LOCUS       NZ_CP072960             2264 bp    DNA     circular CON 14-AUG-2023
DEFINITION  Enterobacter sp. MGH 3 plasmid unnamed7, complete sequence.
ACCESSION   NZ_CP072960
VERSION     NZ_CP072960.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02138543
            Assembly: GCF_018075145.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter sp. MGH 3
  ORGANISM  Enterobacter sp. MGH 3
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Multi-genus dynamics of carbapenem resistance within hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2020) Infectious Disease and Microbiome Program,
            Broad Institute, 415 Main Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.3
            Assembly Name                     :: MGH3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 183x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/14/2023 03:14:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,093
            CDSs (total)                      :: 4,978
            Genes (coding)                    :: 4,853
            CDSs (with protein)               :: 4,853
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 39 of 125
            Pseudo Genes (incomplete)         :: 89 of 125
            Pseudo Genes (internal stop)      :: 22 of 125
            Pseudo Genes (multiple problems)  :: 22 of 125
            Pseudo Genes (short protein)      :: 1 of 125
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072960.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Enterobacter sp. MGH 3"
                     /mol_type="genomic DNA"
                     /strain="MGH 3"
                     /isolation_source="respiratory"
                     /db_xref="taxon:1329812"
                     /plasmid="unnamed7"
     gene            359..625
                     /locus_tag="IBG19_RS25345"
     CDS             359..625
                     /locus_tag="IBG19_RS25345"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223173001.1"
                     /translation="MKRSQLTLIDGCFMFGFSPGELMNLCPDERLLFVRMISAMLRRSG
                     GDAGAVMFEAYRHIVSDTNQARRSYMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            complement(673..861)
                     /locus_tag="IBG19_RS25350"
     CDS             complement(673..861)
                     /locus_tag="IBG19_RS25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1113..1430)
                     /locus_tag="IBG19_RS25355"
     CDS             complement(1113..1430)
                     /locus_tag="IBG19_RS25355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000718044.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAANVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEATGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAV
                     Q"
                     /name="hypothetical protein"
     Typing          43..215
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.954"
                     /coverage="100.0"
                     /evalue="2.04e-62"
     Typing          complement(1837..2252)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.411"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 gcaccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaat
       61 atctcctgtc tcatatattc cgcttacgct ctgctgctgc attttcctcc tgccacatga
      121 agcacttcac tgattcccgc acccgtgcct acatagtaag ccagtataca ctccgctagc
      181 gctacgtgac tggttcaggg ctgcgccccg acacccgcta aaagcactga cgcgcctgcg
      241 gcttgcccaa ccccgcgccg tccgggaaag aatttcccga tcgacccgac gtttctgttt
      301 acctgtgatg ttaacgtttg gatctttttc ccgggtttac ctattttaat gaagcgtaat
      361 gaagcgttca cagctgacgt taatagatgg gtgcttcatg tttggttttt caccaggaga
      421 acttatgaac ctttgccccg atgaacgtct gctctttgtg cggatgattt ccgctatgct
      481 tcgccgttcg ggcggtgatg cgggtgctgt tatgtttgag gcgtatcgcc atattgtgtc
      541 cgataccaat caagctcgtc gttcctacat gctcgacctg ctcgaaagcg tcaggcatga
      601 ctatgtacac ggtggatata catagttgcc ctggatacgc ttttcgtcta agtgagcgtt
      661 cttttcgtct ctttattcgc cgtcctgtat accgaagcgt gcgctcagct tctgataaag
      721 cgcttctgcc tgttcgtgca ttcgctcaca gtcggctgcc tcgtcatcaa gatcgagcac
      781 gtcgagtttt tcgagcagca gcagcgactg cgcccggata aattttgcca tgttcagtgc
      841 ggctgattcc tgttttgtca tacggctttt tcccggtttt atggtgatcg cagactgaaa
      901 tcttacttca ttcacttgag tgcatagcca tgcaggtacg cagaaatcat caggggcgtt
      961 ttttctcgat atgggcacag aaaggtgtat gtttcaggcc ggagtcctca gaaacgccct
     1021 gagggcgcct gaggcggtgc gggcaacgga tgttatggta aatccagctg attggcaggt
     1081 acaaaaaaag ccccgcaggg cttttttgta ccttattgaa ctgcacactt tgcagccttc
     1141 agggcttcca gctgatttag ccggttagct gcttttttgc gggcttcgcc ttttgccatg
     1201 ccatttccta tcccaaaatc acccaggaaa cctaatacgg tgcgcccgtc aaattcgccg
     1261 gttgcctcaa tttcttgttg tacactgcgc gtttttgcga tttccagagc taccgctttg
     1321 caatccattg cggaagattc tggaccggtg acgttcgcgg cctgtggata ttgctttgtt
     1381 gcgcatcctg ccatgatcag cgctgccgat aggatgaata tttttttcaa aattatcctt
     1441 attttattta ggcatagtta agagataccc attttatcgg caggaaatga gggtttttta
     1501 gttgaatttt gggaggggca aggttccata ttggtcgagg attctggagc tgaccggcaa
     1561 cggccagcgc ggctatgggc catgcggtgg ttgagcatag aaaggatctt cttgagttcc
     1621 tttttttcta cgcgtaatct cttgctctga aaacgaaaaa accacctggg gaggtggttt
     1681 ttcgaaggtt aagtcagttg gggaactgct taacctggta actggcttca gcagagcgca
     1741 gataccaaat actgtccttc cagtgtagcc gtagttaggc caccacttca agaactctat
     1801 agcaccatct acattaactc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     1861 ctttgtcgtg tctttccggg ttggactcaa gacgatagtt accggataag gcgcagcagt
     1921 cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc ttcccggaac
     1981 cgagtgtcag gcgtggaatg agaaaccgcg gccataacag cggaatgaca ccggtacacc
     2041 gaaaggcagg aacaggagag cgcacgaggg agccaccagg gggaaacgcc tggtatcttt
     2101 atagtcctgt cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgcttgtcag
     2161 gggggcggag cctatggaaa aacggcttcg ccgcggcctt atcgtttccc tgttaagttt
     2221 cttcctggca tctcccagga aatttccgcc ccattcgtaa gccc
//
