LOCUS       NZ_CP072967             2811 bp    DNA     circular CON 14-AUG-2023
DEFINITION  Enterobacter sp. MGH 14 plasmid unnamed6, complete sequence.
ACCESSION   NZ_CP072967
VERSION     NZ_CP072967.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02138554
            Assembly: GCF_018074945.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter sp. MGH 14
  ORGANISM  Enterobacter sp. MGH 14
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Multi-genus dynamics of carbapenem resistance within hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2020) Infectious Disease and Microbiome Program,
            Broad Institute, 415 Main Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.3
            Assembly Name                     :: MGH14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 232x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/14/2023 03:33:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,348
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,100
            CDSs (with protein)               :: 5,100
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 43 of 133
            Pseudo Genes (incomplete)         :: 95 of 133
            Pseudo Genes (internal stop)      :: 25 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            Pseudo Genes (short protein)      :: 1 of 133
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072967.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Enterobacter sp. MGH 14"
                     /mol_type="genomic DNA"
                     /strain="MGH 14"
                     /isolation_source="respiratory"
                     /db_xref="taxon:1329823"
                     /plasmid="unnamed6"
     gene            584..1183
                     /locus_tag="IBG17_RS26565"
     CDS             584..1183
                     /locus_tag="IBG17_RS26565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="WP_001029633.1"
                     /translation="MAKAKVLINDTDEAVIKNALDVEDMNEVNINVTRKKKTGFLGTYS
                     IVFHDSVKTLSKMNLKPNAYKIVMYLFGIIEMGNILVNFSQKKIADDLGLQPSNVSRAF
                     KELFDKGVLIKDSEDGHVYLNSNLCTMGVPKNFDQKRMKSLQKSNIETEDFKNQINLFT
                     PTNTAKIKTNDKKVIKKKEKENVLEFPMQNDWVDIE"
                     /name="Crp/Fnr family transcriptional regulator"
     gene            1427..2710
                     /locus_tag="IBG17_RS26935"
     CDS             1427..2710
                     /locus_tag="IBG17_RS26935"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_227524674.1"
                     /translation="MKSQFMHVELYSREEPAKKTINKSKNINHKSDVRTTTVNGVLSEM
                     KREEGFTSHLETIAAPEVLYGSIDALERSIERYEAEFKTTDKNGKEKKLRKDACILLAG
                     VVSLDRADEEIWDEYKTKAIEYLKNKYGENLKCVVEHTDETNPHFHFYVVGNKDQDLNL
                     LHDGKLAVSKLAKEDKKKLHQTAYTEAMIKFQDDFYVQVSNKFGLSRTGEEPRERYKSR
                     PDYLRFKKRQETALNILANLEEFEEKELKKAKQAGFNVGIKNFDETNFFRKLDWRVKRI
                     KKEKDNEIKKLSDENDNLNIDKSNLQHELDMSKIELSDAYSQRNEASRKNSVYIDKVNA
                     LKKENKLLLPYKDQFDLKLKQEKEPLQAEVKELTSENTNLKNENTKLKKENLDFKEFIL
                     GVKNYYGSKFQEWYEEVFNPKTKTKPKF"
                     /name="plasmid recombination protein"
ORIGIN
        1 tggttttgca cgcagttagt tacctttaaa tatcagtcac ttaaatacaa aaaatgcagg
       61 ttgggaaaat gtggtcaggg agctggccgc attggggtgg gggtaccttc ataacgcctt
      121 ggtgcgttgt gaagaggggg aacggggcgg ggttccccct ttggtatgta actacatata
      181 aaacaaagaa atactgttaa actttgaacc gtgagaactg cagaaccact tgtggttctg
      241 tggttcgagg ggttggcctg cccggcgagg gcggttttgc agttgatttt acggtttctt
      301 ctttcttatt ttcttacttc ttaagcaccc tttaaagcct tatatatcaa cactttacca
      361 aaaaaaaaat tatgaaaatc ataattttcg attatgaaaa tcataatgca aaattatgaa
      421 aatcataatc agacttatga aaatcataat tttgcataca ggtgcattga tatgatttga
      481 tgttgaaacg ataactttct tatcgttttg atattgacat tgcatttaga tcggttataa
      541 atgcaacata ggttattatt tttattagaa aaaggaatat aaaatggcaa aagcaaaagt
      601 tttaataaac gatacagatg aagcagtaat aaaaaacgca ttagatgttg aagatatgaa
      661 cgaagtaaat attaacgtca cgagaaaaaa gaaaaccgga tttttaggga cttattcaat
      721 agtatttcat gactctgtga aaacactatc taaaatgaac ttaaagccaa atgcttataa
      781 gattgtaatg tacttattcg gcattataga gatgggtaat attttagtta acttttcaca
      841 aaagaagata gctgatgatt taggcttgca gccgtctaat gtatcaagag ctttcaaaga
      901 gctttttgat aaaggggttt taatcaaaga tagcgaagac ggacacgttt acttaaattc
      961 aaatctatgc actatgggtg ttccgaaaaa ttttgatcaa aagagaatga aaagcttaca
     1021 gaaatcaaat attgaaactg aagattttaa aaatcagatt aatttattta ctccaacaaa
     1081 caccgcaaaa ataaaaacta atgataaaaa agttatcaag aaaaaagaaa aagaaaacgt
     1141 tttagagttt ccaatgcaaa atgattgggt tgatattgaa tagtaaaact aacactaccg
     1201 cacttcgttt gctgaatccc taaacctcgc aaatataaaa tttgcaagct ttagggattt
     1261 gcttttttgt tttgaagttg actttaaccc cgtagggccg catctatgga gcagcatatg
     1321 aaatatgcta gcgtaataga tatagtcggt tcctatagct attgacagaa aaacaataaa
     1381 tgttaattac atatataagg aaaattaaaa tataggagaa tttaaaatga aatcacaatt
     1441 tatgcatgta gaactctaca gcagagaaga accagctaaa aagacaataa ataaaagtaa
     1501 aaatataaat cataaatctg atgttagaac aacaaccgta aacggtgtgt tgtcagaaat
     1561 gaaaagggag gagggtttta catcccattt agaaaccata gctgcaccgg aggtgctcta
     1621 tgggtctata gatgccttag aacgctctat agagcgttac gaggccgaat ttaagactac
     1681 agacaagaac gggaaggaga agaagttacg caaagatgca tgcatcttgc tagctggtgt
     1741 ggtttctcta gatcgagctg atgaagaaat ttgggatgaa tataagacca aagcaattga
     1801 atatttaaaa aataaatatg gtgaaaattt aaaatgtgtt gttgagcata cagacgaaac
     1861 caacccacat tttcattttt acgtcgttgg aaacaaggat caagatttaa atttgcttca
     1921 tgatggaaag ctcgcagtat ctaaattagc aaaagaagat aagaaaaaat tacatcaaac
     1981 agcttacaca gaagctatga tcaaatttca agatgatttt tatgtacagg tatcaaataa
     2041 atttggattg agcagaacgg gcgaggagcc gcgagaacgc tataaatcac gccctgatta
     2101 cttgagattt aaaaaaagac aagaaacagc attaaatata ttggccaatt tagaagagtt
     2161 tgaagaaaaa gaattaaaaa aagcaaaaca agctggcttt aatgttggta ttaaaaattt
     2221 cgatgaaact aattttttta ggaaactcga ttggagagta aaaagaataa aaaaagaaaa
     2281 agacaatgag attaaaaaat tatcggatga aaacgataat ttaaatatag ataagtcaaa
     2341 tttacagcat gaattagata tgagtaaaat tgagctttca gatgcatata gtcaaaggaa
     2401 tgaagcatcc agaaaaaata gcgtttatat tgataaagta aacgctctaa aaaaagaaaa
     2461 taaattatta cttccataca aagatcaatt tgatttgaaa ttaaagcagg aaaaagagcc
     2521 attgcaagca gaagtcaaag aattgacttc tgaaaatacc aatttgaaaa atgagaacac
     2581 aaaattaaaa aaagaaaatt tagatttcaa agaatttatt ttgggtgtta aaaattacta
     2641 tggatctaaa tttcaggaat ggtacgagga agtattcaac ccaaaaacaa aaacaaaacc
     2701 caaattttaa ttgaatattt tcatatttgc cgcctatgtg gtgtatgaga caccaccaga
     2761 agcggcctga cagcgtttag ctgtcatagc tgggatttat cccaaaaaaa g
//
