LOCUS       NZ_CP072966             3223 bp    DNA     circular CON 14-AUG-2023
DEFINITION  Enterobacter sp. MGH 14 plasmid unnamed5, complete sequence.
ACCESSION   NZ_CP072966
VERSION     NZ_CP072966.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02138554
            Assembly: GCF_018074945.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter sp. MGH 14
  ORGANISM  Enterobacter sp. MGH 14
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Multi-genus dynamics of carbapenem resistance within hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2020) Infectious Disease and Microbiome Program,
            Broad Institute, 415 Main Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.3
            Assembly Name                     :: MGH14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 232x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/14/2023 03:33:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,348
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,100
            CDSs (with protein)               :: 5,100
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 43 of 133
            Pseudo Genes (incomplete)         :: 95 of 133
            Pseudo Genes (internal stop)      :: 25 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            Pseudo Genes (short protein)      :: 1 of 133
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072966.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Enterobacter sp. MGH 14"
                     /mol_type="genomic DNA"
                     /strain="MGH 14"
                     /isolation_source="respiratory"
                     /db_xref="taxon:1329823"
                     /plasmid="unnamed5"
     gene            307..522
                     /locus_tag="IBG17_RS26540"
     CDS             307..522
                     /locus_tag="IBG17_RS26540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            690..1022
                     /locus_tag="IBG17_RS26545"
     CDS             690..1022
                     /locus_tag="IBG17_RS26545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            complement(1231..1401)
                     /locus_tag="IBG17_RS26550"
     CDS             complement(1231..1401)
                     /locus_tag="IBG17_RS26550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004193911.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNWVHGAEIV
                     ENAYRVLEVKA"
                     /name="hypothetical protein"
     gene            2040..2363
                     /locus_tag="IBG17_RS26555"
     CDS             2040..2363
                     /locus_tag="IBG17_RS26555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2572..>3006
                     /locus_tag="IBG17_RS26930"
                     /pseudo=""
     CDS             2572..>3006
                     /locus_tag="IBG17_RS26930"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     ARG             691..1022
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          1753..1944
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.795"
                     /coverage="89.0"
                     /evalue="1.57e-69"
     Typing          complement(1290..1770)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.867"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          4..620
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="86.058"
                     /coverage="99.0"
                     /evalue="6.0100000000000005e-105"
     Typing          2354..3222
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.552"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gccatgagcc agcgcacgaa agggatgctg cgcatggtca gccagacgtg gctgaccatc
       61 gttctggtct ccgcgctgct gatagcgtcg agcgcgggca ttctgtggtg gcaggggcag
      121 cagatactcg agaactatac gaccatccgg gagcagaaga gcacgcaggc catgctgtca
      181 gagaggaaca gcggcgtaca gctctcgacc tgcggcgagc agagacgccg ctgcgtgagg
      241 gtgaacccgg aagcgggaca gttcggagag gactcgagct ggatgatact ggcggggaaa
      301 tagcacatga cggagctgga aaaacagttg ctgagcgcat tagagcagct acagcaggac
      361 tactcgaaaa ggctggacga gtgggagaac gccttcgcgg aatggcggac gatgtctggt
      421 cttattcaac gggagaacgc ggcgctgaac gagcgcgtca cggtcttgag caggcaggtg
      481 cagagtttga gcgagcagct gcgccggttg tcgaaaggct gaacgcgata gaaactcacc
      541 ggcagcagga gcgggcggtg cagcaccaga aagcgctgga gctggaacga tcgcagtggc
      601 agcatcatgg tccttcgcta tgataagccg ggttctatta attagttgtt atgtaaagat
      661 atttaaattt ttcaacatga ggtattttta tgaatacata tattttcctg ggaggggcta
      721 ttgtggctga agtcataggc accaccttta tgaaatattc agaaggattt acacgcctgt
      781 ggccgtctgt aggaactgca ataagttatt gcgctgcctt ttatctgctt tcccaaacat
      841 tggaaaatat acctaccggt attgcttacg caatttggtc tggtgcggga attgtactaa
      901 taagcctagc tggttggttg gttagcggtc aaaaacttga ttggcccgca attgcaggaa
      961 tgttccttat ttgcgttgga gttttggtta ttaatatttt ttcaaaatct tctgcgcatt
     1021 aactttttac cgaagcatat tggccaaagc aaaaggattt ccttgagatc ctttctttct
     1081 gcgcgtaatc tttttccctg taaacaaaaa aaccaccgct accaacggtg gttcgtttgc
     1141 cggaattcag agctaccaac tctttgttcc gatggtaact ggctttcacg gagcgcagat
     1201 acctaaactg tcctttcagt gtagccgcac tcaggctttc acttcaagaa ctctgtaggc
     1261 attctctaca atctctgctc cgtgaaccca gttaccaatg gctaccgcca ctggcgattt
     1321 gtcgtgtctt tccgggttgg actcaagttg atagttaccg gaacgggcgt ggtagtcgga
     1381 ctgaacgggg ggttcgtgca tacagtccat cctggagcga actgcctacc cggaaccgag
     1441 tttcaggcgt ggaatgagaa accgcggcca taacagcgga atgacaccgg taaaccgaaa
     1501 ggcaggaatg cgggggagca cgagggagcc accaggggga aacgcctggt atctttaagc
     1561 cgcatcgggt ttcgccaccg ctgatttgag cgtcattttc gtgatactcg tcaggggggc
     1621 ggagcctatg gaaaaacggc aactccgcat ccttttcttg gcctttcctg cttcgagcgg
     1681 catttgctta tcccgtaatc tgtgagctgg taacgcccgc cgcagtctca cggcaggagc
     1741 gtagcgaccg agtgagcgag gaggcgtcat ttcatcacca cctgcatttt gcacttacgc
     1801 ccggcagccg catttttccc ctgtcacatg aagcactttc ataaattgcc actccgtcat
     1861 aacatgttta gccagtatac actccgctag cgctacgtga ctggttcagg actgcgcccc
     1921 gaaacccgct aaaaccactg ccgcgcctgc ggcttgtcca acaccgtgcc gaccgggaaa
     1981 agatttcccg tccgtcccgg cgttatcagg aggccggatt ggcagacaaa cgcagcaaaa
     2041 tgctcaccat gtgggtgact gaggacgagc accggcgtct gctggagcgc tgcgagggta
     2101 agcaactcgc ggcctggatg cggcagacgt gcctggacga gaagcccgca cgcgccggca
     2161 aacttccgtc gatctcgccg gcgctgcttc gtcagcttgc cggcatgggg aacaacctta
     2221 accagattgc ccgccaggtt aacgccggtg gtggaagcgg acacgaccgc gtgcagattg
     2281 tcgccgcgct gatggccatc gatgccggac tcgagcggtt gcggcatgcc gtactggaaa
     2341 agggtgctga tgatgatcgt taagtttcat cctcgggggc gcggcggcgg cggcgggccg
     2401 gtcgattatc tgctgggtaa agatcgccag cgcgacggcg ccagcgttct gcaggggaag
     2461 ccggatgaag tccgggagct tatcgacgcc tcgccctacg ccaaaaagta cacttccggc
     2521 gttctgtctt ttgccgaaca ggatttaccg cccggccagc gcgaaaagct gatggcgagt
     2581 ttcgagcggg ttctgatgcc cggactcgat aaagaccagt acagcgtgct gtgggttgag
     2641 caccgggaca aggggcggct ggagctgaac ttcctgatcc cgaacacgga gcttctgacc
     2701 ggcaagcggc tacagccgta ttacgaccgc gccgaccgtc cgcgcatcga tgcctggcag
     2761 accatagtga acggcaggct ggggctgcat gacccgaacg cgccggagaa ccggcgggtg
     2821 ctggtctcgc cttccgcgct gccggaagcg aagcaggaag ccgcccaggc gattacgagc
     2881 ggcctgcttg cccttgcctc atccggggag ctgaaaacgc gtcaggacgt cactgaggcg
     2941 ctggaaagcg caggttttga ggtggtgcgc accacaaaaa gcagcatcag cattgccgac
     3001 ccggacgggg ggcgaaacat ccgactgaag ggagccatct atgaacagtc ttttaacgct
     3061 ggcgaaggac ttagagcaga aatcgaaagc gcagcagcag actaccggcg agatgctgaa
     3121 agccgcattc agcgagcacg agaagtctgt cagagcggaa ctgagcgaaa gcgagaagag
     3181 aatcagcgcc gccatcctcg accacgaccg gaagctgtcc tca
//
