LOCUS       NZ_CP072959             2495 bp    DNA     circular CON 14-AUG-2023
DEFINITION  Enterobacter sp. MGH 3 plasmid unnamed6, complete sequence.
ACCESSION   NZ_CP072959
VERSION     NZ_CP072959.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02138543
            Assembly: GCF_018075145.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter sp. MGH 3
  ORGANISM  Enterobacter sp. MGH 3
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Multi-genus dynamics of carbapenem resistance within hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2020) Infectious Disease and Microbiome Program,
            Broad Institute, 415 Main Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.3
            Assembly Name                     :: MGH3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 183x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/14/2023 03:14:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,093
            CDSs (total)                      :: 4,978
            Genes (coding)                    :: 4,853
            CDSs (with protein)               :: 4,853
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 39 of 125
            Pseudo Genes (incomplete)         :: 89 of 125
            Pseudo Genes (internal stop)      :: 22 of 125
            Pseudo Genes (multiple problems)  :: 22 of 125
            Pseudo Genes (short protein)      :: 1 of 125
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072959.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter sp. MGH 3"
                     /mol_type="genomic DNA"
                     /strain="MGH 3"
                     /isolation_source="respiratory"
                     /db_xref="taxon:1329812"
                     /plasmid="unnamed6"
     gene            339..524
                     /locus_tag="IBG19_RS25330"
     CDS             339..524
                     /locus_tag="IBG19_RS25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(815..1033)
                     /locus_tag="IBG19_RS25335"
     CDS             complement(815..1033)
                     /locus_tag="IBG19_RS25335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            2241..2468
                     /locus_tag="IBG19_RS25340"
     CDS             2241..2468
                     /locus_tag="IBG19_RS25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          complement(572..659)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          1298..1826
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.411"
                     /coverage="88.0"
                     /evalue="3.17e-180"
ORIGIN
        1 gtggcctttt tctttacctg tggttctccg ccaaaatccc agcaaattgc atcaccaaag
       61 ctaaaagctt tcagggttgt ctaaaaagca caagacataa gaggaagtgc ggtattttat
      121 aatcaagccc ccaagaattt accataacat ccgttgcccg caccgcctga gacgcgttca
      181 gcgcgttcct gacgaaacca tgacaaaaac cacaacaaac caccccggaa cccgtcagaa
      241 acgcgcctgt taaattttaa cggcatgcat gactatgcac cagaatgacg ccatgctctt
      301 ttcacgcaaa aatcatcacc agacggggaa aatcaccagt gaccagacag gaatccgccg
      361 ccctcaatat ggccaaattt atccgcgcac agacacttct cctccttgag cggctcgagc
      421 agatggatct ggatgaggct gccggctgct gtgagcacct gcacgatcag gccgaagcgc
      481 tttacgccat gctgaacgca cagataggcg aggaaaatgc gtgaaaatcg gtgaacgggt
      541 gcgcaattca gtgcgcggcc gtgaggcgat ggcggggtgt cggggcgcag ccctgaccag
      601 ggtatttgtg atgccggcgc gtgcgcggta ttacaaatgc acatcctgtc ccggaacgga
      661 caccgggaaa cagcaaaaaa aaccgggcgg cacgcccgga actcaatcaa gttagattag
      721 attactctca ctcgtccata acagcatcat ggaacgacga ccaccgtccg tgacggccgc
      781 ctcgtttaag tatggacaga aatacagaaa atgctcagga cgaaatgtaa tgaatgcgaa
      841 cggattcaag aaattcgagc atgacagtcc ttacggccgg ttcggtttca gacaaaatct
      901 gccggtatgc atccagcatc atggctccgg catcccctcc ggcacgccgt agccagaccg
      961 aaacaacgga cacaagcagg tgtcgctcat catcactaag agtcatcagg gctccggaag
     1021 aaaaaccaaa catggcttac ctactgttaa cggcaacgca gaagaaaggt ttacagggta
     1081 gcgggcaggg atgaaaaaaa aaaaattgca gtcaggccgg aacggctgct gaaccggtac
     1141 aggcgggttc cgggcgcagc gccggaccag tcacggagca ctagcggagt gtatactggc
     1201 ttaagatgta gcatggcagt aacacatagc attaatgcca cataagacag gcgcagaaag
     1261 tgaaagaaaa cgcgtcagga gaaaatgtga tacagaatat attccgcttc ctcgctcact
     1321 gactcgctcc gctcggtcgt tcggctgcgg cgagcgggac gggcttacgg atggatcaga
     1381 aatttcctgg gggacgccag gaagatactt aacgaagaaa gcataaggcc gcgccaaagc
     1441 cgtttttcca taggctccgc ccccctgaca agcatcacag aatctgacgc tcaaatcagt
     1501 ggtggcgaaa cccgatgcgg cttaaagata ccaggcgttt ccccctggtg gctccctcgt
     1561 gctcccccgc attcctgcct ttcggtttac cggtgtcact ccgctgttat ggccgcggtt
     1621 tctcattcca cgcctgacac tcagttccgg gaaggcagtt cgctccagga tggactgtat
     1681 gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc aacttgagtc
     1741 caacccggaa agacacgact aatcgccatt ggcaacggcc attggcaaac aggatgtgcg
     1801 agagatgtct caagagttct tgaagtggcg gccttagtga ggctacactg aaaggacaaa
     1861 tttggtgaca tgtctcgcac aagactgtta ccacggttag gggatctgcc aggattacct
     1921 aaccttcgaa aaaccacctg ccagggtggt tttttcgttt acagggcaag agattacgcg
     1981 cggaaaaaaa ggatctcaag aagatccttt ataaattaga catccaacct cacaaagagg
     2041 aatacttaaa tcatcttggt atatgtatcg gccggattac atctcatata agcctcctcc
     2101 ccgcctgaca ccacactttg accatagcac ctcgcgccta actcgatata cagcccacca
     2161 tcaagcctct tttcacataa aaaatgacct atagttagat ggttagtgac caaaactgat
     2221 atggataaaa ggagctggct ttgaaaaaga tttttgtttc agtgttcgca gcggcagttg
     2281 cgctttcagc cctcaccggc tgcacgcgca caagctatgc catacacacc aatgacggtc
     2341 gcactattgt cagcgacggc aaacctaaag aatcagactc aggcctgctt ggctatacag
     2401 acgcgaatgg tgtcaaacag cagatcaata aaaccgacgt taaagaagtc tccgaaattc
     2461 cgcactgagc atcttctccg aaaaaggccg ctaat
//
