LOCUS       NZ_CP072956             4209 bp    DNA     circular CON 14-AUG-2023
DEFINITION  Enterobacter sp. MGH 3 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP072956
VERSION     NZ_CP072956.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02138543
            Assembly: GCF_018075145.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter sp. MGH 3
  ORGANISM  Enterobacter sp. MGH 3
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Multi-genus dynamics of carbapenem resistance within hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2020) Infectious Disease and Microbiome Program,
            Broad Institute, 415 Main Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.3
            Assembly Name                     :: MGH3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 183x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/14/2023 03:14:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,093
            CDSs (total)                      :: 4,978
            Genes (coding)                    :: 4,853
            CDSs (with protein)               :: 4,853
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 39 of 125
            Pseudo Genes (incomplete)         :: 89 of 125
            Pseudo Genes (internal stop)      :: 22 of 125
            Pseudo Genes (multiple problems)  :: 22 of 125
            Pseudo Genes (short protein)      :: 1 of 125
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072956.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Enterobacter sp. MGH 3"
                     /mol_type="genomic DNA"
                     /strain="MGH 3"
                     /isolation_source="respiratory"
                     /db_xref="taxon:1329812"
                     /plasmid="unnamed3"
     gene            complement(join(4169..4209,1..1384))
                     /locus_tag="IBG19_RS25270"
     CDS             complement(join(4169..4209,1..1384))
                     /locus_tag="IBG19_RS25270"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_210954466.1"
                     /translation="MIHKHIGGKKNQRSVKNSLDYLLRTKKPEEQKFVEVLSQTNRSDI
                     ENFNEYIKEKNFKNPFITGVLSFEEKDIDPELKTKIMNEFEEMIFAGIEPENRPPVIWI
                     QHKDKNRLELNYTTFNSLMNKKHFQCYYHLSDKKLFNSFCEKINYENNISSVLDVKEHK
                     ERNTLVNTLNTKIPSDKKAIVEKLQEDILSKIITEEINNREELISFIQSKNIIINRVRE
                     NAISIKFSKEDKPISLKGDIYEQGRDYKTYRTESTIDHRADQQYVENQLRHHRENFDTE
                     LKKRHSRNRTRFKPTQSKDQKNVEYRVKKANDSKNVRSEIPDNNNVPVRNEPDIDFFHN
                     NKTIEDVSNEQNFTNDKTKPREEEPAKFDLSREIETANTQQQFIRSRVEEVRRNQQQFR
                     KSLDRTQERSGVCRRSLHSIIHNIRRYVRTFSIHTRQNERRQKIEHRQKIFLEKLKTQQ
                     EKNNQRSVVRSRFKPY"
                     /name="hypothetical protein"
     gene            complement(1374..1724)
                     /gene="mobC"
                     /locus_tag="IBG19_RS25275"
     CDS             complement(1374..1724)
                     /gene="mobC"
                     /locus_tag="IBG19_RS25275"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_032645810.1"
                     /translation="MPDYKERYQRKNFISLCLSDEELSEVENIADRLNMKRAAAAREIL
                     VTNSKRLKSQIKKNDDAEILFLYSKISNNINQIAKKMNTNLDKFLSGNGEEFSLLIEEI
                     FEDLERLKNNDT"
                     /name="mobC"
     gene            complement(1724..2029)
                     /locus_tag="IBG19_RS25280"
     CDS             complement(1724..2029)
                     /locus_tag="IBG19_RS25280"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032645811.1"
                     /translation="MSHEFLIQRNDNSNEQGYFQGYAEGIFYSAFNCQRFNRGVSGSNE
                     QETFTTAETKSALEKVLNSSEIANYPDPDRISSIKDFYFNIVLNAKKKDIFTVRFF"
                     /name="hypothetical protein"
     gene            complement(2301..3713)
                     /locus_tag="IBG19_RS25285"
     CDS             complement(2301..3713)
                     /locus_tag="IBG19_RS25285"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032645812.1"
                     /translation="MNEDQDEINNLLAEREWEEFKKSSFGKFFTAKQMKEQELTYLNNK
                     ALDIVNEEDLFEIMSLIPQPFITNENIKKADILSVIYNNFIEETEIPLTLPIFSFFSFL
                     SAFCVKNNITYSMPLSEKKSPVNTWVTVLAPSGSAKTYSLGKIEDLIPIKPDGEKVVEQ
                     NFVKPSGPAKFIEEFSNLPDSRDGKSQYGFWIEDEVAQMFKQVEREGTPLSEIKEYFLK
                     AYDNKKLTRKSKKETIETKNLILVLFFINTFDSYVNNISSESMTDGLMRRFNLVYTEKD
                     KSRDFTDFPIYNEKKITDEILSDRFEDLFFNINPDVNYTFSENAFELYRKYFKIFWHRK
                     YKNILTDKENFYRTYMMQSWKFAVFYHLILKKEGTVIDGECLIYGMKISLLFANSMKKF
                     FDFKNKDISDSKREKQKDTLLKYADFLKTKPDATVRDFCRKFSVKKADAVSILKSLECS
                     GIEHSIFEVVSK"
                     /name="hypothetical protein"
ORIGIN
        1 tgtttttttc ttgctgggtt ttgagctttt caaggaagat tttttgtctg tgttcgattt
       61 tttgtcttct ttcattctgt cttgtatgga ttgaaaacgt tctgacgtat cttctaatat
      121 tgtgaataat tgaatgtaga gatcttctac atactccaga tcgttcctga gttctatcaa
      181 gcgactttct gaattgctgt tgatttcttc taacctcttc aactcttgaa cgaatgaatt
      241 gttgttgtgt attagctgtt tcaatttctc ttgatagatc aaattttgct ggttcttctt
      301 ctcttggttt tgttttatca tttgtgaaat tttgctcatt gcttacatcc tcgatagttt
      361 tattattatg aaaaaaatca atatcaggtt cattcctaac aggaacatta ttattgtctg
      421 gtatttcaga tctaacattt ttactgtcat ttgctttttt aactctgtat tcaacatttt
      481 tctggtcttt gctctgtgtt ggcttaaaac gagttctatt tctgctgtgt ctttttttaa
      541 gctctgtgtc aaaattttct ctatggtgtc tgagttggtt ttcgacatac tgctgatctg
      601 ctctgtgatc gattgttgat tctgttctat aagttttgta atctctgccc tgttcatata
      661 tatctccttt cagtgatatt ggtttatctt cttttgaaaa ctttattgat atagcatttt
      721 ctctaactct atttattatt atgtttttag attgaatgaa tgatattaat tcttctctgt
      781 tgtttatttc ttctgtgatt attttcgata aaatatcttc ctgtaatttt tcaacaatgg
      841 cttttttatc tgatggtatt ttagtattta aagtatttac aagagtgttt ctttctttgt
      901 gttctttaac atctaaaaca gaactaatat tattttcata atttattttt tcacaaaatg
      961 agttgaataa ttttttatca gataaatgat aataacattg aaaatgtttt ttattcatta
     1021 aagaattaaa agtcgtatag tttaattcaa gcctgttttt atctttgtgt tgtatccaga
     1081 tcactggtgg gcggttttca ggctctattc ctgcaaaaat catttcttcg aattcattca
     1141 ttatttttgt ttttagttct ggatcgatgt ctttttcttc aaaagataaa acaccagtta
     1201 tgaagggatt tttgaagttt ttttctttta tgtattcgtt gaaattttca atatcagatc
     1261 tgtttgtttg agataaaacc tcaacaaatt tctgttcttc tggtttttta gttcttaata
     1321 aataatcaag tgaattttta acacttcgct gatttttctt tcctccgata tgtttatgta
     1381 tcattatttt ttaacctctc taaatcttcg aatatttctt caatgagtag tgaaaattct
     1441 tctccgtttc ctgataaaaa tttatccaaa ttagtattca tcttttttgc gatctgattt
     1501 atattgtttg agattttaga atataaaaac aatatttcag catcatcatt tttctttatt
     1561 tgtgatttta gtctctttga gtttgtgact aatatttcac gagctgcggc ggctcgtttc
     1621 atatttaacc tatcagcgat attttcaacc tccgataatt cttcatcact caaacaaaga
     1681 gagataaaat tttttctctg atacctctct ttgtaatctg gcattagaaa aatctcactg
     1741 taaaaatatc tttcttttta gcatttaata caatattgaa ataaaaatct ttaatactac
     1801 ttatacgatc aggatcggga tagtttgcta tctcgctaga atttaagact ttttctaatg
     1861 ctgattttgt ttctgctgtg gtgaaagttt cttgttcgtt agaaccagaa acgcctctat
     1921 tgaacctttg gcaattaaag gctgaataga atatgccttc ggcatatcct tggaaatagc
     1981 cttgttcatt gctgttgtca tttctctgga ttaaaaactc gtgactcata ttttgctcct
     2041 tggtttaggt tttgattttg gtttttaggg gattgaaggg ggaacccctc acaagtaaag
     2101 gaatacgaag tattactcta acttgctatc tattacatag ataacaaatt aaaaccttaa
     2161 gtcaagggca aaactgaata taatttgacc ttttataaaa ccgtgagaaa ataaaaaagc
     2221 ccgtaagggc tttattattt ttgaggggtt gaacttgccg aaagaaaatt aaatataaat
     2281 acaaaattta aatcggcaag ttatttgctc acaacttcaa atattgaatg ttctattcct
     2341 gagcattcaa gacttttcaa aattgaaaca gcatctgcct ttttaacact aaattttctg
     2401 caaaaatccc taactgtcgc atctggtttt gttttgagaa aatcagcata tttcaataaa
     2461 gtatctttct gtttttctct ttttgagtct gatatatctt tatttttaaa atcaaaaaac
     2521 tttttcatac tgttggcaaa taacaaagaa attttcattc catatattaa gcattcacca
     2581 tcgatcactg ttccttcttt tttcaaaatc aagtgataga aaactgcaaa tttccagctt
     2641 tgcatcatat atgttctgta aaaattttct ttatcggtaa gaatattttt atattttcta
     2701 tgccaaaaaa tcttgaaata ttttctgtaa agttcaaaag cgttttctga aaaggtataa
     2761 ttaacatctg gattgatatt gaaaaataaa tcttcaaacc tatcagataa aatttcgtct
     2821 gtgatttttt tctcgttgta tataggaaaa tcagtaaaat ctcggctctt atctttttct
     2881 gtataaacaa gattaaatct tctcattaaa ccatctgtca tagattcgga agatatatta
     2941 tttacataac tatcaaaagt gtttatgaaa aataaaacca atataagatt tttagtttct
     3001 attgtctctt ttttgctttt tcttgttaat tttttattgt cataggcttt caagaaatat
     3061 tcttttattt ctgaaagcgg ggttccttcc ctttcaacct gtttgaacat ttgagctact
     3121 tcgtcctcaa tccaaaagcc atattgagat tttccgtctc tggaatctgg aagatttgaa
     3181 aactcttcaa taaattttgc tgggcctgac ggcttaacaa aattttgctc aacaactttt
     3241 tcgccatcag gttttattgg tattaagtcc tctatttttc ccaatgagta agtttttgcc
     3301 gatcctgacg gtgctaaaac cgttacccat gtgttaactg gtgatttttt ttcagataaa
     3361 ggcattgaat atgtaatatt attttttaca caaaaagcac ttaaaaaact aaaaaacgaa
     3421 aatatcggaa gtgttaaagg tatttctgtt tcttcaatga aattattgta tataacactc
     3481 aaaatatcag ccttttttat attttcattg gtaataaaag gctgtggtat taatgacatt
     3541 atttcgaata aatcttcttc gtttacaata tccaatgctt tattgtttaa ataagttaat
     3601 tcctgttctt tcatttgttt tgccgtaaag aatttaccaa atgaagattt tttaaattcc
     3661 tcccattctc tttcagctaa caagttgttt atttcgtctt gatcttcgtt catattaaaa
     3721 ccctctaaat ttattttata taatatatat acaatataaa tttaacttgt caaggttttt
     3781 aaagaaaaac tccgacacgt ttttaacttt ttttctgaca gtccgacatt ctgacaaaat
     3841 gcctttaact attgatttat aagggttttt cgtaaaaaaa cggttctgac aaagttctga
     3901 caaagttctg acaaaatccc tgacaggttt ctgacgagca aaatatgatt attttttaaa
     3961 cttgcacgaa gtgggggagg ggaaaaaata gtcagttttc ttcttttggg tttagtgcag
     4021 ttaacaagcg agttttacac cagtgatcta tgtatgttgg ttttcgcttg ttcaacccct
     4081 cacaagcaaa aaccagcctc tggctggttc tcacttgttc acggttaagg ttttgtcgta
     4141 gtgggttaga agagtattca gaacggttct aatacggttt aaatcgactt ctgacgacac
     4201 ttctttgat
//
