaacta taggtcattt tttatgtgaa aagaggcttg atggtgggct gtatatcgag
      601 ttaggcgcga ggtgctatgg tcaaagtgtg gtgtcaggcg gggaggaggc ttatatgaga
      661 tgtaatccgg ccgatacata taccaagatg atttaagtat tcctctttgt gaggttggat
      721 gtctaattta taaaggatct tcttgagatc ctttttttcc gcgcgtaatc tcttgccctg
      781 taaacgaaaa aaccaccctg gcaggtggtt tttcgaaggt taggtaatcc tggcagatcc
      841 cctaaccgtg gtaacagtct tgtgcgagac atgtcaccaa atttgtcctt tcagtgtagc
      901 ctcactaagg ccgccacttc aagaactctt gagacatctc tcgcacatcc tgtttgccaa
      961 tggccgttgc caatggcgat tagtcgtgtc tttccgggtt ggactcaagt tgatagttac
     1021 cggataaggc gcagcggtcg gactgaacgg ggggttcgtg catacagtcc atcctggagc
     1081 gaactgcctt cccggaactg agtgtcaggc gtggaatgag aaaccgcggc cataacagcg
     1141 gagtgacacc ggtaaaccga aaggcaggaa tgcgggggag cacgagggag ccaccagggg
     1201 gaaacgcctg gtatctttaa gccgcatcgg gtttcgccac cactgatttg agcgtcagat
     1261 tctgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggctttggcg cggccttatg
     1321 ctttcttcgt taagtatctt cctggcgtcc cccaggaaat ttctgatcca tccgtaagcc
     1381 cgtcccgctc gccgcagccg aacgaccgag cggagcgagt cagtgagcga ggaagcggaa
     1441 tatattctgt atcacatttt ctcctgacgc gttttctttc actttctgcg cctgtcttat
     1501 gtggcattaa tgctatgtgt tactgccatg ctacatctta agccagtata cactccgcta
     1561 gtgctccgtg actggtccgg cgctgcgccc ggaacccgcc tgtaccggtt cagcagccgt
     1621 tccggcctga ctgcaatttt ttttttttca tccctgcccg ctaccctgta aacctttctt
     1681 ctgcgttgcc gttaacagta ggtaagccat gtttggtttt tcttccggag ccctgatgac
     1741 tcttagtgat gatgagcgac acctgcttgt gtccgttgtt tcggtctggc tacggcgtgc
     1801 cggaggggat gccggagcca tgatgctgga tgcataccgg cagattttgt ctgaaaccga
     1861 accggccgta aggactgtca tgctcgaatt tcttgaatcc gttcgcattc attacatttc
     1921 gtcctgagca ttttctgtat ttctgtccat acttaaacga ggcggccgtc acggacggtg
     1981 gtcgtcgttc catgatgctg ttatggacga gtgagagtaa tctaatctaa cttgattgag
     2041 ttccgggcgt gccgcccggt tttttttgct gtttcccggt gtccgttccg ggacaggatg
     2101 tgcatttgta ataccgcgca cgcgccggca tcacaaatac cctggtcagg gctgcgcccc
     2161 gacaccccgc catcgcctca cggccgcgca ctgaattgcg cacccgttca ccgattttca
     2221 cgcattttcc tcgcctatct gtgcgttcag catggcgtaa agcgcttcgg cctgatcgtg
     2281 caggtgctca cagcagccgg cagcctcatc cagatccatc tgctcgagcc gctcaaggag
     2341 gagaagtgtc tgtgcgcgga taaatttggc catattgagg gcggcggatt cctgtctggt
     2401 cactggtgat tttccccgtc tggtgatgat ttttgcgtga aaagagcatg gcgtcattct
     2461 ggtgcatagt catgcatgcc gttaaaattt aacag
//
