LOCUS       NZ_CP072957             3223 bp    DNA     circular CON 14-AUG-2023
DEFINITION  Enterobacter sp. MGH 3 plasmid unnamed4, complete sequence.
ACCESSION   NZ_CP072957
VERSION     NZ_CP072957.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02138543
            Assembly: GCF_018075145.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter sp. MGH 3
  ORGANISM  Enterobacter sp. MGH 3
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Multi-genus dynamics of carbapenem resistance within hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2020) Infectious Disease and Microbiome Program,
            Broad Institute, 415 Main Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.3
            Assembly Name                     :: MGH3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 183x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/14/2023 03:14:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,093
            CDSs (total)                      :: 4,978
            Genes (coding)                    :: 4,853
            CDSs (with protein)               :: 4,853
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 39 of 125
            Pseudo Genes (incomplete)         :: 89 of 125
            Pseudo Genes (internal stop)      :: 22 of 125
            Pseudo Genes (multiple problems)  :: 22 of 125
            Pseudo Genes (short protein)      :: 1 of 125
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072957.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Enterobacter sp. MGH 3"
                     /mol_type="genomic DNA"
                     /strain="MGH 3"
                     /isolation_source="respiratory"
                     /db_xref="taxon:1329812"
                     /plasmid="unnamed4"
     gene            289..774
                     /locus_tag="IBG19_RS25290"
     CDS             289..774
                     /locus_tag="IBG19_RS25290"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            778..993
                     /locus_tag="IBG19_RS25295"
     CDS             778..993
                     /locus_tag="IBG19_RS25295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1161..1493
                     /locus_tag="IBG19_RS25300"
     CDS             1161..1493
                     /locus_tag="IBG19_RS25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            complement(1702..1872)
                     /locus_tag="IBG19_RS25305"
     CDS             complement(1702..1872)
                     /locus_tag="IBG19_RS25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004193911.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNWVHGAEIV
                     ENAYRVLEVKA"
                     /name="hypothetical protein"
     gene            2511..2834
                     /locus_tag="IBG19_RS25310"
     CDS             2511..2834
                     /locus_tag="IBG19_RS25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          2224..2415
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.795"
                     /coverage="89.0"
                     /evalue="1.57e-69"
     Typing          complement(1761..2241)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.867"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          4..1091
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.137"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2825..3222
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.992"
                     /coverage="99.0"
                     /evalue="1.67e-78"
ORIGIN
        1 cctggcagac catagtgaac ggcaggctgg ggctgcatga cccgaacgcg ccggagaacc
       61 ggcgggtgct ggtctcgcct tccgcgctgc cggaagcgaa gcaggaagcc gcccaggcga
      121 ttacgagcgg cctgcttgcc cttgcctcat ccggggagct gaaaacgcgt caggacgtca
      181 ctgaggcgct ggaaagcgca ggttttgagg tggtgcgcac cacaaaaagc agcatcagca
      241 ttgccgaccc ggacgggggg cgaaacatcc gactgaaggg agccatctat gaacagtctt
      301 ttaacgctgg cgaaggactt agagcagaaa tcgaaagcgc agcagcagac taccggcgag
      361 atgctgaaag ccgcattcag cgagcacgag aagtctgtca gagcggaact gagcgaaagc
      421 gagaagagaa tcagcgccgc catcctcgac cacgaccgga agctgtcctc agccatgagc
      481 cagcgcacga aagggatgct gcgcatggtc agccagacgt ggctgaccat cgttctggtc
      541 tccgcgctgc tgatagcgtc gagcgcgggc attctgtggt ggcaggggca gcagatactc
      601 gagaactata cgaccatccg ggagcagaag agcacgcagg ccatgctgtc agagaggaac
      661 agcggcgtac agctctcgac ctgcggcgag cagagacgcc gctgcgtgag ggtgaacccg
      721 gaagcgggac agttcggaga ggactcgagc tggatgatac tggcggggaa atagcacatg
      781 acggagctgg aaaaacagtt gctgagcgca ttagagcagc tacagcagga ctactcgaaa
      841 aggctggacg agtgggagaa cgccttcgcg gaatggcgga cgatgtctgg tcttattcaa
      901 cgggagaacg cggcgctgaa cgagcgcgtc acggtcttga gcaggcaggt gcagagtttg
      961 agcgagcagc tgcgccggtt gtcgaaaggc tgaacgcgat agaaactcac cggcagcagg
     1021 agcgggcggt gcagcaccag aaagcgctgg agctggaacg atcgcagtgg cagcatcatg
     1081 gtccttcgct atgataagcc gggttctatt aattagttgt tatgtaaaga tatttaaatt
     1141 tttcaacatg aggtattttt atgaatacat atattttcct gggaggggct attgtggctg
     1201 aagtcatagg caccaccttt atgaaatatt cagaaggatt tacacgcctg tggccgtctg
     1261 taggaactgc aataagttat tgcgctgcct tttatctgct ttcccaaaca ttggaaaata
     1321 tacctaccgg tattgcttac gcaatttggt ctggtgcggg aattgtacta ataagcctag
     1381 ctggttggtt ggttagcggt caaaaacttg attggcccgc aattgcagga atgttcctta
     1441 tttgcgttgg agttttggtt attaatattt tttcaaaatc ttctgcgcat taacttttta
     1501 ccgaagcata ttggccaaag caaaaggatt tccttgagat cctttctttc tgcgcgtaat
     1561 ctttttccct gtaaacaaaa aaaccaccgc taccaacggt ggttcgtttg ccggaattca
     1621 gagctaccaa ctctttgttc cgatggtaac tggctttcac ggagcgcaga tacctaaact
     1681 gtcctttcag tgtagccgca ctcaggcttt cacttcaaga actctgtagg cattctctac
     1741 aatctctgct ccgtgaaccc agttaccaat ggctaccgcc actggcgatt tgtcgtgtct
     1801 ttccgggttg gactcaagtt gatagttacc ggaacgggcg tggtagtcgg actgaacggg
     1861 gggttcgtgc atacagtcca tcctggagcg aactgcctac ccggaaccga gtttcaggcg
     1921 tggaatgaga aaccgcggcc ataacagcgg aatgacaccg gtaaaccgaa aggcaggaat
     1981 gcgggggagc acgagggagc caccaggggg aaacgcctgg tatctttaag ccgcatcggg
     2041 tttcgccacc gctgatttga gcgtcatttt cgtgatactc gtcagggggg cggagcctat
     2101 ggaaaaacgg caactccgca tccttttctt ggcctttcct gcttcgagcg gcatttgctt
     2161 atcccgtaat ctgtgagctg gtaacgcccg ccgcagtctc acggcaggag cgtagcgacc
     2221 gagtgagcga ggaggcgtca tttcatcacc acctgcattt tgcacttacg cccggcagcc
     2281 gcatttttcc cctgtcacat gaagcacttt cataaattgc cactccgtca taacatgttt
     2341 agccagtata cactccgcta gcgctacgtg actggttcag gactgcgccc cgaaacccgc
     2401 taaaaccact gccgcgcctg cggcttgtcc aacaccgtgc cgaccgggaa aagatttccc
     2461 gtccgtcccg gcgttatcag gaggccggat tggcagacaa acgcagcaaa atgctcacca
     2521 tgtgggtgac tgaggacgag caccggcgtc tgctggagcg ctgcgagggt aagcaactcg
     2581 cggcctggat gcggcagacg tgcctggacg agaagcccgc acgcgccggc aaacttccgt
     2641 cgatctcgcc ggcgctgctt cgtcagcttg ccggcatggg gaacaacctt aaccagattg
     2701 cccgccaggt taacgccggt ggtggaagcg gacacgaccg cgtgcagatt gtcgccgcgc
     2761 tgatggccat cgatgccgga ctcgagcggt tgcggcatgc cgtactggaa aagggtgctg
     2821 atgatgatcg ttaagtttca tcctcggggg cgcggcggcg gcggcgggcc ggtcgattat
     2881 ctgctgggta aagatcgcca gcgcgacggc gccagcgttc tgcaggggaa gccggatgaa
     2941 gtccgggagc ttatcgacgc ctcgccctac gccaaaaagt acacttccgg cgttctgtct
     3001 tttgccgaac aggatttacc gcccggccag cgcgaaaagc tgatggcgag tttcgagcgg
     3061 gttctgatgc ccggactcga taaagaccag tacagcgtgc tgtgggttga gcaccgggac
     3121 aaggggcggc tggagctgaa cttcctgatc ccgaacacgg agcttctgac cggcaagcgg
     3181 ctacagccgt attacgaccg cgccgaccgt ccgcgcatcg atg
//
