LOCUS       NZ_CP072955             4866 bp    DNA     circular CON 14-AUG-2023
DEFINITION  Enterobacter sp. MGH 3 plasmid unnamed2, complete sequence.
ACCESSION   NZ_CP072955
VERSION     NZ_CP072955.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02138543
            Assembly: GCF_018075145.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter sp. MGH 3
  ORGANISM  Enterobacter sp. MGH 3
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Multi-genus dynamics of carbapenem resistance within hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2020) Infectious Disease and Microbiome Program,
            Broad Institute, 415 Main Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.3
            Assembly Name                     :: MGH3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 183x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/14/2023 03:14:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,093
            CDSs (total)                      :: 4,978
            Genes (coding)                    :: 4,853
            CDSs (with protein)               :: 4,853
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 39 of 125
            Pseudo Genes (incomplete)         :: 89 of 125
            Pseudo Genes (internal stop)      :: 22 of 125
            Pseudo Genes (multiple problems)  :: 22 of 125
            Pseudo Genes (short protein)      :: 1 of 125
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072955.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Enterobacter sp. MGH 3"
                     /mol_type="genomic DNA"
                     /strain="MGH 3"
                     /isolation_source="respiratory"
                     /db_xref="taxon:1329812"
                     /plasmid="unnamed2"
     gene            complement(join(4233..4866,1..218))
                     /locus_tag="IBG19_RS25230"
     CDS             complement(join(4233..4866,1..218))
                     /locus_tag="IBG19_RS25230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_032645796.1"
                     /translation="MFKIENNKDILKSQIPLKPYATNDLDFGLTIQNKNKALDMLYLQV
                     NHPLYLHTMVFDLDKENCFYEFENAHLPIPSFITKSPDSGRCHYGYMLNAPITSTEKSR
                     QKPVKFARALYYNMATRLGADLGYAGLITKNPYSPHWSPFWSGADLYELNDLADCFDDL
                     EEPKKRENTGFAFGRNVEMFDTIRQWAYKNVLKYQSESSFNDFHNELLLKCQMHNAYIN
                     TDDLLPYNEIKATAKSIAKFCWKEFSEEKLNKIQSKKQSYRGKKNKGIVKSKTRKFLEA
                     IK"
                     /name="replication initiation protein"
     gene            509..733
                     /locus_tag="IBG19_RS25235"
     CDS             509..733
                     /locus_tag="IBG19_RS25235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032645795.1"
                     /translation="MLEQKDKKRRLGFMIGKSNATLPADEQTEAELDQEVLKLFYPNQY
                     TKDTIERSEAGTDVFTASDSDDLFKKLKL"
                     /name="hypothetical protein"
     gene            1347..1613
                     /locus_tag="IBG19_RS25240"
     CDS             1347..1613
                     /locus_tag="IBG19_RS25240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032645794.1"
                     /translation="MNIVLSVSKCLYINDLDRLLPCDRFFVHLDYRDISKKESFEMNRH
                     DKETYKLIFDKLVGNDVMLAIDEDIYSEVVENLLYYSNKTYGD"
                     /name="hypothetical protein"
     gene            1620..1886
                     /locus_tag="IBG19_RS25245"
     CDS             1620..1886
                     /locus_tag="IBG19_RS25245"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_155858589.1"
                     /translation="MIDEMTQYRIRRFTQSQKSLLLKMIKERQLRDLEDNIKDILKDID
                     VSYSLGITKKASRKEEMIACREVHHIFSILTKRDINNDYRNRI"
                     /name="hypothetical protein"
     gene            1888..2142
                     /locus_tag="IBG19_RS25250"
     CDS             1888..2142
                     /locus_tag="IBG19_RS25250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_247687638.1"
                     /translation="MKLNVLARFFYYIEQDKDIPFSEINIDERDLCYFVANRYITENKA
                     DELIEALIIENDDDYIRATEDYIIMRNKKCQQQTEDEDV"
                     /name="hypothetical protein"
     gene            2109..2486
                     /gene="mobC"
                     /locus_tag="IBG19_RS25255"
     CDS             2109..2486
                     /gene="mobC"
                     /locus_tag="IBG19_RS25255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_032645791.1"
                     /translation="MPTTDRRRGRINLNADERRDISIQFRVNKAEFEYLKSLVEASGLE
                     RGHYIRKEILNSEPVIKRKMPEVNQKTYMQLSAIGNNINQAVKKLNGNEYFDKNEVDKL
                     NFNIKQVSRRIIEINKDLGYI"
                     /name="mobC"
     gene            2483..3958
                     /locus_tag="IBG19_RS25260"
     CDS             2483..3958
                     /locus_tag="IBG19_RS25260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_210954465.1"
                     /translation="MKGNVAKTGRSFKNRVDYILKDDHSFICSNMSADKNNVSDLTDEF
                     KTVSSFRQDIKKPVFHAYLSLPKNEHLTDEKWQEIAKDYLKEMNIDIEKHQYICVRHND
                     TDKEHIHIVANRVGLDGSVWLGQHSAFNTIAACERLEVKHSLTITQSLKGQKSDVSAPT
                     KNEIEQALRKGEKPARLVLQNALQAALVGKPDLSTFVERLQAVGIEPRFNVASTGNVAG
                     CSFSVGDVAFKGSQLGKKFSWNTIKGKVKYDKNRDDELVRSFSARKDDEPNSIGRSLVE
                     SNINDSRAVDGIDDDIIRISQHSIKSDTTDTNKSEQLIRSNNNSFKLSEKYNRPTNKKI
                     GRATHKAAQILNRVKKQYNKEPIKTYNKNGLNSWRDKSDSGSISRILDHAETATTATNN
                     FRLKGRRNINSRATKIEEIRRRLGIEKPVIQPKKIVKEEIKKKPLGFSMDENRFKSKLK
                     EAIKKEKPKNETTPTQLQQEDDQHLKPRGFKPR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3967..4236)
                     /locus_tag="IBG19_RS25265"
     CDS             complement(3967..4236)
                     /locus_tag="IBG19_RS25265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032645797.1"
                     /translation="MKVKNRKRELTAKEYAEQYEISVRTVKRYFSQDREDYEKEAKQRR
                     LKAFVLRESGMKWSDVGIALGTTKHGAIALYKRYQQIDAPIKEA"
                     /name="hypothetical protein"
ORIGIN
        1 ggcaaatggg cattttcaaa ttcataaaag cagttttctt tgtctaaatc aaataccatt
       61 gtatgaagat aaagcgggtg atttacttgt aaataaagca tatctaaagc tttattctta
      121 ttttgaatag ttaatccaaa atcgagatca tttgtagcat atggctttaa aggtatttga
      181 ctttttaata tatctttgtt attttctatc ttgaacatat agcagtatcc tttctgtaat
      241 gccccgttct ctcactggtc gccaaactaa tcagagaacg cgggcttttc ttttatttat
      301 aatcttttac agttttacta taccaaaaaa acttgtcaat agatccgtca aaaaaagttg
      361 ttatgtgtgc cattaaccaa tatcaggtat tagacggagt ttttggaaaa ctacaccaca
      421 gcgaagcaca ggccgaaggc taccgcagag taaagtaacg tatactaaac ttcatatgca
      481 ggatctggct gtgctgggga ggtaaatcat gctcgaacag aaggacaaga aacggcggtt
      541 aggttttatg attggtaaga gcaatgcaac attgccagca gacgaacaaa cagaagcaga
      601 gcttgatcag gaagtcctga agctgtttta tcctaaccaa tacaccaaag acaccataga
      661 acgctcagaa gccggtacag acgttttcac ggcctccgat tctgatgacc tgttcaaaaa
      721 attgaagctg tagagggtag gggagagctg gccatcttca ccagttgaga acaaaccgac
      781 ccggaacggt gagagaaggc caaccctcga tgcagtatat actgatttat actaatctat
      841 agtgttgatt ttatagccat ttcaaaacca tagacgccag caacagcaga aaagcaagtt
      901 ttctactgtt ctgtcgccgc cgatcaaacc ccatttctta aaatctgcct accaagtggt
      961 cacgaacttt tccagatttc acgtagcagc ggcatttttt acgcaaagct acttattacg
     1021 cacagctacc catcaagaca atatattatt taccctattt ttatttgcat tttatttgtc
     1081 gtgcagtgta tttatttatg tagccgctat attgacaaac tttttagatc ggttatttat
     1141 ttaaatatag gttgagttca gctttatttt tagcttttcg gaaactgagg caaggtgtaa
     1201 tcccgtagcc ggtattatac ccaacctttc aggttgcccc ctctcacttc gttcggaggg
     1261 ttgttaaaag atctaccctt ccgcctattc cccttgtcaa aggggaggcg gtaaatagtt
     1321 ttacatcaaa acaaaaagga ataaatatga atatagtttt atcagtatca aaatgtttat
     1381 acataaacga tttagatcgc ttacttccgt gtgatcgttt ttttgttcat ttagattata
     1441 gagatattag caaaaaagag tcatttgaga tgaatagaca cgacaaagaa acatacaaac
     1501 ttatctttga taaacttgta ggtaatgacg taatgcttgc tattgatgaa gacatctaca
     1561 gcgaagttgt cgaaaactta ctttattatt caaataagac ttatggagat taatataaga
     1621 tgatagatga aatgacacaa tacagaataa gacgctttac acaaagtcaa aaaagcttgc
     1681 tactaaagat gataaaagaa aggcagctaa gagatttaga agacaacata aaagatattt
     1741 taaaagatat tgatgttagt tattcattag gcataacaaa gaaagcatca agaaaagaag
     1801 aaatgatcgc ttgtcgtgaa gttcatcaca tctttagtat attaacaaaa agagacatca
     1861 ataatgatta tagaaataga atatgaaatg aaacttaatg tattagcacg ttttttttac
     1921 tacatagaac aagataaaga tataccattt agtgaaataa atatcgatga gcgggattta
     1981 tgttattttg tagcaaatag atacattaca gaaaataaag ctgatgaact tattgaagca
     2041 ttgattattg aaaatgatga cgactacata agagcaacag aggattacat tattatgagg
     2101 aataaaaaat gccaacaaca gacagaagac gaggacgtat aaatttaaat gctgacgaac
     2161 gcagagatat atctatacag tttagagtaa acaaagcaga gtttgaatat ttaaaatcat
     2221 tagtggaagc ctccggcctt gagagaggtc attacatccg aaaagagatt ttaaactctg
     2281 agccagttat taaaaggaaa atgccggaag taaatcaaaa aacatatatg cagctttcag
     2341 ctatagggaa caatataaat caggctgtta aaaagttaaa tggcaatgag tattttgata
     2401 aaaatgaagt tgataaactc aactttaata taaagcaagt aagcagaagg ataatagaaa
     2461 taaataaaga cttggggtat atatgaaagg gaatgtagcg aaaacgggaa gatcatttaa
     2521 aaatagagtt gattacatat taaaagatga tcacagtttt atttgtagca atatgtcagc
     2581 agataaaaat aatgttagtg atttaactga tgagttcaag actgtaagca gctttagaca
     2641 agatataaaa aaaccagtgt ttcatgcata tttatctctt ccaaaaaatg aacatttaac
     2701 agatgagaaa tggcaagaga tagcaaaaga ctatttgaaa gaaatgaata ttgatataga
     2761 aaaacatcaa tatatttgtg tgcgtcataa cgatacagat aaagaacata tccatatagt
     2821 agcaaataga gtcggtttag atggttcggt atggcttggg cagcactcag catttaatac
     2881 aatcgcagct tgtgaacgtt tagaagtaaa gcacagctta accattacac aaagtctcaa
     2941 aggccaaaaa tcagacgtat cagcaccaac caagaacgag atcgagcaag ccctacgcaa
     3001 aggtgagaaa ccagctcgtt tagtgctaca gaacgcacta caagcggctt tagtcggtaa
     3061 gcctgatttg tccacgttcg tagaacgtct gcaggccgta ggcattgaac ctcggttcaa
     3121 tgttgccagc actggcaacg tggcgggttg ttctttcagt gtgggagatg tggcattcaa
     3181 gggatctcag ttaggtaaaa aatttagttg gaacactatt aaaggcaagg tgaaatatga
     3241 caaaaataga gatgatgaac tcgttaggag cttctcagca agaaaagatg atgaaccaaa
     3301 tagtattgga cgatcacttg ttgaatcaaa tatcaatgac agtagagccg ttgacggaat
     3361 cgatgatgat attatccgaa tcagtcagca cagcattaaa tcagacacaa cagacactaa
     3421 caagagtgaa caactcatta gaagcaacaa caacagcttt aaactcagcg aaaaatataa
     3481 tagaccaaca aacaaaaaga ttggaagagc aacacataaa gctgcacaaa tactcaacag
     3541 agttaaaaaa caatacaata aagaaccaat caaaacttat aataaaaatg gcctcaatag
     3601 ttggcgtgat aaatctgata gcggttctat cagtaggata cttgatcatg cagaaacagc
     3661 cacaacagcc acgaataact ttagactcaa aggccgtcgc aacatcaata gtagagcaac
     3721 taagatcgaa gaaataaggc gtaggcttgg aatagaaaaa cctgttattc agccaaagaa
     3781 aatagttaaa gaagaaataa agaaaaagcc tttaggtttt tctatggatg aaaatagatt
     3841 taaatcgaag ttaaaagaag ctattaaaaa agaaaaacca aaaaatgaaa caacaccaac
     3901 acaactacag caggaagacg atcagcattt aaaaccaaga ggttttaaac cgcgataaag
     3961 gaggctttaa gcctctttta ttggtgcgtc tatttgttga taacgtttat aaagagctat
     4021 ggcaccgtgt ttagtagttc caagagcaat accaacatca gaccatttca taccgctttc
     4081 acgtaataca aaagctttta agcgtctttg ttttgcttct ttctcatagt cttcacgatc
     4141 ttgagaaaaa tatcgtttaa ctgtcctaac gctaatctca tactgttcag catactcttt
     4201 tgctgtaagt tctctttttc tatttttaac tttcatttta tcgcctccaa aaacttacga
     4261 gttttacttt ttactattcc tttgtttttc tttccacggt aagactgttt tttactctgt
     4321 attttgttga gtttttcttc gctaaactct ttccagcaaa actttgctat actttttgct
     4381 gtggctttta tctcattata tggcagtaaa tcatcagtat ttatatatgc attatgcatt
     4441 tgacacttta gcaatagttc attgtgaaaa tcattaaatg agctttcact ctgatatttt
     4501 agcacatttt tataagccca ttgccttatg gtgtcgaaca tttcaacatt acgcccaaaa
     4561 gcaaaccccg tattttctcg tttttttggt tcttctaaat catcaaagca atcagctaaa
     4621 tcatttaact catataaatc agcacctgac cagaacggcg accaatgcgg gctgtatgga
     4681 tttttagtaa tcagtccagc ataccccaga tcagcaccta aacgagtagc catattgtaa
     4741 tataatgctc tggcaaactt tacgggcttc tgtctgcttt tctctgttga tgttatagga
     4801 gcattaagca tataaccata atggcaacgt ccactatcag ggcttttagt tataaaagat
     4861 ggtata
//
