LOCUS       NZ_CP072958             2811 bp    DNA     circular CON 14-AUG-2023
DEFINITION  Enterobacter sp. MGH 3 plasmid unnamed5, complete sequence.
ACCESSION   NZ_CP072958
VERSION     NZ_CP072958.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02138543
            Assembly: GCF_018075145.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter sp. MGH 3
  ORGANISM  Enterobacter sp. MGH 3
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Multi-genus dynamics of carbapenem resistance within hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2020) Infectious Disease and Microbiome Program,
            Broad Institute, 415 Main Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.3
            Assembly Name                     :: MGH3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 183x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/14/2023 03:14:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,093
            CDSs (total)                      :: 4,978
            Genes (coding)                    :: 4,853
            CDSs (with protein)               :: 4,853
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 39 of 125
            Pseudo Genes (incomplete)         :: 89 of 125
            Pseudo Genes (internal stop)      :: 22 of 125
            Pseudo Genes (multiple problems)  :: 22 of 125
            Pseudo Genes (short protein)      :: 1 of 125
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072958.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Enterobacter sp. MGH 3"
                     /mol_type="genomic DNA"
                     /strain="MGH 3"
                     /isolation_source="respiratory"
                     /db_xref="taxon:1329812"
                     /plasmid="unnamed5"
     gene            87..1370
                     /locus_tag="IBG19_RS25655"
     CDS             87..1370
                     /locus_tag="IBG19_RS25655"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_227524674.1"
                     /translation="MKSQFMHVELYSREEPAKKTINKSKNINHKSDVRTTTVNGVLSEM
                     KREEGFTSHLETIAAPEVLYGSIDALERSIERYEAEFKTTDKNGKEKKLRKDACILLAG
                     VVSLDRADEEIWDEYKTKAIEYLKNKYGENLKCVVEHTDETNPHFHFYVVGNKDQDLNL
                     LHDGKLAVSKLAKEDKKKLHQTAYTEAMIKFQDDFYVQVSNKFGLSRTGEEPRERYKSR
                     PDYLRFKKRQETALNILANLEEFEEKELKKAKQAGFNVGIKNFDETNFFRKLDWRVKRI
                     KKEKDNEIKKLSDENDNLNIDKSNLQHELDMSKIELSDAYSQRNEASRKNSVYIDKVNA
                     LKKENKLLLPYKDQFDLKLKQEKEPLQAEVKELTSENTNLKNENTKLKKENLDFKEFIL
                     GVKNYYGSKFQEWYEEVFNPKTKTKPKF"
                     /name="plasmid recombination protein"
     gene            2055..2654
                     /locus_tag="IBG19_RS25325"
     CDS             2055..2654
                     /locus_tag="IBG19_RS25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="WP_001029633.1"
                     /translation="MAKAKVLINDTDEAVIKNALDVEDMNEVNINVTRKKKTGFLGTYS
                     IVFHDSVKTLSKMNLKPNAYKIVMYLFGIIEMGNILVNFSQKKIADDLGLQPSNVSRAF
                     KELFDKGVLIKDSEDGHVYLNSNLCTMGVPKNFDQKRMKSLQKSNIETEDFKNQINLFT
                     PTNTAKIKTNDKKVIKKKEKENVLEFPMQNDWVDIE"
                     /name="Crp/Fnr family transcriptional regulator"
ORIGIN
        1 tatagtcggt tcctatagct attgacagaa aaacaataaa tgttaattac atatataagg
       61 aaaattaaaa tataggagaa tttaaaatga aatcacaatt tatgcatgta gaactctaca
      121 gcagagaaga accagctaaa aagacaataa ataaaagtaa aaatataaat cataaatctg
      181 atgttagaac aacaaccgta aacggtgtgt tgtcagaaat gaaaagggag gagggtttta
      241 catcccattt agaaaccata gctgcaccgg aggtgctcta tgggtctata gatgccttag
      301 aacgctctat agagcgttac gaggccgaat ttaagactac agacaagaac gggaaggaga
      361 agaagttacg caaagatgca tgcatcttgc tagctggtgt ggtttctcta gatcgagctg
      421 atgaagaaat ttgggatgaa tataagacca aagcaattga atatttaaaa aataaatatg
      481 gtgaaaattt aaaatgtgtt gttgagcata cagacgaaac caacccacat tttcattttt
      541 acgtcgttgg aaacaaggat caagatttaa atttgcttca tgatggaaag ctcgcagtat
      601 ctaaattagc aaaagaagat aagaaaaaat tacatcaaac agcttacaca gaagctatga
      661 tcaaatttca agatgatttt tatgtacagg tatcaaataa atttggattg agcagaacgg
      721 gcgaggagcc gcgagaacgc tataaatcac gccctgatta cttgagattt aaaaaaagac
      781 aagaaacagc attaaatata ttggccaatt tagaagagtt tgaagaaaaa gaattaaaaa
      841 aagcaaaaca agctggcttt aatgttggta ttaaaaattt cgatgaaact aattttttta
      901 ggaaactcga ttggagagta aaaagaataa aaaaagaaaa agacaatgag attaaaaaat
      961 tatcggatga aaacgataat ttaaatatag ataagtcaaa tttacagcat gaattagata
     1021 tgagtaaaat tgagctttca gatgcatata gtcaaaggaa tgaagcatcc agaaaaaata
     1081 gcgtttatat tgataaagta aacgctctaa aaaaagaaaa taaattatta cttccataca
     1141 aagatcaatt tgatttgaaa ttaaagcagg aaaaagagcc attgcaagca gaagtcaaag
     1201 aattgacttc tgaaaatacc aatttgaaaa atgagaacac aaaattaaaa aaagaaaatt
     1261 tagatttcaa agaatttatt ttgggtgtta aaaattacta tggatctaaa tttcaggaat
     1321 ggtacgagga agtattcaac ccaaaaacaa aaacaaaacc caaattttaa ttgaatattt
     1381 tcatatttgc cgcctatgtg gtgtatgaga caccaccaga agcggcctga cagcgtttag
     1441 ctgtcatagc tgggatttat cccaaaaaaa gtggttttgc acgcagttag ttacctttaa
     1501 atatcagtca cttaaataca aaaaatgcag gttgggaaaa tgtggtcagg gagctggccg
     1561 cattggggtg ggggtacctt cataacgcct tggtgcgttg tgaagagggg gaacggggcg
     1621 gggttccccc tttggtatgt aactacatat aaaacaaaga aatactgtta aactttgaac
     1681 cgtgagaact gcagaaccac ttgtggttct gtggttcgag gggttggcct gcccggcgag
     1741 ggcggttttg cagttgattt tacggtttct tctttcttat tttcttactt cttaagcacc
     1801 ctttaaagcc ttatatatca acactttacc aaaaaaaaaa ttatgaaaat cataattttc
     1861 gattatgaaa atcataatgc aaaattatga aaatcataat cagacttatg aaaatcataa
     1921 ttttgcatac aggtgcattg atatgatttg atgttgaaac gataactttc ttatcgtttt
     1981 gatattgaca ttgcatttag atcggttata aatgcaacat aggttattat ttttattaga
     2041 aaaaggaata taaaatggca aaagcaaaag ttttaataaa cgatacagat gaagcagtaa
     2101 taaaaaacgc attagatgtt gaagatatga acgaagtaaa tattaacgtc acgagaaaaa
     2161 agaaaaccgg atttttaggg acttattcaa tagtatttca tgactctgtg aaaacactat
     2221 ctaaaatgaa cttaaagcca aatgcttata agattgtaat gtacttattc ggcattatag
     2281 agatgggtaa tattttagtt aacttttcac aaaagaagat agctgatgat ttaggcttgc
     2341 agccgtctaa tgtatcaaga gctttcaaag agctttttga taaaggggtt ttaatcaaag
     2401 atagcgaaga cggacacgtt tacttaaatt caaatctatg cactatgggt gttccgaaaa
     2461 attttgatca aaagagaatg aaaagcttac agaaatcaaa tattgaaact gaagatttta
     2521 aaaatcagat taatttattt actccaacaa acaccgcaaa aataaaaact aatgataaaa
     2581 aagttatcaa gaaaaaagaa aaagaaaacg ttttagagtt tccaatgcaa aatgattggg
     2641 ttgatattga atagtaaaac taacactacc gcacttcgtt tgctgaatcc ctaaacctcg
     2701 caaatataaa atttgcaagc tttagggatt tgcttttttg ttttgaagtt gactttaacc
     2761 ccgtagggcc gcatctatgg agcagcatat gaaatatgct agcgtaatag a
//
