LOCUS       NZ_CP072965             4209 bp    DNA     circular CON 14-AUG-2023
DEFINITION  Enterobacter sp. MGH 14 plasmid unnamed4, complete sequence.
ACCESSION   NZ_CP072965
VERSION     NZ_CP072965.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02138554
            Assembly: GCF_018074945.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter sp. MGH 14
  ORGANISM  Enterobacter sp. MGH 14
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Multi-genus dynamics of carbapenem resistance within hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2020) Infectious Disease and Microbiome Program,
            Broad Institute, 415 Main Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.3
            Assembly Name                     :: MGH14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 232x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/14/2023 03:33:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,348
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,100
            CDSs (with protein)               :: 5,100
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 43 of 133
            Pseudo Genes (incomplete)         :: 95 of 133
            Pseudo Genes (internal stop)      :: 25 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            Pseudo Genes (short protein)      :: 1 of 133
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072965.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Enterobacter sp. MGH 14"
                     /mol_type="genomic DNA"
                     /strain="MGH 14"
                     /isolation_source="respiratory"
                     /db_xref="taxon:1329823"
                     /plasmid="unnamed4"
     gene            324..1736
                     /locus_tag="IBG17_RS26515"
     CDS             324..1736
                     /locus_tag="IBG17_RS26515"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032645812.1"
                     /translation="MNEDQDEINNLLAEREWEEFKKSSFGKFFTAKQMKEQELTYLNNK
                     ALDIVNEEDLFEIMSLIPQPFITNENIKKADILSVIYNNFIEETEIPLTLPIFSFFSFL
                     SAFCVKNNITYSMPLSEKKSPVNTWVTVLAPSGSAKTYSLGKIEDLIPIKPDGEKVVEQ
                     NFVKPSGPAKFIEEFSNLPDSRDGKSQYGFWIEDEVAQMFKQVEREGTPLSEIKEYFLK
                     AYDNKKLTRKSKKETIETKNLILVLFFINTFDSYVNNISSESMTDGLMRRFNLVYTEKD
                     KSRDFTDFPIYNEKKITDEILSDRFEDLFFNINPDVNYTFSENAFELYRKYFKIFWHRK
                     YKNILTDKENFYRTYMMQSWKFAVFYHLILKKEGTVIDGECLIYGMKISLLFANSMKKF
                     FDFKNKDISDSKREKQKDTLLKYADFLKTKPDATVRDFCRKFSVKKADAVSILKSLECS
                     GIEHSIFEVVSK"
                     /name="hypothetical protein"
     gene            2008..2313
                     /locus_tag="IBG17_RS26520"
     CDS             2008..2313
                     /locus_tag="IBG17_RS26520"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032645811.1"
                     /translation="MSHEFLIQRNDNSNEQGYFQGYAEGIFYSAFNCQRFNRGVSGSNE
                     QETFTTAETKSALEKVLNSSEIANYPDPDRISSIKDFYFNIVLNAKKKDIFTVRFF"
                     /name="hypothetical protein"
     gene            2313..2663
                     /gene="mobC"
                     /locus_tag="IBG17_RS26525"
     CDS             2313..2663
                     /gene="mobC"
                     /locus_tag="IBG17_RS26525"
                     /inference="COORDINATES: protein motif:HMM:NF017524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_032645810.1"
                     /translation="MPDYKERYQRKNFISLCLSDEELSEVENIADRLNMKRAAAAREIL
                     VTNSKRLKSQIKKNDDAEILFLYSKISNNINQIAKKMNTNLDKFLSGNGEEFSLLIEEI
                     FEDLERLKNNDT"
                     /name="mobC"
     gene            2653..4077
                     /locus_tag="IBG17_RS26530"
     CDS             2653..4077
                     /locus_tag="IBG17_RS26530"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_210954466.1"
                     /translation="MIHKHIGGKKNQRSVKNSLDYLLRTKKPEEQKFVEVLSQTNRSDI
                     ENFNEYIKEKNFKNPFITGVLSFEEKDIDPELKTKIMNEFEEMIFAGIEPENRPPVIWI
                     QHKDKNRLELNYTTFNSLMNKKHFQCYYHLSDKKLFNSFCEKINYENNISSVLDVKEHK
                     ERNTLVNTLNTKIPSDKKAIVEKLQEDILSKIITEEINNREELISFIQSKNIIINRVRE
                     NAISIKFSKEDKPISLKGDIYEQGRDYKTYRTESTIDHRADQQYVENQLRHHRENFDTE
                     LKKRHSRNRTRFKPTQSKDQKNVEYRVKKANDSKNVRSEIPDNNNVPVRNEPDIDFFHN
                     NKTIEDVSNEQNFTNDKTKPREEEPAKFDLSREIETANTQQQFIRSRVEEVRRNQQQFR
                     KSLDRTQERSGVCRRSLHSIIHNIRRYVRTFSIHTRQNERRQKIEHRQKIFLEKLKTQQ
                     EKNNQRSVVRSRFKPY"
                     /name="hypothetical protein"
ORIGIN
        1 aaaactcgct tgttaactgc actaaaccca aaagaagaaa actgactatt ttttcccctc
       61 ccccacttcg tgcaagttta aaaaataatc atattttgct cgtcagaaac ctgtcaggga
      121 ttttgtcaga actttgtcag aactttgtca gaaccgtttt tttacgaaaa acccttataa
      181 atcaatagtt aaaggcattt tgtcagaatg tcggactgtc agaaaaaaag ttaaaaacgt
      241 gtcggagttt ttctttaaaa accttgacaa gttaaattta tattgtatat atattatata
      301 aaataaattt agagggtttt aatatgaacg aagatcaaga cgaaataaac aacttgttag
      361 ctgaaagaga atgggaggaa tttaaaaaat cttcatttgg taaattcttt acggcaaaac
      421 aaatgaaaga acaggaatta acttatttaa acaataaagc attggatatt gtaaacgaag
      481 aagatttatt cgaaataatg tcattaatac cacagccttt tattaccaat gaaaatataa
      541 aaaaggctga tattttgagt gttatataca ataatttcat tgaagaaaca gaaatacctt
      601 taacacttcc gatattttcg ttttttagtt ttttaagtgc tttttgtgta aaaaataata
      661 ttacatattc aatgccttta tctgaaaaaa aatcaccagt taacacatgg gtaacggttt
      721 tagcaccgtc aggatcggca aaaacttact cattgggaaa aatagaggac ttaataccaa
      781 taaaacctga tggcgaaaaa gttgttgagc aaaattttgt taagccgtca ggcccagcaa
      841 aatttattga agagttttca aatcttccag attccagaga cggaaaatct caatatggct
      901 tttggattga ggacgaagta gctcaaatgt tcaaacaggt tgaaagggaa ggaaccccgc
      961 tttcagaaat aaaagaatat ttcttgaaag cctatgacaa taaaaaatta acaagaaaaa
     1021 gcaaaaaaga gacaatagaa actaaaaatc ttatattggt tttatttttc ataaacactt
     1081 ttgatagtta tgtaaataat atatcttccg aatctatgac agatggttta atgagaagat
     1141 ttaatcttgt ttatacagaa aaagataaga gccgagattt tactgatttt cctatataca
     1201 acgagaaaaa aatcacagac gaaattttat ctgataggtt tgaagattta tttttcaata
     1261 tcaatccaga tgttaattat accttttcag aaaacgcttt tgaactttac agaaaatatt
     1321 tcaagatttt ttggcataga aaatataaaa atattcttac cgataaagaa aatttttaca
     1381 gaacatatat gatgcaaagc tggaaatttg cagttttcta tcacttgatt ttgaaaaaag
     1441 aaggaacagt gatcgatggt gaatgcttaa tatatggaat gaaaatttct ttgttatttg
     1501 ccaacagtat gaaaaagttt tttgatttta aaaataaaga tatatcagac tcaaaaagag
     1561 aaaaacagaa agatacttta ttgaaatatg ctgattttct caaaacaaaa ccagatgcga
     1621 cagttaggga tttttgcaga aaatttagtg ttaaaaaggc agatgctgtt tcaattttga
     1681 aaagtcttga atgctcagga atagaacatt caatatttga agttgtgagc aaataacttg
     1741 ccgatttaaa ttttgtattt atatttaatt ttctttcggc aagttcaacc cctcaaaaat
     1801 aataaagccc ttacgggctt ttttattttc tcacggtttt ataaaaggtc aaattatatt
     1861 cagttttgcc cttgacttaa ggttttaatt tgttatctat gtaatagata gcaagttaga
     1921 gtaatacttc gtattccttt acttgtgagg ggttccccct tcaatcccct aaaaaccaaa
     1981 atcaaaacct aaaccaagga gcaaaatatg agtcacgagt ttttaatcca gagaaatgac
     2041 aacagcaatg aacaaggcta tttccaagga tatgccgaag gcatattcta ttcagccttt
     2101 aattgccaaa ggttcaatag aggcgtttct ggttctaacg aacaagaaac tttcaccaca
     2161 gcagaaacaa aatcagcatt agaaaaagtc ttaaattcta gcgagatagc aaactatccc
     2221 gatcctgatc gtataagtag tattaaagat ttttatttca atattgtatt aaatgctaaa
     2281 aagaaagata tttttacagt gagatttttc taatgccaga ttacaaagag aggtatcaga
     2341 gaaaaaattt tatctctctt tgtttgagtg atgaagaatt atcggaggtt gaaaatatcg
     2401 ctgataggtt aaatatgaaa cgagccgccg cagctcgtga aatattagtc acaaactcaa
     2461 agagactaaa atcacaaata aagaaaaatg atgatgctga aatattgttt ttatattcta
     2521 aaatctcaaa caatataaat cagatcgcaa aaaagatgaa tactaatttg gataaatttt
     2581 tatcaggaaa cggagaagaa ttttcactac tcattgaaga aatattcgaa gatttagaga
     2641 ggttaaaaaa taatgataca taaacatatc ggaggaaaga aaaatcagcg aagtgttaaa
     2701 aattcacttg attatttatt aagaactaaa aaaccagaag aacagaaatt tgttgaggtt
     2761 ttatctcaaa caaacagatc tgatattgaa aatttcaacg aatacataaa agaaaaaaac
     2821 ttcaaaaatc ccttcataac tggtgtttta tcttttgaag aaaaagacat cgatccagaa
     2881 ctaaaaacaa aaataatgaa tgaattcgaa gaaatgattt ttgcaggaat agagcctgaa
     2941 aaccgcccac cagtgatctg gatacaacac aaagataaaa acaggcttga attaaactat
     3001 acgactttta attctttaat gaataaaaaa cattttcaat gttattatca tttatctgat
     3061 aaaaaattat tcaactcatt ttgtgaaaaa ataaattatg aaaataatat tagttctgtt
     3121 ttagatgtta aagaacacaa agaaagaaac actcttgtaa atactttaaa tactaaaata
     3181 ccatcagata aaaaagccat tgttgaaaaa ttacaggaag atattttatc gaaaataatc
     3241 acagaagaaa taaacaacag agaagaatta atatcattca ttcaatctaa aaacataata
     3301 ataaatagag ttagagaaaa tgctatatca ataaagtttt caaaagaaga taaaccaata
     3361 tcactgaaag gagatatata tgaacagggc agagattaca aaacttatag aacagaatca
     3421 acaatcgatc acagagcaga tcagcagtat gtcgaaaacc aactcagaca ccatagagaa
     3481 aattttgaca cagagcttaa aaaaagacac agcagaaata gaactcgttt taagccaaca
     3541 cagagcaaag accagaaaaa tgttgaatac agagttaaaa aagcaaatga cagtaaaaat
     3601 gttagatctg aaataccaga caataataat gttcctgtta ggaatgaacc tgatattgat
     3661 ttttttcata ataataaaac tatcgaggat gtaagcaatg agcaaaattt cacaaatgat
     3721 aaaacaaaac caagagaaga agaaccagca aaatttgatc tatcaagaga aattgaaaca
     3781 gctaatacac aacaacaatt cattcgttca agagttgaag aggttagaag aaatcaacag
     3841 caattcagaa agtcgcttga tagaactcag gaacgatctg gagtatgtag aagatctcta
     3901 cattcaatta ttcacaatat tagaagatac gtcagaacgt tttcaatcca tacaagacag
     3961 aatgaaagaa gacaaaaaat cgaacacaga caaaaaatct tccttgaaaa gctcaaaacc
     4021 cagcaagaaa aaaacaatca aagaagtgtc gtcagaagtc gatttaaacc gtattagaac
     4081 cgttctgaat actcttctaa cccactacga caaaacctta accgtgaaca agtgagaacc
     4141 agccagaggc tggtttttgc ttgtgagggg ttgaacaagc gaaaaccaac atacatagat
     4201 cactggtgt
//
