LOCUS       NZ_CP072964             4866 bp    DNA     circular CON 14-AUG-2023
DEFINITION  Enterobacter sp. MGH 14 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP072964
VERSION     NZ_CP072964.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02138554
            Assembly: GCF_018074945.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter sp. MGH 14
  ORGANISM  Enterobacter sp. MGH 14
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Multi-genus dynamics of carbapenem resistance within hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2020) Infectious Disease and Microbiome Program,
            Broad Institute, 415 Main Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.3
            Assembly Name                     :: MGH14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 232x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/14/2023 03:33:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,348
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,100
            CDSs (with protein)               :: 5,100
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 43 of 133
            Pseudo Genes (incomplete)         :: 95 of 133
            Pseudo Genes (internal stop)      :: 25 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            Pseudo Genes (short protein)      :: 1 of 133
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072964.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Enterobacter sp. MGH 14"
                     /mol_type="genomic DNA"
                     /strain="MGH 14"
                     /isolation_source="respiratory"
                     /db_xref="taxon:1329823"
                     /plasmid="unnamed3"
     gene            complement(join(4817..4866,1..205))
                     /locus_tag="IBG17_RS26475"
     CDS             complement(join(4817..4866,1..205))
                     /locus_tag="IBG17_RS26475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_247687638.1"
                     /translation="MKLNVLARFFYYIEQDKDIPFSEINIDERDLCYFVANRYITENKA
                     DELIEALIIENDDDYIRATEDYIIMRNKKCQQQTEDEDV"
                     /name="hypothetical protein"
     gene            complement(207..473)
                     /locus_tag="IBG17_RS26480"
     CDS             complement(207..473)
                     /locus_tag="IBG17_RS26480"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_155858589.1"
                     /translation="MIDEMTQYRIRRFTQSQKSLLLKMIKERQLRDLEDNIKDILKDID
                     VSYSLGITKKASRKEEMIACREVHHIFSILTKRDINNDYRNRI"
                     /name="hypothetical protein"
     gene            complement(480..746)
                     /locus_tag="IBG17_RS26485"
     CDS             complement(480..746)
                     /locus_tag="IBG17_RS26485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032645794.1"
                     /translation="MNIVLSVSKCLYINDLDRLLPCDRFFVHLDYRDISKKESFEMNRH
                     DKETYKLIFDKLVGNDVMLAIDEDIYSEVVENLLYYSNKTYGD"
                     /name="hypothetical protein"
     gene            complement(1360..1584)
                     /locus_tag="IBG17_RS26490"
     CDS             complement(1360..1584)
                     /locus_tag="IBG17_RS26490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032645795.1"
                     /translation="MLEQKDKKRRLGFMIGKSNATLPADEQTEAELDQEVLKLFYPNQY
                     TKDTIERSEAGTDVFTASDSDDLFKKLKL"
                     /name="hypothetical protein"
     gene            1875..2726
                     /locus_tag="IBG17_RS26495"
     CDS             1875..2726
                     /locus_tag="IBG17_RS26495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_032645796.1"
                     /translation="MFKIENNKDILKSQIPLKPYATNDLDFGLTIQNKNKALDMLYLQV
                     NHPLYLHTMVFDLDKENCFYEFENAHLPIPSFITKSPDSGRCHYGYMLNAPITSTEKSR
                     QKPVKFARALYYNMATRLGADLGYAGLITKNPYSPHWSPFWSGADLYELNDLADCFDDL
                     EEPKKRENTGFAFGRNVEMFDTIRQWAYKNVLKYQSESSFNDFHNELLLKCQMHNAYIN
                     TDDLLPYNEIKATAKSIAKFCWKEFSEEKLNKIQSKKQSYRGKKNKGIVKSKTRKFLEA
                     IK"
                     /name="replication initiation protein"
     gene            2723..2992
                     /locus_tag="IBG17_RS26500"
     CDS             2723..2992
                     /locus_tag="IBG17_RS26500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032645797.1"
                     /translation="MKVKNRKRELTAKEYAEQYEISVRTVKRYFSQDREDYEKEAKQRR
                     LKAFVLRESGMKWSDVGIALGTTKHGAIALYKRYQQIDAPIKEA"
                     /name="hypothetical protein"
     gene            complement(3001..4476)
                     /locus_tag="IBG17_RS26505"
     CDS             complement(3001..4476)
                     /locus_tag="IBG17_RS26505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_210954465.1"
                     /translation="MKGNVAKTGRSFKNRVDYILKDDHSFICSNMSADKNNVSDLTDEF
                     KTVSSFRQDIKKPVFHAYLSLPKNEHLTDEKWQEIAKDYLKEMNIDIEKHQYICVRHND
                     TDKEHIHIVANRVGLDGSVWLGQHSAFNTIAACERLEVKHSLTITQSLKGQKSDVSAPT
                     KNEIEQALRKGEKPARLVLQNALQAALVGKPDLSTFVERLQAVGIEPRFNVASTGNVAG
                     CSFSVGDVAFKGSQLGKKFSWNTIKGKVKYDKNRDDELVRSFSARKDDEPNSIGRSLVE
                     SNINDSRAVDGIDDDIIRISQHSIKSDTTDTNKSEQLIRSNNNSFKLSEKYNRPTNKKI
                     GRATHKAAQILNRVKKQYNKEPIKTYNKNGLNSWRDKSDSGSISRILDHAETATTATNN
                     FRLKGRRNINSRATKIEEIRRRLGIEKPVIQPKKIVKEEIKKKPLGFSMDENRFKSKLK
                     EAIKKEKPKNETTPTQLQQEDDQHLKPRGFKPR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4473..4850)
                     /gene="mobC"
                     /locus_tag="IBG17_RS26510"
     CDS             complement(4473..4850)
                     /gene="mobC"
                     /locus_tag="IBG17_RS26510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_032645791.1"
                     /translation="MPTTDRRRGRINLNADERRDISIQFRVNKAEFEYLKSLVEASGLE
                     RGHYIRKEILNSEPVIKRKMPEVNQKTYMQLSAIGNNINQAVKKLNGNEYFDKNEVDKL
                     NFNIKQVSRRIIEINKDLGYI"
                     /name="mobC"
ORIGIN
        1 taatgtaatc ctctgttgct cttatgtagt cgtcatcatt ttcaataatc aatgcttcaa
       61 taagttcatc agctttattt tctgtaatgt atctatttgc tacaaaataa cataaatccc
      121 gctcatcgat atttatttca ctaaatggta tatctttatc ttgttctatg tagtaaaaaa
      181 aacgtgctaa tacattaagt ttcatttcat attctatttc tataatcatt attgatgtct
      241 ctttttgtta atatactaaa gatgtgatga acttcacgac aagcgatcat ttcttctttt
      301 cttgatgctt tctttgttat gcctaatgaa taactaacat caatatcttt taaaatatct
      361 tttatgttgt cttctaaatc tcttagctgc ctttctttta tcatctttag tagcaagctt
      421 ttttgacttt gtgtaaagcg tcttattctg tattgtgtca tttcatctat catcttatat
      481 taatctccat aagtcttatt tgaataataa agtaagtttt cgacaacttc gctgtagatg
      541 tcttcatcaa tagcaagcat tacgtcatta cctacaagtt tatcaaagat aagtttgtat
      601 gtttctttgt cgtgtctatt catctcaaat gactcttttt tgctaatatc tctataatct
      661 aaatgaacaa aaaaacgatc acacggaagt aagcgatcta aatcgtttat gtataaacat
      721 tttgatactg ataaaactat attcatattt attccttttt gttttgatgt aaaactattt
      781 accgcctccc ctttgacaag gggaataggc ggaagggtag atcttttaac aaccctccga
      841 acgaagtgag agggggcaac ctgaaaggtt gggtataata ccggctacgg gattacacct
      901 tgcctcagtt tccgaaaagc taaaaataaa gctgaactca acctatattt aaataaataa
      961 ccgatctaaa aagtttgtca atatagcggc tacataaata aatacactgc acgacaaata
     1021 aaatgcaaat aaaaataggg taaataatat attgtcttga tgggtagctg tgcgtaataa
     1081 gtagctttgc gtaaaaaatg ccgctgctac gtgaaatctg gaaaagttcg tgaccacttg
     1141 gtaggcagat tttaagaaat ggggtttgat cggcggcgac agaacagtag aaaacttgct
     1201 tttctgctgt tgctggcgtc tatggttttg aaatggctat aaaatcaaca ctatagatta
     1261 gtataaatca gtatatactg catcgagggt tggccttctc tcaccgttcc gggtcggttt
     1321 gttctcaact ggtgaagatg gccagctctc ccctaccctc tacagcttca attttttgaa
     1381 caggtcatca gaatcggagg ccgtgaaaac gtctgtaccg gcttctgagc gttctatggt
     1441 gtctttggtg tattggttag gataaaacag cttcaggact tcctgatcaa gctctgcttc
     1501 tgtttgttcg tctgctggca atgttgcatt gctcttacca atcataaaac ctaaccgccg
     1561 tttcttgtcc ttctgttcga gcatgattta cctccccagc acagccagat cctgcatatg
     1621 aagtttagta tacgttactt tactctgcgg tagccttcgg cctgtgcttc gctgtggtgt
     1681 agttttccaa aaactccgtc taatacctga tattggttaa tggcacacat aacaactttt
     1741 tttgacggat ctattgacaa gtttttttgg tatagtaaaa ctgtaaaaga ttataaataa
     1801 aagaaaagcc cgcgttctct gattagtttg gcgaccagtg agagaacggg gcattacaga
     1861 aaggatactg ctatatgttc aagatagaaa ataacaaaga tatattaaaa agtcaaatac
     1921 ctttaaagcc atatgctaca aatgatctcg attttggatt aactattcaa aataagaata
     1981 aagctttaga tatgctttat ttacaagtaa atcacccgct ttatcttcat acaatggtat
     2041 ttgatttaga caaagaaaac tgcttttatg aatttgaaaa tgcccatttg cctataccat
     2101 cttttataac taaaagccct gatagtggac gttgccatta tggttatatg cttaatgctc
     2161 ctataacatc aacagagaaa agcagacaga agcccgtaaa gtttgccaga gcattatatt
     2221 acaatatggc tactcgttta ggtgctgatc tggggtatgc tggactgatt actaaaaatc
     2281 catacagccc gcattggtcg ccgttctggt caggtgctga tttatatgag ttaaatgatt
     2341 tagctgattg ctttgatgat ttagaagaac caaaaaaacg agaaaatacg gggtttgctt
     2401 ttgggcgtaa tgttgaaatg ttcgacacca taaggcaatg ggcttataaa aatgtgctaa
     2461 aatatcagag tgaaagctca tttaatgatt ttcacaatga actattgcta aagtgtcaaa
     2521 tgcataatgc atatataaat actgatgatt tactgccata taatgagata aaagccacag
     2581 caaaaagtat agcaaagttt tgctggaaag agtttagcga agaaaaactc aacaaaatac
     2641 agagtaaaaa acagtcttac cgtggaaaga aaaacaaagg aatagtaaaa agtaaaactc
     2701 gtaagttttt ggaggcgata aaatgaaagt taaaaataga aaaagagaac ttacagcaaa
     2761 agagtatgct gaacagtatg agattagcgt taggacagtt aaacgatatt tttctcaaga
     2821 tcgtgaagac tatgagaaag aagcaaaaca aagacgctta aaagcttttg tattacgtga
     2881 aagcggtatg aaatggtctg atgttggtat tgctcttgga actactaaac acggtgccat
     2941 agctctttat aaacgttatc aacaaataga cgcaccaata aaagaggctt aaagcctcct
     3001 ttatcgcggt ttaaaacctc ttggttttaa atgctgatcg tcttcctgct gtagttgtgt
     3061 tggtgttgtt tcattttttg gtttttcttt tttaatagct tcttttaact tcgatttaaa
     3121 tctattttca tccatagaaa aacctaaagg ctttttcttt atttcttctt taactatttt
     3181 ctttggctga ataacaggtt tttctattcc aagcctacgc cttatttctt cgatcttagt
     3241 tgctctacta ttgatgttgc gacggccttt gagtctaaag ttattcgtgg ctgttgtggc
     3301 tgtttctgca tgatcaagta tcctactgat agaaccgcta tcagatttat cacgccaact
     3361 attgaggcca tttttattat aagttttgat tggttcttta ttgtattgtt ttttaactct
     3421 gttgagtatt tgtgcagctt tatgtgttgc tcttccaatc tttttgtttg ttggtctatt
     3481 atatttttcg ctgagtttaa agctgttgtt gttgcttcta atgagttgtt cactcttgtt
     3541 agtgtctgtt gtgtctgatt taatgctgtg ctgactgatt cggataatat catcatcgat
     3601 tccgtcaacg gctctactgt cattgatatt tgattcaaca agtgatcgtc caatactatt
     3661 tggttcatca tcttttcttg ctgagaagct cctaacgagt tcatcatctc tatttttgtc
     3721 atatttcacc ttgcctttaa tagtgttcca actaaatttt ttacctaact gagatccctt
     3781 gaatgccaca tctcccacac tgaaagaaca acccgccacg ttgccagtgc tggcaacatt
     3841 gaaccgaggt tcaatgccta cggcctgcag acgttctacg aacgtggaca aatcaggctt
     3901 accgactaaa gccgcttgta gtgcgttctg tagcactaaa cgagctggtt tctcaccttt
     3961 gcgtagggct tgctcgatct cgttcttggt tggtgctgat acgtctgatt tttggccttt
     4021 gagactttgt gtaatggtta agctgtgctt tacttctaaa cgttcacaag ctgcgattgt
     4081 attaaatgct gagtgctgcc caagccatac cgaaccatct aaaccgactc tatttgctac
     4141 tatatggata tgttctttat ctgtatcgtt atgacgcaca caaatatatt gatgtttttc
     4201 tatatcaata ttcatttctt tcaaatagtc ttttgctatc tcttgccatt tctcatctgt
     4261 taaatgttca ttttttggaa gagataaata tgcatgaaac actggttttt ttatatcttg
     4321 tctaaagctg cttacagtct tgaactcatc agttaaatca ctaacattat ttttatctgc
     4381 tgacatattg ctacaaataa aactgtgatc atcttttaat atgtaatcaa ctctattttt
     4441 aaatgatctt cccgttttcg ctacattccc tttcatatat accccaagtc tttatttatt
     4501 tctattatcc ttctgcttac ttgctttata ttaaagttga gtttatcaac ttcattttta
     4561 tcaaaatact cattgccatt taacttttta acagcctgat ttatattgtt ccctatagct
     4621 gaaagctgca tatatgtttt ttgatttact tccggcattt tccttttaat aactggctca
     4681 gagtttaaaa tctcttttcg gatgtaatga cctctctcaa ggccggaggc ttccactaat
     4741 gattttaaat attcaaactc tgctttgttt actctaaact gtatagatat atctctgcgt
     4801 tcgtcagcat ttaaatttat acgtcctcgt cttctgtctg ttgttggcat tttttattcc
     4861 tcataa
//
